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The nitrogen isotopes (δ15N) in the organic fraction of accretionary hard part structures, such as fish otoliths, may provide life histories of dietary change. We performed controlled experiments to validate the dynamics of the isotopic signal incorporation into biominerals following dietary shifts and also compared whole-otolith and serial sampling approaches for diet reconstruction. Laboratory-reared Atlantic croaker (Micropogonias undulatus) were switched from a high quality, high δ15N diet (δ15N = 10.7‰) to one of two lower quality, lower δ15N diets (δ15N = 5.9 or 3.7‰). Using the oxidation-denitrifier method, including cleaning protocols required for fossil otoliths, we measured both otolith-bound δ15N (δ15Noto) of whole-otolith subsamples (δ15Nwhole) and sequentially micromilled otolith powders (δ15Nmicro) and compared these results to white muscle tissue and liver δ15N obtained through traditional techniques. Both δ15Nwhole and δ15Nmicro recorded the diet switch, although sampling limitations muted the signal in δ15Nmicro, especially in the slowly growing otoliths of the fish on the lowest quality diet, in which otolith accretion was particularly slow. The timescales at which otoliths and tissues approached the new δ15N after the diet switch varied, but the slowest was for muscle and the fastest was for liver. For δ15Nwhole, there were two factors: (1) the turnover time of the N provided to the otolith and (2) that the otolith is accreting and thus integrating over the entire life history of the fish. Using a model to account for the accretionary growth, turnover time for N supplying otolith growth ranged from 12 to 16 days. In both soft tissues and otoliths, the trophic discrimination factor (the δ15N elevation relative to diet) appears to have been lower in the larval fish, prior to the onset of the diet switches. This raises questions about the interpretation of the δ15Noto of otolith cores; however, the core is typically a minute portion of the total otolith. We conclude that (1) δ15Nwhole is useful for tracking diet despite the whole-life integration of the δ15N signal and (2) δ15Nmicro also records diet, although higher resolution tracking will require further optimization of δ15Nmicro sampling.

Chemical analysis of calcified structures continues to flourish, as analytical and technological advances enable researchers to tap into trace elements and isotopes taken up in otoliths and other archival tissues at ever greater resolution. Increasingly, these tracers are applied to refine age estimation and interpretation, and to chronicle responses to environmental stressors, linking these to ecological, physiological, and life-history processes. Here, we review emerging approaches and innovative research directions in otolith chemistry, as well as in the chemistry of other archival tissues, outlining their value for fisheries and ecosystem-based management, turning the spotlight on areas where such biomarkers can support decision making. We summarise recent milestones and the challenges that lie ahead to using otoliths and archival tissues as biomarkers, grouped into seven, rapidly expanding and application-oriented research areas that apply chemical analysis in a variety of contexts, namely: (1) supporting fish age estimation; (2) evaluating environmental stress, ecophysiology and individual performance; (3) confirming seafood provenance; (4) resolving connectivity and movement pathways; (5) characterising food webs and trophic interactions; (6) reconstructing reproductive life histories; and (7) tracing stock enhancement efforts. Emerging research directions that apply hard part chemistry to combat seafood fraud, quantify past food webs, as well as to reconcile growth, movement, thermal, metabolic, stress and reproductive life-histories provide opportunities to examine how harvesting and global change impact fish health and fisheries productivity. Ultimately, improved appreciation of the many practical benefits of archival tissue chemistry to fisheries and ecosystem-based management will support their increased implementation into routine monitoring. Graphical abstract

The combined use of otolith chemistry and tissue isotopes has the potential to reveal movements, habitat associations and food web interactions at a variety of spatial and temporal scales. Here, we used a combination of otolith Ba:Ca life history transects with muscle tissue δ

