18 results on '"Delrio S"'
Search Results
2. Association between preoperative evaluation with lung ultrasound and outcome in frail elderly patients undergoing orthopedic surgery for hip fractures: study protocol for an Italian multicenter observational prospective study (LUSHIP)
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Vetrugno, L., Boero, E., Bignami, E., Cortegiani, A., Raineri, S. M., Spadaro, S., Moro, F., D'Inca, S., D'Orlando, L., Agro, F. E., Bernardinetti, M., Forfori, F., Corradi, F., Pregnolato, S., Mosconi, M., Bellini, V., Franchi, F., Mongelli, P., Leonardi, S., Giuffrida, C., Tescione, M., Bruni, A., Garofalo, E., Longhini, F., Cammarota, G., De Robertis, E., Giglio, G., Urso, F., Bove, T., Mattuzzi, L., Federici, N., Delrio, S., Meroi, F., Flaibani, L., Zaghis, C., Orso, D., Tomasino, S., Dottore, B., Divella, M., Mussetta, S., Musso, G., Minunno, A., Barbero, C., Puppo, M., Saturno, F., Galvano, A. N., Ippolito, M., Massari, L., Bianconi, M., Caruso, G., Ragazzi, R., Volta, C. A., Mongodi, S., Mojoli, F., Riccone, F., Scolletta, S., Macheda, S., Vulcano, S., Cosco, G., Vadala, E., Taddei, E., Isirdi, A., Vetrugno, Luigi, Boero, Enrico, Bignami, Elena, Cortegiani, Andrea, Raineri, Santi Maurizio, Spadaro, Savino, Moro, Federico, D'Incà, Stefano, D'Orlando, Lori, Agrò, Felice Eugenio, Bernardinetti, Mattia, Forfori, Francesco, Corradi, Francesco, Pregnolato, Sandro, Mosconi, Mario, Bellini, Valentina, Franchi, Federico, Mongelli, Pierpaolo, Leonardi, Salvatore, Giuffrida, Clemente, Tescione, Marco, Bruni, Andrea, Garofalo, Eugenio, Longhini, Federico, Cammarota, Gianmaria, De Robertis, Edoardo, Giglio, Giuseppe, Urso, Felice, Bove, Tiziana, and Lisa Mattuzzi, Nicola Federici, Silvia Delrio, Francesco Meroi, Luca Flaibani, Clara Zaghis, Daniele Orso, Serena Tomasino, Bruno Dottore, Michele Divella, Sabrina Mussetta, Gaia Musso, Angela Minunno, Carlo Barbero, Mattia Puppo, Francesco Saturno, Alberto Nicolò Galvano, Mariachiara Ippolito, Leo Massari, Margherita Bianconi, Gaetano Caruso, Riccardo Ragazzi, Carlo Alberto Volta, Silvia Mongodi, Francesco Mojoli, Filippo Riccone, Sabino Scolletta, Sebastiano Macheda, Serafino Vulcano, Giovanni Cosco, Eugenio Vadalà, Erika Taddei, Alessandro Isirdi
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medicine.medical_specialty ,R895-920 ,Physical examination ,030204 cardiovascular system & hematology ,law.invention ,NO ,Medical physics. Medical radiology. Nuclear medicine ,03 medical and health sciences ,0302 clinical medicine ,Randomized controlled trial ,law ,medicine ,Radiology, Nuclear Medicine and imaging ,030212 general & internal medicine ,Prospective cohort study ,lung ultrasound ,Hip surgery ,Hip fracture ,Radiological and Ultrasound Technology ,medicine.diagnostic_test ,business.industry ,Interventional radiology ,medicine.disease ,Surgery ,Orthopedic surgery ,Observational study ,Original Article ,business - Abstract
Background Hip fracture is one of the most common orthopedic causes of hospital admission in frail elderly patients. Hip fracture fixation in this class of patients is considered a high-risk procedure. Preoperative physical examination, plasma natriuretic peptide levels (BNP, Pro-BNP), and cardiovascular scoring systems (ASA-PS, RCRI, NSQIP-MICA) have all been demonstrated to underestimate the risk of postoperative complications. We designed a prospective multicenter observational study to assess whether preoperative lung ultrasound examination can predict better postoperative events thanks to the additional information they provide in the form of “indirect” and “direct” cardiac and pulmonary lung ultrasound signs. Methods