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Primary failure of eruption (PFE) is a rare, nonsyndromic condition in which non-ankylosed teeth fail to erupt either fully or partially due to malfunction of the eruption mechanism. Three young patients are presented in this article with features consistent with a diagnosis of PFE. The features of this condition are described together with different modalities of treatment planning for such patients. The following is a discussion about zygoma implants as a new approach to treatment for patients with PFE requiring full-mouth rehabilitation. [ABSTRACT FROM AUTHOR]

Purpose: To determine the caries status in children’s deciduous teeth and examine the influence of family oral health behaviours on the caries status. Materials and Methods: This cross-sectional study included 329 children aged 3–6 years in rural Heishanzui Township, Hebei Province, China, and used a completely random sampling method. These children underwent physical and oral health examinations. The questionnaires were given to the parents and caregivers of the examined children. Results: The prevalence of caries in the deciduous dentition among children aged 3–6 years was 80.55%, with a dmft index of 4.93. Children in the caries group ate sweets, chocolates, and carbonated drinks more frequently than did children in the caries-free group (p < 0.05). Children in the caries-free group brushed their teeth more frequently, with parents helping their children brush, more often than did those in the caries-affected group (p < 0.05). The level of parental education and annual household income also had statistically significant effects on the prevalence of caries in the two groups (p < 0.05). Logistic regression analysis revealed that the frequency of eating sweets was a risk factor for caries in deciduous teeth (odds ratio = 2.20; p < 0.05). Conclusion: The prevalence of caries in deciduous teeth among children aged 3–6 years in rural Heishanzui Township was high. Compared to children in the caries-affected group, the families and children in the caries-free group had better oral hygiene behaviours. Moreover, the frequency of eating sweets was shown to be a risk factor for caries in deciduous teeth in children aged 3–6 years. [ABSTRACT FROM AUTHOR]

Blood and urine are historically the most frequent matrices used for measuring chemical levels in human biomonitoring studies. As biomonitoring programs are refreshed, consideration of specific priority substances and specific population targets provide opportunities for inclusion of alternative non- or minimally invasive matrices. This review describes methods used in health risk assessment to characterize exposure and risk based upon biomarkers from noninvasive matrices other than urine or blood, including human milk, hair, fingernails, toenails, exhaled breath, deciduous teeth, sweat, semen, meconium, and feces. Illustrative examples of these methods relevant to chemical management are provided. This review suggests that, although these alternative noninvasive biomarkers are not frequently used in human health risk assessment at present, these biomarkers may prove useful in (1) characterizing exposure and health risk in vulnerable populations, (2) cumulative risk assessments, and (3) community-based risk assessments, depending upon the substance of concern. To incorporate alternative noninvasive biomarkers into human health risk assessments with confidence, more research is needed to improve our knowledge of the relationships between external dose, internal dose, and biologic consequent effects in matrices other than blood and urine. [ABSTRACT FROM AUTHOR]

This empirical study assessed the potential of developing a machine-learning model to identify children and adolescents with poor oral health using only self-reported survey data. Such a model could enable scalable and cost-effective screening and targeted interventions, optimizing limited resources to improve oral health outcomes. To train and test the model, we used data from 2,133 students attending schools in a Portuguese municipality. Poor oral health (the dependent variable) was defined as having a Decayed, Missing, and Filled Teeth index for deciduous teeth (dmft) or permanent teeth (DMFT) above expert-defined thresholds (dmft/DMFT ≥ 3 or 4). The survey provided information about the students' oral health habits, knowledge, beliefs, and food and physical activity habits, which served as independent variables. Logistic regression models with variables selected through low-variance filtering and recursive feature elimination outperformed various others trained with complex machine learning algorithms based on precision@k metric, outperforming also random selection and expert rule-based models in identifying students with poor oral health. The proposed models are inherently explainable, broadly applicable, which given the context, could compensate their lower performance (Area Under the Curve = 0.64—0.70) compared to similar approaches and models. This study is one of the few in oral health care that includes bias auditing of classification models. The audit surfaced potential biases related to demographic factors such as age and social assistance status. Addressing these biases without significantly compromising model performance remains a challenge. The results confirm the feasibility of survey-based machine learning models for identifying individuals with poor oral health, but further validation of this approach and pilot testing in field trials are necessary. [ABSTRACT FROM AUTHOR]

Background: Traumatic dental injuries (TDIs) in early childhood exert a negative impact on the quality of life of children and their families. However, there is no evidence from cohort studies on which to base prevention strategies at the beginning of life, when children are entirely dependent upon supervision. The aim of the present study was to estimate the incidence of TDI in the first year of life and explore risk factors in a birth cohort followed up in three major cities in different regions of Brazil. Methods: A birth cohort study was conducted with children in the cities of Porto Alegre, Salvador, and Manaus. Socioeconomic, demographic, and anthropometric characteristics were collected at baseline. Behavioral characteristics were collected when the children were six and 12 months of age. After the first year of life, clinical examinations were performed by trained, calibrated dentists for the assessment of TDI (Andreasen's criteria). Poisson regression models were used to estimate associations between the independent variables and TDI, with the calculation of crude and adjusted relative risk (RR) and respective 95% confidence intervals (CI). Results: The incidence of TDI in the first year of life (range: 12 to 16 months) was 18.1% (95% CI: 13.6–22.6%). The multivariable analysis revealed a significantly greater risk of TDI in children from non-nuclear families (RR = 1.83; 95% CI: 1.11-3.00) as well as those whose mothers had a higher level of schooling (RR = 2.42; 95% CI: 1.04–5.63) and when tooth eruption began up to six months of age (RR = 2.30; 95% CI: 1.34–3.95). Being the first-born child was a protective factor (RR = 0.48; 95% CI: 0.27–0.87) for the occurrence of the outcome. Conclusion: The high incidence of TDI in the first year of life indicates the need for primary prevention strategies with an emphasis on active supervision in the first months of life. Risk factors associated with TDI related to the family, mother and child suggest pathways for the planning of such strategies. [ABSTRACT FROM AUTHOR]

Background: Inflammation in the periapical area of primary teeth can affect the development and eruption of permanent teeth. In an asymptomatic course, they are detected accidentally during routine examinations. In such cases, they often reach significant dimensions and cause irreversible changes. Methods: This report presents the case of a 9-year-old boy with a radicular cyst in the mandible, resulting in the displacement of both premolar tooth buds. To address the inflammation, facilitate the proper eruption of the impacted teeth, and prevent adjacent teeth from shifting, the primary molars were extracted. Marsupialization was performed under general anesthesia, followed by the fabrication and placement of an obturator. Results: Two months after treatment, the displaced tooth buds began aligning along the natural eruption pathway. By the 8-month follow-up, the premolars had successfully erupted into their proper positions in the oral cavity. Conclusions: Marsupialization combined with the use of an obturator is an effective first-line treatment for radicular cysts in pediatric patients, offering a conservative approach that promotes natural tooth eruption while preserving the surrounding dentition. [ABSTRACT FROM AUTHOR]

