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1. Changes in upper airways microbiota in ventilator-associated pneumonia

Author

Alagna, Laura, Mancabelli, Leonardo, Magni, Federico, Chatenoud, Liliane, Bassi, Gabriele, Del Bianco, Silvia, Fumagalli, Roberto, Turroni, Francesca, Mangioni, Davide, Migliorino, Guglielmo M., Milani, Christian, Muscatello, Antonio, Nattino, Giovanni, Picetti, Edoardo, Pinciroli, Riccardo, Rossi, Sandra, Tonetti, Tommaso, Vargiolu, Alessia, Bandera, Alessandra, Ventura, Marco, Citerio, Giuseppe, and Gori, Andrea

Published
2023
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2. The Neurological Pupil index for outcome prognostication in people with acute brain injury (ORANGE): a prospective, observational, multicentre cohort study

Author

Abed-Maillard, Samia, Anderloni, Marco, Beretta, Alessandra, Cho, Sung-Min, Del Bianco, Silvia, Favre, Eva, Greil, Madeline E., Guglielmi, Angelo, Higuera Lucas, Juan, Iacca, Cosimo, Kuramatsu, Joji B., Lundberg, Linda Marie, Magni, Federico, Malgeri, Letterio, Mangili, Paolo, Melchionda, Isabella, Miroz, John-Paul, Monleón, Berta, Randazzo, Dominica, Salah, Samia, Scavone, Angela, Schilte, Clothilde, Silva, Serena, Sunde, Kjetil, Wang, Ruihao, Oddo, Mauro, Taccone, Fabio S, Petrosino, Matteo, Badenes, Rafael, Blandino-Ortiz, Aaron, Bouzat, Pierre, Caricato, Anselmo, Chesnut, Randall M, Feyling, Anders C, Ben-Hamouda, Nawfel, Hemphill, J Claude, Koehn, Julia, Rasulo, Frank, Suarez, Jose I, Elli, Francesca, Vargiolu, Alessia, Rebora, Paola, Galimberti, Stefania, and Citerio, Giuseppe

Published
2023
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4. The Neurological Pupil index for outcome prognostication in people with acute brain injury (ORANGE): a prospective, observational, multicentre cohort study

Author

Oddo, Mauro, primary, Taccone, Fabio S, additional, Petrosino, Matteo, additional, Badenes, Rafael, additional, Blandino-Ortiz, Aaron, additional, Bouzat, Pierre, additional, Caricato, Anselmo, additional, Chesnut, Randall M, additional, Feyling, Anders C, additional, Ben-Hamouda, Nawfel, additional, Hemphill, J Claude, additional, Koehn, Julia, additional, Rasulo, Frank, additional, Suarez, Jose I, additional, Elli, Francesca, additional, Vargiolu, Alessia, additional, Rebora, Paola, additional, Galimberti, Stefania, additional, Citerio, Giuseppe, additional, Abed-Maillard, Samia, additional, Anderloni, Marco, additional, Beretta, Alessandra, additional, Cho, Sung-Min, additional, Del Bianco, Silvia, additional, Favre, Eva, additional, Greil, Madeline E., additional, Guglielmi, Angelo, additional, Higuera Lucas, Juan, additional, Iacca, Cosimo, additional, Kuramatsu, Joji B., additional, Lundberg, Linda Marie, additional, Magni, Federico, additional, Malgeri, Letterio, additional, Mangili, Paolo, additional, Melchionda, Isabella, additional, Miroz, John-Paul, additional, Monleón, Berta, additional, Randazzo, Dominica, additional, Salah, Samia, additional, Scavone, Angela, additional, Schilte, Clothilde, additional, Silva, Serena, additional, Sunde, Kjetil, additional, and Wang, Ruihao, additional

Published
2023
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5. The Neurological Pupil index for outcome prognostication in people with acute brain injury (ORANGE): a prospective, observational, multicentre cohort study

