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186 results on '"Dabravolski, Siarhei A."'

5. Centromeric localization of KNL2 and CENP-C proteins in plants depends on their centromere-targeting domain and DNA-binding regions

Author

Yalagapati, Surya Prakash, primary, Ahmadli, Ulkar, additional, Sinha, Aditya, additional, Kalidass, Manikandan, additional, Dabravolski, Siarhei, additional, Zuo, Sheng, additional, Yadala, Ramakrishna, additional, Rutten, Twan, additional, Berr, Alexandre, additional, Talbert, Paul, additional, and Lermontova, Inna, additional

Published
2024
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9. The role of centromeric repeats and transcripts in kinetochore assembly and function.

Author

Ramakrishnan Chandra, Jothipriya, Kalidass, Manikandan, Demidov, Dmitri, Dabravolski, Siarhei A., and Lermontova, Inna

Subjects
CENTROMERE, KINETOCHORE, CHROMOSOME segregation, GENETIC transcription regulation, CELL division, PROTEIN-protein interactions
Abstract

SUMMARY: Centromeres are the chromosomal domains, where the kinetochore protein complex is formed, mediating proper segregation of chromosomes during cell division. Although the function of centromeres has remained conserved during evolution, centromeric DNA is highly variable, even in closely related species. In addition, the composition of the kinetochore complexes varies among organisms. Therefore, it is assumed that the centromeric position is determined epigenetically, and the centromeric histone H3 (CENH3) serves as an epigenetic marker. The loading of CENH3 onto centromeres depends on centromere‐licensing factors, chaperones, and transcription of centromeric repeats. Several proteins that regulate CENH3 loading and kinetochore assembly interact with the centromeric transcripts and DNA in a sequence‐independent manner. However, the functional aspects of these interactions are not fully understood. This review discusses the variability of centromeric sequences in different organisms and the regulation of their transcription through the RNA Pol II and RNAi machinery. The data suggest that the interaction of proteins involved in CENH3 loading and kinetochore assembly with centromeric DNA and transcripts plays a role in centromere, and possibly neocentromere, formation in a sequence‐independent manner. Significance Statement: Centromeric DNA regions strongly vary between organisms in length and sequence composition. However, their transcriptional activity is a common feature in different eukaryotes. This review summarizes the evidence for the role of centromeric repeats and transcripts in kinetochore complex assembly. We suggest that the ability of some kinetochore proteins to interact with centromeric DNA and transcripts in a sequence‐independent manner may play an important role in kinetochore assembly at the centromere and neocentromere. [ABSTRACT FROM AUTHOR]

Published
2024
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25. Effect of corn lectins on the intestinal transport of trace elements

Author

Dabravolski, Siarhei A. and Kavalionak, Yury K.

Abstract

The absorption of iron, copper, zinc, and cobalt deriving from the diet and supplements varies, depending on the form of these trace elements and the presence of absorption inhibitors. The objective of this study was to examine the absorption of iron, copper, zinc, and cobalt in the presence of corn lectin as a possible inhibitor of intestinal absorption. The highest level of inhibition was shown in bull in vitro intestinal segments: incubation with corn lectins reduced the absorption of copper by 35.5%, of zinc by 28%, and of cobalt by 17.3%. It caused no observable effect on iron absorption. The addition of N-acetylgalactosamine neutralised the inhibitory effect of corn lectins and restored the absorption of the trace elements. The levels of copper and iron in the blood of rats receiving a diet supplemented with lyophilized corn lectins, decreased by 11.3% and 16.4%, respectively. Our results thus suggest that corn lectins may act as absorption inhibitors of iron, copper, zinc, and cobalt, most likely through blocking the transport of elements across the apical membrane of enterocytes. The present study suggests that individuals should consider their trace element status if their diet includes high amounts of raw corn. However, additional in vivo studies are required to confirm these results.

Published
2024
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35. Recent updates on the physiology and evolution of plant TPK/KCO channels.

Author

Dabravolski, Siarhei A. and Isayenkov, Stanislav V.

Subjects
*PLANT physiology, *PLANT evolution, *PLANT vacuoles, *POTASSIUM channels, *CHLOROPLAST membranes, *CELL membranes, *CHLOROPLASTS
Abstract

Plant vacuoles are the main cellular reservoirs to store K+. The vacuolar K+ channels play a pivotal role in K+ exchange between cytosol and vacuolar sap. Among vacuolar K+ transporters, the Two Pore Potassium Channels (TPKs) are highly selective K+ channels present in most or all plant vacuoles and could be involved in various plant stress responses and developmental processes. Although the majority of TPK members have a vacuolar specialisation, some TPKs display different membrane localisation including the plasma membrane, tonoplast of protein storage vacuoles and probably chloroplast membranes. The functional properties as well as physiological roles of TPKs remains largely unexplored. In this review, we have collected recent data about the physiology, structure, functionality and evolution of TPK/KCO3 channels. We also critically evaluate the latest findings on the biological role, physiological functions, and regulation of TPK/KCO3 channels in relation to their structure and phylogenetic position. The possible role of TPK/KCO3 channels in plant tolerance to various abiotic stresses is summarised, and the future priority directions for TPK/KCO3 studies are addressed. Plant vacuoles are the main cellular compartment to store important minerals such as potassium. The vacuolar Two Pore Potassium Channels (TPKs) play an important role in potassium exchange between cytosol and vacuolar sap. The possible role of these channels in plant tolerance to various abiotic stresses is summarised, the possible evolutional direction is discussed and the future prospective areas for the study of TPK/KCO3 are addressed. [ABSTRACT FROM AUTHOR]

Published
2023
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49. Interplay between Zn 2+ Homeostasis and Mitochondrial Functions in Cardiovascular Diseases and Heart Ageing.

Author

Dabravolski, Siarhei A., Sadykhov, Nikolay K., Kartuesov, Andrey G., Borisov, Evgeny E., Sukhorukov, Vasily N., and Orekhov, Alexander N.

Subjects
*CARDIOVASCULAR diseases, *HOMEOSTASIS, *MYOCARDIAL reperfusion, *MITOCHONDRIA, *HEART development, *HEART abnormalities
Abstract

Zinc plays an important role in cardiomyocytes, where it exists in bound and histochemically reactive labile Zn2+ forms. Although Zn2+ concentration is under tight control through several Zn2+-transporters, its concentration and intracellular distribution may vary during normal cardiac function and pathological conditions, when the protein levels and efficacy of Zn2+ transporters can lead to zinc re-distribution among organelles in cardiomyocytes. Such dysregulation of cellular Zn2+ homeostasis leads to mitochondrial and ER stresses, and interrupts normal ER/mitochondria cross-talk and mitophagy, which subsequently, result in increased ROS production and dysregulated metabolic function. Besides cardiac structural and functional defects, insufficient Zn2+ supply was associated with heart development abnormalities, induction and progression of cardiovascular diseases, resulting in accelerated cardiac ageing. In the present review, we summarize the recently identified connections between cellular and mitochondrial Zn2+ homeostasis, ER stress and mitophagy in heart development, excitation–contraction coupling, heart failure and ischemia/reperfusion injury. Additionally, we discuss the role of Zn2+ in accelerated heart ageing and ageing-associated rise of mitochondrial ROS and cardiomyocyte dysfunction. [ABSTRACT FROM AUTHOR]

Published
2022
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