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13 results on '"Dai, Shouqian"'

8. Apolipoprotein C1 (APOC1): A Novel Diagnostic and Prognostic Biomarker for Cervical Cancer

Author

Shi, Xiu, Wang, Juan, Dai, Shouqian, Qin, Lingyan, Zhou, Jinhua, and Chen, Youguo

Subjects
APOC1, cervical cancer, progression, prognosis, OncoTargets and Therapy, Original Research
Abstract

Xiu Shi,1– 3,* Juan Wang,1,* Shouqian Dai,4 Lingyan Qin,5 Jinhua Zhou,1 Youguo Chen1 1Department of Obstetrics and Gynecology, The First Affiliated Hospital of Soochow University, Suzhou, Jiangsu Province, People’s Republic of China; 2Clinical Research Center of Obstetrics and Gynecology, Jiangsu Key Laboratory of Clinical Immunology, Soochow University, Suzhou, Jiangsu Province, People’s Republic of China; 3Jiangsu Institute of Clinical Immunology, The First Affiliated Hospital of Soochow University, Suzhou, Jiangsu Province, People’s Republic of China; 4Department of Emergency Medicine, The First Affiliated Hospital of Soochow University, Suzhou, Jiangsu Province, People’s Republic of China; 5Department of Pathology, The First Affiliated Hospital of Soochow University, Suzhou, Jiangsu Province, People’s Republic of China*These authors contributed equally to this workCorrespondence: Youguo Chen; Jinhua Zhou Email chenyouguo@suda.edu.cn; jsjhzh@126.comBackground: Previous reports showed that APOC1 was associated with several cancers but the function of APOC1 in cervical cancer was unknown. This study aimed to investigate the clinical effect and function of APOC1 in cervical cancer.Materials and Methods: In this study, the relative expression of APOC1 in cervical cancer was detected by RT-qPCR. In order to determine the cell proliferation and migration and invading ability and apoptosis more accurately, we used CCK8 assay, Edu assay, wound healing assay, migration and invasion assay, flow cytometry assay, co-immunoprecipitation, proteomics and Western blot by silencing and overexpressing APOC1, respectively. The role of APOC1 on tumor progression was explored in vitro and vivo.Results: The relative expression of APOC1 in cervical cancer tissues was up-regulated (P< 0.05). In cervical cancer cell lines, silencing of APOC1 restrained cell progression and EMT, while over-expression of APOC1 accelerated cell progression and EMT in vivo and vitro (P< 0.05).Conclusion: APOC1 acts as an oncogene in cervical cancers and knockdown of APOC1 inhibited cervical cancer cells growth in vitro and in vivo. There is a close relationship between the relative expression of APOC1 and clinical outcome in cervical cancer patients.Keywords: APOC1, cervical cancer, progression, prognosis

Published
2020

10. Apolipoprotein C1 (APOC1): A Novel Diagnostic and Prognostic Biomarker for Cervical Cancer

Author

Shi,Xiu, Wang,Juan, Dai,Shouqian, Qin,Lingyan, Zhou,Jinhua, Chen,Youguo, Shi,Xiu, Wang,Juan, Dai,Shouqian, Qin,Lingyan, Zhou,Jinhua, and Chen,Youguo

Abstract

Xiu Shi,1– 3,* Juan Wang,1,* Shouqian Dai,4 Lingyan Qin,5 Jinhua Zhou,1 Youguo Chen1 1Department of Obstetrics and Gynecology, The First Affiliated Hospital of Soochow University, Suzhou, Jiangsu Province, People’s Republic of China; 2Clinical Research Center of Obstetrics and Gynecology, Jiangsu Key Laboratory of Clinical Immunology, Soochow University, Suzhou, Jiangsu Province, People’s Republic of China; 3Jiangsu Institute of Clinical Immunology, The First Affiliated Hospital of Soochow University, Suzhou, Jiangsu Province, People’s Republic of China; 4Department of Emergency Medicine, The First Affiliated Hospital of Soochow University, Suzhou, Jiangsu Province, People’s Republic of China; 5Department of Pathology, The First Affiliated Hospital of Soochow University, Suzhou, Jiangsu Province, People’s Republic of China*These authors contributed equally to this workCorrespondence: Youguo Chen; Jinhua Zhou Email chenyouguo@suda.edu.cn; jsjhzh@126.comBackground: Previous reports showed that APOC1 was associated with several cancers but the function of APOC1 in cervical cancer was unknown. This study aimed to investigate the clinical effect and function of APOC1 in cervical cancer.Materials and Methods: In this study, the relative expression of APOC1 in cervical cancer was detected by RT-qPCR. In order to determine the cell proliferation and migration and invading ability and apoptosis more accurately, we used CCK8 assay, Edu assay, wound healing assay, migration and invasion assay, flow cytometry assay, co-immunoprecipitation, proteomics and Western blot by silencing and overexpressing APOC1, respectively. The role of APOC1 on tumor progression was explored in vitro and vivo.Results: The relative expression of APOC1 in cervical cancer tissues was up-regulated (P< 0.05). In cervical cancer cell lines, silencing of APOC1 restrained cell progression and EMT, while over-expression of APOC1 accelerat

Published
2020

13. YBX-1 alleviates sepsis-stimulated lung epithelial cell injury.

Author

Lu X, Dai S, Li P, Zhou Y, and Xu F

Subjects
Humans, Epithelial Cells, Inflammation metabolism, Lipopolysaccharides pharmacology, Lung, Mitogen-Activated Protein Kinases metabolism, NF-kappa B metabolism, Acute Lung Injury drug therapy, Acute Lung Injury metabolism, Sepsis complications, Sepsis metabolism, Y-Box-Binding Protein 1 metabolism
Abstract

Objective: To explore the role of Y-box binding protein 1 (YBX-1) in the lipopolysaccharide (LPS)-stimulated inflammation and oxidative stress of BEAS-2B cell line and clarify the underlying mechanism., Methods: LPS-stimulated BEAS-2B cells were used as a cell model of sepsis-stimulated acute lung injury (ALI). Immunoblot and quantitative polymerase chain reaction assays were used to detect the expression of YBX-1 in LPS-stimulated BEAS-2B cells. 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide, TdT-mediated dUTP nick end labeling, and immunoblot assays were conducted to determine the effects of YBX-1 on cell survival. JC-1 staining and adenosine triphosphate production were used to detect the effects of YBX-1 on mitochondrial function. Immunostaining and enzyme-linked immunosorbent serologic assay were performed to examine the effects of YBX-1 on the inflammation and oxidative stress of cells. Immunoblot assay was conducted to confirm the mechanism., Results: YBX-1 was lowly expressed in LPS-stimulated BEAS-2B cells and enhanced the survival of LPS-stimulated lung epithelial cells. In addition, YBX-1 improved mitochondrial function of LPS-stimulated BEAS-2B cells. YBX-1 inhibited the inflammation and oxidative stress of LPS-stimulated BEAS-2B cells. Mechanically, YBX-1 inhibited mitogen-activated protein kinase (MAPK) axis, thereby alleviating sepsis-stimulated ALI., Conclusion: YBX-1 alleviated inflammation and oxidative stress of LPS-stimulated BEAS-2B cells via MAPK axis., Competing Interests: The authors states that there were no conflict of interest to disclose.

Published
2024
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