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BACKGROUND Since the outbreak of coronavirus disease 2019 (COVID-19) in Wuhan, China in December 2019, the overall fatality rate of severe and critical patients with COVID-19 is high and the effective therapy is limited. CASE SUMMARY In this case report, we describe a case of the successful combination of the prone position (PP) and high-flow nasal oxygen (HFNO) therapy in a spontaneously breathing, severe COVID-19 patient who presented with fever, fatigue and hypoxemia and was diagnosed by positive throat swab COVID-19 RNA testing. The therapy significantly improved the patient's clinical symptoms, oxygenation status, and radiological characteristics of lung injury during hospitalization, and the patient showed good tolerance and avoided intubation. Additionally, we did not find that medical staff wearing optimal airborne personal protective equipment (PPE) were infected by the new coronavirus in our institution. CONCLUSION We conclude that the combination of PP and HFNO could benefit spontaneously breathing, severe COVID-19 patients. The therapy does not increase risk of healthcare workers wearing optimal airborne PPE to become infected with virus particles.

Depression is one of the most common psychiatric diseases in the 21st century, while its pathogenesis is not yet fully understood. Currently, besides to the monoaminergic system, the brain-derived neurotrophic factor (BDNF)-cAMP response element-binding protein (CREB) signaling is one of the most attractive signaling pathways for treating depression. Mitogen and stress-activated kinase (MSK) 1 and 2 are nuclear proteins activated downstream of the ERK1/2 or p38 MAPK pathways, and it has been demonstrated that MSKs are involved in the BDNF-CREB signaling. Here we assumed that MSKs may play a role in depression, and various methods including the chronic social defeat stress (CSDS) model of depression, western blotting, immunofluorescence and virus-mediated gene transfer were used together. It was found that CSDS fully enhanced the expression of both phosphorylated MSK1 and total MSK1 in the hippocampus but not the medial prefrontal cortex (mPFC). CSDS did not influence the expression of phosphorylated MSK2 and total MSK2 in the two brain regions. Genetic over-expression of hippocampal MSK1 fully prevented not only the CSDS-induced depressive-like behaviors but also the CSDS-induced dysfunction in the hippocampal BDNF-CREB signaling and neurogenesis in mice, while genetic knockdown of hippocampal MSK1 aggravated the CSDS-induced depressive-like symptomatology in mice. Our results collectively suggest that although CSDS evidently enhances the activity of hippocampal MSK1, it is not a contributor to the CSDS-induced dysfunction in the brain but a defensive feedback regulator which protects against CSDS. Therefore, hippocampal MSK1 participates in the pathogenesis of depression and is a feasible and potential antidepressant target.

As a highly prevalent neuropsychiatric disorder worldwide, the pathophysiology of depression is not yet fully understood and based on multiple factors among which chronic stress is critical. Numerous previous studies have shown the role of central mammalian target of rapamycin complex 1 (mTORC1) signaling in depression. However, so far it remains elusive by which way chronic stress down-regulates the activity of central mTORC1. Liver kinase b1 (LKB1) has been demonstrated to regulate the activity of the mTORC1 signaling cascade by phosphorylating AMP activated protein kinase (AMPK). Here, this study aimed to explore whether LKB1 participates in depression by regulating the downstream AMPK-mTORC1 signaling, and various methods including mouse models of depression, western blotting and immunofluorescence were used together. Our results showed that chronic stress significantly enhanced the expression of both phosphorylated LKB1 and total LKB1 in the medial prefrontal cortex (mPFC) but not the hippocampus. Furthermore, genetic knockdown of LKB1 in the mPFC fully reversed not only the depressive-like behaviors induced by chronic stress in mice but also the effects of chronic stress on the activity of AMPK and the mTORC1 system. Taken together, this study preliminarily suggests that LKB1 in the mPFC could be a feasible target for antidepressants. This study also provides support for the potential use of LKB1 inhibition strategies against the chronic stress-related neuropsychiatric disorders.

