39 results on '"DE-JIA LI"'
Search Results
2. FOXC1-mediated LINC00301 facilitates tumor progression and triggers an immune-suppressing microenvironment in non-small cell lung cancer by regulating the HIF1α pathway
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Cheng-Cao Sun, Wei Zhu, Shu-Jun Li, Wei Hu, Jian Zhang, Yue Zhuo, Han Zhang, Juan Wang, Yu Zhang, Shao-Xin Huang, Qi-Qiang He, and De-Jia Li
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LINC00301 ,ELL protein-associated factor 2 (EAF2) ,Non-small cell lung cancer (NSCLC) ,Enhancer of zeste homolog 2 (EZH2) ,Regulatory T cells (Treg) ,Hypoxia-inducible factor 1 α (HIF1α) ,Medicine ,Genetics ,QH426-470 - Abstract
Abstract Background Long non-coding RNAs (lncRNAs) are extensively intricate in the tumorigenesis and metastasis of various cancer types. Nevertheless, the detailed molecular mechanisms of lncRNA in non-small cell lung cancer (NSCLC) still remain mainly undetermined. Methods qPCR was performed to verify LINC00301 expression in NSCLC clinical specimens or cell lines. Fluorescence in situ hybridization (FISH) was conducted to identify the localization of LINC00301 in NSCLC cells. Chromatin immunoprecipitation (ChIP) was subjected to validate the binding activity between FOXC1 and LINC00301 promoters. RNA immunoprecipitation (RIP) was performed to explore the binding activity between LINC00301 and EZH2. RNA pull-down followed by dot-blot, protein domain mapping, and RNA electrophoresis mobility shift assay (EMSA) were conducted to identify the detailed binding regions between LINC00301 and EZH2. Alpha assay was conducted to quantitatively assess the interaction between LINC00301 and EZH2. Results LINC00301 is highly expressed in NSCLC and closely corelated to its prognosis by analyzing the relationship between differentially expressed lncRNAs and prognosis in NSCLC samples. in vitro and in vivo experiments revealed that LINC00301 facilitates cell proliferation, releases NSCLC cell cycle arrest, promotes cell migration and invasion, and suppresses cell apoptosis in NSCLC. In addition, LINC00301 increases regulatory T cell (Treg) while decreases CD8+ T cell population in LA-4/SLN-205-derived tumors through targeting TGF-β. The transcription factor FOXC1 mediates LINC00301 expression in NSCLC. Bioinformatics prediction and in vitro experiments indicated that LINC00301 (83–123 nucleotide [nt]) can directly bind to the enhancer of zeste homolog 2 (EZH2) (612–727 amino acid [aa]) to promote H3K27me3 at the ELL protein-associated factor 2 (EAF2) promoter. EAF2 directly binds and stabilizes von Hippel–Lindau protein (pVHL), so downregulated EAF2 augments hypoxia-inducible factor 1 α (HIF1α) expression by regulating pVHL in NSCLC cells. Moreover, we also found that LINC00301 could function as a competing endogenous RNA (ceRNA) against miR-1276 to expedite HIF1α expression in the cytoplasm of NSCLC cells. Conclusions In summary, our present research revealed the oncogenic roles of LINC00301 in clinical specimens as well as cellular and animal experiments, illustrating the potential roles and mechanisms of the FOXC1/LINC00301/EZH2/EAF2/pVHL/HIF1α and FOXC1/LINC00301/miR-1276/HIF1α pathways, which provides novel insights and potential theraputic targets to NSCLC.
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- 2020
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3. Comprehensive landscape of extracellular vesicle-derived RNAs in cancer initiation, progression, metastasis and cancer immunology
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Wei Hu, Cong Liu, Zhuo-Yue Bi, Qun Zhou, Han Zhang, Lin-Lin Li, Jian Zhang, Wei Zhu, Yang-Yi-Yan Song, Feng Zhang, Hui-Min Yang, Yong-Yi Bi, Qi-Qiang He, Gong-Jun Tan, Cheng-Cao Sun, and De-Jia Li
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Extracellular vesicle ,Exosome ,Microvesicle ,Micro RNA ,Long non-coding RNA ,Circular RNA ,Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,RC254-282 - Abstract
Abstract Extracellular vesicles (EVs), a class of heterogeneous membrane vesicles, are generally divided into exosomes and microvesicles on basis of their origination from the endosomal membrane or the plasma membrane, respectively. EV-mediated bidirectional communication among various cell types supports cancer cell growth and metastasis. EVs derived from different cell types and status have been shown to have distinct RNA profiles, comprising messenger RNAs and non-coding RNAs (ncRNAs). Recently, ncRNAs have attracted great interests in the field of EV-RNA research, and growing numbers of ncRNAs ranging from microRNAs to long ncRNAs have been investigated to reveal their specific functions and underlying mechanisms in the tumor microenvironment and premetastatic niches. Emerging evidence has indicated that EV-RNAs are essential functional cargoes in modulating hallmarks of cancers and in reciprocal crosstalk within tumor cells and between tumor and stromal cells over short and long distance, thereby regulating the initiation, development and progression of cancers. In this review, we discuss current findings regarding EV biogenesis, release and interaction with target cells as well as EV-RNA sorting, and highlight biological roles and molecular mechanisms of EV-ncRNAs in cancer biology.
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- 2020
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4. Correction to: FOXC1-mediated LINC00301 facilitates tumor progression and triggers an immune-suppressing microenvironment in non-small cell lung cancer by regulating the HIF1α pathway
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Cheng-Cao Sun, Wei Zhu, Shu-Jun Li, Wei Hu, Jian Zhang, Yue Zhuo, Han Zhang, Juan Wang, Yu Zhang, Shao-Xin Huang, Qi-Qiang He, and De-Jia Li
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Medicine ,Genetics ,QH426-470 - Abstract
An amendment to this paper has been published and can be accessed via the original article.
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- 2021
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5. Expression and Prognosis Analyses of Runt-Related Transcription Factor Family in Human Leukemia
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Cheng-Cao Sun, Shu-Jun Li, Zhen-Long Chen, Guang Li, Qian Zhang, and De-Jia Li
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Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,RC254-282 - Abstract
Despite advances in early diagnosis and treatment, cancer remains the major reason for mortality worldwide. The Runt-related transcription factor (RUNX) family has been reported to participate in diverse human diseases. However, little is known about their expression and prognostic values in human leukemia. Herein, we conducted a detailed cancer versus normal analysis. The mRNA expression levels of the RUNX family in various kinds of cancers, including leukemia, were analyzed via the ONCOMINE and GEPIA (Gene Expression Profiling Interactive Analysis) databases. We observed that the mRNA expression levels of RUNX1, RUNX2, and RUNX3 were all increased in most cancers compared with normal tissues, especially in leukemia. Moreover, the expression levels of RUNX1, RUNX2, and RUNX3 are also highly expressed in almost all cancer cell lines, particularly in acute myeloid leukemia (AML) cell lines, analyzed by Cancer Cell Line Encyclopedia (CCLE) and European Bioinformatics Institute (EMBL-EBI) databases. Further, the LinkedOmics and GEPIA databases were used to evaluate the prognostic values. In survival analyses based on LinkedOmics, higher expression of RUNX1 and RUNX2 indicated a better overall survival (OS), but with no significance, whereas increased RUNX3 revealed a poor OS in leukemia. In addition, the GEPIA dataset was also used to perform survival analyses, and results manifested that the expression of RUNX1 and RUNX2 had no remarkable correction with OS in leukemia, but it showed highly expressed RUNX3 was significantly related with poor OS in leukemia. In conclusion, the RUNX family showed significant expression differences between cancer and normal tissues, especially leukemia, and RUNX3 could be a promising prognostic biomarker for leukemia. Keywords: Runt-related transcription factor, leukemia, ONCOMINE, Cancer Cell Line Encyclopedia
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- 2019
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6. Association Between ABO Blood Group System and COVID-19 Susceptibility in Wuhan
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Qian Fan, Wei Zhang, Bo Li, De-Jia Li, Jian Zhang, and Fang Zhao
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ABO blood group system ,COVID-19 ,association analysis ,female ,lymphocyte count ,Microbiology ,QR1-502 - Abstract
Background: The ABO blood group system has been associated with multiple infectious diseases, including hepatitis B, dengue haemorrhagic fever and so on. Coronavirus disease 2019 (COVID-19) is a new respiratory infectious disease and the relationship between COVID-19 and ABO blood group system needs to be explored urgently.Methods: A hospital-based case-control study was conducted at Zhongnan Hospital of Wuhan University from 1 January 2020 to 5 March 2020. A total of 105 COVID-19 cases and 103 controls were included. The blood group frequency was tested with the chi-square statistic, and odds ratios (ORs) with 95% confidence intervals (CIs) were calculated between cases and controls. In addition, according to gender, the studied population was divided into two subgroups, and we assessed the association between cases and controls by gender. Finally, considering lymphopenia as a feature of COVID-19, the relationship between the ABO blood group and the lymphocyte count was determined in case samples.Results: The frequencies of blood types A, B, AB, and O were 42.8, 26.7, 8.57, and 21.9%, respectively, in the case group. Association analysis between the ABO blood group and COVID-19 indicated that there was a statistically significant difference for blood type A (P = 0.04, OR = 1.33, 95% CI = 1.02–1.73) but not for blood types B, AB or O (P = 0.48, OR = 0.90, 95% CI = 0.66–1.23; P = 0.61, OR = 0.88, 95% CI = 0.53–1.46; and P = 0.23, OR = 0.82, 95% CI = 0.58–1.15, respectively). An analysis stratified by gender revealed that the association was highly significant between blood type A in the female subgroup (P = 0.02, OR = 1.56, 95% CI = 1.08–2.27) but not in the male subgroup (P = 0.51, OR = 1.14, 95% CI = 0.78–1.67). The average level of lymphocyte count was the lowest with blood type A in patients, however, compared with other blood types, there was still no significant statistical difference.Conclusions: Our findings provide epidemiological evidence that females with blood type A are susceptible to COVID-19. However, these research results need to be validated in future studies.
