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5. Monkeypox virus induces the synthesis of less dsRNA than vaccinia virus, and is more resistant to the anti-poxvirus drug, IBT, than vaccinia virus

Author

Arndt, William D., primary, White, Stacy D., additional, Johnson, Brian P., additional, Huynh, Trung, additional, Liao, Jeffrey, additional, Harrington, Heather, additional, Cotsmire, Samantha, additional, Kibler, Karen V., additional, Langland, Jeffrey, additional, and Jacobs, Bertram L., additional

Published
2016
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7. Detecting Necroptosis in Virus-Infected Cells.

Author

Cotsmire SM, Szczerba M, and Jacobs BL

Subjects
Acrylamides pharmacology, Animals, Benzothiazoles pharmacology, Cell Line, Cell Survival drug effects, Cross-Linking Reagents chemistry, Fibroblasts drug effects, Fibroblasts metabolism, Fluorescent Dyes chemistry, Gene Expression Regulation, Humans, Imidazoles pharmacology, Indoles pharmacology, Necroptosis drug effects, Oligopeptides pharmacology, Phosphorylation drug effects, Protein Kinases metabolism, Protein Multimerization drug effects, Quinolines pharmacology, Receptor-Interacting Protein Serine-Threonine Kinases antagonists & inhibitors, Receptor-Interacting Protein Serine-Threonine Kinases metabolism, Succinimides chemistry, Sulfonamides pharmacology, Vaccinia virus growth & development, Fibroblasts virology, Immunoblotting methods, Necroptosis genetics, Protein Kinases genetics, Receptor-Interacting Protein Serine-Threonine Kinases genetics
Abstract

Necroptosis has been implicated as a critical cell death pathway in cancers, Alzheimer's and other neurodegenerative diseases, and virus-infected cells. Necroptosis occurs when mixed-lineage kinase domain-like protein (MLKL) punctures the cytoplasmic membrane allowing a rapid influx of water leading to a loss of cellular integrity. As its role in human disease becomes apparent, methods identifying necroptosis will need to be further developed and optimized. Here we describe identification of necroptosis through quantifying cell death with pathway inhibitors and using western blots to identify end points of MLKL activation and protein-protein interactions leading to it.

Published
2021
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