Author: "Dardelle, Flavien" - Searchworks@Jio Institute Digital Library Search Results

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49 results on '"Dardelle, Flavien"'

1. Diversity, Complexity, and Specificity of Bacterial Lipopolysaccharide (LPS) Structures Impacting Their Detection and Quantification

Author: Dardelle, Flavien, primary, Phelip, Capucine, additional, Darabi, Maryam, additional, Kondakova, Tatiana, additional, Warnet, Xavier, additional, Combret, Edyta, additional, Juranville, Eugenie, additional, Novikov, Alexey, additional, Kerzerho, Jerome, additional, and Caroff, Martine, additional
Published: 2024
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2. A chemical screen identifies two novel small compounds that alter Arabidopsis thaliana pollen tube growth

Author: Laggoun, Ferdousse, Dardelle, Flavien, Dehors, Jérémy, Falconet, Denis, Driouich, Azeddine, Rochais, Christophe, Dallemagne, Patrick, Lehner, Arnaud, and Mollet, Jean-Claude
Published: 2019
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3. Pollen tube cell walls of wild and domesticated tomatoes contain arabinosylated and fucosylated xyloglucan

Author: Dardelle, Flavien, Le Mauff, François, Lehner, Arnaud, Loutelier-Bourhis, Corinne, Bardor, Muriel, Rihouey, Christophe, Causse, Mathilde, Lerouge, Patrice, Driouich, Azeddine, and Mollet, Jean-Claude
Published: 2015
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4. Unity Is Strength: The Power of Border Cells and Border-Like Cells in Relation with Plant Defense

Author: Driouich, Azeddine, Cannesan, Marc-Antoine, Dardelle, Flavien, Durand, Caroline, Plancot, Barbara, Bernard, Sophie, Follet-Gueye, Marie-Laure, Vicré-Gibouin, Maïté, Vivanco, Jorge M., editor, and Baluška, František, editor
Published: 2012
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5. Biochemical and Immunocytological Characterizations of Arabidopsis Pollen Tube Cell Wall

Author: Dardelle, Flavien, Lehner, Arnaud, Ramdani, Yasmina, Bardor, Muriel, Lerouge, Patrice, Driouich, Azeddine, and Mollet, Jean-Claude
Published: 2010

6. Unity Is Strength: The Power of Border Cells and Border-Like Cells in Relation with Plant Defense

Author: Driouich, Azeddine, primary, Cannesan, Marc-Antoine, additional, Dardelle, Flavien, additional, Durand, Caroline, additional, Plancot, Barbara, additional, Bernard, Sophie, additional, Follet-Gueye, Marie-Laure, additional, and Vicré-Gibouin, Maïté, additional
Published: 2011
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7. Transcriptomic and metabolomic adaptation of Nannochloropsis gaditana grown under different light regimes

Author: Patelou, Maria, primary, Infante, Carlos, additional, Dardelle, Flavien, additional, Randewig, Dörte, additional, Kouri, Evangelia D., additional, Udvardi, Michael K., additional, Tsiplakou, Eleni, additional, Mantecón, Lalia, additional, and Flemetakis, Emmanouil, additional
Published: 2020
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8. Additional file 1: of A chemical screen identifies two novel small compounds that alter Arabidopsis thaliana pollen tube growth

Author: Ferdousse Laggoun, Dardelle, Flavien, Dehors, Jérémy, Falconet, Denis, Azeddine Driouich, Rochais, Christophe, Dallemagne, Patrick, Lehner, Arnaud, and Mollet, Jean-Claude
Abstract: Figure S1. Dose-response effect of the compounds on (a) Solanum lycopersicum and (b) Nicotiana tabacum pollen tubes after 6 h of culture. (PDF 3876 kb)
Published: 2019
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9. Additional file 2: of A chemical screen identifies two novel small compounds that alter Arabidopsis thaliana pollen tube growth

Author: Ferdousse Laggoun, Dardelle, Flavien, Dehors, Jérémy, Falconet, Denis, Azeddine Driouich, Rochais, Christophe, Dallemagne, Patrick, Lehner, Arnaud, and Mollet, Jean-Claude
Abstract: Figure S2. Time-lapse observations of actin dynamics in Arabidopsis thaliana pollen tubes treated for 2, 4 and 6 h with the compounds. (PDF 220 kb)
Published: 2019
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10. Additional file 7: of A chemical screen identifies two novel small compounds that alter Arabidopsis thaliana pollen tube growth

Author: Ferdousse Laggoun, Dardelle, Flavien, Dehors, Jérémy, Falconet, Denis, Azeddine Driouich, Rochais, Christophe, Dallemagne, Patrick, Lehner, Arnaud, and Mollet, Jean-Claude
Abstract: Figure S4. Time-lapse imaging of RIC4 dynamics of a pollen tube treated with Disruptol-B for 2 h. (PDF 169 kb)
Published: 2019
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11. Arabidopsis pollen tube growth and adhesion using an enzymatic approach

Author: Laggoun, Ferdousse, Dardelle, Flavien, Driouich, Azeddine, Lerouge, Patrice, Lehner, Arnaud, Mollet, Jean-Claude, Laboratoire de Glycobiologie et Matrice Extracellulaire Végétale (Glyco-MEV), Université de Rouen Normandie (UNIROUEN), Normandie Université (NU)-Normandie Université (NU), and Lehner, Arnaud
Subjects: [SDV.BDD.GAM] Life Sciences [q-bio]/Development Biology/Gametogenesis, [SDV.BIO]Life Sciences [q-bio]/Biotechnology, [SDV.BBM.BM]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Molecular biology, [SDV.BBM.MN]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Molecular Networks [q-bio.MN], [SDV.BC.BC]Life Sciences [q-bio]/Cellular Biology/Subcellular Processes [q-bio.SC], [SDV.BC.IC] Life Sciences [q-bio]/Cellular Biology/Cell Behavior [q-bio.CB], [SDV.BBM.BM] Life Sciences [q-bio]/Biochemistry, Molecular Biology/Molecular biology, [SDV.BIO] Life Sciences [q-bio]/Biotechnology, [SDV.BV.PEP]Life Sciences [q-bio]/Vegetal Biology/Phytopathology and phytopharmacy, [SDV.BV.AP]Life Sciences [q-bio]/Vegetal Biology/Plant breeding, [SDV.BBM.MN] Life Sciences [q-bio]/Biochemistry, Molecular Biology/Molecular Networks [q-bio.MN], [SDV.BBM.GTP]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Genomics [q-bio.GN], [SDV.BC.IC]Life Sciences [q-bio]/Cellular Biology/Cell Behavior [q-bio.CB], [SDV.BC.BC] Life Sciences [q-bio]/Cellular Biology/Subcellular Processes [q-bio.SC], [SDV.BBM.GTP] Life Sciences [q-bio]/Biochemistry, Molecular Biology/Genomics [q-bio.GN], [SDV.BV]Life Sciences [q-bio]/Vegetal Biology, [SDV.BV.AP] Life Sciences [q-bio]/Vegetal Biology/Plant breeding, [SDV.BV] Life Sciences [q-bio]/Vegetal Biology, [SDV.BBM.BC]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Biochemistry [q-bio.BM], [SDV.BBM.BC] Life Sciences [q-bio]/Biochemistry, Molecular Biology/Biochemistry [q-bio.BM], [SDV.BV.PEP] Life Sciences [q-bio]/Vegetal Biology/Phytopathology and phytopharmacy, ComputingMilieux_MISCELLANEOUS, [SDV.BDD.GAM]Life Sciences [q-bio]/Development Biology/Gametogenesis
Abstract: International audience
Published: 2017

12. Arabidopsis pollen tube growth and adhesion: Dissecting the pectin structure using an enzymatic approach

Author: Laggoun, Ferdousse, Dardelle, Flavien, Driouich, Azeddine, Lerouge, Patrice, Lehner, Arnaud, Mollet, Jean-Claude, Lehner, Arnaud, Laboratoire de Glycobiologie et Matrice Extracellulaire Végétale (Glyco-MEV), Université de Rouen Normandie (UNIROUEN), and Normandie Université (NU)-Normandie Université (NU)
Subjects: [CHIM.POLY] Chemical Sciences/Polymers, [SDV.BDD.GAM] Life Sciences [q-bio]/Development Biology/Gametogenesis, [SDV.BIO]Life Sciences [q-bio]/Biotechnology, [SDV.BBM.BM]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Molecular biology, [SDV.BBM.MN]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Molecular Networks [q-bio.MN], [SDV.BC.BC]Life Sciences [q-bio]/Cellular Biology/Subcellular Processes [q-bio.SC], [SDV.BC.IC] Life Sciences [q-bio]/Cellular Biology/Cell Behavior [q-bio.CB], [SDV.BBM.BM] Life Sciences [q-bio]/Biochemistry, Molecular Biology/Molecular biology, [SDV.BV.PEP]Life Sciences [q-bio]/Vegetal Biology/Phytopathology and phytopharmacy, [SDV.BIO] Life Sciences [q-bio]/Biotechnology, [SDV.BV.AP]Life Sciences [q-bio]/Vegetal Biology/Plant breeding, [CHIM.POLY]Chemical Sciences/Polymers, [SDV.BBM.MN] Life Sciences [q-bio]/Biochemistry, Molecular Biology/Molecular Networks [q-bio.MN], [SDV.BBM.GTP]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Genomics [q-bio.GN], [SDV.BC.IC]Life Sciences [q-bio]/Cellular Biology/Cell Behavior [q-bio.CB], [SDV.BC.BC] Life Sciences [q-bio]/Cellular Biology/Subcellular Processes [q-bio.SC], [SDV.BBM.GTP] Life Sciences [q-bio]/Biochemistry, Molecular Biology/Genomics [q-bio.GN], [SDV.BV.AP] Life Sciences [q-bio]/Vegetal Biology/Plant breeding, [SDV.BBM.BC]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Biochemistry [q-bio.BM], [SDV.BBM.BC] Life Sciences [q-bio]/Biochemistry, Molecular Biology/Biochemistry [q-bio.BM], [SDV.BDD.GAM]Life Sciences [q-bio]/Development Biology/Gametogenesis, ComputingMilieux_MISCELLANEOUS, [SDV.BV.PEP] Life Sciences [q-bio]/Vegetal Biology/Phytopathology and phytopharmacy
Abstract: International audience
Published: 2016

13. UUAT1: an UDP-URONIC ACID TRANSPORTER involved in cell wall composition

Author: Saez-Aguayo, Susana, Rautengarten, Carsten, Ejsmentewicz, Troy, Sanhueza, Dayan, Sandoval, Omar, Temple, Henry, Doinas-Cofré, Daniela, Dardelle, Flavien, Mollet, Jean-Claude, Heazelwood, Joshua, Scheller, Henrik V., Reyes, Francisca, Orellana, Ariel, Lehner, Arnaud, Universidad Andrés Bello [Santiago] (UNAB), University of Melbourne, Laboratoire de Glycobiologie et Matrice Extracellulaire Végétale (Glyco-MEV), Université de Rouen Normandie (UNIROUEN), Normandie Université (NU)-Normandie Université (NU), Lawrence Berkeley National Laboratory [Berkeley] (LBNL), and Universidad Andrés Bello - UNAB (CHILE)
Subjects: [CHIM.POLY] Chemical Sciences/Polymers, [SDV.BDD.GAM] Life Sciences [q-bio]/Development Biology/Gametogenesis, [SDV.BIO]Life Sciences [q-bio]/Biotechnology, [SDV.BBM.BM]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Molecular biology, [SDV.BBM.MN]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Molecular Networks [q-bio.MN], [SDV.BC.BC]Life Sciences [q-bio]/Cellular Biology/Subcellular Processes [q-bio.SC], [SDV.BC.IC] Life Sciences [q-bio]/Cellular Biology/Cell Behavior [q-bio.CB], [SDV.BBM.BM] Life Sciences [q-bio]/Biochemistry, Molecular Biology/Molecular biology, [SDV.BV.PEP]Life Sciences [q-bio]/Vegetal Biology/Phytopathology and phytopharmacy, [SDV.BIO] Life Sciences [q-bio]/Biotechnology, [SDV.BV.AP]Life Sciences [q-bio]/Vegetal Biology/Plant breeding, [CHIM.POLY]Chemical Sciences/Polymers, [SDV.BBM.MN] Life Sciences [q-bio]/Biochemistry, Molecular Biology/Molecular Networks [q-bio.MN], [SDV.BBM.GTP]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Genomics [q-bio.GN], [SDV.BC.IC]Life Sciences [q-bio]/Cellular Biology/Cell Behavior [q-bio.CB], [SDV.BC.BC] Life Sciences [q-bio]/Cellular Biology/Subcellular Processes [q-bio.SC], [SDV.BBM.GTP] Life Sciences [q-bio]/Biochemistry, Molecular Biology/Genomics [q-bio.GN], [SDV.BV.AP] Life Sciences [q-bio]/Vegetal Biology/Plant breeding, [SDV.BBM.BC]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Biochemistry [q-bio.BM], [SDV.BBM.BC] Life Sciences [q-bio]/Biochemistry, Molecular Biology/Biochemistry [q-bio.BM], [SDV.BDD.GAM]Life Sciences [q-bio]/Development Biology/Gametogenesis, ComputingMilieux_MISCELLANEOUS, [SDV.BV.PEP] Life Sciences [q-bio]/Vegetal Biology/Phytopathology and phytopharmacy
Abstract: International audience
Published: 2015