LUSHIP is an Italian multicenter prospective observational study. Patients will be recruited on a nation-wide scale in the 12 participating centers. Patients aged > 65 years undergoing spinal anesthesia for hip fracture fixation will be enrolled. A lung ultrasound score (LUS) will be generated based on the examination of six areas of each lung and ascribing to each area one of the four recognized aeration patterns—each of which is assigned a subscore of 0, 1, 2, or 3. Thus, the total score will have the potential to range from a minimum of 0 to a maximum of 36. The association between 30-day postoperative complications of cardiac and/or pulmonary origin and the overall mortality will be studied. Considering the fact that cardiac complications in patients undergoing hip surgery occur in approx. 30% of cases, to achieve 80% statistical power, we will need a sample size of 877 patients considering a relative risk of 1.5. Conclusions Lung ultrasound (LU), as a tool within the anesthesiologist’s armamentarium, is becoming increasingly widespread, and its use in the preoperative setting is also starting to become more common. Should the study demonstrate the ability of LU to predict postoperative cardiac and pulmonary complications in hip fracture patients, a randomized clinical trial will be designed with the scope of improving patient outcome. Trial registration ClinicalTrials.gov, NCT04074876. Registered on August 30, 2019.
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- 2021
3. Lung ultrasound signs and cytokine profile in Covid-19 patients: a case series
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Delrio, S, Vetrugno, L, Orso, D, Deana, C, D'Andrea, N, and Bove, T
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- 2020
4. Lung ultrasound signs and cytokine profile in Covid-19 patients: a case series.
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Delrio, S., Vetrugno, L., Orso, D., Deana, C., D'Andrea, N., and Bove, T.
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- 2020
5. TU-C-220-07: Photoacoustic and Ultrasound Imaging Using a Hemispherical Aperture
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Kruger, R, primary, Lam, R, additional, Reinecke, D, additional, and DelRio, S, additional
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- 2011
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6. A Comparison of Four Stimulation Patterns in Axillary Block
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RODRIGUEZ, J, primary, TABOADA, M, additional, DELRIO, S, additional, BARCENA, M, additional, and ALVAREZ, J, additional
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- 2005
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7. 336 Carboplatin and etoposide in patients (pts) with non small cell lung cancer. A phase II study
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Brugia, M., primary, Delrio, S., additional, Tagliaventi, M., additional, Trippa, F., additional, Piermarini, V., additional, and Buzzi, F., additional
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- 1997
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8. Quantification of DNA Uptake by Dictyostelium discoideum Amoebae and the Stability of the DNA during Growth and Development
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SAMPSON, J., primary, DELRIO, S., additional, and TOWN, C. D., additional
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- 1985
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9. Organ donation after extracorporeal cardiopulmonary resuscitation for refractory out-of-hospital cardiac arrest in a metropolitan cardiac arrest centre in Milan, Italy.
