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5. Compressive forces stabilize microtubules in living cells

Author

Sub Cell Biology, Cell Biology, Neurobiology and Biophysics, Li, Yuhui, Kučera, Ondřej, Cuvelier, Damien, Rutkowski, David M., Deygas, Mathieu, Rai, Dipti, Pavlovič, Tonja, Vicente, Filipe Nunes, Piel, Matthieu, Giannone, Grégory, Vavylonis, Dimitrios, Akhmanova, Anna, Blanchoin, Laurent, Théry, Manuel, Sub Cell Biology, Cell Biology, Neurobiology and Biophysics, Li, Yuhui, Kučera, Ondřej, Cuvelier, Damien, Rutkowski, David M., Deygas, Mathieu, Rai, Dipti, Pavlovič, Tonja, Vicente, Filipe Nunes, Piel, Matthieu, Giannone, Grégory, Vavylonis, Dimitrios, Akhmanova, Anna, Blanchoin, Laurent, and Théry, Manuel

Published
2023

6. How cells flow in the spreading of cellular aggregates

Author

Beaune, Grègory, Stirbat, Tomita Vasilica, Khalifat, Nada, Cochet-Escartin, Olivier, Garcia, Simon, Gurchenkov, Vasily Valèrïèvitch, Murrell, Michael P., Dufour, Sylvie, Cuvelier, Damien, and Brochard-Wyart, Françoise

Published
2014

12. A mechano-osmotic feedback couples cell volume to the rate of cell deformation

Author

Venkova, Larisa, primary, Vishen, Amit Singh, additional, Lembo, Sergio, additional, Srivastava, Nishit, additional, Duchamp, Baptiste, additional, Ruppel, Artur, additional, Williart, Alice, additional, Vassilopoulos, Stéphane, additional, Deslys, Alexandre, additional, Garcia Arcos, Juan Manuel, additional, Diz-Muñoz, Alba, additional, Balland, Martial, additional, Joanny, Jean-François, additional, Cuvelier, Damien, additional, Sens, Pierre, additional, and Piel, Matthieu, additional

Published
2022
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13. Author response: A mechano-osmotic feedback couples cell volume to the rate of cell deformation

Author

Venkova, Larisa, primary, Vishen, Amit Singh, additional, Lembo, Sergio, additional, Srivastava, Nishit, additional, Duchamp, Baptiste, additional, Ruppel, Artur, additional, Williart, Alice, additional, Vassilopoulos, Stéphane, additional, Deslys, Alexandre, additional, Garcia Arcos, Juan Manuel, additional, Diz-Muñoz, Alba, additional, Balland, Martial, additional, Joanny, Jean-François, additional, Cuvelier, Damien, additional, Sens, Pierre, additional, and Piel, Matthieu, additional

Published
2022
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14. Compressive forces stabilise microtubules in living cells

Author

Li, Yuhui, primary, Kučera, Ondřej, additional, Cuvelier, Damien, additional, Rutkowski, David M., additional, Deygas, Mathieu, additional, Rai, Dipti, additional, Pavlovič, Tonja, additional, Vicente, Filipe Nunes, additional, Piel, Matthieu, additional, Giannone, Gregory, additional, Vavylonis, Dimitrios, additional, Akhmanova, Anna, additional, Blanchoin, Laurent, additional, and Théry, Manuel, additional

Published
2022
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17. Phenotyping Polarization Dynamics Of Immune Cells Using A Lipid Droplet - Cell Pairing Microfluidic Platform

Author

Pinon, Léa, primary, Ruyssen, Nicolas, additional, Pineau, Judith, additional, Mesdjian, Olivier, additional, Cuvelier, Damien, additional, Chipont, Anna, additional, Allena, Rachele, additional, Guerin, Coralie L., additional, Asnacios, Sophie, additional, Asnacios, Atef, additional, Pierobon, Paolo, additional, and Fattaccioli, Jacques, additional

Published
2021
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19. Hematopoietic progenitors polarize in contact with bone marrow stromal cells in response to SDF1

Author

Bessy, Thomas, primary, Candelas, Adrian, additional, Souquet, Benoit, additional, Saadallah, Khansa, additional, Schaeffer, Alexandre, additional, Vianay, Benoit, additional, Cuvelier, Damien, additional, Gobaa, Samy, additional, Nakid-Cordero, Cecilia, additional, Lion, Julien, additional, Bories, Jean-Christophe, additional, Mooney, Nuala, additional, Jaffredo, Thierry, additional, Larghero, Jerome, additional, Blanchoin, Laurent, additional, Faivre, Lionel, additional, Brunet, Stephane, additional, and Théry, Manuel, additional

Published
2021
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21. A mechano-osmotic feedback couples cell volume to the rate of cell deformation

