1. Superoxide dismutase and oxidative stress in Dahl salt-sensitive and -resistant rats
- Author
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Meng, Shumei, Roberts, L. Jackson, II, Cason, Garrick W., Curry, Travis S., Manning, R. Davis, Jr., Mosconi, G., Carnevali, O., and Habibi, H.R.
- Subjects
Oxidation, Physiological -- Analysis ,Superoxide dismutase -- Physiological aspects ,Kidneys -- Physiological aspects ,Renal hypertension -- Physiological aspects ,Biological sciences - Abstract
The roles of oxidative stress and renal superoxide dismutase (SOD) levels and their association with renal damage were studied in Dahl salt-sensitive (S) and salt-resistant (R)/Rapp strain rats during changes in Na intake. After 3 wk of a high (8%)-Na diet in S rats, renal medullary Cu/Zn SOD was 56% lower and Mn SOD was 81% lower than in R high Na-fed rats. After 1, 2, and 3 wk of high Na, urinary excretion of [F.sub.2]-isoprostanes, an index of oxidative stress, was significantly greater in S rats compared with R rats. Plasma [F.sub.2]-isoprostane concentration increased in the 2-wk S high Na-fed group. After 3 wk, renal cortical and medullary superoxide production was significantly increased in Dahl S rats on high Na intake, and urinary protein excretion, an index of renal damage, was 273 [+ or -] 32 mg/d in S high Na-fed rats and 35 [+ or -] 4 mg/d in R high Na-fed rats (P < 0.05). In conclusion, salt-sensitive hypertension in the S rat is accompanied by marked decreases in renal medullary SOD and greater renal oxidative stress and renal damage than in R rats. urinary protein excretion; isoprostane; dietary sodium; renal damage
- Published
- 2002