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14 results on '"Crippa, Cecilia"'

2. Occurrence and genomic characterization of antimicrobial-resistant and potential pathogenic Escherichia coli from Italian artisanal food productions of animal origin.

Author

Crippa, Cecilia, De Cesare, Alessandra, Lucchi, Alex, Parisi, Antonio, Manfreda, Gerardo, and Pasquali, Frédérique

Subjects
*FOOD of animal origin, *GOURMET foods, *ESCHERICHIA coli, *ITALIAN cooking, *WHOLE genome sequencing
Abstract

Escherichia coli can harbor a broad repertoire of virulence and antimicrobial resistance (AMR) genes, which can be exchanged across the human gastrointestinal microflora, thus posing a public health risk. In this study, 6 batches of artisanal soft cheese and a 6-month ripened fermented dried sausage were investigated to assess the occurrence, phylogeny, and genomic traits (AMR, virulence, and mobilome) of E. coli. 30 and 3 strains isolated from salami and cheese food chains, respectively, were confirmed as E. coli by whole genome sequencing. The accumulation of single nucleotide polymorphism differences within small clusters of strains encompassing batches or processing stages, combined with high serotype and phylogroup diversity, suggested the occurrence of different contamination phenomena among the facilities. A total of 8 isolates harbored plasmid-mediated resistance genes, including one cheese strain that carried an IncQ1 plasmid carrying AMR determinants to macrolides [mph(B)], sulfonamides (sul1, sul2), trimethoprim (dfrA1), and aminoglycosides [aph(3")-Ib and aph(6)-Id]. A pool of virulence-associated genes in the class of adhesion, colonization, iron uptake, and toxins, putative ColV-positive iron uptake systems sit, iro, or iuc (8 salami and 2 cheese), plasmid-encoded hemolysin operon hlyABCD (one salami), and potential atypical enteropathogenic E. coli (3 salami environment) were reported. Overall, our findings underscore the importance of routine surveillance of E. coli in the artisanal food chain to prevent the dissemination of AMR and virulence. [ABSTRACT FROM AUTHOR]

Published
2024
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5. Artisanal food productions of animal origin: exploring food safety in the age of Whole Genome Sequencing

Author

Manfreda, Gerardo, Crippa, Cecilia <1994>, Manfreda, Gerardo, and Crippa, Cecilia <1994>

Abstract

The artisanal food chain is enriched by a wide diversity of local food productions with delightful organoleptic characteristics and valuable nutritional properties. Despite their increasing worldwide popularity and appeal, several food safety challenges are addressed in artisanal facilities context suffering from less standardized processing conditions. In such scenario, recent advances in molecular typing and genomic surveillance (e.g., Whole Genome Sequencing [WGS]) represent an unprecedent solution capable of inferring sources of contamination as well as contributing to food safety along the artisanal food continuum. The overall objective of this PhD thesis was to explore potential microbial hazards among different artisanal food productions of animal origins (dairy and meat-derived) typical of the food culture and heritage landscape belonging to Mediterranean countries. Three different studies were then carried out, specifically focussing on: 1) compare the seasonal variability of microbiological quality and potential occurrence of microbial hazards in two batches of Italian artisanal fermented dairy and meat productions; 2) Investigate genetic relationships as well as virulome and resistome of foodborne pathogens isolated within dairy and meat-derived productions located in Italy, Spain, Portugal and Morocco; 3) investigate the population structure, virulome, resistome and mobilome of Klebsiella spp. isolates collected from study 1, including an extended range of public sequences.

Published
2023

7. Resistome and virulome diversity of foodborne pathogens isolated from artisanal food production chain of animal origin in the Mediterranean region

Author

Pasquali, Frédérique, primary, Gambi, Lucia, additional, De Cesare, Alessandra, additional, Crippa, Cecilia, additional, Cadavez, Vasco, additional, Gonzales-Barron, Ursula, additional, Valero, Antonio, additional, Achemchem, Fouad, additional, Lucchi, Alex, additional, Parisi, Antonio, additional, and Manfreda, Gerardo, additional

Published
2022
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10. Survey on Carbapenem-Resistant Bacteria in Pigs at Slaughter and Comparison with Human Clinical Isolates in Italy

