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This analysis shows good intentions in the selection of valid and precise oxygen uptake ( M˙ O2 ) measurements by retaining only slopes of declining dissolved oxygen level in a respirometer that have very high values of the coefficient of determination, r2 , are not always successful at excluding nonlinear slopes. Much worse, by potentially removing linear slopes that have low r2 only because of a low signal-to-noise ratio, this procedure can overestimate the calculation of standard metabolic rate (SMR) of the fish. To remedy this possibility, a few simple diagnostic tools are demonstrated to assess the appropriateness of a given minimum acceptable r2 , such as calculating the proportion of rejected M˙ O2 determinations, producing a histogram of the r2 values and a plot of r2 as a function of M˙ O2 . The authors offer solutions for cases when many linear slopes have low r2 . The least satisfactory but easiest to implement is lowering the minimum acceptable r2 . More satisfactory solutions involve processing (smoothing) the raw signal of dissolved oxygen as a function of time to improve the signal-to-noise ratio and the r2 s.

In order to define the relative vulnerability of northern shrimp ( Pandalus borealis ) populations to the ongoing global warming, we compared the thermal performance curves for survival and growth in the first three pelagic larval stages from three populations of the Northwest Atlantic. Egg carrying females were obtained from different regions characterized by distinct sea surface temperature (SST) conditions for larval development in spring. Two independent experiments were conducted in two different years. In spring 2012, larvae from females captured in the Lower St Lawrence Estuary (LE) and in the Northeast Gulf of St Lawrence (GSL) were compared. In spring 2014, larvae from females captured in the LE and on the Labrador–Newfoundland Shelf (Northwest Atlantic, NWA) were used. The LE larvae were used both years and served as the reference population for comparisons. In 2012 and 2014, groups of 25 newly hatched northern shrimp larvae from each source population were incubated at six temperatures (0, 3, 6, 9, 12, and 15 °C) to monitor and compare survival and growth at moult. Northern shrimp larvae from the LE (warmer May–June SST) had a higher optimal temperature range for survival compared to larvae from the GSL and the NWA (colder May–June SST) populations. However, in 2012 growth performance at moult was reduced at higher temperatures for the LE population compared to the GSL population. The differences in thermal performance curves observed may suggest the presence of a certain level of local adaptation in response to the different regional SST regimes in spring–early summer. Northern shrimp larvae in the Northeast Gulf of St Lawrence and Northwest Atlantic shelf could benefit from warmer early-spring temperatures; however, larvae from the Lower Estuary may be closer to their upper tolerance limits and thus more likely at risk of negative impact of future warming of surface water masses.

WOS:000411848700011; We tested the hypothesis that the chronic residual effects of an acute exposure of European sea bass C Dicentrarchus labrax') to chemically dispersed crude oil is manifest in indices of hypoxic performance rather than aerobic performance.Sea bass were pre-screened with a hypoxia challenge test to establish their incipient lethal oxygen saturation (ILOS),but on discovering a wide breadth for individual ILOS values (2.6-11.0% O-2 saturation), fish were subsequently subdivided into either hypoxia sensitive (HS) or hypoxia tolerant (HT) phenotypes,traits that were shown to be experimentally repeatable.The HT phenotype had a lower ILOS and critical oxygen saturation (O-2crit) compared with the HS phenotype and switched to glycolytic metabolism at a lower dissolved oxygen, even though both phenotypes accumulated lactate and glucose to the same plasma concentrations at ILOS. As initially hypothesized, and regardless of the phenotype considered, we found no residual effect of oil on any of the indices of aerobic performance. Contrary to our hypothesis, however, oil exposure had no residual effect on any of the indices of hypoxic performance in the HS phenotype. In the HT phenotype, on the other hand, oil exposure had residual effects as illustrated by the impaired repeatability of hypoxia tolerance and also by the 24% increase in O-2crit, the 40% increase in scope for oxygen deficit, the 17% increase in factorial scope for oxygen deficit and the 57% increase in accumulated oxygen deficit. Thus, sea bass with a HT phenotype remained chronically impaired for a minimum of 167 days following an acute 24-h oil exposure while the HS phenotypes did not. We reasoned that impaired oxygen extraction at gill due to oil exposure activates glycolytic metabolism at a higher dissolved oxygen, conferring on the HT phenotype an inferior hypoxia resistance that might eventually compromise their ability to survive hypoxic episodes.

WOS:000569774900004; Coastal nursery areas are subjected to a wide range of natural and anthropogenic stressors, including global warming, which indirectly influence trophic food webs. A global rarefaction of n-3 polyunsaturated fatty acids (PUFA) in trophic networks is in progress. The aim of this study was to assess the effect of a reduction in the dietary availability of n-3 PUFA on some molecular and biochemical parameters related to lipid metabolism and oxidative stress response in juvenile European sea bass (Dicentrarchus labrax) raised at two temperatures (15 degrees C and 20 degrees C). Fish were fed for five months with a reference diet (RD; 1.65% n-3 PUFA on a dry matter basis, DM), used as a proxy of trophic networks where n-3 PUFA is plentiful, and a lower n-3 PUFA diet (LD; 0.73% n-3 PUFA DM), designed to mimic a decrease in n-3 PUFA resulting from global changes (the n-3 PUFA levels tested remained above the nutritional minimum required for this species). Results showed that diet did not affect the hepatic expression of some mRNA coding for transcriptional factors involved in regulating the metabolic pathways related to fatty acid bioconversion. Although our molecular analysis was limited to transcript expression, these data suggest the presence of a threshold in the nutritional supply of PUFA above which the activation of these molecular pathways does not occur. However, the expression for most of the transcripts tested was up-regulated at 20 degrees C. Despite the high peroxidation index in fish fed RD, very few modifications of the oxidative stress response were associated with diet. At 20 degrees C, an increase of the enzymatic antioxidant response was observed, but there was no correlation with the peroxidation index or malondialdehyde products.

