Search

Despite an increased drive over the past two decades in Western societies to promote children's physically active play to improve their health, there are concerns that childhood has become less physically active. There are also fears that a previously naturally occurring aspect of childhood has become less authentically playful. Both trends highlight changes over time in the amount and type of play practiced by children and are often cited as consequences of generational shifts. Yet, research which analytically employs the concept of generation to connect changes to childhood with relevant social transformations is lacking. Inspired by Mannheim's conceptualisation of generations, this paper draws on life history interviews with 28 United Kingdom residents born between 1950 and 1994 to propose a fracturing of naturally occurring physical activity from childhood play. As shifts in childhood and parenting have become inextricably linked, this argument illustrates the impact of an intensification to parenting upon greater parental surveillance of increasingly organised forms of childhood physical activity at the expense of spontaneous play. Future physical activity policy should be sensitive to the social climate in which recommendations for children are made, as this places expectations upon parents due to how childhood is currently understood within neoliberal contexts. [ABSTRACT FROM AUTHOR]

The use of hyperspectral imaging in marine applications is limited, largely due to the cost-prohibitive nature of the technology and the risk of submerging such expensive electronics. Here, we examine the use of low-cost (<5000 GBP) hyperspectral imaging as a potential addition to the marine monitoring toolbox. Using coral reefs in Bermuda as a case study and a trial for the technology, data was collected across two reef morphologies, representing fringing reefs and patch reefs. Hyperspectral data of various coral species, Montastraea cavernosa, Diploria labyrinthiformis, Pseudodiploria strigosa, and Plexaurella sp., were successfully captured and analyzed, indicating the practicality and suitability of underwater hyperspectral imaging for use in coral reef assessment. The spectral data was also used to demonstrate simple spectral classification to provide values of the percentage coverage of benthic habitat types. Finally, the raw image data was used to generate digital elevation models to measure the physical structure of corals, providing another data type able to be used in reef assessments. Future improvements were also suggested regarding how to improve the spectral data captured by the technique to account for the accurate application of correction algorithms. [ABSTRACT FROM AUTHOR]

Risdiplam is an oral, survival of motor neuron 2 (SMN2) pre-mRNA splicing modifier approved for the treatment of spinal muscular atrophy (SMA). SUNFISH (NCT02908685) Part 2, a Phase 3, randomized, double-blind, placebo-controlled study, investigated the efficacy and safety of risdiplam in type 2 and non‑ambulant type 3 SMA. The primary endpoint was met: a significantly greater change from baseline in 32-item Motor Function Measure (MFM32) total score was observed with risdiplam compared with placebo at month 12. After 12 months, all participants received risdiplam while preserving initial treatment blinding. We report 24-month efficacy and safety results in this population. Month 24 exploratory endpoints included change from baseline in MFM32 and safety. MFM‑derived results were compared with an external comparator. At month 24 of risdiplam treatment, 32% of patients demonstrated improvement (a change of ≥ 3) from baseline in MFM32 total score; 58% showed stabilization (a change of ≥ 0). Compared with an external comparator, a treatment difference of 3.12 (95% confidence interval [CI] 1.67–4.57) in favor of risdiplam was observed in MFM-derived scores. Overall, gains in motor function at month 12 were maintained or improved upon at month 24. In patients initially receiving placebo, MFM32 remained stable compared with baseline (0.31 [95% CI – 0.65 to 1.28]) after 12 months of risdiplam; 16% of patients improved their score and 59% exhibited stabilization. The safety profile after 24 months was consistent with that observed after 12 months. Risdiplam over 24 months resulted in further improvement or stabilization in motor function, confirming the benefit of longer-term treatment. [ABSTRACT FROM AUTHOR]

In comparison to the natural sciences, there is a lack of empirically grounded social scientific research which addresses how people arrive at forming pleasurable interpretations of physical activity participation. Both social and natural conceptualisations of the bodily sensations evoked via physical activity involvement have also been restricted to pain and pleasure and pleasure-displeasure dualisms. Nevertheless, there is general agreement across these disciplines that pleasurable interpretations of physical activity participation encourage regular and sustained future involvement. We draw on carnal sociology to explain life history interview data from 30 varied physical activity careers to argue that corporeal experiences of being physically active are more ambiguous than existing pleasure-pain dualisms suggest. Furthermore, interpreting these ambiguous corporeal senses as pleasurable was of central importance to sustaining a prolonged physical activity career, which we argue is a carnal skill that can be learned. This skill, possessed by those interviewees with the most prolonged physical activity careers, had been acquired through becoming accustomed to the unique situated sensual ambiguities of particular physical activities, as a type of existential connoisseurship. Future research might pay more attention to the ambiguity of physical activity involvement, the carnal interpretation of which carries important consequences for the likelihood of long-term participation. [ABSTRACT FROM AUTHOR]

Intent-to-treat patients. h Patients in the placebo arm received placebo for 12 months followed by risdiplam treatment for 12 months. Baseline is the last measurement prior to the first dose of risdiplam or placebo. b Data cut-off: 30 Sep 2020. c Data cut-off: 6 Sep 2019. d Patients in the placebo arm received placebo for 12 months followed by risdiplam treatment for 12 months. 4Change in caregiver- and patient-reported SMAIS upper limb total score from baseline in patients receiving risdiplam for up to 24 months and those who previously received placebo up to study month 12. a ± 95% CI. [Extracted from the article]

Heavy nuclides like uranium and their decay products are commonly found in nuclear industries and can pose a significant health risk to humans due to their alpha-emitting properties. Traditional alpha detectors require close contact with the contaminated surface, which can be time-consuming, labour-intensive, and put personnel at risk. Remote detection is urgently needed but very challenging. To this end, a candidate detection mechanism is alpha-induced radio-luminescence. This approach uses the emission of photons from radio-ionised excited nitrogen molecules to imply the presence of alpha emitters from a distance. Herein, the use of this phenomenon to remotely image various alpha emitters with unparalleled levels of sensitivity and spatial accuracy is demonstrated. Notably, the system detected a 29 kBq Am-241 source at a distance of 3 m within 10 min. Furthermore, it demonstrated the capability to discern a 29 kBq source positioned 7 cm away from a 3 MBq source at a 2 m distance. Additionally, a 'sandwich' filter structure is described that incorporates an absorptive filter between two interference filters to enhance the ambient light rejection. The testing of the system is described in different lighting environments, including room light and inside a glovebox. This method promises safer and more efficient alpha monitoring, with applications in nuclear forensics, waste management and decommissioning. [ABSTRACT FROM AUTHOR]

