Search

Your search keyword '"Depince, Alexandra"' showing total 68 results
Start Over Author "Depince, Alexandra"
68 results on '"Depince, Alexandra"'

4. Characterization of a new embryonic cellular model for nuclear transfer in fish: the embryoid body

Author

Rouillon, Charlène, Depince, Alexandra, Le Bail, Pierre-Yves, Labbé, Catherine, Laboratoire de Physiologie et Génomique des Poissons (LPGP), Institut National de la Recherche Agronomique (INRA)-Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique ), CR is recipient of an INRA-PHASE and Région Bretagne fellowship. This work is funded by the French CRB Anim project «Investissements d’avenir», ANR-11-INBS-0003, ANR-11-INBS-0003,CRB-Anim,Réseau de Centres de Ressources Biologiques pour les animaux domestiques(2011), Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique )-Institut National de la Recherche Agronomique (INRA), ProdInra, Archive Ouverte, and Infrastructures - Réseau de Centres de Ressources Biologiques pour les animaux domestiques - - CRB-Anim2011 - ANR-11-INBS-0003 - INBS - VALID

Subjects
[SDV] Life Sciences [q-bio], reprograming, DNA methylation, [SDV]Life Sciences [q-bio], embryonic structures, education, carassius auratus, trichostatin A, transcription
Abstract

Characterization of a new embryonic cellular model for nuclear transfer in fish: the embryoid body. 7. International Workshop on the Biology of Fish Gametes

Published
2019

5. Comparative analysis of global DNA methylation in spermatozoa from fish, molluscs, bird and mammals: effect of cryopreservation

Author

Depince, Alexandra, Jammes, Helene, Labbé, Catherine, Laboratoire de Physiologie et Génomique des Poissons (LPGP), Institut National de la Recherche Agronomique (INRA)-Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique ), Biologie du Développement et Reproduction (BDR), École nationale vétérinaire d'Alfort (ENVA)-Institut National de la Recherche Agronomique (INRA), Funding support of 'Investissements d’Avenir' ANR-11-INBS-0003 (CRB-Anim 2013-2019), ANR-11-INBS-0003,CRB-Anim,Réseau de Centres de Ressources Biologiques pour les animaux domestiques(2011), Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique )-Institut National de la Recherche Agronomique (INRA), Biologie du développement et reproduction (BDR), École nationale vétérinaire d'Alfort (ENVA)-Institut National de la Recherche Agronomique (INRA)-Centre National de la Recherche Scientifique (CNRS), and Funding support of 'Investissements d’Avenir'ANR-11-INBS-0003 (CRB-Anim 2013-2019)

Subjects
urogenital system, [SDV]Life Sciences [q-bio], education, gamete, reproductive and urinary physiology, epigenetic, biotechnology
Abstract

Comparative analysis of global DNA methylation in spermatozoa from fish, molluscs, bird and mammals: effect of cryopreservation. 7. International Workshop on the Biology of Fish Gametes

Published
2019

6. Changes in sperm cryopreservation procedure for industrial needs

Author

Labbé, Catherine, Delhomme, Guy, Depince, Alexandra, Gavin-Plagne, Lucie, Goardon, Lionel, Kica, Stéphanie, Leboucher, Richard, Morvezen, Romain, Na, Li, Laboratoire de Physiologie et Génomique des Poissons (LPGP), Institut National de la Recherche Agronomique (INRA)-Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique ), IMV Technologies, Pisciculture Expérimentale INRA des Monts d'Arrée (PEIMA), Institut National de la Recherche Agronomique (INRA), Syndicat des Sélectionneurs Avicoles et Aquacoles Français (SYSAAF), Work funded by the FEAMP BIOGERM project and by the PIA CRB Anim (ANR-11-INBS-0003), ANR-11-INBS-0003,CRB-Anim,Réseau de Centres de Ressources Biologiques pour les animaux domestiques(2011), Syndicat des Sélectionneurs Avicoles et Aquacoles Français, and Work funded by the FEAMP BIOGERM (2017, mesure 49) project and by the PIA CRB Anim (ANR-11-INBS-0003

Subjects
Cryoconservation, raft, urogenital system, [SDV]Life Sciences [q-bio], education, MeOH, Poisson, Digitcool, Rainbow trout, Cryoconservation du sperme, neomale, Oncorhynchus mykiss, Sperme, sense organs, skin and connective tissue diseases, DMSO, health care economics and organizations
Abstract

Changes in sperm cryopreservation procedure for industrial needs. 7. International Workshop on the Biology of Fish Gametes

Published
2019

7. Cryoconservation du sperme de salmonidés : évolution des pratiques et amélioration des fécondances

Author

Labbé, Catherine, Delhomme, Guy, Depince, Alexandra, Gavin-Plagne, Lucie, Goardon, Lionel, Kica, Stéphanie, Leboucher, Richard, Morvezen, Romain, Li, Na, Laboratoire de Physiologie et Génomique des Poissons (LPGP), Institut National de la Recherche Agronomique (INRA)-Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique ), IMV-Technologies, Pisciculture Expérimentale INRA des Monts d'Arrée (PEIMA), Institut National de la Recherche Agronomique (INRA), Syndicat des Sélectionneurs Avicoles et Aquacoles Français, ANR-11-INBS-0003 & Projet BIOGERM Fonds Européens des Affaires Maritimes et de la Pêche (FEAMP), Syndicat des Sélectionneurs Avicoles et Aquacoles Français (SYSAAF), Institut Technique de l'Aviculture et des Elevages de Petits Animaux (ITAVI). FRA., and ANR-11-INBS-0003,CRB-Anim,Réseau de Centres de Ressources Biologiques pour les animaux domestiques(2011)

Subjects
fish, salmonidae, poisson, fécondance des spermatozoides, évolution des pratiques, salmonids, sperme, [SDV]Life Sciences [q-bio], cryofixation, semen, cryoconservation, congelation du sperme, fécondance
Abstract

Session : Reproduction; Cryoconservation du sperme de salmonidés : évolution des pratiques et amélioration des fécondances. 6. Journées de la Recherche Filière Piscicole

Published
2019

8. Régénération de lignées de truite par transplantation de cellules souches germinales

Author

Goupil, Anne-Sophie, Krieg, Francine, Maouche, Ahmed, Depince, Alexandra, Duret, Cécile, Dechamp, Nicolas, Goardon, Lionel, Quillet, Edwige, Le Gac, Florence, Lareyre, Jean-Jacques, Laboratoire de Physiologie et Génomique des Poissons (LPGP), Institut National de la Recherche Agronomique (INRA)-Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique ), Génétique Animale et Biologie Intégrative (GABI), Institut National de la Recherche Agronomique (INRA)-AgroParisTech, Pisciculture Expérimentale INRA des Monts d'Arrée (PEIMA), Institut National de la Recherche Agronomique (INRA), Institut Technique de l'Aviculture et des Elevages de Petits Animaux (ITAVI). FRA., and European Project: 652831,H2020,H2020-INFRAIA-2014-2015,AQUAEXCEL2020(2015)

Subjects
[SDV.GEN]Life Sciences [q-bio]/Genetics, [SDV.GEN.GA]Life Sciences [q-bio]/Genetics/Animal genetics, [SDV]Life Sciences [q-bio], [INFO]Computer Science [cs], ComputingMilieux_MISCELLANEOUS
Abstract

