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In light of the global spread of the new human coronavirus SARS-CoV-2, identified for the first time during an outbreak of pneumonia cases in Wuhan, China, a number of forensic autopsies will be performed on potentially infected corpses. The main aim of this document is to provide safety recommendations to perform forensic autopsies. Moreover, the forensic pathologist sometimes is the first physician facing to the death of a SARSCOV-2 infected subject. In this case, a comprehensive knowledge of SARS-COV-2 clinical and pathological features, may be of the utmost importance not only to timely pose the suspect of infection but also to timely provide safety measures and adequate post-mortem sampling. After summarizing clinical and pathological features of SARSCOV- 2 infection, information about the related health and safety issues will be provided. The autopsy facility must be Biosafety Level 3 and negative pressure with 6-12 air changes per hour should be provided in the autopsy room. Moreover, personal protective equipment for individual protection must guarantee protection both from direct contact and aerosol transmission. Furthermore, clinical information, relevant to pose the suspect of infection before and during the autopsy, will be focused in a dedicated section. Finally, the autopsy procedure will be illustrated together with the microbiological and histopathological sampling activity, recommended not only for diagnostic purposes but also for scientific studies. SARS-CoV-2 recommendations dedicated to the forensic setting seem of the utmost importance. Indeed, the forensic pathologist might be claimed not only to establish the cause of death, but also to solve a number of further issues, such as: the assessment of malpractice and medical liability, complicated by the rapid evolution of the state of the art; the potential contributory role of SARS-CoV-2 infection in the manner of death, for example, cases of suicide have been reported in infected patients; to reconstruct retrospectively the time and transmission modality of the infection in a specific subject, aimed for example to evaluate the liability to third parties especially for infections occurred in fragile patients living in nursing homes for elderly or disabled people.

Mast cells (MCs) are derived from bone marrow precursors and are immune cells involved in acute and chronic inflammation. MCs are ubiquitous and play a crucial role in innate and acquired immunity. They are activated through cross-linking of their surface high affinity receptors (FcεRI), leading to immediate secretion of stored inflammatory mediators, and late production and release of pro-inflammatory cytokines/chemokines without degranulation. Therefore, MCs are important in inflammatory responses. Members of the interleukin (IL)-1 cytokine family, such as IL-1 and IL-33, and various antigens markedly increase IL-1 and tumor necrosis factor (TNF) expression and secretion from MCs. One of the latest cytokines is IL-33, an IL-1 family member acting via its ST2/IL-1R4, which has been shown to regulate MCs. IL-1 and IL-33 are cytokines found to be implicated in many inflammatory disorders including rheumatoid arthritis, atherosclerosis and psoriasis. In general, IL-1 family member cytokines play a pro-inflammatory role and increase the pathological state. IL-37 is a member of the IL-1 family with anti-inflammatory activity through inhibition of pro-inflammatory cytokines. IL-37 particularly suppresses IL-1-mediated innate inflammatory response, but also acts on the acquired immune response. IL-37 is activated by pro-inflammatory agents and cytokines, playing a protective role against inflammation. This cytokine is a natural regulator of immunity and is a therapeutic promise against inflammatory diseases. Since IL-1 is produced by and activates MCs to release IL-33 and TNF, here we hypothesize that MCs can be inhibited by IL-37 and therefore reduce their pro-inflammatory activity. However, the maturation, transport and secretion of IL-37 remain to be clarified.

Different economies have different propensities to generate inflationary pressures, and different capacity to absorb the strains occasioned by price and wage increases. Because of economic and institutional features of less developed countries, expansionary policies may have to be undertaken in a ‘typical” developing country, more often and to a greater extent than in a ‘typical’ developed country. Even a mild rate of inflation will be damaging under a fixed-exchange-rate system, if domestic prices increase at a faster pace than prices abroad. Chronic balance-of-payments difficulties are confronted by inflationary countries that are attached to a fixed-exchange-rate system. Rather than sporadic, substantial devaluations, periodical mini-devaluations may be advisable in developing countries with a relatively high propensity to inflate.

