68 results on '"Depince, Alexandra"'
Search Results
2. One Alternative to Germ Cells Cryopreservation: Cryobanking of Somatic Cells in Sturgeon
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Labbe, Catherine, Depince, Alexandra, Le Bail, Pierre-Yves, Williot, Patrick, Williot, Patrick, editor, Rochard, Eric, editor, Desse-Berset, Nathalie, editor, Kirschbaum, Frank, editor, and Gessner, Jörn, editor
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- 2011
- Full Text
- View/download PDF
3. Fin cell cryopreservation and fish reconstruction by nuclear transfer stand as promising technologies for preservation of finfish genetic resources
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Chenais, Nathalie, Depince, Alexandra, Le Bail, Pierre-Yves, and Labbe, Catherine
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- 2014
- Full Text
- View/download PDF
4. Characterization of a new embryonic cellular model for nuclear transfer in fish: the embryoid body
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Rouillon, Charlène, Depince, Alexandra, Le Bail, Pierre-Yves, Labbé, Catherine, Laboratoire de Physiologie et Génomique des Poissons (LPGP), Institut National de la Recherche Agronomique (INRA)-Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique ), CR is recipient of an INRA-PHASE and Région Bretagne fellowship. This work is funded by the French CRB Anim project «Investissements d’avenir», ANR-11-INBS-0003, ANR-11-INBS-0003,CRB-Anim,Réseau de Centres de Ressources Biologiques pour les animaux domestiques(2011), Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique )-Institut National de la Recherche Agronomique (INRA), ProdInra, Archive Ouverte, and Infrastructures - Réseau de Centres de Ressources Biologiques pour les animaux domestiques - - CRB-Anim2011 - ANR-11-INBS-0003 - INBS - VALID
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[SDV] Life Sciences [q-bio] ,reprograming ,DNA methylation ,[SDV]Life Sciences [q-bio] ,embryonic structures ,education ,carassius auratus ,trichostatin A ,transcription - Abstract
Characterization of a new embryonic cellular model for nuclear transfer in fish: the embryoid body. 7. International Workshop on the Biology of Fish Gametes
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- 2019
5. Comparative analysis of global DNA methylation in spermatozoa from fish, molluscs, bird and mammals: effect of cryopreservation
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Depince, Alexandra, Jammes, Helene, Labbé, Catherine, Laboratoire de Physiologie et Génomique des Poissons (LPGP), Institut National de la Recherche Agronomique (INRA)-Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique ), Biologie du Développement et Reproduction (BDR), École nationale vétérinaire d'Alfort (ENVA)-Institut National de la Recherche Agronomique (INRA), Funding support of 'Investissements d’Avenir' ANR-11-INBS-0003 (CRB-Anim 2013-2019), ANR-11-INBS-0003,CRB-Anim,Réseau de Centres de Ressources Biologiques pour les animaux domestiques(2011), Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique )-Institut National de la Recherche Agronomique (INRA), Biologie du développement et reproduction (BDR), École nationale vétérinaire d'Alfort (ENVA)-Institut National de la Recherche Agronomique (INRA)-Centre National de la Recherche Scientifique (CNRS), and Funding support of 'Investissements d’Avenir'ANR-11-INBS-0003 (CRB-Anim 2013-2019)
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urogenital system ,[SDV]Life Sciences [q-bio] ,education ,gamete ,reproductive and urinary physiology ,epigenetic ,biotechnology - Abstract
Comparative analysis of global DNA methylation in spermatozoa from fish, molluscs, bird and mammals: effect of cryopreservation. 7. International Workshop on the Biology of Fish Gametes
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- 2019
6. Changes in sperm cryopreservation procedure for industrial needs
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Labbé, Catherine, Delhomme, Guy, Depince, Alexandra, Gavin-Plagne, Lucie, Goardon, Lionel, Kica, Stéphanie, Leboucher, Richard, Morvezen, Romain, Na, Li, Laboratoire de Physiologie et Génomique des Poissons (LPGP), Institut National de la Recherche Agronomique (INRA)-Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique ), IMV Technologies, Pisciculture Expérimentale INRA des Monts d'Arrée (PEIMA), Institut National de la Recherche Agronomique (INRA), Syndicat des Sélectionneurs Avicoles et Aquacoles Français (SYSAAF), Work funded by the FEAMP BIOGERM project and by the PIA CRB Anim (ANR-11-INBS-0003), ANR-11-INBS-0003,CRB-Anim,Réseau de Centres de Ressources Biologiques pour les animaux domestiques(2011), Syndicat des Sélectionneurs Avicoles et Aquacoles Français, and Work funded by the FEAMP BIOGERM (2017, mesure 49) project and by the PIA CRB Anim (ANR-11-INBS-0003
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Cryoconservation ,raft ,urogenital system ,[SDV]Life Sciences [q-bio] ,education ,MeOH ,Poisson ,Digitcool ,Rainbow trout ,Cryoconservation du sperme ,neomale ,Oncorhynchus mykiss ,Sperme ,sense organs ,skin and connective tissue diseases ,DMSO ,health care economics and organizations - Abstract
Changes in sperm cryopreservation procedure for industrial needs. 7. International Workshop on the Biology of Fish Gametes
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- 2019
7. Cryoconservation du sperme de salmonidés : évolution des pratiques et amélioration des fécondances
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Labbé, Catherine, Delhomme, Guy, Depince, Alexandra, Gavin-Plagne, Lucie, Goardon, Lionel, Kica, Stéphanie, Leboucher, Richard, Morvezen, Romain, Li, Na, Laboratoire de Physiologie et Génomique des Poissons (LPGP), Institut National de la Recherche Agronomique (INRA)-Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique ), IMV-Technologies, Pisciculture Expérimentale INRA des Monts d'Arrée (PEIMA), Institut National de la Recherche Agronomique (INRA), Syndicat des Sélectionneurs Avicoles et Aquacoles Français, ANR-11-INBS-0003 & Projet BIOGERM Fonds Européens des Affaires Maritimes et de la Pêche (FEAMP), Syndicat des Sélectionneurs Avicoles et Aquacoles Français (SYSAAF), Institut Technique de l'Aviculture et des Elevages de Petits Animaux (ITAVI). FRA., and ANR-11-INBS-0003,CRB-Anim,Réseau de Centres de Ressources Biologiques pour les animaux domestiques(2011)
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fish ,salmonidae ,poisson ,fécondance des spermatozoides ,évolution des pratiques ,salmonids ,sperme ,[SDV]Life Sciences [q-bio] ,cryofixation ,semen ,cryoconservation ,congelation du sperme ,fécondance - Abstract
Session : Reproduction; Cryoconservation du sperme de salmonidés : évolution des pratiques et amélioration des fécondances. 6. Journées de la Recherche Filière Piscicole
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- 2019
8. Régénération de lignées de truite par transplantation de cellules souches germinales
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Goupil, Anne-Sophie, Krieg, Francine, Maouche, Ahmed, Depince, Alexandra, Duret, Cécile, Dechamp, Nicolas, Goardon, Lionel, Quillet, Edwige, Le Gac, Florence, Lareyre, Jean-Jacques, Laboratoire de Physiologie et Génomique des Poissons (LPGP), Institut National de la Recherche Agronomique (INRA)-Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique ), Génétique Animale et Biologie Intégrative (GABI), Institut National de la Recherche Agronomique (INRA)-AgroParisTech, Pisciculture Expérimentale INRA des Monts d'Arrée (PEIMA), Institut National de la Recherche Agronomique (INRA), Institut Technique de l'Aviculture et des Elevages de Petits Animaux (ITAVI). FRA., and European Project: 652831,H2020,H2020-INFRAIA-2014-2015,AQUAEXCEL2020(2015)
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[SDV.GEN]Life Sciences [q-bio]/Genetics ,[SDV.GEN.GA]Life Sciences [q-bio]/Genetics/Animal genetics ,[SDV]Life Sciences [q-bio] ,[INFO]Computer Science [cs] ,ComputingMilieux_MISCELLANEOUS - Abstract