14. Small molecules interfere with the tip-polarized growth of Arabidopsis and tomato pollen

Author: Laggoun, Ferdousse, Dardelle, Flavien, Falconet, Denis, Lesnard, Aurélien, Rault, Sylvain, Driouich, Azeddine, Lerouge, Patrice, Lehner, Arnaud, Mollet, Jean-Claude, Laboratoire de Glycobiologie et Matrice Extracellulaire Végétale (Glyco-MEV), Université de Rouen Normandie (UNIROUEN), Normandie Université (NU)-Normandie Université (NU), Physiologie cellulaire et végétale (LPCV), Institut National de la Recherche Agronomique (INRA)-Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes (UGA)-Institut de Recherche Interdisciplinaire de Grenoble (IRIG), Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA), Centre d'Etudes et de Recherche sur le Médicament de Normandie (CERMN), Université de Caen Normandie (UNICAEN), Laboratoire de physiologie cellulaire végétale (LPCV), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Institut National de la Recherche Agronomique (INRA)-Université Joseph Fourier - Grenoble 1 (UJF)-Centre National de la Recherche Scientifique (CNRS), and Université Joseph Fourier - Grenoble 1 (UJF)-Institut National de la Recherche Agronomique (INRA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche Interdisciplinaire de Grenoble (IRIG)
Subjects: [SDV.BV.AP]Life Sciences [q-bio]/Vegetal Biology/Plant breeding, [SDV.BIO]Life Sciences [q-bio]/Biotechnology, [SDV.BBM.GTP]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Genomics [q-bio.GN], [SDV.BC.IC]Life Sciences [q-bio]/Cellular Biology/Cell Behavior [q-bio.CB], [SDV.BV]Life Sciences [q-bio]/Vegetal Biology, [SDV.BBM.MN]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Molecular Networks [q-bio.MN], [SDV.BBM.BM]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Molecular biology, [SDV.BC.BC]Life Sciences [q-bio]/Cellular Biology/Subcellular Processes [q-bio.SC], [SDV.BBM.BC]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Biochemistry [q-bio.BM], ComputingMilieux_MISCELLANEOUS, [SDV.BDD.GAM]Life Sciences [q-bio]/Development Biology/Gametogenesis, [SDV.BV.PEP]Life Sciences [q-bio]/Vegetal Biology/Phytopathology and phytopharmacy, [SDV.BBM.BC]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Biomolecules [q-bio.BM]
Abstract: International audience
Published: 2014

15. Small molecules interfere with the tip-polarized growth of Arabidopsis and tomato pollen tubes

Author: Laggoun, Ferdousse, Dardelle, Flavien, Falconet, Denis, Driouich, Azeddine, Lerouge, Patrice, Lehner, Arnaud, Mollet, Jean-Claude, Laboratoire de Glycobiologie et Matrice Extracellulaire Végétale (Glyco-MEV), Université de Rouen Normandie (UNIROUEN), Normandie Université (NU)-Normandie Université (NU), Laboratoire de physiologie cellulaire végétale (LPCV), Université Joseph Fourier - Grenoble 1 (UJF)-Institut National de la Recherche Agronomique (INRA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche Interdisciplinaire de Grenoble (IRIG), Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA), Physiologie cellulaire et végétale (LPCV), Institut National de la Recherche Agronomique (INRA)-Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes (UGA)-Institut de Recherche Interdisciplinaire de Grenoble (IRIG), and Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Institut National de la Recherche Agronomique (INRA)-Université Joseph Fourier - Grenoble 1 (UJF)-Centre National de la Recherche Scientifique (CNRS)
Subjects: [SDV.BV.AP]Life Sciences [q-bio]/Vegetal Biology/Plant breeding, [SDV.BIO]Life Sciences [q-bio]/Biotechnology, [SDV.BBM.GTP]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Genomics [q-bio.GN], [SDV.BC.IC]Life Sciences [q-bio]/Cellular Biology/Cell Behavior [q-bio.CB], [SDV.BV]Life Sciences [q-bio]/Vegetal Biology, [SDV.BBM.MN]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Molecular Networks [q-bio.MN], [SDV.BBM.BM]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Molecular biology, [SDV.BC.BC]Life Sciences [q-bio]/Cellular Biology/Subcellular Processes [q-bio.SC], [SDV.BBM.BC]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Biochemistry [q-bio.BM], ComputingMilieux_MISCELLANEOUS, [SDV.BDD.GAM]Life Sciences [q-bio]/Development Biology/Gametogenesis, [SDV.BBM.BC]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Biomolecules [q-bio.BM], [SDV.BV.PEP]Life Sciences [q-bio]/Vegetal Biology/Phytopathology and phytopharmacy
Abstract: International audience
Published: 2014

16. Nucleotide sugar transporters are involved in the biosynthesis of rhamnose- and arabinose-containing cell wall polysaccharides

Author: Temple, Henry, Moreno, Ignacio, Donas-Cofré, Daniela, Blanco, Franscisca, Greve, M., Moreno, Adrian, Dardelle, Flavien, Mortimer, J., Rautengarten, Carsten, Mollet, Jean-Claude, Dupree, Paul, Scheller, Henrik V., Orellana, Ariel, Lehner, Arnaud, Universidad Andrés Bello [Santiago] (UNAB), Laboratoire de Glycobiologie et Matrice Extracellulaire Végétale (Glyco-MEV), Université de Rouen Normandie (UNIROUEN), Normandie Université (NU)-Normandie Université (NU), Joint BioEnergy Institute [Emeryville], University of Melbourne, Department of Biochemistry [Cambridge], University of Cambridge [UK] (CAM), Lawrence Berkeley National Laboratory [Berkeley] (LBNL), and Millennium Nucleus in Plant Cell Biology and Plant Biotechnology Center
Subjects: [SDV.BIO]Life Sciences [q-bio]/Biotechnology, [SDV.BBM.BM]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Molecular biology, [SDV.BC]Life Sciences [q-bio]/Cellular Biology, [SDV.BC.BC]Life Sciences [q-bio]/Cellular Biology/Subcellular Processes [q-bio.SC], [SDV.BC.IC] Life Sciences [q-bio]/Cellular Biology/Cell Behavior [q-bio.CB], [SDV.BBM.BM] Life Sciences [q-bio]/Biochemistry, Molecular Biology/Molecular biology, [SDV.BV.PEP]Life Sciences [q-bio]/Vegetal Biology/Phytopathology and phytopharmacy, [SDV.BIO] Life Sciences [q-bio]/Biotechnology, [SDV.BV.AP]Life Sciences [q-bio]/Vegetal Biology/Plant breeding, [SDV.BBM.GTP]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Genomics [q-bio.GN], [SDV.BC.IC]Life Sciences [q-bio]/Cellular Biology/Cell Behavior [q-bio.CB], [SDV.BDD] Life Sciences [q-bio]/Development Biology, [SDV.BC.BC] Life Sciences [q-bio]/Cellular Biology/Subcellular Processes [q-bio.SC], [SDV.BV]Life Sciences [q-bio]/Vegetal Biology, [SDV.BBM.GTP] Life Sciences [q-bio]/Biochemistry, Molecular Biology/Genomics [q-bio.GN], [SDV.BV] Life Sciences [q-bio]/Vegetal Biology, [SDV.BV.AP] Life Sciences [q-bio]/Vegetal Biology/Plant breeding, [SDV.BBM.BC]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Biochemistry [q-bio.BM], [SDV.BDD]Life Sciences [q-bio]/Development Biology, [SDV.BC] Life Sciences [q-bio]/Cellular Biology, [SDV.BBM.BC] Life Sciences [q-bio]/Biochemistry, Molecular Biology/Biochemistry [q-bio.BM], ComputingMilieux_MISCELLANEOUS, [SDV.BV.PEP] Life Sciences [q-bio]/Vegetal Biology/Phytopathology and phytopharmacy
Abstract: International audience
Published: 2013

17. The cell wall of tobacco and tomato pollen tubes contains fucosylated xyloglucan not found in somatic cells

Author: Dardelle, Flavien, Lehner, Arnaud, Bardor, Muriel, Rihouey, Christophe, Causse, Mathilde, Lerouge, Patrice, Driouich, Azeddine, Mollet, Jean-Claude, Lehner, Arnaud, Laboratoire de Glycobiologie et Matrice Extracellulaire Végétale (Glyco-MEV), Université de Rouen Normandie (UNIROUEN), Normandie Université (NU)-Normandie Université (NU), Polymères Biopolymères Surfaces (PBS), Normandie Université (NU)-Normandie Université (NU)-Centre National de la Recherche Scientifique (CNRS)-Institut national des sciences appliquées Rouen Normandie (INSA Rouen Normandie), Normandie Université (NU)-Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA), Unité de recherche Génétique et amélioration des fruits et légumes (GALF), Institut National de la Recherche Agronomique (INRA), Institut national des sciences appliquées Rouen Normandie (INSA Rouen Normandie), Institut National des Sciences Appliquées (INSA)-Normandie Université (NU)-Institut National des Sciences Appliquées (INSA)-Normandie Université (NU)-Institut de Chimie du CNRS (INC)-Institut Normand de Chimie Moléculaire Médicinale et Macromoléculaire (INC3M), Institut de Chimie du CNRS (INC)-École Nationale Supérieure d'Ingénieurs de Caen (ENSICAEN), Normandie Université (NU)-Normandie Université (NU)-Institut national des sciences appliquées Rouen Normandie (INSA Rouen Normandie), Institut National des Sciences Appliquées (INSA)-Normandie Université (NU)-Institut National des Sciences Appliquées (INSA)-Université Le Havre Normandie (ULH), Normandie Université (NU)-Université de Rouen Normandie (UNIROUEN), Normandie Université (NU)-Centre National de la Recherche Scientifique (CNRS)-Université de Caen Normandie (UNICAEN), Normandie Université (NU)-École Nationale Supérieure d'Ingénieurs de Caen (ENSICAEN), Normandie Université (NU)-Université Le Havre Normandie (ULH), Normandie Université (NU)-Centre National de la Recherche Scientifique (CNRS), and Génétique et Amélioration des Fruits et Légumes (GAFL)
Subjects: [SDV.BIO]Life Sciences [q-bio]/Biotechnology, [SDV.BBM.BM]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Molecular biology, [SDV.BC]Life Sciences [q-bio]/Cellular Biology, [SDV.BC.BC]Life Sciences [q-bio]/Cellular Biology/Subcellular Processes [q-bio.SC], [SDV.BC.IC] Life Sciences [q-bio]/Cellular Biology/Cell Behavior [q-bio.CB], [SDV.BBM.BM] Life Sciences [q-bio]/Biochemistry, Molecular Biology/Molecular biology, [SDV.BV.PEP]Life Sciences [q-bio]/Vegetal Biology/Phytopathology and phytopharmacy, [SDV.BBM.BC]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Biomolecules [q-bio.BM], [SDV.BIO] Life Sciences [q-bio]/Biotechnology, [SDV.BV.AP]Life Sciences [q-bio]/Vegetal Biology/Plant breeding, [SDV.BBM.GTP]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Genomics [q-bio.GN], [SDV.BC.IC]Life Sciences [q-bio]/Cellular Biology/Cell Behavior [q-bio.CB], [SDV.BDD] Life Sciences [q-bio]/Development Biology, [SDV.BC.BC] Life Sciences [q-bio]/Cellular Biology/Subcellular Processes [q-bio.SC], [SDV.BV]Life Sciences [q-bio]/Vegetal Biology, [SDV.BBM.GTP] Life Sciences [q-bio]/Biochemistry, Molecular Biology/Genomics [q-bio.GN], [SDV.BV] Life Sciences [q-bio]/Vegetal Biology, [SDV.BV.AP] Life Sciences [q-bio]/Vegetal Biology/Plant breeding, [SDV.BBM.BC]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Biochemistry [q-bio.BM], [SDV.BDD]Life Sciences [q-bio]/Development Biology, [SDV.BC] Life Sciences [q-bio]/Cellular Biology, [SDV.BBM.BC] Life Sciences [q-bio]/Biochemistry, Molecular Biology/Biochemistry [q-bio.BM], ComputingMilieux_MISCELLANEOUS, [SDV.BV.PEP] Life Sciences [q-bio]/Vegetal Biology/Phytopathology and phytopharmacy
Abstract: International audience
Published: 2013

