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Soybean (Glycine max (L.) Merrill) is one of the most important commodities in the world, with grains that show variations in their chemical composition, mainly in oil and protein content. These variations can be related to genotype (G), environment (E), and G × E interaction. Thus, the objectives of this study were to determine the environments which maximize oil and protein yields, and to identify potential soybean cultivars that have increased grain yield, and oil and protein content in high-and low-altitude environments. Twenty-eight soybean cultivars were evaluated at high (846–963 m, Cfb climate) and low (336–480 m, Cfa climate) altitude environments, in the 2017/18 and 2018/19 crops, for grain yield, thousand grain weight, and oil and protein contents and yields. Growing environment affected the chemical composition of soybean grains. Altitude had a positive effect on protein content and a negative effect on oil content. The high-altitude environment increased the protein content by 6.15% (380 g kg-1), whereas the low altitude environment promoted an increase of 5.58% in oil content, with a mean value of 220 g kg-1. We identified soybean cultivars with potential for greater oil and protein yields in high-and low-altitude environments. Knowledge of the associations between environments and the chemical composition of soybean grain is valuable for developing direct breeding efforts, recommending cultivars and growing locations, in order to meet the demand of the oil and protein market

UFSMFW 2202 is an early black oat cultivar with high dry matter and grain yield potential. When used in rotation systems, the early cycle has the immediate advantage of allowing anticipated sowing of subsequent crop. Initially, the cultivar is recommended for the North and Northwest of Rio Grande do Sul.

Asian soybean rust (ASR) causes large reductions in soybean yield, affecting the entire grain market. With low fungicide efficiency, the use of resistant cultivars can be an economical, safe, efficient, and sustainable control alternative. However, the great variability and aggressiveness of ASR and the use of Rpp genes are limited. Thus, gene pyramiding is a promising strategy for the development of cultivars with high resistance to a greater number of isolates. Thus, the aim of this study was to evaluate sister lines with different pyramided Rpp gene for resistance to Phakopsora pachyrhizi and identify which combination of Rpp genes had higher levels of resistance under field conditions. All Rpp-pyramided lines showed higher levels of resistance, with significant reductions in sporulation levels (SL), number of uredinia per lesion (NoU), and frequency of lesions with uredinia (%LU), compared to the resistance sources PI200487 (Rpp5), PI200492 (Rpp1), PI230970 (Rpp2), PI459025A (Rpp4), PI506764 (Rpp3, 5), PI587880A (Rpp1-b), PI594538A (Rpp1-b), and PI594723 (Rpp1-b). Rpp-pyramided lines carrying Rpp1-b + Rpp1-b, Rpp2 + Rpp1-b, Rpp4 + Rpp1-b, and single gene Rpp1-b were classified as “highly resistant”. Furthermore, one sister line, 52117-57 (Rpp2 + Rpp1-b), showed immunity under field conditions. The Rpp-pyramided genes are an alternative for achieving high resistance levels against ASR.

The black oat cultivar UFSMFW 2101, recommended for Northwest of the state of Rio Grande do Sul, is very early and can produce high dry matter and grain yields. In rotation systems, an immediate advantage is the very early cycle, with the possibility of anticipated sowing of the subsequent crop.

This study aimed to identify important traits for indirect selection and to evaluate the variability among black oat populations through cause and effect relationships and canonical variables. Fourteen (14) black oat populations were collected in the 2013 cropping season which were evaluated in the laboratory, and then in the field in the 2014 cropping season. The seed width has a high and positive association with physiological quality of black oat seeds. The number of grains and thousand-grain weight has greater direct effects on the grain yield of black oat; thus, these traits may be considered for indirect selection in earlier phases of future black oat breeding programs. Targeted crosses between black oat genotypes coming from Salvador das Missoes with genotypes coming from the other studied locations should be promising to obtain recombinant offspring in the future.

The objective of this work was to assess the breeding influences in different agronomic and physiological traits in Brazilian soybean cultivars, released between 1965 and 2011, to identify traits associated with modern cultivars. A total of 29 cultivars were evaluated in two locations in the 2016/17 crop season. Genotype selection based on agronomic and physiological traits was determined using GYT (Grain Yield*Trait) methodology, which uses the Superiority Index to rank genotypes by mean of all traits. Grain Yield is combined with other target traits and shows the strengths and weaknesses of each genotype. Soybean breeding improved desirable traits during the 46 years of evaluation. Superiority index can be a powerful tool for breeders to obtain high genetic gains in the future. The cultivars DMario 58i, TMG 7161RR and TMG 7262 RR stand out as the best cultivars but present different sets of desirable traits. The traits grain yield, harvest index, number of pods per plant, reproductive-vegetative ratio, photosynthetic rate and transpiration rate are core traits which can be evaluated in soybean breeding programs.