Hard carbons attract myriad interest as anode materials for high-energy rechargeable batteries due to their low costs and high theoretical capacities; practically, they deliver unsatisfactory performance due to their intrinsically disordered microarchitecture. Here we report a facile ion-catalyzed synthesis of a phenol–formaldehyde resin-based hard-carbon aerogel that takes advantage of the chelation effect of phenol and Fe3+, which consists of a three-dimensionally interconnected carbon network embedded with hydrogen-rich, ordered microstructures of expanded nanographites and carbon micropores. The chelation effect ensures the homodispersion of Fe in the polymer segments of the precursor, so that an effective catalytic conversion from sp3 to sp2 carbon occurs, enabling free rearrangement of graphene sheets into expanded nanographite and carbon micropores. The structural merits of the carbon offer chances to achieve lithium/sodium storage performance far beyond that possible with the conventional carbon a...

The practical application of lithium-sulfur batteries is usually hindered by the unstable discharge intermediates and the low conductivity of the sulfur cathode. Herein, we propose to improve the performance of the sulfur cathode by introducing a built-in carbon nanotube network inside a biomass-derived hierarchically porous carbon for the subsequent sulfur loading. This balanced structural design of the carbon substrate enables effective trapping of active sulfur within the cathode and provides a highly efficient pathway for electron transmission, contributing to an increased conductivity and structural integrity of the cathode. By using a potentiostatic process at the end of charge, the reversibility of the electrode reaction of sulfur is improved. The sulfur cathode delivers an initial discharge capacity of 1739 mA h g−1 and reaches a high initial Columbic efficiency of 87.6 %, and maintains its efficiency to >97.5 % from the 2nd cycle. It also shows admirable cycling and rate performance.

Inspired by the egg-like yolk–shell structure, a novel kind of graphene-wrapped graphitic hollow carbon spheres (G-graphitic HCS) was designed and fabricated by iron-catalyzed carbonization of double-coated polystyrene (PS) spheres. Herein, a thin carbon shell and hollow, hierarchically porous G-graphitic HCS facilitate ionic transport, while also providing more active sites for the storage of ions. Both graphitic components result in a seamless and highly efficient transmission pathway for electrons. Thus, G-graphitic HCS demonstrate admirable multifunctions in energy-storage devices, including lithium batteries, sodium batteries, and supercapacitors. It delivers an unprecedentedly high initial discharge capacity of 2007 mA h g−1 at 0.1 A g−1, and preserves 92.4 % of the initial charge capacity after 100 cycles in a prototype lithium half-cell. The present concept may shed light on designing the key materials for next-generation energy-storage devices.

Rheumatoid arthritis (RA) is an immune-mediated disease that causes chronic inflammation of the joints and involves CD4+ T cell activation. In RA, CD4+ T cells are the main drivers of disease initiation and the perpetuation of the damaging inflammatory process. In the present study, we investigated the role of Lysine-specific histone demethylase 1 (LSD1) in RA. The frequency of LSD1-positive CD4+ T cells in the synovial fluid (SF) of active RA patients was higher than that of inactive RA patients. In CD4+ T cells isolated from SF of active RA patients, LSD1 downregulation significantly increased cell proliferation, as shown by MTT assay. LSD1 knockdown also significantly increased the production of IFN-γ and IL-17, and increased that of IL-10, as determined by ELISA and qRT-PCR aasay. In CD4+ T cells isolated from SF of inactive RA patients, LSD1 was overexpressed by LSD1 plasmid transfection. As expected, LSD1 overexpression resulted in an opposite effect on cell proliferation and the production of cytokines, including IFN-γ, IL-17 and IL-10. LSD1 was downregulated in RA mouse by lenti-vector infection. As expected, LSD1 knockdown in vivo significantly alleviated the disease severity and increased the levels of anti-collagen II antibodies. LSD1 downregulation in the early stage was more effective to ameliorate disease severity. Our data suggested the potential therapeutic role of LSD1 in RA patients.