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Bonizzoni MA, Scquizzato T, Pieri M, Delrio S, Nardelli P, Ortalda A, Dell'Acqua A, and Scandroglio AM
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- Humans, Italy epidemiology, Male, Female, Middle Aged, Aged, Retrospective Studies, Tissue Donors statistics & numerical data, Adult, Out-of-Hospital Cardiac Arrest therapy, Out-of-Hospital Cardiac Arrest mortality, Tissue and Organ Procurement methods, Extracorporeal Membrane Oxygenation methods, Extracorporeal Membrane Oxygenation statistics & numerical data, Cardiopulmonary Resuscitation methods, Cardiopulmonary Resuscitation statistics & numerical data
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Introduction: Extracorporeal cardiopulmonary resuscitation (ECPR) may improve survival in refractory out-of-hospital cardiac arrest (OHCA) but also expand the donor pool as these patients often become eligible for organ donation. Our aim is to describe the impact of organ donation in OHCA patients treated with ECPR in a high-volume cardiac arrest centre., Methods: Rate of organ donation (primary outcome), organs harvested, a composite of patient survival with favourable neurological outcome or donation of ≥1 solid organ (ECPR benefit), and the potential total number of individuals benefiting from ECPR (survivors with favourable neurological outcome and potential recipients of one solid organ) were analysed among all-rhythms refractory OHCA patients treated with ECPR between January 2013-November 2022 at San Raffaele Hospital in Milan, Italy., Results: Among 307 adults with refractory OHCA treated with ECPR (95% witnessed, 66% shockable, low-flow 70 [IQR 58-81] minutes), 256 (83%) died during hospital stay, 33% from brain death. Donation of at least one solid organ occurred in 58 (19%) patients, 53 (17%) after determination of brain death and 5 (1.6%) after determination of circulatory death, contributing a total of 167 solid organs (3.0 [IQR 2.5-4.0] organs/donor). Overall, 196 individuals (29 survivors with favourable neurological outcome and 167 potential recipients of 1 solid organ) possibly benefited from ECPR. ECPR benefit composite outcome was achieved in 87 (28%) patients. Solid organ donation decreased from 19% to 16% in patients with low-flow <60 min and to 11% with low-flow <60 min and initial shockable rhythm., Conclusions: When ECPR fails in patients with refractory OHCA, organ donation after brain or circulatory death can help a significant number of patients awaiting transplantation, enhancing the overall benefit of ECPR. ECPR selection criteria may affect the number of potential organ donors., Competing Interests: Declaration of competing interest The authors declare the following financial interests/personal relationships which may be considered as potential competing interests: ‘TS is the Social Media Editor of Resuscitation’., (Copyright © 2024 Elsevier B.V. All rights reserved.)
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- 2024
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10. Temporary mechanical circulatory support with Impella in cardiac surgery: A systematic review.
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Pieri M, D'Andria Ursoleo J, Nardelli P, Ortalda A, Ajello S, Delrio S, Fominskiy E, and Scandroglio AM
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- Adult, Humans, Retrospective Studies, Stroke Volume, Ventricular Function, Left, Shock, Cardiogenic surgery, Shock, Cardiogenic etiology, Treatment Outcome, Cardiac Surgical Procedures adverse effects, Heart-Assist Devices adverse effects
- Abstract
Introduction: Perioperative cardiogenic shock (CS) in cardiac surgery is still burdened by a high mortality risk. The introduction of Impella pumps in the therapeutic armory of temporary mechanical circulatory support (tMCS) has potential implications to improve the management of complex cases, although it has never been systematically addressed. We performed a systematic review of the reported use of tMCS with Impella in cardiac surgery., Methods: We searched PubMed for all original studies on the Impella use in adult patients in cardiac surgery., Results: Nineteen studies (out of 151 identified by search string) were included. All studies were observational and all but one (95%) were retrospective. Seven studies focused on the implantation of Impella in the pre-operative setting (coronary or valvular surgery), either as a prophylactic device in high-risk cases (3 studies) or in patients with CS as stabilization tool prior to cardiac surgery procedure (4 studies). Three studies reported the use of Impella as periprocedural support for percutaneous valvular procedure, three as bridge to heart replacement, and six for postcardiotomy CS. Impella support had a low complication rate and was successful in supporting hemodynamics pre-, intra- and postoperatively. Most consistently reported data were left-ventricular ejection fraction at implant, short-term survival and weaning rate., Conclusions: tMCS with Impella in cardiac surgery patients is feasible and successful. It can be applied in selected cardiac surgery patients and presents advantages over other types of support. Systematic prospective studies are needed to standardize indications for implant and management of surgical issues, and to identify which patients may benefit., (Copyright © 2023 Elsevier B.V. All rights reserved.)