While it is common knowledge that Red Drum (Sciaenops ocellatus) inhabit oligohaline waters (salinity . Growth consequences of oligohaline exposure and the relationship of oligohaline residency with river discharge were also investigated. Oligohaline exposure varied most during years 2 and 3 of life. During this time, 22% (n = 26/120 individuals) of Red Drum were oligohaline residents (≥ 90% of these years spent in oligohaline salinities), 34% (n = 41) were meso-polyhaline residents (< 10% of years 2 and 3 spent in oligohaline waters), and 44% (n = 53) spent time in both oligohaline and meso-polyhaline salinities. Trends in oligohaline residency match putative Red Drum life history. Oligohaline residents were present during years 1–6 of life; however, oligohaline residency peaked during the second year of life (n = 37, 31%) and by year 7 no oligohaline residents remained. Growth of oligohaline resident Red Drum during years 2–3 of life was lower than non-resident fish. However, long-term growth consequences of oligohaline residency were not apparent. The proportion of oligohaline residents during years 2 or 3 of life was positively related to river discharge. This divergence in salinity residency by juvenile Red Drum demonstrates that life history diversity was present in this population and that oligohaline waters provided important nursery habitat for fish that successfully recruited to the adult population.

Environmental stress can reshape trophic interactions by excluding predators or rendering prey vulnerable, depending on the relative sensitivity of species to the stressor. Classical models of food web responses to stress predict either complete predator exclusion from stressed areas or complete prey vulnerability if predators are stress tolerant. However, if the consumer response to the stress is individually variable, the result may be a distributive stress model (DSM) whereby predators distribute consumption pressure across a range of prey guilds and their trophic niche is expanded. We test these models in one of the largest hypoxic “Dead Zones” in the world, the northern Gulf of Mexico, by combining geochemical tracers of hypoxia exposure and isotope ratios to assess individual‐level trophic responses. Hypoxia‐exposed fish occupied niche widths that were 14.8% and 400% larger than their normoxic counterparts in two different years, consistent with variable displacement from benthic to pelagic food webs. The degree of isotopic displacement depended on the magnitude of hypoxia exposure. These results are consistent with the DSM and highlight the need to account for individually variable sublethal effects when predicting community responses to environmental stress.

A measurement of the double-polarization observable E for the reaction $$\gamma p\rightarrow \pi ^0 p$$ γ p → π 0 p is reported. The data were taken with the CBELSA/TAPS experiment at the ELSA facility in Bonn using the Bonn frozen-spin butanol (C$$_4$$ 4 H$$_9$$ 9 OH) target, which provided longitudinally-polarized protons. Circularly-polarized photons were produced via bremsstrahlung of longitudinally-polarized electrons. The data cover the photon energy range from $$E_\gamma =600$$ E γ = 600 to 2310 MeV and nearly the complete angular range. The results are compared to and have been included in recent partial wave analyses.

Data on the beam asymmetry Σ in the photoproduction of η mesons off protons are reported for tagged photon energies from 1130 to 1790 MeV (mass range from W=1748 MeV to W=2045 MeV). The data cover the full solid angle that allows for a precise moment analysis. For the first time, a strong cusp effect in a polarization observable has been observed that is an effect of a branch-point singularity at the pη^{'} threshold [E_{γ}=1447 MeV (W=1896 MeV)]. The latest BnGa partial wave analysis includes the new beam asymmetry data and yields a strong indication for the N(1895)1/2^{-} nucleon resonance, demonstrating the importance of including all singularities for a correct determination of partial waves and resonance parameters.

Data on the beam asymmetry Σ in the photoproduction of η mesons off protons are reported for tagged photon energies from 1130 to 1790 MeV (mass range from W=1748 MeV to W=2045 MeV). The data cover the full solid angle that allows for a precise moment analysis. For the first time, a strong cusp effect in a polarization observable has been observed that is an effect of a branch-point singularity at the pη^{'} threshold [E_{γ}=1447 MeV (W=1896 MeV)]. The latest BnGa partial wave analysis includes the new beam asymmetry data and yields a strong indication for the N(1895)1/2^{-} nucleon resonance, demonstrating the importance of including all singularities for a correct determination of partial waves and resonance parameters.

Flood and drought events cause significant freshwater inflow fluctuations in estuaries, potentially leading to physiological stress and altered abundances of pathogens such as Vibrio vulnificus and Perkinsus marinus in oysters. To assess the effects of freshwater pulses to oyster reefs in subtropical estuaries in Texas, this study accomplished two goals: 1) reconstructed a reef-specific history of freshwater pulses through shell stable isotope analysis, 2) quantified the abundance of V. vulnificus and P. marinus through culture-dependent and culture-independent microbiology analyses. Oysters from a low-relief and high-relief reef experienced similar fluctuations in shell isotopes, indicating similar ranges of past environmental conditions. V. vulnificus and P. marinus were detected throughout the study but the abundance of these microorganisms was not correlated with environmental parameters or one another. Importantly, the P. marinus infection intensity was always lower at the high-relief reef, which suggests that high-relief reefs may experience lower infection frequencies.