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- 2020
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7. The lncRNA PDIA3P Interacts with miR-185-5p to Modulate Oral Squamous Cell Carcinoma Progression by Targeting Cyclin D2
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Cheng-Cao Sun, Ling Zhang, Guang Li, Shu-Jun Li, Zhen-Long Chen, Yun-Feng Fu, Feng-Yun Gong, Tao Bai, Ding-Yu Zhang, Qing-Ming Wu, and De-Jia Li
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protein disulfide isomerase family A member 3 pseudogene 1 ,PDIA3P ,miR-185-5p ,cyclin D2 ,oral squamous cell carcinoma ,OSCC ,tumorigenesis ,Therapeutics. Pharmacology ,RM1-950 - Abstract
Long noncoding RNAs (lncRNAs) are emerging as important regulators during tumorigenesis by serving as competing endogenous RNAs (ceRNAs). In this study, the qRT-PCR results indicated that the lncRNA protein disulfide isomerase family A member 3 pseudogene 1 (PDIA3P) was overexpressed in oral squamous cell carcinoma (OSCC) and decreased the survival rate of OSCC patients. CCK-8 and clonal colony formation assays were used to detect the effects of PDIA3P on proliferation. Results revealed that silencing PDIA3P by small interfering RNA (siRNA) inhibited OSCC cell proliferation and repressed tumor growth and reduced the expression of proliferation antigen Ki-67 in vivo. Furthermore, the interaction between PDIA3P and miRNAs was then analyzed by qRT-PCR and luciferase reporter gene assay. We found that PDIA3P negatively regulated miR-185-5p in OSCC cells. Simultaneously, we found that silencing PDIA3P by siRNA suppressed proliferation via miR-185-5p in OSCC cells. Moreover, silencing PDIA3P by siRNA inhibited CCND2 protein (no influence on mRNA levels) expression via miR-185-5p in OSCC cells, and CCND2 facilitated cell proliferation of SCC4 and SCC15 cells induced by sh-PDIA3P#1. Therefore, our study demonstrated that PDIA3P may be a therapeutic target for the treatment of OSCC.
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- 2017
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8. miR-206/133b Cluster: A Weapon against Lung Cancer?
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Jing-Yu Pan, Cheng-Cao Sun, Zhuo-Yue Bi, Zhen-Long Chen, Shu-Jun Li, Qing-Qun Li, Yu-Xuan Wang, Yong-Yi Bi, and De-Jia Li
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Therapeutics. Pharmacology ,RM1-950 - Abstract
Lung cancer is a deadly disease that ends numerous lives around the world. MicroRNAs (miRNAs) are a group of non-coding RNAs involved in a variety of biological processes, such as cell growth, organ development, and tumorigenesis. The miR-206/133b cluster is located on the human chromosome 6p12.2, which is essential for growth and rebuilding of skeletal muscle. The miR-206/133b cluster has been verified to be dysregulated and plays a crucial role in lung cancer. miR-206 and miR-133b participate in lung tumor cell apoptosis, proliferation, migration, invasion, angiogenesis, drug resistance, and cancer treatment. The mechanisms are sophisticated, involving various target genes and molecular pathways, such as MET, EGFR, and the STAT3/HIF-1α/VEGF signal pathway. Hence, in this review, we summarize the role and potential mechanisms of the miR-206/133b cluster in lung cancer. Keywords: lung cancer, miR-206/133b cluster, miR-206, miR-133b
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- 2017
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9. miR-134: A Human Cancer Suppressor?
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Jing-Yu Pan, Feng Zhang, Cheng-Cao Sun, Shu-Jun Li, Guang Li, Feng-Yun Gong, Tao Bo, Jing He, Rui-Xi Hua, Wei-Dong Hu, Zhan-Peng Yuan, Xin Wang, Qi-Qiang He, and De-Jia Li
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Therapeutics. Pharmacology ,RM1-950 - Abstract
MicroRNAs (miRNAs) are small noncoding RNAs approximately 20–25 nt in length, which play crucial roles through directly binding to corresponding 3′ UTR of targeted mRNAs. It has been reported that miRNAs are involved in numerous of diseases, including cancers. Recently, miR-134 has been identified to dysregulate in handles of human cancers, such as lung cancer, glioma, breast cancer, colorectal cancer, and so on. Increasing evidence indicates that miR-134 is essential for human carcinoma and participates in tumor cell proliferation, apoptosis, invasion and metastasis, drug resistance, as well as cancer diagnosis, treatment, and prognosis. Nevertheless, its roles in human cancer are still ambiguous, and its mechanisms are sophisticated as well, referring to a variety of targets and signal pathways, such as STAT5B, KRAS, MAPK/ERK signal pathway, Notch pathway, etc. Herein, we review the crucial roles of miR-134 in scores of human cancers via analyzing latest investigations, which might provide evidence for cancer diagnose, treatment, prognosis, or further investigations. Keywords: miR-134, human cancer, microRNA
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- 2017
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10. The Novel miR-9600 Suppresses Tumor Progression and Promotes Paclitaxel Sensitivity in Non–small-cell Lung Cancer Through Altering STAT3 Expression
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Cheng-Cao Sun, Shu-Jun Li, Feng Zhang, Ya-Dong Zhang, Zhen-Yu Zuo, Yong-Yong Xi, Liang Wang, and De-Jia Li
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drug resistance ,miR-9600 ,non–small-cell lung cancer ,STAT3 ,tumorigenesis ,Therapeutics. Pharmacology ,RM1-950 - Abstract
MicroRNAs have been identified to be involved in center stage of cancer biology. They accommodate cell proliferation and migration by negatively regulate gene expression either by hampering the translation of targeted mRNAs or by promoting their degradation. We characterized and identified the novel miR-9600 and its target in human non–small-cell lung cancer (NSCLC). Our results demonstrated that the miR-9600 were downregulated in NSCLC tissues and cells. It is confirmed that signal transducer and activator of transcription 3 (STAT3), a putative target gene, is directly inhibited by miR-9600. The miR-9600 markedly suppressed the protein expression of STAT3, but with no significant influence in corresponding mRNA levels, and the direct combination of miR-9600 and STAT3 was confirmed by a luciferase reporter assay. miR-9600 inhibited cell growth, hampered expression of cell cycle-related proteins and inhibited cell migration and invasion in human NSCLC cell lines. Further, miR-9600 significantly suppressed tumor growth in nude mice. Similarly, miR-9600 impeded tumorigenesis and metastasis through directly targeting STAT3. Furthermore, we identified that miR-9600 augmented paclitaxel and cisplatin sensitivity by downregulating STAT3 and promoting chemotherapy-induced apoptosis. These data demonstrate that miR-9600 might be a useful and novel therapeutic target for NSCLC.
