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Multiplexed gratings can provide multiple independent resonance modes for control of light–matter interactions. Here, we propose a method to digitally multiplexing rectangular metal gratings that can be fabricated with planar processing technologies, and adaptable to various structure and material systems. As different logic operators (OR, AND, XOR) may be applied in the multiplexation, we investigate their influences on plasmonic resonance properties of as–multiplexed metallic gratings, as well as dependences of their performances on structure features of the constitutive gratings. It’s concluded that, generally, the OR–operation on constitutive rectangular metal gratings with a medium ratio of ridge–width to period generates optimal multiplexed gratings that have excellent plasmonic resonance figures and are less critical for fabrication. The proposed structures may be applied in developing sensors and optoelectronic devices for improved performances or novel functions.

DNA profiling of short tandem repeats (STRs) is the primary method for genotyping forensic samples. However, degraded DNA and trace samples are still major problems for commercial 5- or 6-dye STR kits. In order to improve the performance of this method, we developed a novel 8-dye STR multiplex system containing 18 autosomal loci (D3S1358, D1S1656, TPOX, D16S539, vWA, D6S1043, D2S1338, CSF1PO, D19S433, D7S820, FGA, D8S1179, D5S818, D13S317, TH01, D21S11, D12S391, and PentaD) and the sex-determining locus Amelogenin, with all fragments smaller than 330 bases. Validation was carried out as recommended by the Scientific Working Group on DNA Analysis Methods. The results showed that complete profiles were obtainable when the input DNA was as low as 0.0625 ng. Full profiles were obtained even in the presence of inhibitors such as humic acid (

A system has been developed to identify 11 genetic markers associated with the risk of obesity: rs10852521, rs11075990, rs1121980, rs1421085, rs1477196, rs17817449, rs3751812, rs7206790, rs8047395, rs9940128 (FTO gene) and rs1137101 (LEPR gene) by minisequencing (SNaPshot analysis). The conditions for carrying out the amplification and minisequencing reactions, as well as the compositions of the reaction mixtures, were optimized so that the analysis was carried out for all 11 markers simultaneously. The resulting system was tested and showed a high degree of reproducibility and sensitivity required for the detection of these polymorphisms.

Viruses are common causes of food- and waterborne diseases worldwide. Conventional identification of these agents is based on cultivation, antigen detection, electron microscopy, or real-time PCR. Because recent technological advancements in detection methods are focused on fast and robust analysis, a rapid multiplexing technology, which can detect a broad spectrum of pathogenic viruses connected to food or water contamination, was utilized. A new semiquantitative magnetic bead–based multiplex system has been designed for simultaneous detection of several targets in one reaction. The system includes adenoviruses 40/41 (AdV), rotavirus A (RVA), norovirus (NoV), hepatitis E virus (HEV), hepatitis A virus (HAV), and a target for external control of the system. To evaluate the detection system, interlaboratory ring tests were performed in four independent laboratories. Analytical specificity of the tool was tested on a cohort of pathogenic agents and biological samples with quantitative PCR as a reference method. Limit of detection (analytical sensitivity) of 5 × 100 (AdV, HEV, and RVA) and 5 × 101 (HAV and NoV) genome equivalents per reaction was reached. This robust, senstivie, and rapid multiplexing technology may be used to routinely monitor and manage viruses in food and water to prevent food and waterborne diseases.

In the present study, a novel multiplex system, AGCU X-InDel 38 kit, was designed to amplify 38 X-InDel markers and amelogenin in a single Polymerase Chain Reaction (PCR). To demonstrate the suitability and efficiency for forensic applications, a series of validation experiments were conducted, including sensitivity, species specificity, reproducibility, stability, case samples, balance of peak height, size precision, as well as allele frequency and forensic parameter analysis. The results showed that AGCU X-InDel 38 kit was capable to get full profiles even with 62.5 pg of template DNA, and full profiles can be obtained when hematin concentration ≤25 μmol/L, or hemoglobin concentration ≤50 μmol/L, showing good tolerance to six common inhibitors. Moreover, the analyzed case samples indicated that AGCU X-InDel 38 kit had better performance for degraded and trace DNA samples. The 200 unrelated males from Guangdong Han population showed that the combined PDMale and PDFemale were both more than 0.999999999, and the combined MECKrüger, MECKishida, and MECDesmarais Duo were 0.999369481, 0.999999917, and 0.999941556, respectively. Robust discrimination capability of this novel multiplex system could be demonstrated through the high values of forensic parameters. In conclusion, AGCU X-InDel 38 kit is sensitive, precise, reproducible, and highly informative and could be used as a complementary tool for complex and challenging kinship cases.