Introduction: This is the first description of safety data for intravenous onasemnogene abeparvovec, the only approved systemically administered gene-replacement therapy for spinal muscular atrophy.Objective: We comprehensively assessed the safety of intravenous onasemnogene abeparvovec from preclinical studies, clinical studies, and postmarketing data.Methods: Single-dose toxicity studies were performed in neonatal mice and juvenile or neonatal cynomolgus nonhuman primates (NHPs). Data presented are from a composite of preclinical studies, seven clinical trials, and postmarketing sources (clinical trials, n = 102 patients; postmarketing surveillance, n = 665 reported adverse event [AE] cases). In clinical trials, safety was assessed through AE monitoring, vital-sign and cardiac assessments, laboratory evaluations, physical examinations, and concomitant medication use. AE reporting and available objective clinical data from postmarketing programs were evaluated.Results: The main target organs of toxicity in mice were the heart and liver. Dorsal root ganglia (DRG) inflammation was observed in NHPs. Patients exhibited no evidence of sensory neuropathy upon clinical examination. In clinical trials, 101/102 patients experienced at least one treatment-emergent AE. In total, 50 patients experienced serious AEs, including 11 considered treatment related. AEs consistent with hepatotoxicity resolved with prednisolone in clinical trials. Transient decreases in mean platelet count were detected but were without bleeding complications. Thrombotic microangiopathy (TMA) was observed in the postmarketing setting. No evidence of intracardiac thrombi was observed for NHPs or patients.Conclusions: Risks associated with onasemnogene abeparvovec can be anticipated, monitored, and managed. Hepatotoxicity events resolved with prednisolone. Thrombocytopenia was transient. TMA may require medical intervention. Important potential risks include cardiac AEs and DRG toxicity. [ABSTRACT FROM AUTHOR]

A novel method for the analysis of multivariate Raman spectroscopy data is presented. The method combines non‐negative matrix factorisation and principal component analysis, integrating the advantages and combating the disadvantages of both techniques. It involves the derivation of physically realistic spectra and the analysis of chemical and spatial trends across a sample surface. Proof of concept is demonstrated through two investigations. The first is a set of Raman spectra taken from a powder sample containing potassium sulphate, calcium carbonate and sodium sulphate. A second uses Raman data taken from an artificially corroded sample of superalloy material commonly used in gas turbine engines. This successful proof of concept for samples with unknown surface content sets the way for future development of the technique. [ABSTRACT FROM AUTHOR]

Background: Spinal muscular atrophy type 1 is a motor neuron disorder resulting in death or the need for permanent ventilation by age 2 years. We aimed to evaluate the safety and efficacy of onasemnogene abeparvovec (previously known as AVXS-101), a gene therapy delivering the survival motor neuron gene (SMN), in symptomatic patients (identified through clinical examination) with infantile-onset spinal muscular atrophy.Methods: STR1VE was an open-label, single-arm, single-dose, phase 3 trial done at 12 hospitals and universities in the USA. Eligible patients had to be younger than 6 months and have spinal muscular atrophy with biallelic SMN1 mutations (deletion or point mutations) and one or two copies of SMN2. Patients received a one-time intravenous infusion of onasemnogene abeparvovec (1·1 × 1014 vector genomes per kg) for 30-60 min. During the outpatient follow-up, patients were assessed once per week, beginning at day 7 post-infusion for 4 weeks and then once per month until the end of the study (age 18 months or early termination). Coprimary efficacy outcomes were independent sitting for 30 s or longer (Bayley-III item 26) at the 18 month of age study visit and survival (absence of death or permanent ventilation) at age 14 months. Safety was assessed through evaluation of adverse events, concomitant medication usage, physical examinations, vital sign assessments, cardiac assessments, and laboratory evaluation. Primary efficacy endpoints for the intention-to-treat population were compared with untreated infants aged 6 months or younger (n=23) with spinal muscular atrophy type 1 (biallelic deletion of SMN1 and two copies of SMN2) from the Pediatric Neuromuscular Clinical Research (PNCR) dataset. This trial is registered with ClinicalTrials.gov, NCT03306277 (completed).Findings: From Oct 24, 2017, to Nov 12, 2019, 22 patients with spinal muscular atrophy type 1 were eligible and received onasemnogene abeparvovec. 13 (59%, 97·5% CI 36-100) of 22 patients achieved functional independent sitting for 30 s or longer at the 18 month of age study visit (vs 0 of 23 patients in the untreated PNCR cohort; p<0·0001). 20 patients (91%, 79-100]) survived free from permanent ventilation at age 14 months (vs 6 [26%], 8-44; p<0·0001 in the untreated PNCR cohort). All patients who received onasemnogene abeparvovec had at least one adverse event (most common was pyrexia). The most frequently reported serious adverse events were bronchiolitis, pneumonia, respiratory distress, and respiratory syncytial virus bronchiolitis. Three serious adverse events were related or possibly related to the treatment (two patients had elevated hepatic aminotransferases, and one had hydrocephalus).Interpretation: Results from this multicentre trial build on findings from the phase 1 START study by showing safety and efficacy of commercial grade onasemnogene abeparvovec. Onasemnogene abeparvovec showed statistical superiority and clinically meaningful responses when compared with observations from the PNCR natural history cohort. The favourable benefit-risk profile shown in this study supports the use of onasemnogene abeparvovec for treatment of symptomatic patients with genetic or clinical characteristics predictive of infantile-onset spinal muscular atrophy type 1.Funding: Novartis Gene Therapies. [ABSTRACT FROM AUTHOR]

