8 results on '"Cristian Pérez-Corrales"'
Search Results
2. Diarrheagenic Escherichia coli in Costa Rican children: a 9-year retrospective study
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Cristian Pérez-Corrales and Kevin Leandro-Sandí
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Diarrheagenic Escherichia coli ,Children ,Gastroenteritis ,Medicine ,Biology (General) ,QH301-705.5 ,Science (General) ,Q1-390 - Abstract
Abstract Objectives This study aimed to estimate diarrheagenic Escherichia coli (DEC) prevalence among pediatric patients with diarrhea at the Costa Rican National Children’s Hospital-Social Security Service (Hospital Nacional de Niños-Caja Costarricense del Seguro Social; HNN-CCSS). DEC variations with respect to rainfall, presence of coinfections, and DEC antimicrobial resistance were also investigated. Results A retrospective observational study from January 2008 to December 2016 was conducted. A total of 12 247 gastroenteritis records were analyzed. Annual DEC prevalence ranged from 2.7% (2008) to 9.0% (2013). The most prevalent pathotypes were enteroaggregative E. coli (EAEC) [n = 189 (31%)], enteropathogenic E. coli (EPEC) [n = 145 (24%)] and enteroinvasive E. coli (EIEC) [n = 91 (15%)]. A reduction in the probability of EAEC gastroenteritis was detected as rainfall rose above 200 mm/mo. [(Generalized Additive Model (GAM), p = 0.04)]. Coinfections were observed mainly between EPEC and Campylobacter spp. (10%). Antimicrobial resistance occurred in 0.6%, 29%, and 42% of DEC for ciprofloxacin, trimethoprim/sulfamethoxazole, and ampicillin, respectively.
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- 2019
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3. Carriage of two carbapenem-resistance genes in Pseudomonas aeruginosa isolated from hospital-acquired infections in children from Costa Rica: the importance of local epidemiology
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Valeria Peralta-Barquero, Christopher Mairena-Acuña, and Cristian Pérez-Corrales
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0301 basic medicine ,Microbiology (medical) ,Costa Rica ,medicine.medical_specialty ,030106 microbiology ,Case Report ,Infectious and parasitic diseases ,RC109-216 ,Drug resistance ,Microbial Sensitivity Tests ,medicine.disease_cause ,beta-Lactamases ,Microbiology ,03 medical and health sciences ,Medical microbiology ,Drug Resistance, Multiple, Bacterial ,Epidemiology ,Medicine ,Humans ,Carbapenem-Resistant Pseudomonas aeruginosa ,Pharmacology (medical) ,Pseudomonas Infections ,Allele ,Child ,Hospital-acquired infections ,Cross Infection ,biology ,Whole Genome Sequencing ,business.industry ,Pseudomonas aeruginosa ,Public Health, Environmental and Occupational Health ,biochemical phenomena, metabolism, and nutrition ,bacterial infections and mycoses ,biology.organism_classification ,030104 developmental biology ,Infectious Diseases ,Carriage ,Carbapenems ,Carbapenem resistance genes ,business ,Bacteria - Abstract
Background The assessment of Hospital-acquired infections due to multidrug-resistant bacteria involves the use of a variety of commercial and laboratory-developed tests to detect antimicrobial resistance genes in bacterial pathogens; however, few are evaluated for use in low- and middle-income countries. Methods We used whole-genome sequencing, rapid commercial molecular tests, laboratory-developed tests and routine culture testing. Results We identified the carriage of the metallo-β-lactamase blaVIM-2 and blaIMP-18 alleles in Carbapenem-Resistant Pseudomonas aeruginosa infections among children in Costa Rica. Conclusions The blaIMP-18 allele is not present in the most frequently used commercial tests; thus, it is possible that the circulation of this resistance gene may be underdiagnosed in Costa Rica.