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- 2016
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11. Long Intergenic Noncoding RNA 00511 Acts as an Oncogene in Non–small-cell Lung Cancer by Binding to EZH2 and Suppressing p57
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Cheng-Cao Sun, Shu-Jun Li, Guang Li, Rui-Xi Hua, Xu-Hong Zhou, and De-Jia Li
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enhancer of zeste homolog 2 ,long noncoding RNA LINC00511 ,p57 ,non–small-cell lung cancer ,polycomb repressive complex 2 ,tumorigenesis ,Therapeutics. Pharmacology ,RM1-950 - Abstract
Long noncoding RNAs (lncRNAs) play crucial roles in carcinogenesis. However, the function and mechanism of lncRNAs in human non–small-cell lung cancer (NSCLC) are still remaining largely unknown. Long intergenic noncoding RNA 00511 (LINC00511) has been found to be upregulated and acts as an oncogene in breast cancer, but little is known about its expression pattern, biological function and underlying mechanism in NSCLC. Herein, we identified LINC00511 as an oncogenic lncRNA by driving tumorigenesis in NSCLC. We found LINC00511 was upregulated and associated with oncogenesis, tumor size, metastasis, and poor prognosis in NSCLC. Moreover, LINC00511 affected cell proliferation, invasiveness, metastasis, and apoptosis in multiple NSCLC cell lines. Mechanistically, LINC00511 bound histone methyltransferase enhancer of zeste homolog 2 ((EZH2, the catalytic subunit of the polycomb repressive complex 2 (PRC2), a highly conserved protein complex that regulates gene expression by methylating lysine 27 on histone H3), and acted as a modular scaffold of EZH2/PRC2 complexes, coordinated their localization, and specified the histone modification pattern on the target genes, including p57, and consequently altered NSCLC cell biology. Thus, LINC00511 is mechanistically, functionally, and clinically oncogenic in NSCLC. Targeting LINC00511 and its pathway may be meaningful for treating patients with NSCLC.
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- 2016
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12. Comprehensive landscape of extracellular vesicle-derived RNAs in cancer initiation, progression, metastasis and cancer immunology
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Qi-qiang He, Zhuoyue Bi, Wei Zhu, Yongyi Bi, Cong Liu, Jian Zhang, Cheng-Cao Sun, Lin-Lin Li, Wei Hu, Han Zhang, Qun Zhou, Feng Zhang, Hui-Min Yang, Gong-Jun Tan, Yang-Yi-Yan Song, and De-Jia Li
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0301 basic medicine ,Cancer Research ,Micro RNA ,RNA, Untranslated ,Review ,Biology ,Exosome ,lcsh:RC254-282 ,Extracellular Vesicles ,03 medical and health sciences ,0302 clinical medicine ,Microvesicle ,Neoplasms ,microRNA ,Biomarkers, Tumor ,Animals ,Humans ,RNA, Messenger ,Neoplasm Metastasis ,Premetastatic niche ,Circular RNA ,Cancer ,Cancer immunology ,Tumor microenvironment ,Extracellular vesicle ,lcsh:Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,Long non-coding RNA ,Microvesicles ,Cell biology ,MicroRNAs ,030104 developmental biology ,Oncology ,030220 oncology & carcinogenesis ,Disease Progression ,Molecular Medicine - Abstract
Extracellular vesicles (EVs), a class of heterogeneous membrane vesicles, are generally divided into exosomes and microvesicles on basis of their origination from the endosomal membrane or the plasma membrane, respectively. EV-mediated bidirectional communication among various cell types supports cancer cell growth and metastasis. EVs derived from different cell types and status have been shown to have distinct RNA profiles, comprising messenger RNAs and non-coding RNAs (ncRNAs). Recently, ncRNAs have attracted great interests in the field of EV-RNA research, and growing numbers of ncRNAs ranging from microRNAs to long ncRNAs have been investigated to reveal their specific functions and underlying mechanisms in the tumor microenvironment and premetastatic niches. Emerging evidence has indicated that EV-RNAs are essential functional cargoes in modulating hallmarks of cancers and in reciprocal crosstalk within tumor cells and between tumor and stromal cells over short and long distance, thereby regulating the initiation, development and progression of cancers. In this review, we discuss current findings regarding EV biogenesis, release and interaction with target cells as well as EV-RNA sorting, and highlight biological roles and molecular mechanisms of EV-ncRNAs in cancer biology.
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- 2020
13. Correction to: FOXC1-mediated LINC00301 facilitates tumor progression and triggers an immune-suppressing microenvironment in non-small cell lung cancer by regulating the HIF1α pathway
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Han Zhang, Shu-Jun Li, Jian Zhang, Shao-Xin Huang, Wei Zhu, Juan Wang, Cheng-Cao Sun, De-Jia Li, Yue Zhuo, Wei Hu, Qi-Qiang He, and Yu Zhang
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0301 basic medicine ,lcsh:QH426-470 ,T cell ,non-small cell lung cancer (NSCLC) ,lcsh:Medicine ,medicine.disease_cause ,03 medical and health sciences ,0302 clinical medicine ,Genetics ,medicine ,Electrophoretic mobility shift assay ,Molecular Biology ,Transcription factor ,Genetics (clinical) ,Chemistry ,Competing endogenous RNA ,Cell growth ,lcsh:R ,medicine.disease ,lcsh:Genetics ,030104 developmental biology ,medicine.anatomical_structure ,030220 oncology & carcinogenesis ,Cancer research ,Molecular Medicine ,Carcinogenesis ,Chromatin immunoprecipitation - Abstract
Long non-coding RNAs (lncRNAs) are extensively intricate in the tumorigenesis and metastasis of various cancer types. Nevertheless, the detailed molecular mechanisms of lncRNA in non-small cell lung cancer (NSCLC) still remain mainly undetermined. qPCR was performed to verify LINC00301 expression in NSCLC clinical specimens or cell lines. Fluorescence in situ hybridization (FISH) was conducted to identify the localization of LINC00301 in NSCLC cells. Chromatin immunoprecipitation (ChIP) was subjected to validate the binding activity between FOXC1 and LINC00301 promoters. RNA immunoprecipitation (RIP) was performed to explore the binding activity between LINC00301 and EZH2. RNA pull-down followed by dot-blot, protein domain mapping, and RNA electrophoresis mobility shift assay (EMSA) were conducted to identify the detailed binding regions between LINC00301 and EZH2. Alpha assay was conducted to quantitatively assess the interaction between LINC00301 and EZH2. LINC00301 is highly expressed in NSCLC and closely corelated to its prognosis by analyzing the relationship between differentially expressed lncRNAs and prognosis in NSCLC samples. in vitro and in vivo experiments revealed that LINC00301 facilitates cell proliferation, releases NSCLC cell cycle arrest, promotes cell migration and invasion, and suppresses cell apoptosis in NSCLC. In addition, LINC00301 increases regulatory T cell (Treg) while decreases CD8+ T cell population in LA-4/SLN-205-derived tumors through targeting TGF-β. The transcription factor FOXC1 mediates LINC00301 expression in NSCLC. Bioinformatics prediction and in vitro experiments indicated that LINC00301 (83–123 nucleotide [nt]) can directly bind to the enhancer of zeste homolog 2 (EZH2) (612–727 amino acid [aa]) to promote H3K27me3 at the ELL protein-associated factor 2 (EAF2) promoter. EAF2 directly binds and stabilizes von Hippel–Lindau protein (pVHL), so downregulated EAF2 augments hypoxia-inducible factor 1 α (HIF1α) expression by regulating pVHL in NSCLC cells. Moreover, we also found that LINC00301 could function as a competing endogenous RNA (ceRNA) against miR-1276 to expedite HIF1α expression in the cytoplasm of NSCLC cells. In summary, our present research revealed the oncogenic roles of LINC00301 in clinical specimens as well as cellular and animal experiments, illustrating the potential roles and mechanisms of the FOXC1/LINC00301/EZH2/EAF2/pVHL/HIF1α and FOXC1/LINC00301/miR-1276/HIF1α pathways, which provides novel insights and potential theraputic targets to NSCLC.
