441 results on '"Cryptosporidium spp"'
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2. A molecular survey of zoonotic pathogens of public health importance in rodents/shrews and their ectoparasites trapped in Puducherry, India.
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Eikenbary, Brenna, Devaraju, Panneer, Chakkravarthi, Aravindasamy, Sihag, Krishan Kumar, Nathan, Terence, Thangaraj, Gowdham, Srinivasan, Lakshmy, and Kumar, Ashwani
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COXIELLA burnetii ,METHICILLIN-resistant staphylococcus aureus ,Q fever ,TSUTSUGAMUSHI disease ,ZOONOSES ,LEPTOSPIRA interrogans ,MITES - Abstract
Background Globally, India has a high zoonotic disease burden and lacks surveillance data in humans and animals. Rodents are known reservoirs for many zoonotic diseases and their synanthropic behavior poses a great public health threat. Methods In this study, trapped rodents/shrews from randomly selected villages within Puducherry, India, and their ectoparasites were screened for zoonotic pathogens, namely, Orientia tsutsugamushi , other pathogenic rickettsiae, Leptospira spp. Cryptosporidium spp. Coxiella burnetii and methicillin-resistant Staphylococcus aureus (MRSA) using conventional PCR. A total of 58 rodents/shrews were trapped from 11 villages. The species trapped were Suncus murinus (49/58, 84.48%), Rattus rattus (8/58, 13.79%) and Rattus norvegicus (1/58, 1.72%). All ectoparasites collected were identified as mites and its infestation rate was 46.55% (27/58). Results Real-time PCR targeting the 47 kDa gene of O. tsutsugamushi revealed positivity in one rodent and one shrew (3.45%) and two mite pools (7.41%). Conventional PCR targeting the 56 kDa gene revealed positivity in one shrew and two mite pools and the phylogenetic analysis of all three amplicons indicated the circulation of the Gilliam-related serotype. MRSA was detected in the alimentary tract of a shrew (1/32, 3.13%). Leptospira spp. Rickettsia, Cryptosporidium spp. and Co. burnetii tested negative. Conclusions The detection of zoonotic pathogens within reservoir hosts and vectors poses a risk of transmission to humans. This study signifies the need for zoonotic pathogen surveillance in synanthropic rodents/shrews. [ABSTRACT FROM AUTHOR] more...
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- 2024
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3. The spectrum of parasitic infections with emphasis on the clinico-epidemiological characteristics and risk factors among immunocompromised and immunocompetent patients at a university hospital in Northern India.
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Kar, Mitra, Singh, Romya, Tejan, Nidhi, Sahu, Chinmoy, Tiwari, Ritika, Jain, Mudra, Kumar, Awadhesh, Patel, Sangram S., Goyal, Urvashi, and Ghoshal, Ujjala
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Introduction: Intestinal parasitic infections pose a substantial threat to public health and are a huge burden to the economic development of a developing country. We aimed to identify the spectrum of intestinal parasitic infections with an emphasis on demographic and clinical characteristics observed among immunocompromised and immunocompetent patients. Materials and Methods: This observational study was performed in the Parasitology section of the Department of Microbiology from January 2022 to July 2022. A total of 2628 stool samples were obtained from patients presenting with chief complaints of abdominal pain, distension, vomiting, and foul-smelling feces. All the clinical and diagnostic data of the patients enrolled in the above-mentioned period were extracted from the ward files, hospital electronic records, and laboratory registers. Result: A total of 2628 stool samples were sent to the Parasitology section of the Department of Microbiology. Out of the above-mentioned samples, 70 (70/2628, 2.66%) samples yielded gastrointestinal parasites on microscopic examination. The mean age of the patients included in our cohort study was 32.53 ± 16.21 years with a male predominance of 72.86% (51/70, 72.86%). The most common gastrointestinal parasite identified from stool samples was Giardia lamblia (61/70, 87.14%). All cases of opportunistic gastrointestinal infection caused by Cryptosporidium spp. (4/70, 5.71%) in our study cohort were found to infest the immunocompromised patients. Conclusion: This study determines the spectrum of intestinal parasitic infections among the immunocompromised and immunocompetent individuals and guides physicians in starting appropriate anti-parasitic treatment along with the instillation of strict hand hygiene techniques. [ABSTRACT FROM AUTHOR] more...
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- 2024
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4. Dairy farm management practices as risk factors linked to Cryptosporidium spp. infection in dairy calves
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Agrawal, R., Shukla, P.C., Pande, N., and Shreen
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- 2023
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5. Prevalencia de Cryptosporidium spp., Cystoisospora belli y Cyclospora cayetanensis en pacientes infectados con VIH en el municipio de Veracruz, México.
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Zaldívar-López, Alejandra, Hernández-Romano, Pablo A., Infanzón-Ruiz, Raúl, Bravo-Sarmiento, Elidé, Torres-Hernández, Rosa M., and López-Balderas, Nayali A.
- Abstract
Introduction. The human immunodeficiency virus (HIV) infects and gradually destroy CD4+ cells of the immune system and eventually leading to immunodeficiency. Gastrointestinal opportunistic parasitosis are a frequent cause of morbidity and mortality in HIV+ patients. Objective. To determine the prevalence of the opportunistic parasites Cryptosporidium spp., Cystoisospora belli and Cyclospora cayetanensis, in patients infected with HIV in the municipality of Veracruz. Mexico. Material and methods. 230 non-serial stool samples from patients with HIV were collected. The smears were analysed by direct method and modified Ziehl-Neelsen staining. Results. A prevalence of parasitism of 92.17% was found, 85.22% of Cryptosporidium spp., and C. cayetanensis were identified as mono-parasitism or in association with each other and with other nonopportunistic parasites. Cryptosporidium spp. was the most prevalent parasite (72.60%), followed by C. cayetanensis (57.78%), Endolimax nana (36.52%), Blastocystis spp. (17.83%) and Entamoeba Complex (7 .83%). No samples with Cystoisospora belli were identified. Conclusions. Compared to the only report of opportunistic parasites in the HIV+ population of the Veracruz municipality, there was an increase in the prevalence of Cryptosporidium spp. and a abscense of C. belli. Even with a greater access to antiretroviral treatment, in Mexico, parasitosis continue to have high prevalences. Therefore, routine monitoring of opportunistic parasitism is necessary, using appropriate techniques to avoid underdiagnosis and the risk of comorbidity and mortality that they represent in the HIV+ population. [ABSTRACT FROM AUTHOR] more...
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- 2023
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6. Il controllo integrato dei principali parassiti gastrointestinali dei piccoli ruminanti.
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Perrucci, Stefania
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Copyright of Summa, Animali da Reddito is the property of Point Veterinaire Italie s.r.l. and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) more...
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- 2023
7. Molecular analysis of Cryptosporidium species in domestic goat in central Iraq
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Akeel M. Al-Musawi, Abdul Hussein H. Awad, and Mansoor J. Alkhaled
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cryptosporidium spp ,genotype ,subtypes ,domestic goat ,iraq ,Veterinary medicine ,SF600-1100 - Abstract
Cryptosporidium spp. is a significant parasitic disease that results in diarrhea and gastroenteritis in humans and animals worldwide. The present study aimed to investigate the molecular diversity of Cryptosporidium species in domestic goats. A total of a hundred feces samples were collected from four locations in Babylon city in central Iraq. All the samples were investigated phenotypically using a modified Ziehl-Neelsen stain method and genotypically using conventional and nested PCR methods based on a partial sequence of heat shock protein 70 (Hsp70) and 60 kDa glycoprotein (gp60) genes, and finally, phylogenetic analysis method. The molecular results showed five species of Cryptosporidium, including C. parvum, C. hominis, C. ryana, C. xiaoi, and C. bovis. The phylogenetic results of partial sequence of gp60 gene for C. parvum and C. hominis isolate two subtypes were established IIdA21G1 and IIdA19G1 belong to C. parvum. For C. hominis, three subtypes were detected: IbA21G2, IbA13G3, and IbA19G2. This study showed that Cryptosporidium parvum (zoonotic) is more prevalent than other Cryptosporidium species in goats from this area. This suggests that zoonotic transmission is the primary mode of transmission of Cryptosporidium infection in Babylon province. more...
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- 2022
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8. Seasonal distribution of Cryptosporidium spp., Giardia duodenalis and Enterocytozoon bieneusi in Tibetan sheep in Qinghai, China
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Fan Yang, Li Ma, Jing-min Gou, Hui-zhong Yao, Mei Ren, Bing-ke Yang, and Qing Lin
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Cryptosporidium spp ,Giardia duodenalis ,Enterocytozoon bieneusi ,Tibetan sheep ,China ,Infectious and parasitic diseases ,RC109-216 - Abstract
Abstract Background Cryptosporidium spp., Giardia duodenalis and Enterocytozoon bieneusi can cause important intestinal diseases in ruminants. However, data on the distribution of these three protozoan pathogens in Tibetan sheep are limited. Methods We collected 761 fecal samples from Tibetan sheep across four seasons in Qinghai Province, China, and screened the samples for Cryptosporidium spp., G. duodenalis and E. bieneusi using PCR-based sequence analysis of the genes encoding 18S ribosomal RNA, triosephosphate isomerase and the internal transcribed spacer, respectively. Results The positivity rates of Cryptosporidium spp., G. duodenalis and E. bieneusi in Tibetan sheep were 3.68% (28/761 samples), 1.58% (12/761) and 6.44% (49/761), respectively. Four species of Cryptosporidium were identified: C. xiaoi (n = 13 samples), C. ubiquitum (n = 8), C. bovis (n = 6) and C. ryanae (n = 1). Two G. duodenalis assemblages, namely the A (n = 2 samples) and E (n = 10) assemblages, were detected. Five zoonotic E. bieneusi genotypes were found: BEB6 (n = 21 samples), COS-I (n = 14), CHS3 (n = 11) and CGS1 (n = 2) from group 2, and PIGEBITS5 (n = 1) from group 1. Geographic differences in the distribution of E. bieneusi, and seasonal differences for all the three protozoan pathogens were noted. Conclusions Our results elucidate the prevalence and genetic diversity of these three pathogens in Tibetan sheep across different regions and seasons, including zoonotic pathogens such as C. ubiquitum, C. ryanae, G. duodenalis assemblage A and five genotypes of E. bieneusi. Graphical Abstract more...