18. Effect of water deficit on the cell wall of the date palm ( Phoenix dactylifera ‘Deglet nour’, Arecales) fruit during development

Author: GRIBAA, ALI, Dardelle, Flavien, Lehner, Arnaud, Rihouey, Christophe, Burel, Carole, FERCHICHI, ALI, Driouich, Azeddine, Mollet, Jean-Claude, Laboratoire de Glycobiologie et Matrice Extracellulaire Végétale (Glyco-MEV), Université de Rouen Normandie (UNIROUEN), Normandie Université (NU)-Normandie Université (NU), Polymères Biopolymères Surfaces (PBS), Institut national des sciences appliquées Rouen Normandie (INSA Rouen Normandie), Institut National des Sciences Appliquées (INSA)-Normandie Université (NU)-Institut National des Sciences Appliquées (INSA)-Normandie Université (NU)-Institut de Chimie du CNRS (INC)-Institut Normand de Chimie Moléculaire Médicinale et Macromoléculaire (INC3M), Institut de Chimie du CNRS (INC)-École Nationale Supérieure d'Ingénieurs de Caen (ENSICAEN), Normandie Université (NU)-Normandie Université (NU)-Institut national des sciences appliquées Rouen Normandie (INSA Rouen Normandie), Institut National des Sciences Appliquées (INSA)-Normandie Université (NU)-Institut National des Sciences Appliquées (INSA)-Université Le Havre Normandie (ULH), Normandie Université (NU)-Université de Rouen Normandie (UNIROUEN), Normandie Université (NU)-Centre National de la Recherche Scientifique (CNRS)-Université de Caen Normandie (UNICAEN), Normandie Université (NU)-École Nationale Supérieure d'Ingénieurs de Caen (ENSICAEN), Normandie Université (NU)-Université Le Havre Normandie (ULH), Normandie Université (NU)-Centre National de la Recherche Scientifique (CNRS), and Institut des Régions Arides (IRA)
Subjects: food and beverages, [SDV.BV.BOT]Life Sciences [q-bio]/Vegetal Biology/Botanics
Abstract: International audience; Date palm (Phoenix dactylifera) is an important crop providing a valuable nutrition source for people in many countries including the Middle East and North Africa. In recent years, the amount of rain in North Africa and especially in the Tunisian palm grove areas has dropped significantly. We investigated the growth and cell wall remodelling of fruits harvested at three key development stages from trees grown with or without water supply. During development, cell wall solubilization and remodelling was characterized by a decrease of the degree of methylesterification of pectin, an important loss of galactose content and a reduction of the branching of xylan by arabinose in irrigated condition. Water deficit had a profound effect on fruit size, pulp content, cell wall composition and remodelling. Loss of galactose content was not as important, arabinose content was significantly higher in the pectin‐enriched extracts from non‐irrigated condition, and the levels of methylesterification of pectin and O‐acetylation of xyloglucan were lower than in irrigated condition. The lower levels of hydrophobic groups (methylester and O‐acetyl) and the less intensive degradation of the hydrophilic galactan, arabinan and arabinogalactan in the cell wall may be implicated in maintaining the hydration status of the cells under water deficit.
Published: 2013

19. Deciphering the N-glycosylation pathway in microalgae using the diatom Phaeodactylum tricornutum as a model

Author: Ovide, Clément, Baïet, Bérengère, Mathieu-Rivet, Elodie, Dardelle, Flavien, Burel, Carole, Saint-Jean, Bruno, Kiefer-Meyer, Marie-Christine, Cadoret, Jean-Paul, Lerouge, Patrice, Bardor, Muriel, Lehner, Arnaud, Laboratoire de Glycobiologie et Matrice Extracellulaire Végétale (Glyco-MEV), Université de Rouen Normandie (UNIROUEN), Normandie Université (NU)-Normandie Université (NU), Physiologie et biotechnologie des Algues (PBA), and Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)
Subjects: [SDV.BIO]Life Sciences [q-bio]/Biotechnology, [SDV.BBM.BM]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Molecular biology, [SDV.BC]Life Sciences [q-bio]/Cellular Biology, [SDV.BC.BC]Life Sciences [q-bio]/Cellular Biology/Subcellular Processes [q-bio.SC], [SDV.BC.IC] Life Sciences [q-bio]/Cellular Biology/Cell Behavior [q-bio.CB], [SDV.BBM.BM] Life Sciences [q-bio]/Biochemistry, Molecular Biology/Molecular biology, [SDV.BV.PEP]Life Sciences [q-bio]/Vegetal Biology/Phytopathology and phytopharmacy, [SDV.BIO] Life Sciences [q-bio]/Biotechnology, [SDV.BV.AP]Life Sciences [q-bio]/Vegetal Biology/Plant breeding, [SDV.BBM.GTP]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Genomics [q-bio.GN], [SDV.BC.IC]Life Sciences [q-bio]/Cellular Biology/Cell Behavior [q-bio.CB], [SDV.BDD] Life Sciences [q-bio]/Development Biology, [SDV.BC.BC] Life Sciences [q-bio]/Cellular Biology/Subcellular Processes [q-bio.SC], [SDV.BV]Life Sciences [q-bio]/Vegetal Biology, [SDV.BBM.GTP] Life Sciences [q-bio]/Biochemistry, Molecular Biology/Genomics [q-bio.GN], [SDV.BV] Life Sciences [q-bio]/Vegetal Biology, [SDV.BV.AP] Life Sciences [q-bio]/Vegetal Biology/Plant breeding, [SDV.BBM.BC]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Biochemistry [q-bio.BM], [SDV.BDD]Life Sciences [q-bio]/Development Biology, [SDV.BC] Life Sciences [q-bio]/Cellular Biology, [SDV.BBM.BC] Life Sciences [q-bio]/Biochemistry, Molecular Biology/Biochemistry [q-bio.BM], ComputingMilieux_MISCELLANEOUS, [SDV.BV.PEP] Life Sciences [q-bio]/Vegetal Biology/Phytopathology and phytopharmacy
Abstract: International audience
Published: 2013

20. De la fleur à la graine : Le fabuleux destin du pollen

Author: Lehner, Arnaud, Dardelle, Flavien, Mollet , Jean Claude, Laboratoire de Glycobiologie et Matrice Extracellulaire Végétale (Glyco-MEV), Université de Rouen Normandie (UNIROUEN), Normandie Université (NU)-Normandie Université (NU), Lehner, Arnaud, Glycobiologie et transports chez les végétaux (GTCV), and Centre National de la Recherche Scientifique (CNRS)-Université de Rouen Normandie (UNIROUEN)
Subjects: [CHIM.POLY] Chemical Sciences/Polymers, [SDV.BDD.GAM] Life Sciences [q-bio]/Development Biology/Gametogenesis, [SDV.BIO]Life Sciences [q-bio]/Biotechnology, [SDV.BBM.BM]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Molecular biology, [SDV.BBM.MN]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Molecular Networks [q-bio.MN], [SDV.BC.BC]Life Sciences [q-bio]/Cellular Biology/Subcellular Processes [q-bio.SC], [SDV.BC.IC] Life Sciences [q-bio]/Cellular Biology/Cell Behavior [q-bio.CB], [SDV.BBM.BM] Life Sciences [q-bio]/Biochemistry, Molecular Biology/Molecular biology, [SDV.BIO] Life Sciences [q-bio]/Biotechnology, [SDV.BV.PEP]Life Sciences [q-bio]/Vegetal Biology/Phytopathology and phytopharmacy, [SDV.BV.AP]Life Sciences [q-bio]/Vegetal Biology/Plant breeding, [CHIM.POLY]Chemical Sciences/Polymers, [SDV.BBM.MN] Life Sciences [q-bio]/Biochemistry, Molecular Biology/Molecular Networks [q-bio.MN], [SDV.BBM.GTP]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Genomics [q-bio.GN], [SDV.BC.IC]Life Sciences [q-bio]/Cellular Biology/Cell Behavior [q-bio.CB], [SDV.BC.BC] Life Sciences [q-bio]/Cellular Biology/Subcellular Processes [q-bio.SC], [SDV.BBM.GTP] Life Sciences [q-bio]/Biochemistry, Molecular Biology/Genomics [q-bio.GN], [SDV.BV.AP] Life Sciences [q-bio]/Vegetal Biology/Plant breeding, [SDV.BBM.BC]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Biochemistry [q-bio.BM], [SDV.BBM.BC] Life Sciences [q-bio]/Biochemistry, Molecular Biology/Biochemistry [q-bio.BM], [SDV.BV.PEP] Life Sciences [q-bio]/Vegetal Biology/Phytopathology and phytopharmacy, ComputingMilieux_MISCELLANEOUS, [SDV.BDD.GAM]Life Sciences [q-bio]/Development Biology/Gametogenesis
Abstract: International audience
Published: 2012

21. Structural analysis of Chlamydomonas reinhardtii N-glycans

Author: Rivet, Elodie, Dardelle, Flavien, Le Mauff, François, Teo, Gavin, Lerouge, Patrice, Bardor, Muriel, Laboratoire de Glycobiologie et Matrice Extracellulaire Végétale (Glyco-MEV), Université de Rouen Normandie (UNIROUEN), Normandie Université (NU)-Normandie Université (NU), and Lehner, Arnaud
Subjects: [SDV.BIO]Life Sciences [q-bio]/Biotechnology, [SDV.BBM.BM]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Molecular biology, [SDV.BC.BC]Life Sciences [q-bio]/Cellular Biology/Subcellular Processes [q-bio.SC], [SDV.BC]Life Sciences [q-bio]/Cellular Biology, [SDV.BC.IC] Life Sciences [q-bio]/Cellular Biology/Cell Behavior [q-bio.CB], [SDV.BBM.BM] Life Sciences [q-bio]/Biochemistry, Molecular Biology/Molecular biology, [SDV.BIO] Life Sciences [q-bio]/Biotechnology, [SDV.BV.PEP]Life Sciences [q-bio]/Vegetal Biology/Phytopathology and phytopharmacy, [SDV.BV.AP]Life Sciences [q-bio]/Vegetal Biology/Plant breeding, [SDV.BBM.GTP]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Genomics [q-bio.GN], [SDV.BDD] Life Sciences [q-bio]/Development Biology, [SDV.BC.IC]Life Sciences [q-bio]/Cellular Biology/Cell Behavior [q-bio.CB], [SDV.BC.BC] Life Sciences [q-bio]/Cellular Biology/Subcellular Processes [q-bio.SC], [SDV.BBM.GTP] Life Sciences [q-bio]/Biochemistry, Molecular Biology/Genomics [q-bio.GN], [SDV.BV]Life Sciences [q-bio]/Vegetal Biology, [SDV.BV] Life Sciences [q-bio]/Vegetal Biology, [SDV.BV.AP] Life Sciences [q-bio]/Vegetal Biology/Plant breeding, [SDV.BBM.BC]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Biochemistry [q-bio.BM], [SDV.BC] Life Sciences [q-bio]/Cellular Biology, [SDV.BBM.BC] Life Sciences [q-bio]/Biochemistry, Molecular Biology/Biochemistry [q-bio.BM], [SDV.BDD]Life Sciences [q-bio]/Development Biology, [SDV.BV.PEP] Life Sciences [q-bio]/Vegetal Biology/Phytopathology and phytopharmacy, ComputingMilieux_MISCELLANEOUS
Abstract: International audience
Published: 2012