Session : Reproduction; National audience

Published
2019

9. Analyse comparée du niveau de méthylation globale des spermatozoïdes chez des mâles de 9 espèces d’embranchements différents

Author

Depince, Alexandra, Jammes, Hélène, Labbé, Catherine, Laboratoire de Physiologie et Génomique des Poissons (LPGP), Institut National de la Recherche Agronomique (INRA)-Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique ), Biologie du Développement et Reproduction (BDR), École nationale vétérinaire d'Alfort (ENVA)-Institut National de la Recherche Agronomique (INRA), Université Paris Saclay (COmUE), and ANR-11-INBS-0003 (CRB-Anim 2013-2019)

Subjects
[SDV]Life Sciences [q-bio], [INFO]Computer Science [cs], ComputingMilieux_MISCELLANEOUS
Abstract

National audience

Published
2019

10. Characterization of early embryonic cellular defects after somatic cell nuclear transfer in fish

Author

Rouillon, Charlène, Depince, Alexandra, Chenais, Nathalie, Le Bail, Pierre-Yves, Labbé, Catherine, Laboratoire de Physiologie et Génomique des Poissons (LPGP), and Institut National de la Recherche Agronomique (INRA)-Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique )

Subjects
fish, transfer nuclear, early embryonic cellular, [SDV]Life Sciences [q-bio], somatic cell
Abstract

International audience; Cryopreservation of genetic resources is of major interest for the security of biodiversity and the sustainability of the agronomic industry. Cryopreservation of somatic cells is one means of preserving the genome of both parents, but regeneration of functional breeders requires the mastering of somatic cell NT (SCNT). In mammals, SCNT consists of injecting the somatic nuclei into enucleated recipient oocytes to obtain clones that bear the genome of the donor. Cloning in fish presents some advantages over cloning in mammals: oocytes are produced by the hundreds, and development is external. Moreover, embryonic genome activation takes place after 10 mitoses only. These features are favourable to a better understanding of cellular reprogramming of the somatic cell. Nonenucleated oocytes are often used for SCNT, which allows the production of 2n clones bearing only the donor genome when the maternal chromatin is spontaneously removed by an unknown mechanism (25% of cases). This ability can help us understand the fate of the maternal chromatin and the role of its surrounding factors on the cellular behaviour of the somatic cell during the first developmental steps of embryonic development: meiosis resumption (MR) and mitosis. In this study, fin somatic cells and mature oocytes were obtained from a 2-year-old goldfish (Carassius auratus). For SCNT, the whole fin cell was injected into the sperm entry site, and the oocyte was activated by water contact after 30 min to begin the development. Somatic and maternal chromatin behaviour during MR and early mitosis were characterised by immunofluorescence (Hoechst/Vybrant labelling for chromatin identification and α-tubulin for spindle organisation). Variability in chromatin condensation was observed after the somatic cell injection before the activation. Clone analysis (n = 16) revealed that some of them presented a condensed somatic nuclei (71%) and others presented a decondensed nuclei (29%). After activation, most clones underwent normal polar body extrusion of the maternal chromatin (66%, n = 69/95 clones v. 96%, n = 96/100 controls oocytes) without extrusion of a somatic polar body. Afterward, clones that presented a first symmetric division (n = 14/35) were analysed at the 2-cells stage and compared with fertilized embryos (n = 7). The maternal chromatin was observed as fragmented on the cleavage furrow (79%), where it cannot contribute to the development. Spindle defects were observed in 50% of clone cells (v. 0% in controls), such as multicentrosomal spindles, chromosome misalignment, abnormal segregation, and DNA fragmentation. To conclude, those clone (2n or 3n) defects are probably due to the chromatin condensation observed before activation. The fish oocyte volume did not allow the MR of the condensed somatic chromatin, and that may induce an abnormal anaphase and the following clone developmental defects.

Published
2019

11. 7th International Workshop on the Biology of Fish Gametes - 2-6 Sept. 2019, Rennes France. Abstract book

Author

Laurent, Audrey, Depince, Alexandra, Laboratoire de Physiologie et Génomique des Poissons (LPGP), and Institut National de la Recherche Agronomique (INRA)-Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique )

Subjects
[SDV]Life Sciences [q-bio], [INFO]Computer Science [cs], ComputingMilieux_MISCELLANEOUS
Abstract

International audience

Published
2019

12. Somatic cell nuclear transfer in non-enucleated goldfish oocytes: understanding DNA fate during oocyte activation and first cellular division

Author

Rouillon, Charlène, Depince, Alexandra, Chenais, Nathalie, Le Bail, Pierre-Yves, Labbé, Catherine, Laboratoire de Physiologie et Génomique des Poissons (LPGP), Institut National de la Recherche Agronomique (INRA)-Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique ), This work has benefited from the Tefor Fish Phenotyping Platform at the INRA LPGP, Rennes (ANR-II-INBS-0014). This work was funded by the French CRB Anim project, ANR-11-INBS-0003. C.Rouillon was recipient of an INRA PHASE and Région Bretagne PhD fellowship, ANR-11-INBS-0003,CRB-Anim,Réseau de Centres de Ressources Biologiques pour les animaux domestiques(2011), and ANR-11-INBS-0014,TEFOR,Transgenèse pour les Etudes Fonctionnelles sur les Organismes modèles(2011)

Subjects
cell division, clone cellulaire, Nuclear Transfer Techniques, Embryology, nuclear transfer, reprogrammation nucléaire, [SDV]Life Sciences [q-bio], clone cells, lcsh:Medicine, Spindle Apparatus, eye enucleation, division cellulaire, dna, Article, Chromosomes, reproduction, histologie, énucléation, poisson, cyprinidae, Animals, reprogramming of the genome, goldfish, immunofluorescence, lcsh:Science, genome, Metaphase, cell division cycle, fish, génome, lcsh:R, carassius auratus, indirect fluorescent antibody techn, adn, cryoconservation, cycle cellulaire, poisson rouge, transfert nucléaire, cryofixation, Oocytes, lcsh:Q, Cloning
Abstract

Nuclear transfer consists in injecting a somatic nucleus carrying valuable genetic information into a recipient oocyte to sire a diploid offspring which bears the genome of interest. It requires that the oocyte (maternal) DNA is removed. In fish, because enucleation is difficult to achieve, non-enucleated oocytes are often used and disappearance of the maternal DNA was reported in some clones. The present work explores which cellular events explain spontaneous erasure of maternal DNA, as mastering this phenomenon would circumvent the painstaking procedure of fish oocyte enucleation. The fate of the somatic and maternal DNA during oocyte activation and first cell cycle was studied using DNA labeling and immunofluorescence in goldfish clones. Maternal DNA was always found as an intact metaphase within the oocyte, and polar body extrusion was minimally affected after oocyte activation. During the first cell cycle, only 40% of the clones displayed symmetric cleavage, and these symmetric clones contributed to 80% of those surviving at hatching. Maternal DNA was often fragmented and located under the cleavage furrow. The somatic DNA was organized either into a normal mitotic spindle or abnormal multinuclear spindle. Scenarios matching the DNA behavior and the embryo fate are proposed.