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- 2022
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9. ELISA–based Point Prevalence of enteropathogens in diarrheic calves in Central Anatolia Region of Turkey.
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Işik–Uslu, Nermin, Derinbay–Ekic, Ozlem, and Avci, Oğuzhan
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CRYPTOSPORIDIUM ,ENZYME-linked immunosorbent assay - Abstract
Copyright of Revista Cientifica de la Facultade de Veterinaria is the property of Universidad del Zulia, Facultad de Ciencias Veterinarias and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) more...
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- 2023
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10. Cryptosporidium spp. prevalence in the general population in Guinea: first large-scale screening study
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Guilavogui Timothé, Gantois Nausicaa, Desramaut Jérémy, Cissé Fode Ibrahima, Touré Salif Cherif, Kourouma Bakary Luther, Preda Cristian, Chabé Magali, Viscogliosi Eric, and Certad Gabriela
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cryptosporidium spp ,molecular epidemiology ,transmission ,zoonosis ,guinea ,africa ,Infectious and parasitic diseases ,RC109-216 - Abstract
Cryptosporidium is a leading cause of diarrheal mortality in children in Africa and Asia. Despite the public health significance of this parasite, its molecular epidemiology and circulation in Guinea remain poorly understood. Therefore, this study aimed to determine the prevalence and genotype distribution of Cryptosporidium in the Guinean general population. To achieve this, fecal samples were collected from 834 individuals, both with and without digestive disorders, at two hospitals in Conakry. The presence of the parasite in the stool samples was detected using nested PCR targeting the SSU rDNA gene, followed by sequencing of the PCR products for genotyping of the isolates. The PCR-based prevalence was 0.12% for the whole cohort, and 0.2% among adults. The low frequency of Cryptosporidium observed in the current study is thus consistent with the prevalence of this parasite already reported in certain other African countries. The species identified in the positive samples was Cryptosporidium hominis. This study is the first to report the prevalence of Cryptosporidium in the general population of Guinea. Given the potential of this parasite to cause life-threatening diarrhea, further studies are needed to clarify the epidemiology of Cryptosporidium in this country. more...
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- 2024
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11. Methods used for concentrating oocysts of Cryptosporidium spp., spores Encephalitozoon spp. and Enterocytozoon spp. and their occurrence in Slovak water samples
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Monika Sučik and Alexandra Valenčáková
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Cryptosporidium spp ,Encephalitozoon spp ,Enterocytozoon spp ,filtration ,PCR ,Environmental sciences ,GE1-350 - Abstract
The number of outbreaks of water-borne diseases caused by parasites seems to have increased in recent years. Nevertheless, the occurrence of these pathogens in water generally pays little attention. Waterborne transmission is a major route in the epidemiology of the parasite and therefore poses a serious public health problem. Cryptosporidium spp., Encephalitozoon spp. and Enterocytozoon spp. parasites are recognised worldwide as a common cause of diarrhoea. In most cases, it is a dilapidated or poorly maintained standard sanitation and water supply. It is important to perform periodic tests on protozoa, which are often lacking in small laboratories. Since it is necessary to filter large volumes of water for reliable diagnostics and consequently, it is difficult to concentrate them in a large volume of filtrate, it is not easy to detect their presence in the water. Various filtration methods are used to filter these pathogens from water, but cryptosporidial oocysts and microsporidia spores still occur in most of the world’s and Slovak recreational waters. Therefore, it would be appropriate to use the abilities of gill-breathing aquatic animals that filter cryptosporidial oocysts and microsporidia spores from the water by absorbing them with food. Zeolite can also purify water by capturing high concentrations of contaminants, including cryptosporidial oocysts and microsporidial spores. more...
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- 2023
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12. Seasonal distribution of Cryptosporidium spp., Giardia duodenalis and Enterocytozoon bieneusi in Tibetan sheep in Qinghai, China.
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Yang, Fan, Ma, Li, Gou, Jing-min, Yao, Hui-zhong, Ren, Mei, Yang, Bing-ke, and Lin, Qing
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CRYPTOSPORIDIUM , *SHEEP , *TRIOSE-phosphate isomerase , *GIARDIA , *RIBOSOMAL DNA , *RIBOSOMAL RNA , *INTESTINAL diseases , *ENTEROCYTOZOON bieneusi - Abstract
Background: Cryptosporidium spp., Giardia duodenalis and Enterocytozoon bieneusi can cause important intestinal diseases in ruminants. However, data on the distribution of these three protozoan pathogens in Tibetan sheep are limited. Methods: We collected 761 fecal samples from Tibetan sheep across four seasons in Qinghai Province, China, and screened the samples for Cryptosporidium spp., G. duodenalis and E. bieneusi using PCR-based sequence analysis of the genes encoding 18S ribosomal RNA, triosephosphate isomerase and the internal transcribed spacer, respectively. Results: The positivity rates of Cryptosporidium spp., G. duodenalis and E. bieneusi in Tibetan sheep were 3.68% (28/761 samples), 1.58% (12/761) and 6.44% (49/761), respectively. Four species of Cryptosporidium were identified: C. xiaoi (n = 13 samples), C. ubiquitum (n = 8), C. bovis (n = 6) and C. ryanae (n = 1). Two G. duodenalis assemblages, namely the A (n = 2 samples) and E (n = 10) assemblages, were detected. Five zoonotic E. bieneusi genotypes were found: BEB6 (n = 21 samples), COS-I (n = 14), CHS3 (n = 11) and CGS1 (n = 2) from group 2, and PIGEBITS5 (n = 1) from group 1. Geographic differences in the distribution of E. bieneusi, and seasonal differences for all the three protozoan pathogens were noted. Conclusions: Our results elucidate the prevalence and genetic diversity of these three pathogens in Tibetan sheep across different regions and seasons, including zoonotic pathogens such as C. ubiquitum, C. ryanae, G. duodenalis assemblage A and five genotypes of E. bieneusi. [ABSTRACT FROM AUTHOR] more...
- Published
- 2022
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13. Prevalence and genotypic characterization of zoonotic intestinal protozoan parasites in transboundary migratory Mongolian Gazelles ( Procapra gutturosa ): the first survey.
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Gao S, Hu B, Li G, An X, Xing Y, Han S, Chun Y, Han L, Xi D, Li W, and He H
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The migration of Mongolian gazelles ( Procapra gutturosa ) poses a potential risk of outbreak for zoonotic intestinal protozoan parasite infections. This study aims to investigate the infection status of zoonotic intestinal protozoan parasites in these migratory Mongolian gazelles. We collected 120 fecal samples from Mongolian gazelles during their migration from Mongolia to China in December 2023. These samples were analysed using amplification and sequencing of partial SSU rRNA genes to detect the 4 presence of zoonotic intestinal protozoan parasites and characterize their genotypes. Our analysis revealed the presence of several zoonotic intestinal protozoan parasites in the sampled Mongolian gazelles. Cryptosporidium spp. was detected in 14.17% (17/120) of the samples, followed by Cystoisospora belli in 13.33% (16/120), Blastocystis sp. in 16.67% (20/120) and Cyclospora cayetanensis in 30.00% (36/120). Moreover, we identified novel host-adapted genotypes of Cryptosporidium spp. and C. belli , as well as the presence of ST2 and ST13 Blastocystis sp. subtypes, while distinct genotypes were found in Blastocystis sp. and C. cayetanensis . This study revealed the status of 4 prevalent zoonotic intestinal protozoan parasite infections in Mongolian gazelles and provided crucial insights into their characteristics. The prevalence of these parasites in the population highlights the potential risk of cross-border transmission of infectious diseases associated with long-distance migration. Furthermore, the identification of novel genotypes contributes to our understanding of the genetic diversity and adaptation of these parasites. These findings can inform the development of protective measures to mitigate the impact of these infections on the health and survival of Mongolian gazelles. more...
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- 2024
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14. High prevalence of Cryptosporidium infection in Iranian patients suffering from colorectal cancer
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Koroush Ghanadi, Amal Khudair Khalaf, Ata Jafrasteh, Khatereh Anbari, and Hossein Mahmoudvand
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Colorectal cancer ,Parasite ,Cryptosporidium spp ,Iran ,Diarrhea ,Infectious and parasitic diseases ,RC109-216 - Abstract
The present investigation was designed to study the prevalence of cryptosporidiosis in the colorectal cancer patients compared to the healthy subjects. The present descriptive case-control study was performed on 174 subjects including 87 healthy people and 87 patients with colorectal cancer attending to general hospitals in Lorestan Province, Western Iran, during October 2019–August 2020. A fresh stool specimen was collected from each subject in a sterile labeled container. The collected stool samples were concentrated using the sucrose flotation method and then prepared for Ziehl-Neelsen staining for microscopic examination. All samples were also tested using the Nested-PCR assays by amplifying the 18S rRNA gene for the presence of Cryptosporidium DNA. Demographic and possible risk factors such as age, gender, residence, agriculture activity, history of contact with livestock, consumption unwashed fruits/vegetables, and hand washing before eating were investigated in all the studied subjects using a questionnaire. Of the 87 patients with colorectal cancer, 37 (42.5%) had Cryptosporidium infection. A significant difference (p more...