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- 2024
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11. Extracorporeal cardiopulmonary resuscitation for refractory out-of-hospital cardiac arrest: 10-year experience in a metropolitan cardiac arrest centre in Milan, Italy.
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Scquizzato T, Calabrò MG, Franco A, Fominskiy E, Pieri M, Nardelli P, Delrio S, Altizio S, Ortalda A, Melisurgo G, Ajello S, Landoni G, Zangrillo A, and Scandroglio AM
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Introduction: Growing evidence supports extracorporeal cardiopulmonary resuscitation (ECPR) for refractory out-of-hospital cardiac arrest (OHCA) patients, especially in experienced centres. We present characteristics, treatments, and outcomes of patients treated with ECPR in a high-volume cardiac arrest centre in the metropolitan area of Milan, Italy and determine prognostic factors., Methods: Refractory OHCA patients treated with ECPR between 2013 and 2022 at IRCCS San Raffaele Scientific Institute in Milan had survival and neurological outcome assessed at hospital discharge., Results: Out of 307 consecutive OHCA patients treated with ECPR (95% witnessed, 66% shockable, low-flow 70 [IQR 58-81] minutes), 17% survived and 9.4% had favourable neurological outcome. Survival and favourable neurological outcome increased to 51% (OR = 8.7; 95% CI, 4.3-18) and 28% (OR = 6.3; 95% CI, 2.8-14) when initial rhythm was shockable and low-flow (time between CPR initiation and ROSC or ECMO flow) ≤60 minutes and decreased to 9.5% and 6.3% when low-flow exceeded 60 minutes (72% of patients). At multivariable analysis, shockable rhythm (aOR for survival = 2.39; 95% CI, 1.04-5.48), shorter low-flow (aOR = 0.95; 95% CI, 0.94-0.97), intermittent ROSC (aOR = 2.5; 95% CI, 1.2-5.6), and signs of life (aOR = 3.7; 95% CI, 1.5-8.7) were associated with better outcomes. Survival reached 10% after treating 104 patients ( p for trend <0.001)., Conclusions: Patients with initial shockable rhythm, intermittent ROSC, signs of life, and low-flow ≤60 minutes had higher success of ECPR for refractory OHCA. Favourable outcomes were possible beyond 60 minutes of low-flow, especially with concomitant favourable prognostic factors. Outcomes improved as the case-volume increased, supporting treatment in high-volume cardiac arrest centres., Competing Interests: TS is the Social Media Editor of Resuscitation Plus. All other authors declare they have no financial interests/personal relationships which may be considered as potential competing interests., (© 2023 The Authors.)
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- 2023
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12. Can Lung Ultrasound Be the Ideal Monitoring Tool to Predict the Clinical Outcome of Mechanically Ventilated COVID-19 Patients? An Observational Study.
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Vetrugno L, Meroi F, Orso D, D'Andrea N, Marin M, Cammarota G, Mattuzzi L, Delrio S, Furlan D, Foschiani J, Valent F, and Bove T
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Background: During the COVID-19 pandemic, lung ultrasound (LUS) has been widely used since it can be performed at the patient's bedside, does not produce ionizing radiation, and is sufficiently accurate. The LUS score allows for quantifying lung involvement; however, its clinical prognostic role is still controversial., Methods: A retrospective observational study on 103 COVID-19 patients with respiratory failure that were assessed with an LUS score at intensive care unit (ICU) admission and discharge in a tertiary university COVID-19 referral center., Results: The deceased patients had a higher LUS score at admission than the survivors (25.7 vs. 23.5; p -value = 0.02; cut-off value of 25; Odds Ratio (OR) 1.1; Interquartile Range (IQR) 1.0-1.2). The predictive regression model shows that the value of LUSt0 (OR 1.1; IQR 1.0-1.3), age (OR 1.1; IQR 1.0-1.2), sex (OR 0.7; IQR 0.2-3.6), and days in spontaneous breathing (OR 0.2; IQR 0.1-0.5) predict the risk of death for COVID-19 patients (Area under the Curve (AUC) 0.92). Furthermore, the surviving patients showed a significantly lower difference between LUS scores at admission and discharge (mean difference of 1.75, p -value = 0.03)., Conclusion: Upon entry into the ICU, the LUS score may play a prognostic role in COVID-19 patients with ARDS. Furthermore, employing the LUS score as a monitoring tool allows for evaluating the patients with a higher probability of survival.