The accumulation of CO2 in the atmosphere and resulting ocean acidification represent a threat to marine ecosystems. While acid–base regulatory capacity is well developed in marine fish, allowing compensation of extra-cellular pH during short-term hypercapnia, the possible energetic costs of such regulation during long-term exposure remain to be established. In this study, juvenile European sea bass (Dicentrarchus labrax) were exposed from 2 days post-hatching to three different ocean acidification scenarios: control (present condition, $$ P_{{{\text{CO}}_{2} }} $$ = 520 µatm, pH 7.9), moderate acidification ( $$ P_{{{\text{CO}}_{2} }} $$ = 950 µatm, pH 7.7), and high acidification ( $$ P_{{{\text{CO}}_{2} }} $$ = 1490 µatm, pH 7.5). After 1.5 years of exposure, fish aerobic metabolic capacities, as well as elements of their oxygen extraction and transport chain, were measured. Compared to control, $$ P_{{{\text{CO}}_{2} }} $$ treatments did not affect fish standard metabolic rate (SMR). However, the most severe acidification condition was associated with a significantly elevated maximum metabolic rate (MMR).This was supported by heavier gill system and higher blood haemoglobin concentration. A reduction of maximum cardiac frequency (fHmax) during incremental warming of anaesthetized fish was also observed in both acidification scenarios. On the other hand, the critical oxygen level (O2crit), the minimum oxygen level required to sustain SMR, did not differ among groups. The increased MMR, associated with maintained SMR, suggests that acid–base compensatory processes, although not increasing maintenance costs, may affect components of bass homeostasis, resulting in new internal physico-chemical conditions. The possibility that these alterations influence metabolic pathways and physiological functions involved in fish aptitude to maximally transport oxygen is discussed.

Depuis plusieurs annees, le concept d’approche ecosystemique est avance mondialement comme une solution possible aux problematiques de gestion liees a la deterioration des ecosystemes marins et de ...

In the Estuary and Gulf of St. Lawrence, northern shrimp (Pandalus borealis) and Greenland halibut (Reinhardtius hippoglossoides) are usually found at depths > 150 m and thus frequently inhabit hypoxic areas (18–50% saturation). The impact of a one-week exposure to different levels of dissolved oxygen (100, 40, 30, and 20% saturation) at 5 °C was evaluated in adult shrimp and juvenile Greenland halibut; the effect of acute exposure to severe hypoxia was also assessed in Greenland halibut. The activities of key enzymes involved in aerobic (citrate synthase [CS], cytochrome c oxidase [COX]) and anaerobic (pyruvate kinase [PK], phosphoenolpyruvate carboxykinase [PEPCK], lactate dehydrogenase [LDH]) pathways, and of enzymes involved in antioxidant defence (superoxide dismutase, glutathione peroxidase [GPx], and catalase [CAT]) were measured. Quantitative real-time PCR analysis was also performed in Greenland halibut. In northern shrimp exposed to chronic hypoxia, muscle CS activity decreased by ~ 40%. Muscle LDH activity was significantly reduced, with a more intense reduction in males. At the same time, hepatopancreas GPx activity increased under hypoxia, and this response was stronger in males. Overall, the results suggest the presence of a threshold above 40% saturation and higher hypoxia tolerance in males. In juvenile Greenland halibut, exposure to chronic hypoxia elicited a more wide-ranging enzymatic response than did acute exposure to severe hypoxia. Under chronic hypoxia, CS activity decreased and PK and LDH activity were respectively 46% and 57% lower than in normoxia. There were no major changes in the activity of antioxidant enzymes, but activity in normoxia was high compared to other fish species. Interestingly, the relative expression of genes coding for muscle COX (severe hypoxia), liver PEPCK (chronic), and CAT (chronic) activities were triggered in hypoxia. The absence of a corresponding change in enzyme activity makes the interpretation of these results difficult, but clearly there was a response at the transcription level. Overall, the results indicate that these two species are particularly well adapted to withstand severe hypoxia. -- Keywords : Aerobic pathwa ; Anaerobic pathway ; Antioxidant defence ; Metabolic capacity ; Gene expression ; Enzyme activity.

A mechanistic, prey surface-dependent model was expanded to describe the course and rate of gastric evacuation in predatory fishes feeding on crustacean prey with robust exoskeletons. This was accomplished by adding a layer of higher resistance to the digestive processes outside the inner softer parts of a prey cylinder abstraction and splitting up the prey evacuation into two stages: an initial stage where the exoskeleton is cracked and a second where the prey remains are digested and evacuated. The model was parameterized for crustaceans with different levels of armour fed to Atlantic cod Gadus morhua or whiting Merlangius merlangus and recovered from the stomachs at different post-prandial times. The prey species were krill Meganyctiphanes norvegica; shrimps and prawns Crangon crangon, Pandalus borealis, Pandalus montagui and Eualus macilentus; crabs Liocarcinus depurator and Chionoecetes opilio. In accordance with the apparent intraspecific isometric relationship between exoskeleton mass and total body mass, the model described stage duration and rate of evacuation of the crustacean prey independently of meal and prey sizes. The duration of the first stage increased (0-33 h) and the evacuation rate of both stages decreased (by a half) with increasing level of the crustacean armament in terms of chitin and ash. A common, interspecific parameterization of the model within each of the categories krill, shrimp and crab can probably be used if the contents of chitin and ash are similar among prey species per prey category. The model offers a simple way for estimating evacuation rates from stomach content data in order to obtain food consumption rates of wild fishes, provided that information about digestion stage of crustacean prey is available.

