99 results on '"Cuiling Yu"'
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2. An Improved Squeaky Wheel Optimization Approach to the Airport Gates Assignment Problem.
- Author
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Cuiling Yu and Xueyan Song
- Published
- 2013
- Full Text
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3. Recent progress of composite solid polymer electrolytes for all-solid-state lithium metal batteries
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Zhang Chuanjian, Qingjiang Yu, Yao Yi, Long Huijin, Jiang Bin, Jiang Kecheng, Cuiling Yu, and Chen Xianjin
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Materials science ,Composite number ,chemistry.chemical_element ,02 engineering and technology ,General Chemistry ,Electrolyte ,010402 general chemistry ,021001 nanoscience & nanotechnology ,Electrochemistry ,01 natural sciences ,Energy storage ,0104 chemical sciences ,Chemical engineering ,chemistry ,Thermal ,Ionic conductivity ,Lithium ,0210 nano-technology ,Electrochemical window - Abstract
In comparison with lithium-ion batteries (LIBs) with liquid electrolytes, all-solid-state lithium batteries (ASSLBs) have been considered as promising systems for future energy storage due to their safety and high energy density. As the pivotal component used in ASSLBs, composite solid polymer electrolytes (CSPEs), derived from the incorporation of inorganic fillers into solid polymer electrolytes (SPEs), exhibit higher ionic conductivity, better mechanical strength, and superior thermal/electrochemical stability compared to the single-component SPEs, which can significantly promote the electrochemical performance of ASSLBs. Herein, the recent advances of CSPEs applied in ASSLBs are presented. The effects of the category, morphology and concentration of inorganic fillers on the ionic conductivity, mechanical strength, electrochemical window, interfacial stability and possible Li+ transfer mechanism of CSPEs will be systematically discussed. Finally, the challenges and perspectives are proposed for the future development of high-performance CSPEs and ASSLBs.
- Published
- 2021
4. A Brief Analysis of the Naming of ‘New Generation’ Literature
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CuiLing Yu
- Subjects
General Medicine - Published
- 2021
5. A Research on Synergic and Spiral-Propulsion Mechanism of Enterprise Information Systems Growth.
- Author
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Xinhua Bi and Cuiling Yu
- Published
- 2007
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6. Active Rehabilitation of Upper Limbs After Stroke Based on Brain Computer Interface
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Jiaxian Chen, Yuxuan Cai, Shuang Bai, Cuiling Yu, and Jialong Huang
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medicine.medical_specialty ,Neuropsychology and Physiological Psychology ,Physical medicine and rehabilitation ,Rehabilitation ,business.industry ,Physiology (medical) ,General Neuroscience ,medicine.medical_treatment ,medicine ,medicine.disease ,business ,Stroke ,Brain–computer interface - Published
- 2021
7. Finite element analysis of resistivity measurement with four point probe in a diamond anvil cell
- Author
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Xiaowei Huang, Chunxiao Gao, Ming Li, Chunyuan He, Aimin Hao, Dongmei Zhang, Cuiling Yu, Yue Wang, Chong Sang, Xiaoyan Cui, and Guangtian Zou
- Subjects
Finite element method -- Usage ,Electric resistance -- Measurement ,Semiconductors -- Electric properties ,Physics - Abstract
The finite element analysis was employed to study the steady current field distribution under the configuration of four point probe method for resistivity measurement in a diamond anvil cell (DAC). It was found that the finite size of the electrode could lead to significant errors in resistivity measurement for semiconducting samples and the probe spacing is a key factor in the resistivity measurement accuracy for samples in DAC.
- Published
- 2007
8. Single-Layer TiO2 Film Composed of Mesoporous Spheres for High-Efficiency and Stable Dye-Sensitized Solar Cells
- Author
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Qingjiang Yu, Haigang Wu, Y.D. Huang, Shiyong Gao, Jinzhong Wang, Peng Wang, Shujie Jiao, Cuiling Yu, Jianan Wang, and Xitian Zhang
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Materials science ,Renewable Energy, Sustainability and the Environment ,General Chemical Engineering ,Nanoparticle ,02 engineering and technology ,General Chemistry ,Electrolyte ,010402 general chemistry ,021001 nanoscience & nanotechnology ,01 natural sciences ,0104 chemical sciences ,law.invention ,chemistry.chemical_compound ,Crystallinity ,Dye-sensitized solar cell ,Chemical engineering ,chemistry ,law ,Specific surface area ,Titanium dioxide ,Solar cell ,Environmental Chemistry ,0210 nano-technology ,Mesoporous material - Abstract
Mesoporous TiO2 spheres with high crystallinity and large specific surface area were facilely prepared via sol–gel and solvothermal methods. By tuning the time and temperature of the solvothermal process, the specific surface area and pore size of TiO2 spheres were fine-controlled without any additives. Compared with the dye-sensitized solar cell (DSC) based on the commercial P25 nanoparticles, the dye-sensitized solar cell employing optimized TiO2 spheres achieves an excellent efficiency of 10.3%, due to the outstanding dye-loading and light-scattering abilities as well as attenuated charge recombination. Furthermore, an impressive efficiency of 11.2% can be obtained after TiCl4 post-treatment. More importantly, in combination with a single-layer TiO2 film composed of mesoporous spheres and low-volatility electrolyte, the DSC exhibits high efficiency and long-term durability.
- Published
- 2018
9. Plasmon-Enhanced Self-Powered UV Photodetectors Assembled by Incorporating Ag@SiO2 Core–Shell Nanoparticles into TiO2 Nanocube Photoanodes
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Dongbo Wang, Qingjiang Yu, Jianan Wang, Y.D. Huang, Zhi Zeng, Shiyong Gao, Shujie Jiao, Jinzhong Wang, James Taban Abdalla, and Cuiling Yu
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Photocurrent ,Materials science ,Renewable Energy, Sustainability and the Environment ,business.industry ,General Chemical Engineering ,Photodetector ,Nanoparticle ,02 engineering and technology ,General Chemistry ,010402 general chemistry ,021001 nanoscience & nanotechnology ,medicine.disease_cause ,01 natural sciences ,0104 chemical sciences ,Responsivity ,Ultrafast laser spectroscopy ,medicine ,Environmental Chemistry ,Optoelectronics ,Surface plasmon resonance ,0210 nano-technology ,business ,Ultraviolet ,Plasmon - Abstract
A novel photoelectrochemical self-powered ultraviolet photodetector (UVPD) has been assembled employing Ag@SiO2 core–shell nanoparticles (NPs) incorporated TiO2 nanocubes (NCs) as the photoanode. The incorporation of the plasmonic core–shell NPs can boost the photocurrent of the self-powered UVPD. Finite difference time domain (FDTD) and transient absorption spectroscopy (TAS) were employed to understand plasmonic enhancement processes. By optimizing the incorporated ratio of Ag@SiO2 NPs, the photocurrent of UVPD with 2 wt % Ag@SiO2 NPs reaches the maximum value in view of the enhanced light harvesting and effective inhibition of charge recombination. More importantly, the UVPD with 2 wt % Ag@SiO2 NPs achieves a high on/off ratio of 8212 and a remarkable responsivity of 0.151 A W–1, combined with a rapid response time, prominent spectral selectivity, and photosensitivity linearity response.
- Published
- 2017
10. Sequencing trait-associated mutations to clone wheat rust-resistance gene YrNAM
- Author
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Fei Ni, Yanyan Zheng, Xiaoke Liu, Yang Yu, Guangqiang Zhang, Lynn Epstein, Xue Mao, Jingzheng Wu, Cuiling Yuan, Bo Lv, Haixia Yu, Jinlong Li, Qi Zhao, Qiyu Yang, Jiajun Liu, Juan Qi, Daolin Fu, and Jiajie Wu
- Subjects
Science - Abstract
Abstract Stripe (yellow) rust, caused by Puccinia striiformis f. sp. tritici (Pst), can significantly affect wheat production. Cloning resistance genes is critical for efficient and effective breeding of stripe rust resistant wheat cultivars. One resistance gene (Yr10 CG ) underlying the Pst resistance locus Yr10 has been cloned. However, following haplotype and linkage analyses indicate the presence of additional Pst resistance gene(s) underlying/near Yr10 locus. Here, we report the cloning of the Pst resistance gene YrNAM in this region using the method of sequencing trait-associated mutations (STAM). YrNAM encodes a non-canonical resistance protein with a NAM domain and a ZnF-BED domain. We show that both domains are required for resistance. Transgenic wheat harboring YrNAM gene driven by its endogenous promoter confers resistance to stripe rust races CYR32 and CYR33. YrNAM is an ancient gene and present in wild wheat species Aegilops longissima and Ae. sharonensis; however, it is absent in most wheat cultivars, which indicates its breeding value.