Published
2019

13. Somatic cell nuclear transfer in non-enucleated goldfish oocytes: understanding DNA fate during meiosis resumption and first cellular division

Author

Rouillon, Charlène, Depince, Alexandra, Chenais, Nathalie, Le Bail, Pierre-Yves, and Labbé, Catherine

Subjects
énucléation, poisson, cellule somatique, reprogrammation nucléaire, transfert nucléaire, développement embryonnaire, cyprinidae, carassius auratus, clonage cellulaire, survie embryonnaire, ovocyte, immunofluorescence, cryoconservation
Abstract

Nuclear transfer consists in injecting a somatic nucleus carrying valuable genetic information into a recipient oocyte to sire a diploid offspring who bears the genome of interest. It requires that the oocyte (maternal) DNA is removed. In fish, because enucleation is difficult to achieve, non-enucleated oocytes are often used and disappearance of the maternal DNA was reported in some clones. The present work explore which cellular events explain spontaneous erasure of maternal DNA, as mastering this phenomenon would circumvent the painstaking procedure of fish oocyte enucleation. The fate of the somatic and maternal DNA during meiosis resumption and first cell cycle was studied using DNA labeling and immunofluorescence in goldfish clones. Maternal DNA was always found as an intact metaphase within the oocyte, and polar body extrusion was minimally affected after meiosis resumption. During the first cell cycle, only 40 % of the clones displayed symmetric cleavage, and these symmetric clones contributed to 80 % of those surviving at hatching. Maternal DNA was often fragmented and located under the cleavage furrow. The somatic DNA was organized either into a normal mitotic spindle or abnormal multinuclear spindle. Scenarios matching the DNA behavior and the embryo fate are proposed.

Published
2019

14. Meiosis resumption and early mitosis after somatic cell nuclear transfer in non-enucleated goldfish oocytes

Author

Rouillon, Charlène, Depince, Alexandra, Le Bail, Pierre-Yves, Labbé, Catherine, Laboratoire de Physiologie et Génomique des Poissons (LPGP), Institut National de la Recherche Agronomique (INRA)-Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique ), Instituto Nacional de Pesquisas da Amazônia (INPA). Manaus, BRA., Laboratoire de Physiologie et Génomique des Poissons ( LPGP ), and Institut National de la Recherche Agronomique ( INRA ) -Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique )

Subjects
fish, nuclear transfer, [ SDV ] Life Sciences [q-bio], [SDV]Life Sciences [q-bio], carassius auratus, embryo, ovocyte, cryoconservation, méïose, reproduction, transfert nucléaire, cellule somatique, poisson, cyprinidae, embryon, cryofixation, somatic cell, goldfish
Abstract

Introduction: The cryopreservation of genetic resources is of major interest for the security of biodiversity and for the sustainability of agronomic industry. Cryopreservation of somatic cells, which carry the genome of both parents, is one mean to regenerate functional breeders, but this requires the mastering of nuclear transfer. In mammals, somatic cell nuclear transfer consists in injecting the somatic nuclei into an enucleated mature recipient oocyte, in order to obtain clone which bear the genome of interest from the donor animal. However, in fish species, the oocyte structure makes the enucleation step difficult to achieve (presence of the chorion and bulk nutritive reserve), so a non-enucleated oocyte is often used for nuclear transfer. To obtain a real clone and not hybrid embryos, the oocyte chromatin must therefore be spontaneously excluded from the developing embryo. This phenomenon has been described in several studies but the mechanism involved is still unknown. Deciphering this phenomenon requires a high understanding of the first developmental steps that are meiosis resumption and early mitosis. The aim of the study is to understand the interplay between the maternal chromatin and the injected one upon nuclear transfer during these entire critical steps, in order to identify and use means to improve the success rate of nuclear transfer in fish. Methods: Fin somatic cell and mature oocytes were obtained from 2 years old goldfish (Carassius auratus) females. For nuclear transfer, the whole fin cell was injected under the micropyle. The egg activation was induced 30min after cell injection. Somatic and maternal chromatin during meiosis resumption and early mitosis was characterized by immunofluorescence (hoechst and vybrant labelling for chromatin identification and α-tubulin for spindle organization). Results and Discussion: We observed that a high proportion of clone underwent a normal polar body extrusion after activation (60% vs 96 % for the controls). We hypothesize that in most cases, maternal chromatin achieved correctly its meiosis resumption and that the maternal chromatin behavior was not disturbed by nuclear transfer. However, these observations do not augur early mitosis fitness in clone. Indeed, we observed several spindle defects in clone cells: metaphase misalignment, abnormal segregation, and multicentrosomal spindle, associated with DNA fragmentation. Conclusion: Oocyte non-enucleation for SCNT procedure did not drastically alter the meiosis resumption process, but it could be involved in the perturbation of mitosis during clone early development.

Published
2018

15. Utilisation de l’essai des comètes pour la surveillance des milieux aquatiques – problématique de la conservation des échantillons

Author

Taïr-Abbaci, K., Labbé, Catherine, Depince, Alexandra, Garnero, L., Bony, Sylvie, Devaux, Alain, Chaumot, Arnaud, Geffard, O., Laboratoire d'Ecotoxicologie, Institut national de recherche en sciences et technologies pour l'environnement et l'agriculture (IRSTEA), Laboratoire de Physiologie et Génomique des Poissons (LPGP), Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique )-Institut National de la Recherche Agronomique (INRA), Laboratoire d'Ecologie des Hydrosystèmes Naturels et Anthropisés (LEHNA), Université Claude Bernard Lyon 1 (UCBL), and Université de Lyon-Université de Lyon-École Nationale des Travaux Publics de l'État (ENTPE)-Centre National de la Recherche Scientifique (CNRS)

Subjects
substance chimique, espèce sentinelle, bioindicateur, essai comète, gammare, génotoxicité, encagement, spermatozoïde, [SDV]Life Sciences [q-bio], test comete, cryoconservation, milieu aquatique, conservation des echantillons, durée d'exposition, dégradation de l'adn, [INFO]Computer Science [cs], espèce aquacole, écotoxicologie
Abstract

National audience; Les milieux aquatiques constituent le réceptacle d’un grand nombre de contaminants, anthropiques et naturels, pouvant être dangereux pour les organismes qui y vivent.

