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1. Two tomato GDP-D-mannose epimerase isoforms involve in ascorbate biosynthesis play specific roles in cell wall biosynthesis and development

Author

Kentaro Mori, Jean-Claude Mollet, Christophe Rothan, Michel Hernould, Louise Mounet-Gilbert, Pierre Baldet, Joana Jorly, Martine Lemaire-Chamley, Carine Ferrand, Céline Bournonville, Arnaud Lehner, Marie Dumont, Antoine Monier, Isabelle Atienza, Patrice Lerouge, Rebecca Stevens, Biologie du fruit et pathologie (BFP), Université Sciences et Technologies - Bordeaux 1-Université Bordeaux Segalen - Bordeaux 2-Institut National de la Recherche Agronomique (INRA), Laboratoire de Glycobiologie et Matrice Extracellulaire Végétale (Glyco-MEV), Université de Rouen Normandie (UNIROUEN), Normandie Université (NU)-Normandie Université (NU), Génétique et Amélioration des Fruits et Légumes (GAFL), Institut National de la Recherche Agronomique (INRA), Université Bordeaux Segalen - Bordeaux 2-Institut National de la Recherche Agronomique (INRA)-Université Sciences et Technologies - Bordeaux 1, Unité de recherche Génétique et amélioration des fruits et légumes (GALF), and Normandie Université (NU)

Subjects
0106 biological sciences, 0301 basic medicine, tomato fruits, Physiology, [SDV]Life Sciences [q-bio], Mannose, Plant Science, Ascorbic Acid, 01 natural sciences, chemistry.chemical_compound, tomate, Gene Expression Regulation, Plant, ascorbate, cell wall, GDP-D-mannose epimerase, growth, rhamnogalacturonan-II, solanum lycopersicum, 5-epimerase, chemistry.chemical_classification, Vegetal Biology, rhamnogalacturonane, food and beverages, PMI: phosphomannose isomerase, GMP: GDP-D-mannose pyrophosphorylase, [SDV.BV.BOT]Life Sciences [q-bio]/Vegetal Biology/Botanics, [SDV.BBM.BC]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Biomolecules [q-bio.BM], Agricultural sciences, Isoenzymes, tomato fruits AsA: ascorbic acid, Biochemistry, développement du fruit, Pollen, paroi cellulaire végétale, Research Paper, Gene isoform, Germination, AsA: ascorbic acid, Biology, Cell wall, 03 medical and health sciences, Biosynthesis, gdp mannose épimérase, [SDV.BBM.BC]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Biochemistry [q-bio.BM], Gene, DPA: days post anthesis, fungi, biosynthèse de la paroi cellulaire, Metabolism, 030104 developmental biology, Enzyme, chemistry, Carbohydrate Epimerases, Function (biology), GME: GDP-D-mannose-3, Biologie végétale, Sciences agricoles, 010606 plant biology & botany
Abstract

Highlight The two tomato GDP-D-mannose epimerase isoforms play specific roles in cell wall biosynthesis and plant development but participate similarly in ascorbate biosynthesis., GDP-D-mannose epimerase (GME, EC 5.1.3.18) converts GDP-D-mannose to GDP-L-galactose, and is considered to be a central enzyme connecting the major ascorbate biosynthesis pathway to primary cell wall metabolism in higher plants. Our previous work demonstrated that GME is crucial for both ascorbate and cell wall biosynthesis in tomato. The aim of the present study was to investigate the respective role in ascorbate and cell wall biosynthesis of the two SlGME genes present in tomato by targeting each of them through an RNAi-silencing approach. Taken individually SlGME1 and SlGME2 allowed normal ascorbate accumulation in the leaf and fruits, thus suggesting the same function regarding ascorbate. However, SlGME1 and SlGME2 were shown to play distinct roles in cell wall biosynthesis, depending on the tissue considered. The RNAi-SlGME1 plants harbored small and poorly seeded fruits resulting from alterations of pollen development and of pollination process. In contrast, the RNAi-SlGME2 plants exhibited vegetative growth delay while fruits remained unaffected. Analysis of SlGME1- and SlGME2-silenced seeds and seedlings further showed that the dimerization state of pectin rhamnogalacturonan-II (RG-II) was altered only in the RNAi-SlGME2 lines. Taken together with the preferential expression of each SlGME gene in different tomato tissues, these results suggest sub-functionalization of SlGME1 and SlGME2 and their specialization for cell wall biosynthesis in specific tomato tissues.

