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235 results on '"Cyrklaff, Marek"'

5. Putative prefoldin complex subunit 5 of Plasmodium berghei is crucial for microtubule formation and parasite development in the mosquito.

Author

Beyeler, Raphael, Jordan, Melanie, Dorner, Lilian, He, Buyuan, Cyrklaff, Marek, Roques, Magali, Stanway, Rebecca, Frischknecht, Friedrich, and Heussler, Volker

Subjects
PLASMODIUM, OOCYSTS, PLASMODIUM berghei, MOSQUITOES, PARASITE life cycles, MICROTUBULES, SPOROZOITES
Abstract

Plasmodium sporozoite development in and egress from oocysts in the Anopheles mosquito remains largely enigmatic. In a previously performed high‐throughput knockout screen, the putative subunit 5 of the prefoldin complex (PbPCS5, PBANKA_0920100) was identified as essential for parasite development during mosquito and liver stage development. Here we generated and analyzed a PbPCS5 knockout parasite line during its development in the mosquito. Interestingly, PbPCS5 deletion does not significantly affect oocyst formation but leads to a growth defect resulting in aberrantly shaped sporozoites. Sporozoites produced in the absence of PbPCS5 were thinner, markedly elongated, and did, in most cases, not contain a nucleus. Sporozoites contained fewer subpellicular microtubules, which reached deep into the sporoblast during sporogony where they contacted and indented nuclei. These aberrantly shaped sporozoites did not reach the salivary glands, and we, therefore, conclude that PbPCS5 is essential for sporogony and the life cycle progression of the parasite during its mosquito stage. [ABSTRACT FROM AUTHOR]

Published
2024
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9. Cryo-Electron Tomography of Vaccinia Virus

Author

Cyrklaff, Marek, Risco, Cristina, Fernández, Jose Jesús, Jiménez, Maria Victoria, Estéban, Mariano, Baumeister, Wolfgang, Carrascosa, José L., and Rossmann, Michael G.

Published
2005

15. KAHRP dynamically relocalizes to remodeled actin junctions and associates with knob spirals in Plasmodium falciparum-infected erythrocytes

Author

Sanchez, Cecilia P., Patra, Pintu, Scott Chang, Shih-Ying, Karathanasis, Christos, Hanebutte, Lukas, Kilian, Nicole, Cyrklaff, Marek, Heilemann, Mike, Schwarz, Ulrich S., Kudryashev, Mikhail, and Lanzer, Michael

Subjects
Cancer Research, ddc:570, ddc:540, parasitic diseases, ddc:610
Abstract

The knob-associated histidine-rich protein (KAHRP) plays a pivotal role in the pathophysiology of Plasmodium falciparum malaria by forming membrane protrusions in infected erythrocytes, which anchor parasite-encoded adhesins to the membrane skeleton. The resulting sequestration of parasitized erythrocytes in the microvasculature leads to severe disease. Despite KAHRP being an important virulence factor, its physical location within the membrane skeleton is still debated, as is its function in knob formation. Here, we show by super-resolution microscopy that KAHRP initially associates with various skeletal components, including ankyrin bridges, but eventually colocalizes with remnant actin junctions. We further present a 35 Å map of the spiral scaffold underlying knobs and show that a KAHRP-targeting nanoprobe binds close to the spiral scaffold. Single-molecule localization microscopy detected ~60 KAHRP molecules/knob. We propose a dynamic model of KAHRP organization and a function of KAHRP in attaching other factors to the spiral scaffold.