Session : Reproduction; National audience
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- 2019
9. Analyse comparée du niveau de méthylation globale des spermatozoïdes chez des mâles de 9 espèces d’embranchements différents
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Depince, Alexandra, Jammes, Hélène, Labbé, Catherine, Laboratoire de Physiologie et Génomique des Poissons (LPGP), Institut National de la Recherche Agronomique (INRA)-Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique ), Biologie du Développement et Reproduction (BDR), École nationale vétérinaire d'Alfort (ENVA)-Institut National de la Recherche Agronomique (INRA), Université Paris Saclay (COmUE), and ANR-11-INBS-0003 (CRB-Anim 2013-2019)
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[SDV]Life Sciences [q-bio] ,[INFO]Computer Science [cs] ,ComputingMilieux_MISCELLANEOUS - Abstract
National audience
- Published
- 2019
10. Characterization of early embryonic cellular defects after somatic cell nuclear transfer in fish
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Rouillon, Charlène, Depince, Alexandra, Chenais, Nathalie, Le Bail, Pierre-Yves, Labbé, Catherine, Laboratoire de Physiologie et Génomique des Poissons (LPGP), and Institut National de la Recherche Agronomique (INRA)-Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique )
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fish ,transfer nuclear ,early embryonic cellular ,[SDV]Life Sciences [q-bio] ,somatic cell - Abstract
International audience; Cryopreservation of genetic resources is of major interest for the security of biodiversity and the sustainability of the agronomic industry. Cryopreservation of somatic cells is one means of preserving the genome of both parents, but regeneration of functional breeders requires the mastering of somatic cell NT (SCNT). In mammals, SCNT consists of injecting the somatic nuclei into enucleated recipient oocytes to obtain clones that bear the genome of the donor. Cloning in fish presents some advantages over cloning in mammals: oocytes are produced by the hundreds, and development is external. Moreover, embryonic genome activation takes place after 10 mitoses only. These features are favourable to a better understanding of cellular reprogramming of the somatic cell. Nonenucleated oocytes are often used for SCNT, which allows the production of 2n clones bearing only the donor genome when the maternal chromatin is spontaneously removed by an unknown mechanism (25% of cases). This ability can help us understand the fate of the maternal chromatin and the role of its surrounding factors on the cellular behaviour of the somatic cell during the first developmental steps of embryonic development: meiosis resumption (MR) and mitosis. In this study, fin somatic cells and mature oocytes were obtained from a 2-year-old goldfish (Carassius auratus). For SCNT, the whole fin cell was injected into the sperm entry site, and the oocyte was activated by water contact after 30 min to begin the development. Somatic and maternal chromatin behaviour during MR and early mitosis were characterised by immunofluorescence (Hoechst/Vybrant labelling for chromatin identification and α-tubulin for spindle organisation). Variability in chromatin condensation was observed after the somatic cell injection before the activation. Clone analysis (n = 16) revealed that some of them presented a condensed somatic nuclei (71%) and others presented a decondensed nuclei (29%). After activation, most clones underwent normal polar body extrusion of the maternal chromatin (66%, n = 69/95 clones v. 96%, n = 96/100 controls oocytes) without extrusion of a somatic polar body. Afterward, clones that presented a first symmetric division (n = 14/35) were analysed at the 2-cells stage and compared with fertilized embryos (n = 7). The maternal chromatin was observed as fragmented on the cleavage furrow (79%), where it cannot contribute to the development. Spindle defects were observed in 50% of clone cells (v. 0% in controls), such as multicentrosomal spindles, chromosome misalignment, abnormal segregation, and DNA fragmentation. To conclude, those clone (2n or 3n) defects are probably due to the chromatin condensation observed before activation. The fish oocyte volume did not allow the MR of the condensed somatic chromatin, and that may induce an abnormal anaphase and the following clone developmental defects.
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- 2019
11. 7th International Workshop on the Biology of Fish Gametes - 2-6 Sept. 2019, Rennes France. Abstract book
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Laurent, Audrey, Depince, Alexandra, Laboratoire de Physiologie et Génomique des Poissons (LPGP), and Institut National de la Recherche Agronomique (INRA)-Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique )
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[SDV]Life Sciences [q-bio] ,[INFO]Computer Science [cs] ,ComputingMilieux_MISCELLANEOUS - Abstract
International audience
- Published
- 2019
12. Somatic cell nuclear transfer in non-enucleated goldfish oocytes: understanding DNA fate during oocyte activation and first cellular division
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Rouillon, Charlène, Depince, Alexandra, Chenais, Nathalie, Le Bail, Pierre-Yves, Labbé, Catherine, Laboratoire de Physiologie et Génomique des Poissons (LPGP), Institut National de la Recherche Agronomique (INRA)-Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique ), This work has benefited from the Tefor Fish Phenotyping Platform at the INRA LPGP, Rennes (ANR-II-INBS-0014). This work was funded by the French CRB Anim project, ANR-11-INBS-0003. C.Rouillon was recipient of an INRA PHASE and Région Bretagne PhD fellowship, ANR-11-INBS-0003,CRB-Anim,Réseau de Centres de Ressources Biologiques pour les animaux domestiques(2011), and ANR-11-INBS-0014,TEFOR,Transgenèse pour les Etudes Fonctionnelles sur les Organismes modèles(2011)
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cell division ,clone cellulaire ,Nuclear Transfer Techniques ,Embryology ,nuclear transfer ,reprogrammation nucléaire ,[SDV]Life Sciences [q-bio] ,clone cells ,lcsh:Medicine ,Spindle Apparatus ,eye enucleation ,division cellulaire ,dna ,Article ,Chromosomes ,reproduction ,histologie ,énucléation ,poisson ,cyprinidae ,Animals ,reprogramming of the genome ,goldfish ,immunofluorescence ,lcsh:Science ,genome ,Metaphase ,cell division cycle ,fish ,génome ,lcsh:R ,carassius auratus ,indirect fluorescent antibody techn ,adn ,cryoconservation ,cycle cellulaire ,poisson rouge ,transfert nucléaire ,cryofixation ,Oocytes ,lcsh:Q ,Cloning - Abstract
Nuclear transfer consists in injecting a somatic nucleus carrying valuable genetic information into a recipient oocyte to sire a diploid offspring which bears the genome of interest. It requires that the oocyte (maternal) DNA is removed. In fish, because enucleation is difficult to achieve, non-enucleated oocytes are often used and disappearance of the maternal DNA was reported in some clones. The present work explores which cellular events explain spontaneous erasure of maternal DNA, as mastering this phenomenon would circumvent the painstaking procedure of fish oocyte enucleation. The fate of the somatic and maternal DNA during oocyte activation and first cell cycle was studied using DNA labeling and immunofluorescence in goldfish clones. Maternal DNA was always found as an intact metaphase within the oocyte, and polar body extrusion was minimally affected after oocyte activation. During the first cell cycle, only 40% of the clones displayed symmetric cleavage, and these symmetric clones contributed to 80% of those surviving at hatching. Maternal DNA was often fragmented and located under the cleavage furrow. The somatic DNA was organized either into a normal mitotic spindle or abnormal multinuclear spindle. Scenarios matching the DNA behavior and the embryo fate are proposed.