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- 2022
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15. INVESTIGATION OF THE FREQUENCY OF CRYPTOSPORIDIUM SPP. AND GIARDIA INTESTINALIS IN PATIENTS WITH SUPPRESSED IMMUNE SYSTEM.
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Bayraktar, Mehmet, Cömert, Medine, and Öztürk, Ali
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CRYPTOSPORIDIUM ,GIARDIA ,IMMUNE system ,DIARRHEA ,IMMUNOCOMPROMISED patients - Abstract
Copyright of Journal of Health Sciences of Kocaeli University / Kocaeli Üniversitesi Sağlık Bilimleri Dergisi is the property of Institute of Health Sciences of Kocaeli University and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) more...
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- 2022
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16. New record of parasitic infection among school children of Lower Dir Pakistan
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Y. A. J. Fadladdin, H. Ur. Rahman, and M. Kabir
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new records ,Cryptosporidium spp ,Schistosoma spp ,Toxocara spp ,urban areas ,Science ,Biology (General) ,QH301-705.5 ,Zoology ,QL1-991 ,Botany ,QK1-989 - Abstract
Abstract Intestinal parasitic infections (IPIs) are a major cause of morbidity worldwide and have been described as an important public health problem. The present study aimed to determine the un usual parasitic infection and identification of risk factors among 4-12years old school age children residing in lower dir district, Pakistan from 2018- 2019. Of the 400 children studied in which the overall prevalence rate for intestinal parasitic infections was found to be 71.75%. Children infected with single parasite accounted for 67.94% and 32.05% were detected with poly-parasitism. Shistosoma japonicum (0.69%), Taxocara spp (0.69%) and cryptosporidium (0.69%), were identified in children living in studied areas. We conclude that there is a need for mass scale campaigns to create awareness regarding health and hygiene in children and the need for development of effective poverty control programmes. more...
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- 2022
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17. Evaluation of pathogen risks using QMRA to explore wastewater reuse options: A case study from New Delhi in India
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Rajashree Hajare, Pawan Labhasetwar, and Pranav Nagarnaik
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cryptosporidium spp ,giardia spp ,health risks ,pathogens ,quantitative microbial risk assessment (qmra) ,wastewater reuse ,Environmental technology. Sanitary engineering ,TD1-1066 - Abstract
Selecting appropriate reuse for treated wastewater is a challenge. The current investigation outlines the utilization of quantitative microbial risk assessment (QMRA) to assist Effluent Treatment Plant (ETP) management to determine the best-possible reuse of treated wastewater from 11 ETPs in Delhi. Four representative pathogens: pathogenic Escherichia coli spp., Salmonella spp., Cryptosporidium spp. and Giardia spp. were selected to characterize microbial water quality. Reuse options selected based on the survey and interaction with ETP managers include crop irrigation, garden irrigation, toilet flush and industrial applications. The probability of infection was characterized for two exposure groups: workers and children. Water quality monitoring indicates the occurrence of pathogenic E. coli spp. (100%), Salmonella spp. (63%), Cryptosporidium spp. (81%) and Giardia spp. (45%) in the treated wastewater. QMRA reveals the annual median-probability of infection above acceptable limits for pathogenic E. coli spp., Cryptosporidium spp. and Salmonella spp. The probabilities of Giardia-associated infections were low. Adults showed a 1.24 times higher probability of infection compared to children. Sensitivity analysis indicated pathogen concentration as the most critical factor. The study highlights that the existing plans for chlorination-based treatment technology may prove insufficient in reducing the risk for selected reuse options; but, alternate on-site control measures and up-grading water reuse protocol may be effective. more...
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- 2021
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18. Molecular characterization of Cryptosporidium spp. obtained from fecal samples of immunosuppressed patients from Brazil
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Flávia de Souza Cunha, Higor Wilson Jann, Jocemir Ronaldo Lugon, José Mauro Peralta, and Regina Helena Saramago Peralta
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Cryptosporidium spp ,Immunosuppressed populations ,Kidney transplant ,Genetic diversity ,Arctic medicine. Tropical medicine ,RC955-962 - Abstract
Abstract Background: Cryptosporidium spp. are pathogenic protozoans that play an important role in developing diseases in the elderly, children, and immunosuppressed individuals. Methods: The objective of this study was to detect and genetically characterize Cryptosporidium spp. in kidney transplanted patients (n = 97 samples; group 1) and immunosuppressed individuals from an outpatient clinic suspected of having Cryptosporidium infection (n = 53 samples; group 2). All fecal samples were analyzed by parasitological stool examination, immunochromatographic test, and real-time polymerase chain reaction (real-time PCR). Cryptosporidium-positive samples were tested using nested PCR for the gp60 gene, followed by sequencing for subtype determination. Results: Parasitological examination was negative in all Group 1, and positive in four Group 2 samples. Real-time PCR revealed Cryptosporidium in 13 samples: four in Group 1 (three C. hominis and one C. parvum) and nine in Group 2 (seven C. hominis, one C. parvum, and one mixed C. hominis/C. parvum). The immunochromatographic test was reactive in 11 samples (four in Group 1 and seven in Group 2). All 11 C. hominis isolates were identified as subtype IbA10G2 and one C. parvum as subtype IIbA15G2R1. All C. hominis belonged to subtype IbA10G2, which is recognized as the most prevalent and pathogenic subtype. Conclusions: This study showed, for the first time, that the presence of Cryptosporidium subtypes is considered more virulent in Brazilian transplanted kidney patients. more...
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- 2022
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19. Molecular characterization of Cryptosporidium spp., Enterocytozoon bieneusi and Giardia duodenalis in laboratory rodents in China.
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Wang, Nanhao, Wang, Ke, Liu, Yufeng, Zhang, Xiaotian, Zhao, Jinfeng, Zhang, Sumei, and Zhang, Longxian
- Abstract
Copyright of Parasite (1252607X) is the property of EDP Sciences and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) more...
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- 2022
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20. New record of parasitic infection among school children of Lower Dir Pakistan.
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Fadladdin, Y. A. J., Ur. Rahman, H., and Kabir, M.
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PARASITIC diseases ,SCHOOL children ,INTESTINAL infections ,CHILD development - Abstract
Copyright of Brazilian Journal of Biology is the property of Instituto Internacional de Ecologia and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) more...
- Published
- 2022
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21. Cryptosporidium parvum – zoonotic subtype IIdA15G1 in a Slovakian patient
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Kristína Mravcová, Gabriela Štrkolcová, Rastislav Mucha, Eva Barbušinová, Mária Goldová, Jana Kačírová, and Marián Maďar
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slovakia ,nested pcr ,cryptosporidium spp ,zoonotic subtype ,veterinary student ,Agriculture ,Environmental sciences ,GE1-350 - Abstract
Introduction and objectives The parasite Cryptosporidium spp. is an intracellular protozoa which has a broad range of hosts and zoonotic potential. It presents a serious health risk for agricultural workers and veterinarians. The aim of the study was to identify the species and subtypes of Cryptosporidium occurring in a veterinary student who came into contact with calves on a farm. Material and Methods The Ziehl-Neelsen staining technique was employed to confirm the presence of Cryptosporidium oocysts. ELISA test was applied to detect coproantigen in faecal specimens. Nested PCR was used to amplify a small ribosomal subunit (SSU rRNA) and sequencing of the GP60 gene served to identify the zoonotic subtypes. Results The nested PCR allowed to confirm the C. parvum species; subsequently, the IIdA15G1 zoonotic subtype was identified. Conclusions This is the first confirmed case in Slovakia of human cryptosporidiosis caused by the unique subtype IIdA15G1. more...
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- 2020
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22. Potential sources of infection with selected zoonotic agents in the veterinary work environment – pilot studies
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Angelina Wójcik-Fatla, Jacek Sroka, Violetta Zając, Anna Sawczyn-Domańska, Anna Kloc, Jacek Zwoliński, Teresa Kłapeć, Maria Bernadeta Studzińska, Robert Chmura, and Jacek Dutkiewicz
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environment ,coxiella burnetii ,toxoplasma gondii ,veterinarians ,leptospira spp ,cryptosporidium spp ,giardia duodenalis ,Agriculture ,Environmental sciences ,GE1-350 - Abstract
Introduction and Objective The problem of occupational biohazards is very important, especially in the field of agriculture and in human and veterinary medicine. The aim of the study was to determine the potential sources of infection in veterinary professionals with selected zoonotic agents, including: Toxoplasma gondii , Giardia duodenalis , Leptospira spp., Cryptosporidium spp. and Coxiella burnetii . Material and methods A total of 50 air samples from barns, piggeries and veterinary surgeries were examined for the presence of Leptospira spp. and C. burnetii DNA. Serum samples of 86 pigs and 80 cows were tested for the presence of antibodies to Leptospira spp. and to phase I and II C. burnetii antigens. Serum of 70 cats were tested for the presence of antibodies to T. gondii and 65 samples of cat faeces for the presence of T. gondii oocysts. The presence of G. duodenalis and Cryptosporidium spp. were examined in 50 of dog faeces and 50 of bovine faeces samples. Results DNA of Leptospira spp. was detected in 2 air samples from the piggeries (4%). C. burnetii DNA was not found in any sample. Anti- Leptospira spp. antibodies were detected in 51 (59.3%) of examined pigs. Neither anti- Leptospira spp. nor anti- C. burnetii antibodies were found among samples of bovine serum. Anti- T. gondii antibodies was found in 52 cat serum samples (74.3%). Among samples of cat faeces, no T. gondii oocysts were detected. In one sample of cattle stool (2%), G. duodenalis was detected and in another (2%) – Cryptosporidium spp. G. duodenalis was detected in 7 samples (14%) and Cryptosporidium spp. in 2 samples (2%) of dog faeces. Conclusions The results of this study demonstrate the potential risk of infection with Leptospira spp. in veterinarians working with pigs. Veterinarians could be also be at risk of infection with T. gondii and G. duodenalis . more...