In this study, we aim to develop and validate KASP molecular markers in soybean populations for Asian soybean rust (ASR) resistance gene Rpp1 (PI 200492, PI 594538A, PI 587880A), identify the gene hypothetically present in PI 594723, and validate KASP markers for Rpp2 (PI 230970), Rpp3 (PI 506764), Rpp4 (PI 459025A), and Rpp5 (PI 506764, PI 200487). Ten F2 soybean (Glycine max (L.) Merrill) populations derived from crosses between rust-susceptible (55I57RSF IPRO, 63I64RSF IPRO) x rust-resistant sources (PI 200492, PI 594738A, PI 587880A, PI 594723, PI 230970, PI 506764, PI 459025A and PI 200487) were evaluated. All F2 plants were individually evaluated in field conditions for ASR phenotypic reactions, classified according to sporulation level. SNP markers were developed according to markers associated with Rpp genes available at the SoyBase, using KASP methodology. Based on a slight difference in map position and different phenotypic disease reactions of PI 200492, the authors suggest that PI 594723 carries a resistance gene Rpp1-b. The Rpp1-b gene from PI 594723 was mapped in Chr 18 in a 12.4 cM region. The PIs carrying Rpp1-b (PI 594723, PI 587880A, and 594538A) showed strong resistance to ASR compared to the lines carrying Rpp1 (PI 200492). A total of 26 KASP markers were significantly associated (P Rpp1), M13 and M14 (Rpp2), M16, M17 and M20 (Rpp3), M25 and M26 (Rpp4), and M27 and M28 (Rpp5) have the potential to be used in marker-assisted selection strategies.

Risk assessment is an important management tool that is required by different management standards to support manufacturing companies in the identification of improvement actions to prevent the occurrence of undesirable events. This paper defines the specifications of a dynamic and collaborative risk assessment system for manufacturing processes. This tool allows overcoming the usual limitations of the risk assessment process in companies, namely: the departmental risk analysis, which increases the time of execution and hinders the identification of the road map that leads to the occurrence of a certain undesirable event; and the lack of updating and subjectivity of the evaluation that lead to decision making based on unreliable data. The overcoming of these limitations is made possible by the connection between records of undesirable events and the risks assessment process and by collaboration between departments or areas, ensured by the proposed system., FCT - Fundação para a Ciência e a Tecnologia(UIDB/00319/2020)

In this study, we summarized the evolution of scientific research about fungus Phakopsora pachyrhizi Sydow & Sydow affecting soybean [Glycine max (L.) Merrill]. We retrieved research articles from the Thomson Reuters Web of Science database that reported studies of the Asian soybean rust (ASR) from 1945 to 2018. We applied the scientometric analysis of the temporal trends of authors, journals, research partnerships, and resistance genes to ASR. Several studies aiming to understand fungal etiology, testing, or improve strategies to control the pathogen were developed. The temporal trends in publications showed an increase in the number of publications between 2005 and 2013. This is directly related to the dissemination of ASR in South and later North America, which facilitates field research and an increase in grant opportunities. The USA had the highest number of published articles (42%), followed by Brazil, Germany, Japan, and Argentina. The leading organizations associated with these publications are from Brazil and the USA. 19% of articles pertaining to ASR were published in the journal Plant Disease. Our results also showed that among the seven dominant genes identified for resistance to ASR, Rpp1, and Rpp2 are the most studied. Several countries, institutions, and plant scientists are deeply involved in ASR research and have been making efforts to control this disease and ensure food security worldwide.