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- 2019
13. Somatic cell nuclear transfer in non-enucleated goldfish oocytes: understanding DNA fate during meiosis resumption and first cellular division
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Rouillon, Charlène, Depince, Alexandra, Chenais, Nathalie, Le Bail, Pierre-Yves, and Labbé, Catherine
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énucléation ,poisson ,cellule somatique ,reprogrammation nucléaire ,transfert nucléaire ,développement embryonnaire ,cyprinidae ,carassius auratus ,clonage cellulaire ,survie embryonnaire ,ovocyte ,immunofluorescence ,cryoconservation - Abstract
Nuclear transfer consists in injecting a somatic nucleus carrying valuable genetic information into a recipient oocyte to sire a diploid offspring who bears the genome of interest. It requires that the oocyte (maternal) DNA is removed. In fish, because enucleation is difficult to achieve, non-enucleated oocytes are often used and disappearance of the maternal DNA was reported in some clones. The present work explore which cellular events explain spontaneous erasure of maternal DNA, as mastering this phenomenon would circumvent the painstaking procedure of fish oocyte enucleation. The fate of the somatic and maternal DNA during meiosis resumption and first cell cycle was studied using DNA labeling and immunofluorescence in goldfish clones. Maternal DNA was always found as an intact metaphase within the oocyte, and polar body extrusion was minimally affected after meiosis resumption. During the first cell cycle, only 40 % of the clones displayed symmetric cleavage, and these symmetric clones contributed to 80 % of those surviving at hatching. Maternal DNA was often fragmented and located under the cleavage furrow. The somatic DNA was organized either into a normal mitotic spindle or abnormal multinuclear spindle. Scenarios matching the DNA behavior and the embryo fate are proposed.
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- 2019
14. Meiosis resumption and early mitosis after somatic cell nuclear transfer in non-enucleated goldfish oocytes
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Rouillon, Charlène, Depince, Alexandra, Le Bail, Pierre-Yves, Labbé, Catherine, Laboratoire de Physiologie et Génomique des Poissons (LPGP), Institut National de la Recherche Agronomique (INRA)-Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique ), Instituto Nacional de Pesquisas da Amazônia (INPA). Manaus, BRA., Laboratoire de Physiologie et Génomique des Poissons ( LPGP ), and Institut National de la Recherche Agronomique ( INRA ) -Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique )
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fish ,nuclear transfer ,[ SDV ] Life Sciences [q-bio] ,[SDV]Life Sciences [q-bio] ,carassius auratus ,embryo ,ovocyte ,cryoconservation ,méïose ,reproduction ,transfert nucléaire ,cellule somatique ,poisson ,cyprinidae ,embryon ,cryofixation ,somatic cell ,goldfish - Abstract
Introduction: The cryopreservation of genetic resources is of major interest for the security of biodiversity and for the sustainability of agronomic industry. Cryopreservation of somatic cells, which carry the genome of both parents, is one mean to regenerate functional breeders, but this requires the mastering of nuclear transfer. In mammals, somatic cell nuclear transfer consists in injecting the somatic nuclei into an enucleated mature recipient oocyte, in order to obtain clone which bear the genome of interest from the donor animal. However, in fish species, the oocyte structure makes the enucleation step difficult to achieve (presence of the chorion and bulk nutritive reserve), so a non-enucleated oocyte is often used for nuclear transfer. To obtain a real clone and not hybrid embryos, the oocyte chromatin must therefore be spontaneously excluded from the developing embryo. This phenomenon has been described in several studies but the mechanism involved is still unknown. Deciphering this phenomenon requires a high understanding of the first developmental steps that are meiosis resumption and early mitosis. The aim of the study is to understand the interplay between the maternal chromatin and the injected one upon nuclear transfer during these entire critical steps, in order to identify and use means to improve the success rate of nuclear transfer in fish. Methods: Fin somatic cell and mature oocytes were obtained from 2 years old goldfish (Carassius auratus) females. For nuclear transfer, the whole fin cell was injected under the micropyle. The egg activation was induced 30min after cell injection. Somatic and maternal chromatin during meiosis resumption and early mitosis was characterized by immunofluorescence (hoechst and vybrant labelling for chromatin identification and α-tubulin for spindle organization). Results and Discussion: We observed that a high proportion of clone underwent a normal polar body extrusion after activation (60% vs 96 % for the controls). We hypothesize that in most cases, maternal chromatin achieved correctly its meiosis resumption and that the maternal chromatin behavior was not disturbed by nuclear transfer. However, these observations do not augur early mitosis fitness in clone. Indeed, we observed several spindle defects in clone cells: metaphase misalignment, abnormal segregation, and multicentrosomal spindle, associated with DNA fragmentation. Conclusion: Oocyte non-enucleation for SCNT procedure did not drastically alter the meiosis resumption process, but it could be involved in the perturbation of mitosis during clone early development.
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- 2018
15. Utilisation de l’essai des comètes pour la surveillance des milieux aquatiques – problématique de la conservation des échantillons
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Taïr-Abbaci, K., Labbé, Catherine, Depince, Alexandra, Garnero, L., Bony, Sylvie, Devaux, Alain, Chaumot, Arnaud, Geffard, O., Laboratoire d'Ecotoxicologie, Institut national de recherche en sciences et technologies pour l'environnement et l'agriculture (IRSTEA), Laboratoire de Physiologie et Génomique des Poissons (LPGP), Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique )-Institut National de la Recherche Agronomique (INRA), Laboratoire d'Ecologie des Hydrosystèmes Naturels et Anthropisés (LEHNA), Université Claude Bernard Lyon 1 (UCBL), and Université de Lyon-Université de Lyon-École Nationale des Travaux Publics de l'État (ENTPE)-Centre National de la Recherche Scientifique (CNRS)
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substance chimique ,espèce sentinelle ,bioindicateur ,essai comète ,gammare ,génotoxicité ,encagement ,spermatozoïde ,[SDV]Life Sciences [q-bio] ,test comete ,cryoconservation ,milieu aquatique ,conservation des echantillons ,durée d'exposition ,dégradation de l'adn ,[INFO]Computer Science [cs] ,espèce aquacole ,écotoxicologie - Abstract
National audience; Les milieux aquatiques constituent le réceptacle d’un grand nombre de contaminants, anthropiques et naturels, pouvant être dangereux pour les organismes qui y vivent.