Author

Venkova, Larisa, primary, Vishen, Amit Singh, additional, Lembo, Sergio, additional, Srivastava, Nishit, additional, Duchamp, Baptiste, additional, Ruppel, Artur, additional, Vassilopoulos, Stéphane, additional, Deslys, Alexandre, additional, Arcos, Juan Manuel Garcia, additional, Diz-Muñoz, Alba, additional, Balland, Martial, additional, Joanny, Jean-François, additional, Cuvelier, Damien, additional, Sens, Pierre, additional, and Piel, Matthieu, additional

Published
2021
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27. Innate Immune Signals Induce Anterograde Endosome Transport Promoting MHC Class I Cross-Presentation

Author

Beaune, Gregory, Blanch-Mercader, Carles, Douezan, Stephane, Dumond, Julien, Gonzalez-Rodriguez, David, Cuvelier, Damien, Ondarçuhu, Thierry, Sens, Pierre, Dufour, Sylvie, Murrell, Michael, Charles-Orszag, Arthur, Tsai, Feng-Ching, Bonazzi, Daria, Manriquez, Valeria, Sachse, Martin, Mallet, Adeline, Salles, Audrey, Melican, Keira, Staneva, Ralitza, Bertin, Aurélie, Millien, Corinne, Goussard, Sylvie, Lafaye, Pierre, Shorte, Spencer, Piel, Matthieu, Krijnse-Locker, Jacomine, Brochard-Wyart, Françoise, Bassereau, Patricia, Duménil, Guillaume, Weimershaus, Mirjana, Mauvais, Francois-Xavier, Saveanu, Loredana, Adiko, Cézaire, Babdor, Joel, Abramova, Anastasia, Montealegre, Sebastian, Lawand, Myriam, Evnouchidou, Irini, Huber, Katharina Julia, Chadt, Alexandra, Zwick, Markus, Vargas, Pablo, Dussiot, Michaël, Lennon-Dumenil, Ana Maria, Brocker, Thomas, Al-Hasani, Hadi, Van Endert, Peter, National Institute for Materials Science (NIMS), Physico-Chimie-Curie (PCC), Institut Curie-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS), Centre National de la Recherche Scientifique (CNRS)-Institut Curie-Université Pierre et Marie Curie - Paris 6 (UPMC), Centre d'élaboration de matériaux et d'études structurales (CEMES), Institut National des Sciences Appliquées - Toulouse (INSA Toulouse), Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Institut de Chimie de Toulouse (ICT-FR 2599), Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut de Recherche pour le Développement (IRD)-Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Centre National de la Recherche Scientifique (CNRS)-Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS), Biologie des Organismes et Ecosystèmes Aquatiques (BOREA), Université de Caen Normandie (UNICAEN), Normandie Université (NU)-Normandie Université (NU)-Muséum national d'Histoire naturelle (MNHN)-Institut de Recherche pour le Développement (IRD)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Université des Antilles (UA), Paris-Centre de Recherche Cardiovasculaire (PARCC - UMR-S U970), Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Hôpital Européen Georges Pompidou [APHP] (HEGP), Assistance publique - Hôpitaux de Paris (AP-HP) (APHP)-Hôpitaux Universitaires Paris Ouest - Hôpitaux Universitaires Île de France Ouest (HUPO)-Assistance publique - Hôpitaux de Paris (AP-HP) (APHP)-Hôpitaux Universitaires Paris Ouest - Hôpitaux Universitaires Île de France Ouest (HUPO), Institut Jacques Monod (IJM (UMR_7592)), Centre National de la Recherche Scientifique (CNRS)-Université Paris Diderot - Paris 7 (UPD7), Pathogénèse des Infections vasculaires, Institut Pasteur [Paris]-Institut National de la Santé et de la Recherche Médicale (INSERM), Microscopie ultrastructurale (plate-forme), Institut Pasteur [Paris], Imagopole (CITECH), Centre d'Immunologie de Marseille - Luminy (CIML), Aix Marseille Université (AMU)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Department of Neuroscience, Karolinska Institutet [Stockholm], Institut Curie, SAFT [Bordeaux], Société des accumulateurs fixes et de traction (SAFT), Ingénierie des Anticorps (plate-forme) - Antibody Engineering (Platform), Institut Pasteur [Paris]-Centre National de la Recherche Scientifique (CNRS), Compartimentation et dynamique cellulaires (CDC), Microscopie ultrastructurale - Ultrapole (CITECH), Diabète de Type 1 : mécanismes et traitements immunologiques, Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM), Institut Necker Enfants-Malades (INEM - UM 111 (UMR 8253 / U1151)), Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Centre de recherche sur l'Inflammation (CRI (UMR_S_1149 / ERL_8252 / U1149)), Université Paris Diderot - Paris 7 (UPD7)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Centre de Recherche des Cordeliers (CRC), Université Paris Diderot - Paris 7 (UPD7)-École pratique des hautes études (EPHE)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM), Immunité et cancer, Institut Curie-Institut National de la Santé et de la Recherche Médicale (INSERM), Hémostase, bio-ingénierie et remodelage cardiovasculaires (LBPC), Université Paris 13 (UP13)-Université Paris Diderot - Paris 7 (UPD7)-Institut Galilée-Université Sorbonne Paris Cité (USPC)-Institut National de la Santé et de la Recherche Médicale (INSERM), Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Université Paris Diderot - Paris 7 (UPD7), Université Paris Diderot - Paris 7 (UPD7)-École pratique des hautes études (EPHE), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM), Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut Curie [Paris], Université Paris Diderot - Paris 7 (UPD7)-Université Paris 13 (UP13)-Université Sorbonne Paris Cité (USPC)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut Galilée, and Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Paris Diderot - Paris 7 (UPD7)-Centre National de la Recherche Scientifique (CNRS)

