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The increasing use of automation in fishing vessels has improved trawling efficiency while directly affecting the fishing capacity and cost of fishing vessels. Among the various influencing factors, warp tension and warp length can be varied to automatically balance the retraction and release of warp control. We combined the two parameters and independently designed and developed the key equipment for fishing vessels—the warp dynamometer and meter counter—and control software. The accuracy of the warp tension and length measurements was improved. The designed equipment was applied to sea trials under different working conditions, and the test data records were exported. Next, filtered time-domain graphs of the required parameters were plotted through complex Fourier transform, first-order low-pass filtering, and inverse Fourier transform. The results of data processing using various parameters were compared and analysed to determine the variation trends of the parameters and verify the effects of their balance control. The results indicated that using an automatic balance control system that combines warp tension and warp length can be effective for the fishing operation of offshore double-deck trawlers. In addition, first-order low-pass filtering can be used to filter complex warp tension data. This study also determined the relationship between warp tension and experimental parameters such as warp length and ship speed during the release of control. After the balance control of warp tension and warp length, the net mouth area increased by 30.7% and 36.5%, respectively, and the fishing efficiency of the vessel improved considerably.

The oscillating buoy wave energy converter (OBWEC) captures wave energy through the undulating movement of the buoy in the waves. In the process of capturing wave energy, the hydrodynamic performance of the buoy plays an important role. This paper designed the “Haida No. 1” OBWEC, in which the buoy adopts a form of swinging motion. In order to further improve the hydrodynamic performance of the buoy, a 2D numerical wave tank (NWT) model is established using ADINA software based on the working principle of the device. According to the motion equation of the buoy in the waves, the influence of the buoy shape, arm length, tilt angle, buoy draft, buoy width, wave height and Power Take-off (PTO) damping on the hydrodynamic performance of the buoy is studied. Finally, a series of physical experiments are performed on the device in a laboratory pool. The experimental results verify the consistency of the numerical results. The research results indicate that the energy conversion efficiency of the device can be improved by optimizing the hydrodynamic performance of the buoy. However, the absorption efficiency of a single buoy for wave energy is limited, so it is very difficult to achieve full absorption of wave energy.

Allergic rhinitis (AR) is an IgE-mediated chronic inflammatory disease of the allergic nasal mucosa. It has a significant effect on quality life; most patients with AR also suffer from sleep disorders, mood disorders, and deterioration in social relationships. As increasing numbers of medicinal plants show productive anti-inflammatory activity against inflammatory diseases, there is growing interest in natural medicinal plant ingredients. To this end, we selected Astragalus polysaccharides (APS) to evaluate its anti-inflammatory effect on ovalbumin-induced AR rats, and we further explored its impact on NLRP3 inflammasome activation and NOD2-mediated NF-κB activation. We found that APS can alleviate the nasal symptom of AR rats and attenuate pathological alterations. APS also reduced the inflammatory cytokine levels. APS not only inhibited the NLRP3 inflammasome activation but also inhibited NF-κB activation by decreasing NOD2 expression and blocking the phosphorylation of NF-κB (p65). In conclusion, APS can effectively improve the inflammatory symptoms of nasal mucosa in AR rats, which may be mediated by the inhibition of NLRP3 inflammasome activation and NOD2-mediated NF-κB activation. These findings indicate that APS has the potential to be used as a therapeutic agent for AR.

Background It is known that miR-532-5p is critical for neuronal differentiation. However, the role of miR-532-5p in depression remains unknown. This study aimed to investigate the role and mechanism of miR-532-5p in major depressive disorder (MDD). Methods The depression mice model was established by chronic unpredictable mild stress (CUMS) and confirmed by forced swimming test (FST) and sucrose preference test (SPT). The role of miR-532-5p in MDD was detected by tail suspension test (TST), FST, SPT and SIT. QRT-PCR was used to detect the expression of miR-139-5p in hippocampus and BV-2 microglia of mice. ELISA and Western blotting were used to detect the expression of the nitric oxide synthase (NOS), proinflammatory cytokines (IL-6, IL-1β, TNF-α, and MCP-1) and transcriptional activator 3 (STAT3). Luciferase reporter assay was used to verify the downstream target genes of miR-532-5p. Results MiR-532-5p was significantly reduced in the hippocampus of mice treated with CUMS. Overexpression of miR-532-5p significantly reduced CUMS-induced depression-like behaviors and suppressed the expression of IL-6, IL-1β, TNF-α and MCP-1. MiR-532-5p directly targeted signal transducers and STAT3 in BV2 cells. In addition, overexpression of miR-532-5p restrained the raise of inducible NOS and IL-6, IL-1 β, TNF-α and MCP-1 in LPS-exposed BV2 cells. Conclusion This study indicates that miR-532-5p plays an important role in CUMS-induced depression-like behaviors by targeting STAT3, and miR-532-5p may be a potential target for MDD therapy.