Background: High power ultra-short duration (HPUSD) ablation has been advocated to prevent esophageal injuries during atrial fibrillation (AF) ablation procedures. Prior research using the standard circular mapping catheter (CMC) has shown that ultra-short ablations may compromise lesion durability resulting in an increased need for redo procedures. The purpose of this study was to determine if HD mapping of concealed pulmonary vein (PV) connections could improve freedom from atrial fibrillation and redo procedures compared to CMC guided AF ablation. Methods: A total of 472 consecutive first time AF ablation procedure patients with at least one year of follow up were included with an average follow-up of 18 months. HPUSD AF ablation consisted of 50 W for 2-3 seconds on the posterior wall and 5-15 seconds on the anterior wall of the left atrium. Acute pulmonary vein isolation (PVI) was defined as no concealed 1) PV signals, 2) activation into PVs, or 3) voltage into PVs with no intra-procedural waiting period utilizing the HD Grid catheter versus entrance/exit block with a 30-minute wait with the circular mapping catheter. Freedom from atrial fibrillation and all atrial arrhythmias following a 90-day blanking period were assessed. Results: Acute pulmonary vein isolation was achieved in all 472 patients. HPUSD ablation using the HD Grid was associated with shorter procedure (70.2 vs 104.3 minutes, p<0.001) and fluoroscopy times (4.2 vs 15.0 minutes, p<0.001) when compared to CMC. The recurrence of any atrial arrhythmias at 1 year was 13% with HD Grid and 25% with CMC (p<0.001) with the need for redo procedures of 6% for HD Grid and 20% for CMC (p<0.001). No esophageal ulcerations/perforations were seen. No deaths, strokes, or TIAs were observed in either group. Conclusion: HPUSD AF Ablation, as guided by HD Grid mapping, may prevent esophageal injuries while at the same time improve freedom from any atrial arrhythmias and the need for redo procedures. Procedure and fluoroscopy times were also significantly decreased when compared to traditional CMC mapping. [ABSTRACT FROM AUTHOR]

Objective: To report a robust measure of the proportion of adults who do not use their hearing aids. Design: Data on hearing aid use was extracted from national household survey data, from 2004 to 2018 in Wales, UK. Study sample: A representative sample of 10,000 to 16,000 adults per year. Results: Self-reported hearing difficulty increased smoothly from 14 to 16% during the 12 years when survey administration remained unchanged. The proportion reporting that they had tried a hearing aid increased from 5 to 7% and stabilised at this level since 2011. The proportion who reported using their hearing aid most of the time increased from 47 to 52% during the 15-year period. The proportion who did not use their hearing aids at all decreased from 21 to 18% over the same period. Conclusions: In this extensively-surveyed population, approximately 20% of adults currently do not use their hearing aids at all, 30% use them some of the time and the remaining 50% most of the time. Hearing aids are valued by many, as judged by use, but there is substantial room for improvement. Inclusion of questions on use within a large-scale, regular national survey enables the collection of demonstrably reliable data. [ABSTRACT FROM AUTHOR]

Patterned deposition and 3D fabrication techniques have enabled the use of hydrogels for a number of applications including microfluidics, sensors, separations, and tissue engineering in which form fits function. Devices such as reconfigurable microvalves or implantable tissues have been created using lithography or casting techniques. Here, we present a novel open-microfluidic patterning method that utilizes surface tension forces to form hydrogel layers on top of each other, into a patterned 3D structure. We use a patterning device to form a temporary open microfluidic channel on an existing gel layer, allowing the controlled flow of unpolymerized gel in device-regions. After layer gelation and device removal, the process can be repeated iteratively to create multi-layered 3D structures. The use of open-microfluidic and surface tension-based methods to define the shape of each individual layer enables patterning to be performed with a simple pipette and with minimal dead-volume. Our method is compatible with unmodified (native) biological hydrogels, and other non-biological materials with precursor fluid properties compatible with capillary flow. With our open-microfluidic layer-by-layer fabrication method, we demonstrate the capability to build agarose, type I collagen, and polymer–peptide 3D structures featuring asymmetric designs, multiple components, overhanging features, and cell-laden regions. [ABSTRACT FROM AUTHOR]

Open microfluidic cell culture systems are powerful tools for interrogating biological mechanisms. We have previously presented a microscale cell culture system, based on spontaneous capillary flow of biocompatible hydrogels, that is integrated into a standard cell culture well plate, with flexible cell compartment geometries and easy pipet access. Here, we present two new injection molded open microfluidic devices that also easily insert into standard cell culture well plates and standard culture workflows, allowing seamless adoption by biomedical researchers. These platforms allow culture and study of soluble factor communication among multiple cell types, and the microscale dimensions are well-suited for rare primary cells. Unique advances include optimized evaporation control within the well, manufacture with reproducible and cost-effective rapid injection molding, and compatibility with sample preparation workflows for high resolution microscopy (following well-established coverslip mounting procedures). In this work, we present several use cases that highlight the usability and widespread utility of our platform including culture of limited primary testis cells from surgical patients, microscopy readouts including immunocytochemistry and single molecule fluorescence in situ hybridization (smFISH), and coculture to study interactions between adipocytes and prostate cancer cells. [ABSTRACT FROM AUTHOR]