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- 2021
14. Retraction Notice to: Comprehensive Analysis of the Expression and Prognosis for E2Fs in Human Breast Cancer
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Cheng-Cao Sun, Shu-Jun Li, Wei Hu, Jian Zhang, Qun Zhou, Cong Liu, Lin-Lin Li, Yi-Yan Songyang, Feng Zhang, Zhen-Long Chen, Guang Li, Zhuo-Yue Bi, Yong-Yi Bi, Feng-Yun Gong, Tao Bo, Zhan-Peng Yuan, Wei-Dong Hu, Bo-Tao Zhan, Qian Zhang, Qi-Qiang He, and De-Jia Li
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Pharmacology ,Drug Discovery ,Genetics ,Molecular Medicine ,Molecular Biology - Published
- 2022
15. Roles of miR-200 family members in lung cancer: more than tumor suppressors
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Feng Zhang, Wei Zhu, De-Jia Li, Qun Zhou, Cheng-Chao Sun, Wei Hu, Cong Liu, Lin-Lin Li, Yiyan Songyang, and Yu-Xuan Wang
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0301 basic medicine ,Cancer Research ,Epithelial-Mesenchymal Transition ,Lung Neoplasms ,Apoptosis ,medicine.disease_cause ,Epigenesis, Genetic ,law.invention ,03 medical and health sciences ,Fibrosis ,law ,Carcinoma, Non-Small-Cell Lung ,microRNA ,Humans ,Medicine ,Genes, Tumor Suppressor ,Epigenetics ,Lung cancer ,Gene ,Cell Proliferation ,business.industry ,Cancer ,Cell Differentiation ,General Medicine ,medicine.disease ,Gene Expression Regulation, Neoplastic ,MicroRNAs ,030104 developmental biology ,Oncology ,Disease Progression ,Cancer research ,Suppressor ,business ,Carcinogenesis - Abstract
miRNAs are a class of single-stranded noncoding RNAs, which have no coding potential, but modulate many molecular mechanisms including cancer pathogenesis. miRNAs participate in cell proliferation, differentiation, apoptosis, as well as carcinogenesis or cancer progression, and their involvement in lung cancer has been recently shown. They are suggested to have bidirectional functions on important cancer-related genes so as to enhance or attenuate tumor genesis. Epithelial–mesenchymal transition (EMT) is a fundamental process which contributes to integrity of organogenesis and tissue differentiation as well as tissue repair, organ fibrosis and the progression of carcinoma, and several miRNAs were suggested to form the network regulating EMT in lung cancer, among which, miR-200 family members (miR-200a, miR-200b, miR-200c, miR-429 and miR-141) play crucial roles in the suppression of EMT.
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- 2018
16. Acute effects of ambient air pollution on clinic visits of college students for upper respiratory tract infection in Wuhan, China
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Miaoxuan Zhang, Huan Feng, Wei Zhu, De-Jia Li, Han Zhang, Faxue Zhang, and Chuangxin Wu
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Acute effects ,Male ,China ,Names of the days of the week ,Health, Toxicology and Mutagenesis ,Air pollution ,010501 environmental sciences ,medicine.disease_cause ,complex mixtures ,01 natural sciences ,Environmental health ,Air Pollution ,medicine ,Ambulatory Care ,Environmental Chemistry ,Humans ,Students ,Respiratory Tract Infections ,0105 earth and related environmental sciences ,Pollutant ,Air Pollutants ,Ambient air pollution ,business.industry ,General Medicine ,medicine.disease ,Pollution ,Upper respiratory tract infection ,Increased risk ,Female ,Particulate Matter ,business - Abstract
Ambient air pollutants have been linked to adverse health outcomes, but evidence is still relatively rare in college students. Upper respiratory tract infection (URTI) is a common disease of respiratory system among college students. In this study, we assess the acute effect of air pollution on clinic visits of college students for URTI in Wuhan, China. Data on clinic visits due to URTI were collected from Wuhan University Hospital, meteorological factors (including daily temperature and relative humidity) provided by Wuhan Meteorological Bureau, and air pollutants by Wuhan Environmental Protection Bureau. In the present study, generalized additive model with a quasi-Poisson distribution link function was used to examine the association between ambient air pollutants (fine particulate matter (PM2.5), particulate matter (PM10), sulfur dioxide (SO2), nitrogen dioxide (NO2), and ozone (O3)) and the daily number of clinic visits of college students for URTI at Wuhan University Hospital in Wuhan, China. In the meantime, the model was adjusted for the confounding effects of long-term trends, seasonality, day of the week, public holidays, vacation, and meteorological factors. The best degrees of free in model were selected based on AIC (Akaike Information Criteria). The effect modification by gender was also examined. A total of 44,499 cases with principal diagnosis of URTI were included from January 1, 2016, to December 31, 2018. In single-pollutant models, the largest increment of URTI visits were found at lag 0 day in single-day lags, and the effect values in cumulative lags were greater than those in single-day lags. PM2.5 (0.74% (95%CI: 0.05, 1.44)) at lag 0 day, PM10 (0.61% (95%CI: 0.12, 1.11)) and O3 (1.01% (95%CI: 0.24, 1.79)) at lag 0–1 days, and SO2 (9.18% (95%CI: 3.27, 15.42)) and NO2 (3.40% (95% CI:1.64, 5.19)) at lag 0–3 days were observed to be strongly and significantly associated with clinic visits for URTI. PM10 and NO2 were almost still significantly associated with URTI after controlling for the other pollutants in our two-pollutant models, where the effect value of SO2 after inclusion of O3 appeared to be the largest and the effects of NO2 were also obvious compared with the other pollutants. Subgroups analysis demonstrated that males were more vulnerable to PM10 and O3, while females seemed more vulnerable to exposure to SO2 and NO2. This study implied that short-term exposure to ambient air pollution was associated with increased risk of URTI among college students at Wuhan University Hospital in Wuhan, China. And gaseous pollutants had more negative health impact than solid pollutants. SO2 and NO2 were the major air pollutants affecting the daily number of clinic visits on URTI, to which females seemed more vulnerable than males.
- Published
- 2020
17. Analysis of clinical characteristics of SARS-CoV-2 infected cases: a retrospective study of medical records
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Jian Zhang, Lian Lu, Wei Zhu, Qi-Qiang He, Wei Hu, Han Zhang, De-Jia Li, and Cheng-Cao Sun
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medicine.medical_specialty ,business.industry ,Medical record ,Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) ,Emergency medicine ,Medicine ,Retrospective cohort study ,business - Abstract
Objectives In order to identify the clinical characteristics of patients with Corona Virus Disease 2019 (COVID-19) and find out the characteristic effects of 2019 New Coronavirus (SARS-CoV-2) infection on changes in clinical and laboratory data. Patients and methods From January 22 to February 13, 2020, we enrolled consecutive patients with acute respiratory tract symptoms admitted to a hospital (Ezhou, Hubei, China). For different data types t-test, the variables associated with the diagnosis of COVID-19 were compared by chi-square test and u-test, the statistically significant variables (P-valueResults 62 (77.5%) confirmed cases and 18 (22.5%) negative cases were confirmed by SARS-CoV-2 nucleic acid test. Epidemiological investigation and statistical analysis were carried out on the clinical and laboratory data of all suspected cases of COVID-19, the specific indicators were found, and the clinical characteristics of COVID-19 were described. Compared with the patients with negative nucleic acid test, the patients with positive nucleic acid test showed shorter time of onset of symptoms, higher plasma CO2 level, lower eosinophil ratio, lower platelet count and hematocrit, lower serum sodium level, higher serum creatinine, higher blood urea and plasma albumin levels (all PConclusions we argue that the SARS-CoV-2 infection can cause multiple organ damage to the heart, liver, kidney and bone marrow other than lung injury.
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- 2020
18. Additional file 1 of FOXC1-mediated LINC00301 facilitates tumor progression and triggers an immune-suppressing microenvironment in non-small cell lung cancer by regulating the HIF1α pathway
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Sun, Cheng-Cao, Zhu, Wei, Li, Shu-Jun, Hu, Wei, Zhang, Jian, Zhuo, Yue, Zhang, Han, Wang, Juan, Zhang, Yu, Huang, Shao-Xin, He, Qi-Qiang, and De-Jia Li
- Abstract
Additional file 1. Supplementary Tables S1-S5 and Figs. S1-S13.
