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Abstract Background Gastric cancer is one of the most leading causes of cancer death worldwide. Therefore, treatment studies have been being conducted, one of which is screening of novel agents from medicinal herbs. Elephantopus mollis Kunth (EM) belonging to Asteraceae family is a perennial herb with several therapeutic properties including anticancer activity. However, the effect of this species on gastric cancer has not been reported yet. In this study, cytotoxicity of different EM crude extracts was investigated on AGS gastric cancer cell line. Besides, the effects of extract on nuclear morphology, caspase-3 activation, and gene expression were also explored. Results The results showed that ethyl acetate extract exhibited a remarkably inhibitory ability (IC50 = 27.5 μg/ml) on the growth of AGS cells, while causing less toxicity to normal human fibroblasts. The extract also induced apoptotic deaths in AGS cells as evidenced by cell shrinkage, formation of apoptotic bodies, nuclear fragmentation, caspase-3 activation, and the upregulation of BAK and APAF-1 pro-apoptotic genes related to mitochondrial signaling pathway. Specifically, BAK and APAF-1 mRNA expression levels showed 2.57 and 2.71-fold increases respectively. Conclusions The current study not only proved the anti-gastric cancer activity of EM ethyl acetate extract but also proposed its molecular mechanism. The extract could be a potential candidate for further investigation.

This paper presents the research results on the strength of materials and power of the Toyota 3C engine when changing the structure and number of blades of the compressor wheel in the turbocharger. 3D models of different compressor wheels were created using reverse engineering and then simulated in the ANSYS environment with turbine shaft rotation speeds of 10,000, 15,000, and 20,000 rpm, respectively, to examine the strength of the compression wheel materials. To evaluate engine power, compressor wheels were machined on a 5-axis CNC milling machine. The MP 100S specialized test bed was used to perform experiments and compare engine power when using the original and alternative compressor wheels of the CT9 turbocharger. The compressor wheels were made of aluminum alloy, with a structure and number of blades selected to ensure durability when working. The CT9 turbocharger has a four-pair blade compressor wheel that consistently delivers higher engine power than in other cases. [ABSTRACT FROM AUTHOR]