22. Exploring the N-glycan biosynthesis in Chlamydomonas reinhardtii

Author: Rivet-Mathieu, Elodie, Arias, Carolina, Dardelle, Flavien, Le Mauff, François, Teo, Gavin, Blanco-Rivero, Amaya, Loutelier-Bourhis, Corinne, Kiefer-Meyer, Marie-Christine, Burel, Carole, Hippler, Michael, Lerouge, Patrice, Martínez, Flor, Bardor, Muriel, Laboratoire de Glycobiologie et Matrice Extracellulaire Végétale (Glyco-MEV), Université de Rouen Normandie (UNIROUEN), Normandie Université (NU)-Normandie Université (NU), Chimie Organique et Bioorganique : Réactivité et Analyse (COBRA), Institut Normand de Chimie Moléculaire Médicinale et Macromoléculaire (INC3M), Institut de Chimie du CNRS (INC)-École Nationale Supérieure d'Ingénieurs de Caen (ENSICAEN), Normandie Université (NU)-Normandie Université (NU)-Institut national des sciences appliquées Rouen Normandie (INSA Rouen Normandie), Institut National des Sciences Appliquées (INSA)-Normandie Université (NU)-Institut National des Sciences Appliquées (INSA)-Université Le Havre Normandie (ULH), Normandie Université (NU)-Université de Rouen Normandie (UNIROUEN), Normandie Université (NU)-Centre National de la Recherche Scientifique (CNRS)-Université de Caen Normandie (UNICAEN), Normandie Université (NU)-Institut de Chimie du CNRS (INC)-École Nationale Supérieure d'Ingénieurs de Caen (ENSICAEN), Normandie Université (NU)-Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie Organique Fine (IRCOF), Institut National des Sciences Appliquées (INSA)-Normandie Université (NU)-Institut National des Sciences Appliquées (INSA)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS), and Lehner, Arnaud
Subjects: [SDV.BIO]Life Sciences [q-bio]/Biotechnology, [SDV.BBM.BM]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Molecular biology, [SDV.BC.BC]Life Sciences [q-bio]/Cellular Biology/Subcellular Processes [q-bio.SC], [SDV.BC]Life Sciences [q-bio]/Cellular Biology, [SDV.BC.IC] Life Sciences [q-bio]/Cellular Biology/Cell Behavior [q-bio.CB], [SDV.BBM.BM] Life Sciences [q-bio]/Biochemistry, Molecular Biology/Molecular biology, [SDV.BIO] Life Sciences [q-bio]/Biotechnology, [SDV.BV.PEP]Life Sciences [q-bio]/Vegetal Biology/Phytopathology and phytopharmacy, [SDV.BV.AP]Life Sciences [q-bio]/Vegetal Biology/Plant breeding, [SDV.BBM.GTP]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Genomics [q-bio.GN], [SDV.BDD] Life Sciences [q-bio]/Development Biology, [SDV.BC.IC]Life Sciences [q-bio]/Cellular Biology/Cell Behavior [q-bio.CB], [SDV.BC.BC] Life Sciences [q-bio]/Cellular Biology/Subcellular Processes [q-bio.SC], [SDV.BBM.GTP] Life Sciences [q-bio]/Biochemistry, Molecular Biology/Genomics [q-bio.GN], [SDV.BV]Life Sciences [q-bio]/Vegetal Biology, [SDV.BV] Life Sciences [q-bio]/Vegetal Biology, [SDV.BV.AP] Life Sciences [q-bio]/Vegetal Biology/Plant breeding, [SDV.BBM.BC]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Biochemistry [q-bio.BM], [SDV.BC] Life Sciences [q-bio]/Cellular Biology, [SDV.BBM.BC] Life Sciences [q-bio]/Biochemistry, Molecular Biology/Biochemistry [q-bio.BM], [SDV.BDD]Life Sciences [q-bio]/Development Biology, [SDV.BV.PEP] Life Sciences [q-bio]/Vegetal Biology/Phytopathology and phytopharmacy, ComputingMilieux_MISCELLANEOUS
Abstract: International audience
Published: 2012

23. Characterization of pollen tube xyloglucan from tobacco and tomato (Solanaceae)

Author: Dardelle, Flavien, Grare, Maxime, Lehner, Arnaud, Rihouey, Christophe, Bardor, Muriel, Lerouge, Patrice, Driouich, Azeddine, Mollet, Jean-Claude, Laboratoire de Glycobiologie et Matrice Extracellulaire Végétale (Glyco-MEV), Université de Rouen Normandie (UNIROUEN), Normandie Université (NU)-Normandie Université (NU), Polymères Biopolymères Surfaces (PBS), Institut national des sciences appliquées Rouen Normandie (INSA Rouen Normandie), Institut National des Sciences Appliquées (INSA)-Normandie Université (NU)-Institut National des Sciences Appliquées (INSA)-Normandie Université (NU)-Institut de Chimie du CNRS (INC)-Institut Normand de Chimie Moléculaire Médicinale et Macromoléculaire (INC3M), Institut de Chimie du CNRS (INC)-École Nationale Supérieure d'Ingénieurs de Caen (ENSICAEN), Normandie Université (NU)-Normandie Université (NU)-Institut national des sciences appliquées Rouen Normandie (INSA Rouen Normandie), Institut National des Sciences Appliquées (INSA)-Normandie Université (NU)-Institut National des Sciences Appliquées (INSA)-Université Le Havre Normandie (ULH), Normandie Université (NU)-Université de Rouen Normandie (UNIROUEN), Normandie Université (NU)-Centre National de la Recherche Scientifique (CNRS)-Université de Caen Normandie (UNICAEN), Normandie Université (NU)-École Nationale Supérieure d'Ingénieurs de Caen (ENSICAEN), Normandie Université (NU)-Université Le Havre Normandie (ULH), Normandie Université (NU)-Centre National de la Recherche Scientifique (CNRS), and Lehner, Arnaud
Subjects: [SDV.BIO]Life Sciences [q-bio]/Biotechnology, [SDV.BBM.BM]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Molecular biology, [SDV.BC.BC]Life Sciences [q-bio]/Cellular Biology/Subcellular Processes [q-bio.SC], [SDV.BC]Life Sciences [q-bio]/Cellular Biology, [SDV.BC.IC] Life Sciences [q-bio]/Cellular Biology/Cell Behavior [q-bio.CB], [SDV.BBM.BM] Life Sciences [q-bio]/Biochemistry, Molecular Biology/Molecular biology, [SDV.BIO] Life Sciences [q-bio]/Biotechnology, [SDV.BV.PEP]Life Sciences [q-bio]/Vegetal Biology/Phytopathology and phytopharmacy, [SDV.BV.AP]Life Sciences [q-bio]/Vegetal Biology/Plant breeding, [SDV.BBM.GTP]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Genomics [q-bio.GN], [SDV.BDD] Life Sciences [q-bio]/Development Biology, [SDV.BC.IC]Life Sciences [q-bio]/Cellular Biology/Cell Behavior [q-bio.CB], [SDV.BC.BC] Life Sciences [q-bio]/Cellular Biology/Subcellular Processes [q-bio.SC], [SDV.BBM.GTP] Life Sciences [q-bio]/Biochemistry, Molecular Biology/Genomics [q-bio.GN], [SDV.BV]Life Sciences [q-bio]/Vegetal Biology, [SDV.BV] Life Sciences [q-bio]/Vegetal Biology, [SDV.BV.AP] Life Sciences [q-bio]/Vegetal Biology/Plant breeding, [SDV.BBM.BC]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Biochemistry [q-bio.BM], [SDV.BC] Life Sciences [q-bio]/Cellular Biology, [SDV.BBM.BC] Life Sciences [q-bio]/Biochemistry, Molecular Biology/Biochemistry [q-bio.BM], [SDV.BDD]Life Sciences [q-bio]/Development Biology, [SDV.BV.PEP] Life Sciences [q-bio]/Vegetal Biology/Phytopathology and phytopharmacy, ComputingMilieux_MISCELLANEOUS
Abstract: International audience
Published: 2012

24. Exploiring the N‐glycosylation pathway in Microalgae

Author: Lerouge, Patrice, Rivet, Elodie, Burel, Carole, Dardelle, Flavien, Le Mauff, François, Baïet, Bérengère, Kiefer-Meyer, Marie-Christine, Bardor, Muriel, Laboratoire de Glycobiologie et Matrice Extracellulaire Végétale (Glyco-MEV), Université de Rouen Normandie (UNIROUEN), Normandie Université (NU)-Normandie Université (NU), and Lehner, Arnaud
Subjects: [SDV.BIO]Life Sciences [q-bio]/Biotechnology, [SDV.BBM.BM]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Molecular biology, [SDV.BC.BC]Life Sciences [q-bio]/Cellular Biology/Subcellular Processes [q-bio.SC], [SDV.BC]Life Sciences [q-bio]/Cellular Biology, [SDV.BC.IC] Life Sciences [q-bio]/Cellular Biology/Cell Behavior [q-bio.CB], [SDV.BBM.BM] Life Sciences [q-bio]/Biochemistry, Molecular Biology/Molecular biology, [SDV.BIO] Life Sciences [q-bio]/Biotechnology, [SDV.BV.PEP]Life Sciences [q-bio]/Vegetal Biology/Phytopathology and phytopharmacy, [SDV.BV.AP]Life Sciences [q-bio]/Vegetal Biology/Plant breeding, [SDV.BBM.GTP]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Genomics [q-bio.GN], [SDV.BDD] Life Sciences [q-bio]/Development Biology, [SDV.BC.IC]Life Sciences [q-bio]/Cellular Biology/Cell Behavior [q-bio.CB], [SDV.BC.BC] Life Sciences [q-bio]/Cellular Biology/Subcellular Processes [q-bio.SC], [SDV.BBM.GTP] Life Sciences [q-bio]/Biochemistry, Molecular Biology/Genomics [q-bio.GN], [SDV.BV]Life Sciences [q-bio]/Vegetal Biology, [SDV.BV] Life Sciences [q-bio]/Vegetal Biology, [SDV.BV.AP] Life Sciences [q-bio]/Vegetal Biology/Plant breeding, [SDV.BBM.BC]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Biochemistry [q-bio.BM], [SDV.BC] Life Sciences [q-bio]/Cellular Biology, [SDV.BBM.BC] Life Sciences [q-bio]/Biochemistry, Molecular Biology/Biochemistry [q-bio.BM], [SDV.BDD]Life Sciences [q-bio]/Development Biology, [SDV.BV.PEP] Life Sciences [q-bio]/Vegetal Biology/Phytopathology and phytopharmacy, ComputingMilieux_MISCELLANEOUS
Abstract: National audience
Published: 2012

25. Novel actors in pectin biosynthesis: nucleotide sugar transporters and their role in cell wall development

Author: Donas-Cofré, Daniela, Dardelle, Flavien, Mollet, Jean-Claude, Orellana, Ariel, Universidad Andrés Bello - UNAB (CHILE), Laboratoire de Glycobiologie et Matrice Extracellulaire Végétale (Glyco-MEV), Université de Rouen Normandie (UNIROUEN), Normandie Université (NU)-Normandie Université (NU), Universidad Andrés Bello [Santiago] (UNAB), and Lehner, Arnaud
Subjects: [SDV.BIO]Life Sciences [q-bio]/Biotechnology, [SDV.BBM.BM]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Molecular biology, [SDV.BC.BC]Life Sciences [q-bio]/Cellular Biology/Subcellular Processes [q-bio.SC], [SDV.BC]Life Sciences [q-bio]/Cellular Biology, [SDV.BC.IC] Life Sciences [q-bio]/Cellular Biology/Cell Behavior [q-bio.CB], [SDV.BBM.BM] Life Sciences [q-bio]/Biochemistry, Molecular Biology/Molecular biology, [SDV.BIO] Life Sciences [q-bio]/Biotechnology, [SDV.BV.PEP]Life Sciences [q-bio]/Vegetal Biology/Phytopathology and phytopharmacy, [SDV.BV.AP]Life Sciences [q-bio]/Vegetal Biology/Plant breeding, [SDV.BBM.GTP]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Genomics [q-bio.GN], [SDV.BDD] Life Sciences [q-bio]/Development Biology, [SDV.BC.IC]Life Sciences [q-bio]/Cellular Biology/Cell Behavior [q-bio.CB], [SDV.BC.BC] Life Sciences [q-bio]/Cellular Biology/Subcellular Processes [q-bio.SC], [SDV.BBM.GTP] Life Sciences [q-bio]/Biochemistry, Molecular Biology/Genomics [q-bio.GN], [SDV.BV]Life Sciences [q-bio]/Vegetal Biology, [SDV.BV] Life Sciences [q-bio]/Vegetal Biology, [SDV.BV.AP] Life Sciences [q-bio]/Vegetal Biology/Plant breeding, [SDV.BBM.BC]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Biochemistry [q-bio.BM], [SDV.BC] Life Sciences [q-bio]/Cellular Biology, [SDV.BBM.BC] Life Sciences [q-bio]/Biochemistry, Molecular Biology/Biochemistry [q-bio.BM], [SDV.BDD]Life Sciences [q-bio]/Development Biology, [SDV.BV.PEP] Life Sciences [q-bio]/Vegetal Biology/Phytopathology and phytopharmacy, ComputingMilieux_MISCELLANEOUS
Abstract: International audience
Published: 2012

26. Immuno-Glyco-Imaging in Plant Cells: Localization of Cell Wall Carbohydrate Epitopes and Their Biosynthesizing Enzymes

