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Your search keyword '"Deng, Huiming"' showing total 19 results
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19 results on '"Deng, Huiming"'

18. Phase separation: a new window in RALF signaling.

Author

Zhang Z, Deng H, Hu S, and Han H

Abstract

Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.

Published
2024
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19. Assessment of Feng-Liao-Chang-Wei-Kang as a potential inducer of cytochrome P450 3A4 and pregnane X receptors.

Author

Xie Y, Li Y, Huang M, Wu Q, Liu Q, Zhang J, Deng H, Liu M, and Huang L

Subjects
Animals, Cell Line, Tumor, Female, Hep G2 Cells, Hepatocytes drug effects, Humans, Medicine, Chinese Traditional methods, Promoter Regions, Genetic drug effects, Promoter Regions, Genetic genetics, RNA, Messenger genetics, Rats, Rats, Sprague-Dawley, Receptors, Cytoplasmic and Nuclear genetics, Transcriptional Activation genetics, Transfection methods, Up-Regulation drug effects, Up-Regulation genetics, Cytochrome P-450 CYP3A genetics, Drugs, Chinese Herbal pharmacology, Pregnane X Receptor genetics, Transcriptional Activation drug effects
Abstract

Feng-Liao-Chang-Wei-Kang (FLCWK), a traditional Chinese patent medicine, consists primarily of Polygonum hydropiper and Daphniphyllum calycinum roots. As a complex containing several kinds of flavonoids, FLCWK has the potential to impact the drug metabolism enzyme P450 3A4 (CYP3A4) and nuclear receptors. The purpose of this research was to probe the effects of FLCWK on CYP3A1, the homolog of CYP3A4 in rats, and to confirm whether FLCWK interferes with PXR and CAR-mediated transactivation of CYP3A4. The effects of FLCWK on Cyp3a1 mRNA, catalytic activity levels, and protein expression in Sprague-Dawley (SD) rat liver tissues were examined using real-time PCR, western blotting, and high-performance liquid chromatography (HPLC) assays, respectively. The efficacy of PXR and CAR on CYP3A4 transcriptional activity were detected using luciferase reporter assays and further research of the impact of FLCWK on CYP3A4 gene expression mediated by the PXR pathway was examined by transient transfection of PXR siRNA. FLCWK significantly increased Cyp3a1 mRNA, CYP3A1 activity, and protein expression levels in SD rats. FLCWK highly induced CYP3A4 luciferase activity mediated by PXR in PXRCYP3A4 co-transfected cells. A siRNA-mediated drop-off in PXR expression greatly cut the effect of FLCWK on CYP3A4 mRNA expression in HepG2 cells. These findings show that FLCWK up-regulates CYP3A4 levels via the PXR pathway. This effect should be considered being applied in clinical use as FLCWK has the potential to interact with other drugs.

Published
2018

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