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Your search keyword '"Desrames, Alexandra"' showing total 17 results
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17 results on '"Desrames, Alexandra"'

1. An ACE2-Based Bimodular Fusion Protein Enables Reorientation of Endogenous Anti–Epstein-Barr Virus Antibodies Toward SARS-CoV-2 Spike.

Author

Chêne, Arnaud, Desrames, Alexandra, Tomlinson, Alice, Ruffié, Claude, Tangy, Frédéric, and Gamain, Benoît

Subjects
*VIRAL antibodies, *CHIMERIC proteins, *COVID-19, *SARS-CoV-2, *ANGIOTENSIN converting enzyme
Abstract

The use of soluble recombinant angiotensin-converting enzyme 2 (rACE2) as a decoy capable of blocking SARS-CoV-2 entry into cells has been envisaged as a therapeutic strategy to reduce viral loads in patients with severe COVID-19. We engineered a novel form of rACE2, fused to the Epstein-Barr virus antigen P18F3 (rACE2-P18F3), to reorient a preexisting humoral response toward Epstein-Barr virus against SARS-CoV-2 particles. Recombinant ACE2-P18F3 was able to bind to the SARS-CoV-2 spike protein, neutralize viral entry into cells, and promote the phagocytosis of spheres coated with different spike variants by monocytic cells. The results position rACE2-P18F3 as a promising therapeutic candidate to universally block coronavirus cell entry and clear viral particles. [ABSTRACT FROM AUTHOR]

Published
2023
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3. BMFPs, a versatile therapeutic tool for redirecting a preexisting Epstein-Barr virus antibody response toward defined target cells

Author

Gamain, Benoît, primary, Brousse, Carine, additional, Rainey, Nathan E., additional, Diallo, Béré K., additional, Paquereau, Clara-Eva, additional, Desrames, Alexandra, additional, Ceputyte, Jolita, additional, Semblat, Jean-Philippe, additional, Bertrand, Olivier, additional, Gangnard, Stéphane, additional, Teillaud, Jean-Luc, additional, and Chêne, Arnaud, additional

Published
2022
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4. HIV-1 hijacks the cell extracellular matrix to spread collectively and efficiently between T lymphocytes

Author

Inizan, Catherine, primary, Caillet, Marina, additional, Desrames, Alexandra, additional, Bomme, Perrine, additional, MALLET, Adeline, additional, Bourdic, Katia, additional, Lambotte, Olivier, additional, Boufassa, Faroudy, additional, Mesel-Lemoine, Mariana, additional, Tangy, Frederic, additional, Pancino, Gianfranco, additional, Mouquet, Hugo, additional, Lagache, thibault, additional, Dufour, Alexandre C, additional, Olivo-Marin, Jean-Christophe, additional, Rey, Felix A, additional, Saez-Cirion, Asier, additional, and Thoulouze, Maria-Isabel, additional

Published
2021
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5. Bi-modular fusion proteins, a versatile therapeutic tool for re-directing a pre-existing Epstein-Barr virus antibody response towards defined target cells

Author

Gamain, Benoît, primary, Brousse, Carine, additional, Rainey, Nathan, additional, Diallo, Béré, additional, Paquereau, Clara-Eva, additional, Desrames, Alexandra, additional, Ceputyte, Jolita, additional, Semblat, Jean-Philippe, additional, Bertrand, Olivier, additional, Gangnard, Stéphane, additional, Teillaud, Jean-Luc, additional, and CHENE, Arnaud, additional

Published
2021
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7. Immunohistochemical and virological features of HTLV-1-associated myosites: a study of 13 patients from West Indies and Africa

Author

Martin-Latil Sandra, Bassot Sylviane, Khun Huot, Cumont Marie-Christine, Huerre Michel, Wattel Eric, Mortreux Franck, Morin Anne-Sophie, Texeira Antonio, Chérin Patrick, Haroche Julien, Amoura Zahir, Lacour Arnaud, Papo Thomas, Marcorelles Pascale, Zagnoli Fabien, Penisson-Besnier Isabelle, Mikol Jacqueline, Polivka Marc, Hermine Olivier, Aouba Achille, Desrames Alexandra, Maisonobe Thierry, Cassar Olivier, Desdouits Marion, Taylor Graham, Gessain Antoine, Ozden Simona, and Ceccaldi Pierre-Emmanuel

Subjects
Immunologic diseases. Allergy, RC581-607
Published
2011
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10. Multiple recombinant events in human T-cell Leukemia virus Type 1: complete sequences of recombinant African strains

Author

Cassar, Olivier, primary, Desrames, Alexandra, additional, Marçais, Ambroise, additional, Gout, Olivier, additional, Taylor, Graham P., additional, Hermine, Olivier, additional, Soriano, Vicente, additional, Mendoza, Carmen de, additional, Dehan, Océane, additional, Mener, Margot Le, additional, Afonso, Philippe V., additional, and Gessain, Antoine, additional

Published
2020
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11. Stomatin modulates the activity of the Anion Exchanger 1 (AE1, SLC4A1)

Author

Genetet, Sandrine, Desrames, Alexandra, Chouali, Youcef, Ripoche, Pierre, Lopez, Claude, and Mouro-Chanteloup, Isabelle

Subjects
Erythrocytes, Membrane Proteins, Models, Biological, Article, Cell Line, stomatognathic diseases, Bicarbonates, Gene Knockout Techniques, HEK293 Cells, stomatognathic system, Chlorides, Anion Exchange Protein 1, Erythrocyte, Humans, Protein Binding
Abstract

