Search

Your search keyword '"Derambure, Céline"' showing total 133 results
Start Over Author "Derambure, Céline"
133 results on '"Derambure, Céline"'

5. Prenatal Alcohol Exposure Impairs the Placenta–Cortex Transcriptomic Signature, Leading to Dysregulation of Angiogenic Pathways

Author

Sautreuil, Camille, primary, Lecointre, Maryline, additional, Derambure, Céline, additional, Brasse-Lagnel, Carole, additional, Leroux, Philippe, additional, Laquerrière, Annie, additional, Nicolas, Gaël, additional, Gil, Sophie, additional, Savage, Daniel D., additional, Marret, Stéphane, additional, Marguet, Florent, additional, Falluel-Morel, Anthony, additional, and Gonzalez, Bruno J., additional

Published
2023
Full Text
View/download PDF

7. Assessment of branch point prediction tools to predict physiological branch points and their alteration by variants

Author

Leman, Raphaël, Tubeuf, Hélène, Raad, Sabine, Tournier, Isabelle, Derambure, Céline, Lanos, Raphaël, Gaildrat, Pascaline, Castelain, Gaia, Hauchard, Julie, Killian, Audrey, Baert-Desurmont, Stéphanie, Legros, Angelina, Goardon, Nicolas, Quesnelle, Céline, Ricou, Agathe, Castera, Laurent, Vaur, Dominique, Le Gac, Gérald, Ka, Chandran, Fichou, Yann, Bonnet-Dorion, Françoise, Sevenet, Nicolas, Guillaud-Bataille, Marine, Boutry-Kryza, Nadia, Schultz, Inès, Caux-Moncoutier, Virginie, Rossing, Maria, Walker, Logan C., Spurdle, Amanda B., Houdayer, Claude, Martins, Alexandra, and Krieger, Sophie

Published
2020
Full Text
View/download PDF

8. Deep intronic NIPBL de novo mutations and differential diagnoses revealed by whole genome and RNA sequencing in Cornelia de Lange syndrome patients

Author

Coursimault, Juliette, primary, Cassinari, Kévin, additional, Lecoquierre, François, additional, Quenez, Olivier, additional, Coutant, Sophie, additional, Derambure, Céline, additional, Vezain, Myriam, additional, Drouot, Nathalie, additional, Vera, Gabriella, additional, Schaefer, Elise, additional, Philippe, Anaïs, additional, Doray, Bérénice, additional, Lambert, Laëtitia, additional, Ghoumid, Jamal, additional, Smol, Thomas, additional, Rama, Mélanie, additional, Legendre, Marine, additional, Lacombe, Didier, additional, Fergelot, Patricia, additional, Olaso, Robert, additional, Boland, Anne, additional, Deleuze, Jean‐François, additional, Goldenberg, Alice, additional, Saugier‐Veber, Pascale, additional, and Nicolas, Gaël, additional

Published
2022
Full Text
View/download PDF

9. Blood functional assay for rapid clinical interpretation of germline TP53 variants

Author

Raad, Sabine, Rolain, Marion, Coutant, Sophie, Derambure, Céline, Lanos, Raphael, Charbonnier, Françoise, Bou, Jacqueline, Bouvignies, Emilie, Lienard, Gwendoline, Vasseur, Stéphanie, Farrell, Michael, Ingster, Olivier, Baert Desurmont, Stéphanie, Kasper, Edwige, Bougeard, Gaëlle, Frébourg, Thierry, Tournier, Isabelle, Génomique et Médecine Personnalisée du Cancer et des Maladies Neuropsychiatriques (GPMCND), Université de Rouen Normandie (UNIROUEN), and Normandie Université (NU)-Normandie Université (NU)-Institut National de la Santé et de la Recherche Médicale (INSERM)

Subjects
Adult, Male, Adolescent, Genotype, DNA Mutational Analysis, clinical laboratory techniques, Reproducibility of Results, Middle Aged, Polymorphism, Single Nucleotide, genetic testing, methods, Young Adult, [SDV.GEN.GH]Life Sciences [q-bio]/Genetics/Human genetics, Child, Preschool, Neoplasms, Leukocytes, Mononuclear, Cancer Genetics, Humans, Female, Genetic Predisposition to Disease, Tumor Suppressor Protein p53, Child, Germ-Line Mutation, Aged
Abstract

