1. Development of Anti-Human B Blood Group Monoclonal Antibodies Suitable as a Blood Typing Reagent
- Author
-
Adolfo Jamett, De Ioannes Ae, Juica F, Barros S, Aguayo J, Tzichinovsky S, and María Inés Becker
- Subjects
medicine.drug_class ,Immunology ,Antibody Affinity ,Biology ,Monoclonal antibody ,ABO Blood-Group System ,Serology ,Mice ,Antigen ,Antibody Specificity ,Isoantibodies ,Genetics ,medicine ,Animals ,Humans ,Avidity ,Typing ,Mice, Inbred BALB C ,Hemagglutination assay ,Antibodies, Monoclonal ,Hemagglutination Tests ,Virology ,Molecular biology ,Titer ,Blood Grouping and Crossmatching ,biology.protein ,Female ,Indicators and Reagents ,Antibody - Abstract
An improved procedure for the generation of high-avidity anti-human B blood group monoclonal antibodies (MAbs) was developed. One of them, termed 7A1-2, showed excellent qualities of titer, avidity, and intensity required for use as human B blood typing reagent. Hemagglutination inhibition studies with monosaccharides and oligosaccharides were carried out to determine the specificity of the MAb 7A1-2. These studies indicate that the antibody reacts with the immunodominant region of the antigen which is known to confer the serologic specificity of this blood group.
- Published
- 1994