Published
2018

16. Compréhension des perturbations induites par le transfert nucléaire chez le carassin

Author

Rouillon, Charlène, Depince, Alexandra, Le Bail, Pierre-Yves, Labbé, Catherine, Laboratoire de Physiologie et Génomique des Poissons (LPGP), Institut National de la Recherche Agronomique (INRA)-Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique ), Bourse INRA PHASE-Région Bretagne. Travaux financés pas le Programme Investissements d’Avenir CRB Anim, ANR-11-INBS-0003, and Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique )-Institut National de la Recherche Agronomique (INRA)

Subjects
disturbance, fish, clone, nuclear transfer, méiose, perturbation, [SDV]Life Sciences [q-bio], adn, ovocyte, dna, cryoconservation, poisson rouge, cellule somatique, transfert nucléaire, poisson, cyprinidae, cryofixation, meiosis, somatic cell
Abstract

Compréhension des perturbations induites par le transfert nucléaire chez le carassin. Caractérisation de la reprise de la méiose et des premières mitoses. Journées d'Animation Scientifique du département Phase (JAS Phase 2018)

Published
2018

17. Défauts de reprogrammation épigénétique des embryons issus du transfert nucléaire somatique chez les poissons

Author

Depince, Alexandra, Le Bail, Pierre-Yves, Labbé, Catherine, Laboratoire de Physiologie et Génomique des Poissons (LPGP), Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique )-Institut National de la Recherche Agronomique (INRA), Institut National de la Recherche Agronomique (INRA)-Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique ), Financé par 'Investissements d’Avenir' ANR-11-INBS-0003 (CRB-Anim 2013-2019) et COST AQUAGAMETE FA 1205, and ProdInra, Archive Ouverte

Subjects
fish, nuclear transfer, méthylation de l'adn, [SDV]Life Sciences [q-bio], carassius auratus, adn, embryo, dna, reprogrammation epigénétique, [SDV] Life Sciences [q-bio], poisson rouge, poisson, cellule somatique, transfert nucléaire, cyprinidae, embryon, somatic cell, goldfish, méthylation des gènes
Abstract

Défauts de reprogrammation épigénétique des embryons issus du transfert nucléaire somatique chez les poissons. Journées d'Animation Scientifique du département Phase (JAS Phase 2018)

Published
2018

18. Somatic cell nuclear transfer in rainbow trout (Onrohynchus mykiss): What makes it different from fertilization with a spermatozoa ?

Author

Depince, Alexandra, Le Bail, Pierre-Yves, Labbé, Catherine, Laboratoire de Physiologie et Génomique des Poissons (LPGP), Institut National de la Recherche Agronomique (INRA)-Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique ), and ANR-11-INBS-0003 (CRB-Anim 2013-2019) - COST AQUAGAMETE FA 1205

Subjects
endocrine system, nuclear transfer, animal structures, animal diseases, [SDV]Life Sciences [q-bio], education, bacterialresistance transfer factor, physiologie de la reproduction, reproduction, fertilité, poisson, rainbow, spermatozoa, salmonids, spermatozoïde, fish, salmonidae, trout, oncorhynchus mykiss, urogenital system, cryoconservation, rainbow trout, sex factors, cellule somatique, transfert nucléaire, cryofixation, somatic cell, truite arc en ciel
Abstract

Somatic cell nuclear transfer in rainbow trout (Onrohynchus mykiss): What makes it different from fertilization with a spermatozoa ?. 11. International Symposium on Reproductive Physiology of Fish

Published
2018

19. Epigenetic reprogramming after nuclear transfer in fish

Author

Depince, Alexandra, Le Bail, Pierre-Yves, Labbé, Catherine, Laboratoire de Physiologie et Génomique des Poissons (LPGP), Institut National de la Recherche Agronomique (INRA)-Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique ), Financial support of 'Investissements d’Avenir' ANR-11-INBS-0003 (CRB-Anim 2013–2019) and COST AQUAGAMETE FA 1205, University of South Bohemia in České Budějovice. CZE., and ProdInra, Archive Ouverte

Subjects
fish, nuclear transfer, DNA methylation, méthylation de l'adn, health care facilities, manpower, and services, [SDV]Life Sciences [q-bio], education, epigenetic reprogramming, reprogrammation epigénétique, cryoconservation, somatic cell nuclear transfer, [SDV] Life Sciences [q-bio], poisson rouge, reproduction, oeuf de poisson, poisson, cellule somatique, transfert nucléaire, cyprinidae, cryofixation, somatic cell, goldfish, ComputingMilieux_MISCELLANEOUS, health care economics and organizations, expression des gènes
Abstract

Epigenetic reprogramming after nuclear transfer in fish. 6. International Workshop on the Biology of Fish Gametes

Published
2017

20. Meiosis resumption and early mitosis pattern after somatic cell nuclear transfer in goldfish

Author

Rouillon, Charlène, Depince, Alexandra, Le Bail, Pierre-Yves, Labbé, Catherine, Laboratoire de Physiologie et Génomique des Poissons (LPGP), Institut National de la Recherche Agronomique (INRA)-Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique ), This work is funded by the French CRB Anim project «Investissements d’avenir», ANR- 11-INBS-0003. CR is recipient of an INRA-PHASE and Région Bretagne fellowship., University of South Bohemia in České Budějovice. CZE., ANR-11-INBS-0003,CRB-Anim,Réseau de Centres de Ressources Biologiques pour les animaux domestiques(2011), ProdInra, Archive Ouverte, and Infrastructures - Réseau de Centres de Ressources Biologiques pour les animaux domestiques - - CRB-Anim2011 - ANR-11-INBS-0003 - INBS - VALID

Subjects
nuclear transfer, [SDV]Life Sciences [q-bio], education, embryo, régénération cellulaire, reproduction, poisson, cyprinidae, early mitosis, foetal development, fish, conservation, reprogramming, transition mitose meiose, cryoconservation, mortality, [SDV] Life Sciences [q-bio], poisson rouge, cellule somatique, transfert nucléaire, développement embryonnaire, embryon, cryofixation, somatic cell, sense organs, conservation des ressources génétiques, mortalité
Abstract

Meiosis resumption and early mitosis pattern after somatic cell nuclear transfer in goldfish. 6. International Workshop on the Biology of Fish Gametes

Published
2017

21. Epigenetic stability in goldfish sperm upon hormonal induction and over the breeding season

Author

Depince, Alexandra, Bertin, Chloé, Labbé, Catherine, Laboratoire de Physiologie et Génomique des Poissons (LPGP), Institut National de la Recherche Agronomique (INRA)-Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique ), This work is funded by the French CRB Anim project «Investissements d’avenir», ANR-11-INBS-0003, ProdInra, Archive Ouverte, and University of South Bohemia in České Budějovice. CZE.

Subjects
fish, DNA methylation, sperm quality, urogenital system, méthylation de l'adn, [SDV]Life Sciences [q-bio], education, spermatogenèse, qualité du sperme, spermatogenesis, [SDV] Life Sciences [q-bio], poisson rouge, reproduction, épigénétique, nervous system, poisson, spermatozoa, biotechnologie de la reproduction, cyprinidae, chromatin, spermatozoïde, sense organs, induction hormonale, chromatine, health care economics and organizations
Abstract

Epigenetic stability in goldfish sperm upon hormonal induction and over the breeding season. 6. International Workshop on the Biology of Fish Gametes

Published
2017

22. Single and multiple somatic cells nuclear transfer in critically endangered species, sturgeon

Author

FATIRA, Effrosiny, Labbé, Catherine, Depince, Alexandra, Legorova, Victoriia, Pocherniaieva, Kseniia, Güralp, Hilal, Havelka, Miloš, Pšenička, Martin, Saito, Taiju, Faculty of Fisheries and Protection of Waters, Laboratory of Germ Cells, University of South Bohemia, Laboratoire de Physiologie et Génomique des Poissons (LPGP), Institut National de la Recherche Agronomique (INRA)-Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique ), Faculty of Fisheries Sciences [Hakodate], Hokkaido University [Sapporo, Japan], South Ehime Fisheries Research Center, Ehime University [Matsuyama], The study was supported from Grant Agency of the University of South Bohemia in České Budějovice, GAJU Fatira 068/2017/Z 2017., University of South Bohemia in České Budějovice. CZE., Hokkaido University, and ProdInra, Archive Ouverte