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- 2020
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19. FOXC1-mediated LINC00301 facilitates tumor progression and triggers an immune-suppressing microenvironment in non-small cell lung cancer by regulating the HIF1α pathway
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Cheng-Cao Sun, Wei Zhu, Shu-Jun Li, Wei Hu, Jian Zhang, Yue Zhuo, Han Zhang, Juan Wang, Yu Zhang, Shao-Xin Huang, Qi-Qiang He, and De-Jia Li
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0301 basic medicine ,Lung Neoplasms ,lcsh:QH426-470 ,Enhancer of zeste homolog 2 (EZH2) ,lcsh:Medicine ,Hypoxia-inducible factor 1 α (HIF1α) ,Non-small cell lung cancer (NSCLC) ,Regulatory T cells (Treg) ,Histones ,Immunomodulation ,03 medical and health sciences ,Mice ,0302 clinical medicine ,T-Lymphocyte Subsets ,ELL protein-associated factor 2 (EAF2) ,Carcinoma, Non-Small-Cell Lung ,Cell Line, Tumor ,Genetics ,Tumor Microenvironment ,Animals ,Humans ,Nucleotide Motifs ,Promoter Regions, Genetic ,Molecular Biology ,Genetics (clinical) ,Binding Sites ,Research ,lcsh:R ,Cell Cycle ,Correction ,Forkhead Transcription Factors ,DNA Methylation ,Hypoxia-Inducible Factor 1, alpha Subunit ,Prognosis ,Gene Expression Regulation, Neoplastic ,lcsh:Genetics ,Disease Models, Animal ,030104 developmental biology ,030220 oncology & carcinogenesis ,LINC00301 ,Tumorigenesis ,Disease Progression ,Molecular Medicine ,RNA, Long Noncoding ,miR-1276 ,Protein Binding ,Signal Transduction - Abstract
Background Long non-coding RNAs (lncRNAs) are extensively intricate in the tumorigenesis and metastasis of various cancer types. Nevertheless, the detailed molecular mechanisms of lncRNA in non-small cell lung cancer (NSCLC) still remain mainly undetermined. Methods qPCR was performed to verify LINC00301 expression in NSCLC clinical specimens or cell lines. Fluorescence in situ hybridization (FISH) was conducted to identify the localization of LINC00301 in NSCLC cells. Chromatin immunoprecipitation (ChIP) was subjected to validate the binding activity between FOXC1 and LINC00301 promoters. RNA immunoprecipitation (RIP) was performed to explore the binding activity between LINC00301 and EZH2. RNA pull-down followed by dot-blot, protein domain mapping, and RNA electrophoresis mobility shift assay (EMSA) were conducted to identify the detailed binding regions between LINC00301 and EZH2. Alpha assay was conducted to quantitatively assess the interaction between LINC00301 and EZH2. Results LINC00301 is highly expressed in NSCLC and closely corelated to its prognosis by analyzing the relationship between differentially expressed lncRNAs and prognosis in NSCLC samples. in vitro and in vivo experiments revealed that LINC00301 facilitates cell proliferation, releases NSCLC cell cycle arrest, promotes cell migration and invasion, and suppresses cell apoptosis in NSCLC. In addition, LINC00301 increases regulatory T cell (Treg) while decreases CD8+ T cell population in LA-4/SLN-205-derived tumors through targeting TGF-β. The transcription factor FOXC1 mediates LINC00301 expression in NSCLC. Bioinformatics prediction and in vitro experiments indicated that LINC00301 (83–123 nucleotide [nt]) can directly bind to the enhancer of zeste homolog 2 (EZH2) (612–727 amino acid [aa]) to promote H3K27me3 at the ELL protein-associated factor 2 (EAF2) promoter. EAF2 directly binds and stabilizes von Hippel–Lindau protein (pVHL), so downregulated EAF2 augments hypoxia-inducible factor 1 α (HIF1α) expression by regulating pVHL in NSCLC cells. Moreover, we also found that LINC00301 could function as a competing endogenous RNA (ceRNA) against miR-1276 to expedite HIF1α expression in the cytoplasm of NSCLC cells. Conclusions In summary, our present research revealed the oncogenic roles of LINC00301 in clinical specimens as well as cellular and animal experiments, illustrating the potential roles and mechanisms of the FOXC1/LINC00301/EZH2/EAF2/pVHL/HIF1α and FOXC1/LINC00301/miR-1276/HIF1α pathways, which provides novel insights and potential theraputic targets to NSCLC.
- Published
- 2019
20. Fluorescence spectra and enzymatic property of hemoglobin as mimetic peroxidase
- Author
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De-jia, Li, Hai-cheng, Li, and Guo-lin, Zou
- Published
- 2003
- Full Text
- View/download PDF
21. Study on the intermediate in theo-phenylenediamine oxidative reaction using hemoglobin as a mimetic peroxidase in aqueous-organic two phase
- Author
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Hai-cheng, Li, De-jia, Li, Bo, Huang, De-long, Jin, and Guo-lin, Zou
- Published
- 2002
- Full Text
- View/download PDF
22. RETRACTED: Comprehensive Analysis of the Expression and Prognosis for E2Fs in Human Breast Cancer
- Author
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Feng Zhang, Qian Zhang, Zhuoyue Bi, Wei Hu, Jian Zhang, Yiyan Songyang, Zhan-Peng Yuan, Cong Liu, Bo-Tao Zhan, Qi-Qiang He, Yongyi Bi, Qun Zhou, Lin-Lin Li, Shu-Jun Li, Zhen-Long Chen, Wei-Dong Hu, Guang Li, Cheng-Cao Sun, Feng-Yun Gong, De-Jia Li, and Tao Bo
- Subjects
Oncology ,medicine.medical_specialty ,Breast Neoplasms ,Kaplan-Meier Estimate ,medicine.disease_cause ,Disease-Free Survival ,03 medical and health sciences ,0302 clinical medicine ,Breast cancer ,Internal medicine ,Drug Discovery ,Genetics ,medicine ,Biomarkers, Tumor ,Humans ,RNA, Messenger ,Precision Medicine ,Molecular Biology ,E2F4 ,Survival analysis ,030304 developmental biology ,E2F2 ,Neoplasm Staging ,Pharmacology ,0303 health sciences ,business.industry ,Gene Expression Profiling ,Computational Biology ,medicine.disease ,Prognosis ,E2F Transcription Factors ,Retraction ,Gene expression profiling ,Gene Expression Regulation, Neoplastic ,Tumor progression ,030220 oncology & carcinogenesis ,Disease Progression ,Molecular Medicine ,Original Article ,Female ,Neoplasm Recurrence, Local ,Carcinogenesis ,business - Abstract
E2F transcription factors (E2Fs), a group of genes that encode a family of transcription factors, have been identified as being involved in the tumor progression of various cancer types. Increasing experimental evidence indicates that E2Fs are implicated in breast cancer tumorigenesis. However, the diverse expression patterns and prognostic values of eight E2Fs have yet to be analyzed. Herein we investigated the transcriptional and survival data of E2Fs in patients with breast cancer from the Oncomine, Gene Expression Profiling Interactive Analysis (GEPIA), Kaplan-Meier Plotter, and cBioPortal databases. We found that the expression levels of E2F1–3 and 5–8 were higher in breast cancer tissues than in normal breast tissues, whereas the expression level of E2F4 was lower in the former than in the latter. The expression levels of E2F2, 5, 7, and 8 were correlated with advanced tumor stage. Survival analysis using the Kaplan-Meier Plotter database revealed that the high transcription levels of E2F1–3, 5, 7, and 8 were associated with low relapse-free survival in all of the patients with breast cancer. Conversely, high E2F4 and E2F6 levels predicted high relapse-free survival in these patients. This study implied that E2F1–3, 5, 7, and 8 are potential targets of precision therapy for patients with breast cancer and that E2F4 and 6 are new biomarkers for the prognosis of breast cancer.
- Published
- 2019
23. Improved texture and magnetic properties in thin-gauge strip-cast non-oriented electrical steel through matching design of hot-cold rolling
- Author
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Hai-tao Jiao, Xin-xiang Xie, Yan-chuan Tang, Yong Hu, De-jia Liu, and Long-zhi Zhao
- Subjects
Texture ,Magnetic properties ,Non-oriented electrical steel ,Rolling process ,Recrystallization behavior ,Mining engineering. Metallurgy ,TN1-997 - Abstract
In this study, an optimized matching design of hot rolling and one-stage cold rolling was adopted to prepare thin-gauge strip-cast non-oriented electrical steel. The microstructure and texture evolution of the whole processing process were studied. The results indicated that the deformed microstructure after direct cold rolling was dominated by fibrous bands, and the annealed sheet exhibited strong γ-fiber and {114} texture. However, after introducing appropriate hot rolling with reduction of 40–55%, the shear bands substructure in the cold rolled sheet were effectively increased. The unfavorable γ-fiber texture in the annealed sheets was weakened, and the favorable Goss texture was significantly enhanced. In addition, compared to direct cold rolling, the combined processing of hot rolling and cold rolling reduced the dislocation density of the cold rolled microstructure, resulting in the increase in the average grain size of annealed microstructure. Moreover, the homogeneity of microstructure was improved. Finally, the magnetic properties of the thin-gauge non-oriented electrical steel were significantly improved.