Establishing links between migration patterns and trophic dynamics is paramount to ecological studies investigating the functional role habitats provide to resident and transient species. Natural tags in fishes, such as otolith chemistry and tissue stable isotopes, can help reconstruct previous environmental and dietary histories, although these approaches are rarely combined. A novel multiproxy natural tag approach was developed to estimate immigration patterns of juvenile Atlantic croaker Micropogonias undulatus, across contrasting salinity gradients in three subtropical estuaries of the western Gulf of Mexico. Juvenile young-of-year Atlantic croaker were collected along a latitudinal gradient that included positive, neutral, and negative estuaries, based on physicochemical (temperature, salinity, dissolved element) and isotopic (δ15N and δ13C) parameters. Otolith elemental chronologies of Sr/Ca and Ba/Ca were used to classify migratory types within each estuary, while tissue-specific isotope ratios revealed time since recent (liver~weeks) and longer term (muscle~months) diet shifts. Nitrogen isotopes in both liver and muscle tissues were highly correlated, suggesting tissue equilibrium and estuarine residence of at least 3 months, with geographic δ15N gradients reflecting the magnitude of anthropogenic nutrient enrichment within each estuary. Differences in isotopic equilibrium of muscle-liver δ13C values and variation in marginal edge otolith Sr/Ca and Ba/Ca suggested recent shifts in carbon source and habitat utilization, reflecting individualized movement across seascapes and connectivity of habitat mosaics. The multiproxy approach presented here identified diverse migration patterns and linked feeding and movement on regional (inter-estuary), local (intra-estuary), and individual scales to improve our understanding of habitat function across estuarine gradients.

Assays of elemental and stable-isotope ratios across growth increments of scales have the potential to provide a non-lethal alternative to otolith chemistry for identifying migration and ontogenetic trophic shifts. A central assumption when employing scales as otolith analogues is that any scale from an individual will provide equivalent information about the chemical history of that fish. This assumption was investigated with multiple scales from wild and captive euryhaline Atlantic tarpon Megalops atlanticus from the north-west Gulf of Mexico. Elemental (Sr:Ca) and isotope-ratio (δ13 C and δ15 N) life-history profiles were compared among multiple scales from each fish. All three chemical proxies showed highly consistent patterns among non-regenerated scales, while patterns in regenerated scales diverged, indicating rapid regrowth of interior scale material at the onset of regeneration. Patterns of Sr:Ca and δ13 C covaried, supporting their use as salinity proxies, while δ15 N patterns were consistent with ontogenetic diet shifts. Water samples taken from aquaria holding captive fish were used to calculate partition coefficients for a suite of elements in M. atlanticus scales for future quantification of migratory movements in the region. Together, these results support the assumption that non-regenerated scales from individual M. atlanticus provide equivalent chemical histories, further validating their use as a viable non-lethal alternative to otoliths.

A measurement of the double-polarization observable E for the reaction \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$$\gamma p\rightarrow \pi ^0 p$$\end{document}γp→π0p is reported. The data were taken with the CBELSA/TAPS experiment at the ELSA facility in Bonn using the Bonn frozen-spin butanol (C\documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$$_4$$\end{document}4H\documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$$_9$$\end{document}9OH) target, which provided longitudinally-polarized protons. Circularly-polarized photons were produced via bremsstrahlung of longitudinally-polarized electrons. The data cover the photon energy range from \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$$E_\gamma =600$$\end{document}Eγ=600 to 2310 MeV and nearly the complete angular range. The results are compared to and have been included in recent partial wave analyses.