Subjects
fish, acipenseridae, nuclear transfer, [SDV]Life Sciences [q-bio], education, cloning, espèce en danger, mise au point de technique, sturgeon, intraspecies SCNT, chimera, esturgeon commun, [SDV] Life Sciences [q-bio], reproduction, poisson, cellule somatique, transfert nucléaire, species threatened with extinction, clonage, somatic cell, transplantation cellulaire, single and multi fins micro injection, interspecies SCNT
Abstract

Single and multiple somatic cells nuclear transfer in critically endangered species, sturgeon. 6. International Workshop on the Biology of Fish Gametes

Published
2017

23. Optimisation des conditions d’élevage des poissons rouges : obtention de gamètes et réduction du nombre d’animaux expérimentaux

Author

Depince, Alexandra, Laboratoire de Physiologie et Génomique des Poissons (LPGP), Institut National de la Recherche Agronomique (INRA)-Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique ), and ProdInra, Archive Ouverte

Subjects
[SDV] Life Sciences [q-bio], fish, poisson rouge, condition d'élevage, poisson, cyprinidae, [SDV]Life Sciences [q-bio], expérimentation
Abstract

Optimisation des conditions d’élevage des poissons rouges : obtention de gamètes et réduction du nombre d’animaux expérimentaux. 2. Journées des Installations Expérimentales LPGP

Published
2017

24. Epigenetic and cellular reprogramming after nuclear transfer in fish : characterization of clone defects in relation to the donor cell origin

Author

Rouillon, Charlène, Depince, Alexandra, Le Bail, Pierre-Yves, Labbé, Catherine, Laboratoire de Physiologie et Génomique des Poissons (LPGP), Institut National de la Recherche Agronomique (INRA)-Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique ), Centre National de la Recherche Scientifique (CNRS). FRA., and ProdInra, Archive Ouverte

Subjects
fish, nuclear transfer, [SDV]Life Sciences [q-bio], conservation, reprogramming, reprogrammation epigénétique, cryoconservation, [SDV] Life Sciences [q-bio], épigénétique, poisson, cellule somatique, transfert nucléaire, cryofixation, somatic cell, epigenetic
Abstract

Epigenetic and cellular reprogramming after nuclear transfer in fish : characterization of clone defects in relation to the donor cell origin. 2. Journées scientifiques GDR 3606 REPRO : ReproSciences 2017

Published
2017

25. Cryoconservation de la semence de poisson : importance du maintien de l'intégrité épigénétique

Author

Depince, Alexandra, Labbé, Catherine, Laboratoire de Physiologie et Génomique des Poissons (LPGP), Institut National de la Recherche Agronomique (INRA)-Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique ), ANR-11-INBS-003 5 CRB-Anim 2013-2019 - COST AQUAGAMETE FA 1205, Institut Technique de l'Aviculture et des Elevages de Petits Animaux (ITAVI). FRA., and ProdInra, Migration

Subjects
[SDV] Life Sciences [q-bio], [SDV]Life Sciences [q-bio], [INFO]Computer Science [cs], [INFO] Computer Science [cs], ComputingMilieux_MISCELLANEOUS
Abstract

International audience

Published
2016

26. Stemness properties and molecular signature of paired spermatogonia in adult zebrafish

Author

Curran, Edouard, Gautier, Aude, Saito, Taiju, Patinote, Amélie, Depince, Alexandra, Labbé, Catherine, Chalmel, Frédéric, Le Gac, Florence, Lareyre, Jean-Jacques, Laboratoire de Physiologie et Génomique des Poissons (LPGP), Institut National de la Recherche Agronomique (INRA)-Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique ), University of South Bohemia, Institut National de la Santé et de la Recherche Médicale (INSERM), ProdInra, Migration, Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique )-Institut National de la Recherche Agronomique (INRA), Institut de recherche en santé, environnement et travail (Irset), Université d'Angers (UA)-Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES)-École des Hautes Études en Santé Publique [EHESP] (EHESP)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique ), and Université d'Angers (UA)-Université de Rennes (UR)-École des Hautes Études en Santé Publique [EHESP] (EHESP)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique )

Subjects
[SDV] Life Sciences [q-bio], [SDV]Life Sciences [q-bio], [INFO]Computer Science [cs], [INFO] Computer Science [cs], ComputingMilieux_MISCELLANEOUS
Abstract

International audience

Published
2016

27. Isolement et caractérisation de cellules germinales souches adultes à partir de lignées de poissons zèbre transgéniques

Author

Curran, Edouard, Gautier, Aude, Saito, Taiju, Patinote, Amélie, Depince, Alexandra, Labbé, Catherine, Pain, Bertrand, Chalmel, Frédéric, Le Gac, Florence, Lareyre, Jean-Jacques, ProdInra, Migration, Laboratoire de Physiologie et Génomique des Poissons (LPGP), Institut National de la Recherche Agronomique (INRA)-Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique ), University of Bohemia, Partenaires INRAE, Institut cellule souche et cerveau (U846 Inserm - UCBL1), Institut National de la Recherche Agronomique (INRA)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut National de la Santé et de la Recherche Médicale (INSERM), and Institut National de la Santé et de la Recherche Médicale (INSERM)

Subjects
[SDV] Life Sciences [q-bio], [SDV]Life Sciences [q-bio], [INFO]Computer Science [cs], [INFO] Computer Science [cs], cellule germinale souche, régénération, transcriptome, ComputingMilieux_MISCELLANEOUS, transplantation
Abstract

International audience

Published
2016

28. Les cellules somatiques : un intérêt pour la conservation des ressources génétiques chez les poissons

Author

Chenais, Nathalie, Depince, Alexandra, Le Bail, Pierre-Yves, Labbé, Catherine, ProdInra, Migration, Laboratoire de Physiologie et Génomique des Poissons (LPGP), Institut National de la Recherche Agronomique (INRA)-Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique ), Société Fançaise d'Ichtyologie. FRA., and Institut Technique de l'Aviculture et des Elevages de Petits Animaux (ITAVI). FRA.

Subjects
cellules nageoire, [SDV]Life Sciences [q-bio], biotechnologie animale, [INFO] Computer Science [cs], cryoconservation, ressource génétique, [SDV] Life Sciences [q-bio], nageoire, information génétique, poisson, cellule somatique, transfert nucléaire, biotechnologie de la reproduction, culture primaire, [INFO]Computer Science [cs], reprogrammation, ComputingMilieux_MISCELLANEOUS, expression des gènes
Abstract

International audience

Published
2016

29. Reprogrammation épigénétique après transfert nucléaire chez les poissons

Author

Depince, Alexandra, Le Bail, Pierre-Yves, Labbé, Catherine, ProdInra, Migration, Laboratoire de Physiologie et Génomique des Poissons (LPGP), Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique )-Institut National de la Recherche Agronomique (INRA), and Institut National de Recherche Agronomique (INRA). UAR Département Physiologie Animale et Systèmes d'Elevage (0558).