In this paper, we present a carrier interferometry-aided chaotic orthogonal frequency division multiplex system for secure information transmissions over wireless channels, with an aim to address the performance degradation issues induced by the imperfect operating conditions of oscillators and amplifies. Therein the data are encoded by chaotic chips, then are spread by the carrier interferometry (CI) codes over multiple sub-carriers. Thus, the signals to be transmitted via multiple sub-carriers can behave in a chaotic fashion to achieve secure transmissions over broadcasting wireless channels, whereas the spreading in the frequency domain achieved by CI codes is exploited to reject the phase noise and suppresses the peak to average power ratio (PAPR). Furthermore, we derive the signal-to-noise-plus-interference ratio expressions for bit error rate (BER) analysis. Simulations are performed over additive white Gaussian channel and Rayleigh fading channels. The results verify the effectiveness of theoretical analysis and demonstrate that our presented system can achieve better PAPR and BER performances than those of the benchmark schemes.

X-chromosome short tandem repeat (X-STR) markers are a powerful complementary system used for paternity and forensic casework. This study presents the development and validation of a new highly efficient multiplex-fluorescent-labeled 19 X-STR typing system, including DXS10079, DXS101, DXS10135, DXS10162, DXS6795, DXS6800, DXS6803, DXS6807, DXS6809, DXS6810, DXS7133, DXS7423, DXS981, DXS9902, DXS9907, GATA165B12, GATA172D05, GATA31E08 and HPRTB along with sex-typing locus, amelogenin. The system was validated according to guidelines issued by the Scientific Working Group on DNA Analysis Methods. Allele frequency and forensic parameters were investigated from 1085 (494 males and 591 females) unrelated Beijing Han individuals, the combined power of discrimination by the 19 X-STR loci in females and males, as well as the combined mean exclusion chance in trios and duos, were 0.999999999999999995, 0.99999999995, 0.9999999995, and 0.9999996, respectively. The results demonstrate that this multiplex system is robust and reliable, and considered to be a powerful tool for forensic application.

Performance of PowerPlex Fusion 6C kit (PP F6C) was assessed in 374 unrelated individuals belonging to Madhya Pradesh, an Indian state. The study evaluated the forensic parameters for the loci included in PP F6C Multiplex System. The combined discrimination power (CPD) and combined exclusion power (CPE) were 1 and 0.999999995, respectively, for all 23 autosomal STR loci. SE33 showed the greatest power of discrimination (0.990) in the studied population, whereas TPOX showed the lowest (0.843). The availability of three Y-STR loci in the Multiplex System is suitable for assessing male contribution and amelogenin deletion in a single Multiplex PCR simultaneously. The study also presents the first global report on polymorphism in the Indian population on SE 33 autosomal STR loci and PP Fusion 6C Multiplex System. The results revealed that the studied STR Multiplex System is highly polymorphic and suitable for forensic purposes.

The multiplex data-acquisition system for the accelerator LUE-200 control is presented. The data acquisition of the group of tasks (system channels) is synchronized with the acceleration cycle. The operations of the group of channels and the system node are allocated considering the development of the minimal system structure. The number of the channels serviced during the cycle and the data flow are defined for applicable synchronization modes and possible queue for servicing tasks. The main characteristics of the channels and the system node are optimized to reduce the waiting time for the supervision of the accelerator short cycle, fast protection, and feedback control.