Molds include all species of microscopic fungi, the spores of which are small molecules, ubiquitous, mostly found in soil with higher rainfall and high humidity, in the atmosphere of urban and rural settings and in decaying vegetation. They originate from pathogenic fungi and have a crucial role in inflammatory response, causing a broad range of diseases. Immune suppressed subjects may develop mycoses caused by opportunistic common pathogenic fungi. Mast cells (MCs) are immune cells involved in the pathophysiology of infected skin, lung, and organs, where there is an increase of angiogenesis. Airways fungi infections can induce allergic lung disease mediated by MCs and other immune cells. In addition, fungal infection may cause and/or aggravate asthma inflammation. Spores are able to navigate in the airways of the lung and can be recognized trough toll-like receptor (TLR) signaling by the innate immune cells including MCs. Activated MCs release preformed mediators including histamine, proteases (tryptase, chimase), pro-inflammatory cytokines/chemokines and they also generate arachidonic acid products. MCs activated by fungi provoke an increases of PGD2 levels and lead to hypersensitivity diseases which present signs such as irritation of the respiratory tract and eyes, recurrent sinusitis, bronchitis, cough and neurological manifestations including fatigue, nausea, headaches and brain fog. Therefore, fungi activate the innate immune response through the TLRs, leading to the release of myeloid differentiation factor 88 (MyD88) which, with a series of cascade reactions, induces the stimulation of AP-1 and NF-kB with subsequent activation of inflammatory IL-1 family members. Here, we report that fungi can activate MCs to secrete pro-inflammatory cytokines which may be inhibited by IL-37, a new anti-inflammatory IL-1 family member.

Migraine is a common painful neurovascular disorder usually associated with several symptoms, such as photophobia, phonophobia, nausea, vomiting and inflammation, and involves immune cells. Mast cells (MCs) are immune cells derived from hematopoietic pluripotent stem cells which migrate and mature close to epithelial, blood vessels, and nerves. In almost all vascularized tissues there are MCs that produce, contain and release biologically active products including cytokines, arachidonic acid compounds, and proteases. In addition, MCs participate in innate and adaptive immune responses. Innate responses in the central nervous system (CNS) occur during neuroinflammatory phenomena, including migraine. Antigens found in the environment have a crucial role in inflammatory response, causing a broad range of diseases including migraine. They can be recognized by several innate immune cells, such as macrophages, microglia, dendritic cells and MCs, which can be activated trough Toll-like receptor (TLR) signaling. MCs reside close to primary nociceptive neurons, associate with nerves, and are capable of triggering local inflammation. MCs are involved in the pathophysiology of various tissues and organs, especially where there is an increase of angiogenesis. Activated MCs release preformed mediators include histamine, heparin, proteases (tryptase, chimase), hydrolases, cathepsin, carboxypeptidases, and peroxidase, and they also generate pro-inflammatory cytokines/chemokines. In addition, activated macrophages, microglia and MCs in the CNS release pro-inflammatory cytokines which provoke an increase of arachidonic acid product levels and lead to migraine and other neurological manifestations including fatigue, nausea, headaches and brain fog. Innate immunity and pro-inflammatory interleukin (IL)-1 cytokine family members can be inhibited by IL-37, a relatively new member of the IL-1 family. In this article, we report that some pro-inflammatory cytokines inducing migraine may be inhibited by IL-37, a natural suppressor of inflammation, and innate and acquired immunity.

In questo capitolo mettiamo a fuoco il profilo di questi quattro spazi di fabbricazione in relazione alle interazioni nei confronti dei quartieri in cui si trovano. La questione che qui solleviamo va al di là della comunità di pratiche che corrisponde ai maker. Ci si chiede piuttosto se i nuovi spazi della fabbricazione digitale riescono a instaurare delle relazioni di prossimità con l’intorno urbano nel quale sono localizzati al fine di contribuire, in un modo o nell’altro, alla rigenerazione della città a partire dalla scala del quartiere.

Pur sottolineando che i nuovi spazi della fabbricazione digitale non si pongono nei confronti della rigenerazione urbana in quanto attori intenzionali e propositivi, la nostra attenzione è rivolta in questo capitolo a comprendere se attraverso le relazioni di prossimità i laboratori maker contribuiscono a trasformare la città a partire dal quartiere. Ad oggi, a questo riguardo, si evidenziano infatti delle contraddizioni.

In this work, the synthesis, characterization, and application of novel parabens imprinted polymers as highly selective solid-phase extraction (SPE) sorbents have been reported. The imprinted polymers were created using sol&ndash, gel molecular imprinting process. All the seven parabens were considered herein in order to check the phase selectivity. By means of a validated HPLC-photodiode array detector (PDA) method all seven parabens were resolved in a single chromatographic run of 25 min. These SPE sorbents, in-house packed in SPE empty cartridges, were first characterized in terms of extraction capability, breakthrough volume, retention volume, hold-up volume, number of theoretical plates, and retention factor. Finally, the device was applied to a real urine sample to check the method feasibility on a very complex matrix. The new paraben imprinted SPE sorbents, not yet present in the literature, potentially encourage the development of novel molecularly imprinted polymers (MIPs) to enhance the extraction efficiency, and consequently the overall analytical performances, when the trace quantification is required.