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- 2020
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23. Prevalence of Cryptosporidium spp. infection in rodents and chickens in Franceville, Gabon.
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Makouloutou-Nzassi P, Bouchedi B, Mangombi-Pambou JB, Longo-Pendy NM, N'dilimabaka N, Bangueboussa F, Koumba S, Matoumba AM, Boundenga L, Maganga GD, and Mintsa-Nguema R
- Abstract
Background and Aim: Cryptosporidium spp. members of the phylum Apicomplexa are obligate protozoan parasites capable of infecting various vertebrate hosts, including rodents and chickens. Infection caused by these parasites may lead to zoonotic diseases in humans. The aim of this study was to estimate the prevalence of Cryptosporidium spp. in rodents and domestic chickens sampled in Franceville, Gabon., Materials and Methods: Two hundred and eighty-five samples were collected, of which 185 samples were from rodents and 100 from domestic chickens. Microscopy after modified Ziehl-Neelsen staining and nested polymerase chain reaction targeting the small subunit (SSU) rRNA gene were used to examine Cryptosporidium spp., Results: The overall prevalence of Cryptosporidium oocysts was 55.8%, with a prevalence of 72.4% in rodents and 25.0% in domestic chickens. Molecular analysis showed that Cryptosporidium spp. were present in 4.0% of the samples. No significant correlation was observed between Cryptosporidium spp. carriage and sex or location in this study. These results indicate that Cryptosporidium spp. persist and circulate in the studied animal species in Franceville, Gabon., Conclusion: Infection with Cryptosporidium is very common in rodents and chickens in Franceville. The potential risk of human contamination cannot be ruled out. More research should be conducted to characterize Cryptosporidium species circulating in rodents and chickens in Gabon. Such studies are essential to better understand the epidemiology of this protozoan and its potential impact on public health., Competing Interests: The authors declare that they have no competing interests., (Copyright: © Makouloutou-Nzassi, et al.) more...
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- 2024
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24. In Pediatric Gastroenteritis: the Presence of Helicobacter Pylori Prevalence of Entamoeba Histolytica, Giardia Intestinalis And Cryptosporidium Spp.
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Çelebi, Demet and Çelebi, Özgür
- Subjects
- *
GASTROENTERITIS , *HELICOBACTER pylori , *GIARDIA lamblia - Abstract
Objective: Parasitic gastroenteritis continues to be an important cause of morbidity and mortality in children worldwide. The purpose of this study is to evaluate the frequency of Entamoeba histolytica, Giardia intestinalis and Cryptosporidium spp species in diarrhea samples taken from children. Methods: Between August 2018 and January 2019, 400 stool samples of children suffering from gastroenteritis were examined for important intestinal protozoa agents by both direct examination (Native & Lugol iodine) and antigen detection tests. Results: It was determined that G. intestinalis was the most common pathogen (13%). This was followed by Cryptosporidium spp (7.75), E. histolytica (4.00%), T. hominis (1.00%). The common complaint in children in this study was diarrhea. Conclusion: E. histolytica, G. intestinalis and Cryptosporidium spp were identified as important pathogens commonly seen in Erzurum. Adequate hygienic measures should be taken to reduce the frequency of enteric pathogens. Immunological and molecular tests should be done for a better interpretation of the results. [ABSTRACT FROM AUTHOR] more...
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- 2021
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25. Removal of Giardia spp. cysts and Cryptosporididum spp. oocysts from anaerobic effluent by dissolved air flotation.
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de Oliveira, Gabriela Laila and Daniel, Luiz Antonio
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DISSOLVED air flotation (Water purification) ,OOCYSTS ,GIARDIA lamblia ,GIARDIA ,CRYPTOSPORIDIUM ,UPFLOW anaerobic sludge blanket reactors - Abstract
Lab-scale studies were carried out to investigate the efficiency of dissolved air flotation (DAF) for the removal of Giardia spp. cysts and Cryptosporidium spp. oocysts from anaerobic effluent from the pilot UASB reactor. Raw wastewater, UASB and DAF effluent samples were collected weekly and protozoan (oo)cysts were concentrated using IMS followed by protozoa detection using immunofluorescense assay (IFA). The number of Cryptosporidium spp. oocysts in the raw wastewater was always lower than that of Giardia spp. cysts with 28–33 oocysts L
−1 and 3177–4267 cysts L−1 , respectively. Log10 removal of Giardia cysts utilising polyaluminium chloride (PACl) was higher than that with FeCl3 , but no statistically significant difference between the two coagulants was observed. Cryptosporidium was absent in most of the treated effluent samples. The results indicate that DAF reached more than 2 log of cyst removal. In addition, the results demonstrated that these parasites are prevalent in the study area and E. coli and total coliforms were not good indicator microorganisms in terms of cyst and oocysts numbers. [ABSTRACT FROM AUTHOR] more...- Published
- 2021
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26. Severe weather events and cryptosporidiosis in Aotearoa New Zealand: A case series of space-time clusters.
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Grout L, Hales S, Baker MG, French N, and Wilson N
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- New Zealand epidemiology, Humans, Retrospective Studies, Adult, Child, Preschool, Male, Middle Aged, Child, Female, Aged, Adolescent, Young Adult, Space-Time Clustering, Infant, Disease Outbreaks, Aged, 80 and over, Seasons, Infant, Newborn, Cryptosporidiosis epidemiology, Weather
- Abstract
Occurrence of cryptosporidiosis has been associated with weather conditions in many settings internationally. We explored statistical clusters of human cryptosporidiosis and their relationship with severe weather events in New Zealand (NZ). Notified cases of cryptosporidiosis from 1997 to 2015 were obtained from the national surveillance system. Retrospective space-time permutation was used to identify statistical clusters. Cluster data were compared to severe weather events in a national database. SaTScan analysis detected 38 statistically significant cryptosporidiosis clusters. Around a third (34.2%, 13/38) of these clusters showed temporal and spatial alignment with severe weather events. Of these, nearly half (46.2%, 6/13) occurred in the spring. Only five (38%, 5/13) of these clusters corresponded to a previously reported cryptosporidiosis outbreak. This study provides additional evidence that severe weather events may contribute to the development of some cryptosporidiosis clusters. Further research on this association is needed as rainfall intensity is projected to rise in NZ due to climate change. The findings also provide further arguments for upgrading the quality of drinking water sources to minimize contamination with pathogens from runoff from livestock agriculture. more...
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- 2024
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27. Cryptosporidium oocyst shedding in buffalo calves in Haryana: A case study
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Swain, Krutanjali, Routray, Abhilash, Sahoo, Saraswat, and Ganguly, Subha
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- 2019
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28. Cryptosporidium parvum – zoonotic subtype IIdA15G1 in a Slovakian patient.
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Mravcová, Kristína, Štrkolcová, Gabriela, Mucha, Rastislav, Barbušinová, Eva, Goldová, Mária, Kačírová, Jana, and Maďar, Marián
- Abstract
Introduction and objectives. The parasite Cryptosporidium spp. is an intracellular protozoa which has a broad range of hosts and zoonotic potential. It presents a serious health risk for agricultural workers and veterinarians. The aim of the study was to identify the species and subtypes of Cryptosporidium occurring in a veterinary student who came into contact with calves on a farm. Materials and method. The Ziehl-Neelsen staining technique was employed to confirm the presence of Cryptosporidium oocysts. ELISA test was applied to detect coproantigen in faecal specimens. Nested PCR was used to amplify a small ribosomal subunit (SSU rRNA) and sequencing of the GP60 gene served to identify the zoonotic subtypes. Results. The nested PCR allowed to confirm the C. parvum species; subsequently, the IIdA15G1 zoonotic subtype was identified. Conclusion. This is the first confirmed case in Slovakia of human cryptosporidiosis caused by the unique subtype IIdA15G1. [ABSTRACT FROM AUTHOR] more...
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- 2020
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29. Prevalence of Cryptosporidium spp. in Diarrheic Dogs in Van Province.
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AYAN, Adnan and ORUNÇ KILINÇ, Özlem
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CRYPTOSPORIDIOSIS ,DISEASE prevalence ,ZOONOSES ,DOG diseases ,DIARRHEA in animals ,POLYMERASE chain reaction - Abstract
Copyright of Türkiye Klinikleri Journal of Veterinary Sciences is the property of Turkiye Klinikleri and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) more...
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- 2020
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30. Potential sources of infection with selected zoonotic agents in the veterinary work environment – pilot studies.