The emergence and re-emergence of Zika virus (ZIKV), is a cause for international concern. These highly pathogenic arboviruses represent a serious health burden in tropical and subtropical areas worldwide. Despite these burdens, antiviral therapies do not exist, and inhibitors of ZIKV are therefore urgently needed. To elucidate the anti-ZIKV effect of lycorine, we used reverse transcription-quantitative real-time PCR (qRT-PCR), immunofluorescence, Westernwestern blot, and plaque forming assay to analyse viral RNA (vRNA), viral protein, progeny virus counts, and validated inhibitors in vitro using a variety of cell lines. Additionally, we found that lycorine acts post-infection according to time-of-addition assay, and inhibits RdRp activity. Lycorine protected AG6 mice against ZIKV-induced lethality by decreasing the viral load in the blood. Due to its potency and ability to target ZIKV infection in vivo and in vitro, lycorine might offer promising therapeutic possibilities for combatting ZIKV infections in the future., Highlights • Lycorine has good antiviral activity in vitro and in vivo. • Lycorine inhibits RNA replication and protein synthesis. • Lycorine inhibits RdRp activity. • Lycorine increases mouse survival and reduces viremia.

Diarrhea-predominant irritable bowel syndrome (IBS-D) is a common chronic functional gastrointestinal disorder. MicroRNAs (miRNAs) have been identified to be involved in different physiological and pathological processes. In this study, the role of miRNA-29a in the potential mechanism underlying the function of the intestinal mucosal barrier in IBS-D was analyzed. Human intestinal mucosal epithelia from patients with IBS-D (diagnosed as meeting the Rome IV criteria) and healthy volunteers were collected. An IBS-D mouse model was established via induction with trinitro-benzene-sulfonic acid (TNBS), and the mice were injected with miRNA-29a inhibitor. Using transmission electron microscopy (TEM), the epithelial ultrastructure of the human intestinal mucosa was examined. Using reverse transcription-quantitative polymerase chain reaction (RT-qPCR) analysis, the expression level of miRNA-29a was assessed. ELISA was used to analyze the activity of D-lactate (D-LA) and diamine oxidase (DAO). Through immunohistochemistry, RT-qPCR and western blotting, the expression of tight junction protein ZO-1 (ZO-1) and claudin-1 (CLDN1) was examined. In the human intestinal mucosal epithelia from patients with IBS-D, miRNA-29a was upregulated, ZO-1 and CLDN1 were downregulated, and the junctional complex (JC) was faint and discontinuous. In the IBS-D mouse model, treatment with miRNA-29a inhibitor downregulated D-LA and DAO activity, and increased the expression of ZO-1 and CLDN1 in the intestinal mucosal epithelium. In conclusion, the present study revealed that miRNA-29a is involved in the pathogenesis of IBS-D, probably by downregulating ZO-1 and CLDN1 expression, suggesting that miRNA-29a is likely to be an important regulator of intestinal barrier function and could be a possible therapeutic target for IBS-D.

Based on the research achievements of the Haiyuan 1 oscillating buoy wave power generation device, this paper proposes the innovation about a group array of the buoy to increase the output of power generation and realize large-scale power generation. Firstly, the conversion efficiency of wave energy captured by buoys of arrays 3, 6, and 9 for power generation is theoretically estimated. According to wave theory, the energy conversion efficiency of buoy increases with the increase of array number. Then, the modeling and simulation of the buoy array wave energy generation system are carried out by using AMESim simulation software. Through the simulation and optimization of the parameters of the components in the array buoy storage power generation system, the stability and efficiency of the wave power generation device under different array buoys are studied. The simulation results show that by adjusting the volume of the accumulator, precharge pressure and the displacement of the hydraulic motor, the power generation system can not only realize the continuous power output, but also the power output increases with the increase of the number of accumulators. It is proved that the feasibility and rationality of the designed buoy power generation system in group array module, and the research results can make a reliable technical reference for the construction and implementation of large-scale wave power generation system.