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- 2023
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11. The Pioneer of Enlightenment
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Cuiling Yu
- Subjects
media_common.quotation_subject ,Philosophy ,Enlightenment ,Classics ,media_common - Published
- 2016
12. Surface Insulation Breakdown Behaviors in Liquid Nitrogen Environment of Epoxy/BN Composite
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Yunqi Xing, Ji Wei Zhang, Jie Li, Hongli Zhang, Meng Xiao, Cuiling Yu, Boxue Du, and Qingquan Li
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010302 applied physics ,Superconductivity ,Materials science ,Infrared ,Composite number ,Epoxy ,Liquid nitrogen ,Condensed Matter Physics ,01 natural sciences ,Surface discharge ,Electronic, Optical and Magnetic Materials ,visual_art ,0103 physical sciences ,Thermal ,visual_art.visual_art_medium ,Electrical and Electronic Engineering ,Composite material ,010306 general physics ,Inhibitory effect - Abstract
Epoxy resin as an insulating material is widely used in superconducting motors because it has excellent insulation properties under extremely low temperature. The insulation properties could be damaged by surface discharge. Our previous studies were focused on surface insulation breakdown behaviors in the room temperature condition, and the results showed that the accumulated heat at the discharge area was the main reason to cause insulation breakdown. The performance of surface discharge erosion resistance in a liquid nitrogen environment was not clear. In this paper, surface insulation breakdown behaviors in liquid nitrogen were studied to clarify the effects of extremely low temperature and BN particles on tracking performance. A digital camera and an infrared camera were used to measure the breakdown process. The camera was used to record the time to breakdown and observe the whole insulation breakdown process, while the thermal accumulation and decay process was measured by the infrared camera. Obtained results indicated that insulation breakdown performance was affected by BN fillers and low temperature significantly. In the liquid nitrogen environment, the time to tracking failure is much longer than that in air. It is concluded that the extremely low temperature in liquid nitrogen could produce an inhibitory effect on surface insulation breakdown.
- Published
- 2016
13. Peanut NAC Transcription Factor AhNAPa Negatively Regulates Salt Tolerance in Transgenic Arabidopsis
- Author
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Cuiling Yuan, Haocui Miao, Quanxi Sun, and Shihua Shan
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AhNAPa ,NAC transcription factor ,salt tolerance ,negative regulator ,peanut ,Agriculture - Abstract
Soil salinity greatly impacts the planting area of cultivated peanut. It is necessary to breed salt-tolerant cultivars. However, few salt-resistant genes have been identified in peanut. Here, we reported the cloning of a peanut NAC transcription factor gene, AhNAPa, which was expressed ubiquitously and significantly upregulated after salt treatment. Furthermore, an AhNAPa-GFP fusion protein was found to be located in the nucleus, which indicated that AhNAPa might perform functions as a transcriptional activator in peanut. Under NaCl treatment, the root length of Arabidopsis plants overexpressing AhNAPa (AhNAPa-OX) were dramatically inhibited compared with the wild type (WT) lines, and the AhNAPa-OX adult plants became sensitive to salt stress. The expression levels of abiotic stress-responsive genes, SnRK2.2, NAC016, RD20, RD29B, and AREB1, significantly decreased in AhNAPa-OX plants, suggesting AhNAPa inhibited the ABA signaling pathway in response to salt stress. Taken together, these results suggest that the salt-inducible peanut transcription factor AhNAPa negatively regulated salt tolerance in transgenic Arabidopsis.
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- 2024
- Full Text
- View/download PDF
14. Heterostructured TiO2/MgO nanowire arrays for self-powered UV photodetectors
- Author
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Shiming Ni, Weijie Lu, Qingjiang Yu, Xitian Zhang, Lin Li, Y.D. Huang, Haigang Wu, Fengyun Guo, Cuiling Yu, and Jianan Wang
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Photocurrent ,Signal response ,Materials science ,business.industry ,General Chemical Engineering ,Nanowire ,Photodetector ,02 engineering and technology ,General Chemistry ,010402 general chemistry ,021001 nanoscience & nanotechnology ,Tin oxide ,01 natural sciences ,0104 chemical sciences ,Responsivity ,Optoelectronics ,0210 nano-technology ,business ,Light irradiance ,Layer (electronics) - Abstract
Heterostructured TiO2/MgO nanowire arrays (NWAs) were grown vertically on fluorine-doped tin oxide (FTO) glass by a simple solvothermal method. A photoelectrochemical self-powered UV photodetector (UVPD) was fabricated using the heterostructured TiO2/MgO nanowire arrays (NWAs) as the photoanode. The TiO2/MgO NWAs based UVPDs shows a higher photocurrent density (J) and open-circuit voltage (Voc) with respect to the UVPD with bare TiO2 NWAs under UV light irradiance. The intrinsic role of the MgO shell layer on the J and Voc varitions of UVPDs is investigated in detail. An impressive responsivity of 0.233 A W−1 was achieved for the UVPD with TiO2/MgO NWAs, which is the highest responsivity for photoelectrochemical self-powered UVPDs based on various TiO2 nanostructured photoanodes so far. Moreover, this UVPD also exhibits a high on/off ratio, fast response time, excellent visible-blind characteristic and linear optical signal response.
- Published
- 2016
15. A high-performance self-powered UV photodetector based on SnO2 mesoporous spheres @ TiO2
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Cuiling Yu, Jinzhong Wang, Xiaochao Li, Qingjiang Yu, Yuan Yan, Feifei Shi, Dongbo Wang, Y.D. Huang, and Shiyong Gao
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Photocurrent ,Materials science ,Tin dioxide ,business.industry ,Analytical chemistry ,Photodetector ,Photoelectrochemical cell ,Electronic, Optical and Magnetic Materials ,Dielectric spectroscopy ,chemistry.chemical_compound ,chemistry ,Optoelectronics ,Quantum efficiency ,Absorption (electromagnetic radiation) ,Mesoporous material ,business - Abstract
A novel self-powered UV photodetector (UVPD) based on the photoelectrochemical cell (PECC) has been constructed using the TiO2 coated SnO2 mesoporous spheres (SnO2-MS@TiO2). This self-powered UVPD displays a higher photocurrent density compared to the UVPD with the pure SnO2-MS. By means of external quantum efficiency (EQE), UV-vis absorption, and electrochemical impedance measurements, we scrutinize the intrinsic role of the TiO2 coating layer on the photocurrent enhancement. Under UV irradiation, this UVPD exhibits a high on/off ratio of 11519, a fast rise time of 0.007 s and decay time of 0.006 s, together with the excellent visible-blind characteristic and linear optical signal response. The self-powered photodetector is a promising candidate for application in high-sensitivity and high-speed UVPDs.
- Published
- 2015
16. Rapid protocol for in vitro induction of cytokine associated with Th2 and Th17 cells from PBMC
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Hortensia Soto, Jeanne Elia, Cuiling Yu, Daniel Yu, Jie Yu, and Jessie Ni
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Immunology ,Immunology and Allergy - Abstract
T helper cell is a key component of the immune system. They mediate humoral and cellular immune responses against pathogens and other diseases like cancer by inducing and activating effector cells such as B cells, cytotoxic T cells and innate cells. They are also involved in the regulation of immune responses by controlling the persistence and magnitude of the response. Activation of naïve T cell is initiated by interaction with antigen presenting cells. The downstream signal transduction and the cytokine milieu can lead to their proliferation and differentiation into different T cell subtypes. To facilitate the study of Th2 and Th17 cells, we present a rapid protocol to efficiently induce cytokine associated with Th2 and Th17 cells from total PBMC. Canonical anti-CD3 and anti-CD28 clones are used to activate the purified cells. Different sets of bioassay-validated cytokines, including IL-12, IL-4, IL-6, IL-1β, IL-2, and TGF-β1 are used to induce cytokine associated with Th2 and Th17 cells. Hallmark cytokine and transcription factor expression profile of different lineages are evaluated by Flow Cytometry. In summary, we have successfully developed a rapid protocols to induce cytokine expression in vitro associated with Th2 and Th17 using high quality reagents developed in BioLegend.