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Wójcik-Fatla, Angelina, Sroka, Jacek, Zając, Violetta, Sawczyn-Domańska, Anna, Kloc, Anna, Zwoliński, Jacek, Kłapeć, Teresa, Studzińska, Maria Bernadeta, Chmura, Robert, and Dutkiewicz, Jacek
- Abstract
Abstract Introduction and Objective. The problem of occupational biohazards is very important, especially in the field of agriculture and in human and veterinary medicine. The aim of the study was to determine the potential sources of infection in veterinary professionals with selected zoonotic agents, including: Toxoplasma gondii, Giardia duodenalis, Leptospira spp., Cryptosporidium spp. and Coxiella burnetii. Materials and Method. A total of 50 air samples from barns, piggeries and veterinary surgeries were examined for the presence of Leptospira spp. and C. burnetii DNA. Serum samples of 86 pigs and 80 cows were tested for the presence of antibodies to Leptospira spp. and to phase I and II C. burnetii antigens. Serum of 70 cats were tested for the presence of antibodies to T. gondii and 65 samples of cat faeces for the presence of T. gondii oocysts. The presence of G. duodenalis and Cryptosporidium spp. were examined in 50 of dog faeces and 50 of bovine faeces samples. Results. DNA of Leptospira spp. was detected in 2 air samples from the piggeries (4%). C. burnetii DNA was not found in any sample. Anti-Leptospira spp. antibodies were detected in 51 (59.3%) of examined pigs. Neither anti-Leptospira spp. nor anti-C. burnetii antibodies were found among samples of bovine serum. Anti-T. gondii antibodies was found in 52 cat serum samples (74.3%). Among samples of cat faeces, no T. gondii oocysts were detected. In one sample of cattle stool (2%), G. duodenalis was detected and in another (2%) – Cryptosporidium spp. G. duodenalis was detected in 7 samples (14%) and Cryptosporidium spp. in 2 samples (2%) of dog faeces. Conclusions. The results of this study demonstrate the potential risk of infection with Leptospira spp. in veterinarians working with pigs. Veterinarians could be also be at risk of infection with T. gondii and G. duodenalis. [ABSTRACT FROM AUTHOR] more...
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- 2020
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31. Prevalencia de Cryptosporidium spp., Cystoisospora belli y Cyclospora cayetanensis en pacientes infectados con VIH en el municipio de Veracruz, México
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Zaldívar López, Alejandra, Hernández Romano, Pablo A., Infanzón Ruiz, Raúl, Bravo Sarmiento, Elidé, Torres Hernández, Rosa M., López Banderas, Nayali A., Zaldívar López, Alejandra, Hernández Romano, Pablo A., Infanzón Ruiz, Raúl, Bravo Sarmiento, Elidé, Torres Hernández, Rosa M., and López Banderas, Nayali A. more...
- Abstract
Introduction. The human immunodeficiency virus (HIV) infects and gradually destroy CD4+ cells of the immune system and eventually leading to immunodeficiency. Gastrointestinal opportunistic parasitosis are a frequent cause of morbidity and mortality in HIV+ patients. Objective. To determine the prevalence of the opportunistic parasites Cryptosporidium spp., Cystoisospora belli and Cyclospora cayetanensis, in patients infected with HIV in the municipality of Veracruz. Mexico. Material and methods. 230 non-serial stool samples from patients with HIV were collected. The smears were analysed by direct method and modified Ziehl-Neelsen staining. Results. A prevalence of parasitism of 92.17% was found, 85.22% of Cryptosporidium spp., and C. cayetanensis were identified as mono-parasitism or in association with each other and with other non- opportunistic parasites. Cryptosporidium spp. was the most prevalent parasite (72.60%), followed by C. cayetanensis (57.78%), Endolimax nana (36.52%), Blastocystis spp. (17.83%) and Entamoeba Complex (7 .83%). No samples with Cystoisospora belli were identified. Conclusions. Compared to the only report of opportunistic parasites in the HIV+ population of the Veracruz municipality, there was an increase in the prevalence of Cryptosporidium spp. and a abscense of C. belli. Even with a greater access to antiretroviral treatment, in Mexico, parasitosis continue to have high prevalences. Therefore, routine monitoring of opportunistic parasitism is necessary, using appropriate techniques to avoid underdiagnosis and the risk of comorbidity and mortality that they represent in the HIV+ population., Introducción: El virus de la inmunodeficiencia humana (VIH) infecta y destruye células CD4+ del sistema inmunitario conduciendo gradualmente a una inmunodeficiencia. Las parasitosis oportunistas gastrointestinales son causa frecuente de morbilidad y mortalidad en pacientes VIH+. Objetivo: Determinar la prevalencia de los parásitos oportunistas Cyclospora cayetanensis, Cryptosporidium spp. y Cystoisospora belli en pacientes con VIH en el municipio de Veracruz. México. Material y métodos: Se recolectaron 230 muestras no seriadas de heces de pacientes con VIH. Los extendidos fueron análizados por método directo y tinción de Ziehl-Neelsen modificada. Resultados: Se encontró una prevalencia de parasitosis del 92.17%, en el 85.22% se identificó a Cryptosporidium spp. y Cyclospora cayetanensis, como mono-parasitismo o en asociación entre ellos, y con otros parásitos no oportunistas. Cryptosporidium spp. fue el parásito de mayor prevalencia (72.60%), seguido de C. cayetanensis (57.78%), Endolimax nana (36.52%), Blastocystis spp. (17.83%) y el Complejo Entamoeba (7.83%). No se identificaron muestras con Cystoisospora belli. Conclusiones: Hubo un aumento en la prevalencia de Cryptosporidium spp. y una disminución en el Cystoisospora belli en la población VIH+ del municipio de Veracruz. Aún con el mayor acceso a tratamiento antiretroviral en Mexico, las parasitosis tiene una alta prevalencia y se require del monitoreo de parásitos oportunistas mediante técnicas adecuadas para evitar el subdiagnóstico y el riesgo de comorbilidad y mortalidad que representan en la población VIH+ more...
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- 2023
32. Comparative evaluation of conventional staining method and enzyme linked immunosorbent assay kits for the detection of bovine cryptosporidiosis
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Thakre, B. J., Solanki, J.B., Kumar, N., and Vargese, A.
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- 2017
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33. Prevalencia de Cryptosporidium spp. y Giardia spp. en terneros, y su presencia en agua y en niños con problemas digestivos en el cantón San Fernando, Ecuador
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Teófilo E. Palacios O.
- Subjects
Cryptosporidium spp ,Giardia spp. ,prevalencia ,terneros ,ooquistes ,quisten en agua ,problemas digestivos en niños ,cantón San Fernando ,Science (General) ,Q1-390 ,Social sciences (General) ,H1-99 - Abstract
Objetivo: Determinar la prevalencia de Cryptosporidium spp. y Giardia spp. en heces de terneros de 0-4 meses de edad, como factor contaminante de los recursos hídricos de uso de la población humana de 2-6 años de edad, en el cantón San Fernando, provincia del Azuay. Metodología: Es un estudio epidemiológico de corte transversal-descriptivo, realizado durante el período septiembre del 2013 a abril del 2014. Se utilizó una muestra de 120 terneros de 0 a 4 meses de edad, que de acuerdo a los datos de AGROCALIDAD es la totalidad de terneros del cantón; 42 niños de 2 a 6 años de edad con problemas digestivos en su visita al centro de salud y 40 litros de agua obtenida de la zona de captación en el sector del Hato de la Virgen. En terneros y niños se utilizó materia fecal que fue tratada para realizar la coloración de Ziehl-Neelsen para Cryptosporidium spp. y la técnica de Ritchie para Giardia spp. En el agua se determinó la cantidad de quistes y ooquistes /100 ml por el método EPA 1623. Los niños fueron discriminados para el estudio según el origen (rural o urbano). Se aplicó una estadística descriptiva con la ayuda del software SPSS versión 22. Resultados: Se determinó que en terneros existe una prevalencia del 93.3% de Cryptosporidium spp., (112 casos positivos) y de 76.7% de Giardia spp. (92 casos positivos). En agua, se determinó la presencia de 5 ooquistes de Cryptosporidium spp./100 ml y 10 quistes de Giardia spp./100 ml. La prevalencia de Cryptosporidium spp. en los niños alcanzó una media del 14.3%, mientras que la de Giardia spp. fue del 33.3%. Conclusiones: El estudio demostró una alta prevalencia de Cryptosporidium spp. y Giardia spp. en los terneros, quienes serían en buena medida los responsables de la alta concentración en agua y de la alta prevalencia en niños con problemas digestivos en el cantón San Fernando. more...
- Published
- 2017
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34. Assessment of Giardia and Cryptosporidium Assemblages/ Species and Their Viability in Potable Tap Water in Beni-Suef, Egypt Using Nested PCR/RFLP and Staining
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Doaa HAMDY, Ayman El-BADRY, and Wegdan ABD EL WAHAB
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Cryptosporidium spp ,Tap water ,Egypt ,Giardia ,Infectious and parasitic diseases ,RC109-216 - Abstract
Background: The protozoan Giardia and Cryptosporidium are responsible for most water-borne diseases all over the world. The extent and number of outbreaks of waterborne diseases suggests a significant risk of their potential transmission via drinking water. This study aimed to document the prevalence and viability of Giardia and Cryptosporidium (oo) cysts in tap water samples in Beni-Suef Governorate, Egypt and to detect the predominant Giardia and Cryptosporidium assemblages/species using nested PCR/ Restriction Fragment Length Polymorphism (RFLP) confirmed by further sequencing of positive samples. Methods: A total of 80 tap water samples were collected throughout a year from four big centers and filtered using the membrane filtration method. Samples were stained by Lugol’s iodine, Modified Zeihl-Neelsen (MZN) (to detect prevalence) and trypan blue stain (to detect viability). Nested PCR-RFLP and sequencing were used for molecular characterizations and genotyping of the detected Giardia and Cryptosporidium. Results: Giardia and Cryptosporidium DNA was detected in 20 (25%) and 29 (36.3%) samples respectively, with predominance of Giardia assemblage B (85%) and C. hominis (75.9%). The prevalence and viability of both parasites (oo) cysts showed seasonality which peaked in summer and were greater in Beba center and in rural areas. Conclusion: To our knowledge, no studies have been done in these areas before. The anthroponotic transmission has an important role in giardiasis and cryptosporidiosis epidemiology in this studied area. more...