Background: Cleft lip and/or palate is the most common congenital orofacial deformity, affecting 1/800 births. A thorough review of the literature has shown that children with cleft have poorer oral hygiene and dental health than other children, with higher levels of caries in both temporary and permanent teeth and poorer periodontal health. Cleft patients are treated by a multidisciplinary team that aims to provide comprehensive care from pre- or post-natal diagnosis to early adulthood and the end of growth. We aim to assess, through a single-center, prospective, randomized and controlled clinical trial conducted at the Nantes Cleft Centre, the impact of an enhanced dental program on oral hygiene and dental health in cleft patients. Methods: Patients aged 3 to 15 years with unilateral or bilateral cleft lip and/or palate will be offered to participate in this study. After acceptance, patients will be randomly assigned to 2 groups: test group or control group. For the control group, there will be no change in the patient's care, and they will continue to see their surgeon on an annual basis. For the test group, a 30–45-min consultation will be added to the annual check-up. During this visit, patients and their parents will receive oral and written oral hygiene instructions to improve oral hygiene knowledge and brushing technique. If necessary, dental treatment will be provided at the same appointment or at a later date. In addition, patients will be contacted via telemedicine every 2 months to increase motivation, and an additional dental appointment will be scheduled at 6 months. Discussion: This is a single-center study which, if conclusive, could lead to a paradigm shift in the dental care pathway for cleft patients. Trial registration: NCT05867862 submitted 2023–07-05 https://clinicaltrials.gov/study/NCT05867862?locStr=Nantes,%20France&country=France&state=Pays%20de%20la%20Loire&city=Nantes&distance=50&cond=CLEFT%20LIP&rank=1--Trial Sponsor Nantes University Hospital. Trial status: 8th Version date 2023 May 05; recruitment began in June 2023 and will be completed in June 2024. [ABSTRACT FROM AUTHOR]

Background: Endodontic treatment aims in the preservation of extremely carious primary teeth. For root canal therapy to be successful, root canals must be properly prepared and effectively irrigated.Therefore, it is necessary to select the proper root canal disinfection method to preserve the primary tooth. Objective: This research was carried out to compare non-thermal pressure plasma (NTPP), diode laser, propolis, and chlorhexidine (CHX) efficacy for disinfection of deciduous anterior root canals contaminated with Enterococcus Faecalis (E. faecalis) after sterilization by gamma radiation. Methods: In this study, forty extracted single-rooted primary anterior teeth were used. All teeth were cleaned, disinfected, and stored till use. Gaining access was provided till reaching the orifices of canals, all pulp tissue debris was removed, and root canals of all teeth were prepared. Standardized 8 mm root length was obtained through crown decronation below the cemento-enamel junction. Samples were sterilized by gamma radiation then the bacterial suspension was inoculated inside root canals. Specimens divided into four main groups; ten samples each group. Group I: Samples irrigated with chlorhexidine. Group II: Samples treated with diode laser. Group III: Samples irrigated with Ethanolic extract of propolis. Group IV: Samples treated with NTPP. Results: A significant difference was found between values measured before and after four irrigation types (p < 0.001) for CHX, Diode Laser, NTPP and (P = 0.035) for Propolis. The highest values of colony reduction measured before and after irrigation were for NTPP (4.06 ± 0.88). Maximum reduction in colony-forming units was recorded in the NTPP group (98.79%), while the lowest reduction in colony-forming units was recorded in Propolis group (81.99%). Conclusion: All tested methods (CHX, NTPP, Diode laser and Propolis) decreased colony count, with the highest reduction noted in group treated by NTPP and the least reduction noticed in Propolis treated group. [ABSTRACT FROM AUTHOR]

Backgound: Preformed metal crowns (PMCs) and zirconia crowns (ZCs) are commonly used for the treatment of primary molar caries. However, there have been no reports on factors influencing parental awareness, willingness to use these treatments, and the associated discomfort after crown placement in children.This study is the first to report factors influencing preformed crowns, providing a reference for the prevention and treatment of deciduous carious teeth. Methods: Parents whose children's posterior caries were restored with preformed crowns at Shenzhen Children's Hospital from October 2021 to October 2023 were recruited. A questionnaire gathered data on multiple variables, including basic information about the participants, their oral health behaviors, and their attitudes toward oral health. Results: Approximately two-thirds of the surveyed parents were aware of preformed metal crowns (PMCs) (67.8%), whereas over half were hardly aware of prefabricated zirconia crowns (ZCs) (59.6%). In terms of discomfort symptoms, 105 (76.7%) and 102 (76.1%) cases were reported after PMC and ZC placement respectively; however, there was no statistically significant difference between the two groups (P > 0.05). Among the surveyed parents, 19 (8.7%) expressed that they could not accept the PMC color, whereas all could accept the ZC color, and the difference was statistically significant (P < 0.05). A total of 217 parents (99.5%) could accept PMCs to protect or repair primary carious teeth; 29.8% of the parents stated that they were reluctant to use ZCs to protect or repair their children's deciduous teeth, and the difference was significant (P < 0.05). In terms of location selection, the majority of surveyed parents chose comprehensive hospitals (58.7%) and stomatological hospitals (41.3%). In terms of institution selection, priority was given to the doctor's qualifications (90.4%). Pearson correlation analysis revealed that "acceptance of the PMC color", "oral health awareness", and "parental willingness to use PMCs" were positively correlated (P < 0.05) and that "non-nuclear family status" was negatively related to "parental willingness to use PMCs" (P < 0.05). The logistic multiple regression analysis revealed that oral health awareness and restoration awareness significantly affected the duration of discomfort symptoms after PMC placement (P < 0.15). Children with higher annual family incomes, families with fewer children, lower consumption of desserts, better oral health awareness, and teeth brushing under the assistance of their parents showed a dramatic decline in untimely PMC repair (P < 0.15); children who brushed their teeth less and whose parents had a poorer perception of restoration methods showed an increase in untimely PMC repair (P < 0.15). Conclusions: This discrepancy is reflected in the cognition and willingness to use PMCs and ZCs. PMCs are more accepted due to cost, whereas ZCs are preferred for aesthetics. The factors affecting PMC restoration are oral health awareness, manner of restoration, family income, number of children, consumption of desserts, and toothbrushing frequency. [ABSTRACT FROM AUTHOR]

Introduction: The association between breastfeeding status and early childhood caries (ECC) remains inconclusive. Few studies evaluate the breastfeeding status including both duration and exclusivity according to the WHO recommendations on breastfeeding. This study aimed to investigate the association between breastfeeding status and ECC. Methods: This prospective cohort study comprised 3,666 children whose breastfeeding status was precisely evaluated. Poisson regression models and multivariable linear regression models were employed to analyze the associations of breastfeeding status with risk of ECC, and mean decayed, missing, and filled primary tooth surfaces (dmfs) in 3-year-old children, respectively. The data were collected from 2014 to 2020, and the analysis was conducted in 2023. Results: The prevalences of ECC in offspring breastfed for <6 months, 6–11 months, 12–24 months, and >24 months were 9%, 12%, 17%, and 23%, respectively. Offspring breastfed for 12–24 months and over 24 months had a 1.82 times (95% CI, 1.40–2.37; p < 0.001) and 2.48 times (95% CI, 1.63–3.75; p < 0.001) higher risk of ECC, compared to those breastfed for less than 6 months. Offspring breastfed for 12–24 months showed a mean dmfs increase of 0.32 (95% CI, 0.21–0.44; p < 0.001), while those breastfed for over 24 months had a mean dmfs increase of 0.51 (95% CI, 0.27–0.74; p < 0.001), compared to those breastfed for less than 6 months. Among offspring breastfed for over 24 months, those exclusively breastfed for at least 6 months had significantly lower mean dmfs compared to those exclusively breastfed for less than 6 months (p for heterogeneity = 0.003). A significant interaction was observed between breastfeeding duration and exclusive breastfeeding duration on the association with mean dmfs (p for interaction <0.001). Conclusion: Our findings suggest that breastfeeding for over 12 months was associated with increased risk of ECC. Preventive interventions for dental caries should be implemented as early as possible, as breastfeeding is beneficial to children’s health. The associations between breastfeeding duration and exclusivity with ECC should be investigated more thoroughly, particularly with adjustments for accurately measured sugar consumption. [ABSTRACT FROM AUTHOR]