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- 2021
4. SARS-CoV-2 Genomic Surveillance in Costa Rica: Evidence of a Divergent Population and an Increased Detection of a Spike T1117I Mutation
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Cristian Pérez-Corrales, Jose Arturo Molina-Mora, Coingesa-Cr Consorcio Interinstitucional de Estudios Genómicos del SARS-CoV Costa Rica, Melany Calderón, Estela Cordero-Laurent, Hebleen Brenes, Jan Felix Drexler, Adriana Godínez, Claudio Soto-Garita, Andres Moreira-Soto, Eugenia Corrales-Aguilar, and Francisco Duarte-Martínez
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Genetics ,education.field_of_study ,Mutation ,Lineage (genetic) ,Phylogenetic tree ,Population ,Outbreak ,Biology ,medicine.disease_cause ,Genome ,Pandemic ,medicine ,education ,Synonymous substitution - Abstract
Genome sequencing is a key strategy in the surveillance of SARS-CoV-2, the virus responsible for the COVID-19 pandemic. Latin America is the hardest hit region of the world, accumulating almost 20% of COVID-19 cases worldwide. Costa Rica was first exemplary for the region in its pandemic control, declaring a swift state of emergency on March 16th that led to a low quantity of cases, until measures were lifted in early May. From the first detected case in March 6th to December 31st almost 170 000 cases have been reported in Costa Rica, 99.5% of them from May onwards. We analyzed the genomic variability during the SARS-CoV-2 pandemic in Costa Rica using 185 sequences, 52 from the first months of the pandemic, and 133 from the current wave.Three GISAID clades (G, GH, and GR) and three PANGOLIN lineages (B.1, B.1.1, and B.1.291) are predominant, with phylogenetic relationships that are in line with the results of other Latin American countries, suggesting introduction and multiple re-introductions from other regions of the world. The whole-genome variant calling analysis identified a total of 283 distinct nucleotide variants. These correspond mostly to non-synonymous mutations (51.6%, 146) but 45.6% (129) corresponded to synonymous mutations. The 283 variants showed an expected power-law distribution: 190 single nucleotide mutations were identified in single sequences, only 16 single nucleotide mutations were found in >5% sequences, and only two mutations in >50% genomes. These mutations were distributed through the whole genome. However, 63.6% were present in ORF1ab, 11.7% in Spike gene and 10.6% in the Nucleocapsid gene. Additionally, the prevalence of worldwide-found variant D614G in the Spike (98.9% in Costa Rica), ORF8 L84S (1.1%) is similar to what is found elsewhere. Interestingly, the frequency of mutation T1117I in the Spike has increased during the current pandemic wave beginning in May 2020 in Costa Rica, reaching 29.2% detection in the full genome analyses in November 2020. This variant has been observed in less than 1% of the GISAID reported sequences worldwide in all the 2020. Structural modeling of the Spike protein with the T1117I mutation suggest a potential effect on the viral oligomerization needed for cell infection, but no differences with other genomes on transmissibility, severity nor vaccine effectiveness are predicted. Nevertheless, in-vitro experiments are required to support these in-silico findings. In conclusion, genome analyses of the SARS-CoV-2 sequences over the course of COVID-19 pandemic in Costa Rica suggest introduction of lineages from other countries as travel bans and measures were lifted, similar to results found in other studies, as well as an increase in the Spike-T1117I variant that needs to be monitored and studied in further analyses as part of the surveillance program during the pandemic.
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- 2020
5. Epidemiology, Etiology and Clinical Aspects of Childhood Acute Encephalitis in a Tertiary Pediatric Hospital in Costa Rica
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Sixto Bogantes-Ledezma, Alfonso Gutierrez-Mata, Adriana Yock-Corrales, Fabricio Sevilla-Acosta, Cristian Pérez-Corrales, and Kattia Camacho-Badilla
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Microbiology (medical) ,Costa Rica ,Male ,Pediatrics ,medicine.medical_specialty ,medicine.disease_cause ,Tertiary Care Centers ,03 medical and health sciences ,0302 clinical medicine ,030225 pediatrics ,Streptococcus pneumoniae ,Epidemiology ,medicine ,Enterovirus Infections ,Humans ,030212 general & internal medicine ,Prospective Studies ,Child ,Enterovirus ,Autoimmune encephalitis ,biology ,business.industry ,Mortality rate ,Infant ,Bacterial Infections ,medicine.disease ,biology.organism_classification ,Infectious Diseases ,Child, Preschool ,Pediatrics, Perinatology and Child Health ,Etiology ,Encephalitis ,Human herpesvirus 6 ,Female ,business - Abstract