It is not clear whether TREM-2 (the "triggering receptor expressed on myeloid cells 2") is expressed in fibroblast-like synovial cells (FLSs). In this study, we aimed to determine the expression of TREM-2 in rheumatoid arthritis (RA)-FLSs and explore whether and how TREM-2 modulates the function of RA-FLSs.Western blot and RT-PCR were used to detect the expression of TREM-2 in RA-FLSs, siRNA and lentivirus were used to down-regulate and up-regulate the expression of TREM-2 in RA-FLSs. Then mRNA expression of IL-1β, IL-6, and MMP-13 was determined by RT-qPCR. Protein secretion of IL-1β, IL-6, and MMP-13 in the supernatant was determined by ELISA assay; expression of cell signal transduction molecules was determined by western blot.A: Relative to OA-FLSs, mRNA and protein expression levels of TREM-2 in RA-FLSs are significantly elevated. TREM-2 protein is mainly expressed in the cytoplasm of RA-FLSs; B: In RA, the expression of TREM-2 was reduced at first and then up-regulated after stimulation by TNF-α. TREM-2 also inhibited the activation of TNF-α induced of inflammation in RA-FLSs by the p38 pathway, which regulates the production of cytokines and matrix metalloproteinases.TREM-2 expressed in RA-FLSs and TNF-α mediated reduction of inflammatory reactions. These phenomena indicated that TREM-2 may be a potential target in the treatment of RA.

Using the TTS technology and Svox engine in the Android platform, this paper puts forward the design scheme of the speech navigation system based on Android. The Android platform and structure are introduced firstly, then the system structure and functions are detailed described, lastly the key technology and implement way is given. This system developed methods for the visually impaired application areas have certain guiding significance and the actual practical application value.

A new undergraduate's innovation and entrepreneurship education network platform system is designed by using the technology of intelligent Agent. This paper first discusses the necessity, the development system concept and basic principle of intelligent Agent technology, then analyzes the system function and system structure design, finally introduces the design of Agent system.

RFID middleware for massive data focused on the performance of server-side processing to bring pressure on the issue, a new for mobile devices can be flexibly configured RFID middleware (SMM). SMM can be used for mobile devices, has a better platform independence , can be deployed with multiple operating systems ; abstract objects by device , user via the user interface (UI) and a standard Web interface to control and manage a variety of mobile devices . Meanwhile, SMM also provides data synchronization. Experimental results show that, SMM has low energy consumption; high efficiency advantages can effectively reduce server stress and improve system performance.

The design of a multiple courses of general exam system development method based on MAS technology. This paper discussed the necessity of development system, the concept of MAS and the organizational structure of MAS, secondly analyzes the system framework, finally introduces the key technologies such as the multi-agent RPC communication model, online exam process etc..

Cloud computing is a model about the increase , usage and delivery of related services in Internet, usually provides dynamically scalable and often virtualized resources by the Internet. This paper first introduces the cloud computing technology, then discusses the system structure, system flow and system function, and finally expounds the cloud computing role and the realization of the examination system .

The paper proposed a design scheme of wireless sensor network border router based on IPv6. Project mainly expounds the realization of hardware and software design of boundary router, focuses on wireless sensor network IPv6 protocol stack adaptation layer design based on. Through the data packet fragmentation and reassembly mechanism as well as the header compression scheme, adaptation layer of protocol stack to achieve the transmission of IPv6 data packet in the IEEE in 802.15.4 link. The experimental results show that, this scheme realizes the seamless integration of wireless sensor network and IPv6 network, data transmission is reliable, practical application value.

Introduced based on RFID, GPS, GPRS technology, logistics tracking system. The system uses a combination of GPRS data transmission PC monitor management , the use of low-power control chip STM32F103RBT fusion technology GPS technology , GPRS and ARM , and other industrial areas , to achieve the objects of the logistics chain in real-time tracking , logistics links ensure that items do not lost and replaced.

In this paper, a home wireless network platform based on Zigbee technology. This article analyzes the Zigbee network protocol, discusses the key issues to build a home wireless network using the Zigbee technology. The network topology, network routing algorithms and network sampling strategies and other issues were discussed and proposed solutions by building a home wireless network monitoring experiments on the pulse signal. The results showed that based on Zigbee technology to build a home wireless network is feasible, the wireless network in the home monitoring has broad application prospects.

Tracking difficulty generally exists in the traditional warehouse management mode in the business process, the turnover, low efficiency, logistics management information processing is not seasonable and means backward, thus affecting the competitiveness of enterprises, based on the analysis of traditional logistics warehousing management deficiencies, the great advantage of application of RFID technology in logistics field to solve the above problem. The logistics management information system based on RFID is analyzed, based on the traditional logistics business processes analysis, given the information and process structure of logistics business processes, and established a framework method storage business system based on RFID technology.