The oscillating buoy wave energy converter (OBWEC) is a popular and widely used wave energy converter (WEC) due to the simplicity of its structure and its strong adaptability. In this paper, the “Haiyuan No. 1″ OBWEC is taken as the research object, in which the buoys adopt heave mode of motion. In order to further enhance the hydrodynamic performance of the buoys, an improved scheme with longitudinal swing motion is proposed through theoretical analysis for the buoy. A 2D numerical wave tank (NWT) model based on the RANS equation is established by using CFD software. The numerical model is applied to compare and analyze the hydrodynamic performance differences of the buoy under two different motion forms: heaving and longitudinal swing, and the consistency of the numerical results is validated by the experimental data under regular waves. Finally, the irregular wave data obtained during the sea trial of the “Haiyuan No. 1″ OBWEC is used to numerically simulate the two buoys. The results show that the buoy with longitudinal swing motion is more effective in improving the hydrodynamic performance compared to the buoy with heave motion, in both regular and irregular waves. The conclusions of this paper can serve as a guide to improve the design of the hydrodynamic performance of the buoy.

With the gradual decrease of land resources, people pay more and more attention to the development of marine resources, and the use of marine wave energy has become the focus of people. In this paper, the pipeline parameters and pipeline model of the floating power generation device are set up. The comprehensive simulation of the hydraulic system is established by AMESim software. Under the condition of ensuring the normal results of the simulation model, the simulation model is solved by the solver to obtain the pressure fluctuation in the pipeline. The influence of inner diameter and length of pipeline on pressure fluctuation is analyzed. At the same time, through the feedback of pipeline pressure and the actual situation analysis, the feasible suggestions are given, which has certain reference significance for the design of hydraulic pipeline and provides theoretical support for the actual large wave power generation.

Irritable bowel syndrome (IBS) is a functional bowel disease with a complicated etiopathogenesis, often characterized by gastrointestinal motility disorder and high visceral sensitivity. IBS is a comprehensive multi-systemic disorder, with the interaction of multiple factors, such as mental stress, intestinal function and flora, heredity, resulting in the disease. The existence of a common mechanism underlying the aforementioned factors is currently unknown. The lack of therapies that comprehensively address the disease symptoms, including abdominal pain and diarrhea, is a limitation of current IBS management. The current review has explored the role of the SCF/c-Kit receptor/ligand system in IBS. The SCF/c-Kit system constitutes a classical ligand/receptor tyrosine kinase signaling system that mediates inflammation and smooth muscle contraction. Additionally, it provides trophic support to neural crest-derived cell types, including the enteric nervous system and mast cells. The regulation of SCF/c-Kit on the interstitial cells of Cajal (ICC) suggest that it may play a key role in the aberrant intestinal dynamics and high visceral sensitivity observed in IBS. The role of the SCF/c-Kit system in intestinal motility, inflammation and nerve growth has been reported. From the available biomedical evidence on the pathogenesis of IBS, it has been concluded that the SCF-c-Kit system is a potential therapeutic target for rational drug design in the treatment of IBS.

Some patients with irritable bowel syndrome (IBS) have visceral hypersensitivity, which contributes to their abdominal pain. miRNA-29 was detected to be significantly upregulated in colonic tissues of patients with IBS. However, it is unknown whether miRNA-29a is involved in the visceral hypersensitivity pathogenesis of IBS. This study aimed to investigate whether miRNA-29a participates in visceral hypersensitivity in IBS. We investigated miRNA-29a in intestinal biopsies collected during endoscopy of patients with IBS (n = 10) and healthy volunteers (control) (n = 10). In addition, a water avoidance stress (WAS)-induced visceral hypersensitivity IBS mouse model was established. The abdominal withdrawal reflex (AWR) scores of mice in response to colorectal distention were used to assess visceral sensitivity. Reverse transcription quantitative-polymerase chain reaction (RT-qPCR) was used to measure miRNA-29a levels. Immunofluorescence, RT-qPCR and western blot were used to measure 5-HT7 receptor (HTR7) levels. Bioinformatic analysis and luciferase reporter assays were used to detect the direct relationship between miRNA-29a and HTR7. Finally, alterations in the levels of HTR7 and miRNA-29a were measured in the human intestinal epithelial cell line NCM460 after transfection with miRNA-29a inhibitor or mimic. Intestinal tissues from patients with IBS and WAS-induced IBS mice had increased levels of miRNA-29a, but reduced levels of HTR7. MiRNA-29a knockout resulted in overexpression of HTR7 and attenuated visceral hyperalgesia in WAS-induced IBS mice. HTR7 was a direct target of miRNA-29a. Based on analyses of intestinal tissue samples from patients with IBS and WAS-induced miRNA-29a–/– mice, miRNA-29a plays a role in the visceral hyperalgesia pathogenesis of IBS, probably through regulating HTR7 expression.