BACKGROUND Limited data are available on childhood encephalitis in Latin America. Our study aimed to increase insight on clinical presentation, etiology and outcome of children with acute encephalitis in Costa Rica. METHODS We conducted a prospective, observational study during an 8-month period at the Hospital Nacional de Ninos "Dr. Carlos Saenz Herrera" in Costa Rica. Case definition was according to "International Encephalitis Consortium" in children
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- 2020
6. A 6-year retrospective study of clinical features, microbiology, and genomics of Shiga toxin-producing Escherichia coli in children who presented to a tertiary referral hospital in Costa Rica
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Cristian Perez-Corrales, Valeria Peralta-Barquero, Francisco Duarte-Martinez, and Gletty Oropeza-Barrios
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STEC ,Shiga-toxin Escherichia coli ,STEC genomic surveillance ,Microbiology ,QR1-502 - Abstract
ABSTRACTShiga-toxin-producing Escherichia coli (STEC) is associated with diarrhea and hemolytic uremic syndrome (HUS). STEC infections in Costa Rica are rarely reported in children. We gathered all the records of STEC infections in children documented at the National Children’s Hospital, a tertiary referral hospital, from 2015 to 2020. Clinical, microbiological, and genomic information were analyzed and summarized. A total of 3,768 diarrheal episodes were reviewed. Among them, 31 STEC were characterized (29 fecal, 1 urine, and 1 bloodstream infection). The prevalence of diarrheal disease due to STEC was estimated at 0.8% (n = 29/3,768), and HUS development was 6.4% (n = 2/31). The stx1 gene was found in 77% (n = 24/31) of STEC strains. In silico genomic predictions revealed a predominant prevalence of serotype O118/O152:H2, accompanied by a cluster exhibiting allele differences ranging from 33 to 8, using a core-genome multilocus sequence typing (cgMLST) approach. This is the first study using a genomic approach for STEC infections in Costa Rica.IMPORTANCEThis study provides a comprehensive description of clinical, microbiological, genomic, and demographic data from patients who attended the only pediatric hospital in Costa Rica with Shiga-toxin-producing Escherichia coli (STEC) infections. Despite the low prevalence of STEC infections, we found a predominant serotype O118/O152:H2, highlighting the pivotal role of genomics in understanding the epidemiology of public health threats such as STEC. Employing a genomic approach for this pathogen for the first time in Costa Rica, we identified a higher prevalence of STEC in children under 2 years old, especially those with gastrointestinal comorbidities, residing in densely populated regions. Limitations such as potential geographic bias and lack of strains due to direct molecular diagnostics are acknowledged, emphasizing the need for continued surveillance to uncover the true extent of circulating serotypes and potential outbreaks in Costa Rica.
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- 2024
- Full Text
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7. SARS-CoV-2 genomic surveillance in Costa Rica: Evidence of a divergent population and an increased detection of a spike T1117I mutation
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Claudio Soto-Garita, Melany Calderón-Osorno, Eugenia Corrales-Aguilar, Hebleen Brenes, Jan Felix Drexler, Adriana Godínez, Estela Cordero-Laurent, Coingesa-Cr Consorcio Interinstitucional de Estudios Genómicos del SARS-CoV Costa Rica, Francisco Duarte-Martínez, Jose Arturo Molina-Mora, Andres Moreira-Soto, and Cristian Pérez-Corrales
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0301 basic medicine ,Microbiology (medical) ,Costa Rica ,Male ,Models, Molecular ,Protein Conformation ,030106 microbiology ,Population ,Genomics ,Biology ,medicine.disease_cause ,Microbiology ,Genome ,DNA sequencing ,Virus ,03 medical and health sciences ,Genomic surveillance ,Pandemic ,Genetic variation ,Genetics ,medicine ,Humans ,COSTA RICA ,education ,Molecular Biology ,Ecology, Evolution, Behavior and Systematics ,Phylogeny ,education.field_of_study ,Mutation ,SARS-CoV-2 ,COVID-19 ,Genetic Variation ,030104 developmental biology ,Infectious Diseases ,Population Surveillance ,Spike Glycoprotein, Coronavirus ,Female ,Research Paper - Abstract