Author: Marie-Laure Follet-Gueye, Chevalier Laurence, Dardelle Flavien, Driouich Azeddine, Vicré-Gibouin Maïté, Bernard Sophie, Ramdani Yasmina, Mollet Jean-Claude, Plancot Barbara, Coimbra Silvia, Laboratoire de Glycobiologie et Matrice Extracellulaire Végétale (Glyco-MEV), Université de Rouen Normandie (UNIROUEN), Normandie Université (NU)-Normandie Université (NU), Groupe de physique des matériaux (GPM), Centre National de la Recherche Scientifique (CNRS)-Institut national des sciences appliquées Rouen Normandie (INSA Rouen Normandie), Institut National des Sciences Appliquées (INSA)-Normandie Université (NU)-Institut National des Sciences Appliquées (INSA)-Normandie Université (NU)-Université de Rouen Normandie (UNIROUEN), Normandie Université (NU), Universidade do Porto, Hesam Dehghani, Normandie Université (NU)-Normandie Université (NU)-Institut national des sciences appliquées Rouen Normandie (INSA Rouen Normandie), Institut National des Sciences Appliquées (INSA)-Normandie Université (NU)-Institut National des Sciences Appliquées (INSA)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche sur les Matériaux Avancés (IRMA), Université de Caen Normandie (UNICAEN), Normandie Université (NU)-Normandie Université (NU)-École Nationale Supérieure d'Ingénieurs de Caen (ENSICAEN), Normandie Université (NU)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université de Rouen Normandie (UNIROUEN), Normandie Université (NU)-Institut national des sciences appliquées Rouen Normandie (INSA Rouen Normandie), Institut National des Sciences Appliquées (INSA)-Normandie Université (NU)-Institut National des Sciences Appliquées (INSA)-Centre National de la Recherche Scientifique (CNRS)-Université de Caen Normandie (UNICAEN), Normandie Université (NU)-École Nationale Supérieure d'Ingénieurs de Caen (ENSICAEN), Normandie Université (NU)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Centre National de la Recherche Scientifique (CNRS), and Universidade do Porto = University of Porto
Subjects: 0106 biological sciences, chemistry.chemical_classification, 0303 health sciences, Carbohydrate, [SDV.BV.BOT]Life Sciences [q-bio]/Vegetal Biology/Botanics, Polysaccharide, 01 natural sciences, Epitope, Cell wall, Xyloglucan, 03 medical and health sciences, chemistry.chemical_compound, chemistry, Biochemistry, Arabinogalactan, Arabinoxylan, [SDV.BBM]Life Sciences [q-bio]/Biochemistry, Molecular Biology, Glycoprotein, 030304 developmental biology, 010606 plant biology & botany
Abstract: Plant cells are surrounded by a carbohydrate-rich compartment called the cell wall. This compartment plays a central role in growth, development, sexual reproduction and defence (Vicre et al., 2005; Mollet et al., 2007; Hematy et al., 2009; Seifert & Blaukopf, 2010). It is a carbohydrate-based structure composed mostly of polysaccharides (~90%) and glycoproteins (~10%). Polysaccharides include cellulose, pectin domains such as homogalacturonans (HG) with different levels of methylesterification, xylogalacturonan, rhamnogalacturonan-I (RG-I) and rhamnogalacturonanII (RG-II) and hemicelluloses (depending on the species, xyloglucan [XyG] or arabinoxylan) (Liepman et al., 2010), whereas cell wall proteins are either enzymatic such as glucanases (Gilbert, 2010) or nonenzymatic such as arabinogalactan proteins (AGP) (Ellis et al., 2010, Driouich & Baskin, 2008).
Published: 2012

27. Microalgae: a suitable system for the production of glycosylated biopharmaceuticals

Author: Rivet-Mathieu, Elodie, Baïet, Bérengère, Burel, Carole, Saint-Jean, Bruno, Le Mauff, François, Teo, Gavin, Kiefer-Meyer, Marie-Christine, Dardelle, Flavien, Follet-Gueye, Marie-Laure, Cadoret, Jean-Paul, Hippler, Michael, Martínez, Flor, Lerouge, Patrice, Bardor, Muriel, Laboratoire de Glycobiologie et Matrice Extracellulaire Végétale (Glyco-MEV), Université de Rouen Normandie (UNIROUEN), Normandie Université (NU)-Normandie Université (NU), Physiologie et biotechnologie des Algues (PBA), Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER), University of Münster, Universidad Autonoma de Madrid (UAM), and Lehner, Arnaud
Subjects: [SDV.BIO]Life Sciences [q-bio]/Biotechnology, [SDV.BBM.BM]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Molecular biology, [SDV.BC.BC]Life Sciences [q-bio]/Cellular Biology/Subcellular Processes [q-bio.SC], [SDV.BC]Life Sciences [q-bio]/Cellular Biology, [SDV.BC.IC] Life Sciences [q-bio]/Cellular Biology/Cell Behavior [q-bio.CB], [SDV.BBM.BM] Life Sciences [q-bio]/Biochemistry, Molecular Biology/Molecular biology, [SDV.BIO] Life Sciences [q-bio]/Biotechnology, [SDV.BV.PEP]Life Sciences [q-bio]/Vegetal Biology/Phytopathology and phytopharmacy, [SDV.BV.AP]Life Sciences [q-bio]/Vegetal Biology/Plant breeding, [SDV.BBM.GTP]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Genomics [q-bio.GN], [SDV.BDD] Life Sciences [q-bio]/Development Biology, [SDV.BC.IC]Life Sciences [q-bio]/Cellular Biology/Cell Behavior [q-bio.CB], [SDV.BC.BC] Life Sciences [q-bio]/Cellular Biology/Subcellular Processes [q-bio.SC], [SDV.BBM.GTP] Life Sciences [q-bio]/Biochemistry, Molecular Biology/Genomics [q-bio.GN], [SDV.BV]Life Sciences [q-bio]/Vegetal Biology, [SDV.BV] Life Sciences [q-bio]/Vegetal Biology, [SDV.BV.AP] Life Sciences [q-bio]/Vegetal Biology/Plant breeding, [SDV.BBM.BC]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Biochemistry [q-bio.BM], [SDV.BC] Life Sciences [q-bio]/Cellular Biology, [SDV.BBM.BC] Life Sciences [q-bio]/Biochemistry, Molecular Biology/Biochemistry [q-bio.BM], [SDV.BDD]Life Sciences [q-bio]/Development Biology, [SDV.BV.PEP] Life Sciences [q-bio]/Vegetal Biology/Phytopathology and phytopharmacy, ComputingMilieux_MISCELLANEOUS
Abstract: International audience
Published: 2011

28. Étude des mécanismes d'adhésion des tubes polliniques d'Arabidopsis thaliana par une approche de génétique chimique

Author: Dardelle, Flavien, Laboratoire de Glycobiologie et Matrice Extracellulaire Végétale (Glyco-MEV), Université de Rouen Normandie (UNIROUEN), Normandie Université (NU)-Normandie Université (NU), Université de Rouen Normandie, and Jean Claude Mollet
Subjects: [SDV.BIO]Life Sciences [q-bio]/Biotechnology, tube pollinique, [SDV.BBM.BM]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Molecular biology, [SDV.BBM.MN]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Molecular Networks [q-bio.MN], [SDV.BC]Life Sciences [q-bio]/Cellular Biology, [SDV.BC.BC]Life Sciences [q-bio]/Cellular Biology/Subcellular Processes [q-bio.SC], génétique chimique, [SDV.BV.PEP]Life Sciences [q-bio]/Vegetal Biology/Phytopathology and phytopharmacy, adhésion, [SDV.BV.AP]Life Sciences [q-bio]/Vegetal Biology/Plant breeding, paroi, composition, pollen, [SDV.BBM.GTP]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Genomics [q-bio.GN], [SDV.BC.IC]Life Sciences [q-bio]/Cellular Biology/Cell Behavior [q-bio.CB], [SDV.BV]Life Sciences [q-bio]/Vegetal Biology, [SDV.BBM.BC]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Biochemistry [q-bio.BM], [SDV.BDD.GAM]Life Sciences [q-bio]/Development Biology/Gametogenesis
Abstract: Au cours de la reproduction sexuée des plantes, la germination du pollen et la croissance des tubes polliniques sont soumises à un contrôle spatio-temporel dans les tissus femelles afin d'acheminer les gamètes mâles jusqu'aux ovules. Le tube pollinique est une cellule à croissance rapide entourée d'une matrice extracellulaire riche en polysaccharides. La croissance polarisée des tubes polliniques et leurs capacités à percevoir des signaux externes permettent de les guider à travers le tissu de transmission de l'organe femelle. Parmi ces signaux, un mécanisme de guidage par adhésion a été postulé chez le lys. Peu de travaux concernent les tubes polliniques de la plante modèle Arabidopsis thaliana. Dans le but d'identifier les interactions impliquées dans l'adhésion des tubes polliniques, nous avons entrepris 1- de caractériser les composés pariétaux des tubes et des cellules du tissu de transmission qui sont la première interface de contact entre ces cellules ; 2 - d'utiliser une approche de génétique chimique pour identifier des molécules impliquées dans l'adhésion cellulaire. Nos résultats indiquent que la paroi des tubes polliniques d'Arabidopsis se compose d'une double paroi riche en callose, pour la paroi interne et en pectines et xyloglucanes pour la paroi externe. Nous avons alors caractérisé la structure des xyloglucanes et mis en évidence que ce polymère est fortement fucosylé et O-acétylé. Cette caractéristique structurale semble être conservée parmi les espèces. L'abondance de l'arabinose provenant des chaînes latérales du rhamnogalacturonane de type 1 a été également mise en évidence. Pour étudier l'adhésion, nous avons reconstitué une matrice à partir de polysaccharides pariétaux extraits de fleurs mais aussi d'organes végétatifs de la plante. Les tubes polliniques adhèrent sur des pectines extraites à l'imidazole de fleurs et de feuilles d'Arabidopsis. Cette matrice nous a permis de constituer un test d'adhésion assez efficace et significatif pour le soumettre à une analyse de génétique chimique. Cette méthode consiste à étudier des processus biologiques en les perturbant via le criblage de petites molécules chimiques. Parmi les 258 molécules en notre possession, cinq ont montré des résultats prometteurs en réduisant significativement l'adhésion des tubes polliniques. De plus, avec certaines molécules, un effet dose-réponse a pu être mis en évidence.; During sexual plant reproduction, pollen germination and pollen tube growth require a tight spatial and temporal control while traveling through the female tissues in order to deliver properly the sperm cells to the ovules. Pollen tubes are unique, fast growing cells surrounded by a cell wall enriched in polysaccharides. They are tip-polarized cells able to perceive external signal cues to guide them through the transmitting tract of the female organs. Among these signals, guidance by adhesion has been postulated in lily. Few data are available concerning the cell wall of the pollen tube in the model plant Arabidopsis Thaliana. In order to identify the interactions implicated in pollen tube adhesion, we first characterized the cell wall components from the pollen tube and the transmitting tract tissue. Secondly, we used a chemical genetic approach to disturb cell adhesion. Our data indicate that Arabidopsis pollen tube cell wall is composed of a double cell wall, enriched in callose in the inner layer and pectins and xyloglucans in the outer layer. Characterization of the xyloglucan structure showed that it was highly fucosylated and O-acetylated. These structural features seemed to be preserved among species. In addition, the cell wall is composed of a large amount of arabinosyl residues coming from the rhamnogalacturonan-1 side chains. In order to identify molecules involved on pollen tube adhesion, we have used a chemical genetic approach. This new method uses small molecules to alter a specific biological process and identify protein(s) involved. To this end, we elaborated an artificial matrix where pollen tubes were able to adhere. The matrix constitutes of cell wall pectins extracted with imidazole from Arabidopsis flowers and vegetative organs. Then, the assay was used to screen 258 small molecules from our library. Our data showed that five out of the 258 tested molecules, were able to disturb pollen tube adhesion in a dose-dependent manner.
Published: 2011

29. Immunolocalization of cell wall carbohydrate epitopes in root border cells and border-like cells of various plant species

Author: Driouich, Azeddine, Cannesan, Marc-Antoine, Dardelle, Flavien, Durand, Caroline, Follet-Gueye, Marie-Laure, Vicré-Gibouin, Maïté, Staehelin, Andrew, Laboratoire de Glycobiologie et Matrice Extracellulaire Végétale (Glyco-MEV), Université de Rouen Normandie (UNIROUEN), Normandie Université (NU)-Normandie Université (NU), University of Colorado [Boulder], and Lehner, Arnaud
Subjects: [SDV.BIO]Life Sciences [q-bio]/Biotechnology, [SDV.BBM.BM]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Molecular biology, [SDV.BC]Life Sciences [q-bio]/Cellular Biology, [SDV.BC.BC]Life Sciences [q-bio]/Cellular Biology/Subcellular Processes [q-bio.SC], [SDV.BC.IC] Life Sciences [q-bio]/Cellular Biology/Cell Behavior [q-bio.CB], [SDV.BBM.BM] Life Sciences [q-bio]/Biochemistry, Molecular Biology/Molecular biology, [SDV.BIO] Life Sciences [q-bio]/Biotechnology, [SDV.BV.PEP]Life Sciences [q-bio]/Vegetal Biology/Phytopathology and phytopharmacy, [SDV.BV.AP]Life Sciences [q-bio]/Vegetal Biology/Plant breeding, [SDV.BBM.GTP]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Genomics [q-bio.GN], [SDV.BDD] Life Sciences [q-bio]/Development Biology, [SDV.BC.IC]Life Sciences [q-bio]/Cellular Biology/Cell Behavior [q-bio.CB], [SDV.BC.BC] Life Sciences [q-bio]/Cellular Biology/Subcellular Processes [q-bio.SC], [SDV.BBM.GTP] Life Sciences [q-bio]/Biochemistry, Molecular Biology/Genomics [q-bio.GN], [SDV.BV]Life Sciences [q-bio]/Vegetal Biology, [SDV.BV] Life Sciences [q-bio]/Vegetal Biology, [SDV.BV.AP] Life Sciences [q-bio]/Vegetal Biology/Plant breeding, [SDV.BBM.BC]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Biochemistry [q-bio.BM], [SDV.BC] Life Sciences [q-bio]/Cellular Biology, [SDV.BBM.BC] Life Sciences [q-bio]/Biochemistry, Molecular Biology/Biochemistry [q-bio.BM], [SDV.BDD]Life Sciences [q-bio]/Development Biology, [SDV.BV.PEP] Life Sciences [q-bio]/Vegetal Biology/Phytopathology and phytopharmacy, ComputingMilieux_MISCELLANEOUS
Abstract: International audience
Published: 2011