The $\overline{{\rm{P}}}\mathrm{ANDA}$ (anti-Proton ANnihiliation at DArmstadt) experiment will be one of the four flagship experiments at the new international accelerator complex FAIR (Facility for Antiproton and Ion Research) in Darmstadt, Germany. $\overline{{\rm{P}}}\mathrm{ANDA}$ will address fundamental questions of hadron physics and quantum chromodynamics using high-intensity cooled antiproton beams with momenta between 1.5 and 15 GeV/c and a design luminosity of up to 2 × 1032 cm−2 s−1. Excellent particle identification (PID) is crucial to the success of the $\overline{{\rm{P}}}\mathrm{ANDA}$ physics program. Hadronic PID in the barrel region of the target spectrometer will be performed by a fast and compact Cherenkov counter using the detection of internally reflected Cherenkov light (DIRC) technology. It is designed to cover the polar angle range from 22° to 140° and will provide at least 3 standard deviations (s.d.) π/K separation up to 3.5 GeV/c, matching the expected upper limit of the final state kaon momentum distribution from simulation. This documents describes the technical design and the expected performance of the $\overline{{\rm{P}}}\mathrm{ANDA}$ Barrel DIRC detector. The design is based on the successful BaBar DIRC with several key improvements. The performance and system cost were optimized in detailed detector simulations and validated with full system prototypes using particle beams at GSI and CERN. The final design meets or exceeds the PID goal of clean π/K separation with at least 3 s.d. over the entire phase space of charged kaons in the Barrel DIRC.

A Correction to this paper has been published: 10.1007/s10641-021-01096-6

Bound nuclear systems with two units of strangeness are still poorly known despite their importance for many strong interaction phenomena. Stored antiprotons beams in the GeV range represent an unparalleled factory for various hyperon-antihyperon pairs. Their outstanding large production probability in antiproton collisions will open the floodgates for a series of new studies of systems which contain two or even more units of strangeness at the PANDA experiment at FAIR. For the first time, high resolution gamma-spectroscopy of doubly strange Lambda Lambda-hypernuclei will be performed, thus complementing measurements of ground state decays of Lambda Lambda-hypernuclei at J-PARC or possible decays of particle unstable hypernuclei in heavy ion reactions. High resolution spectroscopy of multistrange Xi(-) -atoms will be feasible and even the production of Omega(-) -atoms will be within reach. The latter might open the door to the vertical bar S vertical bar = 3 world in strangeness nuclear physics, by the study of the hadronic Omega(-) -nucleus interaction. For the first time it will be possible to study the behavior of Xi(+) in nuclear systems under well controlled conditions. (C) 2016 Elsevier B.V. All rights reserved.

Tissue stable isotopes can be used for dietary reconstruction provided that the factors influencing turnover rates and trophic discrimination factors (TDFs) between consumer tissues and diet are known. This experiment quantified δ 13 C and δ 15 N dynamics in muscle and liver tissues in lab-reared juvenile marine fish after a switch from a high quality control diet to medium and low quality diets with decreasing protein and lipid contents. Turnover of δ 15 N in the liver was strongly influenced by metabolism, equilibrating 3 × faster compared to muscle for both diets. Nitrogen TDFs were dependent on diet quality, with values ranging from 3.0–6.5‰ in the muscle and 1.5–3.0‰ in the liver. The effects of mathematical lipid correction on δ 13 C turnover and discrimination were examined by developing novel empirical equations involving C:N ratios and lipid δ 13 C values. Lipid correction affected estimates of isotope turnover in the low quality diet treatment, with lipid-corrected muscle carbon isotopes equilibrating to diet 2 × faster than non-corrected muscle, due to lipid retention increasing turnover estimates for non-corrected values. Conversely, lipid-corrected liver half-lives were 4.4 × slower than non-corrected liver because lipid catabolism increased turnover rates for non-corrected values. A shift in control fish liver carbon TDFs of 2.1 and 1.7‰ for non-corrected and lipid-corrected values, respectively, between the beginning and end of the experiment was attributed to a 32% increase in lipid content. These results demonstrate that metabolic routing of lipid macromolecules strongly influences tissue-specific turnover, and is important to consider when reconstructing trophic dynamics.

The polarization observables T , E , P , H , and G in photoproduction of η mesons off protons are measured for photon energies from threshold to W = 2400 MeV (T), 2280 MeV (E), 1620 MeV ( P , H ), or 1820 MeV (G), covering nearly the full solid angle. The data are compared to predictions from the SAID, MAID, JuBo, and BnGa partial-wave analyses. A refit within the BnGa approach including further data yields precise branching ratios for the Nη decay of nucleon resonances. A Nη-branching ratio of 0.33 ± 0.04 for N ( 1650 ) 1 / 2 − is found, which reduces the large and controversially discussed Nη-branching ratio difference of the two lowest mass J P = 1 / 2 − -resonances significantly.