• Cost for assimilation wetlands averaged $0.60/gallon of treatment capacity. • 2° and 3° treatment costs averaged $4.90 and $6.50/gallon, respectively. • Wetland assimilation is less sensitive to cost increases than traditional systems. In recent decades, water quality standards for wastewater treatment have become more stringent, increasing costs and energy required to reduce pollutants. Wetland assimilation is a low-cost and low-energy alternative to traditional tertiary wastewater treatment where secondarily treated and disinfected municipal effluent is discharged primarily into freshwater forested wetlands in coastal Louisiana. In this paper, costs per gallon of treatment capacity for conventional secondary and tertiary treatment were compared to those for assimilation wetlands. Cost analysis reports were used to determine costs per gallon of treatment capacity for conventional wastewater treatment facilities, including costs for conveyance between the collection system and the assimilation wetland site, and between the treatment and disposal sites if they could not be co-located. Capital and operation and maintenance costs were considered. Because all wastewater treatment plants are required to treat at least to secondary standards, costs for primary and secondary treatment were combined. If necessary, these costs were adjusted for inflation to 2017 dollars using an average inflation rate of 2.19 percent and a cumulative inflation rate of 50.84 percent. To determine costs per gallon of treatment capacity for assimilation wetlands, actual costs provided by the project engineer were used when available. To simulate the future costs of facility construction and compare the replacement costs of conventional secondary and tertiary wastewater treatment facilities and treatment wetlands in the context of energy prices, U.S. Bureau of Labor and Statistics (BLS) data for the price index for inputs to construction were used, as were the Energy Information Administration (EIA) data for the price of crude oil to model future wastewater treatment plant construction and operation costs. The cost for the Mandeville assimilation wetland included $1 million for the price of the land. Future costs of treatment facility construction and operation were modeled relative to average price of construction inputs between 1998 and 2015 using the projected price of crude oil. When treatment costs were compared among secondary, tertiary, and assimilation wetlands, mean cost for assimilation wetlands was $0.60 per gallon (>1 MGD capacity) compared to $4.90 and $6.50 per gallon for secondary and tertiary treatment, respectively. The lower total costs and energy requirements for assimilation wetlands result in lower variability in the price of construction and operation. Wetland assimilation is more economical than conventional wastewater treatment, especially compared to advanced secondary and tertiary treatment. It is likely that energy costs will increase significantly in coming decades. Because conventional secondary and tertiary treatment are energy intensive, increases in energy costs will significantly increase the costs of these treatment systems. Treatment systems that combine lower technology (e.g., oxidation ponds) secondary treatment with wetland assimilation are less likely to be impacted by rising energy costs than traditional wastewater treatment. [ABSTRACT FROM AUTHOR]

• Wetlands with fresh water inflow had higher accretion rates than areas no inflow. • Wetlands with fresh water inflow had higher productivity than areas with no inflow. • Tree ring data showed higher stem growth in wetlands with inflow than ones without. • Removal efficiencies for TN and TP averaged between 65 and 90%. • NO 3 removal efficiency averaged between 90 and 100%. The term 'assimilation wetland' has been applied to natural wetlands in Louisiana into which disinfected, secondarily treated municipal effluent is discharged with the dual purpose of improving regional water quality and enhancing vegetation productivity and soil accretion. Some municipalities began discharging treated effluent into wetlands prior to state regulations, which began in 1992. Here we review data and observations from five assimilation wetlands in the Mississippi River Delta receiving discharge of treated effluent for 26–70 years. In addition, we examine two adjacent forested wetlands, one that receives periodic Mississippi River input and one that does not. Information from these sites provides insight into how long-term nutrient input impacts coastal wetlands. Analysis of tree-ring, leaf litter, accretion, and water quality data shows that input of freshwater containing nutrients and sediments leads to enhanced wetland productivity and soil accretion via increased organic matter burial. In addition, long-term data indicate that assimilation wetlands continue to be nutrient sinks even after decades of effluent discharge, with both nitrogen and phosphorus reduced to background levels. Collectively, these data demonstrate that wetlands benefit from long-term discharge of treated municipal effluent. Properly managed wetland assimilation systems can function for long periods and lead to enhancement of degrading wetland communities in coastal Louisiana. [ABSTRACT FROM AUTHOR]

Abstract There has been considerable discussion over the past several years about the potential negative effects of nutrient loading on coastal wetland stability. In particular, there have been concerns that high nitrate concentrations can fuel denitrification that can lead to soil organic matter loss and wetland deterioration. Here we review these issues for three case studies where there have been elevated levels of dissolved inorganic nitrogen, especially nitrate, coincident with wetland deterioration. These case studies include the Breton Sound estuary that receives diverted Mississippi River water at Caernarvon LA, a freshwater assimilation wetland that receives treated municipal effluent from a wastewater treatment plant at Hammond, LA, and a tidal creek in the Plum Island Sound estuary of northeastern Massachusetts where nitrate was introduced on each flood tide during the growing season for nearly ten years. We review the physical setting, ecology and biogeochemistry of these sites and use stoichiometric calculations to estimate how much soil organic matter decomposition could be accounted for by denitrification. Results of these calculations show that denitrification rates could not have caused the observed marsh deterioration at the two LA marshes. Denitrification may play a significant role in marsh loss at the MA site, however due to the unique hydrology of this site, denitrification rates induced by very high nitrogen loading rates in concert with the hydrology of the site may play a more significant role in marsh deterioration. [ABSTRACT FROM AUTHOR]

Understanding and exploiting marine microbial biodiversity is a huge task. Integral to this is the capacity to identify and maintain exemplar taxa ex situ, so that they may be studied or utilized. This paper focuses on protists, primarily photosynthetic protists, including microalgae and macroalgae, as well as the prokaryotic cyanobacteria. It draws together the strands of activities undertaken by scientists in the fields of taxonomy, systematics and algal cultivation associated with the Scottish Association for Marine Science at Oban and its predecessors: the Scottish Marine Station, originally located on a converted lighter, The Ark, in a flooded quarry in Granton near Edinburgh, then subsequently at Millport on the Clyde; the Marine Biological Association – West Scotland at Millport; and the Scottish Marine Biological Association, founded in 1914, initially at Millport and subsequently at Dunstaffnage, Oban. The work undertaken is interwoven with the historical status and development of protistan curation over the past 130 years. The paper also examines the inter-linkages of the organization with the development of cultivation techniques and the provision of biological resources from 1914 by the then newly established Scottish Marine Biological Association to the Culture Collection of Algae and Protozoa today. Finally, we briefly outline current developments that will influence the curation and scientific exploitation of these diverse organisms in the future. [ABSTRACT FROM AUTHOR]