- Published
- 2019
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35. Increased number of cryptosporidiosis cases with travel history to Croatia might be related to swimming pools, Germany, 2023.
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Schoeps A, Röbl K, Walter N, Neute A, Walter B, Freudenau I, Jurke A, Klier C, Heinmüller P, Saeed S, Metz J, Wilking H, and Zanger P
- Subjects
- Humans, Croatia epidemiology, Disease Outbreaks, Case-Control Studies, Germany epidemiology, Cryptosporidiosis diagnosis, Cryptosporidiosis epidemiology, Swimming Pools, Cryptosporidium genetics
- Abstract
In August and September 2023, an unusually high number of cryptosporidiosis cases identified by routine German surveillance had travelled to Croatia (n = 23). Nine cases had stayed in the same camping resort and seven further cases had stayed at other camping sites within 15 km. Based on our standardised questionnaires, the most likely source of infection was swimming pools (93%). Further environmental investigations on site might reveal potential common sources of contamination that could be targeted by control measures. more...
- Published
- 2024
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36. Prevalence and Molecular Characterization of Cryptosporidium spp., Giardia duodenalis, and Enterocytozoon bieneusi in Diarrheic and Non-Diarrheic Calves from Ningxia, Northwestern China
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Guo, Haihui Gao, Gaoxing Liang, Na Su, Qirui Li, Dong Wang, Jiandong Wang, Long Zhao, Xiaodong Kang, and Kangkang
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Cryptosporidium spp ,G. duodenalis ,E. bieneusi ,prevalence ,calf ,diarrheic - Abstract
Cryptosporidium spp., Giardia duodenalis, and Enterocytozoon bieneusi are significant parasitic gastrointestinal pathogens with global distribution in humans and domestic animals, including calves. The main symptoms of calf infection are severe diarrhea, dehydration, growth retardation, and sometimes even death. To date, there has been limited information on the prevalence of Cryptosporidium spp., G. duodenalis, and E. bieneusi infections in calves in Ningxia, China, especially between diarrheic and non-diarrheic calves. A total of 438 fecal samples were collected from diarrheic (201) and non-diarrheic (237) calves in Ningxia. PCR and DNA sequencing were used to find the prevalence of Cryptosporidium spp. at 46.8% (205/438), G. duodenalis at 16.9% (74/438), and E. bieneusi at 10.0% (44/438). The prevalence of Cryptosporidium spp. infection in diarrheic and non-diarrheic calves was 54.0% (128/237) and 38.3% (77/201), respectively, and statistical analysis showed a positive correlation between the prevalence of Cryptosporidium spp. infection and calf diarrhea (p < 0.01). However, in this study, there was no statistical correlation between the prevalence of G. duodenalis infection as well as E. bieneusi infection and calf diarrhea (p > 0.05). Furthermore, four known Cryptosporidium species were successfully identified by comparing them with SSU rRNA gene sequences, including C. parvum, C. bovis, C. ryanae, and C. andersoni. In addition, all 74 G. duodenalis-positive samples were identified as assemblage E by comparative analysis of bg gene sequences. Among the 44 E. bieneusi-positive samples sequenced in the present study, 4 distinct E. bieneusi genotypes were successfully identified by comparative analysis of ITS sequences, including 3 known genotypes (J, BEB4, and N) and 1 novel genotype, the latter of which was identified and designated as NX1. These findings indicated that the high genetic diversity and complex population structures of Cryptosporidium spp., G. duodenalis, and E. bieneusi in Ningxia diarrhea calves and non-diarrhea calves, which provide new data for understanding the epidemiological status of Cryptosporidium spp., G. duodenalis, and E. bieneusi in Ningxia calves. more...
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- 2023
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37. Molecular Identification of Cryptosporidium spp., and Giardia duodenalis in Dromedary Camels (Camelus dromedarius) from the Algerian Sahara
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Sadiya Maxamhud, Nassiba Reghaissia, AbdElkarim Laatamna, Houssem Samari, Nacira Remdani, Eleni Gentekaki, and Anastasios D. Tsaousis
- Subjects
Camelus dromedarius ,Cryptosporidium spp ,Giardia duodenalis ,Entamoeba histolytica ,genotyping ,prevalence ,Algeria ,General Earth and Planetary Sciences ,General Environmental Science - Abstract
(1) Intestinal microbial parasites are major contributors to the global burden of gastrointestinal disease. Such infections are mainly caused by Cryptosporidium, Giardia duodenalis, and Entamoeba histolytica. These parasites are transmitted either directly or indirectly through oral–fecal routes. Previous reports suggested that camels could play a role in the zoonotic transmission of various clinically and veterinary important intestinal parasites, however, limited data are available on intestinal infections of camels, particularly on a molecular level. We aimed to explore the occurrence of these three parasites in camels (Camelus dromedarius) in Algeria. (2) A total of 68 samples—63 stool samples from camels and five from the environment—were collected from two desert regions in Algeria and analyzed using PCR and qPCR methods. (3) Overall, 7% of the camels tested positive for zoonotic subtypes of Cryptosporidium spp., while 16% of the camels tested positive for G. duodenalis. Two environmental samples also tested positive for G. duodenalis. None of the samples were positive for Entamoeba histolytica. (4) Our results provide one of the first molecular-based identification of these gut parasites in dromedary camels in Algeria. The presence of G. duodenalis in the host and the environment unveils, in part, the circulation route of this parasite. Our results will spearhead further investigations into the prevalence and epidemiology of gut parasites in hoofed animals and raise questions concerning their role in health and disease in the area. more...
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- 2023
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38. Investigation of Cryptosporidium spp. positivity in Formol-Fixed Stool samples by Polymerase Chain Reaction Method.
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Karaman, Ülkü, Kolören, Zeynep, and Ayaz, Emine
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- *
POLYMERASE chain reaction , *RHINOVIRUSES , *EIMERIA , *CRYPTOSPORIDIUM , *SCHOOL children , *WATER pollution , *CRYPTOSPORIDIOSIS - Abstract
Objective: It was aimed to determine Cryptosporidium spp., which is thought to cause digestive system complaints in primary school children, by Modified Acid-Fast (MAF) staining method and Polymerase Chain Reaction (PCR). Method: One hundred sixty-four stool samples taken from children were examined under light microscopy with MAF. Positive samples were fixed in 10% formalin and stored at + 4 °C until analysis. These samples were then purified using the Sucrose Gradient method and, DNA isolation was performed using the modified QIAamp DNA Mini Kit (Qiagen) protocol. In order to increase the sensitivity of the study, the SSU rRNA gene region was amplified by PCR method. Results: Microscopic examination showed that 89 of the 164 stool samples (54,26%) were found to be positive for Cryptosporidium spp.oocysts. Eightynine samples which were found to be positive by microscopic examination and fixed in %10 formalin were studied by PCR, and 74 (45,12%) of them were positive. Conclusions: Cryptosporidiosis, which is known to cause severe infections, especially in immunocompromised individuals and children, is transmitted by the fecal-oral route from infected humans and animals, and contaminated water and nutrients. Cryptosporidiosis, which is the result of fecal pollution, causes a great risk for human health even with a low number of oocysts due to the fact that oocysts are resistant to chlorine and they can survive for a long time in the outer environment. It was observed that primary school children living in the area of the study were under risk for cryptosporidiosis. [ABSTRACT FROM AUTHOR] more...
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- 2019
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39. Prevalence of Cryptosporidium, Giardia, Blastocystis, and trichomonads in domestic cats in East China.
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Wenchao LI, Xinchao LIU, Youfang GU, Junlong LIU, and Jianxun LUO
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CRYPTOSPORIDIUM ,CATS ,BLASTOCYSTIS ,PROTOZOAN diseases ,GIARDIA ,MIXED infections - Abstract
The cat is a reported reservoir for several zoonotic pathogens, including Cryptosporidium spp., Giardia duodenalis, and Blastocystis sp. These parasites represent a significant, but often neglected, threat to humans and animals. Furthermore, Tritrichomonas foetus has been described inhabiting the digestive tract of cats, and may be causative agents of gastrointestinal symptoms. However, scant data are available concerning the molecular epidemiology of these parasites in domestic cats in China. This study examined fecal samples from domestic cats in Eastern China to unravel the molecular epidemiology of four protozoans. Of the 346 samples examined, 47 (13.6%) were positive for the detected pathogens, including 8 (2.3%), 5 (1.4%), 2 (0.6%), and 35 (10.1%) samples positive for Cryptosporidium spp., G. duodenalis, Blastocystis sp., and T. foetus, respectively. Co-infection with Cryptosporidium spp. and T. foetus was detected in three cats, no other mixed infections were observed. No age, sex or fecal condition predisposition was observed with any of the four pathogens. The species/assemblages/subtypes/genotypes were C. felis, Assemblage A and F, ST1, and cat genotype for Cryptosporidium spp., G. duodenalis, Blastocystis sp., and T. foetus detected in this study, respectively. The presence of zoonotic species/assemblages/subtypes/genotypes poses a threat to public health. These findings provide useful information for the design of prevention and control strategies to reduce the burden of protozoal infections in cats. [ABSTRACT FROM AUTHOR] more...