International audience; Like many marine species around the globe, several stocks of Atlantic cod (Gadus morhua) live in increasingly hypoxic waters. In the Gulf of Saint Lawrence (GSL) in Canada, the deep channels traversing the semi-enclosed sea exhibit year-round hypoxia, identified as one of the limiting factor for the recovery of GSL cod in its northern part. While many individuals in the northern GSL are known to venture in deeper, warmer, and more hypoxic waters of the Gulf channels, those in the southern GSL live in a shallower, colder, and more oxygenated environment. In this study, we use the modeling framework of the Dynamic Energy Budget (DEB) theory to disentangle the effects of hypoxia, temperature and food on the life-history traits of these two populations of cod in the GSL. Following recent advances by Thomas et al. (2018, this issue) on the mechanisms for the effects of hypoxia within the context of DEB theory, we implemented a correction of ingestion depending on dissolved oxygen (DO) saturation. We successfully developed and validated a set of parameters for a GSL Atlantic cod DEB model. Using simulations of historical growth trajectories from 1990 until 2004 estimated from data collected through fisheries research surveys, we found that temperature explained about half (48%) of the difference in length and 59% of the difference in mass between the two populations. The remaining proportion was attributed to exposure to hypoxia and food input. We also used our model to explore scenarios of duration, frequency, and intensity of hypoxia on cod's life-history traits, which showed that decreasing DO linearly reduces growth and reproduction while young cod seem to avoid impairing conditions resulting in limiting effects on developmental stages.

International audience; Hypoxia is a decrease in dissolved oxygen that causes physiological disturbances in marine fishes and invertebrates, including reduced mobility, growth rate and reproductive success, altered phenology and increased vulnerability to diseases. Under pressure from global changes such as warming or eutrophication, ocean and coastal ecosystems worldwide are becoming less oxygenated. In order to better understand the consequences of hypoxia on marine systems, there is a need for predicting hypoxia-induced changes from individual organisms to populations. By combining the effect of several stressors on species metabolic performances and life-history traits across their full life-cycle, the Dynamic Energy Budget (DEB) theory offers a suitable framework for studying the consequences of hypoxia on marine organisms and to envision an upscaling of these effects at population level through individual-based modelling approaches. Here, we propose a set of stylized biological facts which give a broad generalization of the effects of hypoxia on metabolic functions based on empirical findings. We used these stylized facts to derive assumptions on how to incorporate the effects of hypoxia on marine organisms in the framework of the DEB theory. We then validate some of these assumptions on marine species for which experimental datasets under hypoxic conditions and DEB parameters were available. We discuss the main issues that need to be dealt with, such as the various time-scales, ontogenic responses and multi-stressor effects which appear now as important targets of investigation for studying the effect of hypoxia on marine organisms.

Specific dynamic action (SDA) is the postprandial increase in oxygen uptake. Whereas it is easy to measure in fishes that remain calm and motionless during the entire digestion period, spontaneous locomotor activity is a frequent problem that leads to overestimation of SDA amplitude and magnitude (area under the curve, bound by the standard metabolic rate, SMR). Few studies have attempted to remove the effect of fish activity on SDA. A new method, non-parametric quantile regression, is described to estimate SDA even when pronounced circadian activity cycles are present. Data from juvenile Atlantic cod Gadus morhua are used to demonstrate its use and advantages compared with traditional techniques. Software (scripts in the R language) is provided to facilitate its use.

The problem of understanding the effect of the environment on fish activities and performance, in any generalized way, remains intractable. Solving this issue is, however, a key to addressing contemporary environmental concerns. As suggested 20 years ago by W. H. Neill, the authors returned to the drawing board, using as a background the conceptual scheme initially proposed by F. E. J. Fry. They revisited the effect of ambient oxygen availability upon fish metabolism and clarified the definitions of limiting, critical and incipient lethal oxygen (ILO) levels. The concepts of oxy-conformer and oxy-regulator are revisited, and P. W. Hochachka's idea of scope for survival is explored. Finally, how the cardiovascular system contributes to the capacity of fishes to respond to the reduced oxygen availability is considered. Various hands-on recommendations and software (R scripts) are provided for researchers interested in investigating these concepts.

This review and data analysis outline how fish biologists should most reliably estimate the minimal amount of oxygen needed by a fish to support its aerobic metabolic rate (termed standard metabolic rate; SMR). By reviewing key literature, it explains the theory, terminology and challenges underlying SMR measurements in fishes, which are almost always made using respirometry (which measures oxygen uptake, ṀO2 ). Then, the practical difficulties of measuring SMR when activity of the fish is not quantitatively evaluated are comprehensively explored using 85 examples of ṀO2 data from different fishes and one crustacean, an analysis that goes well beyond any previous attempt. The main objective was to compare eight methods to estimate SMR. The methods were: average of the lowest 10 values (low10) and average of the 10% lowest ṀO2 values, after removing the five lowest ones as outliers (low10%), mean of the lowest normal distribution (MLND) and quantiles that assign from 10 to 30% of the data below SMR (q0·1 , q0·15 , q0·2 , q0·25 and q0·3 ). The eight methods yielded significantly different SMR estimates, as expected. While the differences were small when the variability was low amongst the ṀO2 values, they were important (>20%) for several cases. The degree of agreement between the methods was related to the c.v. of the observations that were classified into the lowest normal distribution, the c.v. MLND (C.V.MLND ). When this indicator was low (≤5·4), it was advantageous to use the MLND, otherwise, one of the q0·2 or q0·25 should be used. The second objective was to assess if the data recorded during the initial recovery period in the respirometer should be included or excluded, and the recommendation is to exclude them. The final objective was to determine the minimal duration of experiments aiming to estimate SMR. The results show that 12 h is insufficient but 24 h is adequate. A list of basic recommendations for practitioners who use respirometry to measure SMR in fishes is provided.