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- 2018
16. Compréhension des perturbations induites par le transfert nucléaire chez le carassin
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Rouillon, Charlène, Depince, Alexandra, Le Bail, Pierre-Yves, Labbé, Catherine, Laboratoire de Physiologie et Génomique des Poissons (LPGP), Institut National de la Recherche Agronomique (INRA)-Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique ), Bourse INRA PHASE-Région Bretagne. Travaux financés pas le Programme Investissements d’Avenir CRB Anim, ANR-11-INBS-0003, and Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique )-Institut National de la Recherche Agronomique (INRA)
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disturbance ,fish ,clone ,nuclear transfer ,méiose ,perturbation ,[SDV]Life Sciences [q-bio] ,adn ,ovocyte ,dna ,cryoconservation ,poisson rouge ,cellule somatique ,transfert nucléaire ,poisson ,cyprinidae ,cryofixation ,meiosis ,somatic cell - Abstract
Compréhension des perturbations induites par le transfert nucléaire chez le carassin. Caractérisation de la reprise de la méiose et des premières mitoses. Journées d'Animation Scientifique du département Phase (JAS Phase 2018)
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- 2018
17. Défauts de reprogrammation épigénétique des embryons issus du transfert nucléaire somatique chez les poissons
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Depince, Alexandra, Le Bail, Pierre-Yves, Labbé, Catherine, Laboratoire de Physiologie et Génomique des Poissons (LPGP), Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique )-Institut National de la Recherche Agronomique (INRA), Institut National de la Recherche Agronomique (INRA)-Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique ), Financé par 'Investissements d’Avenir' ANR-11-INBS-0003 (CRB-Anim 2013-2019) et COST AQUAGAMETE FA 1205, and ProdInra, Archive Ouverte
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fish ,nuclear transfer ,méthylation de l'adn ,[SDV]Life Sciences [q-bio] ,carassius auratus ,adn ,embryo ,dna ,reprogrammation epigénétique ,[SDV] Life Sciences [q-bio] ,poisson rouge ,poisson ,cellule somatique ,transfert nucléaire ,cyprinidae ,embryon ,somatic cell ,goldfish ,méthylation des gènes - Abstract
Défauts de reprogrammation épigénétique des embryons issus du transfert nucléaire somatique chez les poissons. Journées d'Animation Scientifique du département Phase (JAS Phase 2018)
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- 2018
18. Somatic cell nuclear transfer in rainbow trout (Onrohynchus mykiss): What makes it different from fertilization with a spermatozoa ?
- Author
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Depince, Alexandra, Le Bail, Pierre-Yves, Labbé, Catherine, Laboratoire de Physiologie et Génomique des Poissons (LPGP), Institut National de la Recherche Agronomique (INRA)-Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique ), and ANR-11-INBS-0003 (CRB-Anim 2013-2019) - COST AQUAGAMETE FA 1205
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endocrine system ,nuclear transfer ,animal structures ,animal diseases ,[SDV]Life Sciences [q-bio] ,education ,bacterialresistance transfer factor ,physiologie de la reproduction ,reproduction ,fertilité ,poisson ,rainbow ,spermatozoa ,salmonids ,spermatozoïde ,fish ,salmonidae ,trout ,oncorhynchus mykiss ,urogenital system ,cryoconservation ,rainbow trout ,sex factors ,cellule somatique ,transfert nucléaire ,cryofixation ,somatic cell ,truite arc en ciel - Abstract
Somatic cell nuclear transfer in rainbow trout (Onrohynchus mykiss): What makes it different from fertilization with a spermatozoa ?. 11. International Symposium on Reproductive Physiology of Fish
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- 2018
19. Epigenetic reprogramming after nuclear transfer in fish
- Author
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Depince, Alexandra, Le Bail, Pierre-Yves, Labbé, Catherine, Laboratoire de Physiologie et Génomique des Poissons (LPGP), Institut National de la Recherche Agronomique (INRA)-Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique ), Financial support of 'Investissements d’Avenir' ANR-11-INBS-0003 (CRB-Anim 2013–2019) and COST AQUAGAMETE FA 1205, University of South Bohemia in České Budějovice. CZE., and ProdInra, Archive Ouverte
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fish ,nuclear transfer ,DNA methylation ,méthylation de l'adn ,health care facilities, manpower, and services ,[SDV]Life Sciences [q-bio] ,education ,epigenetic reprogramming ,reprogrammation epigénétique ,cryoconservation ,somatic cell nuclear transfer ,[SDV] Life Sciences [q-bio] ,poisson rouge ,reproduction ,oeuf de poisson ,poisson ,cellule somatique ,transfert nucléaire ,cyprinidae ,cryofixation ,somatic cell ,goldfish ,ComputingMilieux_MISCELLANEOUS ,health care economics and organizations ,expression des gènes - Abstract
Epigenetic reprogramming after nuclear transfer in fish. 6. International Workshop on the Biology of Fish Gametes
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- 2017
20. Meiosis resumption and early mitosis pattern after somatic cell nuclear transfer in goldfish
- Author
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Rouillon, Charlène, Depince, Alexandra, Le Bail, Pierre-Yves, Labbé, Catherine, Laboratoire de Physiologie et Génomique des Poissons (LPGP), Institut National de la Recherche Agronomique (INRA)-Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique ), This work is funded by the French CRB Anim project «Investissements d’avenir», ANR- 11-INBS-0003. CR is recipient of an INRA-PHASE and Région Bretagne fellowship., University of South Bohemia in České Budějovice. CZE., ANR-11-INBS-0003,CRB-Anim,Réseau de Centres de Ressources Biologiques pour les animaux domestiques(2011), ProdInra, Archive Ouverte, and Infrastructures - Réseau de Centres de Ressources Biologiques pour les animaux domestiques - - CRB-Anim2011 - ANR-11-INBS-0003 - INBS - VALID
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nuclear transfer ,[SDV]Life Sciences [q-bio] ,education ,embryo ,régénération cellulaire ,reproduction ,poisson ,cyprinidae ,early mitosis ,foetal development ,fish ,conservation ,reprogramming ,transition mitose meiose ,cryoconservation ,mortality ,[SDV] Life Sciences [q-bio] ,poisson rouge ,cellule somatique ,transfert nucléaire ,développement embryonnaire ,embryon ,cryofixation ,somatic cell ,sense organs ,conservation des ressources génétiques ,mortalité - Abstract
Meiosis resumption and early mitosis pattern after somatic cell nuclear transfer in goldfish. 6. International Workshop on the Biology of Fish Gametes
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- 2017
21. Epigenetic stability in goldfish sperm upon hormonal induction and over the breeding season
- Author
-
Depince, Alexandra, Bertin, Chloé, Labbé, Catherine, Laboratoire de Physiologie et Génomique des Poissons (LPGP), Institut National de la Recherche Agronomique (INRA)-Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique ), This work is funded by the French CRB Anim project «Investissements d’avenir», ANR-11-INBS-0003, ProdInra, Archive Ouverte, and University of South Bohemia in České Budějovice. CZE.