Published
2022

16. Asynchronous nuclear cycles in multinucleated Plasmodium falciparum facilitate rapid proliferation

Author

Klaus, Severina, primary, Binder, Patrick, additional, Kim, Juyeop, additional, Machado, Marta, additional, Funaya, Charlotta, additional, Schaaf, Violetta, additional, Klaschka, Darius, additional, Kudulyte, Aiste, additional, Cyrklaff, Marek, additional, Laketa, Vibor, additional, Höfer, Thomas, additional, Guizetti, Julien, additional, Becker, Nils B., additional, Frischknecht, Friedrich, additional, Schwarz, Ulrich S., additional, and Ganter, Markus, additional

Published
2022
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17. Transcellular blood–brain barrier disruption in malaria-induced reversible brain edema

Author

Jin, Jessica, primary, Ba, Mame Aida, additional, Wai, Chi Ho, additional, Mohanty, Sanjib, additional, Sahu, Praveen K, additional, Pattnaik, Rajyabardhan, additional, Pirpamer, Lukas, additional, Fischer, Manuel, additional, Heiland, Sabine, additional, Lanzer, Michael, additional, Frischknecht, Friedrich, additional, Mueller, Ann-Kristin, additional, Pfeil, Johannes, additional, Majhi, Megharay, additional, Cyrklaff, Marek, additional, Wassmer, Samuel C, additional, Bendszus, Martin, additional, and Hoffmann, Angelika, additional

Published
2022
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20. Reversible brain edema in experimental cerebral malaria is associated with transcellular blood-brain barrier disruption and delayed microhemorrhages

Author

Jin, Jessica, primary, Ba, Mame Aida, additional, Wai, Chi Ho, additional, Mohanty, Sanjib, additional, Sahu, Praveen K., additional, Pattnaik, Rajyabardham, additional, Pirpamer, Lukas, additional, Fischer, Manuel, additional, Heiland, Sabine, additional, Lanzer, Michael, additional, Frischknecht, Friedrich, additional, Mueller, Ann-Kristin, additional, Pfeil, Johannes, additional, Majhi, Megharay, additional, Cyrklaff, Marek, additional, Wassmer, Samuel C., additional, Bendszus, Martin, additional, and Hoffmann, Angelika, additional

Published
2021
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23. Co-chaperone involvement in knob biogenesis implicates host-derived chaperones in malaria virulence

Author

Diehl, Mathias, primary, Roling, Lena, additional, Rohland, Lukas, additional, Weber, Sebastian, additional, Cyrklaff, Marek, additional, Sanchez, Cecilia P., additional, Beretta, Carlo A., additional, Simon, Caroline S., additional, Guizetti, Julien, additional, Hahn, Julia, additional, Schulz, Norma, additional, Mayer, Matthias P., additional, and Przyborski, Jude M., additional

Published
2021
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24. KAHRP dynamically relocalizes to remodeled actin junctions and associates with knob spirals in Plasmodium falciparum ‐infected erythrocytes

Author

Sanchez, Cecilia P., primary, Patra, Pintu, additional, Chang, Shih‐Ying Scott, additional, Karathanasis, Christos, additional, Hanebutte, Lukas, additional, Kilian, Nicole, additional, Cyrklaff, Marek, additional, Heilemann, Mike, additional, Schwarz, Ulrich S., additional, Kudryashev, Mikhail, additional, and Lanzer, Michael, additional

Published
2021
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27. Author Reply to Peer Reviews of An extended DNA-free intranuclear compartment organizes centrosomal microtubules in Plasmodium falciparum

Author

Guizetti, Julien, primary, Ganter, Markus, additional, Kim, Juyeop, additional, Cyrklaff, Marek, additional, Klaschka, Darius, additional, Penning, Alexander, additional, Machado, Marta, additional, Voß, Yannik, additional, Bauer, Johanna, additional, Funaya, Charlotta, additional, and Simon, Caroline S., additional

Published
2021
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28. Author Reply to Peer Reviews of KAHRP dynamically relocalizes to remodeled actin junctions and associates with knob spirals in P. falciparum-infected erythrocytes

Author

Lanzer, Michael, primary, Kudryashev, Mikhail, additional, Schwarz, Ulrich S., additional, Heilemann, Mike, additional, Cyrklaff, Marek, additional, Kilian, Nicole, additional, Hanebutte, Lukas, additional, Karathanasis, Christos, additional, Chang, Shih-Ying, additional, Patra, Pintu, additional, and Sanchez, Cecilia P., additional