Laura Bianucci1,2*, Katja Fennel1, Denis Chabot3, Nancy Shackell4, and Diane Lavoie3 Department of Oceanography, Dalhousie University, 1355 Oxford St, Halifax, NS, Canada Now at Coastal Sciences Division, Pacific Northwest National Laboratory, 1100 Dexter Ave N, Seattle, WA, USA Maurice Lamontagne Institute, Fisheries and Oceans Canada, 850 Route de la Mer, Mont-Joli, QC, Canada G5H 3Z4 Bedford Institute of Oceanography, Fisheries and Oceans Canada, Dartmouth, NS, Canada *Corresponding author: tel: +1 206 528 3413; fax: +1 206 528 3552; e-mail: laura.bianucci@pnnl.gov

Northern shrimp is an important commercial species in the Estuary and Gulf of St. Lawrence. It is usually found at depths > 150 m and thus frequently inhabits hypoxic areas (18–40% saturation) in this region. To evaluate the impact of hypoxia on adult shrimp, males and females were exposed to different levels of dissolved oxygen at two temperatures (5 and 8 °C). Standard and maximal metabolic rates as well as the critical oxygen threshold were measured. In addition, metabolic and antioxidant enzyme activities were measured at 5 °C. Females had a higher critical oxygen threshold than males at both temperatures (15.5 and 22.2 vs. 9.0 and 13.8 at 5 and 8 °C respectively), indicating that they were less tolerant of hypoxia. A decrease in glycolytic and fermentation enzyme activities confirmed this result: in females, severe hypoxia significantly decreased the specific activities of citrate synthase and of enzymes involved in anaerobic biochemical pathways (lactate dehydrogenase, pyruvate kinase, and phosphoenolpyruvate carboxykinase (PEPCK)); in males, only the PEPCK activity decreased significantly while glutathione peroxidase (antioxidant defense) activity increased significantly. In females, severe hypoxia (22% sat.) did not affect the standard metabolic rate but significantly reduced (by ~ 43%) the maximum metabolic rate compared to normoxia. Consequently, aerobic scope was reduced by ~ 58% at 22% sat. compared to normoxia. This suggests that the shrimp's flexibility to respond to metabolic demands, including such activities as vertical migration, foraging, and egg production, could be reduced in hypoxic conditions, especially in females.

Standard metabolic rate, SMR, or the totality of energy-consuming, manipulative and storage chemical reactions undertaken by fish to maintain life is the topic of this article. The factors that account for SMR and measurement theory are considered. Despite decades of effort, there is no universal method for measuring SMR, and various methods and general problems with measuring are discussed. One of the most important factors contributing to differences in SMR is body size. Despite considerable effort, there is still uncertainty in how metabolism scales in fish. Other factors that contribute to SMR such as species, population origin, genetics and season are also discussed.

We have learned much about the impacts of warming on the productivity and distribution of marine organisms, but less about the impact of warming combined with other environmental stressors, including oxygen depletion. Also, the combined impact of multiple environmental stressors requires evaluation at the scales most relevant to resource managers. We use the Gulf of St. Lawrence, Canada, characterized by a large permanently hypoxic zone, as a case study. Species Distribution Models were used to predict the impact of multiple scenarios of warming and oxygen depletion on the local density of three commercially and ecologically important species. Substantial changes are projected within 20-40 years. A eurythermal depleted species already limited to shallow, oxygen-rich refuge habitat (Atlantic cod) may be relatively uninfluenced by oxygen depletion but increase in density within refuge areas with warming. A more stenothermal, deep-dwelling species (Greenland halibut) is projected to lose ~55% of its high-density areas under the combined impacts of warming and oxygen depletion. Another deep-dwelling, more eurythermal species (Northern shrimp) would lose ~4% of its high-density areas due to oxygen depletion alone, but these impacts may be buffered by warming, which may increase density by 8% in the most hypoxic areas, but decrease density by ~20% in the warmest parts of the region. Due to local climate variability and extreme events, and that our models cannot project changes in species sensitivity to hypoxia with warming, our results should be considered conservative. We present an approach to effectively evaluate the individual and cumulative impacts of multiple environmental stressors on a species-by-species basis at the scales most relevant to managers. Our study may provide a basis for work in other low-oxygen regions and should contribute to a growing literature base in climate science, which will continue to be of support for resource managers as climate change accelerates. This article is protected by copyright. All rights reserved.

This study shows that a sharp ecosystem transition in dominant communities during the early 1990s, from finfish to crustaceans, was common to the 2 northernmost Northwest Atlantic ecosystems, the NewfoundlandLabrador shelf (NL) and the northern Gulf of St. Lawrence (nGSL). Fishery and survey data show that populations of Atlantic cod Gadus morhua, typical of most finfish species, collapsed during the late 1980s to early 1990s in both systems, while Green- land halibut Reinhardtius hippoglossoides populations changed little. Biomass of northern shrimp Pandalus borealis increased following the collapse of cod in both systems, likely due, at least in part, to release of predation pressure. Predation appeared to have relatively little effect on bio- mass of snow crab Chionoecetes opilio. Shrimp replaced capelin as the principal prey in the diet of NL cod and nGSL Greenland halibut in the mid-1990s. The contribution of shrimp to the pred- ator diets was generally highest when neither capelin nor other suitable prey (fish or squid) were available. We conclude that the NL and nGSL ecosystems are similar in form and function, differ- ing from Canadian Atlantic ecosystems further south. The implications of a change in predomi- nant forage species from capelin to shrimp in these systems are unknown, but could conveivably include changes in the pathway and efficiency of energy flow.

In an attempt to minimize observer bias, numerical taxonomy methods were used to describe and classify humpback whale sounds. The spectrograms (N = 1255) were digitized into a 16 × 21 binary matrix. The rows were 16 frequencies selected on a logarithmic scale (0.12–8 kHz). The columns were 21 time samples taken every 0.1 s. Each point of the matrix was coded 1 if it lay over part of the sound. Other binary variables were added to code for relative intensity within a sound, frequency modulation and amplitude modulation. The sounds were then compared using the Jaccard similarity coefficient for binary data, and classified with average linkage cluster analysis. This technique produced 115 clusters, which were compared with my aural and visual impressions of the sounds. I agreed with most major categories identified by cluster analysis, but many small clusters had to be fused to other categories. This was partially due to the technique used, and to the complexity of the repertoire under study. Improvements are proposed to further reduce observer bias in classification of sounds, and thus make studies of animal communication performed by different researchers or on different species more easily comparable.