The production of $\eta^{\prime}$ mesons in coincidence with forward-going protons has been studied in photon-induced reactions on 12C and on a liquid hydrogen (LH2) target for incoming photon energies of 1.3-2.6 GeV at the electron accelerator ELSA. The $\eta^{\prime}$ mesons have been identified via the $\eta^{\prime} \rightarrow \pi^{0} \pi^{0}\eta \rightarrow 6 \gamma$ decay registered with the CBELSA/TAPS detector system. Coincident protons have been identified in the MiniTAPS BaF2 array at polar angles of $2^{\circ} \le \theta_{p} \le 11^{\circ}$ . Under these kinematic constraints the $\eta^{\prime}$ mesons are produced with relatively low kinetic energy ( $\approx 150$ MeV) since the coincident protons take over most of the momentum of the incident-photon beam. For the C-target this allows the determination of the real part of the $\eta^{\prime}$ -carbon potential at low meson momenta by comparing with collision model calculations of the $\eta^{\prime}$ kinetic energy distribution and excitation function. Fitting the latter data for $\eta^{\prime}$ mesons going backwards in the center-of-mass system yields a potential depth of $V = -(44 \pm 16(stat) \pm 15(syst))$ MeV, consistent with earlier determinations of the potential depth in inclusive measurements for average $\eta^{\prime}$ momenta of $\approx 1.1$ GeV/c. Within the experimental uncertainties, there is no indication of a momentum dependence of the $\eta^{\prime}$ -carbon potential. The LH2 data, taken as a reference to check the data analysis and the model calculations, provide differential and integral cross sections in good agreement with previous results for $\eta^{\prime}$ photoproduction off the free proton.

Quasi-free photoproduction of $ \eta{^\prime}$ -mesons off nucleons bound in the deuteron has been measured with the combined Crystal Barrel - TAPS detector. The experiment was done at a tagged photon beam of the ELSA electron accelerator in Bonn for incident photon energies from the production threshold up to 2.5GeV. The $ \eta{^\prime}$ -mesons have been detected in coincidence with recoil protons and recoil neutrons. The quasi-free proton data are in good agreement with the results for free protons, indicating that nuclear effects have no significant impact. The coincidence with recoil neutrons provides the first data for the $ \gamma$ n $ \rightarrow$ n $ \eta{^\prime}$ reaction. In addition, also first estimates for coherent $ \eta{^\prime}$ -production off the deuteron have been obtained. In agreement with model predictions, the total cross-section for this channel is found to be very small, at most at the level of a few nb. The data are compared to model calculations taking into account contributions from nucleon resonances and t -channel exchanges

Photoproduction of $\eta$ mesons off $^{12}$C, $^{40}$Ca, $^{93}$Nb, and $^{nat}$Pb nuclei has been measured with a tagged photon beam with energies between 0.6 and 2.2 GeV. The experiment was performed at the Bonn ELSA accelerator with the combined setup of the Crystal Barrel and TAPS calorimeters. It aimed at the in-medium properties of the S$_{11}$(1535) nucleon resonance and the study of the absorption properties of nuclear matter for $\eta$ mesons. Careful consideration was given to contributions from $\eta\pi$ final states and secondary production mechanisms of $\eta$-mesons e.g. from inelastic $\pi N$ reactions of intermediate pions. The analysis of the mass number scaling shows that the nuclear absorption cross section $\sigma_{N\eta}$ for $\eta$ mesons is constant over a wide range of the $\eta$ momentum. The comparison of the excitation functions to data off the deuteron and to calculations in the framework of a BUU-model show no unexplained in-medium modifications of the S$_{11}$(1535)., Comment: Accepted for publication in Eur. Phys. J. A