Background: The study exploited, for the first time, Attenuated Total Reflectance-Fourier Transform-InfraRed (ATR-FTIR) spectroscopy on human dental pulps at different timings of root resorption (RR) to deepen the biological mechanisms occurring in deciduous teeth (De) during their replacement with permanent ones. Methods: N:36 dental pulps from sound De were divided into the following: G0 (no RR); G1 (RR less than 1/3 of root length); G2 (RR not exceeding 2/3 of root length); and G3 (RR more than 2/3 of root length). Samples were analyzed by ATR-FTIR, and the spectral data were submitted to univariate (One-way ANOVA and Tukey's multiple comparison tests; statistical significance set at p < 0.05) and multivariate (Principal Component Analysis, PCA) analyses. Results: PCA displayed good discrimination among groups, ascribable to: (i) the intensity of the peaks of nucleic acids (~1715 cm−1, ~1237 cm−1, ~964 cm−1, and ~815 cm−1) and carbohydrates (~1159 cm−1) which increased from G0 to G3 (p < 0.05); (ii) the relative amount of lipids which decreased from G0 to G3 (p < 0.05); and (iii) the intensity of the peaks at ~1014 cm−1, and ~875 cm−1 (phosphates and carbonates in hydroxyapatite), which decreased from G0 to G3 (p < 0.05). Conclusions: This study confirmed ATR-FTIR as a reliable and quick technique for the characterization of the dental pulp and highlighted a correlation between specific molecular changes in the dental pulp of deciduous teeth and different RR stages, shedding new light on this process and paving the way for future research, which could improve the clinical management of the primary dentition. [ABSTRACT FROM AUTHOR]

ABSTRACT Background/Aim Materials and Methods Results Conclusions Avulsion injuries have an uncertain prognosis. This study aims to evaluate periodontal ligament healing patterns and factors affecting the survival of replanted avulsed teeth.In this retrospective study, data from 204 avulsed teeth in 143 children (mean age: 9.6 ± 2 years) who sustained avulsion injuries were reviewed. Primary teeth, non‐replanted permanent teeth, and cases with follow‐up shorter than 60 days were excluded. Data analysis included 60 replanted permanent teeth from 41 patients (18 girls and 23 boys), with a follow‐up period ranging from 2 to 147 months. Collected data covered age, gender, extra‐alveolar storage time, root development stage, and periodontal ligament healing patterns. Statistical analysis was performed on the data.Avulsion injuries predominantly occurred during outdoor activities (n = 22, 59.5%), followed by school (n = 10, 27%) and home (n = 5, 13.5%). The main causes were falls (n = 20, 50%), bicycle accidents (n = 6, 15%), sports injuries (n = 5, 12.5%), traffic accidents (n = 2, 5%), and violence (n = 1, 2.5%). Among replanted teeth, 51.7% were immature. Treatments included root canal therapy (n = 30, 50%), apexification (n = 12, 20%), and regeneration (n = 2, 3.3%), while 16 teeth received no treatment. Inflammatory resorption was not observed in teeth replanted within 1 h of injury (8.4%). Functional healing/ankylosis was observed in 14 (23.3%) of the replanted teeth, replacement resorption in 40 (66.7%), and inflammatory resorption in 6 (10%). Replacement resorption affected 66.7% of the replanted teeth, with 62.5% lost over time. Functional healing/ankylosis was more common in mature teeth, while inflammatory resorption was higher in immature teeth. According to Kaplan–Meier estimated survival curves, the survival rate during the 5‐year observation period was 47.5%. Survival times were longer in boys and in mature teeth (p < 0.05).Gender and root development stages were observed to significantly impact the prognosis of replanted avulsed teeth. [ABSTRACT FROM AUTHOR]

Engineering spheroids to create three-dimensional (3D) cell cultures has gained increasing attention in recent years due to their potential advantages over traditional two-dimensional (2D) tissue culture methods. Stem cells derived from human exfoliated deciduous teeth (SHEDs) demonstrate significant potential for pulpal regeneration applications. Nevertheless, the feasibility of microsphere formation of SHEDs and its impact on pulpal regeneration remain unclear. In this study, SHEDs were isolated, identified, and cultured in ultra-low attachment six-well plates to produce SHED microspheres. The biological properties of SHED microspheres were compared to those of traditional 2D culture using live-dead staining, Alizarin red staining, Oil-red O staining, scratch experiments, Immunofluorescence, Transmission electron microscopy scan, Western blotting, RNA sequencing, and a nude mice subcutaneous transplantation model. We found SHED cells can form microspheres with a dense internal structure. SHED microspheres exhibited notable advantages over SHED cells in terms of biological properties, maintaining cell activity and enhancing cell differentiation, migration, and stemness in vitro. RNA-seq revealed that the SHED microspheres potentially influenced cell development, regulation of neurogenesis, skeletal system development, tissue morphogenesis singling pathway. In vivo, SHED microspheres promoted the generation of pulp tissue in dental pulp compared to traditional 2D culture. Microsphereization of SHED through 3D cell culture enhances its pulp regeneration capacity, presenting a novel strategy for dental pulp regeneration and the clinical treatment of dental pulp diseases. • SHED microspheres could improve the stemness, differentiation, and migration of SHED in vitro. • SHED microspheres could enhance its pulp regenerative effect in vivo. • SHED microspheres presenting a novel strategy for dental pulp regeneration and the clinical treatment of dental pulp diseases. [ABSTRACT FROM AUTHOR]

The recent World Health Organization (WHO) Oral Health Resolution and the subsequent WHO Global Oral Health Action Plan highlight the key relevance of providing information on the economic impacts of oral conditions. The purpose of this study was to provide updated estimates for the global, regional, and country-level economic impacts of oral conditions in 2019. Extending previously established methods, dental expenditures (costs for treatments) and productivity losses for 5 oral conditions (caries in deciduous and permanent teeth, periodontitis, edentulism, other oral diseases) were estimated for the year 2019. The estimated total worldwide economic impacts of oral conditions in 2019 were US $710B, of which US $387B (US $327B to US $404B) was due to direct costs and US $323B (US $186 to US $460) was due to productivity losses for the 5 main oral conditions. Low-income countries spent an average of US $0.52 (US $0.22 to US $0.96) per capita on dental care, while high-income countries spent an average of US $260 (US $257 to US $268) per capita—a 500-fold difference. These findings suggest that oral conditions continue to substantiate an enormous economic burden to individuals and society. The comprehensiveness of estimates supersedes that of previous work as the primary information on direct costs was identified for a larger number of countries. The need for more and better routine reporting and monitoring of the economic impact of oral conditions is emphasized. The relevance of such information is also highlighted by its inclusion in the first-ever WHO Global Oral Health Status Report and Global Strategy on Oral health 2023 to 2030. Given the persistently high economic burden of oral conditions, there is a key role for better prioritization of cost-efficient oral health programs as well as needs-responsive capacity planning. [ABSTRACT FROM AUTHOR]

Dental trauma is common in all age groups, although, epidemiologically, it is more common in children with studies that indicate that 15% of preschoolers and 20–25% of school-age children experience it. These injuries, which frequently call for immediate attention, can affect the hard tissues and supporting components of the teeth, and, because dental damage in deciduous teeth occurs frequently and affects speech, nutrition, and oral development, it is particularly worrying. After searching three databases, Scopus, Web of Science (WoS), and PubMed, and removing duplicates, 3,630 articles were screened, and 12 publications were included in the qualitative analysis. Due to their busy lifestyles, children are particularly susceptible to oral trauma and in certain areas and lower socioeconomic groups, the incidence is higher. From little fractures in the enamel to serious dislocations and avulsions, injuries vary and must be treated promptly in order to avoid consequences and to prevent long-term issues. Furthermore, a conservative treatment strategy is recommended to preserve tooth vitality and prevent extractions. Reducing the occurrence of dental injuries requires the implementation of preventive measures including mouthguard use and educational campaigns. In summary, this review emphasizes the importance of early diagnosis, immediate management, and long-term care, by synthesizing existing knowledge on the prevalence, types, management, complications, and prevention of dental trauma in deciduous teeth. Finally, it's important to underscore the need for continued research to refine treatment approaches. [ABSTRACT FROM AUTHOR]