Genome sequencing is a key strategy in the surveillance of SARS-CoV-2, the virus responsible for the COVID-19 pandemic. Latin America is the hardest-hit region of the world, accumulating almost 20% of COVID-19 cases worldwide. In Costa Rica, from the first detected case on March 6th to December 31st almost 170,000 cases have been reported. We analyzed the genomic variability during the SARS-CoV-2 pandemic in Costa Rica using 185 sequences, 52 from the first months of the pandemic, and 133 from the current wave. Three GISAID clades (G, GH, and GR) and three PANGOLIN lineages (B.1, B.1.1, and B.1.291) were predominant, suggesting multiple re-introductions from other regions. The whole-genome variant calling analysis identified a total of 283 distinct nucleotide variants, following a power-law distribution with 190 single nucleotide mutations in a single sequence, and only 16 mutations were found in >5% sequences. These mutations were distributed through the whole genome. The prevalence of worldwide-found variant D614G in the Spike (98.9% in Costa Rica), ORF8 L84S (1.1%) is similar to what is found elsewhere. Interestingly, the frequency of mutation T1117I in the Spike has increased during the current pandemic wave beginning in May 2020 in Costa Rica, reaching 29.2% detection in the full genome analyses in November 2020. This variant has been observed in less than 1% of the GISAID reported sequences worldwide in 2020. Structural modeling of the Spike protein with the T1117I mutation suggests a potential effect on the viral oligomerization needed for cell infection, but no differences with other genomes on transmissibility, severity nor vaccine effectiveness are predicted. In conclusion, genome analyses of the SARS-CoV-2 sequences over the course of the COVID-19 pandemic in Costa Rica suggest the introduction of lineages from other countries and the detection of mutations in line with other studies, but pointing out the local increase in the detection of Spike-T1117I variant. The genomic features of this virus need to be monitored and studied in further analyses as part of the surveillance program during the pandemic., Graphical abstract Unlabelled Image
- Published
- 2021
8. Virulence Factor Genes in Staphylococcus aureus Isolated From Diabetic Foot Soft Tissue and Bone Infections
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Benjamin A. Lipsky, Javier Aragón-Sánchez, Gerardo Víquez-Molina, Cristian Pérez-Corrales, María Eugenia López-Valverde, and Christian Murillo-Vargas
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0301 basic medicine ,DNA, Bacterial ,Male ,Staphylococcus aureus ,medicine.drug_class ,Virulence Factors ,030106 microbiology ,Antibiotics ,Virulence ,030209 endocrinology & metabolism ,medicine.disease_cause ,Polymerase Chain Reaction ,Microbiology ,Bone Infection ,03 medical and health sciences ,0302 clinical medicine ,medicine ,Humans ,Prospective Studies ,business.industry ,SCCmec ,Osteomyelitis ,Soft Tissue Infections ,Soft tissue ,General Medicine ,Middle Aged ,Staphylococcal Infections ,bacterial infections and mycoses ,medicine.disease ,Diabetic foot ,Diabetic Foot ,Cross-Sectional Studies ,Surgery ,Female ,business ,Follow-Up Studies - Abstract
The aim of this study is to describe the presence of genes encoding for 4 virulence factors (pvl, eta, etb, and tsst), as well as the mecA gene conferring resistance to beta-lactam antibiotics, in patients with diabetes and a staphylococcal foot infection. We have also analyzed whether isolates of Staphylococcus aureus from bone infections have a different profile for these genes compared with those from exclusively soft tissue infections. In this cross-sectional study of a prospectively recruited series of patients admitted to the Diabetic Foot Unit, San Juan de Dios Hospital, San José, Costa Rica with a moderate or severe diabetic foot infection (DFI), we collected samples from infected soft tissue and from bone during debridement. During the study period (June 1, 2014 to May 31, 2016), we treated 379 patients for a DFI. S aureus was isolated from 101 wound samples, of which 43 were polymicrobial infections; we only included the 58 infections that were monomicrobial S aureus for this study. Infections were exclusively soft tissue in 17 patients (29.3%) while 41 (70.7%) had bone involvement (osteomyelitis). The mecA gene was detected in 35 cases (60.3%), pvl gene in 4 cases (6.9%), and tsst gene in 3 (5.2%). We did not detect etA and etB in any of the cases. There were no differences in the profile of S aureus genes encoding for virulence factors (pvl, etA, etB, and tsst) recovered from DFIs between those with just soft tissue compared to those with osteomyelitis. However, we found a significantly higher prevalence of pvl+ strains of S aureus associated with soft tissue compared with bone infections. Furthermore, we observed a significantly longer time to healing among patients infected with mecA+ (methicillin-resistant) S aureus (MRSA).
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- 2018
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