- Subjects
fish ,DNA methylation ,sperm quality ,urogenital system ,méthylation de l'adn ,[SDV]Life Sciences [q-bio] ,education ,spermatogenèse ,qualité du sperme ,spermatogenesis ,[SDV] Life Sciences [q-bio] ,poisson rouge ,reproduction ,épigénétique ,nervous system ,poisson ,spermatozoa ,biotechnologie de la reproduction ,cyprinidae ,chromatin ,spermatozoïde ,sense organs ,induction hormonale ,chromatine ,health care economics and organizations - Abstract
Epigenetic stability in goldfish sperm upon hormonal induction and over the breeding season. 6. International Workshop on the Biology of Fish Gametes
- Published
- 2017
22. Single and multiple somatic cells nuclear transfer in critically endangered species, sturgeon
- Author
-
FATIRA, Effrosiny, Labbé, Catherine, Depince, Alexandra, Legorova, Victoriia, Pocherniaieva, Kseniia, Güralp, Hilal, Havelka, Miloš, Pšenička, Martin, Saito, Taiju, Faculty of Fisheries and Protection of Waters, Laboratory of Germ Cells, University of South Bohemia, Laboratoire de Physiologie et Génomique des Poissons (LPGP), Institut National de la Recherche Agronomique (INRA)-Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique ), Faculty of Fisheries Sciences [Hakodate], Hokkaido University [Sapporo, Japan], South Ehime Fisheries Research Center, Ehime University [Matsuyama], The study was supported from Grant Agency of the University of South Bohemia in České Budějovice, GAJU Fatira 068/2017/Z 2017., University of South Bohemia in České Budějovice. CZE., Hokkaido University, and ProdInra, Archive Ouverte
- Subjects
fish ,acipenseridae ,nuclear transfer ,[SDV]Life Sciences [q-bio] ,education ,cloning ,espèce en danger ,mise au point de technique ,sturgeon ,intraspecies SCNT ,chimera ,esturgeon commun ,[SDV] Life Sciences [q-bio] ,reproduction ,poisson ,cellule somatique ,transfert nucléaire ,species threatened with extinction ,clonage ,somatic cell ,transplantation cellulaire ,single and multi fins micro injection ,interspecies SCNT - Abstract
Single and multiple somatic cells nuclear transfer in critically endangered species, sturgeon. 6. International Workshop on the Biology of Fish Gametes
- Published
- 2017
23. Optimisation des conditions d’élevage des poissons rouges : obtention de gamètes et réduction du nombre d’animaux expérimentaux
- Author
-
Depince, Alexandra, Laboratoire de Physiologie et Génomique des Poissons (LPGP), Institut National de la Recherche Agronomique (INRA)-Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique ), and ProdInra, Archive Ouverte
- Subjects
[SDV] Life Sciences [q-bio] ,fish ,poisson rouge ,condition d'élevage ,poisson ,cyprinidae ,[SDV]Life Sciences [q-bio] ,expérimentation - Abstract
Optimisation des conditions d’élevage des poissons rouges : obtention de gamètes et réduction du nombre d’animaux expérimentaux. 2. Journées des Installations Expérimentales LPGP
- Published
- 2017
24. Epigenetic and cellular reprogramming after nuclear transfer in fish : characterization of clone defects in relation to the donor cell origin
- Author
-
Rouillon, Charlène, Depince, Alexandra, Le Bail, Pierre-Yves, Labbé, Catherine, Laboratoire de Physiologie et Génomique des Poissons (LPGP), Institut National de la Recherche Agronomique (INRA)-Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique ), Centre National de la Recherche Scientifique (CNRS). FRA., and ProdInra, Archive Ouverte
- Subjects
fish ,nuclear transfer ,[SDV]Life Sciences [q-bio] ,conservation ,reprogramming ,reprogrammation epigénétique ,cryoconservation ,[SDV] Life Sciences [q-bio] ,épigénétique ,poisson ,cellule somatique ,transfert nucléaire ,cryofixation ,somatic cell ,epigenetic - Abstract
Epigenetic and cellular reprogramming after nuclear transfer in fish : characterization of clone defects in relation to the donor cell origin. 2. Journées scientifiques GDR 3606 REPRO : ReproSciences 2017
- Published
- 2017
25. Cryoconservation de la semence de poisson : importance du maintien de l'intégrité épigénétique
- Author
-
Depince, Alexandra, Labbé, Catherine, Laboratoire de Physiologie et Génomique des Poissons (LPGP), Institut National de la Recherche Agronomique (INRA)-Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique ), ANR-11-INBS-003 5 CRB-Anim 2013-2019 - COST AQUAGAMETE FA 1205, Institut Technique de l'Aviculture et des Elevages de Petits Animaux (ITAVI). FRA., and ProdInra, Migration
- Subjects
[SDV] Life Sciences [q-bio] ,[SDV]Life Sciences [q-bio] ,[INFO]Computer Science [cs] ,[INFO] Computer Science [cs] ,ComputingMilieux_MISCELLANEOUS - Abstract
International audience
- Published
- 2016
26. Stemness properties and molecular signature of paired spermatogonia in adult zebrafish
- Author
-
Curran, Edouard, Gautier, Aude, Saito, Taiju, Patinote, Amélie, Depince, Alexandra, Labbé, Catherine, Chalmel, Frédéric, Le Gac, Florence, Lareyre, Jean-Jacques, Laboratoire de Physiologie et Génomique des Poissons (LPGP), Institut National de la Recherche Agronomique (INRA)-Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique ), University of South Bohemia, Institut National de la Santé et de la Recherche Médicale (INSERM), ProdInra, Migration, Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique )-Institut National de la Recherche Agronomique (INRA), Institut de recherche en santé, environnement et travail (Irset), Université d'Angers (UA)-Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES)-École des Hautes Études en Santé Publique [EHESP] (EHESP)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique ), and Université d'Angers (UA)-Université de Rennes (UR)-École des Hautes Études en Santé Publique [EHESP] (EHESP)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique )
- Subjects
[SDV] Life Sciences [q-bio] ,[SDV]Life Sciences [q-bio] ,[INFO]Computer Science [cs] ,[INFO] Computer Science [cs] ,ComputingMilieux_MISCELLANEOUS - Abstract
International audience
- Published
- 2016
27. Isolement et caractérisation de cellules germinales souches adultes à partir de lignées de poissons zèbre transgéniques
- Author
-
Curran, Edouard, Gautier, Aude, Saito, Taiju, Patinote, Amélie, Depince, Alexandra, Labbé, Catherine, Pain, Bertrand, Chalmel, Frédéric, Le Gac, Florence, Lareyre, Jean-Jacques, ProdInra, Migration, Laboratoire de Physiologie et Génomique des Poissons (LPGP), Institut National de la Recherche Agronomique (INRA)-Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique ), University of Bohemia, Partenaires INRAE, Institut cellule souche et cerveau (U846 Inserm - UCBL1), Institut National de la Recherche Agronomique (INRA)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut National de la Santé et de la Recherche Médicale (INSERM), and Institut National de la Santé et de la Recherche Médicale (INSERM)
- Subjects
[SDV] Life Sciences [q-bio] ,[SDV]Life Sciences [q-bio] ,[INFO]Computer Science [cs] ,[INFO] Computer Science [cs] ,cellule germinale souche ,régénération ,transcriptome ,ComputingMilieux_MISCELLANEOUS ,transplantation - Abstract
International audience
- Published
- 2016
28. Les cellules somatiques : un intérêt pour la conservation des ressources génétiques chez les poissons
- Author
-
Chenais, Nathalie, Depince, Alexandra, Le Bail, Pierre-Yves, Labbé, Catherine, ProdInra, Migration, Laboratoire de Physiologie et Génomique des Poissons (LPGP), Institut National de la Recherche Agronomique (INRA)-Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique ), Société Fançaise d'Ichtyologie. FRA., and Institut Technique de l'Aviculture et des Elevages de Petits Animaux (ITAVI). FRA.
- Subjects
cellules nageoire ,[SDV]Life Sciences [q-bio] ,biotechnologie animale ,[INFO] Computer Science [cs] ,cryoconservation ,ressource génétique ,[SDV] Life Sciences [q-bio] ,nageoire ,information génétique ,poisson ,cellule somatique ,transfert nucléaire ,biotechnologie de la reproduction ,culture primaire ,[INFO]Computer Science [cs] ,reprogrammation ,ComputingMilieux_MISCELLANEOUS ,expression des gènes - Abstract
International audience
- Published
- 2016
29. Reprogrammation épigénétique après transfert nucléaire chez les poissons
- Author
-
Depince, Alexandra, Le Bail, Pierre-Yves, Labbé, Catherine, ProdInra, Migration, Laboratoire de Physiologie et Génomique des Poissons (LPGP), Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique )-Institut National de la Recherche Agronomique (INRA), and Institut National de Recherche Agronomique (INRA). UAR Département Physiologie Animale et Systèmes d'Elevage (0558).