UHF radio frequency identification system with write speeds, large storage capacity , recognition distance and simultaneously read multiple tags , etc. , has been more widely used in logistics. Introduced in line with ISO 18000-6 standard UHF RFID tag main characteristics, structure, working principle and write methods, proposed the corresponding reader solutions, focusing on the reader hardware design and software program flow. The application results show that the reader read and write speed (single tag 64bit / 6ms), recognition rate, recognition distance (≥ 4m).

ZigBee technology is a small disadvantage of network coverage, this article will introduce GPRS wide-area wireless communication technology ZigBee network, ZigBee network to build a remote data collection and management system, and use intuitive embedded Web server to improve management efficiency. System test results prove that the system has good validity and reliability.

This paper proposes a RFID multi criteria based on Internet of things technology set don't equipment, information RFID electronic label this equipment can automatically identify objects, has high reliability, information processing capability, low power consumption, strong confidentiality.

In this paper, we prepared the CIGS thin films with various [Cu]/[In + Ga] ratios by selenization of Cu0.8Ga0.2 and In2Se3 precursor films. The properties of Cu(In,Ga)Se2 film and related solar cell were investigated. Raman spectra confirm that the secondary Cu2−xSe phase tends to segregate at film surface. SEM results show that the grain-size improves noticeably with the increase of the [Cu]/[In + Ga] ratios. For Cu-rich CIGS films, the performances of the related solar cells were damaged greatly and good photovoltaic characteristics cannot be obtained. For near-stoichiometric and Cu-poor films, the mean conversion efficiency close to 10% was achieved over a wide range of composition.

The authors build the three-dimensional model of stack-manipulator to aluminum-ingot in SoildWorks,and then import the model into Adams to create a simulation model via the interface between them.Adams runs dynamic and kinematics simulation of the model to measure some kinematic data of manipulator and the contact force of gear part.The dynamic simulations have forecasted the performance of products based on the model well and show the importance of Adams in product development.

On the research and development of the Stack-Manipulator to Aluminum-Ingot, the Stack-Manipulator 3D model was established by using SolidWorks and imported into ADAMS via the interface between them. And then the authors got the torque and angular velocity curves after adding the driving and constraints. Finally, this paper chose the servo motors by using the simulated results. The results provide a reliable and scientific basis for the development of the control system.

This article analyzes the concept of cloud computing, the concept and difficulties of digital library, and the cloud on the Digital Library from the information resources, users, workers and facilities. Cloud computing has brought us a new perspective to look at the current resource-sharing problem, cloud computing can be applied to digital library resources to improve information sharing capabilities and improve resource utilization. Our results show that a cloud implementation may be a feasible alternative for its continued operation and growth.

In this paper, the Internet of things technology used in modern agriculture, the satellite, remote sensing, computer and automatic control, and other high and new technology applied in agricultural production, in order to improve the yield, reduce energy consumption. Of the international advanced technology and mature will be popularized to the country, with less people more agricultural development in our country, in order to solve the bottleneck, reduce pollution and waste, the road of agricultural sustainable development.

From analysis and design,it concluded that innovation was the key to promoting the development of product design.The scheme of the clean trolley functional innovative design was proposed in this paper, which includes the transmission mechanism, sweeping brush mechanism and Sprinkling mechanism.In design processing,the 3-dimensional model of clean trolley was established and the motion simulation analysis was also presented.The results showed that the presented scheme satisfy the design requirements and have the better performance.

In this paper, a novel Automatic Malware Signature Discovery System for AV cloud is proposed to generate malware signatures from both static and dynamic aspects. It keeps a good workload balance between the desktop and cloud services. It can automatically generate a lightweight signature database with the size hundreds times smaller than traditional signature ones. In the AV cloud model, users do not need to install a large virus signature file, but a lightweight set of cloud signatures. The benefits include easy deployment, low costs of operation, and fast signature updating. Further, Automatic Malware Signature Discovery System signatures can be easily integrated into existing AV products.

In view of the existing problem of the traditional aluminium ingot stacking practices, it was important significance to research and develop a stack-manipulator that includes various functions to do portage and stack. According to the demand of stacking, the motion system of the Stack-manipulator based on four degrees was finished. The kinematics equation of the manipulator was set up using the D-H theory, On this base, Some of the kinematics problems of this stack-manipulator were discussed and these reliable basis were provided for the research of the manipulators dynamics and control and trajectory planning.