The aims of this study were to characterize black oat populations by estimating between- and within-populations variance components and genetic parameters, as well as to distinguish the populations using multivariable statistics. The experiment was carried out in randomized blocks design with three repetitions, with 14 black oat populations collected in several municipalities of Rio Grande do Sul state. Agronomic important traits were assessed at physiological maturation stage. Variance components and genetic parameters were estimated considering within-block information. In addition, Mahalanobis distance, relative contribution of traits, and canonical variables were used to distinguish the populations. Selection based on panicle length, number of grains per panicle, panicle weight and panicle grain weight may result in higher selection gains. Panicle grain weight presents a greater contribution to genetic divergence between studied populations. The formation of distinct groups indicated the presence of genetic variability among black oat populations in the northwestern of the Rio Grande do Sul state. Directed crosses between individual plants of populations from (i) Alto Alegre and Salvador das Missoes, Chapada, or Santa Rosa or (ii) between plants of populations from Salvador das Missoes and Campos Borges or Santa Rosa can generate segregating populations with great genetic variability. The predominance of between-phenotypic variance and a within-genetic variance indicate prospects for success in selection gain and possible selection of a new cultivar with fewer efforts compared to a cross-based method. This is supported by the high values of within-population heritability.

The aim of this study was to select growth models to describe strawberry (Fragaria x ananassa)fruit production. To do this, data on 16 treatments (combination of 2 cultivars [Albion and Camarosa], ...

The lack of research about escarole cultivars adapted to each region, as well as technical information on plant growth have been some of the factors related to low yields. Therefore, the aim of this work was to evaluate the growth of escarole crop in four seasons of year in Santa Maria, RS. The experiment was conducted in a randomized complete block design, 1 x 4, being a escarole (cultivar Escorola Lisa®) evaluated in four seasons of year (summer, autumn, winter and spring), in six replications. The evaluations were performed in a destructive manner every seven days, from date of transplant to harvest point. The following components were analyzed: number of leaves, number of residues, dry mass of leaves, dry mass of leaves, stem and residue. Thus, it was determined the parameters for growth analysis. With the data obtained, we performed a variance analysis, and presented significant results, and the means of each season of the year submitted to the Tukey test at 5% of error probability. The air temperature and global radiation influence crop cycle. Lower temperatures and lower incidence of radiation result in cycle extended, as observed in autumn. The escarole growth is influenced by seasons of year in Rio Grande do Sul (RS), and summer and spring finish the cycle in advance to others, presenting a larger leaf area. Summer and spring are most indicated season to escarole crop in RS, Brazil.

The aim of work was to verify the effect of different shading levels in escarole crop, during summer in the northwest of Rio Grande do Sul. The experiment was conducted at the Federal University of Santa Maria, Frederico Westphalen RS Campus, in 2015 with Escarola Lisa escarole cultivar. The experimental design was a randomized complete block design with three shading levels (0%, 30%, 50%), with six replications. Growth evaluation were performed in destructive manner every seven days, from transplant to harvest point. From the information obtained, traits were determined: leaf area, leaf area index, leaf area ratio, specific leaf area, leaf weight ratio, biological productivity, absolute growth rate, relative growth rate, net assimilation rate. The data obtained were analyzed by the statistical program Genes, the Tukey test at 5% of error probability. The shading level 30% provided greater leaf area, higher biological productivity and absolute growth rate. However, the leaf area ratio was higher when shading level 50% was tested. However, it can be said that the shading levels favor growth of escarole crop.

El ácido salicílico (AS) es conocido por estar involucrado en una serie de procesos fisiológicos que mejoran las características de germinación y vigor. El objetivo de este trabajo fue evaluar el efecto de concentraciones de AS sobre la calidad fisiológica de semillas de escarola (Cichorium endivia L.) cv. Escarola Lisa. Las semillas de escarola fueron embebidas durante 24 horas en concentraciones 0; 0,05; 0,1; 0,15 y 0,2 mM de Bion® (análogo al ácido salicílico) y después sometidas a pruebas de germinación y vigor (laboratorio e invernadero). Los lotes de semillas evaluadas diferían en cuanto a su calidad fisiológica. El uso de ácido salicílico no presenta beneficios a la calidad fisiológica de semillas de escarola. Sin embargo, se sugiere que nuevos trabajos sean realizados probando este producto en condiciones de estrés o aplicado en estadios posteriores a la germinación de las semillas.

The wheat cultivar UTF 25 is recommended for wheat-growing regions 1 and 2 of Paraná and Santa Catarina states. It has a good disease resistance, early cycle, and high grain yield potential, with an average yield of 3.511 kg ha-1. It is classified as bread wheat with white flour.