- Subjects
[SDV] Life Sciences [q-bio] ,[SDV]Life Sciences [q-bio] ,[INFO]Computer Science [cs] ,[INFO] Computer Science [cs] ,ComputingMilieux_MISCELLANEOUS - Abstract
National audience
- Published
- 2016
30. Reprogramming defects after nuclear transfer in fish
- Author
-
Depince, Alexandra, Le Bail, Pierre-Yves, Chenais, Nathalie, Jammes, Hélène, Labbé, Catherine, Laboratoire de Physiologie et Génomique des Poissons (LPGP), Institut National de la Recherche Agronomique (INRA)-Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique ), Biologie du Développement et Reproduction (BDR), École nationale vétérinaire d'Alfort (ENVA)-Institut National de la Recherche Agronomique (INRA), ANR-11-INBS-0003 (CRB-Anim 2013-2019) - COST Aquagamete FA1205, and European Cooperation in Science and Technology (COST). BEL.
- Subjects
[SDV]Life Sciences [q-bio] ,[INFO]Computer Science [cs] - Abstract
International audience; Somatic cells bear a major potential as cell resource for biodiversity conservation: they carry the genome of both parents, and they can be collected whatever the age or sex of the donor. In fish, cryobanking of somatic cells is highly valuable because eggs and embryos cannot be cryopreserved. Then, the sole use of cryopreserved sperm for animal restoration requires long back-crossing with fresh eggs from plain strains. With somatic cells, the regeneration of the donor genotype will however rely on the nuclear transfer technology in which the donor cell nucleus is injected into a recipient oocyte. Although this restoration technology was developed in the 60’ for fish, the method is still far from being reliable enough for genome preservation and population restoration. Using the goldfish as a model, we showed that the most critical stage after nuclear transfer was the mid-blastula transition stage, when the 1000 cells embryo triggers its own genome activation. This strongly suggests that although the injected nucleus faced 10 rounds of mitosis, chromatin reprogramming was not reliable enough to allow the proper expression of the early development genes. We demonstrated that several marker genes whose promoter regions are hypomethylated in fertilized early embryos displayed various patterns of hypermethylation which were closer to the donor cell pattern. To some extent, the morphology of the embryo could be correlated to the level of the DNA methylation reprogramming. This demonstration reinforces the need to reprogram the donor cell prior to or during nuclear transfer, and the best strategies when facing fish cells and embryos will be discussed.
- Published
- 2015
31. Development of cryopreservation of zebrafish (Danio rerio) sperm
- Author
-
Kollar, Timea, Horvath, Akos, Labesse, Charlène, Milon, Pierre, Csenki, Zsolt, Kovács, Róbert, Bernáth, Gergely, Béres, Tibor, Depince, Alexandra, Urbányi, Béla, Labbé, Catherine, Department of Aquaculture, Szent István University, Laboratoire de Physiologie et Génomique des Poissons (LPGP), Institut National de la Recherche Agronomique (INRA)-Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique ), COST Action FA 1205 AQUAGAMETE, Università Politecnica delle Marche. ITA., and ProdInra, Migration
- Subjects
[SDV] Life Sciences [q-bio] ,[SDV]Life Sciences [q-bio] ,[INFO]Computer Science [cs] ,[INFO] Computer Science [cs] ,ComputingMilieux_MISCELLANEOUS - Abstract
International audience
- Published
- 2015
32. Cryoprotectants alter DNA methylation pattern of spermatozoa and decrease reproductive success in Colossoma macropomum
- Author
-
de Mello, Fernanda, Garcia, Juliana Saraiva, de Godoy, Leandro C., Depince, Alexandra, Labbé, Catherine, Streit, Danilo P. Jr., Laboratory of Aquaculture, Universidade Federal do Rio Grande do Sul [Porto Alegre] (UFRGS), Federal University of Rio Grande do Sul, Laboratory of Biotechnology Applied to Fish Reproduction, Nilton Lins University, Laboratoire de Physiologie et Génomique des Poissons (LPGP), Institut National de la Recherche Agronomique (INRA)-Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique ), COST Action FA 1205 AQUAGAMETE, and Università Politecnica delle Marche. ITA.
- Subjects
[SDV]Life Sciences [q-bio] ,[INFO]Computer Science [cs] ,ComputingMilieux_MISCELLANEOUS - Abstract
International audience
- Published
- 2015
33. Molecular signature and stemness properties of paired spermatogonia in zebrafish
- Author
-
Curran, Edouard, Gautier, Aude, Saito, Taiju, Patinote, Amélie, Depince, Alexandra, Labbé, Catherine, Le Gac, Florence, Lareyre, Jean-Jacques, Laboratoire de Physiologie et Génomique des Poissons (LPGP), Institut National de la Recherche Agronomique (INRA)-Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique ), University of South Bohemia, COST Action FA 1205 AQUAGAMETE, Università Politecnica delle Marche. ITA., and ProdInra, Migration
- Subjects
[SDV] Life Sciences [q-bio] ,[SDV]Life Sciences [q-bio] ,[INFO]Computer Science [cs] ,[INFO] Computer Science [cs] ,ComputingMilieux_MISCELLANEOUS - Abstract
International audience
- Published
- 2015
34. Impact de la cryoconservation du sperme sur la méthylation de l'ADN : l'effet espèce chez les poissons
- Author
-
Labbé, Catherine, Depince, Alexandra, Millon, Pierre, Gabory, Anne, Jammes, Hélène, Laboratoire de Physiologie et Génomique des Poissons (LPGP), Institut National de la Recherche Agronomique (INRA)-Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique ), Biologie du Développement et Reproduction (BDR), École nationale vétérinaire d'Alfort (ENVA)-Institut National de la Recherche Agronomique (INRA), and Institut National de Recherche Agronomique (INRA). UAR Département Physiologie Animale et Systèmes d'Elevage (0558).