Background The interpretation of germline TP53 variants is critical to ensure appropriate medical management of patients with cancer and follow-up of variant carriers. This interpretation remains complex and is becoming a growing challenge considering the exponential increase in TP53 tests. We developed a functional assay directly performed on patients’ blood. Methods Peripheral blood mononuclear cells were cultured, activated, exposed to doxorubicin and the p53-mediated transcriptional response was quantified using reverse transcription–multiplex ligation probe amplification and RT-QMPSF assays, including 10 p53 targets selected from transcriptome analysis, and two amplicons to measure p53 mRNA levels. We applied this blood functional assay to 77 patients addressed for TP53 analysis. Results In 51 wild-type TP53 individuals, the mean p53 functionality score was 12.7 (range 7.5–22.8). Among eight individuals harbouring likely pathogenic or pathogenic variants, the scores were reduced (mean 4.8, range 3.1–7.1), and p53 mRNA levels were reduced in patients harbouring truncating variants. We tested 14 rare unclassified variants (p.(Pro72His), p.(Gly105Asp), p.(Arg110His), p.(Phe134Leu), p.(Arg158Cys), p.(Pro191Arg), p.(Pro278Arg), p.(Arg283Cys), p.(Leu348Ser), p.(Asp352Tyr), p.(Gly108_Phe109delinsVal), p.(Asn131del), p.(Leu265del), c.-117G>T) and 12 yielded functionally abnormal scores. Remarkably, the assay revealed that the c.*1175A>C polymorphic variant within TP53 poly-adenylation site can impact p53 function with the same magnitude as a null variant, when present on both alleles, and may act as a modifying factor in pathogenic variant carriers. Conclusion This blood p53 assay should therefore be a useful tool for the rapid clinical classification of germline TP53 variants and detection of non-coding functional variants.

Published
2020

11. TRIM33 gene somatic mutations identified by next generation sequencing in neoplasms of patients with anti-TIF1γ positive cancer-associated dermatomyositis

Author

Cordel, Nadège, Derambure, Céline, Coutant, Sophie, Mariette, Xavier, Jullien, Denis, Debarbieux, Sébastien, Chosidow, Olivier, Meyer, Alain, Bessis, Didier, Joly, Pascal, Mathian, Alexis, Levesque, Hervé, Sabourin, Jean-Christophe, Tournier, Isabelle, Boyer, Olivier, Amoura, Zahir, Aouizerate, Jessie, Benveniste, Olivier, Brocheriou, Isabelle, Dechelotte, Benoît, Graveleau, Julie, Guerzider, Pascale, Isaac, Sylvie, Kanitakis, Jean, Lannes, Béatrice, Lazure, Thierry, Rigau, Valérie, Sibilia, Jean, CHU Pointe-à-Pitre/Abymes [Guadeloupe], Physiopathologie, Autoimmunité, maladies Neuromusculaires et THErapies Régénératrices (PANTHER), Université de Rouen Normandie (UNIROUEN), Normandie Université (NU)-Normandie Université (NU)-Institut National de la Santé et de la Recherche Médicale (INSERM), Génomique et Médecine Personnalisée du Cancer et des Maladies Neuropsychiatriques (GPMCND), AP-HP Hôpital Bicêtre (Le Kremlin-Bicêtre), Hôpital Edouard Herriot [CHU - HCL], Hospices Civils de Lyon (HCL), Hôpital Henri Mondor, Dynamic Microbiology - EA 7380 (DYNAMIC), École nationale vétérinaire - Alfort (ENVA)-Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail (ANSES)-Université Paris-Est (UPE)-Université Paris-Est Créteil Val-de-Marne - Paris 12 (UPEC UP12), CHU Strasbourg, Centre Hospitalier Régional Universitaire [Montpellier] (CHRU Montpellier), Pathogenesis and Control of Chronic and Emerging Infections (PCCEI), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Montpellier (UM)-Université des Antilles (UA)-Etablissement français du don du sang [Montpellier], CHU Rouen, Normandie Université (NU), Service de Département de médecine interne et immunologie clinique [CHU Pitié-Salpêtrière] (DMIIC), CHU Pitié-Salpêtrière [AP-HP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU), Institut E3M [CHU Pitié-Salpêtrière], Service de Médecine Interne [CHU Rouen], Normandie Université (NU)-Normandie Université (NU)-Université de Rouen Normandie (UNIROUEN), Institut des Neurosciences de Montpellier (INM), and Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Montpellier (UM)

Subjects
0301 basic medicine, Myositis and muscle disease, Male, Somatic cell, DNA Mutational Analysis, Disease, Gene mutation, MESH: Dermatomyositis, 0302 clinical medicine, Neoplasms, Medicine, Pharmacology (medical), MESH: Neoplasms, MESH: DNA Mutational Analysis, MESH: High-Throughput Nucleotide Sequencing, Skin, MESH: Aged, MESH: DNA, High-Throughput Nucleotide Sequencing, Zinc Fingers, MESH: Transcription Factors, [SDV.MHEP.RSOA]Life Sciences [q-bio]/Human health and pathology/Rhumatology and musculoskeletal system, Muscle, Female, MESH: Mutation, DNA sequencing, Dermatomyositis, 03 medical and health sciences, Rheumatology, Genetics, MESH: Zinc Fingers, Humans, Gene, Aged, 030203 arthritis & rheumatology, MESH: Humans, Neoplasia, business.industry, Cancer, DNA, medicine.disease, TRIM33, MESH: Male, 030104 developmental biology, Mutation, Cancer research, business, MESH: Female, Transcription Factors
Abstract

