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ROS-mediated cross-talk between mitochondria and lysosomes can be visualized using rhodamine–phenothiazine conjugates.

The mitochondria and endoplasmic reticulum (ER) are highly dynamic subcellular structures essential for several biological functions. The development of non-toxic, wash-free fluorophores to visualize these structures inside cells aid in understanding their localization and dynamics in diverse cellular processes. In this paper, we report the synthesis and characterization of lutidine-based cationic fluorophores bearing push-pull substituents exhibiting emission in green and red wavelength regions and their subcellular localization in living cells. The confocal imaging of the molecules in a cellular domain reveals the robust localization of three molecules (2, 4 and 5) in the mitochondria and two molecules with polyfluorophenyl substituents (6 and 7) in the ER. At the same time, the other two molecules (1 and 3) showed non-specific imaging. These molecules can also be used to sense the altered viscosity of the stressed ER and investigate its dynamics.

Trifluoromethyl-substituted fluorophores with naphthalene and julolidine groups were utilized imaging and quantifying lipid droplets in COS-7 cells.

Purpose “Machine learning (ML)” in business aids in increasing company scalability and boosting company operations for businesses all over the world. “Artificial intelligence (AI)” technologies and several “ML” algorithms have grown in prominence in the business analytics sector. In the era of a huge quantum of data being generated by the virtue of the integration of the various software with the business operations, the relevance of “ML” is continuously increasing. As a result, companies may now profit from knowing how companies may use “ML” and incorporating it into their own operations. “ML” derives useful results from the data to address very dynamic and difficult social and business problems. ML helps in establishing a system that learns automatically and produces results in less time and effort, allowing machines to discover. ML is developing at a breakneck pace, fuelled mostly by new computer technology to competitive advantages during the COVID pandemic. Design/methodology/approach For firms all around the world, “ML” in business aids in expanding scalability and boosting operations. In the field of business analytics, artificial intelligence (AI) and machine learning (ML) algorithms have become increasingly popular. The importance of “ML” is growing in an era when a massive amount of data is generated as a result of the integration of various applications with company activities. As a result, businesses can now benefit from understanding how other businesses are using “ML” and adopting it into their own operations. In order to handle very dynamic and demanding societal and business challenges, machine learning (ML) extracts valuable results from data. Machine learning (ML) aids in the development of a system that learns automatically and generates outcomes with less time and effort, allowing machines to discover. ML is progressing at a dizzying pace, fueled primarily by new computer technology and used to gain competitive advantages during the COVID pandemic. Findings According to a new study published by the Accenture Institute for High Performance, “AI” might double yearly economic growth rates in several wealthy nations by 2035. With broad AI deployment, the yearly growth rate in the USA increased from 2.6% to 4.6%, resulting in an extra $8.3tn. In the UK, AI may contribute $814bn to the economy, raising the yearly growth rate from 2.5% to 3.9%. The authors are already in a business period when huge technological development is assisting us in addressing a variety of difficulties to achieve maximum development. AI technology has enormous developmental consequences. In addition, big data analytics is helping to make AI more enterprise ready. Future developments in “ML” cannot be understated. Machines will very certainly eventually be smarter than humans in practically every way. Originality/value The introduction of AI into the market has enabled small businesses to use tried-and-true strategies for achieving greater business objectives. AI is continually offering a competitive advantage to start-ups, whilst large corporations provide a platform for building novel solutions. AI has become an integral component of reality, from functioning as a robot in a production unit to self-driving automobiles and voice activated resources in complex medical procedures. As a consequence, solving the difficulties highlighted below and finding out how to collaborate with robots will be a constant problem for the human species (Sujaya and Bhaskar, 2021).

Fluorescent probes offer incredibly effective tools for visualizing the dynamic morphology of lipid droplets (LDs) and investigating their physiological interactions. In this work, we have utilized solvatochromic coumarin probes bearing nitrile and ester substituents for live-cell imaging. The fluorescence probes are characterized by a donor (diethylamino) and acceptor (nitrile and/or ester) substituents and a rotatable double bond. The designed architecture allows investigation of environmental sensitivity apart from providing excellent ability to target sub-cellular organelles. The synthesized fluorophores showed low cytotoxicity and excellent localization within the lipid droplets. Further, the fluorophores were also utilized to study viscosity changes within the LDs induced by Nystatin. More importantly, we also demonstrate imaging of LDs in multi-cellular animal models such as C. elegans.