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- 2019
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40. First Molecular Characterization of Cryptosporidium spp. in Patients Living with HIV in Honduras
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Sergio Betancourth, Osman Archaga, Wendy Moncada, Vilma Rodríguez, and Gustavo Fontecha
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Cryptosporidium spp ,C. parvum ,gp60 ,18S ribosomal ,cowp ,HIV/AIDS ,Medicine - Abstract
Cryptosporidiosis is one of the most important causes of gastroenteritis in the world, especially in low- and middle-income countries. It is caused by the Apicomplexan parasite Cryptosporidium spp., and mainly affects children and immunocompromised people, in whom it can pose a serious threat to their health, or even be life threatening. In Honduras, there are no data on parasite species or on molecular diversity or Cryptosporidium subtypes. Therefore, a cross-sectional study was conducted between September 2019 and March 2020 for the molecular identification of Cryptosporidium spp. in 102 patients living with HIV who attended a national hospital in Tegucigalpa. Stool samples were analyzed by direct microscopy, acid-fast stained smears, and a rapid lateral flow immunochromatographic test. All samples that tested positive were molecularly analyzed to identify the species and subtype of the parasite using three different markers: gp60, cowp, and 18Sr. PCR products were also sequenced. Four out of 102 samples (3.92%) were positive for Cryptosporidiumparvum, and all were assigned to subtype IIa. These findings suggest a possible zoonotic transmission in this population. more...
- Published
- 2021
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41. 7G7 - Estimación de la incidencia de enfermedad diarreica aguda en Colombia relacionada con riesgos microbiológicos en el agua para consumo, empleando modelos de estimación probabilística, 2022.
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Gamboa-Marín, Andrea, Rufina Rodríguez, Flor, and Camilo Rodríguez, Cristian
- Abstract
Copyright of Biomédica: Revista del Instituto Nacional de Salud is the property of Instituto Nacional de Salud of Colombia and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) more...
- Published
- 2023
42. Detection and identification of six Cryptospordium species in livestock in Slovakia by amplification of SSU and GP60 genes with the use of PCR analysis
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Oľga Danišová, Alexandra Valenčáková, and Antónia Petrincová
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Cryptosporidium spp ,cattle ,pig ,zoonotic potential ,gene SSU ,gene GP60 ,Agriculture ,Environmental sciences ,GE1-350 - Abstract
Introduction In this study we examined 200 faecal samples from pigs and calves with suspected cryptosporidiosis were examined by the PCR methods: nested PCR for amplification of SSU region; nested PCR for amplification of GP60 region; and with restriction analysis of DNA (PCR-RFLP). The sequencing identified the following species: Cryptosporidium muris (2), Cryptosporidium andersoni (1), Cryptosporidium bovis (4), Cryptosporidium suis (2), Cryptosporidium scrofarum (10), mixed infection caused by C. scrofarum and C. muris (1),and Cryptosporidium parvum (10) genotype A subtype IIaA17G2R1. Results and conclusions The findings suggest that livestock can be an important source of zoonotic species or genotypes of Cryptosporidium , which may adversely affect the public health of human populations. This is the first time in our country that the Cryptosporidium species has been identified in livestock in Slovakia. The identification and genotyping of this pathogen in Slovakia, completes the epidemiological situation in Europe for Cryptosporidum species. more...
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- 2016
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43. Survey of Zoonotic Diarrheagenic Protist and Hepatitis E Virus in Wild Boar (Sus scrofa) of Portugal
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Sérgio Santos-Silva, Danny Franciele da Silva Dias Moraes, Pedro López-López, Josman D. Palmeira, Rita T. Torres, Maria São José Nascimento, Alejandro Dashti, David Carmena, Antonio Rivero-Juarez, João R. Mesquita, and Fundação para a Ciência e Tecnologia (Portugal) more...
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Emerging infectious diseases ,Balantioides coli ,Cryptosporidium spp ,Blastocystis sp ,General Veterinary ,Zoonotic ,hepatitis E virus ,zoonotic ,emerging infectious diseases ,Hepatitis E virus ,Animal Science and Zoology - Abstract
Enteropathogenic parasites and viruses have been frequently reported in swine and can infect a wide range of mammals, including humans. Among the wide variety of parasites infecting swine, diarrhoeagenic protists are among those that cause significant morbidity. Hepatitis E virus (HEV) has also been reported both in domestic pigs and wild boar and is known to have an important public health significance. These agents share the fecal−oral transmission route, but data on their fecal shedding and circulation pathways are still lacking or incomplete. Hence, the aim of the present study was to characterize the presence of microeukaryotes and HEV in the wild boar of Portugal. Wild boar stool samples (n = 144) were obtained during the official hunting seasons (October to February) in 2018/2019, 2019/2020, and 2021/2022 and tested for Cryptosporidium spp., Balantioides coli, Giardia duodenalis, Blastocystis sp., Enterocytozoon bieneusi and HEV by molecular assays, followed by sequencing and phylogenetic analysis. We have detected Cryptosporidium scrofarum (1.4%, 95% CI: 0.2−4.9), B. coli (14.6%, 95% CI: 9.2−21.4), Blastocystis ST5 (29.2%, 95% CI: 21.9−37.2) and HEV genotype 3 (2.8%, 95% CI: 0.7−6.9; subgenotypes 3e and 3m). Co-infections were observed in thirteen animals where two were positive for both HEV and B. coli, one was positive for both C. scrofarum and Blastocystis ST5, and ten were positive for both B. coli and Blastocystis ST5. Giardia duodenalis and E. bieneusi were not detected in the surveyed wild boar population. As far as we know, this is the first report describing protist infections by Cryptosporidium spp., B. coli, and Blastocystis sp., as well as the first identification of the emerging HEV genotype 3m in wild boar of Portugal. The present work shows that potentially zoonotic protozoa and HEV are circulating in wild boar populations in Portugal. Awareness and epidemic-surveillance network implementation measures targeting wild boar are needed to prevent the spread of these pathogenic agents to humans. This research was funded by Fundação para Ciência e Tecnologia (FCT), grant number 2021.09461.BD. Sí more...
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- 2023
44. Cryptosporidium spp
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De Felice, Lorena Alejandra, Basset, Carina, and Unzaga, Juan Manuel
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Ciencias Veterinarias ,Biología ,Protozoario ,Cryptosporidium spp - Abstract
Cryptosporidium spp., protozoario que pertenece al phylum Aplicomplexa, parasita a hospedadores vertebrados, incluido el hombre. Los individuos infectados por Cryptosporidium spp. pueden presentar un amplio espectro de manifestaciones clínicas dependiendo de la especie del parásito y también del tipo, edad y respuesta inmune del hospedador (Xiao et al., 2004)., Facultad de Ciencias Veterinarias, Facultad de Ciencias Naturales y Museo more...
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- 2023
45. Synchronous Identification of Entamoeba histolytica, Giardia intestinalis, and Cryptosporidium spp. in Stool Samples Using a Multiplex PCR Assay
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Amir BAIRAMI, Sasan REZAEI, and Mostafa REZAEIAN
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Diagnostics ,Entamoeba histolytica ,Giardia intestinalis ,Cryptosporidium spp ,Multiplex PCR ,Infectious and parasitic diseases ,RC109-216 - Abstract
Background: Diarrheal disease annually causes 760000 deaths in children, and 1700 million new cases are reported each year worldwide. Among the parasites, Entamoeba histolytica, Giardia intestinalis, and Cryptosporidium spp. are the most important infectious agents leading to diarrhea. Clinical presentations due to these parasites are more or less similar, and microscopy is not as much as sensitive for the detection. The aim of this study was to set up and evaluate a Multiplex PCR Assay for Synchronous Identification of Entamoeba histolytica, Giardia intestinalis, and Cryptosporidium spp. in Stool Samples Methods: Samples were obtained from different sources such as culture media and patient stool samples. Primer pairs were designed using primer-BLAST, and for the extraction of DNA, the QIAamp DNA stool mini kit was used. The study was conducted in Tehran, Iran and completed in 2016. Results: The current multiplex PCR assay for the detection of E. histolytica achieved sensitivity and specificity of 86.36% (95% CI: 65.09% to 97.09) and 95.74 % (95% CI: 85.46% to 99.48%), respectively. Sensitivity and specificity of the test for G. intestinalis was 90.91% (95% CI: 70.84% to 98.88%) and 95.74% (95%CI: 85.46% to 99.48%), respectively, and for the detection of Cryptosporidium, multiplex PCR showed a sensitivity of 90.91% (95% CI: 70.84% to 98.88%) and specificity of 95.74% (95%CI: 85.46% to 99.48%). Conclusion: Multiplex PCR in this study showed admissible sensitivity and specificity for the detection of E. histolytica, G. intestinalis, and Cryptosporidium spp. in fecal samples. more...
- Published
- 2018
46. Investigation of Zoonotic Cryptosporidium and Giardia intestinalis Species and Genotypes in Cats (Felis catus)
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Delibaşı Kökçü, Nesrin, Yıldırım, Alparslan, İnci, Abdullah, Düzlü, Önder, Çiloğlu, Arif, Pekmezci, Gökmen Zafer, Önder, Zuhal, Yetişmiş, Gamze, and Pekmezci, Didem
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molecular characterization ,cryptosporidium spp ,genotyping ,giardia intestinalis ,parasitic diseases ,cat ,Medicine ,Infectious and parasitic diseases ,RC109-216 - Abstract
Objective:Giardia intestinalis and Cryptosporidium spp. are important zoonotic protozoan parasites that infect humans and various animals. We investigated the occurrence of G. intestinalis and Cryptosporidium spp. infection in cats. To provide data on the zoonotic transmission dynamics of these parasites, genotypes of the detected isolates were investigated through DNA sequence characterization.Methods:A total of 100 fecal samples were collected from cats between June and October 2020 in Kayseri and Samsun provinces. Fecal samples were examined by nested polymerase chain reaction (PCR), targeting the β-giardin gene of G. intestinalis and small subunit (SSU) rRNA gene of Cryptosporidium spp. All PCR products were sequenced for genotyping.Results:Of the samples examined, Giardia intestinalis was determined in 8 samples (8.0%), whereas none of the samples were found positive for Cryptosporidium spp. Sequence analyses of the β-giardin PCR products indicated that all G. intestinalis isolates were classed into the zoonotic assemblage B.Conclusion:This study adds to the current data on the molecular epidemiology of cryptosporidiosis and giardiasis in cats. The findings also highlight the potential risk of cats for public health concerning the zoonotic transmission dynamics of G. intestinalis. more...