Author

Oddo, M, Taccone, F, Petrosino, M, Badenes, R, Blandino-Ortiz, A, Bouzat, P, Caricato, A, Chesnut, R, Feyling, A, Ben-Hamouda, N, Hemphill, J, Koehn, J, Rasulo, F, Suarez, J, Elli, F, Vargiolu, A, Rebora, P, Galimberti, S, Citerio, G, Abed-Maillard, S, Anderloni, M, Beretta, A, Cho, S, Del Bianco, S, Favre, E, Greil, M, Guglielmi, A, Higuera Lucas, J, Iacca, C, Kuramatsu, J, Lundberg, L, Magni, F, Malgeri, L, Mangili, P, Melchionda, I, Miroz, J, Monleón, B, Randazzo, D, Salah, S, Scavone, A, Schilte, C, Silva, S, Sunde, K, Wang, R, Oddo, Mauro, Taccone, Fabio S, Petrosino, Matteo, Badenes, Rafael, Blandino-Ortiz, Aaron, Bouzat, Pierre, Caricato, Anselmo, Chesnut, Randall M, Feyling, Anders C, Ben-Hamouda, Nawfel, Hemphill, J Claude, Koehn, Julia, Rasulo, Frank, Suarez, Jose I, Elli, Francesca, Vargiolu, Alessia, Rebora, Paola, Galimberti, Stefania, Citerio, Giuseppe, Abed-Maillard, Samia, Anderloni, Marco, Beretta, Alessandra, Cho, Sung-Min, Del Bianco, Silvia, Favre, Eva, Greil, Madeline E., Guglielmi, Angelo, Higuera Lucas, Juan, Iacca, Cosimo, Kuramatsu, Joji B., Lundberg, Linda Marie, Magni, Federico, Malgeri, Letterio, Mangili, Paolo, Melchionda, Isabella, Miroz, John-Paul, Monleón, Berta, Randazzo, Dominica, Salah, Samia, Scavone, Angela, Schilte, Clothilde, Silva, Serena, Sunde, Kjetil, Wang, Ruihao, Oddo, M, Taccone, F, Petrosino, M, Badenes, R, Blandino-Ortiz, A, Bouzat, P, Caricato, A, Chesnut, R, Feyling, A, Ben-Hamouda, N, Hemphill, J, Koehn, J, Rasulo, F, Suarez, J, Elli, F, Vargiolu, A, Rebora, P, Galimberti, S, Citerio, G, Abed-Maillard, S, Anderloni, M, Beretta, A, Cho, S, Del Bianco, S, Favre, E, Greil, M, Guglielmi, A, Higuera Lucas, J, Iacca, C, Kuramatsu, J, Lundberg, L, Magni, F, Malgeri, L, Mangili, P, Melchionda, I, Miroz, J, Monleón, B, Randazzo, D, Salah, S, Scavone, A, Schilte, C, Silva, S, Sunde, K, Wang, R, Oddo, Mauro, Taccone, Fabio S, Petrosino, Matteo, Badenes, Rafael, Blandino-Ortiz, Aaron, Bouzat, Pierre, Caricato, Anselmo, Chesnut, Randall M, Feyling, Anders C, Ben-Hamouda, Nawfel, Hemphill, J Claude, Koehn, Julia, Rasulo, Frank, Suarez, Jose I, Elli, Francesca, Vargiolu, Alessia, Rebora, Paola, Galimberti, Stefania, Citerio, Giuseppe, Abed-Maillard, Samia, Anderloni, Marco, Beretta, Alessandra, Cho, Sung-Min, Del Bianco, Silvia, Favre, Eva, Greil, Madeline E., Guglielmi, Angelo, Higuera Lucas, Juan, Iacca, Cosimo, Kuramatsu, Joji B., Lundberg, Linda Marie, Magni, Federico, Malgeri, Letterio, Mangili, Paolo, Melchionda, Isabella, Miroz, John-Paul, Monleón, Berta, Randazzo, Dominica, Salah, Samia, Scavone, Angela, Schilte, Clothilde, Silva, Serena, Sunde, Kjetil, and Wang, Ruihao