Flood control levees cut off the supply of sediment to Mississippi delta coastal wetlands, and contribute to putting much of the delta on a trajectory for continued submergence in the 21st century. River sediment diversions have been proposed as a method to provide a sustainable supply of sediment to the delta, but the frequency and magnitude of these diversions needs further assessment. Previous studies suggested operating river sediment diversions based on the size and frequency of natural crevasse events, which were large (>5000 m 3 /s) and infrequent (active < once a year) in the last naturally active delta. This study builds on these previous works by quantitatively assessing tradeoffs for a large, infrequent diversion into the forested wetlands of the Maurepas swamp. Land building was estimated for several diversion sizes and years inactive using a delta progradation model. A benefit-cost analysis (BCA) combined model land building results with an ecosystem service valuation and estimated costs. Results demonstrated that land building is proportional to diversion size and inversely proportional to years inactive. Because benefits were assumed to scale linearly with land gain, and costs increase with diversion size, there are disadvantages to operating large diversions less often, compared to smaller diversions more often for the immediate project area. Literature suggests that infrequent operation would provide additional gains (through increased benefits and reduced ecosystem service costs) to the broader Lake Maurepas-Pontchartrain-Borgne ecosystem. Future research should incorporate these additional effects into this type of BCA, to see if this changes the outcome for large, infrequent diversions. [ABSTRACT FROM AUTHOR]

We assessed whether the sex ratio of nestling Marsh Tits (Poecile palustris) varied with habitat fragmentation by sampling broods from two British woodland areas with contrasting patch size, and comparing the data with published results from an extensive forest in Poland. We found no evidence for manipulation of offspring sex ratios, supporting findings from Poland, and suggesting that this trait may be typical of the species. The results are important in helping to understand the origin of biased sex ratios observed among adult Marsh Tits in the declining population in Britain. [ABSTRACT FROM AUTHOR]

Algal-bacterial co-cultures, rather than cultures of algae alone, are regarded as having the potential to enhance productivity and stability in industrial algal cultivation. As with other inocula in biotechnology, to avoid loss of production strains, it is important to develop preservation methods for the long-term storage of these cultures, and one of the most commonly used approaches is cryopreservation. However, whilst there are many reports of cryopreserved xenic algal cultures, little work has been reported on the intentional preservation of both algae and beneficial bacteria in xenic cultures. Instead, studies have focused on the development of methods to conserve the algal strain(s) present, or to avoid overgrowth of bacteria in xenic isolates during the post-thaw recovery phase. Here, we have established a co-cryopreservation method for the long-term storage of both partners in a unialgal-bacterial co-culture. This is an artificial model mutualism between the alga Lobomonas rostrata and the bacterium Mesorhizobium loti, which provides vitamin B12 (cobalamin) to the alga in return for photosynthate. Using a Planer Kryo 360 controlled-rate cooler, post-thaw viability (PTV) values of 72% were obtained for the co-culture, compared to 91% for the axenic alga. The cultures were successfully revived after 6 months storage in liquid nitrogen, and continued to exhibit mutualism. Furthermore, the alga could be cryopreserved with non-symbiotic bacteria, without bacterial overgrowth occurring. It was also possible to use less controllable passive freezer chambers to cryopreserve the co-cultures, although the PTV was lower. Finally, we demonstrated that an optimised cryopreservation method may be used to prevent the overgrowth potential of non-symbiotic, adventitious bacteria in both axenic and co-cultures of L. rostrata after thawing. [ABSTRACT FROM AUTHOR]

Over 25% of Mississippi River delta plain (MRDP) wetlands were lost over the past century. There is currently a major effort to restore the MRDP focused on a 50-year time horizon, a period during which the energy system and climate will change dramatically. We used a calibrated MRDP marsh elevation model to assess the costs of hydraulic dredging to sustain wetlands from 2016 to 2066 and 2016 to 2100 under a range of scenarios for sea level rise, energy price, and management regimes. We developed a subroutine to simulate dredging costs based on the price of crude oil and a project efficiency factor. Crude oil prices were projected using forecasts from global energy models. The costs to sustain marsh between 2016 and 2100 changed from $128,000/ha in the no change scenario to ~$1,010,000/ha in the worst-case scenario for sea level rise and energy price, an ~ 8-fold increase. Increasing suspended sediment concentrations, which is possible using managed river diversions, raised created marsh lifespan and decreased long term dredging costs. Created marsh lifespan changed nonlinearly with dredging fill elevation and suspended sediment level. Cost effectiveness of marsh creation and nourishment can be optimized by adjusting dredging fill elevation to the local sediment regime. Regardless of management scenario, sustaining the MRDP with hydraulic dredging suffered declining returns on investment due to the convergence of energy and climate trends. Marsh creation will likely become unaffordable in the mid to late 21st century, especially if river sediment diversions are not constructed before 2030. We recommend that environmental managers take into consideration coupled energy and climate scenarios for long-term risk assessments and adjust restoration goals accordingly. [ABSTRACT FROM AUTHOR]

In October 1993 and January 1994, two large floods with peak discharge of 9800 and 10,980 m 3 /s and total suspended solid transport of 10.7 × 10 6 and 9.7 × 10 6 tons, respectively, occurred on the Rhône River. Both floods led to multiple levee breeches in the Northern part of the delta resulting in the introduction of 131 × 10 6 and 54.9 × 10 6 m 3 of river water, respectively. In both cases, the flood water drained to the southern lagoons and was partly pumped directly back to the Rhône or to the sea. Most of the 390,000 tons of sediment introduced remained in the Northern inundated area with accretion ranging from 70 mm near the breaches to 4 mm 6–8 km away. This last value is close to the mean accretion value (3.7 mm) inferred from the water budget and the estimation of the total quantity of sediment introduced in the flooded area. In a small area near the mouth of the Rhône river still receiving natural overflow from the river, total deposition during both floods was as high as 10 cm. The Rhône delta is facing an uncertain future with projected sea-level rise. The results of this study show that large introductions of river water can help sustain the delta in the face of climate change. Controlled introductions of river water using riverside closable structures, as in being done in other deltas, could be done in a way that delivers water and sediments to the places where it is needed most and at the same time protect important infrastructure. [ABSTRACT FROM AUTHOR]