Seasonal changes in energy intake of northwest Atlantic harp seals were modelled and implemented as a Microsoft Excel ™ spreadsheet. Energy intake of adults during the fourth quarter is almost double estimated intake during the second quarter, with intermediate values during the fi rst and third quarters. Reproduction increases female annual energy requirements by 18%, and adds 4% to the estimated population energy intake. The model was sensitive to changes in metabolizable energy, body mass, and the activity factors selected to estimate cost of activity. Changes in blubber conductivity and body composition had intermediate effects, while changes in water and air temperature and activity had little effect on model output. Comparing annual energy intake between a seasonally varying model

In order to have a global view of ecosystem changes associated with the collapse of groundfish species in the Gulf of St. Lawrence during the early 1990s, Ecopath mass-balance models were constructed incorporating uncertainty in the input data. These models covered two ecosystems (northern and southern Gulf of St. Lawrence; NAFO divisions 4RS and 4T), and two time periods (before the collapse, in the mid-1980s, and after it, in the mid-1990s). Our analyses revealed that the ecosystem structure shifted dramatically from one previously dominated by piscivorous groundfish and small-bodied forage species during the mid-1980s to one now dominated only by small-bodied pelagic species during the mid-1990s in both southern and northern Gulf. The species structure in the northern Gulf versus southern Gulf was different, which may explain why these two ecosystems did not recover the same way from the collapse in the early 1990s. Productivity declined in the northern Gulf after the collapse but increased in the southern Gulf. The collapse of groundfish stocks resulted in declines in the mean trophic level of the landings in both the northern and the southern Gulf. Even though fishing mortality was then intentionally reduced, this part of the total mortality was taken up by predation. The temporal changes in the internal structure of both ecosystems are reflected in their overall emergent properties.

Themisto libellula was virtually absent from the St. Lawrence marine system (SLMS) before 1990. Since then, it has become an abundant, full-time resident of this system. Hyperiid amphipods of the genus Themisto are principally carnivorous and represent an essential link in the trophic pathway from secondary production to higher trophic levels. Sampling of T. libellula was car- ried out in the lower St. Lawrence Estuary (LSLE) and the northwest Gulf of St. Lawrence (NWGSL) in the fall of 1998, 2003 and 2004 to study the feeding dynamics and predation impact of this species on mesozooplankton and macrozooplankton communities. Our results showed that T. libellula was an opportunistic predator with a circadian feeding cycle; activity was higher during the second part of the night and the sunrise period. Stomach content analyses showed that these amphipods consumed chiefly copepods, in particular, the copepodite stages CIV and CV of Calanus finmarchicus. Euphausiids, chaetognaths, amphipods and mysids constituted other important prey. Digestion time was estimated at 13 h. The daily ingestion rate of T. libellula was estimated using 2 approaches: (1) stomach fullness index and (2) mean number of prey removed per unit of time and converted to prey biomass using the stage-species dry masses of each prey item. We found that the daily ingestion rate of T. libellula ranged from 6.32 to 16.82% of body dry mass per day in both study areas (LSLE and NWGSL). Concerning predation impact, T. libellula consumed between 0.14 and 1.79% of the com- bined mesozooplankton and macrozooplankton standing stock per day and between 0.43 and 2.48% of the C. finmarchicus standing stock. Themisto libellula may thus exert a significant control on the mesozooplankton and macrozooplankton communities in the SLMS through direct predation.

Atlantic cod Gadus morhua stomach contents (n = 30 973, including 28 377 non-empty stomachs) and morphometric measurements on live snow crab Chionoecetes opilio and cod were examined to assess the predator-prey relationship between these 2 species. The most common snow crab instars found in cod stomachs were III and V (~6 to 8 and ~12 to 16 mm carapace width (CW), respectively) in the northern Gulf of St. Lawrence (GSL) and VI and VII (~17 to 23 and ~23 to 31 mm CW, respectively) in the southern GSL. A significant positive relationship was found between cod length and the largest and smallest CW of snow crab ingested by cod. Positive relationships were also found between gape width and body length in cod and between 3 measures of size (maximum span, width at rest, length at rest) and CW in snow crab. Snow crab length at rest was closely related to cod gape width, suggesting that the largest snow crab ingested by cod must be attacked from the side. There appears to be a plateau at 65.1 mm in the relationship between maximum snow crab CW and cod length, caused by the absence of large (adolescent and adult) male snow crab in cod stomachs. Other studies have found recently moulted, soft-shell snow crabs in cod stomachs, but this appears to be rare. Thus, snow crabs are susceptible to predation by cod mostly for the first 4 yr of post- settlement in the GSL. Any effect of cod predation on the snowcrab fishery would be felt 6 to 11 yr later, given growth models established for the GSL.

The impact of temperature on oxygen consumption (MO 2 ) of Pandalus borealis was measured in the laboratory for juvenile, male and female shrimp at 2, 5 and 8 °C, the temperature range where P. borealis is generally found in the Northwest Atlantic. Measurements of MO 2 were taken every 60 min over 7–10 days on 0.22 to 13.80 g shrimp. These long-term measurement periods insured acclimation to the respirometers and allowed calculation of standard metabolic rate of individuals (SMR ind ). SMR ind was linearly related to body mass (log transformed data) and the mass-exponent for the species was 0.56. The effects of wet body mass ( W w ) and temperature ( T ) on SMR ind were described by: SMR ind = 10 (0.57 log ( W w )+0.04 T −1.21) , with SMR ind expressed in mg O 2 h − 1 , W w in g and T in °C. This model explained 92% of the variability in SMR ind . Mass-specific MO 2 adjusted for a 5 g shrimp (SMR 5g ) was not influenced by developmental stage indicating that the regression model for SMR ind was valid regardless of variations in size at maturity and sex transition. Routine and active metabolic rates followed the same variation pattern in relation to temperature and developmental stage than SMR. Finally, thermal coefficients ( Q 10 ) were in the normal range found for crustaceans, Q 10 values varying from 1.73 to 4.97.