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- 2022
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13. Phlegmasia cerulea dolens superimposed on disseminated intravascular coagulation in COVID-19.
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Orso D, Mattuzzi L, Scapol S, Delrio S, Vetrugno L, and Bove T
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- Adult, Gangrene, Humans, Middle Aged, SARS-CoV-2, COVID-19, Disseminated Intravascular Coagulation complications, Thrombophlebitis etiology, Venous Thrombosis etiology
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COVID-19 infection has several cardiovascular implications, and coagulopathy is a common abnormality in these patients, often coupled with elevated plasma fibrinogen and D-dimer levels, contributing to adverse outcomes. Phlegmasia cerulea dolens (PCD) is a rare manifestation of deep vein thrombosis. It is life-threatening and can rapidly lead to venous gangrene of the extremity. Only a few cases of COVID-19 associated with PCD are reported in the literature, despite thromboembolism being the common paradigm between the two diseases. We present the case of a 64-year-old adult with acute severe COVID-19 pneumonia who developed PCD despite constantly elevated activated partial thromboplastin time and international normalized ratio.
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- 2021
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14. Vaginal delivery in a patient with severe aortic stenosis under epidural analgesia, a case report.
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Driul L, Meroi F, Sala A, Delrio S, Pavoni D, Barbariol F, Londero A, Dogareschi T, Spasiano A, Vetrugno L, and Bove T
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- Adult, Echocardiography methods, Electrocardiography, Female, Humans, Infant, Newborn, Pregnancy, Severity of Illness Index, Analgesia, Epidural methods, Aortic Valve Stenosis diagnosis, Delivery, Obstetric methods, Pregnancy Complications, Cardiovascular
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Background: A history of previous cardiac disease increases the maternal mortality risk by as much as 100%. There is no consensus on the absolute contraindications to vaginal delivery in valvular heart disease, but central regional anesthesia is traditionally considered contraindicated in patients with severe aortic stenosis., Case Presentation: A 29-year-old primigravid woman with severe aortic stenosis was admitted to the obstetrics department for programmed labor induction. With epidural anesthesia and mini-invasive hemodynamic monitoring labor and operative vaginal delivery were well tolerated, and hemodynamic stability was always maintained., Conclusions: Epidural analgesia and oxytocin induction are possible for the labor management of parturients with severe aortic stenosis given that continuous non-invasive followed by invasive hemodynamic monitoring can be provided and given the absence of any obstetric or cardiologic contraindications and the strong will of the patient.
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- 2020
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15. Assessment of Pleural Effusion and Small Pleural Drain Insertion by Resident Doctors in an Intensive Care Unit: An Observational Study.
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Vetrugno L, Guadagnin GM, Barbariol F, D'Incà S, Delrio S, Orso D, Girometti R, Volpicelli G, and Bove T
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Small-bore pleural drainage device insertion has become a first-line therapy for the treatment of pleural effusions (PLEFF) in the intensive care unit; however, no data are available regarding the performance of resident doctors in the execution of this procedure. Our aim was to assess the prevalence of complications related to ultrasound-guided percutaneous small-bore pleural drain insertion by resident doctors. In this single-center observational study, the primary outcome was the occurrence of complications. Secondary outcomes studied were as follows: estimation of PLEFF size by ultrasound and postprocedure changes in PaO
2 /FiO2 ratio. In all, 87 pleural drains were inserted in 88 attempts. Of these, 16 were positioned by the senior intensivist following a failed attempt by the resident, giving a total of 71 successful placements performed by residents. In 13 cases (14.8%), difficulties were encountered in advancing the catheter over the guidewire. In 16 cases (18.4%), the drain was positioned by a senior intensivist after a failed attempt by a resident. In 8 cases (9.2%), the final chest X-ray revealed a kink in the catheter. A pneumothorax was identified in 21.8% of cases with a mean size (±SD) of just 10 mm (±6; maximum size: 20 mm). The mean size of PLEFF was 57.4 mm (±19.9), corresponding to 1148 mL (±430) according to Balik's formula. Ultrasound-guided placement of a small-bore pleural drain by resident doctors is a safe procedure, although it is associated with a rather high incidence of irrelevant pneumothoraces., Competing Interests: Declaration of Conflicting Interests:The author(s) declared no potential conflicts of interest with respect to the research, authorship, and/or publication of this article.- Published
- 2019
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16. Chronic treatment with the mitochondrial peptide humanin prevents age-related myocardial fibrosis in mice.