Scientific reports 9(1), 15479 (2019). doi:10.1038/s41598-019-52077-w, Published by Macmillan Publishers Limited, part of Springer Nature, [London]

A Y-STR multiplex system has been developed with the purpose of complementing the widely used 17 Y-STR haplotyping (AmpFlSTR Y Filer® PCR Amplification kit) routinely employed in forensic and population genetic studies. This new multiplex system includes six additional STR loci (DYS576, DYS481, DYS549, DYS533, DYS570, and DYS643) to reach the 23 Y-STR of the PowerPlex® Y23 System. In addition, this kit includes the DYS456 and DYS385 loci for traceability purposes. Male samples from 625 individuals from ten worldwide populations were genotyped, including three sample sets from populations previously published with the 17 Y-STR system to expand their current data. Validation studies demonstrated good performance of the panel set in terms of concordance, sensitivity, and stability in the presence of inhibitors and artificially degraded DNA. The results obtained for haplotype diversity and discrimination capacity with this multiplex system were considerably high, providing further evidences of the suitability of this novel Y-STR system for forensic purposes. Thus, the use of this multiplex for samples previously genotyped with 17 Y-STRs will be an efficient and low-cost alternative to complete the set of 23 Y-STRs and improve allele databases for population and forensic purposes.

The 19 X-STRs multiplex system is a PCR-based amplification kit that facilitates simultaneous amplification of 19 X-chromosomal STR loci (i.e. DXS7423, DXS10148, DXS10159, DXS6809, DXS7424, DXS8378, DXS10164, DXS10162, DXS7132, DXS10079, DXS6789, DXS101, DXS10103,DXS10101, HPTRB, DXS10075, DXS10074, DXS10135, and DXS10134). Eleven loci were extensively used in an Investigator Qiagen Argus X-12 (DXS7423, DXS10148, DXS8378, DXS10162, DXS7132, DXS10079, DXS10103, DXS10101, HPTRB, DXS10074, and DXS10135). In this research, the multiplex system was tested for detection sensitivity, DNA mixtures, inhibitor tolerance and species specificity; SWGDAM Validation Guidelines - Approved December 2012 were followed for the human fluorescent STR multiplex PCR reagent. Samples from 181 unrelated Zhejiang Han individuals (121 males and 60 females) were typed using this multiplex system. The results show that this 19X-STRs multiplex system is a robust and reliable amplification means to facilitate forensic and human identification testing.

The 18 loci multiplex system has been instigated for co-amplification and fluorescent detection of Amelogenin and 17 STRs, including 10 MiniSTRs (CSF1PO, D18S51, D7S820, D2S1338, TPOX, D13S317, FGA, D5S818, D21S11, D16S539), SE33, Penta E, Penta D, and four Y-STRs (DYS385a/b, DYS438, DYS392). This multiplex system was developed for the simultaneous analysis of compromised DNA samples, Y-amelogenin marker mutation, motherless paternity issues where single allele sharing occurs at autosomal STRs in unrelated individuals, and other complex forensic cases. Selection of loci, primers, and allelic ladders were designed and created in-house with a design strategy to work in this multiplex. The multiplex system was evaluated by sensitivity, specificity, stability, precision and accuracy, case-type samples, mixture studies, PCR-based and population distribution studies to establish the robustness and reliability of the system as the current requirements of the forensic case work. Among all the markers evaluated for this study, 209 alleles including 44 variants were observed with combined power of discrimination, combined power of exclusion, and the combined probability of matching calculated as 0.999999999999999999893916339344, 0.999993816173890, and 5.90019 × 10−19, respectively. Due to highly polymorphic characteristics of these loci particularly SE33 and Penta E which are most discriminatory (PD = 0.991 and 0.983, respectively) in the Pakistani population, this multiplex would be highly valuable for individual identification in complex forensic cases and paternity issues as well as population database.

The present investigation assessed the allele frequencies and forensic parameters for the loci included in PowerPlex 21 multiplex system (Promega, USA) in 101 unrelated individuals residing in the state of Gujarat, India. The study likewise introduces the first global report on polymorphism on Penta D and Penta E autosomal STR loci from the population of Gujarat, India. The obtained outcomes in the study revealed that the considered STR multiplex system is highly polymorphic and suitable for forensic genetic analysis.