- Subjects
[SDV]Life Sciences [q-bio] ,[INFO]Computer Science [cs] ,ComputingMilieux_MISCELLANEOUS - Abstract
National audience
- Published
- 2015
35. Clonage et transplantation de cellules germinales, pour la conservation des ressources génétiques
- Author
-
Depince, Alexandra, ProdInra, Migration, Laboratoire de Physiologie et Génomique des Poissons (LPGP), and Institut National de la Recherche Agronomique (INRA)-Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique )
- Subjects
[SDV] Life Sciences [q-bio] ,[SDV]Life Sciences [q-bio] ,[INFO]Computer Science [cs] ,[INFO] Computer Science [cs] ,ComputingMilieux_MISCELLANEOUS - Abstract
National audience
- Published
- 2015
36. Méthylation globale de la semence de poisson rouge après cryoconservation. Effet des cryoprotecteurs
- Author
-
Depince, Alexandra, Labbé, Catherine, Laboratoire de Physiologie et Génomique des Poissons (LPGP), Institut National de la Recherche Agronomique (INRA)-Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique ), and ProdInra, Migration
- Subjects
[SDV.OT]Life Sciences [q-bio]/Other [q-bio.OT] ,[SDV.OT] Life Sciences [q-bio]/Other [q-bio.OT] ,ComputingMilieux_MISCELLANEOUS - Abstract
National audience
- Published
- 2014
37. Cryopreserving European eel (A. anguilla) sperm: comparison of two methods for standardization
- Author
-
Vilchez, Maria C., Morini, Marina, Penaranda, David S., Pérez, Luz, Depince, Alexandra, Kasa, Esztzer, Labbé, Catherine, Horvath, Akos, Asturiano, Juan F., Universitat Politècnica de València (UPV), Instituto de Ciencia y Tecnologia Animal, Partenaires INRAE, Laboratoire de Physiologie et Génomique des Poissons (LPGP), Institut National de la Recherche Agronomique (INRA)-Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique ), Szent István University, CRB-Anim project 'Investissements d'Avenir 'ANR-11-INBS-0003 & COST Action FA1205: AQUAGAMETE, European Project: 245257,EC:FP7:KBBE,FP7-KBBE-2009-3,PRO-EEL(2010), and Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique )-Institut National de la Recherche Agronomique (INRA)
- Subjects
poisson ,cryoconservation du sperme ,méthylation de l'adn ,anguille ,[SDV]Life Sciences [q-bio] ,anguilla anguilla ,méthode de conservation ,[INFO]Computer Science [cs] ,cryoconservation ,ComputingMilieux_MISCELLANEOUS - Abstract
International audience
- Published
- 2014
38. Dna methylation of fish germ cells and the risk of alteration after cryopreservation
- Author
-
Labbé, Catherine, Depince, Alexandra, Milon, Pierre, Morini, M., Riesco, M., Robles, V., Asturiano, J., Horvath, A., Herraez, P., Laboratoire de Physiologie et Génomique des Poissons (LPGP), Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique )-Institut National de la Recherche Agronomique (INRA), Physiologie de la reproduction et des comportements [Nouzilly] (PRC), Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours (UT)-Centre National de la Recherche Scientifique (CNRS), Institute of Animal Science and Technology, Universitat Politècnica de València (UPV), Universidad de León [León], Department of Aquaculture, Szent István University, Department of Molecular Biology, University of Leon, CRB-Anim project 'Investissements d'Avenir' ANR-11-INBS-0003, Center of Marine Sciences (CCMAR). PRT., Institut National de la Recherche Agronomique (INRA)-Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique ), Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours-Centre National de la Recherche Scientifique (CNRS), and ProdInra, Migration
- Subjects
[SDV.OT]Life Sciences [q-bio]/Other [q-bio.OT] ,[SDV.OT] Life Sciences [q-bio]/Other [q-bio.OT] ,ComputingMilieux_MISCELLANEOUS - Abstract
International audience
- Published
- 2014
39. DNA methylation pattern in fish spermatozoa after cryopreservation: a species specific alteration in cyprinids
- Author
-
Labbé, Catherine, Depince, Alexandra, Milon, Pierre, Jammes, Hélène, Gabory, Anne, Laboratoire de Physiologie et Génomique des Poissons (LPGP), Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique )-Institut National de la Recherche Agronomique (INRA), Physiologie de la reproduction et des comportements [Nouzilly] (PRC), Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours (UT)-Centre National de la Recherche Scientifique (CNRS), Biologie du développement et reproduction (BDR), Centre National de la Recherche Scientifique (CNRS)-École nationale vétérinaire d'Alfort (ENVA)-Institut National de la Recherche Agronomique (INRA), ANR-11-INBS-0003, European Cooperation in Science and Technology (COST). BEL., Institut National de la Recherche Agronomique (INRA)-Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique ), Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours-Centre National de la Recherche Scientifique (CNRS), École nationale vétérinaire d'Alfort (ENVA)-Institut National de la Recherche Agronomique (INRA)-Centre National de la Recherche Scientifique (CNRS), and ProdInra, Migration
- Subjects
[SDV.OT]Life Sciences [q-bio]/Other [q-bio.OT] ,[SDV.OT] Life Sciences [q-bio]/Other [q-bio.OT] ,ComputingMilieux_MISCELLANEOUS - Abstract
International audience
- Published
- 2014
40. DNA methylation pattern after cryopreservation of goldfish cells: influence of the type of cryoprotectant
- Author
-
Depince, Alexandra, Jammes, Hélène, Gabory, Anne, Labbé, Catherine, Laboratoire de Physiologie et Génomique des Poissons (LPGP), Institut National de la Recherche Agronomique (INRA)-Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique ), Biologie du développement et reproduction (BDR), École nationale vétérinaire d'Alfort (ENVA)-Institut National de la Recherche Agronomique (INRA)-Centre National de la Recherche Scientifique (CNRS), ANR-11-INBS-0003, and European Cooperation in Science and Technology (COST). BEL.
- Subjects
[SDV.OT]Life Sciences [q-bio]/Other [q-bio.OT] ,ComputingMilieux_MISCELLANEOUS - Abstract
International audience
- Published
- 2014
41. Insight into the DNA methylation of fish germ cells, into the risk of alteration after cryopreservation, and possible consequences on embryo development
- Author
-
Labbé, Catherine, Robles, Vanesa, Depince, Alexandra, Riesco, Martin, F., Milon, Pierre, Conradt, Marie, Le Bail, Pierre-Yves, Herraez, Paz, Laboratoire de Physiologie et Génomique des Poissons (LPGP), Institut National de la Recherche Agronomique (INRA)-Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique ), Universidad de León [León], and ProdInra, Migration
- Subjects
[SDV.OT]Life Sciences [q-bio]/Other [q-bio.OT] ,[SDV.OT] Life Sciences [q-bio]/Other [q-bio.OT] ,ComputingMilieux_MISCELLANEOUS - Abstract
International audience
- Published
- 2013
42. Changes in sperm DNA methylation after cryopreservation in fish
- Author
-
Labbé, Catherine, Conradt, Marie, Depince, Alexandra, Gabory, Anne, Jammes, Helene, Le Bail, Pierre-Yves, Station commune de Recherches en Ichtyophysiologie, Biodiversité et Environnement (SCRIBE), Institut National de la Recherche Agronomique (INRA)-IFR140, Biologie du Développement et Reproduction (BDR), Institut National de la Recherche Agronomique (INRA), Investissements d'Avenir, ANR-11-INBS-0003, Laboratoire de Physiologie et Génomique des Poissons (LPGP), Institut National de la Recherche Agronomique (INRA)-Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique ), Biologie du développement et reproduction (BDR), and École nationale vétérinaire d'Alfort (ENVA)-Institut National de la Recherche Agronomique (INRA)-Centre National de la Recherche Scientifique (CNRS)
- Subjects
[SDV.BA]Life Sciences [q-bio]/Animal biology ,[SDV.BDLR]Life Sciences [q-bio]/Reproductive Biology - Abstract
absent
- Published
- 2013
43. Rôle de la TSA dans le maintien de la dé‐différentiation des cellules embryonnaires isolées chez le poisson rouge
- Author
-
Le Bail, Pierre-Yves, Chenais, Nathalie, Depince, Alexandra, Labbé, Catherine, ProdInra, Migration, Laboratoire de Physiologie et Génomique des Poissons (LPGP), Institut National de la Recherche Agronomique (INRA)-Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique ), and Institut National de Recherche Agronomique (INRA). UAR Département Physiologie Animale et Systèmes d'Elevage (0558).
