Search

Your search keyword '"Darina Hynes"' showing total 4 results
Start Over Author "Darina Hynes"
4 results on '"Darina Hynes"'

1. Dataset of KCNQ1, KCNN4, KATP channel expression and dexamethasone modulation of protein kinase signaling in airway epithelial cells

Author

Darina Hynes and Brian J. Harvey

Subjects
Computer applications to medicine. Medical informatics, R858-859.7, Science (General), Q1-390
Abstract

Dexamethasone produces anti-secretory responses in airway epithelium through the inhibition of basolateral membrane K+ channels [1–3]. We have used the human bronchial epithelial cell line 16HBE14o− to investigate the effects of dexamethasone on the expression of K+ channels and regulatory protein kinases. The data demonstrate the expression of three distinct K+ channel types – KCNQ1:KCNE3, KCNN4 and KATP which are differentially regulated by protein kinase A and protein kinase C. The data also provide evidence for rapid non-genomic actions of dexamethasone on PKC and PKA phosphorylation and their association with the various K+ channel sub-types. Biotinylation experiments provide data on the effects of dexamethasone on membrane expression of the K+ channels. Antibody co-immunoprecipitation, rtPCR and western blotting data are given for the non-genomic dexamethasone transcription-cell signaling pathway involving Gi-protein coupled receptor, PKC, adenylyl cyclase Type IV, cAMP, PKA and ERK1/2 activation. Keywords: Dexamethasone, Non-genomic, Airway epithelium, KCNQ1, KCNN4, KATP channels, Protein kinases

Published
2019
Full Text
View/download PDF

2. Dexamethasone reduces airway epithelial Cl

Author

Darina, Hynes and Brian J, Harvey

Subjects
Time Factors, Cell Membrane, Colforsin, Bronchi, Epithelial Cells, Intermediate-Conductance Calcium-Activated Potassium Channels, Dexamethasone, Cell Line, Chlorides, Gene Expression Regulation, KATP Channels, KCNQ1 Potassium Channel, Cyclic AMP, Potassium, Potassium Channel Blockers, Humans
Abstract

Basolateral membrane K

Published
2019

4. Rapid Effects of Dexamethasone on Intracellular pH and Na + /H + Exchanger Activity in Human Bronchial Epithelial Cells

Author

Valia Verriere, Jean Bousquet, Valerie Urbach, Darina Hynes, James Devaney, Sheila Faherty, Brian J. Harvey, Institut Mondor de Recherche Biomédicale (IMRB), and Institut National de la Santé et de la Recherche Médicale (INSERM)-IFR10-Université Paris-Est Créteil Val-de-Marne - Paris 12 (UPEC UP12)

Subjects
Time Factors, Anti-Inflammatory Agents, [SDV.BC.BC]Life Sciences [q-bio]/Cellular Biology/Subcellular Processes [q-bio.SC], Biochemistry, [SDV.IMM.II]Life Sciences [q-bio]/Immunology/Innate immunity, [SDV.MHEP.PSR]Life Sciences [q-bio]/Human health and pathology/Pulmonology and respiratory tract, Dexamethasone, chemistry.chemical_compound, 0302 clinical medicine, Cyclic AMP, Phosphorylation, Receptor, Cells, Cultured, ComputingMilieux_MISCELLANEOUS, Mitogen-Activated Protein Kinase 1, 0303 health sciences, Mitogen-Activated Protein Kinase 3, Hydrogen-Ion Concentration, 3. Good health, [SDV.BBM.BP]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Biophysics, Mifepristone, [SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology, Steroids, Glucocorticoid, medicine.drug, medicine.medical_specialty, Sodium-Hydrogen Exchangers, MAP Kinase Signaling System, Intracellular pH, Adenylate kinase, Bronchi, [SDV.BC]Life Sciences [q-bio]/Cellular Biology, Cycloheximide, Biology, Cell Line, 03 medical and health sciences, Internal medicine, Mineralocorticoids, medicine, [SDV.MHEP.PHY]Life Sciences [q-bio]/Human health and pathology/Tissues and Organs [q-bio.TO], Humans, [SDV.BBM.BC]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Biochemistry [q-bio.BM], Protein kinase A, Molecular Biology, Glucocorticoids, 030304 developmental biology, Epithelial Cells, Cell Biology, Molecular biology, Cyclic AMP-Dependent Protein Kinases, Sodium–hydrogen antiporter, Endocrinology, Spectrometry, Fluorescence, chemistry, Pertussis Toxin, [SDV.SP.PHARMA]Life Sciences [q-bio]/Pharmaceutical sciences/Pharmacology, Calcium, 030217 neurology & neurosurgery
Abstract

Glucocorticoids have been shown to produce rapid nongenomic responses in airway epithelia. By using an intracellular pH (pH(i)) spectrofluorescence imaging system and the NH4Cl acid-loading technique, we have shown that the synthetic glucocorticoid,dexamethasone, accelerated intracellular pH recovery after an acid load in a human bronchial epithelial cell line (16HBE14o- cells). Exposure to NH4Cl (20 mm) elicited an intracellular acidification, followed by a pH(i) recovery. Inhibition of the Na+/H+ exchanger decreased the steady-state pH(i) and antagonized the dexamethasone stimulation of pH(i) regulation. The rapid effect of dexamethasone on pH(i) was neither affected by the inhibitor of transcription, cycloheximide, nor by the classical glucocorticoid and mineralocorticoid receptors antagonists, RU486 and spironolactone, respectively. The dexamethasone effect on pH(i) regulation was reduced by inhibitors of adenylate cyclase, cAMP-dependent protein kinase and mitogenactivated protein kinase (ERK1/2). By using a PepTag assay system and Western blotting, we have shown that dexamethasone stimulated cAMP-dependent protein kinase and mitogen-activated protein kinase activities. Taken together our results provide evidence for the rapid stimulation of Na+/H+ exchange activity by glucocorticoids in bronchial epithelial cells via a nongenomic mechanism involving cAMP-dependent protein kinase and mitogen-activated protein kinase ERK1/2 pathways.

Published
2005
Full Text
View/download PDF

Catalog

Books, media, physical & digital resources
See catalog results