Mass-balance models have been constructed using inverse methodology for the northern Gulf of St. Lawrence for the mid-1980s, the mid-1990s, and the early 2000s to describe ecosystem structure, trophic group interactions, and the effects of fishing and predation on the ecosystem for each time period. Our analyses indicate that the ecosystem structure shifted dramatically from one previously dominated by demersal (cod, redfish) and small-bodied forage (e.g., capelin, mackerel, herring, shrimp) species to one now dominated by small-bodied forage species. Overfishing removed a functional group in the late 1980s, large piscivorous fish (primarily cod and redfish), which has not recovered 14 years after the cessation of heavy fishing. This has left only marine mammals as top predators during the mid-1990s, and marine mammals and small Greenland halibut during the early 2000s. Predation by marine mammals on fish increased from the mid-1980s to the early 2000s while predation by large fish on fish decreased. Capelin and shrimp, the main prey in each period, showed an increase in biomass over the three periods. A switch in the main predators of capelin from cod to marine mammals occurred, while Greenland halibut progressively replaced cod as shrimp predators. Overfishing influenced community structure directly through preferential removal of larger-bodied fishes and indirectly through predation release because larger-bodied fishes exerted top-down control upon other community species or competed with other species for the same prey. Our modelling estimates showed that a change in predation structure or flows at the top of the trophic system led to changes in predation at all lower trophic levels in the northern Gulf of St. Lawrence. These changes represent a case of fishery-induced regime shift.

Mass-balance models, using inverse methodology, were applied to the southern Gulf of St. Lawrence for the mid-1980s and the mid-1990s to describe ecosystem structure, trophic group interactions, and the effects of fishing and predation on the ecosystem for periods preceding and following the collapse of groundfish stocks in this area. These models were used to determine how the ecosystem changed, and whether its structure and functioning were affected by the observed changes in key species between the two time periods. Our analyses indicate that the ecosystem structure shifted dramatically from one previously dominated by piscivorous groundfish and small-bodied forage species (e.g., capelin, herring, and shrimp) in similar proportions to one now dominated by small-bodied forage species. Overfishing removed a functional group, large-bodied demersal predators that has not been replaced 12 years after the cessation of heavy fishing, and left marine mammals such as seals and cetacea as top predators of many species (especially fishes) during the mid-1990s. Predation by marine mammals on fish increased from the mid-1980s to the mid-1990s while predation by large fish on fish decreased. A change in the prey of seals from juvenile cod to capelin occurred between the models for the mid-1980s and the mid-1990s consistent with observed shifts in the abundance of the two prey species between the two time periods. These major changes were accompanied by a decrease in total catches and a transition in landings from long-lived and piscivorous groundfish toward planktivorous pelagic fish and invertebrates.

Larvae of the northern shrimp Pandalus borealis (Kroyer) are pelagic. In the Estuary and Gulf of St. Lawrence, Canada, the early stages are found in the upper 25-m of the water column in spring and early summer and are expected to experience a range of water temperatures from as low as 0°C to as high at 6°C. Little is known of the impact of water temperature on metabolic requirements of northern shrimp larvae. In this study, routine respiration (VO2), maximum respiration (electron transport system activity, ETSA) and metabolic scope for growth (MS, ETSA–VO2) of northern shrimp larvae were measured as a function of temperature (3, 5 and 8°C), developmental stage (I–V at 3°C, I–VII at 5°C and 8°C) and growth rate in dry mass. After logarithmic transformation, all three metabolic variables were linearly related to dry mass. The increase in VO2 with body mass was faster at 5°C than at 3 or 8°C, whereas with ETSA this increase was slower. As a result, MS increased more slowly with dry mass at 5°C than at 3 and 8°C. However, MS did not limit growth in this study, since it explained only 39% of the variability in growth. All three metabolic variables as well as growth varied together as a function of temperature and ontogeny. Q10 of all three metabolic variables ranged from 1.6 and 2.2 for stages I–V larvae, except for VO2 at stage I (3.9) and stage III (2.9).

Northern shrimp Pandalus borealis (Kroyer) larvae hatch in the northern Gulf of St. Lawrence from early May to the end of June, and larval development occurs over a range of relatively cold water temperatures. Because of the long duration of the pelagic phase and the difficulty of sampling all successive larval stages at sea, we used laboratory experiments to assess the effects of water temperature on larval development and growth. In spring 2000, P. borealis larvae were reared from hatching to the first juvenile stages (i.e., stage VI and VII) at three temperatures (3, 5, and 8°C) representing conditions similar to those in spring in the northern Gulf of St. Lawrence. Larval development and growth were dependent on temperature, with longer duration and smaller size (cephalothorax length, CL, and dry mass, DM) at 3°C relative to the 5 and 8°C treatments. There were no significant differences in the morphological characters of the different stages among treatments, indicating that regular moults occurred at each temperature. The results suggest a negative impact of cold temperatures (lower intra-moult growth rates and smaller size) and, possibly, higher cumulative mortality due to longer development time that could affect the success of cohorts at sea. However, CL and DM for stage III and later larvae were smaller than those of larvae identified at the same developmental stage in field locations. It is possible that the diet offered to larvae in this experiment (Artemia nauplii, either newly hatched nauplii or live adults, depending on the developmental stage) was not optimal for growth, even though it is known to support successful P. borealis larval development. In the field, there is the possibility that phytoplankton contributes to the larval diet during the first stages and stimulates development of the digestive glands. Furthermore, the nutritional quality of the natural plankton diet (e.g., high protein content, fatty acid composition) might be superior and favourable to higher growth rates even at lower temperatures.

