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26 results on '"Deng, Jing-ti"'

6. Silence of TRIB3 suppresses atherosclerosis and stabilizes plaques in diabetic [ApoE.sup.-/-]/LDL [Receptor.sup.-/-] mice

Author

Wang, Zhi-hao, Shang, Yuan-yuan, Zhang, Shun, Zhong, Ming, Wang, Xu-ping, Deng, Jing-ti, Pan, Jie, Zhang, Yun, and Zhang, Wei

Subjects
Diabetes -- Research -- Physiological aspects, Atherosclerosis -- Research -- Physiological aspects, Health
Abstract

Insulin resistance triggers the developments of diabetes mellitus and atherosclerosis. Tribbles homolog 3 (TRIB3) is involved in insulin resistance. We aimed to investigate whether TRIB3 is implicated in diabetic atherosclerosis. Sixty 3-week-old apohpoprotein E ([ApoE.sup.-/-])/LDR receptor ([LDLR.sup.-/-]) mice were randomly divided into chow and diabetes groups. Diabetes was induced by a high-fat and high-sugar diet combined with low-dose streptozotocin. Mice in both groups were randomly divided into vehicle and TRIB3-silencing groups. After transfection, all mice were killed to evaluate the effects of TRIB3 on atherosclerosis. Silence of TRIB3 markedly decreased insulin resistance (P = 0.039) and glucose (P = 0.019), regardless of diabetes. Ultrasonography-measured parameters were similar in both groups, with and without silence of TRIB3. However, silence of TRIB3 decreased the aortic atherosclerotic burden (P = 1 x [10.sup.-13]). Further study showed that in brachiocephalic lesions, fibrous cap thickness, cap-to-core ratio, collagen content, and the number of smooth muscle cells were significantly increased (P < 0.01 for all) by silence of TRIB3, whereas lipid and macrophage contents remained unaltered, with the vulnerability index significantly reduced. Moreover, the numbers of apoptotic cells and macrophages in brachiocephalic lesions were both significantly decreased (P < 0.01 for both). Macrophage migration was decreased (P = 4 x [10.sup.-4]) by knocking down TRIB3, whereas adhesion and phagocytosis were increased (P < 0.05 for both). Silence of TRIB3 would diminish atherosclerotic burden and increase the plaque stability in diabetic mice. Diabetes 61:463-473, 2012, The increased prevalence of type 2 diabetes mellitus (T2DM) has focused attention on the elevated incidence of diabetic atherosclerosis (1). Insulin resistance (IR) is assumed to trigger the developments of [...]

Published
2012
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16. Fluorescence Linked Enzyme Chemoproteomic Strategy for Discovery of a Potent and Selective DAPK1 and ZIPK Inhibitor

Author

Carlson, David A., primary, Franke, Aaron S., additional, Weitzel, Douglas H., additional, Speer, Brittany L., additional, Hughes, Philip F., additional, Hagerty, Laura, additional, Fortner, Christopher N., additional, Veal, James M., additional, Barta, Thomas E., additional, Zieba, Bartosz J., additional, Somlyo, Avril V., additional, Sutherland, Cindy, additional, Deng, Jing Ti, additional, Walsh, Michael P., additional, MacDonald, Justin A., additional, and Haystead, Timothy A. J., additional

Published
2013
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26. [Diagnostic value of serum high molecular weight alkaline phosphatase in early detection of cholestatic jaundice in neonates].

Author

Shang XH, Lu XM, Deng JT, and Hu XY

Subjects
Diagnosis, Differential, Female, Humans, Immunoenzyme Techniques methods, Infant, Infant, Newborn, Male, Sensitivity and Specificity, gamma-Glutamyltransferase blood, Alkaline Phosphatase blood, Jaundice, Obstructive diagnosis
Abstract

Objective: To investigate the difference of serum high molecular weight alkaline phosphatase (HMAP) levels between biliary atresia (BA) and neonatal hepatitis (NH), and to develop a new differential method and early diagnostic indicators for cholestatic jaundice in neonates., Methods: Totally 31 patients with cholestatic jaundice seen between Aug. 2000 and Feb. 2002, including 15 cases with BA, 16 cases with NH, 30 healthy infants and 30 infants with non-cholestatic jaundice were enrolled in this study. Serum samples were obtained from each subject by using venipuncture. The samples were stored at -80 degrees C and analyzed within 6 months. A murine hybridoma producing monoclonal antibody to human high molecular weight alkaline phosphatase (MoAb HMAP-1) was prepared by using partially purified HMAP from human serum as the immunogen. The antibody did not cross-react with other alkaline phosphatase (ALP) isozymes. A monoclonal antibody immunocatalytic assay for HMAP in serum was developed by using MoAb HMAP-1 bound to nitrocellulose membrane discs. The serum total ALP (TALP) and gamma-GT were determined in the meantime, the hepatobiliary ultrasonography and scintigraphy were performed too. The data were analyzed with t test, chi-square test and percentage. Comparisons were made between BA and NH with their sensitivity and specificity in different methods., Results: Serum HMAP was detected in 14 of 15 patients of BA, in 2 of 16 NH patients, while in none of the healthy control group. The positive ratios of serum HMAP in BA and NH were 93.3% and 12.5%, respectively (P < 0.005). The sensitivity and specificity of serum HMAP in BA and NH were 93.3% and 87.5%, respectively. The sensitivity and specificity of TALP, gamma-GT and hepatobiliary scintigraphy were 80.0%, 73.3%, 86.7% and 62.5%, 68.8%, 62.5%, respectively, which were clearly lower than those of serum HMAP., Conclusions: The determination of serum HMAP was more sensitive and specific than the other methods tested. Therefore the method can be used as a useful indicator for cholestatic jaundice in neonates, although it needs further study.

Published
2003

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