Hair graying is one of the common visible signs of human aging, resulting from decreased or abolished melanogenesis due to the depletion of melanocyte stem cells through excess accumulation of oxidative stress. Cell-free therapy using a conditioned medium (CM) of mesenchymal stem cells has been highlighted in the field of regenerative medicine owing to its potent therapeutic effects with lower regulatory hurdles and safety risk. Recently, we demonstrated that a CM of an immortalized stem cell line from human exfoliated deciduous teeth (SHED) has protective effects against a mouse model of ulcer formation via antioxidative and angiogenic activities mediated by HGF and VEGF. However, to date, no effective treatments for hair graying have been developed, and the effect of SHED-CM on hair graying remains unknown. In this study, we have investigated the effect of SHED-CM on a hair graying mouse model caused by X-ray irradiation. Repetitive subcutaneous administrations of SHED-CM greatly suppressed the development of hair graying, when compared to control medium, resulting in reduced cutaneous expression of 8-hydroxy-2′-deoxyguanosine, the major product of DNA damage induced by reactive oxygen species. Consistent with these in vivo results, SHED-CM significantly inhibited the cell death caused by X-ray irradiation in melanoma cell line B16F10 cells. Immunodepletion of HGF or VEGF in the SHED-CM revealed that this inhibition was due to suppression of the generation of reactive oxygen species, which was mainly mediated by HGF and probably VEGF. These results suggest that SHED-CM has protective effects against hair graying via its antioxidative activity. [ABSTRACT FROM AUTHOR]

Background: Additively and subtractively manufactured resin-based crowns have emerged as a new option for pediatric patients. In this study, it was aimed to test the effect of the production technique on the marginal and internal gap values of resin-based crowns for primary teeth. Methods: A total of 30 crowns were produced with additive (3D printing; DLP and SLA) and subtractive (milling) technologies (n = 10). The crowns were cemented on the resin dies. Three STL files were obtained from the scans of the die, crown, and crown cemented on the die. STL data of the die and crown were superimposed on the STL data of the crown cemented on the die, and gap volume calculations in the marginal and internal areas were performed in the software. The results were statistically analyzed with one-way ANOVA and Tukey HSD tests (α = 0.05). Results: The results showed that the production technique was significantly effective on the marginal and internal gap values. The highest marginal and internal gap value was observed in the milling group, followed by the SLA and DLP groups. Conclusion: The production technique affected the fit of the resin-based crowns for primary teeth. Compared to subtractive techniques, lower marginal and internal gap volumes were found with additive production techniques. [ABSTRACT FROM AUTHOR]

Background: Evaluating the effectiveness and survival rates of space maintainers is crucial for optimal pediatric dental care. The present study's goal is to evaluate the survival rate of different types of space maintainers—removable, fixed with a metal base, or fixed with a resin base—indicated for children as a consequence of premature loss of primary teeth. Methods: A systematic review was conducted in accordance with PRISMA recommendations and was previously registered in PROSPERO under registration number: CRD42024534183. The search was performed in five different databases PubMed, EMBASE, Scopus, Cochrane, and Web of Science. This was supplemented by a manual database search. From the initial electronic search of the five databases, 72 articles were identified after removing duplicates. After reading the titles and abstracts, 46 articles were excluded, leaving a total of 26 studies. Upon reading the full texts, 15 were excluded for not meeting the inclusion criteria, resulting in a total of 11 articles included in the systematic review. Results: The total number of patients treated in the studies was 479, with four observational studies and seven randomized clinical trials. Fixed space maintainers, such as band and loop (BL) or lingual arch (LA), are preferred due to their long-term stability and lower risk of loss. In contrast, removable maintainers, such as Hawley plates, are suitable temporarily but require more patient cooperation and may be less tolerated. Conclusions: Space maintainers help prevent the migration of adjacent teeth and the need for more invasive orthodontic treatments in the future. Additionally, they contribute to adequate masticatory function and speech development. [ABSTRACT FROM AUTHOR]

Objective This study aimed to investigate the effect of hydroxyapatite from snakehead (Channa striata) fish bone on the surface hardness of the enamel of the primary teeth. Materials and Methods Twenty-six primary maxillary incisors were mounted on self-cured acrylic resin, divided into two groups, and demineralized. Remineralization was performed using hydroxyapatite paste synthesized from C. striata fish bone by the precipitation method. The case group was subjected to 15% hydroxyapatite paste and subsequently submerged in artificial saliva, while the control group was only exposed to artificial saliva. Enamel hardness was measured by the Vickers hardness tester after 7 days of treatment. The statistical analysis used in this research was an independent t-test. Results The case group had a surface hardness of 356.192 ± 25.218, and the control group had a surface hardness of 269.686 ± 22.931. Statistical tests showed a significant difference between the case and control groups. Conclusion Hydroxyapatite paste from snakehead (C. striata) fishbone stimulates remineralization of primary teeth, as evidenced by an increase in the enamel surface hardness of the teeth. [ABSTRACT FROM AUTHOR]

Background: Primary teeth segmentation on cone beam computed tomography (CBCT) scans is essential for paediatric treatment planning. Conventional methods, however, are time‐consuming and necessitate advanced expertise. Aim: The aim of this study was to validate an artificial intelligence (AI) cloud‐based platform for automated segmentation (AS) of primary teeth on CBCT. Its accuracy, time efficiency, and consistency were compared with manual segmentation (MS). Design: A dataset comprising 402 primary teeth (37 CBCT scans) was retrospectively retrieved from two CBCT devices. Primary teeth were manually segmented using a cloud‐based platform representing the ground truth, whereas AS was performed on the same platform. To assess the AI tool's performance, voxel‐ and surface‐based metrics were employed to compare MS and AS methods. Additionally, segmentation time was recorded for each method, and intra‐class correlation coefficient (ICC) assessed consistency between them. Results: AS revealed high performance in segmenting primary teeth with high accuracy (98 ± 1%) and dice similarity coefficient (DSC; 95 ± 2%). Moreover, it was 35 times faster than the manual approach with an average time of 24 s. Both MS and AS demonstrated excellent consistency (ICC = 0.99 and 1, respectively). Conclusion: The platform demonstrated expert‐level accuracy, and time‐efficient and consistent segmentation of primary teeth on CBCT scans, serving treatment planning in children. [ABSTRACT FROM AUTHOR]

Mesenchymal stem cells (MSCs) have been widely used in the treatment of various inflammatory diseases. The inadequate understanding of MSCs and their heterogeneity can impact the immune environment, which may be the cause of the good outcomes of MSCs-based therapy that cannot always be achieved. Recently, stem cells from human exfoliated deciduous teeth (SHED) showed great potential in inflammatory and autoimmune diseases due to their immature properties compared with MSCs. In our study, single-cell RNA sequencing (scRNA-seq) revealed that SHED in a low differentiation state (S7) exhibited the powerful ability to recruit multiple immune cells. In contrast, SHED in a relatively high differentiation state (S1) may hold a solid ability to secret many factors with paracrine signaling capacity. The analysis result shows that SHED has more robust immunomodulatory properties than human bone marrow-derived mesenchymal stem cells (hBMSCs) or human umbilical cord-derived mesenchymal stem cells (hUCMSCs). When co-cultured with PBMCs, SHED can enhance the proliferation of Treg and down-regulate TNF-α in vitro. SHED may have some advantages in the treatment of inflammatory and autoimmune diseases. [ABSTRACT FROM AUTHOR]