Objective To deep sequence the TRIM33 gene in tumours from patients with cancer-associated anti-TIF1γ autoantibody-positive dermatomyositis (DM) as TRIM33 somatic mutations in tumours may trigger this auto-immune disease. Methods Next generation sequencing of tumour DNA samples from patients with cancer-associated anti-TIF1γ autoantibody-positive DM. Fourteen tumours from 13 anti-TIF1γ autoantibody-positive DM individuals were sequenced along with two control tumours from non-DM individuals. Results Fourteen probable somatic variants from four tumours were identified in the TRIM33 gene. Conclusion These results are in accordance with the previous report of Pinal-Fernandez et al. and support the hypothesis of a role of TRIM33 gene mutations in the pathophysiology of anti-TIF1γ autoantibody-positive DM.

Published
2021

13. Modifications of the Transcriptomic Profile of Autoreactive BCells From Pemphigus Patients After Treatment With Rituximab or a StandardCorticosteroid Regimen

Author

Vivien, Hébert, Marie, Petit, Maho-Vaillant, Maud, Marie-Laure, Golinski, Derambure, Céline, Boyer, Olivier, Joly, Pascal, Calbo, Sébastien, Riou, Gaetan, Derambure, Céline, Physiopathologie, Autoimmunité, maladies Neuromusculaires et THErapies Régénératrices (PANTHER), Université de Rouen Normandie (UNIROUEN), Normandie Université (NU)-Normandie Université (NU)-Institut National de la Santé et de la Recherche Médicale (INSERM), and Génomique et Médecine Personnalisée du Cancer et des Maladies Neuropsychiatriques (GPMCND)

Subjects
rituximab, autoreactive B cell, [SDV.IMM] Life Sciences [q-bio]/Immunology, [SDV.BBM.GTP]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Genomics [q-bio.GN], MESH: autoreactive B cell, corticosteriods, pemphigus, transcriptomic analysis, [SDV.IMM]Life Sciences [q-bio]/Immunology, [SDV.BBM.GTP] Life Sciences [q-bio]/Biochemistry, Molecular Biology/Genomics [q-bio.GN]
Abstract

International audience; Pemphigus Vulgaris is an autoimmune disease of the skin and mucous membranes, which is due to the production of pathogenic autoantibodies targeting desmoglein (DSG) 1 and 3, which are adhesion proteins of the keratinocytes. Rituximab is an anti-CD20 mAb which induces a prolonged depletion of blood B cells. We recently showed that rituximab was more effective than a standard oral corticosteroid (CS) treatment, allowing 90% of patients to achieve complete remission (CR). Additionally, we showed that DSG-specific-B (DSG positive) cells were still detectable during the B cell recovery which follows the initial rituximab-induced B cell depletion, even in patients in CR. In order to characterize DSG positive B cells in patients in CR after rituximab or CS treatment relative to those detectable at baseline in patients with an active pemphigus, we studied the expression profile of 31 genes of interest related to inflammatory cytokines, TNF receptors and activation markers. Using quantitative Polymerase Chain Reaction performed on one cell with a microfluidic technique, we found that patients' autoreactive B cells collected at baseline had a significantly higher expression of genes encoding for IL-1β, IL-23p19, and IL-12p35 pro-inflammatory cytokines and the IRF5 transcription factor, than non-autoreactive B cells. Surprisingly, the gene expression profile of DSG positive B cells collected after rituximab treatment in patients in CR was close to that of DSG positive B cells at baseline in patients with active pemphigus, except for the IL-1β and the CD27 memory marker genes, which were under-expressed after rituximab compared to baseline. Conversely, we observed a decreased expression of genes encoding for IL-1β and IL-23p19 in patients treated with CS relative to baseline. This study showed that: (i) DSG positive autoreactive B cells have a different gene expression profile than non-autoreactive B cells; (ii) rituximab and CS have different effects on the genes' expression in autoreactive DSG positive B cells from pemphigus patients.