- Published
- 2023
- Full Text
- View/download PDF
24. Household infection: The predominant risk factor for close contacts of patients with COVID-19
- Author
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Xiangxiang Liu, Peigang Wang, Xi-Qing Yao, Yong-feng Hu, Hong-jie Yu, De-Jia Li, Dan Yang, Qi-Fa Wang, Li-Ping Liu, and Qi-qiang He
- Subjects
2019-20 coronavirus outbreak ,Geography ,Infectious Diseases ,Coronavirus disease 2019 (COVID-19) ,Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) ,Public Health, Environmental and Occupational Health ,Risk factor ,Coronavirus Infections ,Article ,Demography - Published
- 2020
25. Maternal obesity alters circRNA expression and the potential role of mmu_circRNA_0000660 via sponging miR_693 in offspring liver at weaning age
- Author
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Jie Zhang, De-Jia Li, Qiu-tong Chen, Hong-jie Yu, Yongwen Luo, Ming-Wei Liu, and Qi-qiang He
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Male ,0301 basic medicine ,Small interfering RNA ,Offspring ,Weaning ,Biology ,Obesity, Maternal ,Andrology ,Mice ,03 medical and health sciences ,0302 clinical medicine ,Pregnancy ,Circular RNA ,microRNA ,Genetics ,Animals ,Gene ,Cells, Cultured ,Messenger RNA ,Competing endogenous RNA ,RNA ,RNA, Circular ,General Medicine ,Animals, Suckling ,Mice, Inbred C57BL ,MicroRNAs ,030104 developmental biology ,Liver ,Prenatal Exposure Delayed Effects ,030220 oncology & carcinogenesis ,Female - Abstract
Background and Aims Maternal obesity predispose offspring to metabolic disorders and obesity, but the mechanisms are not fully understood, especially during early life. Circular RNA (circRNA) can regulate the expression of target genes through the regulatory pathways of competing endogenous RNA (ceRNA). We hypothesized that the offspring of obese dams exhibit impaired metabolic health through the dysregulated expression of hepatic circRNA. Methods and Results A high-fat diet (HFD) or standard chow diet (CD) were randomized to dams for 12 weeks before mating. Specific diets continued for each dam throughout pregnancy and lactation. Then, lipid metabolic parameters were assessed in dams and female offspring. We performed liver RNA sequencing (RNA-seq) for the offspring of HFD- and CD-dams to comprehensively identify differentially expressed (DE) circRNA and messenger RNA (mRNA). Further, ceRNA networks combining DE circRNA, mRNA, and microRNA were predicted based on MiRanda and TargetScan databases combined with the lipid metabolism-related pathway. As a result, the circRNA_0000660-miR_693-Igfbp1 regulatory pathway was selected from liver and AML12 cell line. Quantitative real-time polymerase chain reaction, dual luciferase reporter gene system, and Small interfering RNA for circRNA_0000660 transfection experiment were applied to validate. Conclusions Our work investigated new mechanisms of the effect of maternal obesity on offspring’s lipid metabolism. Several novel targets were uncovered to reverse the effect.
- Published
- 2020
26. Transcriptional E2F1/2/5/8 as potential targets and transcriptional E2F3/6/7 as new biomarkers for the prognosis of human lung carcinoma
- Author
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Tao Bo, Zhan-Peng Yuan, Feng-Yun Gong, Zhen-Long Chen, Shu-Jun Li, Guang Li, De-Jia Li, Feng Zhang, Zhuoyue Bi, Qi-Zhu Tang, Cheng-Cao Sun, Yongyi Bi, Bo-Tao Zhan, Wei Hu, Qun Zhou, Wei-Dong Hu, and Qian Zhang
- Subjects
0301 basic medicine ,Aging ,Lung Neoplasms ,Kaplan-Meier Estimate ,Biology ,medicine.disease_cause ,03 medical and health sciences ,0302 clinical medicine ,E2F translational factors ,Carcinoma, Non-Small-Cell Lung ,Kaplan-Meier plot ,medicine ,Biomarkers, Tumor ,E2F1 ,Humans ,Lung cancer ,E2F ,E2F4 ,Survival analysis ,E2F2 ,Cell Biology ,medicine.disease ,Small Cell Lung Carcinoma ,030104 developmental biology ,030220 oncology & carcinogenesis ,Cancer research ,Adenocarcinoma ,prognosis ,Carcinogenesis ,lung carcinoma ,Transcription Factors ,Research Paper - Abstract
E2F is a group of genes that encode a family of transcription factors (TFs) in higher eukaryotes and participate in cell cycle regulation and DNA synthesis in mammalian cells. Evidence from cell lines, mouse models, and human tissues indicates that TFs are implicated in lung cancer (LC) tumorigenesis. However, the diverse expression patterns and prognostic values of eight E2Fs have yet to be elucidated. In the current study, we examined the transcriptional and survival data of E2Fs in patients with LC from ONCOMINE, GEPIA, Kaplan-Meier Plotter, and cBioPortal databases. We found that the expression levels of E2F1/2/3/5/6/7/8 were higher in lung adenocarcinoma and squamous cell lung carcinoma tissues than in lung tissues, whereas the expression level of E2F4 was lower in the former than in the latter. The expression levels of E2F2/4/5/7/8 were correlated with advanced tumor stage. Survival analysis using the Kaplan-Meier Plotter database revealed that the high transcription levels of E2F1/2/4/5/7/8 were associated with low relapse-free survival (RFS) in all of the patients with LC. Conversely, high E2F3/6 levels predicted high RFS in these patients. This study implied that E2F3/6/7 are potential targets of precision therapy for patients with LC and that E2F1/2/4/5/8 are new biomarkers for the prognosis of LC.
- Published
- 2018
27. Editorial: Towards MiRNA Based Therapeutics for Lung Cancer
- Author
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Cheng-Cao Sun and De-Jia Li
- Subjects
0301 basic medicine ,Pharmacology ,Lung Neoplasms ,business.industry ,Antineoplastic Agents ,medicine.disease ,03 medical and health sciences ,030104 developmental biology ,Drug Resistance, Neoplasm ,Carcinoma, Non-Small-Cell Lung ,Drug Discovery ,microRNA ,Cancer research ,Medicine ,Animals ,Humans ,business ,Lung cancer - Published
- 2018
28. Emerging landscape of circular RNAs in lung cancer
- Author
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Qian Zhang, Wei Zhu, De-Jia Li, Yongyi Bi, Cheng-Cao Sun, Cong Liu, Yang-Yi-Yan Song, Lin-Lin Li, Feng Zhang, Qun Zhou, Zhen-Long Chen, Wei Hu, Zhuoyue Bi, and Bo-Tao Zhan
- Subjects
0301 basic medicine ,Cancer Research ,Lung Neoplasms ,RNA, Untranslated ,Treatment of lung cancer ,Computational biology ,Disease ,Biology ,03 medical and health sciences ,Circular RNA ,medicine ,RNA Precursors ,Malignant cells ,Humans ,Lung cancer ,Models, Genetic ,RNA ,Cancer ,High-Throughput Nucleotide Sequencing ,Exons ,RNA, Circular ,respiratory system ,medicine.disease ,Biomarker (cell) ,Alternative Splicing ,MicroRNAs ,030104 developmental biology ,Oncology - Abstract
Lung cancer, the leading cause of cancer deaths worldwide, is characterized with malignant cell growth. Advances in next-generation sequencing has helped us further understand RNA and identify novel circular RNAs (circRNAs) that may be useful in the early diagnosis and treatment of lung cancer. Similar to other noncoding RNAs, circRNAs present diverse biological functions in normal and disease states, including various types of cancers. This review focuses mainly on the poorly understood functions of circRNA in lung cancer. This paper also summarizes the recent advances in circRNA biogenesis, analyzes the role of circRNAs in cancers, and discusses the potential mechanisms of circRNAs in lung cancer.
- Published
- 2017
29. A novel fluorescent method for determination of peroxynitrite using folic acid as a probe
- Author
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Jie Hou, Jun-Chao Huang, De-Jia Li, Junchen Diao, Jiang-Lan Yuan, and Guo-Lin Zou
- Subjects
Detection limit ,Chromatography ,Chemistry ,Metal ions in aqueous solution ,Analytical chemistry ,Fluorescence spectrometry ,Fluorescence ,Fluorescence spectroscopy ,Analytical Chemistry ,Metal ,chemistry.chemical_compound ,Folic acid ,visual_art ,visual_art.visual_art_medium ,Peroxynitrite - Abstract
A novel method for the determination of peroxynitrite using folic acid as a fluorescent probe is described. The method is based on the oxidation of the reduced, low-fluorescent folic acid by peroxynitrite to produce a high-fluorescent emission product. The fluorescence increase is linearly related to the concentration of peroxynitrite in the range of 3x10(-8) to 5.0x10(-6)molL(-1) with a correlation coefficient of 0.998, and the detection limit is 1x10(-8)molL(-1). Interferences from some metal ions normally seen in biological samples, and also some anions structurally similar to peroxynitrite were studied. The optimal conditions for the detection of peroxynitrite were evaluated.