Wavelength division multiplex (WDM) system with on / off keying (OOK) modulation and direct detection (DD) is generally simple to implement, less expensive and energy efficient. The determination of the possible design capacity limit, in terms of the bit rate-distance product in WDM-OOK-DD systems is therefore crucial, considering transmitter / receiver simplicity, as well as energy and cost efficiency. A 32-channel wavelength division multiplex system is designed and simulated over 1000 km fiber length using Optsim commercial simulation software. The standard channel spacing of 0.4 nm was used in the C-band range from 1.5436-1.556 nm. Each channel used the simple non return to zero - on / off keying (NRZ-OOK) modulation format to modulate a continuous wave (CW) laser source at 40 Gbps using an external modulator, while the receiver uses a DD scheme. It is proposed that the design will be suitable for long haul mobile backbone in a national network, since up to 1.28 Tbps data rates can be transmitted over 1000 km. A bit rate-length product of 1.28 Pbps.km was obtained as the optimum capacity limit in 32 channel dispersion managed WDM-OOK-DD system., Comment: Accepted for publication in Journal of Optical Communications - De Gruyter

The 24-locus multiplex system allows co-amplification and fluorescent detection of 24 loci (23 STR loci and Amelogenin), including STR loci in the Combined DNA Index System (CODIS) and the ESS (European Standard Set) as well as five additional loci (D2S1338, D6S1043, D19S433, Penta D and Penta E) commonly used in commercial kits. It facilitates data sharing and minimizes adventitious matches within national or between international DNA databases. Additionally, the system can amplify directly from blood and buccal samples spotted on filter paper and swabs and reduce the cycling time to less than one hour and a half. Primers, internal size standard, allelic ladders and matrix standard set were designed and created in-house with a design strategy to work in this multiplex. Developmental validation experiments followed the Scientific Working Group on DNA Analysis Methods (SWGDAM) and the Chinese National Standard (GA/T815-2009) guidelines. The system was evaluated by species specificity, sensitivity, stability, precision and accuracy, case-type samples, population, mixture and PCR-based studies. The results demonstrate that the 24-locus multiplex system is a robust and reliable identification assay as required for forensic DNA typing and databasing.

In this work we have developed a multiplex microarray system capable of detecting VEGF165 and thrombin. We recently described a Sandwich Aptamer Microarray (SAM) for thrombin detection feasible for use in multiplex microarrays; here we describe a new aptasensor for VEGF165 detection employing Vap7 and VEa5, two DNA aptamers recognizing different sites of the protein. The aptamers were modified to be adapted to the solid phase platform of SAM and their capability to simultaneously recognize VEGF165 by forming a ternary complex was analyzed in solution. Having so defined the best tandem arrangement of modified aptamers, we set up the aptasensor for VEGF165, and finally analyzed the multiplex system with the two aptasensors for the simultaneous detection of VEGF165 and thrombin. The results indicate that each sandwich is specific, even when the two proteins are mixed. The system performance is consistent with the behavior evidenced by the biochemical analysis, which proves to be valuable to drive the evaluation and refinement of aptamers prior to or along the development of a detection platform. Since thrombin upregulates VEGF expression, the simultaneous recognition of these two proteins could be useful in the analysis of biomarkers in pathologies characterized by neo-angiogenesis.

This study aimed to develop a short tandem repeat (STR) multiplex system, made up of 22 highly informative STR loci, for application in forensic genetics. The system comprised 21 polymorphic autosomal loci (D3S1358, TH01, D21S11, D18S51, Penta E, D5S818, D13S317, D7S820, D16S539, CSF1PO, Penta D, vWA, D8S1179, TPOX, FGA, D2S441, D17S1301, D19S433, D18S853, D20S482, and D14S1434) and the amelogenin gene locus. Strategies were developed to overcome the challenges involved in creating a multiplex system. Based on the literature and available databases, the STR loci were selected with the aim to obtain discriminatory markers, and followed specific criteria for this purpose. Primers were projected using the Primer3 software, and AutoDimer was used to evaluate possible interactions between them. The 22 selected STR loci were validated individually and jointly, both to assess their sensitivity and to test the efficiency of the multiplex system. Statistical analyses were based on the genetic data of 450 unrelated individuals living in the State of Goias, thus allowing the establishment of the parameters necessary to use this system. A total of 239 alleles were detected for the 22 loci in the set, allowing for a probability of identity of 4.23 x 10-25 to be obtained. The combined power of discrimination was 0.999999999999999999999999 and the combined power of exclusion was 0.99999. Upon complete validation of the entire system, this multiplex assay was considered to be a powerful tool for application in human identification by DNA analysis.