ABSTRACT This article presents and discusses isotopic data on breastfeeding, weaning, and complementary diet during early childhood in Middle Holocene southeastern Brazil. The study focuses on human individuals from two contemporaneous shell mounds, Piaçaguera (7151–5668 yBP) and Moraes (6791–5590 yBP), which show distinct patterns of infant mortality. We conducted δ15N and δ13C analyses on collagen extracted from subadult bones (Piaçaguera: n = 13; Moraes: n = 13) and sequential dentine slices from permanent (Piaçaguera: n = 7; Moraes: n = 11) and deciduous teeth (Moraes: n = 5). Our findings indicate that weaning ended between 2 and 4 years old in Piaçaguera, with complementary foods similar to the adults. At Moraes, weaning ended between 2 and 5 years old, with a higher consumption of freshwater fish during early childhood and by pregnant women. The weaning ages at Piaçaguera and Moraes align with those documented in other precolonial South American groups. However, our study suggests different dietary preferences and strategies within each group, possibly linked to distinct patterns of infant mortality, particularly evident in the Moraes group. [ABSTRACT FROM AUTHOR]

Mild hypophosphatasia (HPP) can be difficult to distinguish from other bone disorders in the absence of typical symptoms such as the premature loss of primary teeth. Therefore, this study aimed to analyze the crystallinity of hydroxyapatite (HAp) and the three-dimensional structure of collagen in HPP teeth at the molecular level and to search for new biomarkers of HPP. Raman spectroscopy was used to investigate the molecular structure, composition, and mechanical properties of primary teeth from healthy individuals and patients with HPP. The results showed that the crystallinity of HAp decreased and the carbonate apatite content increased in the region near the dentin–enamel junction (DEJ) of HPP primary teeth. X-ray diffraction (XRD) analyses confirmed a decrease in HAp crystallinity near the DEJ, and micro-computed tomography (CT) scanning revealed a decrease in mineral density in this region. These results suggest incomplete calcification in HPP primary dentin and may contribute to the development of diagnostic and therapeutic agents. [ABSTRACT FROM AUTHOR]

\nIntroduction: The aim of this in vitro study was to evaluate the accuracy of near-infrared imaging (NIRI) generated by the iTero Element 5D scanner for detecting proximal caries lesions in deciduous molars, compared with visual inspection (VI), bitewing (BW) radiography, and histological examination (reference standard). Methods: Sound deciduous molars and those with caries lesions (ICDAS 0 to 5) on the proximal surface were included, while teeth with marginal crest breakdown, restoration on the mentioned surfaces, or extensive dentin resorption were excluded. A total of 182 deciduous molars, divided into 91 pairs of first and second molars, were evaluated by two blinded examiners. Two distinct thresholds were considered for the assessment of caries detection methods: enamel lesion (D1) and dentin lesion (D3). Specificity, sensitivity, accuracy, and area under the ROC curve (AUC) were calculated for each method in the statistical analysis. Results: All evaluated methods demonstrated high specificity at D1, with 1.00 for VI and 0.95 for NIRI and BW, and at D3, 0.99 for BW and 0.98 for NIRI and VI, without statistical differences. The sensitivity and accuracy of NIRI at D1 were 0.44 and 0.55, while those of VI were 0.46 and 0.58, and of BW were 0.60 and 0.68, respectively. And sensitivity and accuracy of NIRI at D3 were 0.14 and 0.69, while those of VI were 0.37 and 0.77, and of BW were 0.51 and 0.82, respectively. No difference in sensitivity and accuracy was observed between NIRI and VI at D1 (p = 0.589); however, NIRI presented the lowest accuracy at D3. At D1, no statistical difference was observed between the AUC of BW and VI (p = 0.1124), nor between NIRI and VI (p = 0.2523). However, at D3, statistical differences were observed between the AUCs of the three evaluated methods: VI versus NIRI (p = 0.0005), VI versus BW (p = 0.0281), and NIRI versus BW (p < 0.0001). Conclusion: NIRI showed accuracy comparable to VI at the D1 threshold but was less effective than BW radiography at both D1 and D3 thresholds. Tooth decay is the most prevalent chronic disease in childhood, and early detection of proximal lesions is essential for implementing a treatment plan based on minimal intervention. The aim of this in vitro study was to evaluate the accuracy of the nonionizing method near-infrared imaging (NIRI) generated by the iTero Element 5D scanner for detecting proximal caries lesions in deciduous molars, comparing it with visual inspection and bitewing radiography. The gold standard was histological analysis. A total of 182 deciduous molars, divided into 91 pairs of first and second molars, were evaluated by two blinded examiners. Teeth with sound proximal surfaces and carious lesions (ICDAS 0–5) were included, while teeth with restorations, marginal crest breakdown on the mentioned surfaces, and extensive dentin resorption were excluded. Two distinct thresholds were considered for the assessment of caries detection methods: enamel lesion (D1) and dentin lesion (D3). Sensitivity (ability of a method to correctly identify carious lesions when they are present), specificity (ability of a test to correctly identify healthy surfaces without carious lesions), accuracy (combines sensitivity and specificity, representing the overall ability of a method to provide correct diagnoses), and area under the ROC curve (illustrates how sensitivity and specificity vary) were calculated for each method in the statistical analysis. We conclude that NIRI was able to detect caries lesions similarly to visual inspection for lesions located in dental enamel but was less effective than bitewing radiography for lesions in both enamel and dentin. [ABSTRACT FROM AUTHOR]

Background: XP‐endo files are composed of Max‐Wire alloy, which was developed by FKG Dentaire (La Chaux‐de‐Fonds, Switzerland). This alloy, known as Martensite‐Austenite Electropolish Flex, is the first NiTi alloy used in endodontics to combine the shape memory effect with superelasticity for use in clinical practice. Objective: This article aims to provide a comprehensive overview of the existing knowledge and evidence regarding different XP‐endo files and systems, XP‐endo Finisher (XPEF), XP‐endo Shaper (XPES), XP‐endo Retreatment (XPER), XP‐endo Rise (XPE‐Rise), and XPE‐Rise Retreatment (XPE‐Rise‐R), to help clinicians understand their different properties and recommended clinical uses. Moreover, this review aims to identify future research opportunities in this field. Materials and Methods: A search was performed in the PubMed database using the keywords "XP endo" or "XP‐endo". A total of 309 studies were identified during the initial search, and then initial abstract screening was conducted. The inclusion criteria included any study that aimed to evaluate XP endo files and systems on permanent teeth, either in vivo or in vitro. We excluded studies that were unrelated to the subject, literature reviews, case studies, and studies that employed deciduous teeth. Finally, we performed an extensive review of the selected 130 studies, which we assessed, summarized, and classified based on the specific XP‐endo file used. Conclusion: Overall, XP‐endo files offer significant improvements in endodontic treatment. XPEF excels in irrigation activation and medicament removal, and XPES succeeds in canal cleaning, shaping, and retreatment. The XPER system, which incorporates the XPEF‐R file, shows promise in removing root‐filling materials, but its performance is inconsistent compared to other retreatment systems. We have found limited information regarding the latest XPE‐Rise and XPE‐Rise‐R systems, additional research is required to fully determine their comparative effectiveness and optimize their clinical application. [ABSTRACT FROM AUTHOR]