Subjects
0301 basic medicine, Male, Endosome, Antigen presentation, Fc receptor, Kinesins, Endosomes, Receptors, Fc, [SDV.BC.BC]Life Sciences [q-bio]/Cellular Biology/Subcellular Processes [q-bio.SC], Microtubules, General Biochemistry, Genetics and Molecular Biology, 03 medical and health sciences, Mice, Cross-Priming, Phagosomes, Animals, lcsh:QH301-705.5, Cells, Cultured, ComputingMilieux_MISCELLANEOUS, Phagosome, Innate immune system, biology, Chemistry, Histocompatibility Antigens Class I, Cross-presentation, Dendritic cell, Immunity, Innate, Cell biology, Toll-Like Receptor 4, Protein Transport, 030104 developmental biology, lcsh:Biology (General), rab GTP-Binding Proteins, biology.protein, Kinesin, Female
Abstract

Summary: Both cross-presentation of antigens by dendritic cells, a key pathway triggering T cell immunity and immune tolerance, and survival of several pathogens residing in intracellular vacuoles are intimately linked to delayed maturation of vesicles containing internalized antigens and microbes. However, how early endosome or phagosome identity is maintained is incompletely understood. We show that Toll-like receptor 4 (TLR4) and Fc receptor ligation induces interaction of the GTPase Rab14 with the kinesin KIF16b mediating plus-end-directed microtubule transport of endosomes. As a result, Rab14 recruitment to phagosomes delays their maturation and killing of an internalized pathogen. Enhancing anterograde transport by overexpressing Rab14, promoting the GTP-bound Rab14 state, or inhibiting retrograde transport upregulates cross-presentation. Conversely, reducing Rab14 expression, destabilizing Rab14 endosomes, and inhibiting anterograde microtubule transport by Kif16b knockdown compromise cross-presentation. Therefore, regulation of early endosome trafficking by innate immune signals is a critical parameter in cross-presentation by dendritic cells. : Weimershaus et al. identify a molecular complex that controls the intracellular trafficking along microtubules of antigens internalized by dendritic cells. They show that this trafficking is regulated by innate immune signals and regulates presentation of internalized antigens to T lymphocytes. Keywords: antigen presentation, cross-presentation, dendritic cell, MHC class I, endosome, small GTPase, kinesin, Rab14

Published
2018
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29. Mechanotransduction at the Nuclear Envelope

Author

Déjardin, Théophile, primary, Davidson, Patricia, additional, Seiler, Cynthia, additional, Girard, Philippe, additional, Cuvelier, Damien, additional, Sykes, Cécile, additional, Gomes, Edgar, additional, Bruno, Cadot, additional, and Borghi, Nicolas, additional

Published
2017
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30. Applying mechanical forces on Drosophilatissues in vivousing the StretchCo, a 3D-printable device

Author

Gracia, Mélanie, Lefèvre, Bénédicte M., Güell Alonso, Raquel, Cachoux, Victoire, Balakireva, Maria, Guirao, Boris, Cuvelier, Damien, Bardin, Allison J., and Bellaïche, Yohanns

Abstract

Applying mechanical forces to tissues helps to understand morphogenesis and homeostasis. Additionally, recording the dynamics of living tissues under mechanical constraints is needed to explore tissue biomechanics. Here, we present a protocol to 3D-print a StretchCo device and use it to apply uniaxial mechanical stress on the Drosophilapupal dorsal thorax epithelium. We describe steps for 3D printing, polydimethylsiloxane (PDMS) strip cutting, and glue preparation. We detail procedures for PDMS strip mounting, tissue compaction, and live imaging upon force application. For additional details on the use and execution of this protocol, please refer to Cachoux et al. (2023)1from which the StretchCo machine has been derived.