Diffusion in a multiplex depends on the specific link distribution between the nodes in each layer, but also on the set of the intralayer and interlayer diffusion coefficients. In this work we investigate, in a quantitative way, the efficiency of multiplex diffusion as a function of the topological similarity among multiplex layers. This similarity is measured by the distance between layers, taken among the pairs of layers. Results are presented for a simple two-layer multiplex, where one of the layers is held fixed, while the other one can be rewired in a controlled way in order to increase or decrease the interlayer distance. The results indicate that, for fixed values of all intra- and interlayer diffusion coefficients, a large interlayer distance generally enhances the global multiplex diffusion, providing a topological mechanism to control the global diffusive process. For some sets of networks, we develop an algorithm to identify the most sensitive nodes in the rewirable layer, so that changes in a small set of connections produce a drastic enhancement of the global diffusion of the whole multiplex system.

We show a coding method of suspension array based on the laser induced breakdown spectroscopy (LIBS), which promotes the barcodes from analog to digital. As the foundation of digital optical barcodes, nanocrystals encoded microspheres are prepared with self-assembly encapsulation method. We confirm that digital multiplexing of LIBS-based coding method becomes feasible since the microsphere can be coded with direct read-out data of wavelengths, and the method can avoid fluorescence signal crosstalk between barcodes and analyte tags, which lead to overall advantages in accuracy and stability to current fluorescent multicolor coding method. This demonstration increases the capability of multiplexed detection and accurate filtrating, expanding more extensive applications of suspension array in life science.

This paper presents a digital dimming control circuit used in a light-emitting diode (LED) driver, allowing it to dim multiple LED strings with only two input ports which receive serial data accompanied with synchronous clock. A multiplexing technique is used to simplify the control circuit, using one DAC to transfer all the dimming messages for each string, and a real-time refresh technique is used to make the transferred dimming voltage keep a high accuracy all the time. The circuit is friendly compatible with the linear current regulator and pulsewidth modulation (PWM) dimming methods. To get a precise dimming control, we build a 10-bit segment R-2R DAC. The simulation results show the dimming voltages for each LED string keep the errors less than 0.5LSB compared with the designed voltages.

In this study, a new STRtyper-27 system, including the same Yfiler Plus loci (DYS19, DYS389I, DYS389II, DYS390, DYS391, DYS392, DYS393, DYS385a/b, DYS437, DYS438, DYS439, DYS448, DYS456, DYS458, DYS635, Y-GATA H4, DYS449, DYS460, DYS481, DYS518, DYS533, DYS570, DYS576, DYS627 and DYF387S1a/b), was established using a set of 5 fluorescent dye labels. Primers, internal size standard, allelic ladders and matrix standard set were designed and created in-house for this multiplex system. This paper describes the validation studies conducted with the STRtyper-27Y system using a 3130XL genetic analyzer for fragment length detection that included the analysis of the following parameters and aspects: sensitivity, species specificity, inhibition, haplotype concordance, precision, stutter, DNA mixtures, and stability studies with crime scene samples. The studies demonstrated, that the STRtyper-27Y system provided equivalent overall performance comparable to the latest Yfiler Plus kit, but with enhanced compatibility in terms of instrument platforms and software allowing forensic laboratories to conduct its forensic application and evaluate its performance, all in their own 5 dye Y-STR chemistry system /environment without software or instrument upgrades.

This article discusses the interplay between multiplex signal transmission in telegraphy and telephony, and sampling methods. It emphasizes the works of Herbert Raabe (1909–2004) and Claude Shannon (1916–2001) and the context in which they occurred. Attention is given to the role that the exceptional research atmosphere in Berlin during the 1920s and early 1930s played in the development of some of the ideas underlying these works, first in Germany and then in the USA, as some of the protagonists moved there. Raabe's thesis, published in 1939, describes and analyses a time-division multiplex system for telephony. In order to build his working prototype, Raabe had to develop the theoretical tools he needed and achieved a thorough understanding of sampling, including sampling with pulses of finite duration and sampling of low-pass and band-pass signals. His condition for reconstruction was known as ‘Raabe's condition’ in the German literature of the time. On the other hand, Shannon's works of 1948 and 1949 ...

