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Many chronic inflammatory diseases are attributed to disturbances in host-microbe interactions, which drive immune-mediated tissue damage. Depending on the anatomic setting, a chronic inflammatory disease can exert unique local and systemic influences, which provide an exceptional opportunity for understanding disease mechanism and testing therapeutic interventions. The oral cavity is an easily accessible environment that allows for protective interventions aiming at modulating the immune response to control disease processes driven by a breakdown of host-microbe homeostasis. Periodontal disease (PD) is a prevalent condition in which quantitative and qualitative changes of the oral microbiota (dysbiosis) trigger nonresolving chronic inflammation, progressive bone loss, and ultimately tooth loss. Here, we demonstrate the therapeutic benefit of local sustained delivery of the myeloid-recruiting chemokine (C-C motif) ligand 2 (CCL2) in murine ligature-induced PD using clinically relevant models as a preventive, interventional, or reparative therapy. Local delivery of CCL2 into the periodontium inhibited bone loss and accelerated bone gain that could be ascribed to reduced osteoclasts numbers. CCL2 treatment up-regulated M2-macrophage and downregulated proinflammatory and pro-osteoclastic markers. Furthermore, single-cell ribonucleic acid (RNA) sequencing indicated that CCL2 therapy reversed disease-associated transcriptomic profiles of murine gingival macrophages via inhibiting the triggering receptor expressed on myeloid cells-1 (TREM-1) signaling in classically activated macrophages and inducing protein kinase A (PKA) signaling in infiltrating macrophages. Finally, 16S ribosomal ribonucleic acid (rRNA) sequencing showed mitigation of microbial dysbiosis in the periodontium that correlated with a reduction in microbial load in CCL2-treated mice. This study reveals a novel protective effect of CCL2 local delivery in PD as a model for chronic inflammatory diseases caused by a disturbance in host-microbe homeostasis. [ABSTRACT FROM AUTHOR]

Teacher educators' distinct and dual task of educating future teachers includes using digital tools to support students' ongoing learning while exemplifying appropriate teaching strategies where the use of digital tools, such as response systems (RSs), are commonly occurring. RSs have been used in higher education for a long time, and many studies discuss how larger student groups answering multiple-choice questions during lectures contribute to student participation and learning. However, there is limited research on RSs, particularly related to teacher education. Therefore, this interview study aims to explore for what purposes teacher educators use RSs in teaching and what advantages and limitations they experience. In the thematic analysis, we found that the teacher educators used RSs to teach simultaneously as they were role models on how to use digital tools for learning. They used anonymous open-text answers more than multiple-choice questions to support student participation, immediately assess, and provide feedback in both larger and smaller groups. The complexity of time management connected to the use of RSs was highlighted. RSs were also used to initiate discussions with the teacher students about the purposes, advantages, and limitations of using digital tools for learning. [ABSTRACT FROM AUTHOR]

In 2012, a private University in South Texas sought to prepare eight cohorts of 25 nursing, optometry, pharmacy, physical therapy, and health care administration students with an interprofessional education activity as a model for collaborative learning. The two semester interprofessional activity used a blended model (Blackboard Learn®, face-to-face and simulated experiences) to teach the core competencies of Interprofessional Education and Collaborative Practice (IPECP). The organizing framework for the curriculum sequence included concepts of health promotion, prevention and intervention embedded in the Web of Causation (Primary, Secondary, Tertiary care). One hundred ninety-eight students participated in the interprofessional educational activity. Several of the disciplines used multiple methods to invite students to participate in this voluntary effort. One discipline provided a means by which students could select this activity as an elective and another integrated the activity into an existing course. While not completely new, transforming the way pre-licensed students and faculty in health professions education experience clinical and didactic environments is an imperative. Interprofessional education and clinical practice must be an intentional preparation that needs and requires support from university administration for successful implementation and sustainability.

Scalesia pendunculata Hook.f. is the dominant tree in several highlands' areas of the Galapagos Archipelago, yet in inhabited islands the conversion to agricultural fields has reduced its cover. The transition to agroforestry systems including the species shows promising scenarios to restore its cover and to provide ecosystem services such as carbon sequestration. Here, based on field gathered data, we model the potential contribution of S. pedunculata stands in the carbon sequestration of Galapagos. Between 2013–2021, 426 S. pedunculata seedlings were planted in the highlands of Santa Cruz and Floreana islands using several restoration technologies, and their height and survival were monitored every three months. A sub-sample of 276 trees alive since 2020 was used to estimate the DBH based on plant age and height. Based on scientific literature, biomass and carbon content were estimated across time. The final modelling included the density of plants in the restoration sites, estimated DBH, potential survival by restoration treatment, and a Brownian noise to add stochastic events. Overall, survival of S. pedunculata was high in control and slightly increased by most restoration treatments. A stand of 530 trees/ha was projected to sequester ~21 Mg C/ha in 10 years. If this is replicated over all Galapagos coffee production would contribute to the reduction of -1.062% of the Galapagos carbon footprint for the same period. This study adds to compiling benefits of restoring Galapagos flora. [ABSTRACT FROM AUTHOR]

During 2016-2017, Puerto Rico had active transmission of Zika virus (ZIKV). During January 27, 2016-June 10, 2017, the Puerto Rico Department of Health (PRDH) reported 40,357 confirmed cases of ZIKV [...]

Zika virus infection during pregnancy can cause brain abnormalities, microcephaly, and other birth defects in exposed offspring (1,2). Although transmission of Zika virus primarily occurs through the bite of an [...]