- Published
- 2020
17. Zinc-doped SnO2 nanocrystals as photoanode materials for highly efficient dye-sensitized solar cells
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Jinzhong Wang, Renzhi Li, Liancheng Zhao, Qingjiang Yu, Fengyun Guo, Y.D. Huang, Xiaochao Li, Cuiling Yu, Yong Zhang, and Shiyong Gao
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Materials science ,Renewable Energy, Sustainability and the Environment ,Energy conversion efficiency ,Doping ,chemistry.chemical_element ,Nanotechnology ,General Chemistry ,Zinc ,Hydrothermal circulation ,Negative shift ,Dye-sensitized solar cell ,chemistry ,Chemical engineering ,Nanocrystal ,General Materials Science ,Zn doping - Abstract
Zn-doped SnO2 nanocrystals were successfully synthesized by a simple hydrothermal method. It is found that Zn doping into SnO2 can induce a negative shift in the flat-band potential (VFB) and increase the isoelectric point. As a result, dye-sensitized solar cells (DSCs) based on Zn-doped SnO2 nanocrystal photoanodes exhibit longer electron lifetimes and higher dye loading compared to undoped SnO2 based DSCs. The overall power conversion efficiency (η) of the optimized Zn-doped SnO2 based DSC reaches 4.18% and increases to 7.70% after the TiCl4 treatment. More importantly, a remarkable η of 8.23% is achieved for DSCs based on a high-quality double-layer SnO2 photoanode with the TiCl4 treatment, to the best of our knowledge, which is so far the best reported efficiency for DSCs based on SnO2 photoanodes.
- Published
- 2015
18. One-pot synthesis of hierarchical SnO2 hollow nanospindles self-assembled from nanorods and their lithium storage properties
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Jinzhong Wang, Qingjiang Yu, Huixin Wang, Xuan-zhang Wang, Xitian Zhang, Fengyun Guo, Cuiling Yu, Wenqi Wang, Shiyong Gao, Y.D. Huang, and Hong Gao
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Nanostructure ,Materials science ,General Chemical Engineering ,One-pot synthesis ,Nucleation ,chemistry.chemical_element ,Nanoparticle ,Nanotechnology ,General Chemistry ,Electrochemistry ,Hydrothermal circulation ,chemistry ,Lithium ,Nanorod - Abstract
Novel hierarchical SnO2 hollow nanospindles self-assembled from nanorods have been successfully synthesized via a templating approach under hydrothermal conditions. The influence of the reactant concentration on SnO2 products is investigated in detail. It is found that the interplay of the acidic etching of Fe2O3 templates and controlled hydrolysis of SnCl2 is significant for the formation of the hierarchical SnO2 hollow nanostructures. The evolution process and formation mechanism of the hollow structures with nanorods are analyzed from the angle of nucleation and morphology. Moreover, the electrochemical properties of the hollow structures are also studied by charge–discharge cycling, the result displays that the hierarchical SnO2 hollow nanostructure exhibits much better lithium storage properties with higher reversible capacities and enhanced cyclic capacity retention than the commercial SnO2 nanoparticles.
- Published
- 2015
19. Genome-Wide Identification of Peanut B-Boxs and Functional Characterization of AhBBX6 in Salt and Drought Stresses
- Author
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Haohong Tang, Cuiling Yuan, Haonan Shi, Feng Liu, Shihua Shan, Zhijun Wang, Quanxi Sun, and Jie Sun
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peanut ,BBX gene family ,abiotic stress ,VIGS ,Botany ,QK1-989 - Abstract
The B-box (BBX) gene family includes zinc finger protein transcription factors that regulate a multitude of physiological and developmental processes in plants. While BBX gene families have been previously determined in various plants, the members and roles of peanut BBXs are largely unknown. In this research, on the basis of the genome-wide identification of BBXs in three peanut species (Arachis hypogaea, A. duranensis, and A. ipaensis), we investigated the expression profile of the BBXs in various tissues and in response to salt and drought stresses and selected AhBBX6 for functional characterization. We identified a total of 77 BBXs in peanuts, which could be grouped into five subfamilies, with the genes from the same branch of the same subgroup having comparable exon–intron structures. In addition, a significant number of cis-regulatory elements involved in the regulation of responses to light and hormones and abiotic stresses were found in the promoter region of peanut BBXs. Based on the analysis of transcriptome data and qRT-PCR, we identified AhBBX6, AhBBX11, AhBBX13, and AhBBX38 as potential genes associated with tolerance to salt and drought. Silencing AhBBX6 using virus-induced gene silencing compromised the tolerance of peanut plants to salt and drought stresses. The results of this study provide knowledge on peanut BBXs and establish a foundation for future research into their functional roles in peanut development and stress response.
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- 2024
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20. Hsf transcription factor gene family in peanut (Arachis hypogaea L.): genome-wide characterization and expression analysis under drought and salt stresses
- Author
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Qi Wang, Zhenbiao Zhang, Cun Guo, Xiaobo Zhao, Zhiyuan Li, Yifei Mou, Quanxi Sun, Juan Wang, Cuiling Yuan, Chunjuan Li, Ping Cong, and Shihua Shan
- Subjects
peanut ,Hsf ,abiotic stress ,transcription factor ,gene family ,Plant culture ,SB1-1110 - Abstract
Heat shock transcription factors (Hsfs) play important roles in plant developmental regulations and various stress responses. In present study, 46 Hsf genes in peanut (AhHsf) were identified and analyzed. The 46 AhHsf genes were classed into three groups (A, B, and C) and 14 subgroups (A1-A9, B1-B4, and C1) together with their Arabidopsis homologs according to phylogenetic analyses, and 46 AhHsf genes unequally located on 17 chromosomes. Gene structure and protein motif analysis revealed that members from the same subgroup possessed similar exon/intron and motif organization, further supporting the results of phylogenetic analyses. Gene duplication events were found in peanut Hsf gene family via syntenic analysis, which were important in Hsf gene family expansion in peanut. The expression of AhHsf genes were detected in different tissues using published data, implying that AhHsf genes may differ in function. In addition, several AhHsf genes (AhHsf5, AhHsf11, AhHsf20, AhHsf24, AhHsf30, AhHsf35) were induced by drought and salt stresses. Furthermore, the stress-induced member AhHsf20 was found to be located in nucleus. Notably, overexpression of AhHsf20 was able to enhance salt tolerance. These results from this study may provide valuable information for further functional analysis of peanut Hsf genes.
- Published
- 2023
- Full Text
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21. Ultralong Rutile TiO2 Nanowire Arrays for Highly Efficient Dye-Sensitized Solar Cells
- Author
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Xitian Zhang, Peng Wang, Y.D. Huang, Jinzhong Wang, Renzhi Li, Yong Zhang, Liancheng Zhao, Cuiling Yu, Qingjiang Yu, Shiyong Gao, Shujie Jiao, Hailiang Li, and Fengyun Guo
- Subjects
Materials science ,Solvothermal synthesis ,Energy conversion efficiency ,Nanowire ,Nanotechnology ,02 engineering and technology ,010402 general chemistry ,021001 nanoscience & nanotechnology ,Tin oxide ,01 natural sciences ,0104 chemical sciences ,Dye-sensitized solar cell ,Chemical engineering ,Rutile ,General Materials Science ,0210 nano-technology ,Layer (electronics) ,Electrical conductor - Abstract
Vertically aligned rutile TiO2 nanowire arrays (NWAs) with lengths of ∼44 μm have been successfully synthesized on transparent, conductive fluorine-doped tin oxide (FTO) glass by a facile one-step solvothermal method. The length and wire-to-wire distance of NWAs can be controlled by adjusting the ethanol content in the reaction solution. By employing optimized rutile TiO2 NWAs for dye-sensitized solar cells (DSCs), a remarkable power conversion efficiency (PCE) of 8.9% is achieved. Moreover, in combination with a light-scattering layer, the performance of a rutile TiO2 NWAs based DSC can be further enhanced, reaching an impressive PCE of 9.6%, which is the highest efficiency for rutile TiO2 NWA based DSCs so far.