Published
2016
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2. A diminution in ascorbate oxidase activity affects carbon allocation and improves yield in tomato under water deficit

Author

Garchery, Cécile, Gest, Noé, Do, Phuc Thi, Alhagdow, Moftah, Baldet, Pierre, Menard, Guillaume, Rothan, Christophe, Massot, Capucine, Gautier, Hélène, Aarrouf, Jawad, Fernie, Alisdair R., Stevens, Rebecca, Génétique et Amélioration des Fruits et Légumes (GAFL), Institut National de la Recherche Agronomique (INRA), Max Planck Institute of Molecular Plant Physiology (MPI-MP), Max-Planck-Gesellschaft, Station de physiologie végétale, Biologie du fruit et pathologie (BFP), Université Sciences et Technologies - Bordeaux 1-Université Bordeaux Segalen - Bordeaux 2-Institut National de la Recherche Agronomique (INRA), Unité de recherche Plantes et Systèmes de Culture Horticoles (PSH), EA 4279 Laboratoire Physiologie des Fruits et Légumes, Avignon Université (AU), INRA, Région Provence-Alpes-Côte d'Azur, Faculty of Biology, Vietnam National University, Université Bordeaux Segalen - Bordeaux 2-Institut National de la Recherche Agronomique (INRA)-Université Sciences et Technologies - Bordeaux 1, Institut des Nanosciences de Paris (INSP), Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS), Laboratoire d'Informatique, Signaux, et Systèmes de Sophia-Antipolis (I3S) / Equipe BIOINFO, Modèles Discrets pour les Systèmes Complexes (Laboratoire I3S - MDSC), Laboratoire d'Informatique, Signaux, et Systèmes de Sophia Antipolis (I3S), Université Nice Sophia Antipolis (... - 2019) (UNS), COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-Centre National de la Recherche Scientifique (CNRS)-Université Côte d'Azur (UCA)-Université Nice Sophia Antipolis (... - 2019) (UNS), COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-Centre National de la Recherche Scientifique (CNRS)-Université Côte d'Azur (UCA)-Laboratoire d'Informatique, Signaux, et Systèmes de Sophia Antipolis (I3S), COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-Centre National de la Recherche Scientifique (CNRS)-Université Côte d'Azur (UCA), Unité de recherche Génétique et amélioration des fruits et légumes (GALF), Université Côte d'Azur (UCA)-Université Côte d'Azur (UCA)-Centre National de la Recherche Scientifique (CNRS)-Université Nice Sophia Antipolis (... - 2019) (UNS), Université Côte d'Azur (UCA)-Université Côte d'Azur (UCA)-Centre National de la Recherche Scientifique (CNRS)-Laboratoire d'Informatique, Signaux, et Systèmes de Sophia Antipolis (I3S), Université Côte d'Azur (UCA)-Université Côte d'Azur (UCA)-Centre National de la Recherche Scientifique (CNRS), Université Bordeaux Segalen - Bordeaux 2-Institut National de la Recherche Agronomique (INRA)-Université Sciences et Technologies - Bordeaux 1 (UB), Université Nice Sophia Antipolis (1965 - 2019) (UNS), and COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-Centre National de la Recherche Scientifique (CNRS)-Université Côte d'Azur (UCA)-Université Nice Sophia Antipolis (1965 - 2019) (UNS)

Subjects
Sucrose, Complex systems, VTC: Vitamin C, [SDV]Life Sciences [q-bio], vitamin C, Ascorbic Acid, adaptation, drought, AsA: Ascorbic acid, Key-words: Solanum lycopersicum, Biologie intégrative, MDHAR, Solanum lycopersicum, AO: Ascorbate oxidase, 5-epimerase, oxidative stress, Biomass, Monodehydroascorbate reductase Ascorbic acid, Cell wall, food and beverages, PMI: phosphomannose isomerase, GMP: GDP-D-mannose pyrophosphorylase, Paroi, rhamnogalacturonan-II, tomato fruits AsA: ascorbic acid, GME: GDP-d-mannose-3, redox, Metabolome, Carbohydrate Metabolism, RNA Interference, Oxidation-Reduction, GalLDH: L-Galactono-1, growth, water, Tomato, Systèmes complexes, diversity, Tomate, [SDV.BV]Life Sciences [q-bio]/Vegetal Biology, Ascorbate, Hexoses, 4-Lactone Dehydrogenase, Systems biology Ascorbate, GDP-D-mannose epimerase, DPA: days post anthesis, fungi, source–sink, apoplast, Plant Leaves, 5 -epimerase, Fruit, source-sink, RNAi, Plant Stomata, Ascorbate Oxidase
Abstract

International audience; The regulation of carbon allocation between photosyn-thetic source leaves and sink tissues in response to stress is an important factor controlling plant yield. Ascorbate oxidase is an apoplastic enzyme, which controls the redox state of the apoplastic ascorbate pool. RNA interference was used to decrease ascorbate oxidase activity in tomato (Solanum lycopersicum L.). Fruit yield was increased in these lines under three conditions where assimilate became limiting for wild-type plants: when fruit trusses were left unpruned, when leaves were removed or when water supply was limited. Several alterations in the transgenic lines could contribute to the improved yield and favour transport of assimilate from leaves to fruits in the ascorbate oxidase lines. Ascorbate oxidase plants showed increases in sto-matal conductance and leaf and fruit sugar content, as well as an altered apoplastic hexose : sucrose ratio. Modifications in gene expression, enzyme activity and the fruit metabolome were coherent with the notion of the ascorbate oxidase RNAi lines showing altered sink strength. Ascor-bate oxidase may therefore be a target for strategies aimed at improving water productivity in crop species.

Published
2013
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