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Qin Q, Mehta H, Yen K, Navarrete G, Brandhorst S, Wan J, Delrio S, Zhang X, Lerman LO, Cohen P, and Lerman A
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- Age Factors, Aldehydes metabolism, Animals, Apoptosis drug effects, Cardiomyopathies metabolism, Cardiomyopathies pathology, Cell Line, Tumor, Cell Proliferation drug effects, Cytokines metabolism, Cytoprotection, Female, Fibroblasts metabolism, Fibroblasts pathology, Fibrosis, Glycogen Synthase Kinase 3 beta metabolism, Humans, Matrix Metalloproteinase 2 metabolism, Mice, Inbred C57BL, Myocytes, Cardiac metabolism, Myocytes, Cardiac pathology, Proto-Oncogene Proteins c-akt metabolism, Receptor, Fibroblast Growth Factor, Type 2 metabolism, Signal Transduction drug effects, Transforming Growth Factor beta1 metabolism, Aging metabolism, Aging pathology, Cardiomyopathies prevention & control, Fibroblasts drug effects, Intracellular Signaling Peptides and Proteins pharmacology, Myocytes, Cardiac drug effects, Protective Agents pharmacology
- Abstract
Cardiac fibrosis is a biological process that increases with age and contributes to myocardial dysfunction. Humanin (HN) is an endogenous mitochondria-derived peptide that has cytoprotective effects and reduces oxidative stress. The present study aimed to test the hypothesis that chronic supplementation of exogenous HN in middle-aged mice could prevent and reverse cardiac fibrosis and apoptosis in the aging heart. Female C57BL/6N mice at 18 mo of age received 14-mo intraperitoneal injections of vehicle (old group; n = 6) or HN analog (HNG; 4 mg/kg 2 times/wk, old + HNG group, n = 8) and were euthanized at 32 mo of age. C57BL/6N female mice (young group, n = 5) at 5 mo of age were used as young controls. HNG treatment significantly increased the ratio of cardiomyocytes to fibroblasts in aging hearts, as shown by the percentage of each cell type in randomly chosen fields after immunofluorescence staining. Furthermore, the increased collagen deposition in aged hearts was significantly reduced after HNG treatment, as indicated by picrosirius red staining. HNG treatment also reduced in aging mice cardiac fibroblast proliferation (5'-bromo-2-deoxyuridine staining) and attenuated transforming growth factor-β
1 , fibroblast growth factor-2, and matrix metalloproteinase-2 expression (immunohistochemistry or real-time PCR). Myocardial apoptosis was inhibited in HNG-treated aged mice (TUNEL staining). To decipher the pathway involved in the attenuation of the myocardial fibrosis by HNG, Western blot analysis was done and showed that HNG upregulated the Akt/glycogen synthase kinase -3β pathway in aged mice. Exogenous HNG treatment attenuated myocardial fibrosis and apoptosis in aged mice. The results of the present study suggest a role for the mitochondria-derived peptide HN in the cardioprotection associated with aging. NEW & NOTEWORTHY Cardiac fibrosis is a biological process that increases with age and contributes to myocardial dysfunction. Humanin is an endogenous mitochondria-derived peptide that has cytoprotective effects and reduces oxidative stress. Here, we demonstrate, for the first time, that exogenous humanin treatment attenuated myocardial fibrosis and apoptosis in aging mice. We also detected upregulated Akt/glycogen synthase kinase-3β pathway in humanin analog-treated mice, which might be the mechanism involved in the cardioprotective effect of humanin analog in aging mice.- Published
- 2018
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17. Downregulation of circulating MOTS-c levels in patients with coronary endothelial dysfunction.
