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13 results on '"Delmas, Jean-Michel"'

6. Impact of therapeutic treatment with [beta]-lactam on transfer of the [bla.sub.CTM-M-9] resistance gene from Salmonella enterica serovar Virchow to Escherichia coli in gnotobiotic rats

Author

Faure, Stephanie, Perrin-Guyomard, Agnes, Delmas, Jean-Michel, and Laurentie, Michel

Subjects
Cefixime -- Usage, Escherichia coli -- Genetic aspects, Genetic transformation -- Analysis, Plasmids -- Research, Salmonella enteritidis -- Genetic aspects, Intestines -- Microbiology, Intestines -- Research, Biological sciences
Abstract

The conjugative transfer of the plasmid carrying the [bla.sub.CTX-M-9] gene from Salmonella enterica serovar Virchow isolated from a chicken farm to a recipient Escherichia coli strain is evaluated in vitro and in axenic rats inoculated with both strains, with or without selective pressure due to therapeutic doses of cefixime. The estimates of in vitro transfer underestimated potential transfer in the digestive tract and therapeutic doses of cefixime have not increased the selection for transconjugants.

Published
2009

8. Impact of Therapeutic Treatment with β-Lactam on Transfer of the blaCTX-M-9 Resistance Gene from Salmonella enterica Serovar Virchow to Escherichia coli in Gnotobiotic Rats▿

Author

Faure, Stéphanie, Perrin-Guyomard, Agnès, Delmas, Jean-Michel, Laurentie, Michel, Laboratoire d'études et de recherches sur les médicaments vétérinaires et les désinfectants, and Agence Française de Sécurité Sanitaire des Aliments (AFSSA)

Subjects
antibiotic resistance, Gene Transfer, Horizontal, Public Health Microbiology, beta-Lactams, beta-Lactam Resistance, beta-Lactamases, [SDV.SP.MED]Life Sciences [q-bio]/Pharmaceutical sciences/Medication, gnotobiotic rat, antibioresistance, Escherichia coli, Animals, Germ-Free Life, gene, Salmonella Infections, Animal, Escherichia coli Proteins, transmission, Salmonella enterica, Anti-Bacterial Agents, Rats, transfert, Conjugation, Genetic, salmonella enterica virchow, genetic, beta-lactan, Chickens, Plasmids
Abstract

The conjugative transfer of the plasmid carrying the bla(CTX-M-9) gene from Salmonella enterica serovar Virchow isolated from a chicken farm to a recipient Escherichia coli strain was evaluated in vitro and in axenic rats inoculated with both strains, with or without selective pressure due to therapeutic doses of cefixime. The transfer of the bla(CTX-M-9) gene of S. enterica serovar Virchow to E. coli was confirmed in vitro, at a low frequency of 5.9 x 10(-8) transconjugants/donors. This transfer rate was higher in gnotobiotic rats and reached approximately 10(-5) transconjugants/donors without selective pressure. This frequency was not affected by the addition of therapeutic doses of cefixime. Thus, estimates of in vitro transfer underestimated potential transfer in the digestive tract, and therapeutic doses of cefixime did not increase the selection for transconjugants.

Published
2009

13. Impact of Therapeutic Treatment with β-Lactam on Transfer of the blaCTX-M-9 Resistance Gene from Salmonella enterica Serovar Virchow to Escherichia coli in Gnotobiotic Rats.

Author

Faure, Stéphanie, Perrin-Guyomard, Agnès, Delmas, Jean-Michel, and Laurentie, Michel

Subjects
*PLASMIDS, *SALMONELLA, *ESCHERICHIA coli, *GERMFREE animals, *RATS, *MOBILE genetic elements
Abstract

The conjugative transfer of the plasmid carrying the bIaCTX-M-9 gene from Salmonella enterica serovar Virchow isolated from a chicken farm to a recipient Escherichia coli strain was evaluated in vitro and in axenic rats inoculated with both strains, with or without selective pressure due to therapeutic doses of cefixime. The transfer of the blaCTX-M-9 gene of S. enterica serovar Virchow to E. coli was confirmed in vitro, at a low frequency of 5.9 × 10-8 transconjugants/donors. This transfer rate was higher in gnotobiotic rats and reached ~10-5 transconjugants/donors without selective pressure. This frequency was not affected by the addition of therapeutic doses of cefixime. Thus, estimates of in vitro transfer underestimated potential transfer in the digestive tract, and therapeutic doses of cefixime did not increase the selection for transconjugants. [ABSTRACT FROM AUTHOR]

Published
2009
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