Subjects
[SDV] Life Sciences [q-bio], [SDV]Life Sciences [q-bio], [INFO]Computer Science [cs], [INFO] Computer Science [cs], ComputingMilieux_MISCELLANEOUS
Abstract

National audience

Published
2016

30. Reprogramming defects after nuclear transfer in fish

Author

Depince, Alexandra, Le Bail, Pierre-Yves, Chenais, Nathalie, Jammes, Hélène, Labbé, Catherine, Laboratoire de Physiologie et Génomique des Poissons (LPGP), Institut National de la Recherche Agronomique (INRA)-Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique ), Biologie du Développement et Reproduction (BDR), École nationale vétérinaire d'Alfort (ENVA)-Institut National de la Recherche Agronomique (INRA), ANR-11-INBS-0003 (CRB-Anim 2013-2019) - COST Aquagamete FA1205, and European Cooperation in Science and Technology (COST). BEL.

Subjects
[SDV]Life Sciences [q-bio], [INFO]Computer Science [cs]
Abstract

International audience; Somatic cells bear a major potential as cell resource for biodiversity conservation: they carry the genome of both parents, and they can be collected whatever the age or sex of the donor. In fish, cryobanking of somatic cells is highly valuable because eggs and embryos cannot be cryopreserved. Then, the sole use of cryopreserved sperm for animal restoration requires long back-crossing with fresh eggs from plain strains. With somatic cells, the regeneration of the donor genotype will however rely on the nuclear transfer technology in which the donor cell nucleus is injected into a recipient oocyte. Although this restoration technology was developed in the 60’ for fish, the method is still far from being reliable enough for genome preservation and population restoration. Using the goldfish as a model, we showed that the most critical stage after nuclear transfer was the mid-blastula transition stage, when the 1000 cells embryo triggers its own genome activation. This strongly suggests that although the injected nucleus faced 10 rounds of mitosis, chromatin reprogramming was not reliable enough to allow the proper expression of the early development genes. We demonstrated that several marker genes whose promoter regions are hypomethylated in fertilized early embryos displayed various patterns of hypermethylation which were closer to the donor cell pattern. To some extent, the morphology of the embryo could be correlated to the level of the DNA methylation reprogramming. This demonstration reinforces the need to reprogram the donor cell prior to or during nuclear transfer, and the best strategies when facing fish cells and embryos will be discussed.

Published
2015

31. Development of cryopreservation of zebrafish (Danio rerio) sperm

Author

Kollar, Timea, Horvath, Akos, Labesse, Charlène, Milon, Pierre, Csenki, Zsolt, Kovács, Róbert, Bernáth, Gergely, Béres, Tibor, Depince, Alexandra, Urbányi, Béla, Labbé, Catherine, Department of Aquaculture, Szent István University, Laboratoire de Physiologie et Génomique des Poissons (LPGP), Institut National de la Recherche Agronomique (INRA)-Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique ), COST Action FA 1205 AQUAGAMETE, Università Politecnica delle Marche. ITA., and ProdInra, Migration

Subjects
[SDV] Life Sciences [q-bio], [SDV]Life Sciences [q-bio], [INFO]Computer Science [cs], [INFO] Computer Science [cs], ComputingMilieux_MISCELLANEOUS
Abstract

International audience

Published
2015

32. Cryoprotectants alter DNA methylation pattern of spermatozoa and decrease reproductive success in Colossoma macropomum

Author

de Mello, Fernanda, Garcia, Juliana Saraiva, de Godoy, Leandro C., Depince, Alexandra, Labbé, Catherine, Streit, Danilo P. Jr., Laboratory of Aquaculture, Universidade Federal do Rio Grande do Sul [Porto Alegre] (UFRGS), Federal University of Rio Grande do Sul, Laboratory of Biotechnology Applied to Fish Reproduction, Nilton Lins University, Laboratoire de Physiologie et Génomique des Poissons (LPGP), Institut National de la Recherche Agronomique (INRA)-Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique ), COST Action FA 1205 AQUAGAMETE, and Università Politecnica delle Marche. ITA.

Subjects
[SDV]Life Sciences [q-bio], [INFO]Computer Science [cs], ComputingMilieux_MISCELLANEOUS
Abstract

International audience

Published
2015

33. Molecular signature and stemness properties of paired spermatogonia in zebrafish

Author

Curran, Edouard, Gautier, Aude, Saito, Taiju, Patinote, Amélie, Depince, Alexandra, Labbé, Catherine, Le Gac, Florence, Lareyre, Jean-Jacques, Laboratoire de Physiologie et Génomique des Poissons (LPGP), Institut National de la Recherche Agronomique (INRA)-Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique ), University of South Bohemia, COST Action FA 1205 AQUAGAMETE, Università Politecnica delle Marche. ITA., and ProdInra, Migration

Subjects
[SDV] Life Sciences [q-bio], [SDV]Life Sciences [q-bio], [INFO]Computer Science [cs], [INFO] Computer Science [cs], ComputingMilieux_MISCELLANEOUS
Abstract

International audience

Published
2015

34. Impact de la cryoconservation du sperme sur la méthylation de l'ADN : l'effet espèce chez les poissons

Author

Labbé, Catherine, Depince, Alexandra, Millon, Pierre, Gabory, Anne, Jammes, Hélène, Laboratoire de Physiologie et Génomique des Poissons (LPGP), Institut National de la Recherche Agronomique (INRA)-Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique ), Biologie du Développement et Reproduction (BDR), École nationale vétérinaire d'Alfort (ENVA)-Institut National de la Recherche Agronomique (INRA), and Institut National de Recherche Agronomique (INRA). UAR Département Physiologie Animale et Systèmes d'Elevage (0558).

Subjects
[SDV]Life Sciences [q-bio], [INFO]Computer Science [cs], ComputingMilieux_MISCELLANEOUS
Abstract

National audience

Published
2015

35. Clonage et transplantation de cellules germinales, pour la conservation des ressources génétiques

Author

Depince, Alexandra, ProdInra, Migration, Laboratoire de Physiologie et Génomique des Poissons (LPGP), and Institut National de la Recherche Agronomique (INRA)-Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique )

Subjects
[SDV] Life Sciences [q-bio], [SDV]Life Sciences [q-bio], [INFO]Computer Science [cs], [INFO] Computer Science [cs], ComputingMilieux_MISCELLANEOUS
Abstract

National audience

Published
2015

36. Méthylation globale de la semence de poisson rouge après cryoconservation. Effet des cryoprotecteurs

Author

Depince, Alexandra, Labbé, Catherine, Laboratoire de Physiologie et Génomique des Poissons (LPGP), Institut National de la Recherche Agronomique (INRA)-Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique ), and ProdInra, Migration

Subjects
[SDV.OT]Life Sciences [q-bio]/Other [q-bio.OT], [SDV.OT] Life Sciences [q-bio]/Other [q-bio.OT], ComputingMilieux_MISCELLANEOUS
Abstract