30. Effect of water deficit on the cell wall composition of date palm (Phoenix dactylifera) fruits and roots

Author: GRIBAA, ALI, Dardelle, Flavien, Rihouey, Christophe, Bernard, Sophie, Burel, Carole, Driouich, Azeddine, Mollet, Jean-Claude, Laboratoire de Glycobiologie et Matrice Extracellulaire Végétale (Glyco-MEV), Université de Rouen Normandie (UNIROUEN), Normandie Université (NU)-Normandie Université (NU), Polymères Biopolymères Surfaces (PBS), Institut national des sciences appliquées Rouen Normandie (INSA Rouen Normandie), Institut National des Sciences Appliquées (INSA)-Normandie Université (NU)-Institut National des Sciences Appliquées (INSA)-Normandie Université (NU)-Institut de Chimie du CNRS (INC)-Institut Normand de Chimie Moléculaire Médicinale et Macromoléculaire (INC3M), Institut de Chimie du CNRS (INC)-École Nationale Supérieure d'Ingénieurs de Caen (ENSICAEN), Normandie Université (NU)-Normandie Université (NU)-Institut national des sciences appliquées Rouen Normandie (INSA Rouen Normandie), Institut National des Sciences Appliquées (INSA)-Normandie Université (NU)-Institut National des Sciences Appliquées (INSA)-Université Le Havre Normandie (ULH), Normandie Université (NU)-Université de Rouen Normandie (UNIROUEN), Normandie Université (NU)-Centre National de la Recherche Scientifique (CNRS)-Université de Caen Normandie (UNICAEN), Normandie Université (NU)-École Nationale Supérieure d'Ingénieurs de Caen (ENSICAEN), Normandie Université (NU)-Université Le Havre Normandie (ULH), Normandie Université (NU)-Centre National de la Recherche Scientifique (CNRS), and Lehner, Arnaud
Subjects: [SDV.BIO]Life Sciences [q-bio]/Biotechnology, [SDV.BBM.BM]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Molecular biology, [SDV.BC.BC]Life Sciences [q-bio]/Cellular Biology/Subcellular Processes [q-bio.SC], [SDV.BC]Life Sciences [q-bio]/Cellular Biology, [SDV.BC.IC] Life Sciences [q-bio]/Cellular Biology/Cell Behavior [q-bio.CB], [SDV.BBM.BM] Life Sciences [q-bio]/Biochemistry, Molecular Biology/Molecular biology, [SDV.BIO] Life Sciences [q-bio]/Biotechnology, [SDV.BV.PEP]Life Sciences [q-bio]/Vegetal Biology/Phytopathology and phytopharmacy, [SDV.BV.AP]Life Sciences [q-bio]/Vegetal Biology/Plant breeding, [SDV.BBM.GTP]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Genomics [q-bio.GN], [SDV.BDD] Life Sciences [q-bio]/Development Biology, [SDV.BC.IC]Life Sciences [q-bio]/Cellular Biology/Cell Behavior [q-bio.CB], [SDV.BC.BC] Life Sciences [q-bio]/Cellular Biology/Subcellular Processes [q-bio.SC], [SDV.BBM.GTP] Life Sciences [q-bio]/Biochemistry, Molecular Biology/Genomics [q-bio.GN], [SDV.BV]Life Sciences [q-bio]/Vegetal Biology, [SDV.BV] Life Sciences [q-bio]/Vegetal Biology, [SDV.BV.AP] Life Sciences [q-bio]/Vegetal Biology/Plant breeding, [SDV.BBM.BC]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Biochemistry [q-bio.BM], [SDV.BC] Life Sciences [q-bio]/Cellular Biology, [SDV.BBM.BC] Life Sciences [q-bio]/Biochemistry, Molecular Biology/Biochemistry [q-bio.BM], [SDV.BDD]Life Sciences [q-bio]/Development Biology, [SDV.BV.PEP] Life Sciences [q-bio]/Vegetal Biology/Phytopathology and phytopharmacy, ComputingMilieux_MISCELLANEOUS
Abstract: International audience
Published: 2011

31. Study of A. thaliana pollen tube adhesion by a chemical genetic approach

Author: Dardelle, Flavien and Lehner, Arnaud
Subjects: [SDV.BDD.GAM] Life Sciences [q-bio]/Development Biology/Gametogenesis, tube pollinique, [SDV.BC.IC] Life Sciences [q-bio]/Cellular Biology/Cell Behavior [q-bio.CB], génétique chimique, [SDV.BBM.BM] Life Sciences [q-bio]/Biochemistry, Molecular Biology/Molecular biology, [SDV.BIO] Life Sciences [q-bio]/Biotechnology, adhésion, [SDV.BBM.MN] Life Sciences [q-bio]/Biochemistry, Molecular Biology/Molecular Networks [q-bio.MN], paroi, composition, pollen, [SDV.BC.BC] Life Sciences [q-bio]/Cellular Biology/Subcellular Processes [q-bio.SC], [SDV.BBM.GTP] Life Sciences [q-bio]/Biochemistry, Molecular Biology/Genomics [q-bio.GN], [SDV.BV] Life Sciences [q-bio]/Vegetal Biology, [SDV.BV.AP] Life Sciences [q-bio]/Vegetal Biology/Plant breeding, [SDV.BC] Life Sciences [q-bio]/Cellular Biology, [SDV.BBM.BC] Life Sciences [q-bio]/Biochemistry, Molecular Biology/Biochemistry [q-bio.BM], [SDV.BV.PEP] Life Sciences [q-bio]/Vegetal Biology/Phytopathology and phytopharmacy
Abstract: Au cours de la reproduction sexuée des plantes, la germination du pollen et la croissance des tubes polliniques sont soumises à un contrôle spatio-temporel dans les tissus femelles afin d'acheminer les gamètes mâles jusqu'aux ovules. Le tube pollinique est une cellule à croissance rapide entourée d'une matrice extracellulaire riche en polysaccharides. La croissance polarisée des tubes polliniques et leurs capacités à percevoir des signaux externes permettent de les guider à travers le tissu de transmission de l'organe femelle. Parmi ces signaux, un mécanisme de guidage par adhésion a été postulé chez le lys. Peu de travaux concernent les tubes polliniques de la plante modèle Arabidopsis thaliana. Dans le but d'identifier les interactions impliquées dans l'adhésion des tubes polliniques, nous avons entrepris 1- de caractériser les composés pariétaux des tubes et des cellules du tissu de transmission qui sont la première interface de contact entre ces cellules ; 2 - d'utiliser une approche de génétique chimique pour identifier des molécules impliquées dans l'adhésion cellulaire. Nos résultats indiquent que la paroi des tubes polliniques d'Arabidopsis se compose d'une double paroi riche en callose, pour la paroi interne et en pectines et xyloglucanes pour la paroi externe. Nous avons alors caractérisé la structure des xyloglucanes et mis en évidence que ce polymère est fortement fucosylé et O-acétylé. Cette caractéristique structurale semble être conservée parmi les espèces. L'abondance de l'arabinose provenant des chaînes latérales du rhamnogalacturonane de type 1 a été également mise en évidence. Pour étudier l'adhésion, nous avons reconstitué une matrice à partir de polysaccharides pariétaux extraits de fleurs mais aussi d'organes végétatifs de la plante. Les tubes polliniques adhèrent sur des pectines extraites à l'imidazole de fleurs et de feuilles d'Arabidopsis. Cette matrice nous a permis de constituer un test d'adhésion assez efficace et significatif pour le soumettre à une analyse de génétique chimique. Cette méthode consiste à étudier des processus biologiques en les perturbant via le criblage de petites molécules chimiques. Parmi les 258 molécules en notre possession, cinq ont montré des résultats prometteurs en réduisant significativement l'adhésion des tubes polliniques. De plus, avec certaines molécules, un effet dose-réponse a pu être mis en évidence., During sexual plant reproduction, pollen germination and pollen tube growth require a tight spatial and temporal control while traveling through the female tissues in order to deliver properly the sperm cells to the ovules. Pollen tubes are unique, fast growing cells surrounded by a cell wall enriched in polysaccharides. They are tip-polarized cells able to perceive external signal cues to guide them through the transmitting tract of the female organs. Among these signals, guidance by adhesion has been postulated in lily. Few data are available concerning the cell wall of the pollen tube in the model plant Arabidopsis Thaliana. In order to identify the interactions implicated in pollen tube adhesion, we first characterized the cell wall components from the pollen tube and the transmitting tract tissue. Secondly, we used a chemical genetic approach to disturb cell adhesion. Our data indicate that Arabidopsis pollen tube cell wall is composed of a double cell wall, enriched in callose in the inner layer and pectins and xyloglucans in the outer layer. Characterization of the xyloglucan structure showed that it was highly fucosylated and O-acetylated. These structural features seemed to be preserved among species. In addition, the cell wall is composed of a large amount of arabinosyl residues coming from the rhamnogalacturonan-1 side chains. In order to identify molecules involved on pollen tube adhesion, we have used a chemical genetic approach. This new method uses small molecules to alter a specific biological process and identify protein(s) involved. To this end, we elaborated an artificial matrix where pollen tubes were able to adhere. The matrix constitutes of cell wall pectins extracted with imidazole from Arabidopsis flowers and vegetative organs. Then, the assay was used to screen 258 small molecules from our library. Our data showed that five out of the 258 tested molecules, were able to disturb pollen tube adhesion in a dose-dependent manner.
Published: 2011

32. Les cellules bordantes de la racine chez Arabidopsis thaliana: Formation, caractérisation et rôle dans la santé de la plante

Author: Durand, Caroline, Follet-Gueye, Marie-Laure, Cannesan, Marc-Antoine, Plancot, Barbara, Dardelle, Flavien, Vicré-Gibouin, Maïté, Driouich, Azeddine, Lehner, Arnaud, Laboratoire de Glycobiologie et Matrice Extracellulaire Végétale (Glyco-MEV), Université de Rouen Normandie (UNIROUEN), and Normandie Université (NU)-Normandie Université (NU)
Subjects: [SDV.BIO]Life Sciences [q-bio]/Biotechnology, [SDV.BBM.BM]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Molecular biology, [SDV.BC.BC]Life Sciences [q-bio]/Cellular Biology/Subcellular Processes [q-bio.SC], [SDV.BC]Life Sciences [q-bio]/Cellular Biology, [SDV.BC.IC] Life Sciences [q-bio]/Cellular Biology/Cell Behavior [q-bio.CB], [SDV.BBM.BM] Life Sciences [q-bio]/Biochemistry, Molecular Biology/Molecular biology, [SDV.BV.PEP]Life Sciences [q-bio]/Vegetal Biology/Phytopathology and phytopharmacy, [SDV.BIO] Life Sciences [q-bio]/Biotechnology, [SDV.BV.AP]Life Sciences [q-bio]/Vegetal Biology/Plant breeding, [SDV.BBM.GTP]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Genomics [q-bio.GN], [SDV.BC.IC]Life Sciences [q-bio]/Cellular Biology/Cell Behavior [q-bio.CB], [SDV.BDD] Life Sciences [q-bio]/Development Biology, [SDV.BC.BC] Life Sciences [q-bio]/Cellular Biology/Subcellular Processes [q-bio.SC], [SDV.BV]Life Sciences [q-bio]/Vegetal Biology, [SDV.BBM.GTP] Life Sciences [q-bio]/Biochemistry, Molecular Biology/Genomics [q-bio.GN], [SDV.BV] Life Sciences [q-bio]/Vegetal Biology, [SDV.BV.AP] Life Sciences [q-bio]/Vegetal Biology/Plant breeding, [SDV.BBM.BC]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Biochemistry [q-bio.BM], [SDV.BDD]Life Sciences [q-bio]/Development Biology, [SDV.BC] Life Sciences [q-bio]/Cellular Biology, [SDV.BBM.BC] Life Sciences [q-bio]/Biochemistry, Molecular Biology/Biochemistry [q-bio.BM], ComputingMilieux_MISCELLANEOUS, [SDV.BV.PEP] Life Sciences [q-bio]/Vegetal Biology/Phytopathology and phytopharmacy
Abstract: National audience
Published: 2011