In this work, we deposited LaAlO3 film on n-type GaAs by plasma enhanced atomic layer deposition (PEALD). In situ NH3 plasma nitridation of the GaAs surface was introduced to control the interface prior to plasma enhanced atomic layer deposition of LaAlO3. The chemical bonding state of NH3 plasma treated GaAs surface were investigated by X-ray photoelectron spectroscopy (XPS), which indicate that NH3 plasma nitridation can suppress interfacial growth on GaAs substrate. Transmission electron microscopy (TEM) images show the interface between LaAlO3 and GaAs was smooth and there was not obvious interface layer observed. The capacitance–voltage (C–V) and leakage current density–voltage (J–V) measurement indicate that the NH3 plasma nitridation improved the electrical properties of LaAlO3 films on GaAs substrate.

Plasma enhanced atomic layer deposition (PEALD) method can decrease film growing temperature, and allow in-situ plasma treatment. LaAlO3 films were deposited with PEALD at 180°C. High resolution transmission electron microscopy (HRTEM) results exhibited amorphous microstructure of both films even after rapid thermal annealing (RTA) at 800°C. X-ray photoelectron spectroscopy (XPS) spectra suggested that the valence-band offset between the LaAlO3 film and the substrate was 3.3 eV. The electrical experimental results indicated that the leakage current densities were 0.10mA/cm2 and 0.03mA/cm2 respectively at a gate bias of |Vg-Vfb|=1V and the equivalent oxide thicknesses (EOT) of them were 1.2 nm and 1.4 nm, respectively. The densities of interfacial states were calculated to be 1.70×1012eV-1cm-2 and 1.09×1012eV-1cm-2, respectively.

In situ NH3 plasma nitridation was utilized to passivate InP surface, HfLaOx film was grown by plasma enhanced atom layer deposition method, and the HfLaOx film remain amorphous after 500°C annealing. High-resolution transmission electron microscopy (HRTEM) images showed that in situ NH3 plasma nitridation process make the boundary between InP and HfLaOx smooth and sharp, and could suppress the formation of the interfacial layer. X-ray photoelectron spectra (XPS) results indicated In-N and P-N bonds were formed on the nitride InP surface. The electrical measurements indicated in situ NH3 plasma nitridation process reduced the hysteresis improved capacitance density and to 7 mV, a sharp transition from depletion to accumulation was observed, the interfacial density states (Dit) of the sample with nitridation was 1.67×1012 cm2 eV1, and the equivalent oxide thickness (EOT) was 0.6 nm. The leakage current was 1.5 mA/cm2 at Vg-Vfb=1V.

In this work, we present the results of an investigation into the effectiveness of varying ammonium sulphide (NH4)2S concentrations in the passivation of n-type GaAs. Samples were degreased and immersed in aqueous (NH4)2S solutions of concentrations 22% and 10%for 10 min at 295 K, immediately prior to plasma enhanced atomic layer deposition of LaAlO3. The chemical bonding state of (NH4)2S treated GaAs surface were investigated by X-ray photoelectron spectroscopy (XPS), which indicate that Sulfur passivation can reduce intrerfacial GaAs-oxide formation. Transmission electron microscopy (TEM) was implemented to characterize the interface morphology. Finally, capacitance-voltage (C-V) and leakage current density-voltage (J-V) measurement were used to characterize the electrical properties of LaAlO3 films.

In this work, La2O3 gate dielectric film was deposited by plasma enhanced atomic layer deposition. we investigate the effect of surface preparation of GaAs substrate, for example, native oxide, S-passivation, and NH3 plasma in situ treatment. The interfacial reaction mechanisms of La2O3 on GaAs is studied by means of X-ray photoelectron spectroscopy(XPS), high-resolution transmission electron microscopy(HRTEM) and atomic force microscope (AFM). As-O bonding is found to get effectively suppressed in the sample GaAs structures with both S-passivation and NH3 plasma surface treatments.