This paper presents a system for the multiplex amplification of 15 loci, known as I-DNA1, which combines mini and midiSTR technology, with amplicon sizes ranging from 49 to 297 bp. I-DNA1 analyses all the STR loci included in the CODIS and the Interpol Standard Set of loci, nine of the ten European core loci and seven of the eight German core loci, making it suitable for use in identifying humans. Moreover, its high sensitivity and the small size of its amplicons mean that I-DNA1 is potentially highly useful for analysing highly degraded and/or very small DNA samples.

A multiplex system was developed to assess detection of stacked genetically modified (GM) rice (LS28 × Cry1Ac) based on multiplex polymerase chain reaction (PCR) and liquid beadarray, and the accuracy of the system was analyzed. Standard and specific bulging specific (SBS) primers with standard primers were used to simultaneously detect multiple targets in stacked events of rice. Five sets of primers for the stacked events were applied to amplify their targets, and were separated distinctly in agarose gel. A liquid beadarray assay for the stacked GM rice was performed using the multiplex PCR products, followed by target biotinylation and hybridization between biotinylated-tagged target and anti-tagged bead. Fluorescent signals of the hybridized target sequences were detected by the Luminex system. The signaling patterns were analyzed by their mean fluorescent intensity (MFI) value. Results showed that liquid beadarrays with standard and SBS primers were in complete agreement with the PCR data, and detection of the different target elements was found to be very specific with no cross reaction among samples. Therefore, our detection system developed for stacked GM crop using multiplex PCR and liquid beadarray can be a useful and efficient system for screening and analyzing multiple transgenes in a single tube for qualitative analysis.

Generally people tend to think only in terms of fuel economy and additional cost premium on vehicle price while discussing about hybrid electric vehicles (HEV). This paper tries to emphasize that the overall acceptability of a vehicle also has to do with its system level reliability. It discusses the issue of system level reliability in hybrid electric vehicles from a quantitative point of view. It also introduces a quantitative meaning to the concept of graceful degradation and mode of operation under graceful degradation condition. All these are discussed in stages, starting from a regular internal combustion engine based vehicle, and later transition of those to hybrid electric vehicles. This paper intends to drive the point that in HEV, one of the penalties for fuel economy that has to be paid comes in terms of reliability.

Y-STR genotyping is an excellent tool for the identification of male DNA masked by female DNA, particularly in sexual assault cases where, more often the male and female components cannot be separated completely. Resultant, the female component could exist prominently even in the male component after separation. When the "male DNA sample mixed with female DNA” undergoes PCR by using autosomal STR multiplex amplification process, the female DNA component is also amplified, mostly masking the male DNA. Recently launched Y-STR kit, PowerPlex Y23 (PP Y23) System is a 5-dye multiplex genotyping kit that analyzes 17 Y-STR loci, commonly available in other Y-STR multiplex kits (DYS19, DYS385a/b, DYS389I/II, DYS390, DYS391, DYS392, DYS393, DYS437, DYS438, DYS439, DYS448, DYS456, DYS458, DYS635, and Y-GATA-H4) along with that 6 new Y-STR loci (DYS481, DYS533, DYS549, DYS570, DYS576, and DYS643). The addition of 6 new markers has made the Y-STR analysis more discriminating. The purpose of the present study is to show the amplification of male DNA from a wide variety of forensic samples using half the manufacturer’s recommended reaction volume. Also the present study validates the kit for its use in Forensic casework. The results demonstrate that the PPY23 System is a robust and sensitive multiplex system, capable of giving reliable and consistent amplification results from a wide variety of forensic casework samples. Even the presence of high concentration of commonly encountered inhibitors such as hematin (from Blood) and humic acid (from soil), male/female DNA mixtures and low amounts of DNA template (15.62 pg) could not hamper the process of amplification.