Published
2019

14. New Insights into Plant Extracellular DNA. A Study in Soybean Root Extracellular Trap

Author

Chambard, Marie, primary, Plasson, Carole, additional, Derambure, Céline, additional, Coutant, Sophie, additional, Tournier, Isabelle, additional, Lefranc, Benjamin, additional, Leprince, Jérôme, additional, Kiefer-Meyer, Marie-Christine, additional, Driouich, Azeddine, additional, Follet-Gueye, Marie-Laure, additional, and Boulogne, Isabelle, additional

Published
2021
Full Text
View/download PDF

15. Additional file 1 of Assessment of branch point prediction tools to predict physiological branch points and their alteration by variants

Author

Leman, Raphaël, Tubeuf, Hélène, Raad, Sabine, Tournier, Isabelle, Derambure, Céline, Lanos, Raphaël, Gaildrat, Pascaline, Castelain, Gaia, Hauchard, Julie, Killian, Audrey, Baert-Desurmont, Stéphanie, Legros, Angelina, Goardon, Nicolas, Quesnelle, Céline, Ricou, Agathe, Castera, Laurent, Vaur, Dominique, Gac, Gérald, Ka, Chandran, Fichou, Yann, Bonnet-Dorion, Françoise, Sevenet, Nicolas, Guillaud-Bataille, Marine, Boutry-Kryza, Nadia, Schultz, Inès, Caux-Moncoutier, Virginie, Rossing, Maria, Walker, Logan, Spurdle, Amanda, Houdayer, Claude, Martins, Alexandra, and Krieger, Sophie

Abstract

Additional file 1: Figure S1. Workflow to compare bioinformatics tools on Ensembl and RNA-seq data for the predictions of branch point (BP). Figure S2. The different ways that a variant may alter the branch point score. Figure S3. Running time of the four tools SVM-BPfinder, BPP, Branchpointer, and LaBranchor. Figure S4. Paired comparison of the five tools from the Ensembl data and from the RNA-seq data. Figure S5. The overlap of natural 3′ ss (True Calls) and controls AG (False Calls) from Ensembl data. Figure S6. Splicing junctions filtered out from RNA-seq data. Alt 3’ss: alternative acceptor splice sites. Figure S7. The distribution of the relative expression of alternative 3’ss. Figure S8. The overlap of alternative 3′ ss (True Calls) and controls AG (False Calls) from our RNAseq data. Figure S9. Correlation between the scores (SVM-BPfinder, BPP, Branchpointer, LaBranchoR, RNABPS) and the expression of alternative 3’ss. Figure S10. Repartition of variants (n = 120) according their position relative to the predicted branch point. Figure S11:. Determination of optimal motif (YTRAYNN) length to predict splicing alteration, n = 120 variants. ACC: Accuracy, Pos: relative position in branch point motif, Se: Sensitivity, Sp: Specificity. Figure S12. Cross-validation (1000 times) to select the optimal model to predict branch point alteration. Figure S13. Cross-validation (1000 times) to select the optimal model to predict branch point alteration without the positions of predicted BP for all tools except BPP.

Published
2020
Full Text
View/download PDF

22. Assessment of branch point prediction tools to predict physiological branch points and their alteration by variants

Author

Leman, Raphael, primary, Tubeuf, Hélène, additional, Raad, Sabine, additional, Tournier, Isabelle, additional, Derambure, Céline, additional, Lanos, Raphaël, additional, Gaildrat, Pascaline, additional, Castelain, Gaia, additional, Hauchard, Julie, additional, Killian, Audrey, additional, Baert-Desurmont, Stéphanie, additional, Legros, Angelina, additional, Goardon, Nicolas, additional, Quesnelle, Céline, additional, Ricou, Agathe, additional, Castera, Laurent, additional, Vaur, Dominique, additional, Gac, Gérald Le, additional, Ka, Chandran, additional, Fichou, Yann, additional, Bonnet-Dorion, Françoise, additional, Sevenet, Nicolas, additional, Guillaud-Bataille, Marine, additional, Boutry-Kryza, Nadia, additional, Schultz, Ines, additional, Caux-Moncoutier, Virginie, additional, Rossing, Maria, additional, Walker, Logan C, additional, Spurdle, Amanda B, additional, Houdayer, Claude, additional, Martins, Alexandra, additional, and Krieger, Sophie, additional

Published
2020
Full Text
View/download PDF

24. Assessment of branch point prediction tools to predict physiological branch points and their alteration by variants

Author

Leman, Raphael, primary, Tubeuf, Hélène, additional, Raad, Sabine, additional, Tournier, Isabelle, additional, Derambure, Céline, additional, Lanos, Raphaël, additional, Gaildrat, Pascaline, additional, Castelain, Gaia, additional, Hauchard, Julie, additional, Killian, Audrey, additional, Baert-Desurmont, Stéphanie, additional, Legros, Angelina, additional, Goardon, Nicolas, additional, Quesnelle, Céline, additional, Ricou, Agathe, additional, Castera, Laurent, additional, Vaur, Dominique, additional, Gac, Gérald Le, additional, Ka, Chandran, additional, Fichou, Yann, additional, Bonnet-Dorion, Françoise, additional, Sevenet, Nicolas, additional, Guillaud-Bataille, Marine, additional, Boutry-Kryza, Nadia, additional, Schultz, Ines, additional, Caux-Moncoutier, Virginie, additional, Rossing, Maria, additional, Walker, Logan C, additional, Spurdle, Amanda B, additional, Houdayer, Claude, additional, Martins, Alexandra, additional, and Krieger, Sophie, additional

