1. An African Salmonella Typhimurium ST313 sublineage with extensive drug-resistance and signatures of host adaptation
- Author
-
Derek Pickard, Simon Clare, Katherine Harcourt, Eva Heinz, Jan Jacobs, Dauly Ngbonda, Elizabeth J. Klemm, Nicole E. Wheeler, Emily A. Lees, Marie-France Phoba, Stijn Deborggraeve, Tessa De Block, Sushmita Sridhar, Barbara Barbé, Koen Vandelannoote, Dadi Falay, Octavie Lunguya, Lisette Mbuyi Kalonji, Eve L. Coomber, Laura Maria Francisca Kuijpers, Gordon Dougan, Sandra Van Puyvelde, Van Puyvelde, Sandra [0000-0001-8434-5732], Heinz, Eva [0000-0003-4413-3756], Coomber, Eve L [0000-0002-7093-2263], Lees, Emily A [0000-0003-0229-7509], Wheeler, Nicole E [0000-0003-4599-9164], Klemm, Elizabeth J [0000-0002-2175-3752], Kuijpers, Laura [0000-0002-4480-7872], Jacobs, Jan [0000-0002-8657-6777], Deborggraeve, Stijn [0000-0003-1501-5355], Apollo - University of Cambridge Repository, Coomber, Eve L. [0000-0002-7093-2263], Lees, Emily A. [0000-0003-0229-7509], Wheeler, Nicole E. [0000-0003-4599-9164], and Klemm, Elizabeth J. [0000-0002-2175-3752]
- Subjects
0301 basic medicine ,Salmonella typhimurium ,Salmonella ,THP-1 Cells ,631/326/325/2482 ,General Physics and Astronomy ,Drug resistance ,Azithromycin ,medicine.disease_cause ,706/134 ,wc_269 ,Antimicrobial resistance ,ANNOTATION ,38/70 ,DISEASE ,Mice ,Plasmid ,Ciprofloxacin ,Bacterial genetics ,Drug Resistance, Multiple, Bacterial ,NONTYPHOIDAL SALMONELLA ,EPIDEMIOLOGY ,129 ,lcsh:Science ,Phylogeny ,Developing world ,Multidisciplinary ,biology ,SPECTRUM BETA-LACTAMASE ,Adaptation, Physiological ,3. Good health ,Anti-Bacterial Agents ,Multidisciplinary Sciences ,Salmonella enterica ,INFECTIONS ,Democratic Republic of the Congo ,Science & Technology - Other Topics ,64/60 ,Host adaptation ,Engineering sciences. Technology ,medicine.drug ,Plasmids ,631/114/739 ,141 ,Science ,NUCLEOTIDE ,030106 microbiology ,13/106 ,45/23 ,wa_395 ,Microbial Sensitivity Tests ,SEQUENCE ,General Biochemistry, Genetics and Molecular Biology ,Article ,Microbiology ,qw_45 ,Cell Line ,03 medical and health sciences ,medicine ,Animals ,Humans ,qw_131 ,Whole genome sequencing ,Science & Technology ,45 ,Whole Genome Sequencing ,General Chemistry ,ENTERICA ,biology.organism_classification ,Multiple drug resistance ,Mice, Inbred C57BL ,030104 developmental biology ,692/699/255/1318 ,DISCOVERY ,Biofilms ,631/326/22/1434 ,lcsh:Q ,Bacterial infection - Abstract
Bloodstream infections by Salmonella enterica serovar Typhimurium constitute a major health burden in sub-Saharan Africa (SSA). These invasive non-typhoidal (iNTS) infections are dominated by isolates of the antibiotic resistance-associated sequence type (ST) 313. Here, we report emergence of ST313 sublineage II.1 in the Democratic Republic of the Congo. Sublineage II.1 exhibits extensive drug resistance, involving a combination of multidrug resistance, extended spectrum β-lactamase production and azithromycin resistance. ST313 lineage II.1 isolates harbour an IncHI2 plasmid we name pSTm-ST313-II.1, with one isolate also exhibiting decreased ciprofloxacin susceptibility. Whole genome sequencing reveals that ST313 II.1 isolates have accumulated genetic signatures potentially associated with altered pathogenicity and host adaptation, related to changes observed in biofilm formation and metabolic capacity. Sublineage II.1 emerged at the beginning of the 21st century and is involved in on-going outbreaks. Our data provide evidence of further evolution within the ST313 clade associated with iNTS in SSA., Invasive non-typhoidal Salmonella (iNTS) infections are dominated by antibiotic resistant isolates of the sequence type (ST) 313. Here, the authors identify the ST313 sublineage II.1 in the Democratic Republic of the Congo exhibiting extensive drug resistance and genetic signatures potentially associated with host adaptation.
- Published
- 2019