Neuromuscular disorders are a group of conditions that can result in weakness of skeletal muscles. Examples include fatal diseases such as amyotrophic lateral sclerosis and conditions associated with high morbidity such as myopathies (muscle diseases). Many of these disorders are known to have abnormal protein folding and protein aggregates. Thus, easy to apply methods for the detection of such changes may prove useful diagnostic biomarkers. Raman spectroscopy has shown early promise in the detection of muscle pathology in neuromuscular disorders and is well suited to characterising the conformational profiles relating to protein secondary structure. In this work, we assess if Raman spectroscopy can detect differences in protein structure in muscle in the setting of neuromuscular disease. We utilise in vivo Raman spectroscopy measurements from preclinical models of amyotrophic lateral sclerosis and the myopathy Duchenne muscular dystrophy, together with ex vivo measurements of human muscle samples from individuals with and without myopathy. Using quantitative conformation profiling and matrix factorisation we demonstrate that quantitative 'conformational fingerprinting' can be used to identify changes in protein folding in muscle. Notably, myopathic conditions in both preclinical models and human samples manifested a significant reduction in α-helix structures, with concomitant increases in β-sheet and, to a lesser extent, nonregular configurations. Spectral patterns derived through non-negative matrix factorisation were able to identify myopathy with a high accuracy (79% in mouse, 78% in human tissue). This work demonstrates the potential of conformational fingerprinting as an interpretable biomarker for neuromuscular disorders. [ABSTRACT FROM AUTHOR]

Background: The natural history of spinal muscular atrophy (SMA) is well understood, with progressive muscle weakness resulting in declines in function. The development of contractures is common and negatively impacts function. Clinically, joint hypermobility (JH) is observed but is poorly described, and its relationship with function is unknown. Methods: Lower-limb ROM (range of motion) assessments of extension and flexion at the hip, knee, and ankle were performed. ROMs exceeding the published norms were included in the analysis. The functional assessments performed included the six-minute walk test (6 MWT) and the Hammersmith Functional Motor Scale—Expanded (HFMSE). Results: Of the 143 participants, 86% (n = 123) had at least one ROM measure that was hypermobile, and 22% (n = 32) had three or more. The HFMSE scores were inversely correlated with hip extension JH (r = −0.60, p = 0.21; n = 6) and positively correlated with knee flexion JH (r = 0.24, p = 0.02, n = 89). There was a moderate, inverse relationship between the 6 MWT distance and ankle plantar flexion JH (r = −0.73, p = 0.002; n = 15). Conclusions: JH was identified in nearly all participants in at least one joint in this study. Hip extension, knee flexion and ankle plantar flexion JH was associated with function. A further understanding of the trajectory of lower-limb joint ROM is needed to improve future rehabilitation strategies. [ABSTRACT FROM AUTHOR]

Authors evaluated the performance of a commercially available next-generation sequencing assay kit; this was based on genomic content from Illumina's TruSight™ Oncology 500 research assay that identifies BRCA variants and proprietary algorithms licensed from Myriad and, with additional genomic content, measures the homologous recombination deficiency (HRD) genomic instability score (GIS) in tumor tissue (TSO 500 HRD assay). Data from the TSO 500 HRD assay were compared with data from the Myriad MyChoice®CDx PLUS assay (Myriad assay). Prevalence rates for overall HRD status and BRCA mutations (a deleterious or suspected deleterious BRCA1 or BRCA2 mutation or both) and assay agreement rates for HRD GIS and BRCA analysis were assessed in ovarian tumor samples. Pearson correlations of the continuous HRD GIS and analytic sensitivity and specificity were evaluated. The prevalence of overall HRD positivity was 51.2% (TSO 500 HRD assay) versus 49.2% (Myriad assay) and the prevalence of BRCA mutations was 27.6% (TSO 500 HRD assay) versus 25.5% (Myriad assay). After post-processing optimization, concordance of the HRD GIS was 0.980 in all samples and 0.976 in the non-BRCA mutation cohort; the area under the receiver operating characteristic curve was 0.995 and 0.992, respectively. Comparison between the Illumina and Myriad assays showed that overall HRD status, the individual components of BRCA analysis, and HRD GIS detection results were highly concordant (>93%), suggesting the TSO 500 HRD assay will approach the analytical accuracy of the FDA-approved Myriad assay. • HRD status was evaluated using two tests (Illumina TSO 500 HRD assay and Myriad MyChoice®CDx PLUS assay). • Overall HRD, BRCA mutation, and HRD GIS status detection showed >93% agreement between the tests. • Prevalence of HRD-positive and BRCA mutation status were similar between the tests. • HRD GIS was strongly correlated between the two tests. [ABSTRACT FROM AUTHOR]

A correction is presented to the article "Clinical Trial and Postmarketing Safety of Onasemnogene Abeparvovec Therapy."

The Culture Collection of Freshwater Microalgae (CCMA-UFSCar, Coleção de Culturas de Microalgas de Água-Doce), based at the Universidade Federal de São Carlos, plays an important role in underpinning Brazilian microalgal research, providing biological materials, substrates and training personnel for a large proportion of the past and current projects in this area. However, recent efforts to expand the culture catalogue/holdings have reached a ceiling because of the logistical practicality of maintaining actively growing cultures. In order to reduce the costs associated with the maintenance regime of cultures, efforts were initiated on the establishment of a cryopreserved biobank for long term maintenance of cultures, thus minimizing the efforts associated with handling of material, as successfully frozen cultures, in theory, could be maintained effectively indefinitely, with the advantage of the stability of cells characteristics. Initial tests were performed on 93 strains including exemplar taxa across the different taxonomic groups in the collection catalogue. The highest levels of success were achieved for the smaller taxa, such as the small green algae; while for the larger and more complex organisms limited to no success was obtained. For the strains tested, over 70% had positive good levels of post-thaw viability and regenerated phenotypically normal cultures. [ABSTRACT FROM AUTHOR]