Abstract

Background Improving the prognostication of acute brain injury is a key element of critical care. Standard assessment includes pupillary light reactivity testing with a hand-held light source, but findings are interpreted subjectively; automated pupillometry might be more precise and reproducible. We aimed to assess the association of the Neurological Pupil index (NPi)—a quantitative measure of pupillary reactivity computed by automated pupillometry— with outcomes of patients with severe non-anoxic acute brain injury. Methods ORANGE is a multicentre, prospective, observational cohort study at 13 hospitals in eight countries in Europe and North America. Patients admitted to the intensive care unit after traumatic brain injury, aneurysmal subarachnoid haemorrhage, or intracerebral haemorrhage were eligible for the study. Patients underwent automated infrared pupillometry assessment every 4 h during the first 7 days after admission to compute NPi, with values ranging from 0 to 5 (with abnormal NPi being <3). The co-primary outcomes of the study were neurological outcome (assessed with the extended Glasgow Outcome Scale [GOSE]) and mortality at 6 months. We used logistic regression to model the association between NPi and poor neurological outcome (GOSE ≤4) at 6 months and Cox regression to model the relation of NPi with 6-month mortality. This study is registered with ClinicalTrials.gov, NCT04490005. Findings Between Nov 1, 2020, and May 3, 2022, 514 patients (224 with traumatic brain injury, 139 with aneurysmal subarachnoid haemorrhage, and 151 with intracerebral haemorrhage) were enrolled. The median age of patients was 61 years (IQR 46–71), and the median Glasgow Coma Scale score on admission was 8 (5–11). 40 071 NPi measurements were taken (median 40 per patient [20–50]). The 6-month outcome was assessed in 497 (97%) patients, of whom 160 (32%) patients died, and 241 (47%) patients had at least one recording of abnormal NPi, which was associated with poor neurol

Published
2023

6. Changes in upper airways microbiota in ventilator-associated pneumonia

Author

Alagna, L, Mancabelli, L, Magni, F, Chatenoud, L, Bassi, G, Del Bianco, S, Fumagalli, R, Turroni, F, Mangioni, D, Migliorino, G, Milani, C, Muscatello, A, Nattino, G, Picetti, E, Pinciroli, R, Rossi, S, Tonetti, T, Vargiolu, A, Bandera, A, Ventura, M, Citerio, G, Gori, A, Alagna, Laura, Mancabelli, Leonardo, Magni, Federico, Chatenoud, Liliane, Bassi, Gabriele, Del Bianco, Silvia, Fumagalli, Roberto, Turroni, Francesca, Mangioni, Davide, Migliorino, Guglielmo M, Milani, Christian, Muscatello, Antonio, Nattino, Giovanni, Picetti, Edoardo, Pinciroli, Riccardo, Rossi, Sandra, Tonetti, Tommaso, Vargiolu, Alessia, Bandera, Alessandra, Ventura, Marco, Citerio, Giuseppe, Gori, Andrea, Alagna, L, Mancabelli, L, Magni, F, Chatenoud, L, Bassi, G, Del Bianco, S, Fumagalli, R, Turroni, F, Mangioni, D, Migliorino, G, Milani, C, Muscatello, A, Nattino, G, Picetti, E, Pinciroli, R, Rossi, S, Tonetti, T, Vargiolu, A, Bandera, A, Ventura, M, Citerio, G, Gori, A, Alagna, Laura, Mancabelli, Leonardo, Magni, Federico, Chatenoud, Liliane, Bassi, Gabriele, Del Bianco, Silvia, Fumagalli, Roberto, Turroni, Francesca, Mangioni, Davide, Migliorino, Guglielmo M, Milani, Christian, Muscatello, Antonio, Nattino, Giovanni, Picetti, Edoardo, Pinciroli, Riccardo, Rossi, Sandra, Tonetti, Tommaso, Vargiolu, Alessia, Bandera, Alessandra, Ventura, Marco, Citerio, Giuseppe, and Gori, Andrea