ABSTRACT When the Dutch ship Batavia wrecked in 1629 on Morning Reef in the Houtman Abrolhos Islands off the coast of Western Australia, most of the passengers and crew survived the wreck, but about 200 of the survivors later perished on and around Beacon Island where they had landed after the wreck. As many as 81 bodies may be buried on Beacon Island and 21 bodies have been excavated to date. During the 2017–2018 excavations, seven of the buried individuals were excavated from a communal grave. During the excavation, 18 teeth were found scattered in the soil around the bodies. A systematic method is presented to allocate isolated teeth to their appropriate skeleton using tooth morphology and tooth wear as the primary criteria for allocation. Tooth wear must be similar to the adjacent and opposing dentition before the tooth is considered for allocation. If the wear is similar, the tooth is gently fitted into the proposed tooth socket. The fit of the tooth in the tooth socket is visually assessed, and if close, the tooth is radiographed in situ to confirm the allocation. Each separated tooth must be tested in all the sites to which it is appropriate. If a tooth fits more than one site, it cannot be allocated. Use of this method allowed allocation of 10 separated teeth in 28 possible sites. The possible cause of the separation of teeth from the skeleton is discussed. [ABSTRACT FROM AUTHOR]

ABSTRACT Background/Aim Materials and Methods Results Conclusions The aim of this study was to investigate the impact of traumatic dental injury (TDI) on oral health‐related quality of life (OHRQoL) of preschool children, and further inform public oral health care policies and strategies for this age group TDI patients.This retrospective and cross‐sectional study was conducted in the Department of Pediatric Dentistry, West China Hospital of Stomatology, Sichuan University, Chengdu, China. It included 314 children aged from 2 to 5 years, with 90 children diagnosed with TDIs and 224 without TDIs. Parents or primary caregivers completed questionnaires, and the responses were gathered for statistical analysis to evaluate the impact of TDIs. The OHRQoL was assessed using the Early Childhood Oral Health Impact Scale (ECOHIS).The total ECOHIS score and the scores for the 13 individual items were significantly higher in children with TDIs compared with those without TDI (p < 0.001). In the child impact section, eating and masticatory function were the most severely affected (40%), while most of parents experienced strong feelings of self‐blame in the family impact section (61.1%). A positive correlation was observed between the number of injured teeth and the total ECOHIS score (p = 0.001), while the time elapsed since TDI indicates a negative correlation with the total ECOHIS score (p < 0.001). Additionally, children with severe TDIs had higher ECOHIS scores than those with mild TDIs, indicating a greater impact on OHRQoL (p = 0.006). In contrast, tooth discoloration, the caregiver's relationship to the child, caregiver education level and the position of the affected teeth did not significantly influence the total ECOHIS score (p > 0.05).TDIs have a significant impact on OHRQoL in preschool children, highlighting the need for greater societal attention. Strengthening preventive and proper treatment measures for TDIs, along with enhancing health education programs, is strongly recommended. [ABSTRACT FROM AUTHOR]

Background: A Partial pulpotomy technique is an alternative choice of treatment for immature permanent teeth with exposed vital pulps. This study aimed to compare the histopathological pulpal response of the primary teeth of puppies after partial pulpotomy and full pulpotomy using mineral trioxide aggregate (MTA). Materials and methods: 72 primary premolars from experimental puppies aged 6–8 weeks were equally divided into test and control groups, as MTA partial pulpotomy (MTA-PP) and MTA full pulpotomy (MTA-FP). After 60 days, the teeth were extracted and examined histologically. The specimens were assessing the extent and intensity of inflammatory response (acute or chronic), necrosis, fibrosis, calcific bridge (presence or absence), pulp calcification, and pathological root resorption. Results: The Inflammation intensity was lower (2.8%) in MTA-PP than in MTA-FP (16.7%) (P = 0.004). MTA-FP also had a significantly higher percentage of localized (27.8%) and diffuse (11.1%) necrosis (P = 0.014), whereas both groups (P = 0.290) had an equal fibrosis degree. A calcific bridge was observed in 72.2% of cases. Meanwhile, pathological internal root resorption was more evident in MTA-FP (63.9%) than in MTA-PP (19.4%) (P <0.0001). Conclusion: Partial MTA pulpotomy procedure demonstrated promising histological findings that can be applied to vital-pulp therapy in primary teeth. [ABSTRACT FROM AUTHOR]

Background: Pulpally infected primary teeth should be retained until exfoliation whenever possible, as deciduous teeth are ideal space maintainers. Removing bacteria from the root canal is essential for a successful root canal treatment. Therefore, the present study compared the antimicrobial effects of resorbable bioceramic sealer with pastes of calcium hydroxide and iodoform mixed with zinc oxide and zinc oxide eugenol when used as intracanal medication on microorganisms of necrotic primary molars. The materials used in the study were: Paste A: Non-setting calcium hydroxide paste mixed with iodoform, Paste B: Zinc oxide eugenol, Paste C: Metapex with zinc oxide, Paste D: Metapex with zinc oxide eugenol, Paste E: Bio-C Pulpecto. Polymicrobial growth inhibition zone diameters around each of the five pastes were measured in mm to determine antimicrobial activity. The collected data were presented, tabulated, and statistically analyzed. Results: Comparison among all groups demonstrated a significant difference between them (P < 0.0001) using the Kruskal–Wallis test. Furthermore, Dunn's multiple comparisons test revealed that: Paste C (0.93 ± 3.55) and Paste A (2.13 ± 2.67) exhibited significantly the lowest antibacterial activity with no significant difference between them. Subsequently, Paste D (5.2 ± 5.89) and Paste E (8.73 ± 1.36) displayed no significant difference between them. In contrast, Paste B (11.8 ± 1.58) demonstrated significantly the highest antibacterial activity. Conclusions: Bioceramic sealers could be utilized as filling material for root canal in primary molars because of their antimicrobial and biological properties. [ABSTRACT FROM AUTHOR]

The objective of this study was to identify, describe and synthesize the data published about the effectiveness of antimicrobial photodynamic therapy (aPDT) in the pulpectomy of primary teeth. A search was conducted in six electronic databases and the gray literature in June and July 2023, without restrictions on language or date of publication. The search strategy included indexed terms (MeSH terms) and your synonyms. Clinical trials, in vitro studies, case reports and retrospective studies that used photodynamic therapy in primary teeth pulpectomies were considered eligible. Among the 1,674 articles retrieved, 15 were included, published between 2014 and 2022, comprising six in vitro studies, five clinical trials, and four case reports. The sample consisted of children aged 2 to 8 years of both sexes, and included 1 to 62 teeth, mostly anterior. In two in vitro studies, the sample was taken from root canals. Most studies used a diode laser, methylene blue, and optical fiber. The pre-irradiation time ranged from 1 to 5 min. All protocols demonstrated microbial reduction before and after using aPDT, with no statistical difference among the groups. Furthermore, six studies demonstrated treatment success. Despite the divergence in methodology and protocols, aPDT is an effective method for reducing microorganisms, and is a promising adjunct treatment in primary teeth pulpectomies. [ABSTRACT FROM AUTHOR]

Background: Helicobacter pylori is a persistent pathogen in the human stomach. However, the proposed transmission route via the oral cavity is not understood and under intense debate. While dozens of studies have shown by PCR that H. pylori DNA is frequently present in the oral cavity, data on the growth and characterization of viable H. pylori from this compartment are very scarce, and it is unclear whether the bacteria can survive in the oral cavity for longer time periods or even colonize it. Methods: Selective growth methods, scanning electron microscopy, urease assay, Western blotting, PCR, and gene sequencing were applied to identify and examine viable H. pylori in decayed milk teeth. Results: Here, we studied viable H. pylori in the plaque and root canals of 170 endodontically infected deciduous teeth that were extracted from 54 children. While H. pylori DNA was detected in several plaque and many root canal samples by PCR, live bacteria could only be cultivated from 28 root canals, but not from plaque. These 28 isolates have been identified as H. pylori by PCR and sequencing of vacA, cagA and htrA genes, phylogenetic analyses, protein expression of major H. pylori virulence factors, and by signal transduction events during infection of human cell lines. Conclusions: Thus, the microaerobic environment in the root canals of endodontically infected teeth may represent a protected and transient reservoir for live H. pylori, especially in individuals with poor dental hygiene, which could serve as a potential source for re-infection of the stomach after antibiotic therapy or for transmission to other individuals. [ABSTRACT FROM AUTHOR]