- Published
- 2007
30. The role of sulforaphane on duchenne muscular dystrophy by activation of Nrf2
- Author
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Jing-Yu Pan, Shu-Jun Li, Cheng-Cao Sun, and De-Jia Li
- Subjects
musculoskeletal diseases ,business.industry ,Duchenne muscular dystrophy ,Muscle inflammation ,Molecular pathway ,medicine.disease ,Bioinformatics ,medicine.disease_cause ,chemistry.chemical_compound ,chemistry ,medicine ,Cancer research ,Muscular dystrophy ,Signal transduction ,business ,Oxidative stress ,Sulforaphane - Abstract
Sulforaphane (SFN) possesses powerful chemo-preventive effects and plays a crucial role on oxidative stress and inflammatory. In our recent study, SFN treatment could relieve muscular dystrophy in mdx mice by activating Nrf2 (NF-E2 related factor 2). Moreover, our findings indicated that SFN-activated Nrf2 alleviated muscle inflammation in dystrophin-deficient mdx mice through suppressing NF-κB signaling pathway. Collectively, SFNinduced Nrf2 molecular pathway might be a promising approach for treatment of the patients with Duchenne muscular dystrophy.
- Published
- 2015
31. Reactions of Peroxynitrite and Nitrite with Organic Molecules and Hemoglobin
- Author
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De-jia Li, Guolin Zou, Hua Luo, and Run-wei Yan
- Subjects
inorganic chemicals ,Phenylenediamines ,Photochemistry ,Biochemistry ,Hemoglobins ,chemistry.chemical_compound ,Phenols ,Peroxynitrous Acid ,Organic Chemicals ,Nitrite ,Hydrogen peroxide ,Nitrites ,chemistry.chemical_classification ,Chemistry ,Superoxide ,Guaiacol ,Aromatic amine ,Hydrogen Peroxide ,General Medicine ,Kinetics ,Peroxynitrous acid ,Spectrophotometry ,cardiovascular system ,Hemoglobin ,Oxidation-Reduction ,Peroxynitrite - Abstract
In this work, the reactions of nitrite (NO2-) and peroxynitrite (ONOO-) with organic molecules as well as with hemoglobin (Hb) were examined and the potential interference with the detection of hydrogen peroxide and Hb was investigated. ONOO- at low concentrations (35-140 microM) induced a concentration-dependent oxidation of o-phenylenediamine and guaiacol, and this process can be improved by the addition of Hb in a concentration-dependent manner. This enhancing effect of Hb was possibly due to the formation of such highly reactive species as ferrylHb during the reaction of ONOO- and Hb. NO2- also oxidized the aromatic amine o-phenylenediamine, but its efficiency was much lower than that of ONOO-. A 300-fold excess of NO2- over hydrogen peroxide inhibited the oxidation of Pyrogallol Red mediated by hydrogen peroxide and Hb, which was due in part to the reaction of NO2- with Hb ferryl species compound I and compound II and the phenoxyl radical. These data suggest that ONOO- and NO2- can interfere with the detection of hydrogen peroxide. The overestimation or underestimation of the hydrogen peroxide detected is dependent upon the organic molecule utilized for detection and the relative rate of NO2-, superoxide, and ONOO- generation.
- Published
- 2005
32. Neutral porphyrin J-aggregates in premicellar SDS solution
- Author
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De-jia Li, Xiwen Li, Mingyue Han, Guolin Zou, and Zhangping Chen
- Subjects
Aqueous solution ,Inorganic chemistry ,Photochemistry ,Porphyrin ,Fluorescence ,Light scattering ,chemistry.chemical_compound ,Delocalized electron ,Colloid and Surface Chemistry ,chemistry ,polycyclic compounds ,Sodium dodecyl sulfate ,Absorption (chemistry) ,J-aggregate - Abstract
In aqueous neutral sodium dodecyl sulfate (SDS) solution, as meso-tetra(4-hydroxyphenyl)porphyrin (THPP) monomers insert SDS aggregation, a strong porphyrin–porphyrin interaction is observed, and then the porphyrin J-aggregates form slowly. The concentration of SDS necessary to give the maximum amount of J-aggregates is 5.0 mM. The J-aggregates have been studied by optical absorption, fluorescence, and resonance light scattering spectroscopies. The J-aggregates have two strong exciton bands corresponding to the B-band and Q-bands of THPP monomers. The total fluorescence of THPP is quenched due to the aggregate formation. A strong and sharply peaked resonance light scattering signal (at 490 nm) which suggests a delocalized excitonic state is observed just slightly to the red of the J-aggregates absorption maximum. The J-aggregates are still very steady even if the solutions were stored in the dark for more than a year.
- Published
- 2005
33. Identification of intermediate and product from methemoglobin-catalyzed oxidation of o-phenylenediamine in two-phase aqueous?organic system
- Author
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Guolin Zou, Xiwen Li, De-jia Li, Xiao-Qiang Cai, and Yu-Xiang Xie
- Subjects
Reaction mechanism ,Biophysics ,Reaction intermediate ,Phenylenediamines ,Photochemistry ,Biochemistry, Genetics and Molecular Biology (miscellaneous) ,Biochemistry ,Redox ,Catalysis ,Mass Spectrometry ,Methemoglobin ,chemistry.chemical_compound ,o-Phenylenediamine ,Aqueous solution ,biology ,Temperature ,Hydrogen Peroxide ,General Medicine ,Oxidants ,Models, Chemical ,chemistry ,Spectrophotometry ,Solvents ,biology.protein ,Phenazines ,Geriatrics and Gerontology ,Oxidation-Reduction ,Peroxidase - Abstract
Methemoglobin (metHb) was used as a mimetic enzyme for peroxidase to catalyze the oxidation reaction of o-phenylenediamine (OPDA) with H2O2 functioning as an oxidant. A reaction intermediate was obtained in two-phase aqueous-organic system and an absorption peak at 710 nm was confirmed to be that of the intermediate in relation to OPDA. The isolated product and intermediate were characterized by UV-V is and IR spectrophotometry and HPLC-tandem mass spectrometry. The results showed that the product is 2,3-diaminophenazine, the molecular mass of the intermediate is 212 daltons, and a conceivable structure of the intermediate is suggested. Combining the catalyzed reaction mechanism of peroxidase and our experimental results, a conceivable oxidation reaction mechanism of OPDA and H2O2 using metHb as catalyst is proposed.
- Published
- 2005
34. Spectrophotometric Determination of Peroxynitrite Usingo‐Phenylenediamine as a Probe
- Author
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Xuan Zeng, De‐Jia Li, Guo‐Lin Zou, and Li‐Lin Wang
- Subjects
Detection limit ,medicine.diagnostic_test ,Biochemistry (medical) ,Clinical Biochemistry ,Inorganic chemistry ,Photochemistry ,Biochemistry ,Analytical Chemistry ,Absorbance ,chemistry.chemical_compound ,chemistry ,Spectrophotometry ,o-Phenylenediamine ,Electrochemistry ,medicine ,Reaction system ,Spectroscopy ,Peroxynitrite - Abstract
In the present study, a novel spectrophotometric method for the determination of peroxynitrite using, o‐phenylenediamine as a probe is described. The method is based on the oxidation of o‐phenylenediamine, a colorless substance, by peroxynitrite to give a colored product. The absorbance increase of the reaction system is linearly related to the concentration of peroxynitrite in the range of 4.4 × 10−7–8.0 × 10−6 mol l−1 with a detection limit of 1.7 × 10−7 mol l−1 (3σ). The optimal conditions for the detection of peroxynitrite were evaluated and the possible detection mechanism was also discussed in this paper.
- Published
- 2004
35. Echinocystic acid ameliorates hyperhomocysteinemia-induced vascular endothelial cell injury through regulating NF-κB and CYP1A1.