Statement of problem An intraradicular retainer formed by multiple independent glass fiber filaments was developed aiming to allow better adaptation in flattened root canals; however, the performance of the new posts is unclear. Purpose The purpose of this in vitro study was to compare the bond strength (BS) and adhesive interface quality achieved in flattened root canals restored with conventional glass fiber posts (CFPs) and multifilament glass fiber posts (MFPs). Material and methods The distal roots of mandibular molars with long oval root canals were endodontically treated, and the obturation material was removed and assigned to 2 groups (n=11) according to the type of retainer used: CFP (WhitePostDC#0.5; FGM) or MFP (CometTail#4; Synca). The posts were cemented with self-adhesive resin cement. The specimens were sectioned (2 slices per third). The most cervical slice in each third was used to evaluate the BS, while the adhesive interface in the apical slices was analyzed by scanning electron microscopy. BS data were analyzed by using a multilevel generalized linear model, and adhesive interface SEM data were analyzed by using a multilevel ordinal logistic regression model (α=.05). Results Multilevel regression showed a statistically significant difference for the “type of retainer” factor (P=.001; CFP 2.61 ±1.30>MFP 1.59 ±1.54). No statistically significant differences were found for the “root thirds” factor (P=.346) or for the interaction of both factors (P=.114). The failure pattern was predominantly mixed or adhesive for CFP and adhesive to dentin for MFP. A better adaptation of the restorative material was observed in the cervical third for CFP and in the apical third for MFP (P Conclusions MFP resulted in lower BS values than CFP, with a higher prevalence of adhesive failures to dentin and better adaptation of the adhesive interface in the apical third.

An optimal number of test locations and replications in yield trials allows improvements in the efficiency of a breeding program and selection reliability. The objectives of this study were to verify the optimal number of test locations and replications per test location in edaphoclimatic regions (ECR) and macroregions (MRs) of soybean adaptation in Brazil. Grain yield data from 184 soybean trials in 57 locations was evaluated in crop seasons 2012–2013 to 2015–2016. The number of replications currently used (3) is sufficient to achieve selection reliability and reduce costs in all MRs. However, the optimal number of replications needs to be determined for each location. Locations that require a high number of replicates to achieve H = 0.75 must be removed and/or replaced to improve selective accuracy. The optimal mean number of test locations in macroregions MR1 and MR2 is seven locations per ECR, and in MR3 and MR4, it is five locations per ECR. Thus, analyses of the optimal number of locations and replications may be applied to other regions to improve the yield trial efficiency of soybean cultivars.

Statement of problem Data comparing the fatigue performance of adhesively luted glass or polycrystalline ceramic systems for computer-aided design and computer-aided manufacturing (CAD-CAM) are scarce. Purpose The purpose of this in vitro study was to evaluate and compare the fatigue performance of monolithic crowns manufactured from glass or polycrystalline CAD-CAM ceramic systems adhesively luted to a dentin analog. Materials and Methods Fifty-four pairs of standardized preparations of dentin analog (NEMA Grade G10) and simplified ceramic crowns of 1.5-mm thickness were obtained with 3 ceramic materials: lithium disilicate (LD) glass-ceramic (IPS e.max CAD); zirconia-reinforced lithium silicate (ZLS) glass-ceramic (Vita Suprinity); and translucent yttrium fully stabilized polycrystalline zirconia (Trans YZ) (Prettau Anterior). The simplified crowns (n=15) were adhesively cemented onto the preparations and subjected to step-stress fatigue test (initial load of 400 N, 20 Hz, 10 000 cycles, followed by 100-N increment steps until failure). Collected data (fatigue failure load [FFL] and cycles for failure [CFF]) were submitted to survival analysis with the Kaplan-Meier and Mantel-Cox post hoc tests (α=.05) and to Weibull analysis (Weibull modulus and its respective 95% confidence interval). Failed crowns were submitted to fractography analysis. The surface characteristics of the internal surface (roughness, fractal dimension) of additional crowns were accessed, and the occlusal cement thickness obtained in each luted system was measured. Results Trans YZ crowns presented the highest values of FFL, CFF, and survival rates, followed by ZLS and LD (mean FFL: 1740 N>1187 N>987 N; mean CFF: 149 000>92 613>73 667). Weibull modulus and cement thickness were similar for all tested materials. LD presented the roughest internal surface, followed by ZLS (mean Ra: 226 nm>169 nm>93 nm). The LD and ZLS internal surfaces also showed higher fractal dimension, pointing to a more complex surface topography (mean fractal dimension: 2.242=2.238>2.147). Conclusions CAD-CAM monolithic crowns of Trans YZ show the best fatigue performance. In addition, ZLS crowns also showed better performance than LD crowns.