Published
2019
Full Text
View/download PDF

26. Blood functional assay for rapid clinical interpretation of germline TP53 variants.

Author

Raad, Sabine, Rolain, Marion, Coutant, Sophie, Derambure, Céline, Lanos, Raphael, Charbonnier, Françoise, Bou, Jacqueline, Bouvignies, Emilie, Lienard, Gwendoline, Vasseur, Stéphanie, Farrel, Michael, Ingster, Olivier, Desurmont, Stéphanie Baert, Kasper, Edwige, Bougeard, Gaëlle, Frébourg, Thierry, and Tournier, Isabelle

Abstract

Background The interpretation of germline TP53 variants is critical to ensure appropriate medical management of patients with cancer and follow-up of variant carriers. This interpretation remains complex and is becoming a growing challenge considering the exponential increase in TP53 tests. We developed a functional assay directly performed on patients' blood. Methods Peripheral blood mononuclear cells were cultured, activated, exposed to doxorubicin and the p53-mediated transcriptional response was quantified using reverse transcription-multiplex ligation probe amplification and RT-QMPSF assays, including 10 p53 targets selected from transcriptome analysis, and two amplicons to measure p53 mRNA levels. We applied this blood functional assay to 77 patients addressed for TP53 analysis. Results In 51 wild-type TP53 individuals, the mean p53 functionality score was 12.7 (range 7.5-22.8). Among eight individuals harbouring likely pathogenic or pathogenic variants, the scores were reduced (mean 4.8, range 3.1-7.1), and p53 mRNA levels were reduced in patients harbouring truncating variants. We tested 14 rare unclassified variants (p.(Pro72His), p.(Gly105Asp), p.(Arg110His), p.(Phe134Leu), p.(Arg158Cys), p.(Pro191Arg), p.(Pro278Arg), p.(Arg283Cys), p.(Leu348Ser), p.(Asp352Tyr), p.(Gly108_ Phe109delinsVal), p.(Asn131del), p.(Leu265del), c.-117G>T) and 12 yielded functionally abnormal scores. Remarkably, the assay revealed that the c.*1175A>C polymorphic variant within TP53 poly-adenylation site can impact p53 function with the same magnitude as a null variant, when present on both alleles, and may act as a modifying factor in pathogenic variant carriers. Conclusion This blood p53 assay should therefore be a useful tool for the rapid clinical classification of germline TP53 variants and detection of non-coding functional variants. [ABSTRACT FROM AUTHOR]

Published
2021
Full Text
View/download PDF

32. Characterization of CD11c+ B-cells from human blood

Author

Calbo, Sébastien F, primary, Demeules, Mélanie, additional, Derambure, Céline, additional, Riou, Gaetan, additional, Vaillant, Maud, additional, Boyer, Olivier, additional, Joly, Pascal, additional, and Golinski, Marie-Laure, additional

