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9 results on '"Delzor, Aurélie"'

2. Lentiviral Vectors: A Powerful Tool to Target Astrocytes In Vivo

Author

Aurélie, Delzor, Delzor, Aurélie, Carole, Escartin, Escartin, Carole, Nicole, Déglon, Déglon, Nicole, Laboratoire des Maladies Neurodégénératives - UMR 9199 (LMN), Service MIRCEN (MIRCEN), Institut de Biologie François JACOB (JACOB), Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay-Institut de Biologie François JACOB (JACOB), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS), Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS)-Institut de Biologie François JACOB (JACOB), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS)-Institut de Biologie François JACOB (JACOB), and Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Centre National de la Recherche Scientifique (CNRS)

Subjects
[SDV.NEU.NB]Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC]/Neurobiology, Transgene, Genetic Vectors, Clinical Biochemistry, Biology, Viral vector, 03 medical and health sciences, Transduction (genetics), 0302 clinical medicine, Central Nervous System Diseases, Transduction, Genetic, Drug Discovery, Gene expression, Animals, Humans, Transgenes, ComputingMilieux_MISCELLANEOUS, Tropism, 030304 developmental biology, Neurons, Pharmacology, Regulation of gene expression, 0303 health sciences, Lentivirus, Virus Internalization, Molecular biology, Cell biology, Transgenesis, MicroRNAs, Viral Tropism, Gene Expression Regulation, Astrocytes, Gene Targeting, Tissue tropism, Molecular Medicine, 030217 neurology & neurosurgery
Abstract

The morphological and functional diversity of astrocytes, and their essential contribution in physiological and pathological conditions, are starting to emerge. However, experimental systems to investigate neuron-glia interactions and develop innovative approaches for the treatment of central nervous system (CNS) disorders are still very limited. Fluorescent reporter genes have been used to visualize populations of astrocytes and produce an atlas of gene expression in the brain. Knock-down or knock-out of astrocytic proteins using transgenesis have also been developed, but these techniques remain complex and time-consuming. Viral vectors have been developed to overexpress or silence genes of interest as they can be used for both in vitro and in vivo studies in adult mammalian species. In most cases, high transduction efficiency and long-term transgene expression are observed in neurons but there is limited expression in astrocytes. Several strategies have been developed to shift the tropism of lentiviral vectors (LV) and allow local and controlled gene expression in glial cells. In this review, we describe how modifications of the interaction between the LV envelope glycoprotein and the surface receptor molecules on target cells, or the integration of cell-specific promoters and miRNA post-transcriptional regulatory elements have been used to selectively express transgenes in astrocytes.

Published
2013

3. Incidence de la Sclérose Latérale Amyotrophique (SLA) en Limousin (2000-2011) : résultats du premier registre français des cas de SLA (Registre Fralim)

Author

Hamidou, Bello, Couratier, Philippe, Nicol, Marie, Delzor, Aurélie, Raymondeau, Marie, Druet-Cabanac, Michel, Lautrette, Géraldine, Boumédiène, Farid, Preux, Pierre-Marie, Marin, Benoît, Neuroépidémiologie Tropicale (NET), Institut Génomique, Environnement, Immunité, Santé, Thérapeutique (GEIST), Université de Limoges (UNILIM)-Université de Limoges (UNILIM)-CHU Limoges-Institut d'Epidémiologie Neurologique et de Neurologie Tropicale-Institut National de la Santé et de la Recherche Médicale (INSERM), Service de Neurologie [CHU Limoges], CHU Limoges, Service de Santé au Travail [CHU Limoges], Laboratoire de Géographie Physique et Environnementale (GEOLAB), Université Blaise Pascal - Clermont-Ferrand 2 (UBP)-Université Clermont Auvergne [2017-2020] (UCA [2017-2020])-Université Clermont Auvergne (UCA)-Institut Sciences de l'Homme et de la Société (IR SHS UNILIM), Université de Limoges (UNILIM)-Université de Limoges (UNILIM)-Centre National de la Recherche Scientifique (CNRS), Service de l'Information Médicale et de l'Évaluation [CHU Limoges] (SIME), Laboratoire de Biostatistique et d'Informatique Médicale, Université de Limoges (UNILIM), CHU Limoges-Institut d'Epidémiologie Neurologique et de Neurologie Tropicale-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut Génomique, Environnement, Immunité, Santé, Thérapeutique (GEIST), Université de Limoges (UNILIM)-Université de Limoges (UNILIM), Université Blaise Pascal - Clermont-Ferrand 2 (UBP)-Institut Sciences de l'Homme et de la Société (IR SHS UNILIM), Université de Limoges (UNILIM)-Université de Limoges (UNILIM)-Université Clermont Auvergne [2017-2020] (UCA [2017-2020])-Centre National de la Recherche Scientifique (CNRS)-Université Clermont Auvergne (UCA), and Grelier, Elisabeth