HfO2high-k dielectric films of 4nm and 5nm were both grown on Si substrate with the method of PEALD at 160°C. Both were treated with rapid thermal annealing (RTA) process at 500°C. High resolution transmission electron microscopy (HRTEM) indicated both films were not crystallized. X-ray photoelectron spectra (XPS) indicated that Hf-silicate was formed in the interfacial layer, and the valence-band offset (VBO) between the dielectric film and the substrate interface was calculated to be 3.5 eV. The electrical measurements indicated that the leakage current densities of the four and five nanometers’ sample were 1.0mA/cm2and 0.8mA/cm2at gate bias of 1V, and the equivalent oxide thicknesses of them were 0.9nm and 1.2nm respectively. Densities of interfacial states of them were calculated.

Chronic infection of Helicobacter pylori (H. pylori) in ischemic stroke (IS) incidence has been previously studied in several publications; however, conflicting results have been reported. A meta-analysis was used to assess whether chronic infection of H. pylori was associated with risk of IS, and which of the following was more effective for predication of IS risk, antibody IgG of H. pylori (anti-H. pylori IgG), antibody IgG of cytotoxin-associated gene-A (anti-Cag A IgG) or the 13C-urea breath test. We searched the databases of Medline and Embase, and latest update was January 1, 2012. Case–control studies were considered to be eligible. The odds ratio (OR) and 95 % confidence interval (95 % CI) were calculated using the random-effect model. A total of 13 studies including 4,041 participants were included in this meta-analysis. Of these studies, ten, four and four studies were for anti-H. pylori IgG, anti-Cag A IgG and the 13C-urea breath test, respectively. Combined analysis indicated that positive anti-H. pylori IgG, anti-Cag A IgG and 13C-urea breath test were significantly associated with increased risk of IS, respectively, and positive anti-Cag A IgG was more effective for predication of IS risk [OR (95 % CI) = 1.60 (1.21–2.11), P heterogeneity = 0.001 for positive versus negative anti-H. pylori IgG; 2.33 (1.76–3.09), P heterogeneity = 0.71 for positive versus negative anti-Cag A IgG and 1.65 (1.11–2.47), P heterogeneity = 0.17 for positive versus negative 13C-urea breath test]. In addition, we found that positive anti-H. pylori IgG was closely associated with risk of IS caused by atherosclerosis and small artery disease, but not for cardioembolic IS. This meta-analysis indicated that chronic H. pylori infection was significantly associated with an increased risk of IS, especially for non-cardioembolic IS. Compared with anti-H. pylori IgG and the 13C-urea breath test, anti-Cag A IgG seemed more effective for prediction of risk of IS.

The paper introduces the design objective and design scheme of mobile agent-based X.509 certificate management system, and describes the key technologies such as encryption, the certificate processing format and coding, secure communication protocols such as SSL in detaile.

To evaluate the blood flow changes and their relationships to microvessel density (MVD) and thrombospondin-1 (TSP-1) by transvaginal colour Doppler sonography (TV-CDS) in the ovarian interstitium to predict ovarian interstitial microvascular injury in the pathological process of ovarian endometrial cysts (OEC). TV-CDS was preoperatively performed to detect blood flow changes in 60 patients with 76 ovarian endometrioid cysts, and flow classification and resistance indices (RI) values were recorded for analysis. Ovarian interstitial specimens with blood flow signals were collected for postoperative pathologic examination. TSP-1 protein was evaluated by immunohistochemistry and Western blot, TSP-1 mRNA by reverse transcriptase polymerase chain reaction, microvessels by CD34 antibody, and MVD by image analysis. Thirty age-matched patients with benign ovarian tumours served as controls. Blood flow, most of star-shaped, within ovarian interstitial arteries in the OEC group was diminished; however, arterial spectra exhibited a high-resistance flow manifesting a significantly higher RI compared with that of the control group (P

The impact of the cold-side temperature and moisture to frost heave were obtained by studying frost heaving test in open system. Frost heave ratio increased linearly with the decrease of cold-side temperature and moisture. Frost heave force increased with the decrease of cold-side temperature and increase of moisture. In addition, the soil physical properties variation laws was gained during freezing in the test, like moisture and dry density. By analysising the test data of frost heave ratio and frost heave force, the relationship between frost heave ratio and frost heave force were acquired. The results had important practical value and theoretical significance to the freezing shaft sinking and artificially freezing techniques applied in urban underground engineering.