Published
2021
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33. Asynchronous nuclear cycles in multinucleated Plasmodium falciparum enable rapid proliferation

Author

Klaus, Severina, primary, Binder, Patrick, additional, Kim, Juyeop, additional, Machado, Marta, additional, Funaya, Charlotta, additional, Schaaf, Violetta, additional, Klaschka, Darius, additional, Kudulyte, Aiste, additional, Cyrklaff, Marek, additional, Laketa, Vibor, additional, Höfer, Thomas, additional, Guizetti, Julien, additional, Becker, Nils B., additional, Frischknecht, Friedrich, additional, Schwarz, Ulrich S., additional, and Ganter, Markus, additional

Published
2021
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36. Evolutionary conservation of biogenesis of β-barrel membrane proteins

Author

Paschen, Stefan A., Waizenegger, Thomas, Stan, Tincuta, Preuss, Marc, Cyrklaff, Marek, Hell, Kai, Rapaport, Doron, and Neupert, Walter

Subjects
Environmental issues, Science and technology, Zoology and wildlife conservation
Abstract

Author(s): Stefan A. Paschen [1]; Thomas Waizenegger [1]; Tincuta Stan [1]; Marc Preuss [1]; Marek Cyrklaff [2]; Kai Hell [1, 3]; Doron Rapaport [1, 3]; Walter Neupert (corresponding author) [1] [...]

Published
2003
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43. Differential time‐dependent volumetric and surface area changes and delayed induction of new permeation pathways in P. falciparum‐infected hemoglobinopathic erythrocytes

Author

Waldecker, Mailin, Dasanna, Anil K., Lansche, Christine, Linke, Marco, Srismith, Sirikamol, Cyrklaff, Marek, Sanchez, Cecilia P., Schwarz, Ulrich S., and Lanzer, Michael

Subjects
Hemoglobinopathies, Erythrocytes, Time Factors, Cell Membrane, Host-Pathogen Interactions, Plasmodium falciparum, Original Article, Original Articles, Models, Theoretical, Cell Shape, Permeability, Cell Size
Abstract

During intraerythrocytic development, Plasmodium falciparum increases the ion permeability of the erythrocyte plasma membrane to an extent that jeopardizes the osmotic stability of the host cell. A previously formulated numeric model has suggested that the parasite prevents premature rupture of the host cell by consuming hemoglobin (Hb) in excess of its own anabolic needs. Here, we have tested the colloid‐osmotic model on the grounds of time‐resolved experimental measurements on cell surface area and volume. We have further verified whether the colloid‐osmotic model can predict time‐dependent volumetric changes when parasites are grown in erythrocytes containing the hemoglobin variants S or C. A good agreement between model‐predicted and empirical data on both infected erythrocyte and intracellular parasite volume was found for parasitized HbAA and HbAC erythrocytes. However, a delayed induction of the new permeation pathways needed to be taken into consideration for the latter case. For parasitized HbAS erythrocyte, volumes diverged from model predictions, and infected erythrocytes showed excessive vesiculation during the replication cycle. We conclude that the colloid‐osmotic model provides a plausible and experimentally supported explanation of the volume expansion and osmotic stability of P. falciparum‐infected erythrocytes. The contribution of vesiculation to the malaria‐protective function of hemoglobin S is discussed.

