1. Chemical profile, antioxidant activity and cytotoxic effect of extract from leaves of Erythrochiton brasiliensis Nees & Mart. from different regions of Europe
- Author
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Aldona Adamska-Szewczyk, Barbara Rajtar, Dawid Szymczyk, Krystyna Skalicka-Woźniak, Tomasz Baj, Łukasz Świątek, Małgorzata Polz-Dacewicz, Monika Zielińska-Pisklak, and Wirginia Kukula-Koch
- Subjects
Antioxidant ,biology ,Traditional medicine ,erythrochiton brasiliensis ,Chemistry ,medicine.medical_treatment ,Erythrochiton brasiliensis ,antioxidant activity ,04 agricultural and veterinary sciences ,General Chemistry ,biology.organism_classification ,040401 food science ,0404 agricultural biotechnology ,Rutaceae ,climatic zone ,Materials Chemistry ,medicine ,rutaceae ,cytotoxicity ,Cytotoxic T cell ,Cytotoxicity ,QD1-999 - Abstract
The total phenolic content (TPC), total tannin content (TTC) and total flavonoid content (TFC) as well as the antioxidant activity and the cytotoxic effect of the extract from leaves ofErythrochiton brasiliensisNees & Mart. (Rutaceae) were evaluated. Raw material was collected in different European botanical gardens. Statistical analysis revealed a clear grouping of populations according to their climatic zone. The average TPC, TTC and TFC in tested samples were 35.92 (± 7.11) mg GAE·g–1DW, 14.98 (± 4.08) mg PyE·g–1DW and 2.92 (± 0.76) mg QuE·g–1DW, respectively. The scavenged DPPH and Trolox equivalents determined by EPR spectroscopy were 1.23–4.14 and 0.50–1.44 mmol·g–1of dry extract, respectively. Thirteen compounds (derivatives of bezoic acid acid andtrans-cinnammic acid) were identified in the samples. The flavonoid vitexin was also present as the major component in three investigated samples. Thein vitrocytotoxicity test of the extract on Vero cells provided IC50and IC10values of 175.6 and 72.5 μg·mL–1, respectively. Incubation of samples with HHV-1 infected Vero cells had no effect on the occurrence of cytopathic effect.
- Published
- 2017
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