Search

Your search keyword '"David Echegoyen"' showing total 2 results
Start Over Author "David Echegoyen"
2 results on '"David Echegoyen"'

1. Reduced Expression of Cytoskeletal and Extracellular Matrix Genes in Human Adult Retinal Pigment Epithelium Cells Exposed to Simulated Microgravity

Author

Thomas J. Corydon, Vivek Mann, Lasse Slumstrup, Sascha Kopp, Jayashree Sahana, Anne Louise Askou, Nils E. Magnusson, David Echegoyen, Toke Bek, Alamelu Sundaresan, Stefan Riwaldt, Johann Bauer, Manfred Infanger, and Daniela Grimm

Subjects
Simulated microgravity, Retinal pigment epithelium cells, 3D growth, Spheroids, Gene expression, Random Positioning Machine, Cytoskeleton, Vascular endothelial growth factor, Physiology, QP1-981, Biochemistry, QD415-436
Abstract

Background/Aims: Microgravity (µg) has adverse effects on the eye of humans in space. The risk of visual impairment is therefore one of the leading health concerns for NASA. The impact of µg on human adult retinal epithelium (ARPE-19) cells is unknown. Methods: In this study we investigated the influence of simulated µg (s-µg; 5 and 10 days (d)), using a Random Positioning Machine (RPM), on ARPE-19 cells. We performed phase-contrast/fluorescent microscopy, qRT-PCR, Western blotting and pathway analysis. Results: Following RPM-exposure a subset of ARPE-19 cells formed multicellular spheroids (MCS), whereas the majority of the cells remained adherent (AD). After 5d, alterations of F-actin and fibronectin were observed which reverted after 10d-exposure, suggesting a time-dependent adaptation to s-µg. Gene expression analysis of 12 genes involved in cell structure, shape, adhesion, migration, and angiogenesis suggested significant changes after a 10d-RPM-exposure. 11 genes were down-regulated in AD and MCS 10d-RPM-samples compared to 1g, whereas FLK1 was up-regulated in 5d- and 10d-RPM-MCS-samples. Similarly, TIMP1 was up-regulated in 5d-RPM-samples, whereas the remaining genes were down-regulated in 5d-RPM-samples. Western blotting revealed similar changes in VEGF, β-actin, laminin and fibronectin of 5d-RPM-samples compared to 10d, whereas different alterations of β-tubulin and vimentin were observed. The pathway analysis showed complementing effects of VEGF and integrin β-1. Conclusions: These findings clearly show that s-µg induces significant alterations in the F-actin-cytoskeleton and cytoskeleton-related proteins of ARPE-19, in addition to changes in cell growth behavior and gene expression patterns involved in cell structure, growth, shape, migration, adhesion and angiogenesis.

Published
2016
Full Text
View/download PDF

2. Three-dimensional growth of human endothelial cells in an automated cell culture experiment container during the SpaceX CRS-8 ISS space mission - The SPHEROIDS project

Author

Stefan Riwaldt, Manfred Infanger, Jessica Pietsch, Thomas J. Corydon, Stefano Nebuloni, Johann Bauer, David Echegoyen, Christin Baake, Samuel Gass, Daniela Grimm, and Marcel Egli

Subjects
0301 basic medicine, Materials science, Biocompatibility, Biophysics, Cell Culture Techniques, Bioengineering, Spaceflight, Cellular viability, law.invention, Biomaterials, 03 medical and health sciences, law, International Space Station, Journal Article, Humans, Viability assay, Cells, Cultured, Cell Proliferation, Tissue Engineering, Weightlessness, Spheroid, Endothelial Cells, Equipment Design, Robotics, Space Flight, Equipment Failure Analysis, 030104 developmental biology, Simulated microgravity, Mechanics of Materials, Cell culture, Batch Cell Culture Techniques, Printing, Three-Dimensional, Ceramics and Composites, Biomedical engineering
Abstract

Human endothelial cells (ECs) were sent to the International Space Station (ISS) to determine the impact of microgravity on the formation of three-dimensional structures. For this project, an automatic experiment unit (EU) was designed allowing cell culture in space. In order to enable a safe cell culture, cell nourishment and fixation after a pre-programmed timeframe, the materials used for construction of the EUs were tested in regard to their biocompatibility. These tests revealed a high biocompatibility for all parts of the EUs, which were in contact with the cells or the medium used. Most importantly, we found polyether ether ketones for surrounding the incubation chamber, which kept cellular viability above 80% and allowed the cells to adhere as long as they were exposed to normal gravity. After assembling the EU the ECs were cultured therein, where they showed good cell viability at least for 14 days. In addition, the functionality of the automatic medium exchange, and fixation procedures were confirmed. Two days before launch, the ECs were cultured in the EUs, which were afterwards mounted on the SpaceX CRS-8 rocket. 5 and 12 days after launch the cells were fixed. Subsequent analyses revealed a scaffold-free formation of spheroids in space.

Published
2017
Full Text
View/download PDF

Catalog

Books, media, physical & digital resources
See catalog results