National audience

Published
2014

37. Cryopreserving European eel (A. anguilla) sperm: comparison of two methods for standardization

Author

Vilchez, Maria C., Morini, Marina, Penaranda, David S., Pérez, Luz, Depince, Alexandra, Kasa, Esztzer, Labbé, Catherine, Horvath, Akos, Asturiano, Juan F., Universitat Politècnica de València (UPV), Instituto de Ciencia y Tecnologia Animal, Partenaires INRAE, Laboratoire de Physiologie et Génomique des Poissons (LPGP), Institut National de la Recherche Agronomique (INRA)-Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique ), Szent István University, CRB-Anim project 'Investissements d'Avenir 'ANR-11-INBS-0003 & COST Action FA1205: AQUAGAMETE, European Project: 245257,EC:FP7:KBBE,FP7-KBBE-2009-3,PRO-EEL(2010), and Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique )-Institut National de la Recherche Agronomique (INRA)

Subjects
poisson, cryoconservation du sperme, méthylation de l'adn, anguille, [SDV]Life Sciences [q-bio], anguilla anguilla, méthode de conservation, [INFO]Computer Science [cs], cryoconservation, ComputingMilieux_MISCELLANEOUS
Abstract

International audience

Published
2014

38. Dna methylation of fish germ cells and the risk of alteration after cryopreservation

Author

Labbé, Catherine, Depince, Alexandra, Milon, Pierre, Morini, M., Riesco, M., Robles, V., Asturiano, J., Horvath, A., Herraez, P., Laboratoire de Physiologie et Génomique des Poissons (LPGP), Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique )-Institut National de la Recherche Agronomique (INRA), Physiologie de la reproduction et des comportements [Nouzilly] (PRC), Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours (UT)-Centre National de la Recherche Scientifique (CNRS), Institute of Animal Science and Technology, Universitat Politècnica de València (UPV), Universidad de León [León], Department of Aquaculture, Szent István University, Department of Molecular Biology, University of Leon, CRB-Anim project 'Investissements d'Avenir' ANR-11-INBS-0003, Center of Marine Sciences (CCMAR). PRT., Institut National de la Recherche Agronomique (INRA)-Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique ), Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours-Centre National de la Recherche Scientifique (CNRS), and ProdInra, Migration

Subjects
[SDV.OT]Life Sciences [q-bio]/Other [q-bio.OT], [SDV.OT] Life Sciences [q-bio]/Other [q-bio.OT], ComputingMilieux_MISCELLANEOUS
Abstract

International audience

Published
2014

39. DNA methylation pattern in fish spermatozoa after cryopreservation: a species specific alteration in cyprinids

Author

Labbé, Catherine, Depince, Alexandra, Milon, Pierre, Jammes, Hélène, Gabory, Anne, Laboratoire de Physiologie et Génomique des Poissons (LPGP), Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique )-Institut National de la Recherche Agronomique (INRA), Physiologie de la reproduction et des comportements [Nouzilly] (PRC), Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours (UT)-Centre National de la Recherche Scientifique (CNRS), Biologie du développement et reproduction (BDR), Centre National de la Recherche Scientifique (CNRS)-École nationale vétérinaire d'Alfort (ENVA)-Institut National de la Recherche Agronomique (INRA), ANR-11-INBS-0003, European Cooperation in Science and Technology (COST). BEL., Institut National de la Recherche Agronomique (INRA)-Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique ), Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours-Centre National de la Recherche Scientifique (CNRS), École nationale vétérinaire d'Alfort (ENVA)-Institut National de la Recherche Agronomique (INRA)-Centre National de la Recherche Scientifique (CNRS), and ProdInra, Migration

Subjects
[SDV.OT]Life Sciences [q-bio]/Other [q-bio.OT], [SDV.OT] Life Sciences [q-bio]/Other [q-bio.OT], ComputingMilieux_MISCELLANEOUS
Abstract

International audience

Published
2014

40. DNA methylation pattern after cryopreservation of goldfish cells: influence of the type of cryoprotectant

Author

Depince, Alexandra, Jammes, Hélène, Gabory, Anne, Labbé, Catherine, Laboratoire de Physiologie et Génomique des Poissons (LPGP), Institut National de la Recherche Agronomique (INRA)-Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique ), Biologie du développement et reproduction (BDR), École nationale vétérinaire d'Alfort (ENVA)-Institut National de la Recherche Agronomique (INRA)-Centre National de la Recherche Scientifique (CNRS), ANR-11-INBS-0003, and European Cooperation in Science and Technology (COST). BEL.

Subjects
[SDV.OT]Life Sciences [q-bio]/Other [q-bio.OT], ComputingMilieux_MISCELLANEOUS
Abstract

International audience

Published
2014

41. Insight into the DNA methylation of fish germ cells, into the risk of alteration after cryopreservation, and possible consequences on embryo development

Author

Labbé, Catherine, Robles, Vanesa, Depince, Alexandra, Riesco, Martin, F., Milon, Pierre, Conradt, Marie, Le Bail, Pierre-Yves, Herraez, Paz, Laboratoire de Physiologie et Génomique des Poissons (LPGP), Institut National de la Recherche Agronomique (INRA)-Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique ), Universidad de León [León], and ProdInra, Migration

Subjects
[SDV.OT]Life Sciences [q-bio]/Other [q-bio.OT], [SDV.OT] Life Sciences [q-bio]/Other [q-bio.OT], ComputingMilieux_MISCELLANEOUS
Abstract

International audience

Published
2013

42. Changes in sperm DNA methylation after cryopreservation in fish

Author

Labbé, Catherine, Conradt, Marie, Depince, Alexandra, Gabory, Anne, Jammes, Helene, Le Bail, Pierre-Yves, Station commune de Recherches en Ichtyophysiologie, Biodiversité et Environnement (SCRIBE), Institut National de la Recherche Agronomique (INRA)-IFR140, Biologie du Développement et Reproduction (BDR), Institut National de la Recherche Agronomique (INRA), Investissements d'Avenir, ANR-11-INBS-0003, Laboratoire de Physiologie et Génomique des Poissons (LPGP), Institut National de la Recherche Agronomique (INRA)-Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique ), Biologie du développement et reproduction (BDR), and École nationale vétérinaire d'Alfort (ENVA)-Institut National de la Recherche Agronomique (INRA)-Centre National de la Recherche Scientifique (CNRS)

Subjects
[SDV.BA]Life Sciences [q-bio]/Animal biology, [SDV.BDLR]Life Sciences [q-bio]/Reproductive Biology
Abstract

absent

Published
2013

43. Rôle de la TSA dans le maintien de la dé‐différentiation des cellules embryonnaires isolées chez le poisson rouge

Author

Le Bail, Pierre-Yves, Chenais, Nathalie, Depince, Alexandra, Labbé, Catherine, ProdInra, Migration, Laboratoire de Physiologie et Génomique des Poissons (LPGP), Institut National de la Recherche Agronomique (INRA)-Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique ), and Institut National de Recherche Agronomique (INRA). UAR Département Physiologie Animale et Systèmes d'Elevage (0558).