Published
2016

45. Oxidative insult can induce malaria-protective trait of sickle and fetal erythrocytes

Author

Cyrklaff, Marek, primary, Srismith, Sirikamol, additional, Nyboer, Britta, additional, Burda, Kvetoslava, additional, Hoffmann, Angelika, additional, Lasitschka, Felix, additional, Adjalley, Sophie, additional, Bisseye, Cyrille, additional, Simpore, Jacques, additional, Mueller, Ann-Kristin, additional, Sanchez, Cecilia P., additional, Frischknecht, Friedrich, additional, and Lanzer, Michael, additional

Published
2016
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47. Actin-mediated plasma membrane plasticity of the intracellular parasite Theileria annulata

Author

Kühni-Boghenbor Katrin, Ma Min, Lemgruber Leandro, Cyrklaff Marek, Frischknecht Friedreich, Gaschen Véronique, Stoffel Michael, and Baumgartner Martin

Subjects
Cytoplasm, Electron Microscope Tomography, fungi, parasitic diseases, Cell Membrane, Cryoelectron Microscopy, Host-Pathogen Interactions, macromolecular substances, Cell Shape, Theileria annulata, Actins
Abstract

Pathogen-host interactions are modulated at multiple levels by both the pathogen and the host cell. Modulation of host cell functions is particularly intriguing in the case of the intracellular Theileria parasite, which resides as a multinucleated schizont free in the cytosol of the host cell. Direct contact between the schizont plasma membrane and the cytoplasm enables the parasite to affect the function of host cell proteins through direct interaction or through the secretion of regulators. Structure and dynamics of the schizont plasma membrane are poorly understood and whether schizont membrane dynamics contribute to parasite propagation is not known. Here we show that the intracellular Theileria schizont can dynamically change its shape by actively extending filamentous membrane protrusions. We found that isolated schizonts bound monomeric tubulin and in vitro polymerized microtubules, and monomeric tubulin polymerized into dense assemblies at the parasite surface. However, we established that isolated Theileria schizonts free of host cell microtubules maintained a lobular morphology and extended filamentous protrusions, demonstrating that host microtubules are dispensable both forthe maintenance of lobular schizont morphology and for the generation of membrane protrusions. These protrusions resemble nanotubes and extend in an actin polymerization-dependent manner; using cryo-electron tomography, we detected thin actin filaments beneath these protrusions, indicating that their extension is driven by schizont actin polymerization. Thus the membrane of the schizont and its underlying actin cytoskeleton possess intrinsic activity for shape control and likely function as a peri-organelle to interact with and manipulate host cell components.

Published
2012

49. Differential time-dependent volumetric and surface area changes and delayed induction of new permeation pathways in P. falciparum-infected hemoglobinopathic erythrocytes.

Author

Waldecker, Mailin, Dasanna, Anil K., Lansche, Christine, Linke, Marco, Srismith, Sirikamol, Cyrklaff, Marek, Sanchez, Cecilia P., Schwarz, Ulrich S., and Lanzer, Michael

Subjects
PROTOZOAN diseases, PLASMODIUM falciparum, SURFACE area, HEMOGLOBINOPATHY, ERYTHROCYTES, CELL membranes
Abstract

During intraerythrocytic development, Plasmodium falciparum increases the ion permeability of the erythrocyte plasma membrane to an extent that jeopardizes the osmotic stability of the host cell. A previously formulated numeric model has suggested that the parasite prevents premature rupture of the host cell by consuming hemoglobin (Hb) in excess of its own anabolic needs. Here, we have tested the colloid-osmotic model on the grounds of time-resolved experimental measurements on cell surface area and volume. We have further verified whether the colloid-osmotic model can predict time-dependent volumetric changes when parasites are grown in erythrocytes containing the hemoglobin variants S or C. A good agreement between model-predicted and empirical data on both infected erythrocyte and intracellular parasite volume was found for parasitized HbAA and HbAC erythrocytes. However, a delayed induction of the new permeation pathways needed to be taken into consideration for the latter case. For parasitized HbAS erythrocyte, volumes diverged from model predictions, and infected erythrocytes showed excessive vesiculation during the replication cycle. We conclude that the colloid-osmotic model provides a plausible and experimentally supported explanation of the volume expansion and osmotic stability of P. falciparum-infected erythrocytes. The contribution of vesiculation to the malaria-protective function of hemoglobin S is discussed. [ABSTRACT FROM AUTHOR]

Published
2017
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