Ulcerative colitis (UC) is a widely studied inflammatory disease associated with differential expression of genes involved in immune function, wound healing, and tissue remodeling. MicroRNAs have been reported to play a role in various cancer types. However, the mechanism of how microRNAs regulate UC remains unclear.In the present study, we investigated the role of miR-19a and tumor necrosis factor (TNF)-α in human colon tissues with UC and dextran sodium sulfate (DSS)-induced experimental colitis.We identified that the expression of miR-19a was significantly reduced and TNF-α was remarkably increased in human colon tissue with UC. Moreover, this observation of miR-19a and TNF-α was also occurred in DSS-treated mice colitis. Further, we observed that miR-19a directly regulated TNF-α expression because miR-19a can suppress the expression of wild-type TNF-α reporter, but not the mutant form. The expression of inflammatory factors TNF-α, IL-8, and GM-GSF were significantly elevated upon application of miR-19a inhibitor.Taken together, this study determines the levels of miR-19a and TNF-α in both DSS-induced experimental murine colitis and human UC and further demonstrates that miR-19a might directly regulate TNF-α. The findings may provide a new insight in the clinical treatment of UC.

Background:To systematically evaluate the clinical efficacy and safety of Kangfuxin liquid (KFXL) combined with aminosalicylic acid (ASA) in treating ulcerative colitis (UC).Methods:The PubMed, Cochrane Library, Embase, CBM, Wan fang, the Chinese Scientific Journal Database (VIP), and Chines

We have manufactured a novel human foldable capsular vitreous body (FCVB). In this article, we determine whether the human FCVB releases levofloxacin in vitro and evaluate the release characteristics.Levofloxacin at dosages of 100, 250, and 500 μg/mL in 4.0 mL H2O was injected into the novel human FCVB; then, the capsules were immersed in cups of modified Franz diffusion cells. Two hundred microliters of liquid was aspirated at intervals of 10, 20, 40, 60, 120, 180, 240, 300, and 360 min. The levofloxacin concentrations in the cups were detected using the liquid chromatographic-tandem mass spectrometry (HPLC-MS/MS) method. Five hundred μg/mL levofloxacin liquor at volumes of 4.0, 5.0, and 6.0 mL was injected into the novel human FCVB, and the release characteristics were detected for each. The capsules of the FCVB were observed under scanning electron microscopy before and after the release study.The novel human FCVB released levofloxacin in a time-dependent manner under 100, 250, and 500 μg/mL dosages from 10 to 360 min. Particularly in the 250 μg/mL levofloxacin group, the concentration (Q) had a good linear relationship with time (t(1/2)), Q=1.3381t(1/2)+10.2818 (r=0.9954, Higuchi equation). The human FCVB released levofloxacin in a dose-dependent manner under 100, 250, and 500 μg/mL dosages from 10 to 360 min in the total tendency, but the 500 μg/mL group released less levofloxacin than the 250 group at 300 and 360 min. The human FCVB did not release levofloxacin in a volume-dependent manner with 4.0, 5.0, and 6.0 mL volume. The 4.0-mL volume group released more levofloxacin than the other 2 volume groups. Numerous 300-nm-mili apertures were observed in the capsule of the FCVB at the beginning and end of this observation.A human FCVB can sustainably, in vitro, mechanically release levofloxacin in both a time-dependent and dose-dependent manner, but not in a volume-dependent manner. This study sets forth a novel combined research and therapeutic strategy as a vitreous substitute and intravitreal drug delivery system for the treatment of bacterial endophthalmitis.

The applicable offshore platform cooling system is very important to the diesel power station, it not only protects the pipe and valve against the seawater erosion, but also improves the recycling energy to the cooling system. According to the features of the jack-up platform, the text analyses 4 sorts of cooling conditions on the ocean oil-281 platform and presents the virtues of the cooling system.

The flow field in the pipe system of pile extractor of a jack-up oil platform is simulated by solving Reynolds-averaged Navier-Stokes (RANS) equations, which is closed by two equation turbulence model. Several different kinds of fluid were considered in the numerical simulation. Pressure distribution on the inner surface of the pipes and the jetting flow rate were calculated, and some flow details were shown. The design of the pile extractor of jack-up platform can be optimized by analyzing the characteristics of the flow in the pipe system.

According to the geologic features where the Sheng-li No.5 workover platform locates, the text states a jetting valve of leg shoe. The jetting valve could prevent from the jam of mud and sand when the platform leg shoe penetrates the seabed, so it is particularly applied to the muddy sea waters such as Bohai.