1. Antioxidant, antibacterial and anticancer properties of phyto - synthesised Artemisia quttensis Podlech extract mediated AgNPs.
- Author
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Ghanbar F, Mirzaie A, Ashrafi F, Noorbazargan H, Dalirsaber Jalali M, Salehi S, and Sadat Shandiz SA
- Subjects
- Anti-Bacterial Agents chemical synthesis, Antifungal Agents chemical synthesis, Antineoplastic Agents chemical synthesis, Bacterial Physiological Phenomena drug effects, Dose-Response Relationship, Drug, Drug Compounding methods, Fungi drug effects, HEK293 Cells, HT29 Cells, HeLa Cells, Humans, MCF-7 Cells, Materials Testing, Metal Nanoparticles chemistry, Metal Nanoparticles ultrastructure, Particle Size, Photochemistry methods, Plant Extracts administration & dosage, Plant Extracts chemistry, Silver chemistry, Treatment Outcome, Anti-Bacterial Agents administration & dosage, Antifungal Agents administration & dosage, Antineoplastic Agents administration & dosage, Artemisia chemistry, Cell Survival drug effects, Metal Nanoparticles administration & dosage, Silver administration & dosage
- Abstract
The focus of this study is on a rapid and cost-effective approach for the synthesis of silver nanoparticles (AgNPs) using Artemisia quttensis Podlech aerial parts extract and assessment of their antioxidant, antibacterial and anticancer activities. The prepared AgNPs were determined by ultraviolet-visible spectroscopy, X-ray diffraction, Fourier transform infra-red spectroscopy, transmission electron microscopy, scanning electron microscopy, energy-dispersive spectroscopy, and dynamic light scattering and zeta-potential analysis. The AgNPs and A. quttensis extract were evaluated for their antiradical scavenging activity by 2, 2-diphenyl, 1-picryl hydrazyl assay and anticancer activity against colon cancer (human colorectal adenocarcinoma cell line 29) compared with normal human embryonic kidney (HEK293) cells. Also, the prepared AgNPs were studied for its antibacterial activity. The AgNPs revealed a higher antioxidant activity compared with A. quttensis extract alone. The phyto-synthesised AgNPs and A. quttensis extract showed a dose-response cytotoxicity effect against HT29 and HEK293 cells. As evidenced by Annexin V/propidium iodide staining, the number of apoptotic HT29 cells was significantly enhanced, following treatment with AgNPs as compared with untreated cells. Besides, the antibacterial property of the AgNPs indicated a significant effect against the selected pathogenic bacteria. These present obtained results show the potential applications of phyto-synthesised AgNPs using A. quttensis aerial parts extract.
- Published
- 2017
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