- Published
- 2016
22. Identification of human plasma cells with a lamprey monoclonal antibody
- Author
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Jiefei Tong, Wei Zhan, Mario A. Ostrowski, Paolo Campisi, Brantley R. Herrin, Götz R. A. Ehrhardt, David L. Jaye, Suzanne Trudel, F. Eun-Hyung Lee, Evan J. Propst, Max D. Cooper, Ignacio Sanz, Michael F. Moran, Mengyao Shi, Justin Chan, Shuya Kyu, Cuiling Yu, Srijit Khan, Dariush Davani, Yanling Liu, Marion J. Parsons, Eyal Grunebaum, and Zhihua Li
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0301 basic medicine ,biology ,medicine.drug_class ,Lamprey ,Immunology ,lcsh:R ,lcsh:Medicine ,General Medicine ,CD38 ,medicine.disease ,Monoclonal antibody ,biology.organism_classification ,Epitope ,3. Good health ,03 medical and health sciences ,030104 developmental biology ,Technical Advance ,Variable lymphocyte receptor ,Antigen ,immune system diseases ,Monoclonal ,medicine ,Multiple myeloma - Abstract
Ab-producing plasma cells (PCs) serve as key participants in countering pathogenic challenges as well as being contributors to autoimmune and malignant disorders. Thus far, only a limited number of PC–specific markers have been identified. The characterization of the unique variable lymphocyte receptor (VLR) Abs that are made by evolutionarily distant jawless vertebrates prompted us to investigate whether VLR Abs could detect novel PC antigens that have not been recognized by conventional Abs. Here, we describe a monoclonal lamprey Ab, VLRB MM3, that was raised against primary multiple myeloma cells. VLRB MM3 recognizes a unique epitope of the CD38 ectoenzyme that is present on plasmablasts and PCs from healthy individuals and on most, but not all, multiple myelomas. Binding by the VLRB MM3 Ab coincides with CD38 dimerization and NAD glycohydrolase activity. Our data demonstrate that the lamprey VLRB MM3 Ab is a unique reagent for the identification of plasmablasts and PCs, with potential applications in the diagnosis and therapeutic intervention of PC or autoimmune disorders.
- Published
- 2016
23. Phase transformation and resistivity of dumbbell-like ZnO microcrystals under high pressure
- Author
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Cuiling Yu, Qingjiang Yu, Chunxiao Gao, Haibin Yang, Gang Peng, Bao Liu, Yonghao Han, Dongmei Zhang, Xiaoyan Cui, Cailong Liu, Yue Wang, Baojia Wu, Chunyuan He, Xiaowei Huang, and Guangtian Zou
- Subjects
Phase transformations (Statistical physics) -- Analysis ,Raman effect -- Analysis ,Zinc oxide -- Thermal properties ,Zinc oxide -- Structure ,Physics - Abstract
The pressure-induced phase-transition properties of the dumbbell-like ZnO microcrystals are analyzed by using pressure-dependent Raman scattering. The pressure dependence of the electrical resistivity for the dumbbell-like ZnO microcrystals annealed at different conditions is examined.
- Published
- 2008
24. Growth of dumbbell-like ZnO microcrystals under mild conditions and their photoluminescence properties
- Author
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Qingjiang Yu, Cuiling Yu, Haibin Yang, Wuyou Fu, Lianxia Chang, Jing Wu, Ronghui Wei, Hongdong Li, Hongyang Zhu, Minghui Li, Guangtian Zou, Guorui Wang, Changlu Shao, and Yichun Liu
- Subjects
Binding energy -- Research ,Photoluminescence -- Analysis ,Scanning electron microscopes -- Observations ,Zinc oxide -- Structure ,Zinc oxide -- Electric properties ,Zinc oxide -- Optical properties ,Chemistry - Abstract
A facile solution method is used for preparing large-scale uniform dumbbell-like ZnO microcrystals under mild conditions and the influence of the reactant concentration on the size and shapes of the ZnO samples are analyzed. The room-temperature photoluminescence (PL) spectrum of the dumbbell-like ZnO microcrystals have displayed a strong ultraviolet (UV) emission peak, which is originated from the radiative free-exciton recombination by the temperature-dependent PL.
- Published
- 2007
25. Synthesis, Optical Properties, and Ethanol-Sensing Properties of Bicone-like ZnO Microcrystals via a Simple Solution Method
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Cuiling Yu, Guangtian Zou, Mingxia Yuan, Shikai Liu, Qingjiang Yu, Wuyou Fu, Haibin Yang, Jin Guo, and Minghui Li
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SIMPLE (dark matter experiment) ,Photoluminescence ,Ethanol ,Materials science ,Exciton ,Nanotechnology ,Surfaces, Coatings and Films ,Electronic, Optical and Magnetic Materials ,Bicone ,Characterization (materials science) ,chemistry.chemical_compound ,General Energy ,chemistry ,Chemical engineering ,Radiative transfer ,Physical and Theoretical Chemistry ,Spectroscopy - Abstract
Large-scale uniform bicone-like ZnO microcrystals were successfully synthesized via a simple solution method at lower temperature (80 °C). The as-prepared bicones, composed of two cones, possess a single-crystal hexagonal structure and grow along the [0001] direction. The influence of the reactant concentration on the size and shape of the ZnO samples has been studied in detail, and the results revealed that reactant concentration plays a crucial role in determining the final morphologies of the samples. The growth process of the bicone-like ZnO microcrystals was viewed by field emission scanning electron microscopy (FESEM) characterization, and a possible formation mechanism was proposed. Optical properties of the bicone-like ZnO microcrystals were also investigated by photoluminescence (PL) spectroscopy. The room-temperature PL spectrum of the bicone-like ZnO microcrystals shows a strong UV emission peak. The UV emission is further identified to originate from the radiative free exciton recombination by...
- Published
- 2009
26. Inhibitory signaling potential of a TCR-like molecule in lamprey
- Author
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Anlong Xu, Matthew N. Alder, Cuiling Yu, Götz R. A. Ehrhardt, and Max D. Cooper
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MAPK/ERK pathway ,Immunology ,T-cell receptor ,Tyrosine phosphorylation ,Biology ,Molecular biology ,Fusion protein ,Receptor tyrosine kinase ,Cell biology ,chemistry.chemical_compound ,chemistry ,biology.protein ,Immunology and Allergy ,Signal transduction ,Tyrosine ,Receptor - Abstract
A TCR-like molecule (TCRL) with two canonical ITIM has been identified in the sea lamprey. We show here that TCRL is preferentially expressed by lymphocytes bearing variable lymphocyte receptors. To examine the potential of the TCRL inhibitory motifs, chimeric proteins comprising the FccRIIb extracellular and transmembrane domains and the TCRL intracellular domain were expressed in a mouse B-cell line. BCR co-ligation with the WT version of the FccRIIb/TCRL chimeric protein resulted in its tyrosine phosphorylation and the inhibition of BCR-induced calcium mobilization, whole-cell protein tyrosine phosphorylation and Erk/Akt/JNK activation. Tyrosine to phenylalanine mutations in either or both ITIM compromised the inhibitory capacity of this receptor chimera. Analysis of receptor-associated proteins indicated that the inhibition is mediated by recruitment of the protein tyrosine kinases, SHP1 and SHP2. These findings demonstrate the inhibitory potential of TCRL and its expression by clonally diverse lymphocytes bearing the variable lymphocyte receptors, thereby implying an immunomodulatory role for this ancestral TCR relative in a jawless vertebrate.
- Published
- 2009
27. Genomic analysis of the immune gene repertoire of amphioxus reveals extraordinary innate complexity and diversity
- Author
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Huiqing Huang, Tao Wu, Kui Wu, Yingcai Yu, Huiling Liu, Shangwu Chen, Jin Ge, Meiling Dong, Shengfeng Huang, Jun Li, Tong Liu, Shaochun Yuan, Anlong Xu, Cuiling Yu, Yanhong Yu, Manyi Yang, and Lei Guo
- Subjects
Letter ,animal structures ,Molecular Sequence Data ,Chordate ,Biology ,Genome ,Mice ,Immune system ,Species Specificity ,Chordata, Nonvertebrate ,biology.animal ,Branchiostoma floridae ,Genetics ,Animals ,Humans ,Immunologic Factors ,Receptors, Immunologic ,Zebrafish ,Genetics (clinical) ,Innate immune system ,Genetic Variation ,Lampreys ,Vertebrate ,Sequence Analysis, DNA ,Acquired immune system ,biology.organism_classification ,Immunity, Innate ,Drosophila melanogaster ,Evolutionary biology ,Sea Urchins ,C1q domain - Abstract
It has been speculated that before vertebrates evolved somatic diversity-based adaptive immunity, the germline-encoded diversity of innate immunity may have been more developed. Amphioxus occupies the basal position of the chordate phylum and hence is an important reference to the evolution of vertebrate immunity. Here we report the first comprehensive genomic survey of the immune gene repertoire of the amphioxus Branchiostoma floridae. It has been reported that the purple sea urchin has a vastly expanded innate receptor repertoire not previously seen in other species, which includes 222 toll-like receptors (TLRs), 203 NOD/NALP-like receptors (NLRs), and 218 scavenger receptors (SRs). We discovered that the amphioxus genome contains comparable expansion with 71 TLR gene models, 118 NLR models, and 270 SR models. Amphioxus also expands other receptor-like families, including 1215 C-type lectin models, 240 LRR and IGcam-containing models, 1363 other LRR-containing models, 75 C1q-like models, 98 ficolin-like models, and hundreds of models containing complement-related domains. The expansion is not restricted to receptors but is likely to extend to intermediate signal transducers because there are 58 TIR adapter-like models, 36 TRAF models, 44 initiator caspase models, and 541 death-fold domain-containing models in the genome. Amphioxus also has a sophisticated TNF system and a complicated complement system not previously seen in other invertebrates. Besides the increase of gene number, domain combinations of immune proteins are also increased. Altogether, this survey suggests that the amphioxus, a species without vertebrate-type adaptive immunity, holds extraordinary innate complexity and diversity.