- Subjects
[SDV] Life Sciences [q-bio] ,[SDV]Life Sciences [q-bio] ,[INFO]Computer Science [cs] ,[INFO] Computer Science [cs] ,ComputingMilieux_MISCELLANEOUS - Abstract
National audience
- Published
- 2013
44. Le transfert nucléaire chez les poissons : promesses et difficultés pour la régénération d'un patrimoine génétique diploïde cryoconservé
- Author
-
Labbé, Catherine, Depince, Alexandra, Chenais, Nathalie, Marandel, Lucie, Mella Alvarado, Vanessa, Gautier, Laurent, Le Bail, Pierre-Yves, Laboratoire de Physiologie et Génomique des Poissons (LPGP), Institut National de la Recherche Agronomique (INRA)-Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique ), and ProdInra, Migration
- Subjects
[SDV.BIO]Life Sciences [q-bio]/Biotechnology ,ComputingMilieux_MISCELLANEOUS ,[SDV.BIO] Life Sciences [q-bio]/Biotechnology - Abstract
National audience
- Published
- 2012
45. Goldfish embryo development after somatic cell nuclear transfer in non-enucleated oocytes: first mitosis profile, ploidy status, and genetic conformity
- Author
-
Labbé, Catherine, Depince, Alexandra, Chenais, Nathalie, Marandel, Lucie, Le Bail, Pierre-Yves, Station commune de Recherches en Ichtyophysiologie, Biodiversité et Environnement (SCRIBE), Institut National de la Recherche Agronomique (INRA), Institut Fédératif de Recherche - Génétique Fonctionnelle Agronomie et Santé (IFR 140 GFAS), Plateforme Génomique Santé Biogenouest®, and ProdInra, Migration
- Subjects
[SDV] Life Sciences [q-bio] ,[SDV]Life Sciences [q-bio] ,[INFO]Computer Science [cs] ,[INFO] Computer Science [cs] ,ComputingMilieux_MISCELLANEOUS - Abstract
International audience
- Published
- 2011
46. Nuclear transfer with cryopreserved fin cells, a unique but difficult means to reconstruct fish with diploid cells
- Author
-
Labbé, Catherine, Le Bail, Pierre-Yves, Depince, Alexandra, Mella, Vanessa, Chenais, Nathalie, ProdInra, Migration, Station commune de Recherches en Ichtyophysiologie, Biodiversité et Environnement (SCRIBE), Institut National de la Recherche Agronomique (INRA), Institut Fédératif de Recherche - Génétique Fonctionnelle Agronomie et Santé (IFR 140 GFAS), and Plateforme Génomique Santé Biogenouest®
- Subjects
[SDV] Life Sciences [q-bio] ,epigenetics ,aquatic species ,[SDV]Life Sciences [q-bio] ,cryobanking ,microinjection ,reprogramming ,[INFO]Computer Science [cs] ,embryo development ,GENETIQUE ,[INFO] Computer Science [cs] ,ComputingMilieux_MISCELLANEOUS ,biotechnology - Abstract
International audience
- Published
- 2011
47. DNA methylation pattern in sperm and somatic cells after cryopreservation: Influence of the cryoprotectant
- Author
-
Gautier, Laurent, Depince, Alexandra, Chenais, Nathalie, Marandel, Lucie, Le Bail, Pierre-Yves, Labbé, Catherine, ProdInra, Migration, Station commune de Recherches en Ichtyophysiologie, Biodiversité et Environnement (SCRIBE), Institut National de la Recherche Agronomique (INRA), Institut Fédératif de Recherche - Génétique Fonctionnelle Agronomie et Santé (IFR 140 GFAS), and Plateforme Génomique Santé Biogenouest®
- Subjects
[SDV] Life Sciences [q-bio] ,[SDV]Life Sciences [q-bio] ,[INFO]Computer Science [cs] ,[INFO] Computer Science [cs] ,ComputingMilieux_MISCELLANEOUS - Abstract
International audience
- Published
- 2011
48. Obtention de cellules de nageoire en culture à partir de fragments de nageoire cryoconservés chez le carassin : une stratégie fiable de conservation du génome
- Author
-
Moritz, Charlotte, Depince, Alexandra, Labbé, Catherine, Station commune de Recherches en Ichtyophysiologie, Biodiversité et Environnement (SCRIBE), Institut National de la Recherche Agronomique (INRA), and ProdInra, Migration
- Subjects
[SDV] Life Sciences [q-bio] ,[SDV]Life Sciences [q-bio] ,[INFO]Computer Science [cs] ,[INFO] Computer Science [cs] ,CARASSIUS AURATUS ,ComputingMilieux_MISCELLANEOUS - Abstract
National audience
- Published
- 2007
49. Optimisation des conditions de manipulation des ovules en vue du transfert nucléaire chez le carassin
- Author
-
Chenais, Nathalie, Mahé, Sophie, Vignon, Xavier, Depince, Alexandra, Bobe, Julien, Le Bail, Pierre-Yves, Labbé, Catherine, Station commune de Recherches en Ichtyophysiologie, Biodiversité et Environnement (SCRIBE), Institut National de la Recherche Agronomique (INRA), Biologie du développement et reproduction (BDR), and Centre National de la Recherche Scientifique (CNRS)-École nationale vétérinaire d'Alfort (ENVA)-Institut National de la Recherche Agronomique (INRA)
- Subjects
[SDV]Life Sciences [q-bio] ,[INFO]Computer Science [cs] ,CARASSIUS AURATUS ,ComputingMilieux_MISCELLANEOUS - Abstract
National audience
- Published
- 2007
50. A preliminary approach for nuclear transfer in goldfish: reduction in the variability of egg quality, optimization of egg manipulation
- Author
-
Chenais, Nathalie, Mahé, Sophie, Vignon, Xavier, Depince, Alexandra, Bobe, Julien, Le Bail, Pierre-Yves, Labbé, Catherine, Station commune de Recherches en Ichtyophysiologie, Biodiversité et Environnement (SCRIBE), Institut National de la Recherche Agronomique (INRA), Biologie du développement et reproduction (BDR), and Centre National de la Recherche Scientifique (CNRS)-École nationale vétérinaire d'Alfort (ENVA)-Institut National de la Recherche Agronomique (INRA)
- Subjects
late blastula ,fertilization ,embryonic structures ,[SDV.BDLR]Life Sciences [q-bio]/Reproductive Biology ,egg storage ,development ,[SDV.BDD]Life Sciences [q-bio]/Development Biology - Abstract
National audience; The development of nuclear transfer in Goldfish (Carassius auratus) requires egg handling to be optimized 1) to maintain the ability of the egg to withstand embryo development 2) to allow micromanipulation in order to enucleate the egg and inject the donor nucleus 3) to favour egg cytoplasm ability for nucleus reprogramming. In this work, several egg-handling conditions were tested and optimized in the context of nuclear transfer experiments. To obtain the best egg quality, eggs should be left in the female and stripped out just before use, as their ability to withstand embryo development decreased after in vitro storage at 4 or 20 degrees C. Egg enucleation required that the chorion be removed by enzymatic digestion, and a fast method is described which allowed chorion removal within 1 min post activation. After activation, polar body extrusion should be used as an indicator of the pronucleus position underneath, due to failure to label egg DNA. The enucleation success remained low. In experiments where the enucleation proccess was not carried out, chorion removal was therefore not necessary. In this case, egg incubation conditions preventing egg activation were developed in order to improve nucleus reprogramming after injection of eggs with high MPF levels. Trout coelomic fluid proved to be the best inactivation medium when compared to the proteasome inhibitor MG132 and to the soybean trypsin inhibitor. In our experimental conditions, clones were obtained with embryonic and somatic nuclei, but none developed further than the onset of zygotic transcription (1000 cells stage).
- Published
- 2007
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