Published
2024
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31. Adhésion cellulaire et tubes de membrane : Quelques aspects dynamiques, mécaniques et rhéologiques

Author

Cuvelier, Damien, Physico-Chimie-Curie (PCC), Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut Curie [Paris]-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), Université Pierre et Marie Curie - Paris VI, Bassereau Patricia(patricia.bassereau@curie.fr), and Centre National de la Recherche Scientifique (CNRS)-Institut Curie [Paris]-Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut de Chimie du CNRS (INC)

Subjects
surface functionnalization, membrane tubes, [PHYS.PHYS.PHYS-BIO-PH]Physics [physics]/Physics [physics]/Biological Physics [physics.bio-ph], soft lythography, cell adhesion, red blood cell, tube de membrane, fonctionnalisation de surface, optical tweezer, adhesion, lythographie molle, micropipette, pince optique, globule rouge, membrane
Abstract

In the "adhesion" section, we have shown that the dynamics of vesicleadhesion induced by specific stickers was either governed by the diffusion of ligandsin the membrane or by the reaction time between immobilized receptors and ligands,depending on the chemical preparation of the decorated substrate. In contrast, theearly stages of living cell adhesion seem to be controlled by the viscous dissipationinside the cell.In the "tubes" section, we have studied the formation and the elongation ofmembrane tethers. First, by pulling long tubes from firmly adhered vesicles, wedemonstrated that the resulting unusual force-extension trace was the signature ofan elastic stretching of the lipid membrane. Second, by analyzing both experimentallyand theoretically the interaction and coalescence of two tethers, we proposed a novelmethod to determine the bending rigidity of phospholipid vesicles. Third, we haverevisited the mechanical and rheological description of tethers extracted from redblood cells and we evidenced a shear-thinning behavior of the membrane during theelongation, which indicates the significant influence of the underlying spectrinnetwork.; Dans la partie "adhésion", nous avons montré que la dynamique d'adhésionde vésicules induite par des ligands spécifiques était gouvernée soit par la diffusionde ligands dans la membrane, soit par le temps de réaction entre le ligand et lerécepteur, dépendant de la préparation chimique des surfaces. Au contraire, lespremières étapes de l'adhésion de cellules semblent être contrôlées par ladissipation visqueuse à l'intérieur de la cellule.Dans la partie "tubes de membrane", nous avons étudié la formation etl'élongation de tubes de membrane. Tout d'abord, en formant des tubes à partir devésicules adhérées, nous avons montré que l'élongation des tubes s'accompagned'un étirement élastique de la membrane. Ensuite, en analysant expérimentalementet théoriquement l'interaction et la coalescence de deux tubes membranaires, nousavons proposé une méthode pour déterminer la rigidité de courbure de vésiculeslipidiques. Enfin, nous avons revisité la description mécanique de tubes extraits deglobules rouges et nous avons mis en évidence un comportement rhéofluidifiant dela membrane durant l'élongation, indiquant l'influence du squelette de spectrine.

Published
2005

36. External forces control mitotic spindle positioning

Author

Fink, Jenny, primary, Carpi, Nicolas, additional, Betz, Timo, additional, Bétard, Angelique, additional, Chebah, Meriem, additional, Azioune, Ammar, additional, Bornens, Michel, additional, Sykes, Cecile, additional, Fetler, Luc, additional, Cuvelier, Damien, additional, and Piel, Matthieu, additional

Published
2011
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45. A Magnetic Pincher for the Dynamic Measurement of the Actin Cortex Thickness in Live Cells.

Author

Vermeil J, Laplaud V, Jawahar A, Bujaa D, Cuvelier D, Heuvingh J, du Roure O, and Piel M

Subjects
Animals, Humans, Actins metabolism, Magnetic Fields, Microspheres, Actin Cytoskeleton metabolism, Actin Cytoskeleton ultrastructure
Abstract

The actin cortex is an essential element of the cytoskeleton allowing cells to control and modify their shape. It is involved in cell division and migration. However, probing precisely the physical properties of the actin cortex has proved to be challenging: it is a thin and dynamic material, and its location in the cell-directly under the plasma membrane-makes it difficult to study with standard light microscopy and cell mechanics techniques. In this chapter, we present a novel protocol to probe dynamically the thickness of the cortex and its fluctuations using superparamagnetic microbeads in a uniform magnetic field. A bead ingested by the cell and another outside the cell attract each other due to dipolar forces. By tracking their position with nanometer precision, one can measure the thickness of the cortex pinched between two beads and monitor its evolution in time. We first present the set of elements necessary to realize this protocol: a magnetic field generator adapted to a specific imaging setup and the aforementioned superparamagnetic microbeads. Then we detail the different steps of a protocol that can be used on diverse cell types, adherent or not., (© 2024. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.)

Published
2024
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