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The reactionsK−p→π∓Σ(1385)± are studied at an incident laboratory momentum of 8.25 GeV/c using data from a high statistics (≃180 events/μb) bubble chamber experiment. In the case of the reactionK−p→π−Σ(1385)+ an amplitude analysis is performed and the complete Σ(1385)+ spin density matrix is extracted as a function oft′. The results are compared with the predictions of the additive quark model. In the case of the reactionK−p→π+Σ(1385)− the cross-sections for forward and backward production are determined.

Very long-chain acyl-CoA dehydrogenase (VLCAD) is one of four flavoproteins which catalyze the initial step of the mitochondrial beta-oxidation spiral. By sequence comparison with other acyl-CoA dehydrogenases, Glu-422 of VLCAD has been presumed to be the catalytic residue that abstracts the alpha-proton in the alphabeta-dehydrogenation reaction. Replacing Glu-422 with glutamine (E422Q) caused a loss of enzyme activity by preventing the formation of a charge transfer complex between VLCAD and palmitoyl-CoA. This result provides further evidence for Glu-422 being part of the active site of VLCAD. F418L is a disease-causing mutation in human VLCAD deficiency. Unlike wild-type VLCAD, F418L and F418V contained no bound FAD when expressed at extremely high levels in the baculovirus expression system. Although F418T and F418Y bound FAD at a level similar to that of wild-type VLCAD, both showed reduced Vmax values toward palmitoyl-CoA, most likely due to a decrease in the rate of enzyme-bound FAD reduction. These data suggest that Phe-418 is involved in the binding and subsequent reduction of FAD. FAD-deficient VLCADs (F418L, F418V, and apo-VLCAD) showed increased sensitivity to trypsinization. Loss of FAD may change the folding of VLCAD subunit.

Fifty-five constant-strain-rate triaxial tests were performed on verticaly oriented multi-year pressure ridge samples from the Beaufort Sea. The tests were performed on a closed-loop electrohydraulic testing machine at two nominal strain rates (10−5 and 10−3 s−1) and two temperatures (−20° and −5°C). In all of the tests the confining pressure was ramped in constant proportion to the applied axial stress (σ1 > σ2 = σ3, σ3/σ1 = constant). Two σ3/σ1 ratios were investigated: 0.25 and 0.50. This paper summarizes the sample preparation and testing techniques used in this investigation and presents data on the confined compressive strength and failure strain of the ice. Uniaxial data are also included for comparison.

Data are presented on the variation of ice structure, salinity, and density in multi-year pressure ridges from the Beaufort Sea. Two continuous multi-year pressure ridge cores are examined as well as ice sample data from numerous other pressure ridges. The results suggest that the large scale properties of multi-year pressure ridges are not isotropic, and that the use of anisotropic ridge models may result in lower design ridge ice loads.

Thirty-six constant strain-rate uniaxial tension tests were performed on vertically oriented multi-year pressure ridge samples from the Beaufort Sea. The tests were performed on a closed-loop electro-hydraulic testing machine at two strain rates (10−5 and 10−3 s−1) and two temperatures (−20° and −5°C). This paper summarizes the sample preparation and testing techniques used in the investigation and presents data on the tensile strength, initial tangent modulus, and failure strain of the ice.

A series of 222 uniaxial constant-strain-rate compression tests was performed on vertical multi-year pressure ridge sea ice samples. A preliminary analysis of the effect of structure on the compressive strength of the ice was performed on 78 of these tests. Test parameters included a temperature of −5°C (23°F) and strain rates of 10−5 and 10−3 s−1. Columnar ice loaded parallel to the elongated crystal axes and perpendicular to the crystal c-axis was consistently the strongest type of ice. The strength of the columnar samples decreased significantly as the orientation of the elongated crystals approached the plane of maximum shear. Samples containing granular ice or a mixture of granular and columnar ice resulted in intermediate and low strength values. No clear relationship could be established between structure and strength for these ice types. However, in general, their strength decreased with an increase in porosity.