Coherence multiplexing is a potentially inexpensive form of optical code-division multiple access, which is particularly suitable for short-range applications with moderate bandwidth requirements, such as access networks, LANs, or interconnects. Various topologies are known for constructing an optical transmission system in which several channels are coherence-multiplexed in one optical fiber. In this paper, the parallel array, the intrinsic reference ladder (IRL), and the discontinuous series system topologies will be further considered and compared with respect to code orthogonality requirements, theoretical performance, and some practical implementation aspects. A modification to the IRL system is proposed, resulting in a significant improvement in the theoretical performance.

This paper describes a novel spectrum sharing technique for cognitive wireless ad-hoc networks. To avoid interference caused by joint spectrum usage it is proposed to utilize the Generalized Frequency Division Multiplexing (GFDM), which is a digital multiplexing method candidate for 5G networks. Every peer-to-peer link is established using one of the subcarriers of the GFDM signal, so every node can be connected to as many its one-hop neighbors without spectrum overlapping, as many subcarriers there are configured in the GFDM signal. The GFDM waveform, formed digitally, can be modified on-line to adjust frequency usage depending on network requirements and the presence of legacy users, allowing occupation of licensed bandwidth. To detect the vacant subcarriers, the cyclostationary detector is proposed, which allows to distinguish between spectrum holes, neighbor nodes radiation and the signals of licensed users.

The paper characterizes ICT networks used in urban buses. It describes the multiplex system manufactured by Continental VOD and the ELSY multicomputer system manufactured by Thoreb, Differences in the structure and functioning of both systems were presented. Based on the Thoreb system, a test station for the modelling of a complex data bus used in city buses has been designed and built.

An original multiplex scheme is introduced, which is based on Mallat's multiresolution formulation of wavelet systems. This system is adaptable and its implementation is well matched to digital signal processors and computers. The approach termed multiresolution division multiplex (MRDM) is intensive in signal processing (SP) tools, extremely flexible and can combine a variety of tributaries at different bit rates. A broad variety of orthogonal wavelet systems can endow with MRDM and the channel waveforms, and consequently the spectral shape and system performance depend upon the selected wavelets. Demultiplex can be done efficiently, since the number of floating multiplications and additions increase only linearly with the length of signals. A Haar-based MRDM scheme is presented to illustrate the versatility of this new multiplex approach., Comment: 5 pages, 8 figures, ITS International Telecomm. Symposium, Brazil, 2006

Experimental measurements on microwave assisted Multiplex Coherent Anti-Stokes Raman Spectroscopy (M-CARS) in liquids are presented. Nanosecond electric field applied on samples allows M-CARS signal enhancement and offers potentialities to remove non resonant background.

This paper describes a multiplex architecture for mobile multimedia applications which has been considered as the underlying multiplex system in the MPEG-4 standardization effort (MPEG-4: Coding of audio-visual objects, standardization project of the ISO/IEC JTC1/SC29/WG11). Multimedia information consists of several different kinds of information which differ widely in their requirements on Quality of Service (QoS). The generic data services in today's mobile networks are not designed to comply with these QoS requirements. On the other hand, future object-based multimedia systems (e.g. MPEG-4) will use a multitude of simultaneous data streams. Many of these will have similar requirements on transmission. The proposed multiplexing architecture consists of two layers. One layer serves to bundle elementary data streams of similar QoS requirements, resulting in a small number of data streams with differing QoS requirements. The other layer serves to provide appropriate error protection for each of these streams and to form a single data stream which can be transmitted over an error prone link. Thus multiplexing overhead is minimized while error protection is adapted to the requirements of the individual elementary streams. It is argued that the lower layers dealing with protection and the error-resilient multiplex of the protected streams should be included in the network in order to avoid the problems of end-to-end error control. The system described can thus help to obtain highly efficient multimedia communication with future mobile networks.

Multi-Indel polymorphism has been considered as a useful supplementary tool for human identification. Compared with the conventional single Indel marker, it has showed obvious advantage in improving the discrimination power. Previously, we established a novel multiplex system with 20 multi-Indels witch contains 43 single InDel markers. Currently, validation studies were performed with species specificity, sensitivity, mixture study and artificially degraded samples, according to the FBI/SWGDAM guideline. The results showed (a) no fluorescent signal for forensically relevant animals was obtained, (b) complete and accurate profiles were detectable as DNA amount was 0.3ng, and (c) the reliable results of typing 20 multi-Indels could be obtained from degraded DNA. The mixture of two unrelated individuals in a ratio up to 3:7 could be recognized with all alleles. In conclusion, this study demonstrated that the multiplex system with 20 multi-Indels can be used as a useful supplementary method for human identification and paternity testing.