Advances in developing organic fluorescent probes and fluorescence imaging techniques have enhanced our understanding of cell biology. The endoplasmic reticulum (ER) is a dynamic structure that plays a crucial role in protein synthesis, post-translational modifications, and lipid metabolism. The malfunction of ER contributes to several physiological and pathological conditions. Therefore, the investigations on the imaging and role of ER have attracted a lot of attention. Due to their simplicity, synthetic tunability, photostability, high quantum yields, easier cellular uptake, and lower cytotoxicity, organic fluorophores offer invaluable tools for the precision targeting of various cellular organelles and probe ER dynamics. The precision staining is made possible by incorporating specific functional groups having preferential and local organelle biomolecular interactions. For instance, functional moieties such as methyl sulfonamide, sulfonylurea, and pentafluorophenyl assist in ER targeting and thus have become essential tools to probe a deeper understanding of their dynamics. Furthermore, dual-function fluorescent probes that simultaneously image ER and detect specific physiological parameters or biological analytes were achieved by introducing special recognition or chemically reactive sites. This article attempts to comprehensively capture various design strategies currently employed by researchers utilizing small organic molecules to target the ER and detect specific analytes.

Researchers have mentioned the importance of digitization in improving efficiency and productivity in Small and Medium Enterprises (SME). Fortunately, there is no proof that Digitization can be used to deal with the outcome of severe incidents like COVID-19. The research paper suggested that the increased rate of SMEs has increased significantly. This was entirely due to the advent of Digital Technology (DT). In this way, both product and the process become more automated in digitalization, resulting in increased quality and demand. Considering the high scope for higher development, India's SME sector still has much space for new digital technologies to be integrated. This paper addresses the main scenario of SMEs in India and their benefit in GDP. Also, the research includes a brief analysis of CRM applications and digital payment options in SMEs.

Waste management is one of the world's biggest challenges, either in the developed or the emerging economies. The biggest problem with pollution is that the compost heap is well flowed in public areas before the next sanitation period starts. Demographic expansion has caused the hygienic condition with regard to the waste management system to deteriorate considerably. Disposal of waste is a fundamental element in waste disposal. Gradually, the technologies of artificial intelligence (AI) gained popularity in offering different computer ways to solving intelligent waste problem. The management of misdefined issues, experiences and uncertainties and partial data were efficient for AI. Even though this work did conduct much study, very few evaluations demonstrated the influence of AI to resolve many difficulties of intelligent management of waste. Accurate evaluation of garbage amount and quality is critical to Smart waste management system development and design. However, it is a challenging task to anticipate the quantity of trash created, given the several characteristics and its variability. The framework utilized in this document is the convolution neural network, a suitable approach for estimating the waste mass.

The purpose of this study is to establish responsibilities, policies, and procedures for the Human Resources Development of BHEL: HERP VARANASI. This study examines the benefits, the satisfaction regarding training programs, and improvements in training and development of employees in an organization. The focus of current study is to understand the affect of Training and Development. The major finding of the study indicated that training and development of employees increases organizational effectiveness and enhances competitiveness. This study projects that how training and development practices could enhance to improve organizational effectiveness and retain human capital. An introduction to Training and Development Training refers to the instructions provided to increase the knowledge and skill for the current job. An educated and well-trained work force is considered to be essential to the maintenance of a business firm’s competitive advantage in a global economy. These activities may occur in short burst or be over a longer period of time. The employee performance depends on various factors. But the most important factor of employee performance is training. Training is important to enhance the capabilities of employees. Now a day’s training is the most important factor in the business world because training increases the efficiency and the effectiveness of both employees and the organization. Training & development refers to any activities at all through which managers and workers improve their work-related skills and knowledge. Training is considered as the process of upgrading the knowledge, developing skills, bringing about attitude and behavioral changes, and improving the ability of the trainee to perform tasks effectively and efficiently in organizations (Wills, 1994; Palo et al, 2003; Robert et al,2004). BHEL: HERP VARANASI (Heavy Equipment Repair Plant) – An overview HERP is located at Shivpur, 11 Kms from main railway station and 15 Kms from Varanasi Airport. HERP is also situated at the center of the largest power belt of northern region. This power belt supplies 10650 MW of power to the country. In the line with BHEL’s of providing constant service at their doorsteps, the idea of establishing repair shop in the vicinity of power station was mooted objective. Accordingly, two repair plants at Bombay & Varanasi came into existence; the foundation equipment repair plant sprawling in 29.8 acre area at Varanasi was laid on 20th September 1984 by Chief Minister of U.P. Shri Narayan Dutt Tiwari within a short span of 21 month much before the schedule. Starting a manufacturer of OM Harzing, 2004). workforce and retention of the existing staff. Similarly, Echard and Berge (2008) stated that effective training techniques can produce significant business results especially in customer service, product development, and capability in obtaining new skill set. It is very necessary for the organization to design the training very carefully (Michael Armstrong,2000). The design of the training should be according to the needs of the employees (Ginsberg, 1997). It seems that Training design plays a very vital role in the employee as well as organizational performance. a bad training design is nothing but the loss of time and money (Tsaur and Lin, 2004). It is very difficult for an employee to perform well at the job place without any pre-training (Thomas N. Garavan, 1997). Trained employees perform well as compared to untrained employees (Partlow, 1996; Tihanyi et al., 2000; Boudreau et al.,2001). Every organization should develop its employees according to the need of that time so that they could compete with their competitors (Carlos A. Primo Braga,1995). Objectives of this paper • To study the Training & Development in BHEL: HERP VARANSI • To study the benefits of training in BHEL: HERP VARANSI Research methodology The study is based on primary data and the information is collected by supplying the questionnaire as well as interviewing the respondents. There were total 135 non-executives in the study out of which 116 were provided information and remaining were either absent or refused to respond. The analysis of the questionnaire was undertaken using Statistical Package for Social Sciences (SPSS).