Abstract

Background: The role of upper airways microbiota and its association with ventilator-associated pneumonia (VAP) development in mechanically ventilated (MV) patients is unclear. Taking advantage of data collected in a prospective study aimed to assess the composition and over-time variation of upper airway microbiota in patients MV for non-pulmonary reasons, we describe upper airway microbiota characteristics among VAP and NO-VAP patients. Methods: Exploratory analysis of data collected in a prospective observational study on patients intubated for non-pulmonary conditions. Microbiota analysis (trough 16S-rRNA gene profiling) was performed on endotracheal aspirates (at intubation, T0, and after 72 h, T3) of patients with VAP (cases cohort) and a subgroup of NO-VAP patients (control cohort, matched according to total intubation time). Results: Samples from 13 VAP patients and 22 NO-VAP matched controls were analyzed. At intubation (T0), patients with VAP revealed a significantly lower microbial complexity of the microbiota of the upper airways compared to NO-VAP controls (alpha diversity index of 84 ± 37 and 160 ± 102, in VAP and NO_VAP group, respectively, p-value < 0.012). Furthermore, an overall decrease in microbial diversity was observed in both groups at T3 as compared to T0. At T3, a loss of some genera (Prevotella 7, Fusobacterium, Neisseria, Escherichia–Shigella and Haemophilus) was found in VAP patients. In contrast, eight genera belonging to the Bacteroidetes, Firmicutes and Fusobacteria phyla was predominant in this group. However, it is unclear whether VAP caused dysbiosis or dysbiosis caused VAP. Conclusions: In a small sample size of intubated patients, microbial diversity at intubation was less in patients with VAP compared to patients without VAP.

Published
2023

8. Influence of dietary inclusion of tannin extracts from mimosa, chestnut and tara on volatile compounds and flavour in lamb meat

Author

Del Bianco, Silvia, primary, Natalello, Antonio, additional, Luciano, Giuseppe, additional, Valenti, Bernardo, additional, Campidonico, Luca, additional, Gkarane, Vasiliki, additional, Monahan, Frank, additional, Biondi, Luisa, additional, Favotto, Saida, additional, Sepulcri, Angela, additional, and Piasentier, Edi, additional

Published
2021
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10. Eufonía : didáctica de la música

Author

del Bianco, Silvia

Subjects
rítmica, movimiento, educación musical, expresión corporal, vejez
Abstract

Resumen basado en el de la publicación Se describe como el movimiento corporal en relación con la música y en particular con los ejercicios multitareas, ejecutados al ritmo de la música improvisada en el piano, son la clave del éxito de la rítmica Jaques-Dalcroze. La interacción entre los participantes y la integración dentro del grupo completan los beneficios de esta actividad. En este artículo, se propone su aplicación en colectivos de edad avanzada, los séniores. Biblioteca de Educación del Ministerio de Educación y Formación Profesional; Calle San Agustín, 5 - 3 Planta; 28014 Madrid; Tel. +34917748000; biblioteca@mecd.es ESP

Published
2016

11. High-risk HPV and Chlamydia trachomatis co-infections in women with cervical dysplasia

Author

DEL BIANCO, SILVIA, MADEO, MOIRA, PIROVANO, CECILIA, CAREDDU, ANNA MARIA LAURA, MUSUMECI, ROSARIO, COCUZZA, CLEMENTINA ELVEZIA, Epifano, I, Dell'Anna, T, Salerio, F, DEL BIANCO, S, Madeo, M, Epifano, I, Dell'Anna, T, Pirovano, C, Salerio, F, Careddu, A, Musumeci, R, and Cocuzza, C

Subjects
MED/07 - MICROBIOLOGIA E MICROBIOLOGIA CLINICA, HR-HPV, Chlamydia trachomatis, cervical displasia
Published
2013

12. Eufonía : didáctica de la música

Author

del Bianco, Silvia

Subjects
rítmica, método de enseñanza, corriente pedagógica, Jaques-Dalcroze, Émile, educación musical, improvisación, expresión corporal, sonido
Abstract

Resumen basado en el de la publicación Título, resumen y palabras clave en español e inglés Se presenta la rítmica de Jaques-Dalcroze, un método que desarrolla la musicalidad a través del movimiento corporal, basándose en las estrechas relaciones entre el trabajo corporal consciente y el fenómeno sonoro. El movimiento (tanto el desplazamiento como la improvisación corporal) y la sensibilidad hacia la tensión y relajación muscular crean imágenes sensoriales (musculares y visuales) que permiten tomar consciencia de los elementos musicales tanto los relacionados con la métrica o el ritmo, como los ligados a la frase o a la forma, intensificando la vivencia de la música. Para ello es fundamental el aporte de la improvisación musical del profesor que permite que cada actividad sea acompañada con la música adecuada a la misma. Biblioteca de Educación del Ministerio de Educación y Formación Profesional; Calle San Agustín, 5 - 3 Planta; 28014 Madrid; Tel. +34917748000; biblioteca@mecd.es ESP