Spacing between teeth is a common trait across different stages of dentition. With the tide of the digital impression, the scanning trueness of the intraoral scanner (IOS) is a hot subject. This study aimed to determine the correlation between the level of the spaced dentition and trueness of the intraoral scanning. Four arch lengths of deciduous tooth models (spacing Model 1, Model 2, Model 3, Model 4 = 0, 1, 2, 3 mm; maxillary arch length = 73.268, 81.922, 90.776, 97.698 mm; mandibular arch length = 69.092, 76.160, 86.228, 94.344 mm) were designed to measure trueness via an IOS. Statistical analysis included one-way analysis of variance followed by post hoc Tukey tests for comparisons of the data. The trueness varied across different levels of spacing, with the highest deviation observed between intraoral and desktop scans in Model 3, followed by Model 4, Model 2, and Model 1 in the maxillary arch. In the mandibular arch, the sequence of deviation from highest to lowest was Model 4, Model 3, Model 1, and Model 2. Significant differences were observed among these models in both the maxilla and mandible (P < 0.001). In both the maxilla (2 mm spacing, arch length ≥81.922 mm) and mandible (3 mm spacing, arch length ≥86.228 mm), scanning accuracy decreases with longer arch lengths. This indicates that as arch length increases, so does the deviation in scanning accuracy. Therefore, the clinician should notice the deviation when using IOSs for the spaced cases. [ABSTRACT FROM AUTHOR]

Background: Dental pulp (DP) is a connective tissue composed of various cell types, including fibroblasts, neurons, adipocytes, endothelial cells, and odontoblasts. It contains a rich supply of pluripotent stem cells, making it an important resource for cell-based regenerative medicine. However, current stem cell collection methods rely heavily on the enzymatic digestion of dissected DP tissue to isolate and propagate primary cells, which often results in low recovery rates and reduced cell survival, particularly from deciduous teeth. Methods: We developed a novel and efficient method to obtain a sufficient number of cells through a one-step cultivation process of isolated DP. After the brief digestion of DP with proteolytic enzymes, it was scratched onto a culture dish and cultured in a suitable medium. By day 2, the cells began to spread radially from DP, and by day 10, they reached a semi-confluent state. Cells harvested through trypsinization consistently yielded over 1 million cells, and after re-cultivation, the cells could be propagated for more than ten passages. Results: The proliferative and differentiation capacities of the cells after the 10th passage were comparable to those from the first passage. The cells expressed alkaline phosphatase as an undifferentiation marker. Similarly, they also maintained the constitutive expression of stem cell-specific markers and differentiation-related markers, even after the 10th passage. Conclusions: This method, termed "scratch-based isolation of primary cells from human dental pulps (SCIP)", enables the efficient isolation of a large number of DP cells with minimal equipment and operator variability, while preserving cell integrity. Its simplicity, high success rate, and adaptability for patients with genetic diseases make it a valuable tool for regenerative medicine research and clinical applications. [ABSTRACT FROM AUTHOR]

Background: Oral health outcomes in children with Congenital Heart Defects (CHD) have significant implications. The aim of this systematic review and meta-analysis is to update the current understanding of oral health outcomes in children with CHD and compare caries prevalence between CHD children and healthy controls. Methods: All studies between 2014 and 2024 comparing oral health status between children with and without CHD were considered for inclusion. Studies had to use the DMF/dmf index (Decayed, Missing, Filled Teeth or Surface index), in permanent and deciduous teeth. Two separate meta-analyses were conducted: one analyzing DMFS scores and another focusing on dmft scores. Medline, Central, and Embase databases were screened. Twelve articles were included in the qualitative synthesis, and two studies were finally included in each quantitative synthesis. Results: Several studies identified significant differences in oral health outcomes, suggesting that children with CHD are at a higher risk of dental caries compared with healthy controls, particularly as they become older. However, the differences were not uniformly observed across all studies and age groups. Based on the meta-analysis, there was no statistically significant difference in either DMFS scores (MD: 0.07 [95% CI: −0.13, 0.27]; p = 0.48) or in dmft scores (MD: 1.39 [95% CI: −1.05, 3.83]; p = 0.26). Conclusions: This systematic review and meta-analysis highlight a possible increased risk of dental caries in children with CHD, although results were not statistically significant and varied across studies. More standardized and rigorous studies are required to provide clearer insights into oral health outcomes for this population. [ABSTRACT FROM AUTHOR]

Background: Dental caries is one of the most prevalent dental illnesses in children. Untreated severe caries can damage teeth, requiring pulpotomy to save them. Objective: To evaluate the clinical success rate of treatments involving pulpotomies + IRM (Intermediate Restorative Material, composed of reinforced zinc oxide–eugenol polymers) + preformed metal crowns (PMCs) in primary teeth of children treated at a public university in Mexico. Materials and Methods: A cross-sectional ambispective study was conducted on children aged 10 years or younger, treated at a public university in Mexico. A total of 1281 medical records from February 2018 to June 2023 were reviewed, yielding a sample of 199 children treated with pulpotomy + IRM + PMC. Information was collected via telephone interviews with parents/caregivers to inquire about any symptoms following the pulpotomy and metal crown treatments. Success was defined as the absence of pain, inflammation, or infection. Results: During the study period, the number of teeth treated with pulpotomy + IRM + PMC was 414, with follow-ups ranging from 6 to 69 months. Most participants were girls (54.3%), while 45.7% were boys. Out of the 167 pulpotomy + IRM + PMC treatments included in the study, the clinical success rate was 98.2%. Conclusions: The clinical success rate of primary teeth treated with pulpotomy + IRM + PMCs was high, close to 100%, based on evidence and symptom reports from parents/caregivers. It would be beneficial to implement its use in public health institutions in countries such as Mexico. [ABSTRACT FROM AUTHOR]

New material of the small raoellid artiodactyl Metkatius kashmiriensis is reported from the middle Eocene of the Upper Subathu Formation in the Kalakot area, Jammu and Kashmir, northwest Himalaya, India. The fossil material consists of numerous mandibular and maxillary fragments and isolated teeth, mainly belonging to juvenile specimens. It documents the poorly known dental morphology of M. kashmiriensis and provides an overview of its intraspecific variation, allowing to redefine its diagnosis. M. kashmiriensis is characterized by a particularly small size compared with other raoellid species, and by bunodont molars with moderately marked transverse lophs. The M/1–2 are much longer than wide and display characters similar to those of Rajouria gunnelli , such as the presence of a small paraconid and a mesial mesiostylid. The P/4 bears distally a small hypoconid, which appears to be unique in Raoellidae. The description of the new material also allows to document the poorly known morphology of the deciduous teeth of raoellids. The DP2/ is reported for the first time, and the DP/4 of M. kashmiriensis shows a morphology different from that of Indohyus , with the absence of mesial basin anterior to the paraconid and the primoconid. Contrary to what has recently been proposed, these results confirm that M. kashmiriensis is a valid species and not a synonym of Indohyus indirae , and highlight the great morphological diversity present within the Raoellidae during the middle Eocene in the Indian subcontinent. [ABSTRACT FROM AUTHOR]