Withania somnifera is one of the important medicinal plant grown for its root. The present experiment was carried out to evaluate the most efficient bioinoculants as a seed treatment for the growth and yield of W. somnifera under pot conditions. The results revealed that N-fixer Bacillus sp. (AZHGF1) strain was most efficient microbe for promoting growth and yield of W.somnifera. In the tested arbuscular mycorrhizal (AM) fungi, we have demonstrated that Glomus intraradices (GI) was the potential AM fungus for the better growth and root yield of W. somnifera. The rhizospheric population of bioinoculants was maintained which may be useful for the next cropping season plants. This technology may be adopted in many seed borne crops which may be useful in safe and sound management of soil health.

The effects of aqueous leachate of Nicotiana plumbaginifolia Viv. on germination, seedling growth, amylase activity, sugar and starch contents of germinated seeds of maize (Zea mays L. cv. Uttam) were examined. Effects of leachate on photosynthetic pigments, protein content, activities of nitrate reductase and some antioxidants were also studied. Higher concentration of aqueous leachate of N. plumbaginifolia reduced the germination rate (GR). However, final germination percentage remained almost unaffected. Lower concentration of leachate stimulated the amylase activity and resulted in higher sugar content and GR. The increasing concentrations of leachate inhibited the conversion of starch into sugars. Allelochemicals decreased the amount of chlorophyll a, chlorophyll b, carotenoids, protein and nitrate reductase activity (NRA). The leachate of lower concentrations stimulated the activity of peroxidase but slight decrease was recorded in higher concentration. Superoxide dismutase and catalase exhibited concentration dependent increase except in seedlings treated with 100% concentration of leachate. Impairment of various metabolic activities due to leachate resulted in decreased root and shoot length.

The sunflower (Helianthus annuus L. cv. PAC 36) seedlings were inoculated with plant growth promoting rhizobacteria (PGPR), viz. Azotobacter chroococcum (A+), Bacillus polymyxa (B+), separately and in combination of the two (AB+). Relative water content and seedling growth were maximum in AB+ seedlings under control. Water stress significantly decreased the RWC, growth and dry mass of non-inoculated seedlings. However, inoculated seedlings maintained higher growth even under water stress. Pigments and protein contents decreased under water stress, but higher amount of the same was observed in stressed AB+ seedlings. Enhanced activity of nitrate reductase was recorded in AB+ seedlings with maximum in control. Water stress significantly decreased the nitrate reductase activity. A significant increase in the activity of superoxide dismutase (SOD) in leaves was recorded under water stress except in B+ with maximum increase in non-inoculated seedlings. Catalase (CAT) activity decreased in stressed non-inoculated seedlings while increased in the leaves of A+ and AB+ seedlings. Almost similar trends were recorded for both leaves and cotyledons. PGPR improved the water status in stressed seedlings and thereby physiological and biochemical parameters and thus ameliorated the severe effects of water stress.

Medicinal coleus (Coleus forskohlii) is an important medicinal crop which contains forskolin in their roots. Because of continuous collection of roots from the wild sources, this plant has been included in the list of endangered species. The crop has a great potential in future due to the expected increase in demand for forskolin which is widely used in glaucoma, cardiac problems, eczema, asthma, and hypertension and also used in the treatment of certain types of cancers. With the present annual production of about 100 tons from 700 ha in India, cultivation of C. forskohlii is picking up because of its economic potential. However, the crop has not become very popular among farmers because of its susceptibility to many diseases of which root- rot/wilt is the most important, causing serious losses affecting the tuber yield. The present review focuses on various diseases of important medicinal plant C. forskohlii and their management. [Medicinal Plants 2011; 3(1) : 1-7].

Nodal and shoot tip explants were collected from field grown mature plants of pointed gourd (T. dioica) for initiation of shoot cultures. The sterilized explants were aseptically inoculated on MS medium supplemented with various concentrations of BAP and kinetin alone and in combinations. Observations were made on 15 explants showing shoot initiation, average length of shoot and number of days taken by nodal/shoot segments to initiate the shoots. Medium containing BAP (1mg/ml) proved better for establishment of nodal explants. Out of 15, 12 explants established successfully on this medium. The growth of shoots was also found better than on other media tried. It took minimum time (5–6 days) to initiate the culture and the length of the shoot was ~ 1.5 cm with a survival of 80%. BAP at a concentration of 1mg/ml was found most suitable for shoot initiation from nodal explants of T. dioica in the present study.