Published
2009

13. High-risk HPV and Chlamydia trachomatis co-infections in women with cervical dysplasia

Author

DEL BIANCO, S, Madeo, M, Epifano, I, Dell'Anna, T, Pirovano, C, Salerio, F, Careddu, A, Musumeci, R, Cocuzza, C, DEL BIANCO, SILVIA, MADEO, MOIRA, PIROVANO, CECILIA, CAREDDU, ANNA MARIA LAURA, MUSUMECI, ROSARIO, COCUZZA, CLEMENTINA ELVEZIA, DEL BIANCO, S, Madeo, M, Epifano, I, Dell'Anna, T, Pirovano, C, Salerio, F, Careddu, A, Musumeci, R, Cocuzza, C, DEL BIANCO, SILVIA, MADEO, MOIRA, PIROVANO, CECILIA, CAREDDU, ANNA MARIA LAURA, MUSUMECI, ROSARIO, and COCUZZA, CLEMENTINA ELVEZIA

Published
2013

14. Changes in upper airways microbiota in ventilator-associated pneumonia

Author

Laura Alagna, Leonardo Mancabelli, Federico Magni, Liliane Chatenoud, Gabriele Bassi, Silvia Del Bianco, Roberto Fumagalli, Francesca Turroni, Davide Mangioni, Guglielmo M. Migliorino, Christian Milani, Antonio Muscatello, Giovanni Nattino, Edoardo Picetti, Riccardo Pinciroli, Sandra Rossi, Tommaso Tonetti, Alessia Vargiolu, Alessandra Bandera, Marco Ventura, Giuseppe Citerio, Andrea Gori, Alagna, L, Mancabelli, L, Magni, F, Chatenoud, L, Bassi, G, Del Bianco, S, Fumagalli, R, Turroni, F, Mangioni, D, Migliorino, G, Milani, C, Muscatello, A, Nattino, G, Picetti, E, Pinciroli, R, Rossi, S, Tonetti, T, Vargiolu, A, Bandera, A, Ventura, M, Citerio, G, Gori, A, Alagna, Laura, Mancabelli, Leonardo, Magni, Federico, Chatenoud, Liliane, Bassi, Gabriele, Del Bianco, Silvia, Fumagalli, Roberto, Turroni, Francesca, Mangioni, Davide, Migliorino, Guglielmo M, Milani, Christian, Muscatello, Antonio, Nattino, Giovanni, Picetti, Edoardo, Pinciroli, Riccardo, Rossi, Sandra, Tonetti, Tommaso, Vargiolu, Alessia, Bandera, Alessandra, Ventura, Marco, Citerio, Giuseppe, and Gori, Andrea

Subjects
Mechanical ventilation, VAP, Ventilator-associated pneumonia, 16S-rRNA microbial profiling, Critical Care and Intensive Care Medicine, Cohort study, Upper airways microbiota
Abstract

Background The role of upper airways microbiota and its association with ventilator-associated pneumonia (VAP) development in mechanically ventilated (MV) patients is unclear. Taking advantage of data collected in a prospective study aimed to assess the composition and over-time variation of upper airway microbiota in patients MV for non-pulmonary reasons, we describe upper airway microbiota characteristics among VAP and NO-VAP patients. Methods Exploratory analysis of data collected in a prospective observational study on patients intubated for non-pulmonary conditions. Microbiota analysis (trough 16S-rRNA gene profiling) was performed on endotracheal aspirates (at intubation, T0, and after 72 h, T3) of patients with VAP (cases cohort) and a subgroup of NO-VAP patients (control cohort, matched according to total intubation time). Results Samples from 13 VAP patients and 22 NO-VAP matched controls were analyzed. At intubation (T0), patients with VAP revealed a significantly lower microbial complexity of the microbiota of the upper airways compared to NO-VAP controls (alpha diversity index of 84 ± 37 and 160 ± 102, in VAP and NO_VAP group, respectively, p-value Prevotella 7, Fusobacterium, Neisseria, Escherichia–Shigella and Haemophilus) was found in VAP patients. In contrast, eight genera belonging to the Bacteroidetes, Firmicutes and Fusobacteria phyla was predominant in this group. However, it is unclear whether VAP caused dysbiosis or dysbiosis caused VAP. Conclusions In a small sample size of intubated patients, microbial diversity at intubation was less in patients with VAP compared to patients without VAP.