33. The pollen tube cell wall: a microscopic and biochemical characterization

Author: Dardelle, Flavien, Lehner, Arnaud, Ramdani, Yasmina, Bardor, Muriel, Lerouge, Patrice, Driouich, Azeddine, Mollet, Jean-Claude, Laboratoire de Glycobiologie et Matrice Extracellulaire Végétale (Glyco-MEV), Université de Rouen Normandie (UNIROUEN), Normandie Université (NU)-Normandie Université (NU), and Lehner, Arnaud
Subjects: [SDV.BIO]Life Sciences [q-bio]/Biotechnology, [SDV.BBM.BM]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Molecular biology, [SDV.BC]Life Sciences [q-bio]/Cellular Biology, [SDV.BC.BC]Life Sciences [q-bio]/Cellular Biology/Subcellular Processes [q-bio.SC], [SDV.BC.IC] Life Sciences [q-bio]/Cellular Biology/Cell Behavior [q-bio.CB], [SDV.BBM.BM] Life Sciences [q-bio]/Biochemistry, Molecular Biology/Molecular biology, [SDV.BIO] Life Sciences [q-bio]/Biotechnology, [SDV.BV.PEP]Life Sciences [q-bio]/Vegetal Biology/Phytopathology and phytopharmacy, [SDV.BV.AP]Life Sciences [q-bio]/Vegetal Biology/Plant breeding, [SDV.BBM.GTP]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Genomics [q-bio.GN], [SDV.BDD] Life Sciences [q-bio]/Development Biology, [SDV.BC.IC]Life Sciences [q-bio]/Cellular Biology/Cell Behavior [q-bio.CB], [SDV.BC.BC] Life Sciences [q-bio]/Cellular Biology/Subcellular Processes [q-bio.SC], [SDV.BBM.GTP] Life Sciences [q-bio]/Biochemistry, Molecular Biology/Genomics [q-bio.GN], [SDV.BV]Life Sciences [q-bio]/Vegetal Biology, [SDV.BV] Life Sciences [q-bio]/Vegetal Biology, [SDV.BV.AP] Life Sciences [q-bio]/Vegetal Biology/Plant breeding, [SDV.BBM.BC]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Biochemistry [q-bio.BM], [SDV.BC] Life Sciences [q-bio]/Cellular Biology, [SDV.BBM.BC] Life Sciences [q-bio]/Biochemistry, Molecular Biology/Biochemistry [q-bio.BM], [SDV.BDD]Life Sciences [q-bio]/Development Biology, [SDV.BV.PEP] Life Sciences [q-bio]/Vegetal Biology/Phytopathology and phytopharmacy, ComputingMilieux_MISCELLANEOUS
Abstract: International audience
Published: 2010

34. In silico prediction of proteins related to xyloglucan fucosyltransferases in Solanaceae genomes

Author: Lehner, Arnaud, primary, Menu-Bouaouiche, Laurence, additional, Dardelle, Flavien, additional, Le Mauff, François, additional, Driouich, Azeddine, additional, Lerouge, Patrice, additional, and Mollet, Jean-Claude, additional
Published: 2015
Full Text: View/download PDF

35. Pollen tube cell walls of wild and domesticated tomatoes contain arabinosylated and fucosylated xyloglucan

Author: Dardelle, Flavien, primary, Le Mauff, François, additional, Lehner, Arnaud, additional, Loutelier-Bourhis, Corinne, additional, Bardor, Muriel, additional, Rihouey, Christophe, additional, Causse, Mathilde, additional, Lerouge, Patrice, additional, Driouich, Azeddine, additional, and Mollet, Jean-Claude, additional
Published: 2014
Full Text: View/download PDF

36. Extracellular matrix characterization of in vitro grown Arabidopsis thaliana pollen tubes

Author: Dardelle, Flavien, Finassi, Roll Ngouala, Lehner, Arnaud, Follet-Gueye, Marie-Laure, Bardor, Muriel, Lerouge, Patrice, Driouich, Azeddine Azeddine, Mollet, Jean-Claude, Laboratoire de Glycobiologie et Matrice Extracellulaire Végétale (Glyco-MEV), Université de Rouen Normandie (UNIROUEN), Normandie Université (NU)-Normandie Université (NU), and Lehner, Arnaud
Subjects: [SDV.BIO]Life Sciences [q-bio]/Biotechnology, [SDV.BBM.BM]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Molecular biology, [SDV.BC]Life Sciences [q-bio]/Cellular Biology, [SDV.BC.BC]Life Sciences [q-bio]/Cellular Biology/Subcellular Processes [q-bio.SC], [SDV.BC.IC] Life Sciences [q-bio]/Cellular Biology/Cell Behavior [q-bio.CB], [SDV.BBM.BM] Life Sciences [q-bio]/Biochemistry, Molecular Biology/Molecular biology, [SDV.BIO] Life Sciences [q-bio]/Biotechnology, [SDV.BBM.BC]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Biomolecules [q-bio.BM], [SDV.BV.PEP]Life Sciences [q-bio]/Vegetal Biology/Phytopathology and phytopharmacy, [SDV.BV.AP]Life Sciences [q-bio]/Vegetal Biology/Plant breeding, [SDV.BBM.GTP]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Genomics [q-bio.GN], [SDV.BDD] Life Sciences [q-bio]/Development Biology, [SDV.BC.IC]Life Sciences [q-bio]/Cellular Biology/Cell Behavior [q-bio.CB], [SDV.BC.BC] Life Sciences [q-bio]/Cellular Biology/Subcellular Processes [q-bio.SC], [SDV.BBM.GTP] Life Sciences [q-bio]/Biochemistry, Molecular Biology/Genomics [q-bio.GN], [SDV.BV]Life Sciences [q-bio]/Vegetal Biology, [SDV.BV] Life Sciences [q-bio]/Vegetal Biology, [SDV.BV.AP] Life Sciences [q-bio]/Vegetal Biology/Plant breeding, [SDV.BBM.BC]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Biochemistry [q-bio.BM], [SDV.BC] Life Sciences [q-bio]/Cellular Biology, [SDV.BBM.BC] Life Sciences [q-bio]/Biochemistry, Molecular Biology/Biochemistry [q-bio.BM], [SDV.BDD]Life Sciences [q-bio]/Development Biology, [SDV.BV.PEP] Life Sciences [q-bio]/Vegetal Biology/Phytopathology and phytopharmacy, ComputingMilieux_MISCELLANEOUS
Abstract: International audience
Published: 2009

37. Role of root border cells and border-like cells in plant health and development

Author: Driouich, Azeddine, Durand, Caroline, Dardelle, Flavien, Cannesan, Marc-Antoine, Percoco, Guiseppe, Boudjeko, Thadée, Vicré-Gibouin, Maïté, Laboratoire de Glycobiologie et Matrice Extracellulaire Végétale (Glyco-MEV), Université de Rouen Normandie (UNIROUEN), Normandie Université (NU)-Normandie Université (NU), and Lehner, Arnaud
Subjects: [SDV.BIO]Life Sciences [q-bio]/Biotechnology, [SDV.BBM.BM]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Molecular biology, [SDV.BC]Life Sciences [q-bio]/Cellular Biology, [SDV.BC.BC]Life Sciences [q-bio]/Cellular Biology/Subcellular Processes [q-bio.SC], [SDV.BC.IC] Life Sciences [q-bio]/Cellular Biology/Cell Behavior [q-bio.CB], [SDV.BBM.BM] Life Sciences [q-bio]/Biochemistry, Molecular Biology/Molecular biology, [SDV.BIO] Life Sciences [q-bio]/Biotechnology, [SDV.BV.PEP]Life Sciences [q-bio]/Vegetal Biology/Phytopathology and phytopharmacy, [SDV.BV.AP]Life Sciences [q-bio]/Vegetal Biology/Plant breeding, [SDV.BBM.GTP]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Genomics [q-bio.GN], [SDV.BDD] Life Sciences [q-bio]/Development Biology, [SDV.BC.IC]Life Sciences [q-bio]/Cellular Biology/Cell Behavior [q-bio.CB], [SDV.BC.BC] Life Sciences [q-bio]/Cellular Biology/Subcellular Processes [q-bio.SC], [SDV.BBM.GTP] Life Sciences [q-bio]/Biochemistry, Molecular Biology/Genomics [q-bio.GN], [SDV.BV]Life Sciences [q-bio]/Vegetal Biology, [SDV.BV] Life Sciences [q-bio]/Vegetal Biology, [SDV.BV.AP] Life Sciences [q-bio]/Vegetal Biology/Plant breeding, [SDV.BBM.BC]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Biochemistry [q-bio.BM], [SDV.BC] Life Sciences [q-bio]/Cellular Biology, [SDV.BBM.BC] Life Sciences [q-bio]/Biochemistry, Molecular Biology/Biochemistry [q-bio.BM], [SDV.BDD]Life Sciences [q-bio]/Development Biology, [SDV.BV.PEP] Life Sciences [q-bio]/Vegetal Biology/Phytopathology and phytopharmacy, ComputingMilieux_MISCELLANEOUS
Abstract: International audience
Published: 2009

38. Génétique chimique : émergence d’une technologie nouvelle et application au contrôle de la reproduction des plantes

Author: Dardelle, Flavien, Finassi, Roll N’gouala, Lerouge, Patrice, Driouich, Azeddine, Mollet, Jean-Claude, Laboratoire de Glycobiologie et Matrice Extracellulaire Végétale (Glyco-MEV), Université de Rouen Normandie (UNIROUEN), Normandie Université (NU)-Normandie Université (NU), and Lehner, Arnaud
Subjects: [SDV.BIO]Life Sciences [q-bio]/Biotechnology, [SDV.BBM.BM]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Molecular biology, [SDV.BC]Life Sciences [q-bio]/Cellular Biology, [SDV.BC.BC]Life Sciences [q-bio]/Cellular Biology/Subcellular Processes [q-bio.SC], [SDV.BC.IC] Life Sciences [q-bio]/Cellular Biology/Cell Behavior [q-bio.CB], [SDV.BBM.BM] Life Sciences [q-bio]/Biochemistry, Molecular Biology/Molecular biology, [SDV.BIO] Life Sciences [q-bio]/Biotechnology, [SDV.BBM.BC]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Biomolecules [q-bio.BM], [SDV.BV.PEP]Life Sciences [q-bio]/Vegetal Biology/Phytopathology and phytopharmacy, [SDV.BV.AP]Life Sciences [q-bio]/Vegetal Biology/Plant breeding, [SDV.BBM.GTP]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Genomics [q-bio.GN], [SDV.BDD] Life Sciences [q-bio]/Development Biology, [SDV.BC.IC]Life Sciences [q-bio]/Cellular Biology/Cell Behavior [q-bio.CB], [SDV.BC.BC] Life Sciences [q-bio]/Cellular Biology/Subcellular Processes [q-bio.SC], [SDV.BBM.GTP] Life Sciences [q-bio]/Biochemistry, Molecular Biology/Genomics [q-bio.GN], [SDV.BV]Life Sciences [q-bio]/Vegetal Biology, [SDV.BV] Life Sciences [q-bio]/Vegetal Biology, [SDV.BV.AP] Life Sciences [q-bio]/Vegetal Biology/Plant breeding, [SDV.BBM.BC]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Biochemistry [q-bio.BM], [SDV.BC] Life Sciences [q-bio]/Cellular Biology, [SDV.BBM.BC] Life Sciences [q-bio]/Biochemistry, Molecular Biology/Biochemistry [q-bio.BM], [SDV.BDD]Life Sciences [q-bio]/Development Biology, [SDV.BV.PEP] Life Sciences [q-bio]/Vegetal Biology/Phytopathology and phytopharmacy, ComputingMilieux_MISCELLANEOUS
Abstract: International audience
Published: 2009