- Published
- 2021
47. Occurrence of Cryptosporidium spp., Giardia spp. and other pathogenic intestinal parasites in the Beberibe River in the State of Pernambuco, Brazil
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Dayana Andrade de Freitas, Anderson Luiz Ribeiro de Paiva, José Adson Andrade de Carvalho Filho, Jaime Joaquim da Silva Pereira Cabral, and Francisca Janaína Soares Rocha
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Protozoa ,Beberibe River ,Cryptosporidium spp ,Arctic medicine. Tropical medicine ,RC955-962 - Abstract
INTRODUCTION: Transmission of pathogenic protozoa and helminths by water is a serious public health problem. In this study, we analyzed the presence of these organisms in the Beberibe River in Pernambuco, Brazil. METHODS: Parasite analysis was performed using the Hoffman, Pons, & Janer method followed by centrifugation and preparation of slides by staining with acetic acid and Lugol's solution. Protozoan oocysts were isolated by the modified Ziehl Neelsen method. RESULTS: Cryptosporidium spp., Giardia spp. and other parasites were found in the Beberibe River. CONCLUSIONS: Sanitation companies must assess pathogenic intestinal parasites in water basins providing public water and subsequently develop improved treatment systems for removal of such parasites. more...
- Published
- 2015
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48. Deteção de enteroparasitas e caracterização genética de Cryptosporidium spp. e Giardia duodenalis em crianças até aos 14 anos, com diarreia, em Moçambique
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NHAMBIRRE, Ofélia Luís, DEUS, Nilsa Olívia Razão de, MATOS, Olga Maria Guerreiro de, and COSTA, Maria Luísa Lobo Ferreira da
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Cryptosporidium spp ,Moçambique ,Crianças ,Parasitologia ,Ciências Médicas [Domínio/Área Científica] ,Diarreias ,Giardia duodenalis - Abstract
As infeções parasitárias causadas por helmintas intestinais, estão entre as mais prevalentes infeções lentas em humanos no mundo, principalmente nos países em desenvolvimento, incluindo Moçambique e, com as infeções causadas por protozoários intestinais, contribuem significativamente para grande carga de doenças gastrointestinais nestes países, sobretudo nas crianças. Giardia duodenalis e Cryptosporidium spp. são dois organismos protozoários que infetam os humanos e diversas espécies animais e são conhecidos por apresentar um elevado potencial em causar diarreia. Existem em Moçambique alguns estudos sobre a epidemiologia das infeções provocadas por parasitas intestinais, contudo, esta informação é muito fragmentada e escassa sobretudo no que diz respeito à epidemiologia molecular de G. duodenalis e Cryptosporidium spp. Este estudo teve como objetivo geral, determinar a frequência e os fatores relacionados com a infeção por parasitas intestinais (IPI) e caracterizar geneticamente G. duodenalis e Cryptosporidium spp. circulantes em crianças até aos 14 anos de idade com doença diarreica, nas regiões sul, centro e norte de Moçambique. Para o estudo foram analisadas 1424 amostras de fezes diarreicas (do projeto ViNaDia, Moçambique) recrutadas em crianças até 14 anos em seis hospitais públicos das regiões sul, centro e norte de Moçambique. Uma única amostra de fezes foi colhida por criança e examinada por Microscopia Óptica (M.O.) usando a concentração de formol-éter e a técnica de coloração de Ziehl-Neelsen modificada para identificar parasitas intestinais, nomeadamente coccídeos e Cryptosporidium. As características sociodemográficas foram obtidas por meio de questionários. Para caracterização molecular foram utilisadas amostras positivas por M.O. assim como por imunoensaio enzimático (ELISA) (de outro subestudo do projeto ViNaDia). A caracterização genética foi realizada por nested-PCR com alvos nos genes β-giardina de G. duodenlais e ssu rRNA e gp60 de Cryptosporidium spp. Análises uni e bivariadas e regressão logística binária foram realizadas para descrever a população e determinar os fatores de risco. Níveis de significância de 5% foram considerados significativos. Pelo menos uma IPI foi detetada em 19,2% (273/1424) das crianças. Cryptosporidium spp. foi o parasita mais comum (8,1%; 115/1424). Poliparasitismo foi observado em 26,0% (71/273) das crianças estudadas, sendo a coinfeção Ascaris lumbricoides e Trichuris trichiura (26,8%; 19/71) a mais comum. A idade e província foram relacionadas com a presença de IPI (p-valor < 0,05). A maior ocorrência de IPI foi observada na estação das chuvas (outubro a março) com 23,1% (191/828) em relação aos períodos secos (abril a setembro) com 13,6% (80/588) (p-valor < 0,001 ). O subtipo IaA9G3 de C. hominis foi o dominante nas crianças infetadas por Cryptosporidium e o subgenótipo AII de G. duodenalis foi o mais observado (56,6%; 13/23) na população pediátrica moçambicana. Cryptosporidium spp. e a combinação A. lumbricoides/T. trichiura foram os principais parasitas intestinais observados em crianças hospitalizadas com diarreia em Moçambique. Os dados de genotipagem sugerem que as principais vias de transmissão de G. duodenalis e Cryptosporidium em Moçambique são antroponóticas. Parasitic infections caused by intestinal helminths are among the most prevalent sluggish infections in humans in the world, mainly in developing countries, including Mozambique, and with infections caused by intestinal protozoa, they contribute significantly to large burdens of gastrointestinal diseases in these countries, especially in children. Giardia duodenalis and Cryptosporidium spp. are two protozoan organisms that infect humans and several animal species and are known to have a high potential to cause diarrhea. There are some studies in Mozambique on the epidemiology of diseases caused by intestinal parasites; however, this information is very fragmented and scarce regarding the molecular epidemiology of G. duodenalis and Cryptosporidium spp. This study aimed to determine the frequency and factors related to intestinal parasites infection and to characterize genetically G. duodenalis and Cryptosporidium spp. circulating in children up to 14 years of age with diarrheal disease in southern, central and northern Mozambique. For this purpose, 1424 diarrheal stool samples (included in the ViNaDia project, Mozambique) were collected from children up to 14 years old in six public hospitals in the southern, central and northern regions of Mozambique. A single stool sample was collected per child and examined by light microscopy using formalin-ether concentration and modified Ziehl-Neelsen techniques to identify intestinal parasites, namely coccidia and Cryptosporidium. Sociodemographic characteristics were obtained through questionnaires. For molecular characterization positive samples from light microscopy as well as by ELISA (from another ViNaDia sub study) were used. Genetic characterization was performed by nested-PCR targeting β-giardin gene for G. duodenlais and SSUrRNA and gp60 genes for Cryptosporidium spp. Univariate and bivariate analysis and binary logistic regression were performed to describe the population and determine risk factors. Significance levels of 5% were considered significant. A single IPI was detected in 19.2% (273/1424) of children. Cryptosporidium spp. was the most common parasite (8.1%; 115/1424). Polyparasitism was observed in 26.0% (71/273) of the children, with Ascaris lumbricoides and Trichuris trichiura (26.8%; 19/71) being the most common co-infection. Age and province were related to IPI (p-value < 0.05). The highest occurrence of IPI was observed in the wet season (October to March) with 23.1% (191/828) compared to the dry season (April to September) with 13.6% (80/588) (p-value < 0.001). The IbA9G3 subtype of C. hominis was the most dominant in children infected with Cryptosporidium and sub assemblage AII 56.6% (13/23) more frequently observed in children infected with G. duodenalis in the Mozambican pediatric population. Cryptosporidium spp. and the combination A. lumbricoides/T. trichiura were the main intestinal parasites observed in children hospitalized with diarrhea in Mozambique. Genotyping data suggest that the main routes of transmission of G. duodenalis and Cryptosporidium in Mozambique are anthroponotic. more...
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- 2022
49. Enteropathogens associated with neonatal calves diarrhea in Tiaret area (Western Algeria).
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Selles, Sidi Mohammed Ammar, Kouidri, Mokhtaria, Belhamiti, Belkacem Tahar, Amrane, Amar Ait, Benia, Ahmed Redha, Hammoudi, Si Mohamed, Kaidi, Rachid, and Boukraa, Laid
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DIAGNOSIS of diseases in calves ,DISEASE prevalence ,DIARRHEA ,PATHOGENIC microorganisms ,ANTI-infective agents ,CATTLE - Abstract
Copyright of Veterinaria is the property of University of Sarajevo, Veterinary Faculty and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) more...
- Published
- 2018
50. Prevalence and associated risk factors of cryptosporidiosis among cattle in the region of eastern Algeria.
- Author
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Hocine, Abderrazek, Bouzid, Riad, Aggad, Hebib, Khelef, Djamel, and Talhi, Hamida
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CRYPTOSPORIDIOSIS ,DISEASE prevalence ,ANIMAL breeding ,INFECTIOUS disease transmission ,DIAGNOSIS of diseases in calves ,DISEASE risk factors - Abstract
Copyright of Veterinaria is the property of University of Sarajevo, Veterinary Faculty and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) more...
- Published
- 2018
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