Published
2019
Full Text
View/download PDF

33. Proteomic and transcriptomic study of brain microvessels in neonatal and adult mice

Author

Porte, Baptiste, Chatelain, Clémence, Hardouin, Julie, Derambure, Céline, Zerdoumi, Yasmine, Hauchecorne, Michèle, Dupré, Nicolas, Bekri, Soumeya, Gonzalez, Bruno, Marret, Stéphane, Cosette, Pascal, Leroux, Philippe, Génomique et Médecine Personnalisée du Cancer et des Maladies Neuropsychiatriques (GPMCND), Université de Rouen Normandie (UNIROUEN), Normandie Université (NU)-Normandie Université (NU)-Institut National de la Santé et de la Recherche Médicale (INSERM), Team 4 'NeoVasc' - INSERM U1245, Normandie Université (NU)-Normandie Université (NU)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Rouen Normandie (UNIROUEN), Polymères Biopolymères Surfaces (PBS), Institut national des sciences appliquées Rouen Normandie (INSA Rouen Normandie), Institut National des Sciences Appliquées (INSA)-Normandie Université (NU)-Institut National des Sciences Appliquées (INSA)-Normandie Université (NU)-Institut de Chimie du CNRS (INC)-Institut Normand de Chimie Moléculaire Médicinale et Macromoléculaire (INC3M), Institut de Chimie du CNRS (INC)-École Nationale Supérieure d'Ingénieurs de Caen (ENSICAEN), Normandie Université (NU)-Normandie Université (NU)-Institut national des sciences appliquées Rouen Normandie (INSA Rouen Normandie), Institut National des Sciences Appliquées (INSA)-Normandie Université (NU)-Institut National des Sciences Appliquées (INSA)-Université Le Havre Normandie (ULH), Normandie Université (NU)-Université de Rouen Normandie (UNIROUEN), Normandie Université (NU)-Centre National de la Recherche Scientifique (CNRS)-Université de Caen Normandie (UNICAEN), Normandie Université (NU)-École Nationale Supérieure d'Ingénieurs de Caen (ENSICAEN), Normandie Université (NU)-Université Le Havre Normandie (ULH), Normandie Université (NU)-Centre National de la Recherche Scientifique (CNRS), Plate-forme de Protéomique PISSARO, Institute for Research and Innovation in Biomedicine (IRIB), Normandie Université (NU)-Normandie Université (NU)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Université de Rouen Normandie (UNIROUEN), Normandie Université (NU)-Normandie Université (NU)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Laboratoire de biochimie générale [Rouen], CHU Rouen, Normandie Université (NU)-Normandie Université (NU)-Université de Rouen Normandie (UNIROUEN), Normandie Université (NU)-Centre hospitalier universitaire de Rouen, Service de pédiatrie néonatale et réanimation - neuropédiatrie [CHU Rouen], Hôpital Charles Nicolle [Rouen]-CHU Rouen, Normandie Université (NU), Normandie Université (NU)-Normandie Université (NU)-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Rouen Normandie (UNIROUEN), Normandie Université (NU)-Normandie Université (NU)-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM), and Normandie Université (NU)-Normandie Université (NU)-Hôpital Charles Nicolle [Rouen]-Université de Rouen Normandie (UNIROUEN)

Subjects
Metabolic Processes, Proteomics, Aging, Proteome, Physiology, lcsh:Medicine, Hemorrhage, Bioenergetics, Pathology and Laboratory Medicine, Biochemistry, Vascular Medicine, Antioxidants, Signs and Symptoms, Diagnostic Medicine, [SDV.BBM.GTP]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Genomics [q-bio.GN], Medicine and Health Sciences, Genetics, Animals, lcsh:Science, Energy-Producing Organelles, Protein Metabolism, Gene Expression Profiling, lcsh:R, Biology and Life Sciences, Computational Biology, Brain, Genomics, Cell Biology, Genome Analysis, Mitochondria, Mice, Inbred C57BL, Metabolism, Gene Ontology, [SDV.BDD.EO]Life Sciences [q-bio]/Development Biology/Embryology and Organogenesis, Animals, Newborn, Gene Expression Regulation, Microvessels, lcsh:Q, Metabolic Pathways, Cellular Structures and Organelles, Energy Metabolism, Physiological Processes, Transcriptome Analysis, Glycolysis, Research Article
Abstract

International audience; Infants born before 29 weeks gestation incur a major risk of preterm encephalopathy and subependymal/intracerebral/intraventricular haemorrhage. In mice, an ontogenic window of haemorrhage risk was recorded up to 5 days after birth in serpine1 knockout animals. Using proteome and transcriptome approaches in mouse forebrain microvessels, we previously described the remodelling of extracellular matrix and integrins likely strengthening the vascular wall between postnatal day 5 (P5) and P10. Haemorrhage is the ultimate outcome of vessel damage (i.e., during ischaemia), although discreet vessel insults may be involved in the aetiology of preterm encephalopathy. In this study, we examined proteins identified by mass spectrometry and segregating in gene ontology pathways in forebrain microvessels in P5, P10, and adult wild type mice. In parallel, comparative transcript levels were obtained using RNA hybridization microarrays and enriched biological pathways were extracted from genes exhibiting at least a twofold change in expression. Five major biological functions were observed in those genes detected both as proteins and mRNA expression undergoing at least a twofold change in expression in one or more age comparisons: energy metabolism , protein metabolism, antioxidant function, ion exchanges, and transport. Adult micro-vessels exhibited the highest protein and mRNA expression levels for a majority of genes. Energy metabolism-enriched gene ontology pathways pointed to the preferential occurrence of glycolysis in P5 microvessels cells versus P10 and adult preparations enriched in aerobic oxidative enzymes. Age-dependent levels of RNA coding transport proteins at the plasma membrane and mitochondria strengthened our findings based on protein data. The data suggest that immature microvessels have fewer energy supply alternatives to glycoly-sis than mature structures. In the context of high energy demand, this constraint might account for vascular damage and maintenance of the high bleeding occurrence in specific areas in immature brain.