39. Rôle des cellules « frontières » de la racine dans la défense et la santé des plantes

Author: Driouich, Azeddine, Durand, Caroline, Dardelle, Flavien, Vicré-Gibouin, Maïté, Laboratoire de Glycobiologie et Matrice Extracellulaire Végétale (Glyco-MEV), Université de Rouen Normandie (UNIROUEN), Normandie Université (NU)-Normandie Université (NU), and Lehner, Arnaud
Subjects: [SDV.BIO]Life Sciences [q-bio]/Biotechnology, [SDV.BBM.BM]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Molecular biology, [SDV.BC]Life Sciences [q-bio]/Cellular Biology, [SDV.BC.BC]Life Sciences [q-bio]/Cellular Biology/Subcellular Processes [q-bio.SC], [SDV.BC.IC] Life Sciences [q-bio]/Cellular Biology/Cell Behavior [q-bio.CB], [SDV.BBM.BM] Life Sciences [q-bio]/Biochemistry, Molecular Biology/Molecular biology, [SDV.BIO] Life Sciences [q-bio]/Biotechnology, [SDV.BBM.BC]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Biomolecules [q-bio.BM], [SDV.BV.PEP]Life Sciences [q-bio]/Vegetal Biology/Phytopathology and phytopharmacy, [SDV.BV.AP]Life Sciences [q-bio]/Vegetal Biology/Plant breeding, [SDV.BBM.GTP]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Genomics [q-bio.GN], [SDV.BDD] Life Sciences [q-bio]/Development Biology, [SDV.BC.IC]Life Sciences [q-bio]/Cellular Biology/Cell Behavior [q-bio.CB], [SDV.BC.BC] Life Sciences [q-bio]/Cellular Biology/Subcellular Processes [q-bio.SC], [SDV.BBM.GTP] Life Sciences [q-bio]/Biochemistry, Molecular Biology/Genomics [q-bio.GN], [SDV.BV]Life Sciences [q-bio]/Vegetal Biology, [SDV.BV] Life Sciences [q-bio]/Vegetal Biology, [SDV.BV.AP] Life Sciences [q-bio]/Vegetal Biology/Plant breeding, [SDV.BBM.BC]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Biochemistry [q-bio.BM], [SDV.BC] Life Sciences [q-bio]/Cellular Biology, [SDV.BBM.BC] Life Sciences [q-bio]/Biochemistry, Molecular Biology/Biochemistry [q-bio.BM], [SDV.BDD]Life Sciences [q-bio]/Development Biology, [SDV.BV.PEP] Life Sciences [q-bio]/Vegetal Biology/Phytopathology and phytopharmacy, ComputingMilieux_MISCELLANEOUS
Abstract: International audience
Published: 2009

40. Formation and functional organization of root border cells and border-like cells

Author: Driouich, Azeddine, Durand, Caroline, Dardelle, Flavien, Cannesan, Marc-Antoine, Vicré-Gibouin, Maïté, Laboratoire de Glycobiologie et Matrice Extracellulaire Végétale (Glyco-MEV), Université de Rouen Normandie (UNIROUEN), Normandie Université (NU)-Normandie Université (NU), Normandie Université (NU), and Lehner, Arnaud
Subjects: [SDV.BIO]Life Sciences [q-bio]/Biotechnology, [SDV.BBM.BM]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Molecular biology, [SDV.BC]Life Sciences [q-bio]/Cellular Biology, [SDV.BC.BC]Life Sciences [q-bio]/Cellular Biology/Subcellular Processes [q-bio.SC], [SDV.BC.IC] Life Sciences [q-bio]/Cellular Biology/Cell Behavior [q-bio.CB], [SDV.BBM.BM] Life Sciences [q-bio]/Biochemistry, Molecular Biology/Molecular biology, [SDV.BIO] Life Sciences [q-bio]/Biotechnology, [SDV.BV.PEP]Life Sciences [q-bio]/Vegetal Biology/Phytopathology and phytopharmacy, [SDV.BV.AP]Life Sciences [q-bio]/Vegetal Biology/Plant breeding, [SDV.BBM.GTP]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Genomics [q-bio.GN], [SDV.BDD] Life Sciences [q-bio]/Development Biology, [SDV.BC.IC]Life Sciences [q-bio]/Cellular Biology/Cell Behavior [q-bio.CB], [SDV.BC.BC] Life Sciences [q-bio]/Cellular Biology/Subcellular Processes [q-bio.SC], [SDV.BBM.GTP] Life Sciences [q-bio]/Biochemistry, Molecular Biology/Genomics [q-bio.GN], [SDV.BV]Life Sciences [q-bio]/Vegetal Biology, [SDV.BV] Life Sciences [q-bio]/Vegetal Biology, [SDV.BV.AP] Life Sciences [q-bio]/Vegetal Biology/Plant breeding, [SDV.BBM.BC]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Biochemistry [q-bio.BM], [SDV.BC] Life Sciences [q-bio]/Cellular Biology, [SDV.BBM.BC] Life Sciences [q-bio]/Biochemistry, Molecular Biology/Biochemistry [q-bio.BM], [SDV.BDD]Life Sciences [q-bio]/Development Biology, [SDV.BV.PEP] Life Sciences [q-bio]/Vegetal Biology/Phytopathology and phytopharmacy, ComputingMilieux_MISCELLANEOUS
Abstract: International audience
Published: 2009

41. Exploring the N-glycosylation Pathway in Chlamydomonas reinhardtii Unravels Novel Complex Structures

Author: Mathieu-Rivet, Elodie, primary, Scholz, Martin, additional, Arias, Carolina, additional, Dardelle, Flavien, additional, Schulze, Stefan, additional, Le Mauff, François, additional, Teo, Gavin, additional, Hochmal, Ana Karina, additional, Blanco-Rivero, Amaya, additional, Loutelier-Bourhis, Corinne, additional, Kiefer-Meyer, Marie-Christine, additional, Fufezan, Christian, additional, Burel, Carole, additional, Lerouge, Patrice, additional, Martinez, Flor, additional, Bardor, Muriel, additional, and Hippler, Michael, additional
Published: 2013
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42. Cell Wall Composition, Biosynthesis and Remodeling during Pollen Tube Growth

Author: Mollet, Jean-Claude, primary, Leroux, Christelle, additional, Dardelle, Flavien, additional, and Lehner, Arnaud, additional
Published: 2013
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43. Effect of water deficit on the cell wall of the date palm (Phoenix dactylifera ‘Deglet nour’, Arecales) fruit during development

Author: GRIBAA, ALI, primary, DARDELLE, FLAVIEN, additional, LEHNER, ARNAUD, additional, RIHOUEY, CHRISTOPHE, additional, BUREL, CAROLE, additional, FERCHICHI, ALI, additional, DRIOUICH, AZEDDINE, additional, and MOLLET, JEAN‐CLAUDE, additional
Published: 2012
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44. Pectins in the cell wall ofArabidopsis thalianapollen tube and pistil

Author: Lehner, Arnaud, primary, Dardelle, Flavien, additional, Soret-Morvan, Odile, additional, Lerouge, Patrice, additional, Driouich, Azeddine, additional, and Mollet, Jean-Claude, additional
Published: 2010
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45. Pectins in the cell wall of Arabidopsis thaliana pollen tube and pistil.

Author: Lehner, Arnaud, Dardelle, Flavien, Soret-Morvan, Odile, Lerouge, Patrice, Driouich, Azeddine, and Mollet, Jean-Claude
Published: 2010
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46. Exploring the N-glycosylation Pathway in Chlamydomonas reinhardtiiUnravels Novel Complex Structures*

Author: Mathieu-Rivet, Elodie, Scholz, Martin, Arias, Carolina, Dardelle, Flavien, Schulze, Stefan, Le Mauff, François, Teo, Gavin, Hochmal, Ana Karina, Blanco-Rivero, Amaya, Loutelier-Bourhis, Corinne, Kiefer-Meyer, Marie-Christine, Fufezan, Christian, Burel, Carole, Lerouge, Patrice, Martinez, Flor, Bardor, Muriel, and Hippler, Michael
Abstract: Chlamydomonas reinhardtiiis a green unicellular eukaryotic model organism for studying relevant biological and biotechnological questions. The availability of genomic resources and the growing interest in C. reinhardtiias an emerging cell factory for the industrial production of biopharmaceuticals require an in-depth analysis of protein N-glycosylation in this organism. Accordingly, we used a comprehensive approach including genomic, glycomic, and glycoproteomic techniques to unravel the N-glycosylation pathway of C. reinhardtii.Using mass-spectrometry-based approaches, we found that both endogenous soluble and membrane-bound proteins carry predominantly oligomannosides ranging from Man-2 to Man-5. In addition, minor complex N-linked glycans were identified as being composed of partially 6-O-methylated Man-3 to Man-5 carrying one or two xylose residues. These findings were supported by results from a glycoproteomic approach that led to the identification of 86 glycoproteins. Here, a combination of in-source collision-induced dissodiation (CID) for glycan fragmentation followed by mass tag-triggered CID for peptide sequencing and PNGase F treatment of glycopeptides in the presence of 18O-labeled water in conjunction with CID mass spectrometric analyses were employed. In conclusion, our data support the notion that the biosynthesis and maturation of N-linked glycans in the endoplasmic reticulum and Golgi apparatus occur via a GnT I-independent pathway yielding novel complex N-linked glycans that maturate differently from their counterparts in land plants.
Published: 2013
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47. Pectins in the cell wall of Arabidopsis thalianapollen tube and pistil

Author: Lehner, Arnaud, Dardelle, Flavien, Soret-Morvan, Odile, Lerouge, Patrice, Driouich, Azeddine, and Mollet, Jean-Claude
Abstract: Plant sexual reproduction involves the growth of tip-polarized pollen tubes through the female tissues in order to deliver the sperm nuclei to the egg cells. Despite the importance of this crucial step, little is known about the molecular mechanisms involved in this spatial and temporal control of the tube growth. In order to study this process and to characterize the structural composition of the extracellular matrix of the male gametophyte, immunocytochemical and biochemical analyses of Arabidopsis pollen tube wall have been carried out. Results showed a well defined localization of cell wall epitopes with highly esterified homogalacturonan and arabinogalactan-protein mainly in the tip region, weakly methylesterified homogalacturonan back from the tip and xyloglucan and (1→5)-α-L-arabinan all along the tube. Here, we present complementary data regarding 1) the ultrastructure of the pollen tube cell wall and 2) the immunolocalization of homogalacturonan and arabinan epitopes in 16h-old pollen tubes and in the stigma and the transmitting tract of the female organ. Discussion regarding the pattern of the distribution of the cell wall epitopes and the possible mechanisms of cell adhesion between the pollen tubes and the female tissues is provided.
Published: 2010
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48. In silicoprediction of proteins related to xyloglucan fucosyltransferases in Solanaceae genomes

Author: Lehner, Arnaud, Menu-Bouaouiche, Laurence, Dardelle, Flavien, Le Mauff, François, Driouich, Azeddine, Lerouge, Patrice, and Mollet, Jean-Claude
Abstract: Two independent studies have shown that the cell wall of pollen tubes from tobacco and tomato species contained fucosylated xyloglucan (XyG). These findings are intriguing as many reports have shown that XyG of somatic cells of these species is not fucosylated but instead is arabinosylated. In order to produce fucosylated XyG, plants must express a functional galactoside α-2-fucosyltransferase. Here, using a bioinformatics approach, we show that several candidate genes coding for XyG fucosyltransferases are present in the genome of coffee and several Solanaceae species including tomato, tobacco, potato, eggplant and pepper. BLAST and protein alignments with the 2 well-characterized XyG fucosyltransferases from Arabidopsis thalianaand Pisum sativumrevealed that at least 6 proteins from different Solanaceae species and from coffee displayed the 3 conserved motifs required for XyG fucosyltransferase activity.
Published: 2015
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49. Effect of water deficit on the cell wall of the date palm (Phoenix dactylifera 'Deglet nour', Arecales) fruit during development.

Author: Gribaa A, Dardelle F, Lehner A, Rihouey C, Burel C, Ferchichi A, Driouich A, and Mollet JC
Subjects: Acetylation, Arecaceae growth & development, Dehydration, Esterification, Fruit metabolism, Galactans metabolism, Galactose metabolism, Glucans metabolism, Hydrophobic and Hydrophilic Interactions, Pectins metabolism, Polysaccharides metabolism, Solubility, Xylans metabolism, Arecaceae metabolism, Cell Wall metabolism, Fruit growth & development, Water metabolism
Abstract: Date palm (Phoenix dactylifera) is an important crop providing a valuable nutrition source for people in many countries including the Middle East and North Africa. In recent years, the amount of rain in North Africa and especially in the Tunisian palm grove areas has dropped significantly. We investigated the growth and cell wall remodelling of fruits harvested at three key development stages from trees grown with or without water supply. During development, cell wall solubilization and remodelling was characterized by a decrease of the degree of methylesterification of pectin, an important loss of galactose content and a reduction of the branching of xylan by arabinose in irrigated condition. Water deficit had a profound effect on fruit size, pulp content, cell wall composition and remodelling. Loss of galactose content was not as important, arabinose content was significantly higher in the pectin-enriched extracts from non-irrigated condition, and the levels of methylesterification of pectin and O-acetylation of xyloglucan were lower than in irrigated condition. The lower levels of hydrophobic groups (methylester and O-acetyl) and the less intensive degradation of the hydrophilic galactan, arabinan and arabinogalactan in the cell wall may be implicated in maintaining the hydration status of the cells under water deficit., (© 2012 Blackwell Publishing Ltd.)
Published: 2013
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