- Published
- 2008
28. Absorptive capacity of information technology and its conceptual model
- Author
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Xinhua Bi and Cuiling Yu
- Subjects
Engineering ,Multidisciplinary ,Knowledge management ,business.industry ,Process (engineering) ,media_common.quotation_subject ,Information technology ,System dynamics ,Identification (information) ,Absorptive capacity ,Order (exchange) ,Conceptual model ,business ,Adaptation (computer science) ,media_common - Abstract
In order to examine the problem of how to improve the use of information technology (IT) in enterprises, this paper makes an exploration from the perspective of organizational absorptive capacity. We propose the concept of IT absorptive capacity from an organizational level. A dynamic process model is developed to further analyze IT absorption. IT absorptive capacity of this process is embodied as six forms: identification, adoption, adaptation, acceptance, infusion, and knowledge management. By means of questionnaire surveys of 76 Chinese enterprises, the main factors that favor or disable the capacity of each stage are discovered. Using the method of system dynamics, a conceptual model of IT absorptive capacity is developed to analyze the action mechanism of the factors in detail. The model indicates that the critical factors are embodied in the aspect of management. Furthermore, it demonstrates that IT absorption is a spiral process, during which IT absorptive capacity evolves dynamically and, consequently, promotes IT use.
- Published
- 2008
29. Structural, electrical and optical properties of yttrium-doped ZnO thin films prepared by sol–gel method
- Author
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Shikai Liu, Guangtian Zou, Guorui Wang, Wuyou Fu, Cuiling Yu, Ronghui Wei, Yongming Sui, Qingjiang Yu, Yichun Liu, Zhanlian Liu, Changlu Shao, Minghui Li, and Haibin Yang
- Subjects
Photoluminescence ,Materials science ,Acoustics and Ultrasonics ,business.industry ,Annealing (metallurgy) ,Reducing atmosphere ,Doping ,Analytical chemistry ,chemistry.chemical_element ,Yttrium ,Condensed Matter Physics ,Surfaces, Coatings and Films ,Electronic, Optical and Magnetic Materials ,Optics ,chemistry ,Electrical resistivity and conductivity ,Thin film ,business ,Sol-gel - Abstract
Yttrium-doped ZnO thin films were deposited on silica glass substrates by the sol–gel method. The structural, electrical and optical properties of yttrium-doped ZnO thin films were investigated systematically and in detail. All the thin films have a preferred (0 0 2) orientation. When compared with the electrical resistivity values of films without annealing treatment, the values of films annealed in the reducing atmosphere were decreased by about three orders of magnitude. The lowest electrical resistivity value was 6.75 × 10−3 Ω cm, which was obtained in the 0.5 at% yttrium-doped ZnO thin film annealed in nitrogen with 5% hydrogen at 500 °C. In room-temperature photoluminescence (PL) spectra, two PL emission peaks are found in the pure ZnO thin film; one is the near-band-edge (NBE) emission at 3.22 eV and the other is a green emission at about 2.38 eV. Nevertheless, the green emission is not found in the PL of the yttrium-doped ZnO thin films. The low-temperature PL spectrum of the undoped ZnO thin film at 83 K is split into well-resolved free and bound excition emission peaks in the ultraviolet region, but the NBE emission of the 5 at% yttrium-doped ZnO thin film at 83 K has only one broad emission peak.
- Published
- 2007
30. Transparent conducting yttrium-doped ZnO thin films deposited by sol–gel method
- Author
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Guangtian Zou, Cuiling Yu, Lianxia Chang, Wuyou Fu, Qingjiang Yu, Kai Du, Ronghui Wei, Hongyang Zhu, Minghui Li, Haibin Yang, and Jing Xu
- Subjects
Materials science ,Dopant ,Band gap ,Annealing (metallurgy) ,Reducing atmosphere ,Doping ,Metals and Alloys ,Analytical chemistry ,chemistry.chemical_element ,Mineralogy ,Surfaces and Interfaces ,Yttrium ,Surfaces, Coatings and Films ,Electronic, Optical and Magnetic Materials ,chemistry ,Materials Chemistry ,Thin film ,Sol-gel - Abstract
Transparent and conductive high preferential c-axis oriented ZnO thin films doped with yttrium have been prepared by sol–gel method using zinc acetate and yttrium chloride as cations source, 2-methoxyethanol as solvent and monoethanolamine (MEA) as sol stabilizer. Film deposition was performed by dip-coating technique at a withdrawal rate of 6.0 cm/min on silica glass substrates. The effect of dopant (Y) concentration, heating treatment and annealing in a reducing atmosphere on the structure, morphology, electrical and optical properties of ZnO thin films were investigated. When compared with the resistivity values of films without the annealing treatment, the values of films annealed in the reducing atmosphere were decreased by about three orders of magnitude. The lowest resistivity value was 6.75 × 10− 3 Ω cm, which was obtained in the 0.5 at.% yttrium-doped ZnO thin film annealed in nitrogen with 5% hydrogen at 500 °C. The average optical transmittance values of the annealed films were more than 80% in the visible range. The energy band gap calculated from the transmittance spectra is about 3.30–3.37 eV.
- Published
- 2007
31. Hydrocarbon migration along the Shengbei fault zone in the Bohai Bay basin, China: The evidence from geochemistry and fluid inclusions
- Author
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Cuiling Yu and Jianhui Zeng
- Subjects
chemistry.chemical_classification ,geography ,geography.geographical_feature_category ,Geochemistry ,engineering.material ,Structural basin ,Fault (geology) ,Hydrocarbon ,Source rock ,chemistry ,Geochemistry and Petrology ,Illite ,engineering ,Fluid dynamics ,Economic Geology ,Fluid inclusions ,Petrology ,Clay minerals ,human activities ,Geology - Abstract
Faults are important pathways for fluid and hydrocarbon migration, and they play a crucial role in oil and gas migration and accumulation. But until now, the acquisition of evidence on fluid flow along a fault is still a puzzle. Based on studies of clay minerals, oil geochemistry and fluid inclusions in Shengbei fault zone, we find that: 1) distribution of illite is abnormal; 2) oil in the Sha-2 Member of the Shahejie Formation (Es2) and its overlying intervals was derived from the deep lower source rocks, i.e. the Sha-3 and Sha-4 Members of the Shahejie Formation (Es3 and Es4); and 3) fluid inclusions adjacent to F1 fault in the Es4 have mixed features of Es3 and Es4. These findings indicate that fluid and hydrocarbon migration (both vertical and lateral migration) once occurred along the F1 fault, that is, this fault once acted as a pathway for fluid and hydrocarbon migration.
- Published
- 2006
32. Cloning and functional analysis of the promoter of allergen gene Ara h 1 from peanut
- Author
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Cuiling Yuan, Chunjuan Li, Caixia Yan, Xiaobo Zhao, Juan Wang, Yifei Mou, Zhiwei Wang, Quanxi Sun, and Shihua Shan
- Subjects
Seed-specific promoter ,Allergen-encoding gene ,Peanut ,GUS histochemical analysis ,Transgenic Arabidopsis ,Genetics ,QH426-470 - Abstract
Peanut seeds are ideal bioreactors for the production of foreign recombinant proteins or secondary metabolites. Seed-specific promoters (SSPs) can direct the expression of genes specifically in seeds to avoid undesirable effects associated with constitutive expression. However, few SSPs have been identified in peanut. Previous studies have shown that some allergen-encoding genes encode seed storage proteins or exhibit seed-specific/preferential expression. In this study, we characterized allergen-encoding genes from across the genomes of Arachis species to explore seed-specific genes. We found that at least 9 out of 16 identified peanut allergen-encoding genes were expressed specifically in the seeds or were preferentially expressed. A 1493-bp promoter fragment of allergen gene Ara h 1 (we named it AHSSP6) was isolated from cultivated peanut genome. cis-element analysis showed that three RY repeat elements which usually exsisted in seed or embryo specific promoter sequence were also present in AHSSP6 sequence. Histochemical analysis showed AHSSP6 could drive the expression of a β-glucuronidase (GUS) reporter gene specifically in the seeds or cotyledon tissue of transgenic Arabidopsis, while not in other tissues. These findings indicated that these promoters of allergen genes were candidate SSPs, and AHSSP6 was a novel SSP which could be potentially utilized in peanut improvement.