Published
2023

15. Single injection of platelet-rich plasma in a rat Achilles tendon tear model.

Author

Parafioriti A, Armiraglio E, Del Bianco S, Tibalt E, Oliva F, and Berardi AC

Abstract

The purpose of this study was to determine the efficacy of platelet-rich plasma (PRP) 1-injection during an Achilles tendon rat tear model. 80 male adult imbreded rats (Wistar Kyoto), underwent under surgical tendon rupture. 40 Animal (PRP group rats) were given a local injection with 0,25 mL of PRP, and 40 animal (control group) were given the same quantity of control solution. The rats were sacrified at 1, 2, 4 and 6 weeks (each time point, 20 rats of the each group) after surgical tear and tendon tissue was analysed by macroscopic aspect, histology, immunostaining and Real Time (RT)-PCR to evaluate tissue repair. PRP improved tendon remodelling by better coordination of the reconstructive process with earlier formation of tendon-like continuity only in the first week after surgery. However, after 2,4 and 6 weeks, Achilles tendons in the PRP group had no difference compared to the control group. Immunostaining and RT-PCR did not show any difference between PRP treated and untreated group. Based on these findings a single injection of PRP appear not useful for Achilles rat tendon tear.

Published
2011

16. Hemochromatosis gene mutations and iron metabolism in celiac disease.

Author

Barisani D, Ceroni S, Del Bianco S, Meneveri R, and Bardella MT

Subjects
Adult, Alleles, Celiac Disease diet therapy, Cohort Studies, Female, Ferritins blood, Gene Frequency, Glutens, HLA Antigens genetics, Hemochromatosis genetics, Hemochromatosis Protein, Hemoglobins metabolism, Humans, Iron blood, Male, Mutation, Celiac Disease genetics, Celiac Disease metabolism, Histocompatibility Antigens Class I genetics, Iron metabolism, Membrane Proteins genetics
Abstract

Background and Objectives: Iron deficiency anemia is a common manifestation of celiac disease, which may be due to genetic and environmental factors. HFE mutations, frequent in Caucasian populations, can cause increased intestinal iron absorption and thus could protect against the development of iron deficiency. The aim of this study was to evaluate the prevalence of HFE mutations and their effect on iron metabolism in Italian celiac patients at diagnosis and after a gluten-free diet., Design and Methods: C282Y and H63D mutations were assessed by polymerase chain reaction (PCR) and restriction enzyme digestion in 203 patients with celiac disease and in 206 controls. HLA alleles were determined by sequence-specific primers and PCR. Duodenal histology was graded using Marsh's classification, and iron parameters measured by standard techniques., Results: The frequency of the C282Y mutation was similar in celiac patients and controls (0.034 vs. 0.031); comparable frequencies were detected also for the H63D allele (0.170 vs. 0.136 in celiac patients and controls, respectively). Neither of the two HFE mutations affected iron indices in celiac patients at diagnosis, whereas a significant inverse correlation was detected between hemoglobin or ferritin and severity of histological damage (Marsh 3C or 3B vs. 3A, p<0.05 for both parameters). After a gluten-free diet, a slight increase in hemoglobin levels was observed in C282Y carriers as compared to controls, but only in female patients (p=0.044)., Interpretation and Conclusions: In Italian patients with untreated celiac disease, HFE mutations do not constitute a protective factor against the development of iron deficiency, which seems to be mainly determined by the severity of the intestinal lesions.

Published
2004

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