Published
2017

34. Additional file 1: Table S1. of Dysregulation of RasGRP1 in rheumatoid arthritis and modulation of RasGRP3 as a biomarker of TNFα inhibitors

Author

Marie-Laure Golinski, Vandhuick, Thibault, Derambure, Céline, Fréret, Manuel, Lecuyer, Matthieu, Guillou, Clément, Hiron, Martine, Boyer, Olivier, Loët, Xavier Le, Vittecoq, Olivier, and Lequerré, Thierry

Subjects
musculoskeletal diseases, skin and connective tissue diseases, humanities
Abstract

Clinical and biological characteristics of rheumatoid arthritis (RA) patients treated with adalimumab, etanercept or abatacept. (DOC 61 kb)

Published
2015
Full Text
View/download PDF

36. Major remodeling of brain microvessels during neonatal period in the mouse: A proteomic and transcriptomic study

Author

Porte, Baptiste, primary, Hardouin, Julie, additional, Zerdoumi, Yasmine, additional, Derambure, Céline, additional, Hauchecorne, Michèle, additional, Dupre, Nicolas, additional, Obry, Antoine, additional, Lequerre, Thierry, additional, Bekri, Soumeya, additional, Gonzalez, Bruno, additional, Flaman, Jean M, additional, Marret, Stéphane, additional, Cosette, Pascal, additional, and Leroux, Philippe, additional

Published
2016
Full Text
View/download PDF

37. Soluble alpha-enolase activates monocytes by CD14-dependent TLR4 signalling pathway and exhibits a dual function

Author

Guillou, Clément, primary, Fréret, Manuel, additional, Fondard, Emeline, additional, Derambure, Céline, additional, Avenel, Gilles, additional, Golinski, Marie-Laure, additional, Verdet, Mathieu, additional, Boyer, Olivier, additional, Caillot, Frédérique, additional, Musette, Philippe, additional, Lequerré, Thierry, additional, and Vittecoq, Olivier, additional

Published
2016
Full Text
View/download PDF

38. Dysregulation of RasGRP1 in rheumatoid arthritis and modulation of RasGRP3 as a biomarker of TNFα inhibitors

Author

Golinski, Marie-Laure, primary, Vandhuick, Thibault, additional, Derambure, Céline, additional, Fréret, Manuel, additional, Lecuyer, Matthieu, additional, Guillou, Clément, additional, Hiron, Martine, additional, Boyer, Olivier, additional, Le Loët, Xavier, additional, Vittecoq, Olivier, additional, and Lequerré, Thierry, additional

Published
2015
Full Text
View/download PDF

39. Prophylactic Injection of Recombinant Alpha-Enolase Reduces Arthritis Severity in the Collagen-Induced Arthritis Mice Model

Author

Guillou, Clément, primary, Derambure, Céline, additional, Fréret, Manuel, additional, Verdet, Mathieu, additional, Avenel, Gilles, additional, Golinski, Marie-Laure, additional, Sabourin, Jean-Christophe, additional, Loarer, François Le, additional, Adriouch, Sahil, additional, Boyer, Olivier, additional, Lequerré, Thierry, additional, and Vittecoq, Olivier, additional

Published
2015
Full Text
View/download PDF

43. Early and long-standing rheumatoid arthritis: distinct molecular signatures identified by gene-expression profiling in synovia

Author

Lequerré, Thierry, primary, Bansard, Carine, additional, Vittecoq, Olivier, additional, Derambure, Céline, additional, Hiron, Martine, additional, Daveau, Maryvonne, additional, Tron, François, additional, Ayral, Xavier, additional, Biga, Norman, additional, Auquit-Auckbur, Isabelle, additional, Chiocchia, Gilles, additional, Le Loët, Xavier, additional, and Salier, Jean-Philippe, additional

Published
2009
Full Text
View/download PDF

44. Gene profiling in white blood cells predicts infliximab responsiveness in rheumatoid arthritis

Author

Lequerré, Thierry, primary, Gauthier-Jauneau, Anne-Christine, additional, Bansard, Carine, additional, Derambure, Céline, additional, Hiron, Martine, additional, Vittecoq, Olivier, additional, Daveau, Maryvonne, additional, Mejjad, Othmane, additional, Daragon, Alain, additional, Tron, François, additional, Le Loët, Xavier, additional, and Salier, Jean-Philippe, additional

Published
2006
Full Text
View/download PDF

48. Long-range identification of hepatocyte nuclear factor-3 (FoxA) high and low-affinity binding sites with a chimeric nuclease11Edited by M. Yaniv

Author

Ruminy, Philippe, Derambure, Céline, Chandrasegaran, Srinivasan, and Salier, Jean-Philippe

