1. FSH receptor (FSHR) expression in human extragonadal reproductive tissues and the developing placenta, and the impact of its deletion on pregnancy in mice.
- Author
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Stilley JA, Christensen DE, Dahlem KB, Guan R, Santillan DA, England SK, Al-Hendy A, Kirby PA, and Segaloff DL
- Subjects
- Adult, Animals, Cervix Uteri blood supply, Cervix Uteri cytology, Cervix Uteri metabolism, Endometrium blood supply, Endometrium cytology, Endometrium metabolism, Endothelium, Vascular cytology, Extraembryonic Membranes blood supply, Extraembryonic Membranes cytology, Extraembryonic Membranes metabolism, Female, Humans, Immunohistochemistry, Mice, Knockout, Myometrium blood supply, Myometrium cytology, Myometrium metabolism, Placenta blood supply, Placenta cytology, Pregnancy, RNA, Messenger metabolism, Receptors, FSH genetics, Stromal Cells cytology, Stromal Cells metabolism, Umbilical Cord cytology, Umbilical Cord metabolism, Up-Regulation, Endothelium, Vascular metabolism, Gene Expression Regulation, Developmental, Placenta metabolism, Placentation, Receptors, FSH metabolism
- Abstract
Expression and function of the follicle-stimulating hormone receptor (FSHR) in females were long thought to be limited to the ovary. Here, however, we identify extragonadal FSHR in both the human female reproductive tract and the placenta, and test its physiological relevance in mice. We show that in nonpregnant women FSHR is present on: endothelial cells of blood vessels in the endometrium, myometrium, and cervix; endometrial glands of the proliferative and secretory endometrium; cervical glands and the cervical stroma; and (at low levels) stromal cells and muscle fibers of the myometrium. In pregnant women, placental FSHR was detected as early as 8-10 wk of gestation and continued through term. It was expressed on: endothelial cells in fetal portions of the placenta and the umbilical cord; epithelial cells of the amnion; decidualized cells surrounding the maternal arteries in the maternal decidua; and the stromal cells and muscle fibers of the myometrium, with particularly strong expression at term. These findings suggest that FSHR expression is upregulated during decidualization and upregulated in myometrium as a function of pregnancy. The presence of FSHR in the placental vasculature suggests a role in placental angiogenesis. Analysis of genetically modified mice in which Fshr is lacking in fetal portions of the placenta revealed adverse effects on fetoplacental development. Our data further demonstrate FSHB and CGA mRNAs in placenta and uterus, consistent with potential local sources of FSH. Collectively, our data suggest heretofore unappreciated roles of extragonadal FSHR in female reproductive physiology., (© 2014 by the Society for the Study of Reproduction, Inc.)
- Published
- 2014
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