Anion Exchanger 1 (AE1) and stomatin are integral proteins of the red blood cell (RBC) membrane. Erythroid and kidney AE1 play a major role in HCO3− and Cl− exchange. Stomatins down-regulate the activity of many channels and transporters. Biochemical studies suggested an interaction of erythroid AE1 with stomatin. Moreover, we previously reported normal AE1 expression level in stomatin-deficient RBCs. Here, the ability of stomatin to modulate AE1-dependent Cl−/HCO3− exchange was evaluated using stopped-flow methods. In HEK293 cells expressing recombinant AE1 and stomatin, the permeabilities associated with AE1 activity were 30% higher in cells overexpressing stomatin, compared to cells with only endogenous stomatin expression. Ghosts from stomatin-deficient RBCs and controls were resealed in the presence of pH- or chloride-sensitive fluorescent probes and submitted to inward HCO3− and outward Cl− gradients. From alkalinization rate constants, we deduced a 47% decreased permeability to HCO3− for stomatin-deficient patients. Similarly, kinetics of Cl− efflux, followed by the probe dequenching, revealed a significant 42% decrease in patients. In situ Proximity Ligation Assays confirmed an interaction of AE1 with stomatin, in both HEK recombinant cells and RBCs. Here we show that stomatin modulates the transport activity of AE1 through a direct protein-protein interaction.

Published
2017
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12. Adult T-Cell Leukemia/Lymphoma in a Caucasian Patient After Sexual Transmission of Human T-Cell Lymphotropic Virus Type 1

Author

Sibon, David, primary, Cassar, Olivier, additional, Duga, Isabelle, additional, Brouzes, Chantal, additional, Ghez, David, additional, Pasquier, Christophe, additional, Sibon, Claire, additional, Desrames, Alexandra, additional, Mortreux, Franck, additional, Wattel, Eric, additional, Bazarbachi, Ali, additional, Gessain, Antoine, additional, and Hermine, Olivier, additional

Published
2015
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14. HTLV-1-associated inflammatory myopathies: Low proviral load and moderate inflammation in 13 patients from West Indies and West Africa

Author

Desdouits, Marion, primary, Cassar, Olivier, additional, Maisonobe, Thierry, additional, Desrames, Alexandra, additional, Aouba, Achille, additional, Hermine, Olivier, additional, Mikol, Jacqueline, additional, Polivka, Marc, additional, Penisson-Besnier, Isabelle, additional, Marcorelles, Pascale, additional, Zagnoli, Fabien, additional, Papo, Thomas, additional, Lacour, Arnaud, additional, Amoura, Zahir, additional, Haroche, Julien, additional, Cherin, Patrick, additional, Teixeira, Antonio, additional, Benveniste, Olivier, additional, Herson, Serge, additional, Morin, Anne-Sophie, additional, Mortreux, Franck, additional, Wattel, Eric, additional, Huerre, Michel, additional, Cumont, Marie-Christine, additional, Martin-Latil, Sandra, additional, Butler-Browne, Gillian, additional, Gout, Olivier, additional, Taylor, Graham, additional, Gessain, Antoine, additional, Ozden, Simona, additional, and Ceccaldi, Pierre-Emmanuel, additional

Published
2013
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15. Immunohistochemical and virological features of HTLV-1-associated myosites: a study of 13 patients from West Indies and Africa

Author

Desdouits, Marion, primary, Cassar, Olivier, additional, Maisonobe, Thierry, additional, Desrames, Alexandra, additional, Aouba, Achille, additional, Hermine, Olivier, additional, Mikol, Jacqueline, additional, Polivka, Marc, additional, Penisson-Besnier, Isabelle, additional, Marcorelles, Pascale, additional, Zagnoli, Fabien, additional, Papo, Thomas, additional, Lacour, Arnaud, additional, Amoura, Zahir, additional, Haroche, Julien, additional, Chérin, Patrick, additional, Texeira, Antonio, additional, Morin, Anne-Sophie, additional, Mortreux, Franck, additional, Wattel, Eric, additional, Huerre, Michel, additional, Cumont, Marie-Christine, additional, Khun, Huot, additional, Bassot, Sylviane, additional, Martin-Latil, Sandra, additional, Taylor, Graham, additional, Gessain, Antoine, additional, Ozden, Simona, additional, and Ceccaldi, Pierre-Emmanuel, additional

Published
2011
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17. Expression and Purification of scFv 2H7 -P18F3, a Bi-Modular Fusion Protein (BMFP) Targeting Human CD20.

Author

Brousse C, Rainey NE, Desrames A, Teillaud JL, Gamain B, and Chêne A

Abstract

P18F3-based bi-modular fusion proteins (BMFPs), designed to re-direct pre-existing anti-Epstein-Barr virus (EBV) endogenous polyclonal antibodies towards defined target cells, demonstrated efficient biological activity in a mouse tumor model and could potentially represent a universal and versatile platform to develop novel therapeutics against a broad range of diseases. This protocol provides step-by-step instructions for expressing scFv 2H7 -P18F3, a BMFP targeting human CD20, in Escherichia coli (SHuffle ® ), and for purifying soluble proteins using a two-step process, namely immobilized metal affinity chromatography (IMAC) followed by size exclusion chromatography. This protocol can also be used for expression and purification of other BMFPs with alternative binding specificities., Competing Interests: Competing interestsBG and AC are inventors on a patent application related to this protocol filed by Institut National de la Transfusion Sanguine, Centre National de la Recherche Scientifique (CNRS), Inserm, Université de Paris (now universtité Paris Cité) (no. WO2017103020A1, filed 25 December 2015, published 22 June 2017). The authors declare that they have no other competing interests., (Copyright © 2023 The Authors; exclusive licensee Bio-protocol LLC.)

Published
2023
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