Final thermal preferendum (T) experiments were conducted in a horizontal thermal gradient tank from the beginning of August 2001 to mid-November 2001 using Atlantic cod Gadus morhua from 6.5 to 79.0 cm fork length (L F ). The value of T varied significantly (P

Mass-balance models using inverse methodology have been constructed for the northern Gulf of St. Lawrence ecosystem in the mid-1980s, before the groundfish collapse. The results highlight the effects of the major mortality sources (fishing, predation, and other sources of mortality) on the fish and invertebrate communities. Main predators of fish were large cod (Gadus morhua) followed by redfish (Sebastes spp.), capelin (Mallotus villosus), and fisheries. Large cod were the most important predator of small cod, with cannibalism accounting for at least 44% of the mortality of small cod. The main predators of large cod were harp (Phoca groenlandica) and grey (Halichoerus grypus) seals. However, predation represented only 2% of total mortality on large cod. Mortality other than predation dominated the mortality processes at 52% of the total, while the fishery represented 46%. Tests were performed to identify possible sources of this unexplained mortality. The only way to significantly reduce unexplained mortality on large cod in the model was to increase landings of large cod above those reported. This suggests that fishing mortality was substantially underestimated in the mid-1980s, just before the demise of a cod stock that historically was the second largest in the northwest Atlantic.

Diel feeding patterns of herring Clupea harengus and mackerel Scomber scombrus in the southern Gulf of St Lawrence were examined based on samples obtained by midwater trawling between 19 and 26 June 2001. Within 3 h time periods, stomach contents tended to be more similar between fish from the same tow than between fish from different tows. Thus, in contrast to previous diet studies, which have used individual fish stomachs as independent observations, tow was used as the experimental unit in statistical analyses in this study. Diel patterns in stomach fullness were identified using generalized additive models. Two peaks in stomach fullness occurred for herring, one in the morning and the other in the evening. Mackerel showed an increase in feeding intensity throughout the day with a peak in mid-afternoon. The diel changes in stomach contents suggested rapid gastric evacuation rates for both species, especially for herring. The estimate of the instantaneous evacuation rate for herring was twice that for mackerel. Calanus copepods (mainly C. hyperboreus), fishes (mainly capelin Mallotus villosus) and euphausiids were the main prey found in the stomachs of both species. Calanus copepods dominated the diet of herring regardless of time period. They also dominated the diet of mackerel during the late afternoon, evening and night while fishes and euphausiids were dominant during the morning and early afternoon. These diel patterns emphasize the need for sampling throughout the day and night in order to estimate ration and diet composition for bioenergetic and ecosystem models.

Atlantic cod were food-deprived for a period of 84 days at three temperatures (2, 6, 10 � C), and changes in the liver, gonads and somatic weights, and muscle and liver water contents were monitored and compared with changes observed in wild cod over winter in the northern Gulf of St. Lawrence. Total lack of food during the period January–April would have caused condition to decline to a level at which very high mortality takes place. Actual changes in condition in wild cod were less than predicted from the laboratory experiments except during the period April–May at the onset of spawning. Thus, wild cod were able to meet part of the metabolic costs during winter through occasional feeding, as confirmed by stomach content data. We conclude that previous estimates of natural mortality associated with poor condition in spring were not biased by the selective mortality of poor-condition fish in winter. Crown Copyright 2003 Published by Elsevier Ltd on behalf of International Council for the Exploration

Pinnipeds have marked seasonal changes in body condition, accumulating energy re- serves prior to the reproduction period, and depleting these reserves during the breeding and molt- ing periods. This cycle is not well described in male harp seals Phoca groenlandica. We obtained mor- phometric measurements for 3167 Northwest Atlantic male harp seals sampled between 1979 and 1995. The part of the yearly cycle covered in this study (November to May) was divided into short blocks around median dates called 'periods'. For each period, a growth curve was calculated for body mass, sculp mass, core mass and standard length. These sequential growth curves were used to cal- culate changes in size-at-age between periods. In addition, seasonal changes in 3 condition indices (general, sculp and core condition), in blubber thickness and in the ratio of sculp to total mass were analyzed using locally weighted regressions. Male harp seals returned from their high-latitude feed- ing grounds heavier, in better condition, and with a thicker blubber layer than when they left the area in the spring. However, maxima in length, mass, condition and blubber thickness were not observed until the February 22 period (February 12 to March 4), or in the case of seals younger than 5 yr, some- time in March. Mass losses associated with the rut began between the periods February 22 and March 15 (March 5 to 25) and were most pronounced in seals older than 10 yr. About 66% of the losses in mass came from the core during this period. We estimated that males lost 39.4 kg during the rut (1.16 kg d -1 or 0.78% of initial mass d -1 , 44% from sculp and 56% from core) and incurred energy expenditures of 840 MJ (24.7 MJ d -1 ). This corresponds to twice the standard metabolic rate (SMR), and suggests that males are feeding during the rut. All males lost mass in April, and again in May. They began to molt April 19 period (April 16 to 22). Fewer than half the seals had completed the shed- ding of old hair when the study ended, in the May 10 period. Rates of mass loss during the molt varied from 1 to 1.8 kg d -1 , depending on the age of the seals. Minima of length, mass, condition and blubber thickness were observed in May. Molting appears to be a period of high energy expenditures (3 to 5 SMR) despite the low levels of activity observed at this time. This study revealed that length also changes seasonally: fat seals in February were longer than lean seals of the same age in late April.

The spatial distribution of Atlantic cod Gadus morhua in the southern Gulf of St. Lawrence changed substantially between the 1970s and the 1980s. One proposed explanation for this phenomenon is that the fish were occupying different temperature zones as a result of changes in food availability brought about by changes in the species' abundance. To evaluate one key aspect of this hypothesis, we determined the final thermal preferendum of Atlantic cod in relation to food ration. After 6–15 weeks of conditioning to one of three predetermined rations, 72 fish 40–50 cm long were left to behaviorally thermoregulate three at a time in a horizontal thermal gradient for 45 h. Food ration produced significant differences among the condition indicators of the three groups. A small but significant difference (analysis of variance, P < 0.05) in final thermal preferendum was found between fish fed intermediate and low rations. Omission of the data from the first 3 weeks of the experiment, which showed signs of...