Subjects
[SDV] Life Sciences [q-bio], [SDV]Life Sciences [q-bio], [INFO]Computer Science [cs], [INFO] Computer Science [cs], ComputingMilieux_MISCELLANEOUS
Abstract

National audience

Published
2013

44. Le transfert nucléaire chez les poissons : promesses et difficultés pour la régénération d'un patrimoine génétique diploïde cryoconservé

Author

Labbé, Catherine, Depince, Alexandra, Chenais, Nathalie, Marandel, Lucie, Mella Alvarado, Vanessa, Gautier, Laurent, Le Bail, Pierre-Yves, Laboratoire de Physiologie et Génomique des Poissons (LPGP), Institut National de la Recherche Agronomique (INRA)-Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique ), and ProdInra, Migration

Subjects
[SDV.BIO]Life Sciences [q-bio]/Biotechnology, ComputingMilieux_MISCELLANEOUS, [SDV.BIO] Life Sciences [q-bio]/Biotechnology
Abstract

National audience

Published
2012

45. Goldfish embryo development after somatic cell nuclear transfer in non-enucleated oocytes: first mitosis profile, ploidy status, and genetic conformity

Author

Labbé, Catherine, Depince, Alexandra, Chenais, Nathalie, Marandel, Lucie, Le Bail, Pierre-Yves, Station commune de Recherches en Ichtyophysiologie, Biodiversité et Environnement (SCRIBE), Institut National de la Recherche Agronomique (INRA), Institut Fédératif de Recherche - Génétique Fonctionnelle Agronomie et Santé (IFR 140 GFAS), Plateforme Génomique Santé Biogenouest®, and ProdInra, Migration

Subjects
[SDV] Life Sciences [q-bio], [SDV]Life Sciences [q-bio], [INFO]Computer Science [cs], [INFO] Computer Science [cs], ComputingMilieux_MISCELLANEOUS
Abstract

International audience

Published
2011

46. Nuclear transfer with cryopreserved fin cells, a unique but difficult means to reconstruct fish with diploid cells

Author

Labbé, Catherine, Le Bail, Pierre-Yves, Depince, Alexandra, Mella, Vanessa, Chenais, Nathalie, ProdInra, Migration, Station commune de Recherches en Ichtyophysiologie, Biodiversité et Environnement (SCRIBE), Institut National de la Recherche Agronomique (INRA), Institut Fédératif de Recherche - Génétique Fonctionnelle Agronomie et Santé (IFR 140 GFAS), and Plateforme Génomique Santé Biogenouest®

Subjects
[SDV] Life Sciences [q-bio], epigenetics, aquatic species, [SDV]Life Sciences [q-bio], cryobanking, microinjection, reprogramming, [INFO]Computer Science [cs], embryo development, GENETIQUE, [INFO] Computer Science [cs], ComputingMilieux_MISCELLANEOUS, biotechnology
Abstract

International audience

Published
2011

47. DNA methylation pattern in sperm and somatic cells after cryopreservation: Influence of the cryoprotectant

Author

Gautier, Laurent, Depince, Alexandra, Chenais, Nathalie, Marandel, Lucie, Le Bail, Pierre-Yves, Labbé, Catherine, ProdInra, Migration, Station commune de Recherches en Ichtyophysiologie, Biodiversité et Environnement (SCRIBE), Institut National de la Recherche Agronomique (INRA), Institut Fédératif de Recherche - Génétique Fonctionnelle Agronomie et Santé (IFR 140 GFAS), and Plateforme Génomique Santé Biogenouest®

Subjects
[SDV] Life Sciences [q-bio], [SDV]Life Sciences [q-bio], [INFO]Computer Science [cs], [INFO] Computer Science [cs], ComputingMilieux_MISCELLANEOUS
Abstract

International audience

Published
2011

48. Obtention de cellules de nageoire en culture à partir de fragments de nageoire cryoconservés chez le carassin : une stratégie fiable de conservation du génome

Author

Moritz, Charlotte, Depince, Alexandra, Labbé, Catherine, Station commune de Recherches en Ichtyophysiologie, Biodiversité et Environnement (SCRIBE), Institut National de la Recherche Agronomique (INRA), and ProdInra, Migration

Subjects
[SDV] Life Sciences [q-bio], [SDV]Life Sciences [q-bio], [INFO]Computer Science [cs], [INFO] Computer Science [cs], CARASSIUS AURATUS, ComputingMilieux_MISCELLANEOUS
Abstract

National audience

Published
2007

49. Optimisation des conditions de manipulation des ovules en vue du transfert nucléaire chez le carassin

Author

Chenais, Nathalie, Mahé, Sophie, Vignon, Xavier, Depince, Alexandra, Bobe, Julien, Le Bail, Pierre-Yves, Labbé, Catherine, Station commune de Recherches en Ichtyophysiologie, Biodiversité et Environnement (SCRIBE), Institut National de la Recherche Agronomique (INRA), Biologie du développement et reproduction (BDR), and Centre National de la Recherche Scientifique (CNRS)-École nationale vétérinaire d'Alfort (ENVA)-Institut National de la Recherche Agronomique (INRA)

Subjects
[SDV]Life Sciences [q-bio], [INFO]Computer Science [cs], CARASSIUS AURATUS, ComputingMilieux_MISCELLANEOUS
Abstract

National audience

Published
2007

50. A preliminary approach for nuclear transfer in goldfish: reduction in the variability of egg quality, optimization of egg manipulation

Author

Chenais, Nathalie, Mahé, Sophie, Vignon, Xavier, Depince, Alexandra, Bobe, Julien, Le Bail, Pierre-Yves, Labbé, Catherine, Station commune de Recherches en Ichtyophysiologie, Biodiversité et Environnement (SCRIBE), Institut National de la Recherche Agronomique (INRA), Biologie du développement et reproduction (BDR), and Centre National de la Recherche Scientifique (CNRS)-École nationale vétérinaire d'Alfort (ENVA)-Institut National de la Recherche Agronomique (INRA)

Subjects
late blastula, fertilization, embryonic structures, [SDV.BDLR]Life Sciences [q-bio]/Reproductive Biology, egg storage, development, [SDV.BDD]Life Sciences [q-bio]/Development Biology
Abstract

National audience; The development of nuclear transfer in Goldfish (Carassius auratus) requires egg handling to be optimized 1) to maintain the ability of the egg to withstand embryo development 2) to allow micromanipulation in order to enucleate the egg and inject the donor nucleus 3) to favour egg cytoplasm ability for nucleus reprogramming. In this work, several egg-handling conditions were tested and optimized in the context of nuclear transfer experiments. To obtain the best egg quality, eggs should be left in the female and stripped out just before use, as their ability to withstand embryo development decreased after in vitro storage at 4 or 20 degrees C. Egg enucleation required that the chorion be removed by enzymatic digestion, and a fast method is described which allowed chorion removal within 1 min post activation. After activation, polar body extrusion should be used as an indicator of the pronucleus position underneath, due to failure to label egg DNA. The enucleation success remained low. In experiments where the enucleation proccess was not carried out, chorion removal was therefore not necessary. In this case, egg incubation conditions preventing egg activation were developed in order to improve nucleus reprogramming after injection of eggs with high MPF levels. Trout coelomic fluid proved to be the best inactivation medium when compared to the proteasome inhibitor MG132 and to the soybean trypsin inhibitor. In our experimental conditions, clones were obtained with embryonic and somatic nuclei, but none developed further than the onset of zygotic transcription (1000 cells stage).

Published
2007

Catalog

Books, media, physical & digital resources
See catalog results