- Published
- 2022
- Full Text
- View/download PDF
33. Identification and characterisation of a homolog of an activation gene for the recombination activating gene 1 (RAG 1) in amphioxus
- Author
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Anlong Xu, Xiaokun Wu, Shaochun Yuan, Jianwen Wei, Cuiling Yu, Yonggui Fu, Meiling Dong, Yang Shen, Shengfeng Huang, and Jing Su
- Subjects
China ,DNA, Complementary ,Sequence analysis ,Genes, RAG-1 ,Molecular Sequence Data ,Sequence alignment ,Aquatic Science ,Biology ,Recombination-activating gene ,Chordata, Nonvertebrate ,Complementary DNA ,Genes, Regulator ,Animals ,Cluster Analysis ,Environmental Chemistry ,Amino Acid Sequence ,Gene ,Peptide sequence ,Phylogeny ,DNA Primers ,Gene Library ,Regulation of gene expression ,Base Sequence ,Reverse Transcriptase Polymerase Chain Reaction ,cDNA library ,Membrane Proteins ,Sequence Analysis, DNA ,General Medicine ,Molecular biology ,Protein Structure, Tertiary ,Blotting, Southern ,Gene Expression Regulation ,Antibody Formation ,Sequence Alignment ,Signal Transduction - Abstract
Expression of recombination activating genes (RAG) involved in the V (D) J recombination is regulated by the RAG1 gene activator (RGA) in mammals. The sequence of a cDNA clone from an amphioxus cDNA library was found to be homologous to that of RGA from mouse stromal cells with 45% identity. The full-length cDNA sequence comprises 1119 bp and encodes a putative protein of 210 amino acid residues. Characterisation of the amino acid sequence revealed that two MtN3 domains and seven transmembrane spans are present in this protein, indicating a potential role as a plasma membrane protein. This gene is expressed in many tissues and at differential developmental stages. A high expression level of RGA is detected in gonad tissues, and gastrula embryo and adult stages. The presence of the RGA gene in amphioxus suggests that the signal pathway required for the expression of RAG could exist in this primitive protochordate. It also implies that in the related molecules, primitive adaptive immunity may have existed in cephalochordate although the complete machinery of VDJ rearrangement may not be formed.
- Published
- 2005
34. Genes 'Waiting' for Recruitment by the Adaptive Immune System: The Insights from Amphioxus
- Author
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Meiling Dong, Jianwen Wei, Shaochun Yuan, Cuiling Yu, Jing Su, Xuanxian Peng, Shengfeng Huang, Chunyan Mou, Jingchun Du, Shengguo Li, Mengqing Xiang, Anlong Xu, Shicui Zhang, Yanhong Yu, Yang Shen, Xiaojin Xie, Xuesong Yu, Xiaokun Wu, and Jianghai Lin
- Subjects
DNA, Complementary ,Molecular Sequence Data ,Immunology ,ved/biology.organism_classification_rank.species ,Chordate ,Major histocompatibility complex ,Evolution, Molecular ,Species Specificity ,Chordata, Nonvertebrate ,Phylogenetics ,biology.animal ,Animals ,Immunology and Allergy ,Amino Acid Sequence ,Model organism ,Gene ,Phylogeny ,Gene Library ,Expressed Sequence Tags ,Antigen Presentation ,Expressed sequence tag ,Genes, Immunoglobulin ,Sequence Homology, Amino Acid ,biology ,ved/biology ,Proteins ,Vertebrate ,Anatomy ,biology.organism_classification ,Acquired immune system ,Evolutionary biology ,biology.protein - Abstract
In seeking evidence of the existence of adaptive immune system (AIS) in ancient chordate, cDNA clones of six libraries from a protochordate, the Chinese amphioxus, were sequenced. Although the key molecules such as TCR, MHC, Ig, and RAG in AIS have not been identified from our database, we demonstrated in this study the extensive molecular evidence for the presence of genes homologous to many genes that are involved in AIS directly or indirectly, including some of which may represent the putative precursors of vertebrate AIS-related genes. The comparative analyses of these genes in different model organisms revealed the different fates of these genes during evolution. Their gene expression pattern suggested that the primitive digestive system is the pivotal place of the origin and evolution of the AIS. Our studies support the general statement that AIS appears after the jawless/jawed vertebrate split. However our study further reveals the fact that AIS is in its twilight in amphioxus and the evolution of the molecules in amphioxus are waiting for recruitment by the emergence of AIS.
- Published
- 2005
35. Macrophage migration inhibitory factor (MIF) in chinese amphioxus as a molecular marker of immune evolution during the transition of invertebrate/vertebrate
- Author
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Huiping Chen, Jing Su, Jingchun Du, Xiaojin Xie, Wenli Yang, Anlong Xu, Shicui Zhang, Cuiling Yu, Meiling Dong, and Yong-Hua Wang
- Subjects
animal diseases ,Molecular Sequence Data ,Immunology ,chemical and pharmacologic phenomena ,Sequence alignment ,Biology ,Genome ,Mice ,chemistry.chemical_compound ,Chordata, Nonvertebrate ,Complementary DNA ,Molecular marker ,Gene duplication ,otorhinolaryngologic diseases ,Animals ,Humans ,Amino Acid Sequence ,Macrophage Migration-Inhibitory Factors ,Gene ,Expressed Sequence Tags ,Genetics ,Innate immune system ,Base Sequence ,respiratory system ,Biological Evolution ,biological factors ,Rats ,Blotting, Southern ,chemistry ,Macrophage migration inhibitory factor ,Sequence Alignment ,Biomarkers ,Developmental Biology - Abstract
Macrophage migration inhibitory factor (MIF) is an important cytokine related to host defenses and autoimmune diseases. Here, we reported two full-length cDNA clones isolated from Chinese amphioxus (Branchiostoma belcheri tsingtaunese). Amino acid sequences analysis and structure prediction of these two molecules, called Bbt-MIF-I and Bbt-MIF-II, respectively, indicated that several conservative domains existed in the two amphioxus MIFs and their sequences were highly homologous to their counterparts of other species. Intriguingly, the Bbt-MIFs gene is present in multi-copy per haploid genome, which is very unusual compared with vertebrate's MIF gene given the known genome duplication theory. The genomic copy number, expression pattern of MIF gene and phylogenetic analysis of MIF proteins all suggested that a leap forward happened for MIF gene during the evolution from invertebrate to vertebrate. Considering the crucial role of MIF in innate immunity, MIF might serve as one of key molecular markers of evolution of immune system.
- Published
- 2004
36. The bHLH transcription factor AhbHLH112 improves the drought tolerance of peanut
- Author
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Chunjuan Li, Caixia Yan, Quanxi Sun, Juan Wang, Cuiling Yuan, Yifei Mou, Shihua Shan, and Xiaobo Zhao
- Subjects
Basic helix–loop–helix transcription factors ,Peanut ,Drought stress ,Transcriptional regulation ,ROS homeostasis ,Botany ,QK1-989 - Abstract
Abstract Background Basic helix-loop-helix (bHLH) transcription factors (TFs) are one of the largest gene families in plants. They regulate gene expression through interactions with specific motifs in target genes. bHLH TFs are not only universally involved in plant growth but also play an important role in plant responses to abiotic stress. However, most members of this family have not been functionally characterized. Results Here, we characterized the function of a bHLH TF in the peanut, AhHLH112, in response to drought stress. AhHLH112 is localized in the nucleus and it was induced by drought stress. The overexpression of this gene improves the drought tolerance of transgenic plants both in seedling and adult stages. Compared to wild-type plants, the transgenic plants accumulated less reactive oxygen species (ROS), accompanied by increased activity and transcript levels of antioxidant enzymes (superoxide dismutase, peroxidase and catalase). In addition, the WT plants demonstrated higher MDA concentration levels and higher water loss rate than the transgenic plants under drought treatment. The Yeast one-hybrid result also demonstrates that AhbHLH112 directly and specifically binds to and activates the promoter of the peroxidase (POD) gene. Besides, overexpression of AhHLH112 improved ABA level under drought condition, and elevated the expression of genes associated with ABA biosynthesis and ABA responding, including AtNCED3 and AtRD29A. Conclusions Drawing on the results of our experiments, we propose that, by improving ROS-scavenging ability, at least in part through the regulation of POD -mediated H2O2 homeostasis, and possibly participates in ABA-dependent stress-responding pathway, AhbHLH112 acts as a positive factor in drought stress tolerance.