This paper introduces a design of a high-speed image acquisition system based on Avalon bus which is supported with SOPC technology. Some peripherals embedded in Avalon bus were customized and utilized in this system, such as imaging unit, decoding unit and storage unit, and these improved the speed of the whole imaging system. The data is compressed to three-fourths of the original by the decoding unit. A custom DMA is designed for moving the image data to the two caches of the SDRAM. This approach discards the method that FIFO must be put up in the traditional data acquisition system. And therefore, it reduced the CPU’s task for data moving. At the same time, the image acquisition and the data transmission can complete a parallel job. Finally, the design is worked on the high-speed image acquisition system which is made up of 2K*2K CMOS image sensor. And it improved the image acquisition speed by three ways: data encoding, custom DMA controller and the parallel processing.

In order to define clearly the conditions leading to the outcome of acute duck hepatitis B virus (DHBV) infection, 1-day-old Pekin ducklings were infected with DHBV by different routes and given different doses of inoculum. Groups of 24 ducklings were inoculated either intravenously via the vena cruralis, or intraperitoneally with pooled serum containing either 1.6 x 10(7) or 1.6 x 10(4) DHBV genomes. One control duck from each group was inoculated with an equal volume of normal duck serum. A sensitive and reproducible real-time polymerase chain reaction assay based on TaqMan technology was developed for the detection and quantitation of DHBV DNA in the serum and liver. DHBAg was observed in the hepatocytes by immunohistochemistry. Histological changes in the liver tissue were also observed. The results demonstrate that ducklings at each time point and in all groups developed detectable viraemia. In each group, DHBV DNA in the liver was at a lower level than in serum and the peak DNA titre was found in serum earlier than in the liver. In the low-dose groups it was always at a lower level than in the high-dose groups. The DHBV replication levels appeared to be directly related to the number of DHBAg-positive hepatocytes. The variation trends of DHBAg-positive hepatocytes were similar in the high-dose groups. Histological changes were associated with liver viral DNA levels. We suggest that this dose and route of inoculation can be used as a model to study acute DHBV infections.

Aim: To study the efficacy of antiviral treatment with PNA for the duck model of HBV (DHBV)-infected ducks. PNA is a 2-amine-9-(2,3-dideoxy-2,3-dihydro-β-D-arabinofuranosyl)-6-methoxy-9H-purine. Methods: The Sichuan Mallard ducklings in the hepatitis B virus model were treated with PNA, a new antiviral agent. DHBV DNA from the blood serum and liver tissues were measured at 0, 5, and 10 d during the treatment and at 3 d withdrawal by real-time PCR. The duck hepatitis B surface antigen (DHBsAg) in the liver cells was observed by Immunohistochemistry (IHC). Pathological changes in the liver tissues were also observed. Control group I was administered with distilled water and control group II was administered with 3-thiacytidine. Treatment group I was administered with PNA at a dose of 40 mg/kg and treatment group II was administered perorally (po) with PNA at a dose of 80 mg/kg. Treatment group III was administered with PNA at a dose of 20 mg/kg and treatment group IV was intravenously administered with PNA at a dose of 40 mg/kg. Each group contained 15 ducklings. Results: PNA can significantly lower the DHBV replication levels in serum and liver. Compared with control group II, there were no significant differences in inhibiting efficacy in treatment groups I and III (P>0.05) and there were significant differences in inhibiting efficacy in treatment groups II and IV (P

Traumatic spinal cord injury (SCI) causes tissue loss and associated neurological dysfunction attributable to both mechanical damage and secondary biochemical and physiological responses. Upregulation of cell cycle proteins occurs in both neurons and glia after SCI and may contribute to these changes. Increased cell cycle protein is associated with neuronal and oligodendroglial apoptosis, reactive astrogliosis, glial scar formation, and microglial activation. Here, using lentiviral vectors (LV), we induced the expression of the cyclin-dependent kinase (CDK) inhibitor p27

This paper presents a two-dimensional (2D) modified cellular automata (CA) algorithm for accurate boundary movement of deposition process simulation. The algorithm incorporates deposition rate calculation methods based on a cell structure. Isotropic and anisotropic deposition processes were successfully simulated by the algorithm, and the simulation profiles show good agreement with available experimental results. The deposition process with defects on the substrate was also implemented using this algorithm. The 2D modified CA algorithm demonstrates accuracy and capability of handling complex boundary and initial conditions. It is useful for the deposition process simulation of microelectromechanical systems and integrated circuits.