Sand transport to the Mississippi River Delta (MRD) remains sufficient to build wetlands in shallow, sheltered coastal bays fed by engineered diversions on the Mississippi River (MR) and its Atchafalaya River (AR) distributary. But suspended mud (silt & clay) flux to the coast has dropped from a mean of 390 Mt y −1 in the early 1950s, to 100 Mt y −1 since 1970. This fine-grained sediment travels deeper into receiving estuarine basins and plays a critical role in sustaining existing marshes. Virtually all of the 300 Mt y −1 of missing mud once flowed from the Missouri River (MOR) Basin before nearly 100 dams were built as part of the Pick-Sloan water development project. About 100 Mt y −1 is now intercepted by main-stem Upper MOR dams closed in 1953. But the remaining 200 Mt y −1 is trapped by impoundments built on tributaries to the Lower MOR in the 1950s and 1960s. Sediment flux during the post-dam high MOR discharge years of 1973, 1993 and 2011 approached pre-dam levels when tributaries to the Lower MOR, including the Platte and Kansas Rivers, contributed to flood flows. West bank tributaries drain a vast, arid part of the Great Plains, while those entering from the east bank traverse the lowlands of the MOR floodplain. Both provinces are dominated by highly erodible loess soils. Staunching the continued decline in MR fine-grained sediment flux has assumed greater importance now that engineered diversions are being built to reconnect the Lowermost MR to the MRD. Tributary dam bypassing in the Lower MOR basin could increase mud supply to the MRD by 100–200 Mt y −1 within 1–2 decades. Such emergency measures to save the MRD are compatible with objectives of the Missouri River Restoration and Platte River Recovery Programs to restore MOR riparian habitat for endangered species. Rapid mobilization to shunt fine-grained sediments past as many as 50 Lower MOR tributary dams in several U.S. states will undoubtedly require as much regional coordination and funding in the 21st century as the monumental effort it took to build the dams in the last century. [ABSTRACT FROM AUTHOR]

Deltas are among the most productive and economically important of global ecosystems but unfortunately they are also among the most threatened by human activities. Here we discuss deltas and human impact, several approaches to defining deltaic sustainability and present a ranking of sustainability. Delta sustainability must be considered within the context of global biophysical and socioeconomic constraints that include thermodynamic limitations, scale and embeddedness, and constraints at the level of the biosphere/geosphere. The development, functioning, and sustainability of deltas are the result of external and internal inputs of energy and materials, such as sediments and nutrients, that include delta lobe development, channel switching, crevasse formation, river floods, storms and associated waves and storm surges, and tides and other ocean currents. Modern deltas developed over the past several thousand years with relatively stable global mean sea level, predictable material inputs from drainage basins and the sea, and as extremely open systems. Human activity has changed these conditions to make deltas less sustainable, in that they are unable to persist through time structurally or functionally. Deltaic sustainability can be considered from geomorphic, ecological, and economic perspectives, with functional processes at these three levels being highly interactive. Changes in this functioning can lead to either enhanced or diminished sustainability, but most changes have been detrimental. There is a growing understanding that the trajectories of global environmental change and cost of energy will make achieving delta sustainability more challenging and limit options for management. Several delta types are identified in terms of sustainability including those in arid regions, those with high and low energy-intensive management systems, deltas below sea level, tropical deltas, and Arctic deltas. Representative deltas are ranked on a sustainability range. Success in sustainable delta management will depend on utilizing natural delta functioning and an ecological engineering approach. [ABSTRACT FROM AUTHOR]

Currently the Mississippi delta stands as a highly degraded and threatened coastal ecosystem having lost about 25% of coastal wetlands during the 20th century. To address this problem, a $50 billion, 50-year restoration program is underway. A central component of this program is reintroduction of river water back into the deltaic plain to mimic natural functioning of the delta. However, opposition to diversions has developed based on a number of perceived threats. These include over-freshening of coastal estuaries, displacement of fisheries, perceived water quality problems, and assertions that nutrients in river water leads to wetland deterioration. In addition, growing climate impacts and increasing scarcity and cost of energy will make coastal restoration more challenging and limit restoration options. We address these issues in the context of an analysis of natural and artificial diversions, crevasse splays, and small sub-delta lobes. We suggest that episodic large diversions and crevasses (>5000 m 3 s −1 ) can build land quickly while having transient impacts on the estuarine system. Small diversions (<200 m 3 s −1 ) that are more or less continuously operated build land slowly and can lead to over-freshening and water level stress. We use land building rates for different sized diversions and impacts of large periodic inputs of river water to coastal systems in the Mississippi delta to conclude that high discharge diversions operated episodically will lead to rapid coastal restoration and alleviate concerns about diversions. Single diversion events have deposited sediments up to 40 cm in depth over areas up to 130–180 km 2 . This approach should have broad applicability to deltas globally. [ABSTRACT FROM AUTHOR]

The United States Congress’ Southern Congressional Delegation promulgated the Declaration of Constitutional Principles, popularly known as the Southern Manifesto, on March 12, 1956. The Southern Manifesto was the South’s primary means to effectively delay implementation of public school desegregation as ordered by the United States Supreme Court decision,Brown v. Board of Topeka, Kansas(1954; as cited in Day, 2014). This essay places the desegregation of American public school system within the larger context of the time period in which it transpired, and explains how racial disparity in public education was perpetuated after the Jim Crow caste system was dismantled in the 1960s. Ironically, while de jure desegregation of American public schools was effectively accomplished by the early 1970s under the administration of President Richard M. Nixon, government spending during the era after World War II, perpetuated racial and economic disparity in America’s public schools that prevails up to the present day. [ABSTRACT FROM PUBLISHER]

In a 2011 Anecdote department article in the Annals, Alex McKenzie provided an excellent account of the events between 1974 and 1976 leading up to INWG 96, a proposed internetwork transport protocol. McKenzie's anecdote focused on the events in INWG (International Network Working Group), which this article shows were a small part of a much larger debate that was going on outside. The author places the INWG discussions in this wider context to better understand the technical points and implications, their ultimate impact, and the paradigm shift that threatened established business models. [ABSTRACT FROM PUBLISHER]