Using a sample of 150 female and 141 male foetal Northwest Atlantic harp seals (Phoca groenlandica) collected between October and February of 1980-1995, we determined that the allometric relationship between total body mass and standard length did not differ between sexes and did not differ from the relationship calculated for foetal harp seals from the study of Stewart and co-workers (R.E.A. Stewart, B.E. Stewart, D.M. Lavigne, and G.W. Miller. 1989. Can. J. Zool. 67: 2147-2157). Combining the data for foetuses from both studies, the exponent of the mass-length relationship was 2.75, significantly different from 3. Except for very small foetuses (1/2.75 increased linearly as gestation progressed. Equations are provided to estimate standard length and total mass of foetuses in the period August-February and to estimate sculp mass, core mass, and axillary girth from length for the period October-February. The most reliable estimate of implantation date (day 223, 11 August) was obtained by regressing sampling date1/2 on mass1/2.75.

Growth in length and mass, improvements in condition, as well as final condition of c. 700 g Atlantic cod Gadus morhua were significantly less at 45% and 56% O2 saturation than at 65%, 75%, 84% and 93% O2 saturation. Hypoxia decreased food consumption. In turn, food consumption explained 97% of the variation in growth. Conversion efficiency varied slightly, but significantly, with level of dissolved O2, except that the group reared at 93% O2 had a lower than expected conversion efficiency. Slow growth in low O2 was not due to increased activity, because activity decreased in hypoxia. In the Gulf of St Lawrence, waters deeper than 200 m usually are

Oxygen saturation levels that killed 50 and 5% of cod Gadus morhua over 96 h averaged 21·2 and 27·7%, respectively. No fish survived at 10% saturation and only a few survived at 16% saturation, whereas no mortality occurred at 34 and 40% oxygen saturation. Since metabolic rate and oxygen consumption increase with increasing temperature, we hypothesized that cod would be less tolerant to hypoxic conditions at 6 than at 2° C. However, temperature (2 and 6° C) had no measurable impact on cod survival. Small (mean & S.D.; 45·2 ± 4·2 cm) and large (57·5 ± 3·8 cm) cod had the same tolerance to hypoxia. At the end of the experiments, hypoxia had a significant effect on blood haematocrit, mean cellular haemoglobin content, liver lactate, plasma glucose and plasma lactate, but accounted for only a small fraction (< 10%) of the variation, except for plasma lactate which exhibited a strong response with concentrations increasing progressively with decreasing levels of oxygen saturation. Temperature had a significant effect on most variates in normoxia and hypoxia. Variates also affected by oxygen level showed significant interactions between oxygen and size or temperature effects. However, these interactions accounted for only a small proportion of the variation. Physiological parameters indicated that extending the duration of our tests beyond 96 h would not have changed our estimates of the lethal thresholds. Hypoxic conditions are a permanent feature of the deep waters of the Gulf of St Lawrence. This study shows that a significant portion of the benthic habitats in the Gulf are uninhabitable for cod which would be expected to avoid waters below 28% oxygen saturation.

This study examined the effects of prey exoskeleton characteristics on gastric evacuation patterns in Atlantic cod Gadus morhua. Three distinct stages were highlighted in the gastric evacuation of crustacean prey characterized by a robust exoskeleton. The experiments confirmed that the three shrimp species, Pandalus borealis, Pandalus montagui and Eualus macilentus, and the crab Chionoecetes opilio, were evacuated from the stomach at different rates. The duration of all stages increased with increasing ash (and carbonate) content of the fresh prey. Thickness, chemical composition and morphology of the prey exoskeleton all affected gastric evacuation: duration of initial delay, overall evacuation rate and a decreased evacuation rate at the end of the process. The power exponential function (PEF), with its shape parameter, described the course of evacuation for these prey types well, especially the initial delay. The PEF does not, however, allow describing evacuation by the current stomach content mass independent of meal size, which limits its usefulness in estimating consumption rates of wild G. morhua. To predict and describe gastric evacuation of prey with a robust exoskeleton, it is therefore suggested that the square-root function be expanded with an initial lag phase, coupled to the mechanistically based cylinder model of gastric evacuation.

Greenland halibut (Reinhardtius hippoglossoides), especially juveniles, are frequently found in severely hypoxic areas (18%–25% saturation) of the St. Lawrence Estuary. We investigated the tolerance of this species to hypoxia and evaluated the consequences of low oxygen levels on metabolic capacity. At 5 °C, juveniles had a higher critical oxygen threshold than adults (15% versus 11% saturation), indicating that they were less tolerant to hypoxia. Severe hypoxia (19% saturation) did not affect the juveniles' standard metabolic rate but significantly reduced (by 55%) their maximum metabolic rate compared with normoxia. Consequently, the aerobic scope was reduced by 72% in hypoxia compared with normoxia. In juveniles, severe hypoxia increased the duration of digestive processes. The decrease in aerobic scope in hypoxia and the determination of critical oxygen threshold at a saturation level close to actual field dissolved oxygen values strongly suggest that juveniles from the St. Lawrence Estuary are living at the edge of their metabolic capacity. Consequently, the growth and distribution of Greenland halibut could be affected if there are further declines in dissolved oxygen availability.

This paper reports an evaluation study of a pilot project held in a “CLSC” (Local Community Health Centre) located in a rural community. The intervention design is based on multidisciplinary teams created to meet specific local community needs. Mental health intervention is part of community-based approach open to all kinds of requests for support. Practitioners in the field give support not only to the individual with a mental health problem, but also to existing self-help networks in the community. The intervention is also designed to change the relationship between a professional, seen as the one who provides services, and a client, defined as a consumer. The term “client” gains a wider meaning, and collaborative work with informal self-help networks induces a closer relationship between the community and the practitioner. The practitioner is more open to local community values and adjusts his or her practice to match variable contexts. The evaluation study has expanded knowledge on this type of intervention design, on its impact upon service demand, intervention modalities, and service management and organization. This project was coupled with a similar project in an urban environment and was financed by the Health and Social Services Ministry in Québec.