Quercetin is a natural flavonoid renowned for its potent antioxidant, anti-inflammatory, anti-diabetic, and antibacterial properties, making it a highly promising candidate for the treatment of various medical conditions. Our current study investigates the influence of quercetin on energy metabolism, fatty acid composition, oxidative stress gene expression, and sirtuin expression in early- and late-stage passages of stem cells derived from human exfoliated deciduous teeth (SHEDs). Mitochondrial respiration was analyzed by measuring oxygen consumption following a 24 h quercetin treatment, while fatty acid profiles were examined using gas chromatography–mass spectrometry (GC-MS). Additionally, quantitative PCR (qPCR) was used to assess the expression of oxidative stress genes and sirtuins. In younger SHEDs, quercetin enhances metabolic activity and mitochondrial respiration, although higher doses may decrease mitochondrial activity. Conversely, in older, senescent SHEDs, quercetin supports mitochondrial function at lower concentrations but appears to inhibit respiration at higher doses. These results suggest that quercetin may hold therapeutic potential for maintaining SHED viability and function, especially at lower doses in older cells. Further research is essential to fully elucidate a dose-dependent effect of quercetin and optimize its applications in regenerative medicine. [ABSTRACT FROM AUTHOR]

Background/Objectives: The development of new materials incorporating bioactive molecules for tissue regeneration is a growing area of interest. The objective of this study was to develop a new complex specifically designed for bone and skin tissue engineering, combining chitosan, ascorbic acid-2-magnesium phosphate (ASAP), and β-tricalcium phosphate (β-TCP). Methods: Chitosan and the complexes chitosan/ASAP and chitosan/ASAP/β-TCP were prepared in membrane form, macerated to a particulate format, and then subjected to characterization through Fourier transform infrared (FTIR) spectroscopy, optical and scanning electron microscopy (SEM), zeta potential, thermogravimetric analysis (TGA), and differential scanning calorimetry (DSC). Cell viability was evaluated through a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay and with fluorescein diacetate (FDA) and propidium iodide (PI) staining in stem cells obtained from deciduous teeth. Statistical analyses were performed using analysis of variance (ANOVA), followed by Tukey's test. Results: The FTIR results indicated the characteristic bands in the chitosan group and the complexation between chitosan, ASAP, and β-TCP. Microscopic characterization revealed a polydisperse distribution of micrometric particles. Zeta potential measurements demonstrated a reduction in surface charge upon the addition of ASAP and β-TCP to the chitosan matrix. TGA and DSC analyses further indicated complexation between the three components and the successful formation of a cross-linked structure in the chitosan matrix. Stem cells cultured with the particulate biomaterials demonstrated their biocompatibility. Statistical analysis revealed a significant increase in cell viability for the chitosan/ASAP and chitosan/ASAP/β-TCP groups compared to the chitosan control. Conclusions: Therefore, the chitosan/ASAP complex demonstrated potential for skin regeneration, while the chitosan/ASAP/β-TCP formulation showed promise as a biomaterial for bone regeneration due to the presence of β-tricalcium phosphate. [ABSTRACT FROM AUTHOR]

The article in the journal "Dental Traumatology" discusses the importance of public health policies, regenerative endodontics, autotransplantation, computer-aided autotransplantation, tooth apexification, fragment reattachment, maxillofacial trauma in pediatric populations, restoration techniques for endodontically treated teeth, mouthguards in sports, social media's role in disseminating knowledge about dental trauma, and the integration of artificial intelligence in dental care. The text emphasizes the need for evidence-based practices, continued research, and advancements in dental trauma management to enhance patient outcomes and improve long-term dental health. The authors highlight the significance of accurate information dissemination, standardized treatment protocols, and the potential benefits of innovative techniques in dental care. [Extracted from the article]

Background: The aim of this study was to calculate the stress distribution of fiberglass post associated with resin composite crown restoration and fiberglass posts with zirconia restorations in mature and immature endodontically treated central maxillary incisor under various loading conditions. Materials and Methods: The study created six different study models in a virtual environment: healthy mature maxillary central teeth, intact immature maxillary central teeth, mature maxillary central teeth with fiberglass post associated with resin composite crown restoration, immature maxillary central teeth with fiberglass post associated with resin composite crown restoration, mature maxillary central teeth with fiberglass posts and zirconia restoration, and immature maxillary central teeth with fiberglass posts and zirconia restoration. Loading conditions simulating mastication, trauma, and bruxism were applied to each of the models at different angles and amounts. The von Mises and the maximum and minimum principal stress values in tooth structures (dentin) and support structures (bone, PDL) and materials were observed using finite element stress analysis. Results: The highest stress values in the tissue and the restoration structure were observed for masticating force and crowns rehabilitated with zirconia restorations. None of the compared loading conditions and restorations showed destructive stress values on periodontal ligament or bone. Conclusion: The mature and immature endodontically treated central maxillary incisors can be better rehabilitated using fiberglass post associated with resin composite crown restoration and may be preferred to zirconia restorations in order to reduce the stresses on the surrounding tissues and teeth. However, further clinical studies are needed to fully explore this topic. [ABSTRACT FROM AUTHOR]

Traumatic dental injuries (TDI) are common in children due learning to walk and lack of balance that leads to falls. Luxation is the trauma that occurs most frequently in the deciduous, being that the intrusive and the avulsive are the ones that cause more damage to the permanent successors. The potential to cause disturbances to the developing permanent germ is high due to anatomical proximity and depends on age, direction of intrusion, severity and treatment. The consequences to the permanent range from hypocalcifications of enamel to retention of the permanent germ. In this case, the developmental disturbance of the tooth 21 presenting with acute dentoalveolar abscess was a result of a three‐degree intrusive luxation of the deciduous predecessor. The tooth 61 was misdiagnosed initially as avulsion, but it was a total intrusion as uncovered after a radiographic examination that showed an image suggestive of the presence of the deciduous tooth. The deciduous was extracted along with his permanent successor through outpatient procedure under antibiotic coverage and local anesthesia. The macro and microscopic analysis of the piece evidenced the presence of elements 21 and 61 closely united, as well as alterations provoked in both. There was remission of the infectious process and after 7 days it was verified the correct healing of the surgical wound. The radical outcome of this case emphasizes the relevance of appropriate clinical support as soon as possible in all TDI. [ABSTRACT FROM AUTHOR]

The dentition is critical to animal survival and teeth are present in modern vertebrates including teleost fish, sharks, amphibians, mammals and reptiles. The developmental processes that give rise to teeth are not just preserved through evolution but also share high level of similarity with the embryogenesis of other ectodermal organs. In this review we go beyond the embryonic phase of tooth development to life-long tooth replacement. We will address the origins of successional teeth, the location of putative tissue-resident stem cells, how de novo tooth formation continues throughout life and how teeth are shed in a spatially and temporally controlled manner. We review the evidence that the dental epithelium, which is the earliest recognizable dental structure in the reptilian dentition, serves as a putative niche for tissue-resident epithelial stem cells and recent molecular findings from transcriptomics carried out in reptilian dentitions. We discuss how odontoclasts resorb the primary tooth allowing eruption of the successional tooth. The reptiles, particularly lizards, are emerging as some of the most accessible animals to study tooth replacement which has relevance to evolution of the dentition and human dental disorders. [Display omitted] • A review of the common origins of teeth and other ectodermal specializations. • Introduction to adult reptile teeth which undergo life-long organ regeneration. • Review of RNA expression profiling of reptilian dentitions and insights into the molecular mechanisms of tooth replacement. • A review of pulse-chase experiments to define the locations of tissue-resident, dental epithelial stem cells. • How comparative studies on reptile teeth contribute to the fields of evolution, organ replacement, and dental pathology. [ABSTRACT FROM AUTHOR]