Abstract

Identifying the complete set of transcription factors that bind the promoter and other regulatory regions of a gene of interest is an essential step in functional genomics. We have developed an original assay for the systematic detection of hepatocyte nuclear factor-3 (HNF-3) binding sites within cloned promoters. This assay is based on expression of a recombinant enzyme, HNF-3β/FN, that is comprised of the rat HNF-3β DNA-binding domain and the non-specific nuclease domain of the FokI restriction enzyme. Southern analysis of target plasmids with proven HNF-3 binding sites showed that HNF-3β/FNwas able to specifically cut both DNA strands in the vicinity of these binding sites, whereas mutagenized binding sites were no longer cleaved. Likewise, as yet undescribed HNF-3 binding sites were detected easily over a distance spanning several thousand bases. The functionality of such binding sites was confirmed by electromobility shift assay. Furthermore, the extent of cleavage by HNF-3β/FNat a given binding site was tightly correlated with the affinity of a natural HNF-3β molecule for this site. This novel approach can be extended to other transcription factors for long-range identification of functional transcription factor binding sites in genes.

Published
2001
Full Text
View/download PDF

49. A new approach in rheumatology: large scale analysis of gene expression⋄<fn id="FN1"><no>1</no>Pour citer cet article, utiliser ce titre en anglais, re´fe´rence parue dans Joint Bone Spine, 2003, vol. 70.</fn>

Author

Lequerré, Thierry, Coulouarn, Cédric, Derambure, Céline, Lefebvre, Grégory, Vittecoq, Olivier, Daveau, Maryvonne, Salier, Jean-Philippe, and Le Loët, Xavier

Subjects
*GENE expression, *DNA, *RHEUMATOLOGY, *DNA microarrays, *MESSENGER RNA
Abstract

Large-scale analysis of gene expression with cDNA arrays is spreading over many biological fields, including rheumatology. In this report, we wish to explain the principle and main advantages of this tool in the context of our discipline. Until 1995, analysis of gene expression was conducted for a few genes at a time but DNA chips now allow one to monitor the expression of thousands of genes in a single experiment and analyze the transcriptome, i.e. the whole of the transcripts in a given cell or tissue. Whatever the platform used (macro- or microarrays, oligo-chips), this technology rests upon the hybridization of i) a set of cDNA clones tethered to a solid support (nylon or glass) as probes, and ii) labelled cDNAs that are reverse-transcribed from bulk mRNAs extracted from a cell or tissue sample as a target. The end result is information on the relative abundance of every mRNA between two or more samples. The transcriptome analysis has two main objectives in rheumatology: i) identifying a gene expression profile that is a hallmark of a pathology and using it for a diagnostic or prognostic purpose, and ii) gathering genes with similar changes of expression, which allows one to specify the identity of novel proteins involved in a well-known intracellular cascade of regulation or even to identify new cascades. [Copyright &y& Elsevier]

Published
2003
Full Text
View/download PDF

50. CD11c + B Cells Are Mainly Memory Cells, Precursors of Antibody Secreting Cells in Healthy Donors.

Author

Golinski ML, Demeules M, Derambure C, Riou G, Maho-Vaillant M, Boyer O, Joly P, and Calbo S

Subjects
Adult, Aged, Antigen Presentation, Cells, Cultured, Female, Healthy Volunteers, Humans, Lymphocyte Activation immunology, Male, Middle Aged, Pemphigus immunology, Phenotype, Young Adult, CD11c Antigen metabolism, Cell Differentiation immunology, Immunologic Memory, Plasma Cells immunology, Receptors, Antigen, B-Cell metabolism
Abstract

CD11c + B cells have been reported to be increased in autoimmune diseases, but they are detected in the blood of healthy individuals as well. We aimed to characterize CD11c + B cells from healthy donors by flow cytometry, microarray analysis, and in vitro functional assays. Here, we report that CD11c + B cells are a distinct subpopulation of B cells, enriched in the memory subpopulation even if their phenotype is heterogeneous, with overexpression of genes involved in B-cell activation and differentiation as well as in antigen presentation. Upon activation, CD11c + B cells can differentiate into antibody-secreting cells, and CD11c could be upregulated in CD11c - B cells by B-cell receptor activation. Finally, we show that patients with pemphigus, an autoimmune disease mediated by B cells, have a decreased frequency of CD11c + B cell after treatment, relative to baseline. Our findings show that CD11c + B cells are mainly memory B cells prone to differentiate into antibody secreting cells that accumulate with age, independently of gender., (Copyright © 2020 Golinski, Demeules, Derambure, Riou, Maho-Vaillant, Boyer, Joly and Calbo.)

Published
2020
Full Text
View/download PDF

Catalog

Books, media, physical & digital resources
See catalog results