Author

Bonardi, Silvia, primary, Cabassi, Clotilde Silvia, additional, Manfreda, Gerardo, additional, Parisi, Antonio, additional, Fiaccadori, Enrico, additional, Sabatino, Alice, additional, Cavirani, Sandro, additional, Bacci, Cristina, additional, Rega, Martina, additional, Spadini, Costanza, additional, Iannarelli, Mattia, additional, Crippa, Cecilia, additional, Ruocco, Ferdinando, additional, and Pasquali, Frédérique, additional

Published
2022
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12. Impacts on production performances and costs from the development of antibiotic-free poultry farming, a case study in Italy

Author

Frederique Pasquali, Massimo Canali, Crippa, Cecilia, Gambi, Lucia, MAURIZIO ARAGRANDE, Gerardo Manfreda, Caetano Luiz Beber, and Frédérique Pasquali, Massimo Canali, Cecilia Crippa, Lucia Gambi, Maurizio Aragrande, Gerardo Manfreda, Caetano Luiz Beber

Subjects
Antibiotic-free meat, antibiotic-free poultry, antibiotic-free labels, Antimicrobial resistance in livestock farming, poultry farming, broiler production
Published
2021

13. One Health Perspective on Infections in Nephrology: A Parallel Survey on Carbapenem- and Multidrug-resistant Klebsiella Pneumoniae and Pseudomonas Aeruginosa in High-risk Renal Patients and Farm Animals in Italy

Author

Bonardi, Silvia, primary, Fiaccadori, Enrico, additional, Parisi, Antonio, additional, Manfreda, Gerardo, additional, Cavirani, Sandro, additional, Cabassi, Clotilde Silvia, additional, D’Errico, Michele Luca, additional, Pupillo, Giovanni, additional, Sabatino, Alice, additional, Spadini, Costanza, additional, Crippa, Cecilia, additional, Pizzin, Gisella, additional, Ruocco, Ferdinando, additional, and Pasquali, Frédérique, additional

Published
2021
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14. The resistome of commensal Escherichia coliisolated from broiler carcasses “produced without the use of antibiotics”a

Author

Gambi, Lucia, Crippa, Cecilia, Lucchi, Alex, De Cesare, Alessandra, Parisi, Antonio, Manfreda, Gerardo, and Pasquali, Frederique

Abstract

Several strategies have been in place in food animal production to reduce the unnecessary use of antimicrobial agents. Beyond the monitoring of their use, the evaluation of the effect of these strategies on the occurrence and types of antimicrobial resistance (AMR) associated genes is crucial to untangle the potential emergence and spread of AMR to humans through the food chain. In the present study, the occurrence of these genes was evaluated in commensal Escherichia coliisolated from broiler carcasses “produced without the use of antibiotics” in 3 antibiotic-free (AB-free) farms in Italy in 2019. Sequenced data were analyzed along with publicly available genomes of E. colicollected in Italy from the broiler food chain from previous years (2017–2018). The genetic relationships among all 93 genomes were assessed on de novoassemblies by in silicoMLST and SNP calling. Moreover, the resistomes of all genomes were investigated. According to SNP calling, genomes were gathered in three clades. Clade A encompassed, among others, ST117, ST8070, and ST1011 genomes. ST10 belonged to clade B, whereas Clade C included ST58, ST297, ST1101, and ST23 among others. Regarding the occurrence of AMR genes, a statistically significant lower occurrence of these genes in the genomes of this study in comparison to the public genomes was observed considering the whole group of genes as well as genes specifically conferring resistance to aminoglycosides, β-lactams, phenicols, trimethoprim, and lincosamides. Moreover, significant reductions were observed by comparing the whole group of AMR associated mutations, as well as those specifically for fluoroquinolones and fosfomycin resistance. Although the identification of 3° generation cephalosporin resistance associated genes in AB-free E. coliis a concern, this study provides a first indication of the impact of a more prudent use of antimicrobial agents on the occurrence of AMR genes in Italian broiler production chain. More studies are needed in next years on a higher number of genomes to confirm this preliminary observation.

Published
2022
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