Background TMPRSS2:ERG (T2:ERG) and prostate cancer antigen 3 (PCA3) are the most advanced urine-based prostate cancer (PCa) early detection biomarkers. Objective Validate logistic regression models, termed Mi-Prostate Score (MiPS), that incorporate serum prostate-specific antigen (PSA; or the multivariate Prostate Cancer Prevention Trial risk calculator version 1.0 [PCPTrc]) and urine T2:ERG and PCA3 scores for predicting PCa and high-grade PCa on biopsy. Design, setting, and participants T2:ERG and PCA3 scores were generated using clinical-grade transcription-mediated amplification assays. Pretrained MiPS models were applied to a validation cohort of whole urine samples prospectively collected after digital rectal examination from 1244 men presenting for biopsy. Outcome measurements and statistical analysis Area under the curve (AUC) was used to compare the performance of serum PSA (or the PCPTrc) alone and MiPS models. Decision curve analysis (DCA) was used to assess clinical benefit. Results and limitations Among informative validation cohort samples ( n = 1225 [98%], 80% from patients presenting for initial biopsy), models incorporating T2:ERG had significantly greater AUC than PSA (or PCPTrc) for predicting PCa (PSA: 0.693 vs 0.585; PCPTrc: 0.718 vs 0.639; both p < 0.001) or high-grade (Gleason score >6) PCa on biopsy (PSA: 0.729 vs 0.651, p < 0.001; PCPTrc: 0.754 vs 0.707, p = 0.006). MiPS models incorporating T2:ERG score had significantly greater AUC (all p < 0.001) than models incorporating only PCA3 plus PSA (or PCPTrc or high-grade cancer PCPTrc [PCPThg]). DCA demonstrated net benefit of the MiPS_PCPTrc (or MiPS_PCPThg) model compared with the PCPTrc (or PCPThg) across relevant threshold probabilities. Conclusions Incorporating urine T2:ERG and PCA3 scores improves the performance of serum PSA (or PCPTrc) for predicting PCa and high-grade PCa on biopsy. Patient summary Incorporation of two prostate cancer (PCa)-specific biomarkers ( TMPRSS2:ERG and PCA3 ) measured in the urine improved on serum prostate-specific antigen (or a multivariate risk calculator) for predicting the presence of PCa and high-grade PCa on biopsy. A combined test, Mi-Prostate Score, uses models validated in this study and is clinically available to provide individualized risk estimates. [ABSTRACT FROM AUTHOR]

The East Joyce Wetlands (EJW) bordering northwest Lake Pontchartrain have a long history of human induced changes, such as leveeing of the Mississippi River that eliminated almost all riverine input to the area and segmentation of the east and west Joyce wetlands by the construction of a railroad, U.S. highway 51, and Interstate 55. Dredged drainage canals and associated spoil banks channel upland runoff around the wetlands. The deep canal associated with I-55 causes both rapid short-circuiting of freshwater runoff to Lake Maurepas and saltwater intrusion from Lake Pontchartrain. Increasing soil salinity has caused wide-spread loss of forested wetlands in the areas. Recently, the discharge of secondarily treated municipal effluent into the northeastern EJW as part of the Hammond wetland assimilation project has focused attention on the area (i.e., Bodker et al., 2015 ). In response, we carried out a number of studies at the Hammond Assimilation Wetlands (HAW) detailed in Shaffer et al. (2015) , as well as a series of hydrological measurements and modeling detailed here. These data show that drainage under the railroad was minimal and most flow through the wetlands was to the southeast. Water levels in the HAW were highly variable prior to the beginning of effluent discharge in 2006, with relatively high mean water levels that did not increase substantially from 2007 through summer 2009 despite the addition of municipal effluent. Following effluent addition, surface water levels lacked the variability of the pre-discharge period and mean water levels were about 20 cm higher from late 2009 until 2014 due to high rainfall in 2009, 2012, and 2013 and high effluent inflow due to significant infiltration into the city collection system. Historical net watershed inputs averaged 2.69 cm yr −1 if this volume of water were spread over the 4 km 2 area immediately south of the effluent distribution system, compared to 0.38 cm yr −1 for the effluent and 0.13 cm yr −1 for direct precipitation. Salinity records from five sites in the EJW showed a gradient of increasing salinity from north to south and strong seasonality, averaging 1.9–2.1 PSU near the lake to 0.4–0.6 PSU in the northwestern EJW. Peak salinities were 4.6–5.1 PSU near the lake and 1.8 PSU in northwestern EJW. There was also a significant decrease in salinity over time. Salinity was lower beginning in 2010 coinciding with the closure of the Mississippi River Gulf Outlet, high precipitation in the fall and winter of 2009, and in 2012 and 2013, and continuing operation of the assimilation system. Proposed plans to alternate effluent discharge between east and west Joyce wetlands should increase surface water depth variability as seen prior to effluent discharge and minimize salinity intrusion in both areas. [ABSTRACT FROM AUTHOR]

We demonstrate the first use of a multifibre Raman probe that fits inside the bore of a hypodermic needle. A Raman probe containing multiple collection fibres provides improved signal collection efficiency in biological samples compared with a previous two-fibre design. Furthermore, probe performance (signal-to-noise ratios) compared favourably with the performance achieved in previous Raman microscope experiments able to distinguish between benign lymph nodes, primary malignancies in lymph nodes and secondary malignancies in lymph nodes. The experimental measurements presented here give an indication of the sampling volume of the Raman needle probe in lymphoid tissues. Liquid tissue phantoms were used that contained scattering medium encompassing a range of scattering properties similar to those of a variety of tissue types, including lymph node tissues. To validate the appropriateness of the phantoms, the sampling depth of the probe was also measured in excised lymph node tissue. More than 50 % of Raman photons collected were found to originate from between the tip of the needle and a depth of 500 μm into the tissue. The needle probe presented here achieves spectral quality comparable to that in numerous studies previously demonstrating Raman disease discrimination. It is expected that this approach could achieve targeted subcutaneous tissue measurements and be viable for use for the in vivo Raman diagnostics of solid organs located within a few centimetres below the skin's surface. [Figure not available: see fulltext.] [ABSTRACT FROM AUTHOR]