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Qin Q, Delrio S, Wan J, Jay Widmer R, Cohen P, Lerman LO, and Lerman A
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- Adult, Animals, Aorta, Thoracic drug effects, Aorta, Thoracic metabolism, Biomarkers blood, Coronary Artery Disease diagnostic imaging, Coronary Circulation drug effects, Coronary Vessels drug effects, Coronary Vessels physiopathology, Down-Regulation drug effects, Endothelium, Vascular drug effects, Endothelium, Vascular physiopathology, Female, Humans, Male, Mice, Mice, Inbred C57BL, Middle Aged, Mitochondrial Proteins pharmacology, Organ Culture Techniques, Rats, Rats, Wistar, Coronary Artery Disease blood, Coronary Circulation physiology, Coronary Vessels metabolism, Down-Regulation physiology, Endothelium, Vascular metabolism, Mitochondrial Proteins blood
- Abstract
Background: MOTS-c is one of the newly identified mitochondrial-derived peptides which play a role in regulating metabolic homeostasis. The current study aimed to investigate whether circulating MOTS-c levels are also associated with endothelial dysfunction(ED) in patients without significant structural coronary lesions., Methods: Forty patients undergoing coronary angiography and endothelial function testing for clinical indications of recurrent angina with no structural coronary lesions were included in the study. They were divided into two groups based on coronary blood flow response to intracoronary acetylcholine (ACh) as normal endothelial function (≥ 50% increase from baseline) or ED, (n=20 each). Aortic plasma samples were collected at the time of catheterization for analysis of circulating MOTS-c levels by ELISA. The effect of MOTS-c on vascular reactivity was assessed in organ chambers using aortic rings collected from rats and renal artery stenosis (RAS) mice., Results: Baseline characteristics were similar between the two groups. MOTS-c plasma levels were lower in patients with ED compared with patients with normal endothelial function (p=0.007). Furthermore, plasma MOTS-c levels were positively correlated with microvascular (p=0.01) and epicardial (p=0.02) coronary endothelial function. Although MOTS-c did not have direct vasoactive effects, pretreating aortic rings from rats or RAS mice with MOTS-c (2μg/ml) improved vessel responsiveness to ACh compared with vessels without MOTS-c treatment., Conclusion: Lower circulating endogenous MOTS-c levels in human subjects are associated with impaired coronary endothelial function. In rodents, MOTS-c improves endothelial function in vitro. Thus, MOTS-c represents a novel potential therapeutic target in patients with ED., (Copyright © 2017 Elsevier Ireland Ltd. All rights reserved.)
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- 2018
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18. Quantification of DNA uptake by Dictyostelium discoideum amoebae and the stability of the DNA during growth and development.
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Sampson J, Delrio S, and Town CD
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- Dictyostelium genetics, Plasmids, Transformation, Genetic, DNA, Fungal metabolism, Dictyostelium growth & development
- Abstract
We have developed a simple and accurate method to determine the amount of intact plasmid DNA taken up and retained by Dictyostelium discoideum amoebae during various transformation protocols. We have used this method to compare the efficiency of three different methods for introducing foreign DNA into D. discoideum amoebae. Both a calcium phosphate and a spheroplast fusion procedure gave good uptake, but no intracellular plasmid DNA was detectable after calcium chloride treatment. The exogenous DNA was rapidly lost after transformation but was 20-fold more stable during starvation rather than growth conditions, suggesting a possible approach to improving transformation efficiency. No transient expression of neomycin phosphotransferase activity of any of the heterologous animal or plant promoters used could be detected using a sensitive gel assay procedure.
- Published
- 1985
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