Over two hundred unconfined compression tests were performed on vertical ice samples obtained from 10 multi-yr pressure ridges in the Beaufort Sea. The tests were performed on a closed-loop electrohydraulic testing machine at two strain rates (10−5 and 10−3 s−1) and two temperatures (−20° and −5°C). This paper summarizes the sample preparation and testing techniques used in the investigation and presents data on the compressive strength and initial tangent modulus of the ice.

Monoclonal antibodies were generated to a purified preparation of the fascia adherens domains of the intercalated discs of chicken cardiac cell membranes. One of these antibodies, McAb 20, immunofluorescently labeled the Z lines of adult skeletal muscle, the Z lines and intercalated discs of adult cardiac muscle, and the dense bodies and dense plaques of adult gizzard smooth muscle. In addition, McAb 20 was found to label regenerating muscle cells in a cross-striated pattern much like that of Z lines in 24-h muscle cell cultures before the appearance of Z lines was detectable by phase or Nomarski optics and before the concentration of alpha-actinin occurred at the Z lines. Thus, McAb 20 appears to be directed against an antigen involved in early myofibrillar organization. Preliminary biochemical characterization of the antigen recognized by McAb 20 indicates that it is a high molecular weight doublet of over 5 X 10(5) kD that is highly susceptible to proteolysis. By virtue of its presence in Z lines, and its possible role in the end-on attachment of microfilaments to Z lines and membranes, we have named this protein zeugmatin (xi epsilon nu gamma mu alpha identical to yoking).

The production ofK* resonances has been studied in the reaction at 8.25 GeV/c. The data comes from a high statistics bubble chamber experiment (180 events/μb). Masses, widths and production cross-sections have been determined for the first threeK*'s. The contributions from natural and unnatural parity exchange have been obtained for theK*(890) and theK*(1420). A partial wave analysis of theK π system from threshold to 1.9 GeV provides evidence for a 0+ enhancement near 1.4 GeV which could be interpreted as the κ(1350).

A partial wave analysis of theK system produced by 8.25 GeV/cK− mesons in the reaction has been performed, taking into account the information provided by the Λ0 decay. Thef′ region is dominated byD0(−) andD1(+) waves. We see no evidence for the production of new 0++ states in the mass region 1.05 to 1.75 GeV.

1. A method of stabilizing the enzyme by using glycerol is described. 2. A purification procedure is presented giving a higher purification than previously described. 3. Data showing substrate activation and activation by citrate are presented. 4. Kinetic constants for NAD+, NADH and certain bivalent metal ions are given. 5. Pronounced inhibitory buffer effects are described. 6. A brief comparison between the NAD-specific isocitrate dehydrogenase from peas and that from other sources is made.

1. The effect of pH on the co-operative activation of the NAD-specific isocitrate dehydrogenase from pea mitochondria by isocitrate is shown. 2. The interlinked effects of pH on the affinity of the NAD-specific isocitrate dehydrogenase for isocitrate and the dependence of the pH optimum on the substrate concentration are presented. 3. A consideration of the conditions of pH and substrate concentration under which citrate activates the NAD-specific isocitrate dehydrogenase demonstrates similarities between the binding of isocitrate and citrate. 4. A comparison of the effects of citrate and pH on the gross structure of the enzyme is investigated by density-gradient centrifugation. 5. The kinetic interpretations of these results are briefly considered. 6. The metabolic significance of these studies is discussed.

1. The effect of pH on the Vmax. and concentration of NAD+ at half-maximum velocity at a constant isocitrate concentration was examined, and the results were related to the requirements for binding of H+ ions to the enzyme. 2. The effect of varying the NAD+ concentration on the pH optimum with constant isocitrate concentration was studied. 3. A comparison has been made between the effect of isocitrate concentration on the characteristics of binding of NAD+ and the effect of NAD+ concentration on the characteristics of isocitrate binding at three different pH values. 4. The mechanistic and metabolic significance of these studies is considered.