Subjects
[SDV.SPEE] Life Sciences [q-bio]/Santé publique et épidémiologie, [SDV.SPEE]Life Sciences [q-bio]/Santé publique et épidémiologie, ComputingMilieux_MISCELLANEOUS
Abstract

International audience

Published
2014

4. Lentiviral Vectors in Huntington's Disease Research and Therapy

Author

Delzor Aurélie, Escartin Carole, and Déglon Nicole

Abstract

We describe here the potential of viral mediated gene transfer for the modeling and treatment of Huntington’s disease focusing in particular on strategies for the tissue specifi c targeting of various CNS cells. The protocols described here cover the design of lentiviral vectors strategies for modifying their tropism including the use of various envelopes and tissue specifi c promoters and the potential of miRNA to regulate transgene expression.

Published
2014

8. Searching for a link between the L-BMAA neurotoxin and amyotrophic lateral sclerosis: a study protocol of the French BMAALS programme.

Author

Delzor, Aurélie, Couratier, Philippe, Boumédiène, Farid, Nicol, Marie, Druet-Cabanac, Michel, Paraf, François, Méjean, Annick, Ploux, Olivier, Leleu, Jean-Philippe, Brient, Luc, Lengronne, Marion, Pichon, Valérie, Combès, Audrey, El Abdellaoui, Saïda, Bonneterre, Vincent, Lagrange, Emmeline, Besson, Gérard, Bicout, Dominique J., Boutonnat, Jean, and Camu, William

Abstract

Introduction: Amyotrophic lateral sclerosis (ALS) is the most common motor neurone disease. It occurs in two forms: (1) familial cases, for which several genes have been identified and (2) sporadic cases, for which various hypotheses have been formulated. Notably, the β-N-methylamino-L-alanine (L-BMAA) toxin has been postulated to be involved in the occurrence of sporadic ALS. The objective of the French BMAALS programme is to study the putative link between L-BMAA and ALS. Methods and analysis: The programme covers the period from 1 January 2003 to 31 December 2011. Using multiple sources of ascertainment, all the incident ALS cases diagnosed during this period in the area under study (10 counties spread over three French regions) were collected. First, the standardised incidence ratio will be calculated for each municipality under concern. Then, by applying spatial clustering techniques, overincidence and underincidence zones of ALS will be sought. A case-control study, in the subpopulation living in the identified areas, will gather information about patients’ occupations, leisure activities and lifestyle habits in order to assess potential risk factors to which they are or have been exposed. Specimens of drinking water, food and biological material (brain tissue) will be examined to assess the presence of L-BMAA in the environment and tissues of ALS cases and controls. Ethics and dissemination: The study has been reviewed and approved by the French ethical committee of the CPP SOOM IV (Comité de Protection des Personnes Sud-Ouest & Outre-Mer IV). The results will be published in peer-reviewed journals and presented at national and international conferences. [ABSTRACT FROM AUTHOR]

Published
2014
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9. Lentiviral vectors: a powerful tool to target astrocytes in vivo.

Author

Delzor A, Escartin C, and Déglon N

Subjects
Animals, Central Nervous System Diseases physiopathology, Central Nervous System Diseases therapy, Gene Expression Regulation, Humans, MicroRNAs genetics, MicroRNAs metabolism, Neurons physiology, Transgenes, Viral Tropism, Virus Internalization, Astrocytes physiology, Gene Targeting methods, Genetic Vectors, Lentivirus genetics, Lentivirus physiology, Transduction, Genetic
Abstract

The morphological and functional diversity of astrocytes, and their essential contribution in physiological and pathological conditions, are starting to emerge. However, experimental systems to investigate neuron-glia interactions and develop innovative approaches for the treatment of central nervous system (CNS) disorders are still very limited. Fluorescent reporter genes have been used to visualize populations of astrocytes and produce an atlas of gene expression in the brain. Knock-down or knock-out of astrocytic proteins using transgenesis have also been developed, but these techniques remain complex and time-consuming. Viral vectors have been developed to overexpress or silence genes of interest as they can be used for both in vitro and in vivo studies in adult mammalian species. In most cases, high transduction efficiency and long-term transgene expression are observed in neurons but there is limited expression in astrocytes. Several strategies have been developed to shift the tropism of lentiviral vectors (LV) and allow local and controlled gene expression in glial cells. In this review, we describe how modifications of the interaction between the LV envelope glycoprotein and the surface receptor molecules on target cells, or the integration of cell-specific promoters and miRNA post-transcriptional regulatory elements have been used to selectively express transgenes in astrocytes.

Published
2013
Full Text
View/download PDF

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