Because they provide a high density and diversity of vertebrate species, small water pools and shaded environments, zoological gardens offer ideal living conditions for numerous mosquito species. Depending on their host preferences and vector competencies, these species may be able to transmit pathogens between native and non-adapted exotic blood host species, thereby causing morbidity and mortality among valuable zoo animals. To determine the extent to which native mosquito species feed on captive and wild animals, as well as on humans, in two German zoological gardens, mosquitoes were collected over two seasons by trapping and aspirating. A total of 405 blood-fed specimens belonging to 16 mosquito taxa were collected. Genetic bloodmeal analysis revealed 56 host species, mainly representing mammals of the zoo animal population, including exotic species previously not known as blood hosts of the mosquito species collected. These results indicate opportunistic feeding patterns with low host-specificity in the analysed mosquitoes, although these could be grouped, according to their bloodmeals, into 'amphibian-', 'non-human mammal-' and 'non-human mammal and human-' feeding species. As the blood-feeding preferences of vector-competent mosquito species are major determinants of vector capacity, information on the blood-feeding behaviour of mosquitoes in zoos is crucial to the success of targeted vector management.

The BGO-OD experiment at the ELSA accelerator facility uses an energy tagged bremstrahlung photon beam to investigate the excitation spectrum of the nucleon. The setup consists of a highly segmented BGO calorimeter surrounding the target, with a particle tracking magnetic spectrometer at forward angles. BGO-OD is ideal for investigating the photoproduction of hadrons of non-zero strangeness. The high momentum resolution at forward angles covers a kinematic region where t-channel exchange mechanisms play a dominant role. Access to this low momentum transfer region also allows the investigation of degrees of freedom not derived from constituent quark models, for example, the role of (vector) meson - baryon interactions and dynamically generated states in photoproduction reactions. Data taking for the first part of an extensive physics programme is complete and preliminary results and ongoing analysis are presented. (Less)

The first measurements of the beam-target-helicity-asymmetries $E$ and $G$ in the photoproduction of $\omega$-mesons off protons at the CBELSA/TAPS experiment are reported. $E$ ($G$) was measured using circularly (linearly) polarised photons and a longitudinally polarised target. $E$ was measured over the photon energy range from close to threshold ($E_\gamma = 1108$~MeV) to $E_\gamma = 2300$~MeV and $G$ at a single energy interval of $1108 < E_\gamma, Comment: 6 pages, 6 figures

Data on the polarization observables T, P, and H for the reaction gamma p -> p pi(0) are reported. Compared to earlier data from other experiments, our data are more precise and extend the covered range in energy and angle substantially. The results were extracted from azimuthal asymmetries measured using a transversely polarized target and linearly polarized photons. The data were taken at the Bonn electron stretcher accelerator ELSA with the CBELSA/TAPS detector. Within the Bonn-Gatchina partial wave analysis, the new polarization data lead to a significant narrowing of the error band for the multipoles for neutral-pion photoproduction. (C) 2015 The Authors. Published by Elsevier B.V.

Photoproduction of two neutral pions off the proton is studied using linearly polarized photons, and the polarization observables I-s and I-c are measured for the first time. These two observables are unique to multi-meson final states; they characterize correlations between the linear photon polarization and the direction of outgoing single particles in photoproduction of three-body final states. The I-s and I-c distributions suggest that, in the 1.8 to 2.0 GeV mass region, the N(1520) 3/2(-)pi intermediate state is reached with reaction dynamics consistent with a dominant J(P) = 3/2(+) wave. These data are included in the Bonn-Gatchina (BnGa) partial wave analysis which is based on a large variety of data; the analysis confirms a significant contribution from the reaction chain gamma p -> N(1900)3/2(+) -> N(1520)3/2(-)pi(0) -> p pi(0)pi(0). (C) 2015 The Authors. Published by Elsevier B.V.

Otolith chemistry is often useful for tracking provenance of fishes, as well as examining migration histories. Whereas elements such as strontium and barium correlate well with salinity and temperature, experiments that examine manganese uptake as a function of these parameters have found no such correlation. Instead, dissolved manganese is available as a redox product, and as such, is indicative of low-oxygen conditions. Here we present evidence for that mechanism in a range of habitats from marine to freshwater, across species, and also present ancillary proxies that support the mechanism as well. For example, iodine is redox-sensitive and varies inversely with Mn; and sulfur stable isotope ratios provide evidence of anoxic sulfate reduction in some circumstances. Further, S may be incorporated trophically whereas other elements appear to be taken up directly from water. This research suggests a potential means to identify individual fish exposure to hypoxia, over entire lifetimes. With further testing and understanding, in the future fish may be able to be used as “mobile monitors” of hypoxic conditions.