- Published
- 2021
- Full Text
- View/download PDF
37. MIKC-type MADS-box transcription factor gene family in peanut: Genome-wide characterization and expression analysis under abiotic stress
- Author
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Yifei Mou, Cuiling Yuan, Quanxi Sun, Caixia Yan, Xiaobo Zhao, Juan Wang, Qi Wang, Shihua Shan, and Chunjuan Li
- Subjects
MADS-box transcription factor ,phylogenetic analysis ,expression patterns ,abiotic stress ,peanut ,Plant culture ,SB1-1110 - Abstract
Peanut (Arachis hypogaea) is one of the most important economic crops around the world, especially since it provides vegetable oil and high-quality protein for humans. Proteins encoded by MADS-box transcription factors are widely involved in regulating plant growth and development as well as responses to abiotic stresses. However, the MIKC-type MADS-box TFs in peanut remains currently unclear. Hence, in this study, 166 MIKC-type MADS-box genes were identified in both cultivated and wild-type peanut genomes, which were divided into 12 subfamilies. We found a variety of development-, hormone-, and stress-related cis-acting elements in the promoter region of peanut MIKC-type MADS-box genes. The chromosomal distribution of peanut MADS-box genes was not random, and gene duplication contributed to the expansion of the MADS-box gene family. The interaction network of the peanut AhMADS proteins was established. Expression pattern analysis showed that AhMADS genes were specifically expressed in tissues and under abiotic stresses. It was further confirmed via the qRT-PCR technique that five selected AhMADS genes could be induced by abiotic and hormone treatments and presented different expressive profiles under various stresses. Taken together, these findings provide valuable information for the exploration of candidate genes in molecular breeding and further study of AhMADS gene functions.
- Published
- 2022
- Full Text
- View/download PDF
38. Genomic analysis of the immune gene repertoire of amphioxus reveals extraordinary innate complexity and diversity
- Author
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Shengfeng Huang, Shaochun Yuan, Lei Guo, Jun Li, Tao Wu, Manyi Yang, Huiqing Huang, Meiling Dong, Cuiling Yu, Shangwu Chen, and Anlong Xu
- Subjects
Sea urchins -- Genetic aspects ,Sea urchins -- Research ,Biological diversity -- Research ,Health - Abstract
The first comprehensive genomic survey of the immune gene repertoire of the amphioxus Branchiostoma floridae, the purple sea urchin is reported. The survey observations revealed that the amphioxus, a species without vertebrate-type adaptive immunity holds extraordinary innate complexity and diversity.
- Published
- 2008
39. Electrical property and phase transition of CdSe under high pressure
- Author
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Bingguo Liu, Guangtian Zou, Cuiling Yu, Xiaowei Huang, Hongwu Liu, Chunxiao Gao, Ming Li, Dongmei Zhang, Aimin Hao, Chunyuan He, and Yanzhang Ma
- Subjects
Phase transition ,Condensed matter physics ,Band gap ,Chemistry ,Drop (liquid) ,Mineralogy ,General Chemistry ,Electron ,Condensed Matter Physics ,Diamond anvil cell ,Electrical resistivity and conductivity ,General Materials Science ,Temperature coefficient ,Order of magnitude - Abstract
In situ electrical resistivity measurement of CdSe was performed under high pressure and moderate temperature using a diamond anvil cell equipped with a microcircuit. With the pressure increasing, a sharp drop in resistivity of over two orders of magnitude was observed at about 2.6 GPa, it was caused by the transition to the rock-salt CdSe. After that, the resistivity decreased linearly with pressure. However, in different pressure range, the decreasing degree was obviously different. This attributed to the different electron structures. By fitting to the curve of pressure dependence of resistivity in different pressure range, the relationship of the band gap to pressure was given and the metallization pressure was speculated to be in the range of 70–100 GPa. The temperature dependence of resistivity showed that in the experimental temperature and pressure range the resistivity had a positive temperature coefficient.
- Published
- 2008
40. Genome-wide analyses of amphioxus microRNAs reveal an immune regulation via miR-92d targeting C3
- Author
-
Lei Guo, Shengfeng Huang, Cuiling Yu, Wei Zhu, Ruimin Zhu, Tingting Zheng, Anlong Xu, Xingsheng Cai, Shangwu Chen, Zhenghua Ren, Qingyu Yan, Yingcai Yu, and Rirong Yang
- Subjects
Immunology ,Chordate ,Computational biology ,Adaptive Immunity ,Bioinformatics ,digestive system ,Genome ,Evolution, Molecular ,Immune system ,Immunity ,Chordata, Nonvertebrate ,microRNA ,Immunology and Allergy ,Animals ,Humans ,Messenger RNA ,biology ,HEK 293 cells ,Complement C3 ,Staphylococcal Infections ,biology.organism_classification ,Complement system ,Disease Models, Animal ,MicroRNAs ,HEK293 Cells ,Vibrio Infections ,Gene Targeting ,Genome-Wide Association Study - Abstract
Recently, amphioxus has served as a model for studying the origin and evolution of vertebrate immunity. However, little is known about how microRNAs (miRNAs) are involved in the immune defense in amphioxus. In this article, we present a systematic study of amphioxus miRNAs in the acute-phase response to bacterial infection; miR-92d was found to regulate the complement pathway in this basal chordate. We identified all 155 possible miRNAs present in the amphioxus Branchiostoma belcheri genome by bioinformatics analyses, including 57 newly identified miRNAs (called bbe-miRNAs), and characterized the miRNA expression pattern. Four miRNAs (bbe-miR-7, bbe-miR-4868a, bbe-miR-2065, and bbe-miR-34b) were upregulated and bbe-miR-92d was downregulated under the challenge of both Vibrio anguillarum and Staphylococcus aureus bacteria. We further predicted miRNA targets and identified mRNA targets of immune-related miRNA using the hybrid PCR method. We propose that miR-92d regulates the complement pathway through targeting C3 for controlling the acute immune response to bacterial infections. This study provides evidence for the complex immune regulation of miRNAs in the acute-phase response in basal chordates.
- Published
- 2013
41. An Improved Squeaky Wheel Optimization Approach to the Airport Gates Assignment Problem
- Author
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Xue-Yan Song and Cuiling Yu
- Subjects
Mathematical optimization ,Degree (graph theory) ,Computer science ,ComputerApplications_COMPUTERSINOTHERSYSTEMS ,Assignment problem ,Tabu search - Abstract
The optimization of the airport gates assignment problem aims to improve the efficiency of gates allocation as well as the satisfaction degree of passengers. In this paper, an SWO algorithm combined with Tabu Search strategy was proposed to optimize the allocation of airport gates. Experimental results show that the improved SWO approach is more efficient than the conventional SWO approach.
- Published
- 2013
42. Research on evolution mechanism of enterprise IT outsourcing relationship
- Author
-
Xinhua Bi, Cuiling Yu, and Weihua Duan
- Subjects
Government ,ComputingMilieux_THECOMPUTINGPROFESSION ,business.industry ,Computer science ,Information technology ,Competitor analysis ,Knowledge process outsourcing ,Technology management ,Outsourcing ,Competition (economics) ,ComputingMilieux_MANAGEMENTOFCOMPUTINGANDINFORMATIONSYSTEMS ,InformationSystems_GENERAL ,Project management ,business ,Industrial organization - Abstract
As competition intensifies, more and more enterprises outsource their IT. It is very important to enterprises that how to make IT outsourcing success. Outsourcing Relationship is one of the key issues of IT outsourcing. This paper firstly introduces the importance of IT outsourcing relationship; secondly, it analyses evolution dynamic of IT outsourcing relationship: external power including government, competitors, technology, suppliers and internal power including enterprise's management ability, top management and outsourcing project; thirdly, it analyses evolution path of IT outsourcing relationship: support, alignment, reliance and alliance; lastly, it forms evolution mechanism model to explain evolution mechanism of enterprise IT outsourcing relationship.
- Published
- 2010
43. Research on formation mechanism of enterprise IT outsourcing relationship
- Author
-
Xinhua Bi, Weihua Duan, and Cuiling Yu
- Subjects
InformationSystems_GENERAL ,Government ,Process management ,Core business ,business.industry ,Information technology ,Competitor analysis ,Project management ,business ,Knowledge process outsourcing ,Technology management ,Outsourcing - Abstract
In order to survive more and more enterprises have to put more attention to its core business and outsource its complex information technology. For many enterprises, what matters is no longer whether IT outsourcing should be practiced but how it should be well managed. Outsourcing Relationship is one of the key issues of IT outsourcing. This paper firstly reviews the literature about IT outsourcing relationships; secondly, it analyses dynamic mechanism of IT outsourcing relationship formation: external power including government, competitors, technology, suppliers' industrial level and internal power including enterprise's management ability, top management and outsourcing project; lastly, it forms one model and one analysis process to explain formation mechanism of enterprise IT outsourcing relationship.
