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Potyvirus genus is one of the most studied among the plant virus group. It is worldwide distributed and affects different hosts and solanaceus crops. The aims of this work was to determinate the potyvirus incidence in agroeconomical important solanaceus crop in the west zones of Cuba. 1120 young leaves of Solanum lycopersicum, Capsicum annuum and Nicotiana tabacum were collected and analyzed by indirect ELISA, using monoclonal antibodies. Among 34 and 75 % of the plant material was positive. Highest incidence value was detected on San Juan y Martínez and San Antonio de los Baños districts while the lowest values was registered in Güira de Melena [ABSTRACT FROM AUTHOR]

This work aims to provide a current overview about associated diazotrophic bacteria with economically important crops, emphasizing on their diversity, methods for isolation and classification, as well as their potential use as microbial inoculants. Diazotrophic associative bacteria belong to numerous genres of alpha- and beta-proteobacteria. The methods used for studying the diversity of associative diazotrophs include: the inoculation on selective growth media, physiological profile of microbial communities and molecular methods. The diazotrophic bacteria, in addition to the nitrogen contribution made through biological fixation, can directly promote the growth of plants by other mechanisms of action. The production and application of bio-fertilizers requires basic research, ranging from microbiology, ecology and molecular biology. [ABSTRACT FROM AUTHOR]