- Author
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CHUAN-FENG HUANG, WEI-NA WANG, CHENG-CAO SUN, YU-QING WANG, LING LI, YIN LI, and DE-JIA LI
- Subjects
NF-kappa B ,CYTOCHROME P-450 ,HYPERHOMOCYSTEINEMIA ,VITAMIN B2 ,SPRAGUE Dawley rats - Abstract
The present study investigated the role of echinocystic acid (EA) on the expression of nuclear factor (NF)-κB and cytochrome P450 1A1 (CYP1A1), and aortic morphology, in a rat model of hyperhomocysteinemia (Hhcy). A total of 50 Sprague Dawley rats were randomly divided into five groups as follows: Normal control (NC), model control (MC), vitamin control (VC; folic acid 1 mg/kg + vitamin B2 2 mg/kg + vitamin B12 10u g/kg), EA1 (20 mg/kg EA) and EA2 (40 mg/kg EA). Plasma homocysteine (Hcy) levels were determined via high performance liquid chromatography, and the morphology of the aorta was investigated using hematoxylin and eosin staining. Furthermore, aortic mRNA and protein levels of NF-κB and CYP1A1 were measured using reverse transcription-quantitative polymerase chain reaction analysis and western blotting, respectively. Plasma Hcy levels, and aortic mRNA and protein levels of NF-κB and CYP1A1, were significantly lower in the EA-treated group compared with the MC group (all P<0.05). However, the aortic morphology remained normal, including the endothelial cells of the inner layer, and smooth muscle cells of the media layer and adventitia. In conclusion, the results of the present study indicate that EA has a protective role on vascular endothelial cells in Hhcy through decreasing plasma Hcy, and thus NF-κB and CYP1A1 expression. [ABSTRACT FROM AUTHOR]
- Published
- 2017
- Full Text
- View/download PDF
36. Potential of peroxynitrite to promote the conversion of oxyhemoglobin to methemoglobin
- Author
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Hua Luo, De-Jia Li, Guo-Lin Zou, and Li-Ling Wang
- Subjects
inorganic chemicals ,Time Factors ,Ultraviolet Rays ,Biophysics ,Heme ,Photochemistry ,Biochemistry ,Methemoglobin ,chemistry.chemical_compound ,Hemoglobins ,1-Butanol ,Superoxides ,Peroxynitrous Acid ,Oxidizing agent ,Humans ,Incubation ,Dose-Response Relationship, Drug ,Superoxide ,Temperature ,General Medicine ,Carbon Dioxide ,Hydrogen-Ion Concentration ,Lower temperature ,Oxygen ,Oxidative Stress ,chemistry ,Oxyhemoglobins ,cardiovascular system ,Hemoglobin ,Peroxynitrite - Abstract
Superoxide anion and NO can react to form the highly oxidizing species peroxynitrite (ONOO − ) which can react directly with hemoglobin (Hb) even in the presence of physiological concentration CO 2 . This research was to determine the ONOO − -mediated oxidation damage to the heme of oxyhemoglobin (oxyHb) under conditions expected in blood. Results showed that 8-10 mol ONOO − was needed to quickly and completely convert 1 mol oxyHb to methemoglobin (metHb). ONOO − (20-140 μM) caused rapid and extensive formation of metHb from oxyHb (50 μM) mainly occurring within first 5-20 min of incubation. The conversion efficiency reached 16%, 48%, 60%, 79% and 88% output of metHb after 90 min of incubation at 0, 20, 40, 100, and 140 μM ONOO − respectively. 1 mM CO 2 caused a small decrease in the ability of ONOO − to oxidize oxyHb, and ONOO − -promoted conversion of oxyHb to metHb increased when pH decreased from 8.0 to 6.0. Relatively lower temperature in blood condition will inhibit this reaction in some degree. We postulate that ONOO − can mediate oxidation damage to the heme, and cause heme loss from the hydrophobic cavity of Hb when its concentration exceeded 90 μM. These results indicated that ONOO − could convert oxyHb to metHb under the conditions expected in blood, and this reaction was regulated by CO 2 concentration, reaction time, temperature and pH value.
- Published
- 2004
37. Identification of intermediate and product from methemoglobin-catalyzed oxidation ofo-phenylenediamine in two-phase aqueous-organic system.
- Author
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De-Jia Li, Xi-Wen Li, Yu-Xiang Xie, Xiao-Qiang Cai, and Guo-Lin Zou
- Subjects
- *
PHENYLENEDIAMINES , *OXIDATION , *CATALYSIS , *PEROXIDASE , *MASS spectrometry , *HIGH performance liquid chromatography - Abstract
Methemoglobin (metHb) was used as a mimetic enzyme for peroxidase to catalyze the oxidation reaction ofo-phenylenediamine (OPDA) with H2O2 functioning as an oxidant. A reaction intermediate was obtained in two-phase aqueous-organic system and an absorption peak at 710 nm was confirmed to be that of the intermediate in relation to OPDA. The isolated product and intermediate were characterized by UV-V is and IR spectrophotometry and HPLC-tandem mass spectrometry. The results showed that the product is 2,3-diaminophenazine, the molecular mass of the intermediate is 212 daltons, and a conceivable structure of the intermediate is suggested. Combining the catalyzed reaction mechanism of peroxidase and our experimental results, a conceivable oxidation reaction mechanism of OPDA and H2O2 using metHb as catalyst is proposed. [ABSTRACT FROM AUTHOR]
- Published
- 2005
- Full Text
- View/download PDF
38. Study on the intermediate in the o-phenylenediamine oxidative reaction using hemoglobin as a mimetic peroxidase in aqueous-organic two phase.
- Author
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Hai-cheng, Li, De-jia, Li, Bo, Huang, De-long, Jin, and Guo-lin, Zou
- Abstract
Hemoglobin was used as a mimetic enzyme for peroxidase to catalyze the oxidative reaction of o-phenylenediamine with H
2 O2 which functioned as an oxidant. The relationship between physicochemical properties of the intermediate and enzymatic activity of hemoglobin was studied. Since the solubility of the intermediate in the reaction is higher in butanol phase than in water phase, the intermediate itself diffused from the aqueous phase to the butanol phase. The experimental results showed that the rate of product and the stability of intermediate were associated with the temperature and the pH value of the buffer. The formation rate of intermediate and half-life period reveal the maximal in pH7, nevertheless, the whole rate of the catalytic reaction is the greatest in pH5, which the ratio of the initial rate in final product formation compared to that intermediate formation is the greatest. [ABSTRACT FROM AUTHOR]- Published
- 2002
- Full Text
- View/download PDF
39. Sulforaphane Attenuates Muscle Inflammation in Dystrophin-deficient mdx Mice via NF-E2-related Factor 2 (Nrf2)-mediated Inhibition of NF-κB Signaling Pathway.
- Author
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Cheng-Cao Sun, Shu-Jun Li, Cui-Li Yang, Rui-Lin Xue, Yong-Yong Xi, Liang Wang, Qian-Long Zhao, and De-Jia Li
- Subjects
- *
DUCHENNE muscular dystrophy , *INFLAMMATION , *MUSCLE diseases , *OXIDATIVE stress , *LABORATORY mice , *SULFORAPHANE , *THERAPEUTICS - Abstract
Inflammation is widely distributed in patients with Duchenne muscular dystrophy and ultimately leads to progressive deterioration of muscle function with chronic muscle damage, oxidative stress, and reduced oxidative capacity. NF-E2-related factor 2 (Nrf2) plays a critical role in defending against inflammation in different tissues via activation of phase II enzyme heme oxygenase-1 and inhibition of the NF-κB signaling pathway. However, the role of Nrf2 in the inflammation of dystrophic muscle remains unknown. To determine whether Nrf2 may counteract inflammation in dystrophic muscle, we treated 4-week-old male mdx mice with the Nrf2 activator sulforaphane (SFN) by gavage (2 mg/kg of body weight/day) for 4 weeks. The experimental results demonstrated that SFN treatment increased the expression of muscle phase II enzyme heme oxygenase-1 in an Nrf2-dependent manner. Inflammation in mice was reduced by SFN treatment as indicated by decreased infiltration of immune cells and expression of the inflammatory cytokine CD45 and proinflammatory cytokines tumor necrosis factor-α, interleukin-1β, and interleukin-6 in the skeletal muscles of mdx mice. In addition, SFN treatment also decreased the expression of NF-κB(p65) and phosphorylated IκB kinase-α as well as increased inhibitor of κB-α expression in mdx mice in an Nrf2-dependent manner. Collectively, these results show that SFN-induced Nrf2 can alleviate muscle inflammation in mdx mice by inhibiting the NF-κB signaling pathway. [ABSTRACT FROM AUTHOR]
- Published
- 2015
- Full Text
- View/download PDF
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