- Published
- 2010
44. Analysis of industry differences in information systems growth of enterprises in northeast old industrial base
- Author
-
Xiaoyun Qi, Wan Su, Cuiling Yu, and Xinhua Bi
- Subjects
Process (engineering) ,Business data processing ,Mode (statistics) ,Information system ,Business ,Information industry ,Informatization ,Marketing ,Field (geography) ,Industrial organization - Abstract
It collected and processed the history data of their information system construction through an investigation of 222 enterprises in northeast old industrial base, explored the industry differences in growth process of information systems of enterprises. By analysis, we found that there were differences in the time of starting to implement informatization and starting to build a LAN, development mode and application field of information system of different industries. At last we concluded the reasons caused the differences, proposed several useful countermeasures and suggestions.
- Published
- 2009
45. Empirical Study on the Dynamic Mechanism of Enterprise Information System Growth
- Author
-
Taibo Chen, Xiaoyun Qi, Xinhua Bi, and Cuiling Yu
- Subjects
Knowledge management ,Empirical research ,Process management ,Construction industry ,business.industry ,Information system ,Information technology ,Enterprise information system ,business ,Mechanism (sociology) - Published
- 2009
46. Absorptive Capacity: Enhancing the Absorption of Information Technology
- Author
-
Taibo Chen, Xiaoyun Qi, Xinhua Bi, and Cuiling Yu
- Subjects
Commerce ,Absorptive capacity ,business.industry ,Information technology ,Context (language use) ,Research questions ,business ,Absorption (electromagnetic radiation) ,Industrial organization - Abstract
This paper studies how to enhance information technology (IT) absorption from the perspective of absorptive capacity. The research questions for this study are: What is the impact of absorptive capacity on a firm's IT absorption, and what would cause some firms to have a higher IT absorptive capacity than other firms? We examine the role of absorptive capacity in IT absorption, and identify five factors influencing IT absorptive capacity. The method of stochastic frontier estimation is used to estimate IT absorptive capacity and then explain the relationship between the five factors and IT absorptive capacity. Keywords-information technology(IT); IT absorption; IT absorptive capacity
- Published
- 2009
47. Dual nature of the adaptive immune system in lampreys
- Author
-
Peng Guo, Jianxu Li, Brantley R. Herrin, Max D. Cooper, Cuiling Yu, Masayuki Hirano, and Andrea Sadlonova
- Subjects
Fish Proteins ,T cell ,animal diseases ,Amino Acid Motifs ,chemical and pharmacologic phenomena ,Article ,Cytosine Deaminase ,Immune system ,Variable lymphocyte receptor ,Antigen ,medicine ,Animals ,Lymphocytes ,Antigens ,Phytohemagglutinins ,Receptors, Immunologic ,Macrophage Migration-Inhibitory Factors ,Alleles ,Genetics ,Multidisciplinary ,biology ,Gene Expression Profiling ,Cytosine deaminase ,Interleukin-17 ,Lampreys ,T lymphocyte ,biochemical phenomena, metabolism, and nutrition ,Acquired immune system ,Biological Evolution ,Cell biology ,medicine.anatomical_structure ,biology.protein ,bacteria ,Antibody ,Mitogens ,human activities - Abstract
Jawless vertebrates use variable lymphocyte receptors (VLR) comprised of leucine-rich-repeat (LRR) segments as counterparts of the immunoglobulin-based receptors that jawed vertebrates use for antigen recognition. Highly diverse VLR genes are somatically assembled by the insertion of variable LRR sequences into incomplete germline VLRA and VLRB genes. Here we show that in sea lampreys (Petromyzon marinus) VLRA and VLRB anticipatory receptors are expressed by separate lymphocyte populations by monoallelic VLRA or VLRB assembly, together with expression of cytosine deaminase 1 (CDA1) or 2 (CDA2), respectively. Distinctive gene expression profiles for VLRA(+) and VLRB(+) lymphocytes resemble those of mammalian T and B cells. Although both the VLRA and the VLRB cells proliferate in response to antigenic stimulation, only the VLRB lymphocytes bind native antigens and differentiate into VLR antibody-secreting cells. Conversely, VLRA lymphocytes respond preferentially to a classical T-cell mitogen and upregulate the expression of the pro-inflammatory cytokine genes interleukin-17 (IL-17) and macrophage migration inhibitory factor (MIF). The finding of T-like and B-like lymphocytes in lampreys offers new insight into the evolution of adaptive immunity.
- Published
- 2009
48. Survival Analysis on Information Technology Adoption of Chinese Enterprises
- Author
-
Cuiling Yu and Xinhua Bi
- Subjects
Knowledge management ,business.industry ,Information system ,Office automation ,Information technology ,Regression analysis ,Dimension (data warehouse) ,business ,It adoption ,Enterprise resource planning ,Survival analysis - Abstract
This paper takes three typical information systems (financial system, office automatic system, and enterprise resource planning system) as examples to study information technology (IT) adoption of Chinese enterprises by means of survival analysis. The dimension of time and dynamic characteristic of IT adoption are highly emphasized in this paper. The method of Kaplan-Meier analysis is used to compare the difference of survival time of IT adoption for heterogeneous enterprises, and the resulting survival functions reflect the adoption proportion at a particular time. Then Cox regression model is used to examine the impact of enterprise characteristics on IT adoption. The results demonstrate that their impact varies with the target technology. Further, this study indicates the direction and significance of the impact of enterprise characteristics on the three technologies respectively.
- Published
- 2008
49. A Research on Synergic and Spiral-Propulsion Mechanism of Enterprise Information Systems Growth
- Author
-
Cuiling Yu and Xinhua Bi
- Subjects
Enterprise systems engineering ,Knowledge management ,Enterprise life cycle ,business.industry ,Enterprise integration ,Integrated enterprise modeling ,Enterprise architecture ,Business ,Enterprise information system ,Enterprise modelling ,Industrial organization ,Enterprise software - Abstract
The mechanism of information system (IS) growth is an important part of IS growth theory and has attracted much attention of scholars and entrepreneurs. As validated by many studies, the process of IS growth is influenced by various factors within the enterprise and from external environment. The external factors are mainly governmental propulsion, competitive pressure, linkage demand of cooperative enterprises and IT suppliers’ propulsion. The internal factors are top management support, middle management support, IT department propulsion and other organizational personnel’s cooperation and participation. The synergic effect of internal and external factors promotes the development of IS construction and the growth of enterprise information systems. This paper pays more attention to the microcosmic mechanism of IS growth and proposes that IT absorption is a direct impetus to IS growth. It analyzes synergic effect of the subjects and elements of IT absorption. Furthermore, a mathematic model is used to describe the spiral-propulsion mode of IS growth. Viewed from a holistic perspective, IS growth presents itself as a dynamic and spiral process.
- Published
- 2007
50. Growth of dumbbell-like ZnO microcrystals under mild conditions and their photoluminescence properties
- Author
-
Guangtian Zou, Guorui Wang, Hongdong Li, Yichun Liu, Qingjiang Yu, Wuyou Fu, Ronghui Wei, Lianxia Chang, Hongyang Zhu, Minghui Li, Changlu Shao, Haibin Yang, Jing Xu, and Cuiling Yu
- Subjects
Inorganic Chemistry ,Crystallography ,Photoluminescence ,Chemical engineering ,Hexagonal crystal system ,Scanning electron microscope ,Chemistry ,Dumbbell like ,Physical and Theoretical Chemistry ,Spectroscopy ,Characterization (materials science) - Abstract
Large-scale uniform dumbbell-like ZnO microcrystals were successfully synthesized via a facile solution method under mild conditions. The as-prepared dumbbells, with lengths of 3.5-5.4 microm and diameters of 1.3-1.8 microm, possess a single-crystal hexagonal structure and grow along the [0001] direction. The influence of the reactant concentration on the size and shapes of the ZnO samples had been studied, and the results revealed that the reactant concentration plays a crucial role in determining final morphologies of the samples. Moreover, the evolution process of the dumbbell-like ZnO microcrystals was viewed by field-emission scanning electron microscopy (FE-SEM) characterization, and a possible formation mechanism was proposed. In addition, optical properties of the ZnO samples prepared at different reaction times were also investigated by photoluminescence (PL) spectroscopy. The room-temperature PL spectrum of the dumbbell-like ZnO microcrystals shows a strong UV emission peak. The UV emission is further identified to originate from the radiative free-exciton recombination by the temperature-dependent PL.
- Published
- 2007
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