Summary Luminex Corp. has developed a multiplex assay system (FlowMetrix) that incorporates the most advanced digital signal processing technology into an established assay platform, enhancing its speed, economy, sensitivity, precision, and throughput. Microsphere-based assays are now multiplexed assays, capable of performing up to 64 discrete tests in a single tube from the same sample at the same time. The flow cytometer becomes an absolute measurement instrument, acquiring and analyzing the data generated by thousands of microspheres every second, and reporting the values in real time. With applications in routine immunoassay and advanced molecular diagnostics, this system should provide significant benefit to the clinical laboratory today and in the future.

We have carried out extensive validation of a multiplex system of 6 STR loci and the Amelogenin sex test to investigate the following characteristics: relative peak areas of heterozygote peaks; non-specific artefacts; stutters caused by slippage of the Taq enzyme; pull-up (an electronic artefact often associated with software). The purpose of this paper is to demonstrate how interpretation guidelines can be formulated by experimentation and analysis using casework samples. Automation allows data relating to band position (in base pairs), peak area and peak height to be easily collected and manipulated in spreadsheets or computer programs. This paves the way to designing expert systems to carry out the interpretative process.

The DVB (Digital Video Broadcasting) project was established to develop comprehensive specifications for digital television broadcasting. In three years it has completed work on a range of specifications covering broadcast delivery by all currently used means. The systems are founded on the MPEG-2 coding and multiplex specifications. Additional elements have been added to meet specific broadcasting requirements. The first DVB services in Europe, and in other parts of the world using the systems, will be of conventional quality, but the DVB systems can be used for transporting any data content, or image and sound formats, required by the programme service provider, including HDTV.

We develop a theoretical framework for the study of epidemic-like social contagion in large scale social systems. We consider the most general setting in which different communication platforms or categories form multiplex networks. Specifically, we propose a contact-based information spreading model, and show that the critical point of the multiplex system associated to the active phase is determined by the layer whose contact probability matrix has the largest eigenvalue. The framework is applied to a number of different situations, including a real multiplex system. Finally, we also show that when the system through which information is disseminating is inherently multiplex, working with the graph that results from the aggregation of the different layers is flawed., 5 pages and 3 figures. Final version to appear in PRE RC

We examined the possibility of high-density recording using shift-multiplexed holographic memory with a spherical reference beam. The use of a spherical reference beam is considered to make it possible to realize a multi-dimensional multiplex system that uses the disk track direction (x-axis), radial direction (y-axis), and disk thickness direction (z-axis); this would clearly improve the recording density when compared with the conventional angle multiplex recording. The experimental results confirm the possibility of multiple recording by 3 dimensional medium shift. Furthermore, the results indicate that a large capacity memory system of over 1 Tb/in2 can be obtained if a thick medium (about 1.5 mm) is used.

Peak-to-average power ratio (PAPR) is a major drawback in most multi-carrier communication techniques such as orthogonal frequency division multiplex system (OFDM). OFDM consists of lots of independent modulated subcarriers, as a result the amplitude of such a signal can have very large values. These large peaks increase the amount of inter-modulation distortion resulting in an increase in the error rate. In this paper we dealt with simple cyclic coding (CC) scheme having coding rates (R) of 4/7 and 11/15. The proposed coding scheme shows good PAPR reduction with any length of data sequence. The results are verified and shown for 16 point IFFT and 64 point IFFT. In this scheme add some extra parity bits to the data bits. These parity bits can correct up to one bit error.

We describe and demonstrate for the first time the use of the novel Multiple Module Multiplexer (MMMIC) [1] for a 2×2 array of new electron multiplying charge coupled device (EMCCD) based x-ray detectors [2]. It is highly desirable for x-ray imaging systems to have larger fields of view (FOV) extensible in two directions yet to still be capable of doing high resolution imaging over regions-of-interest (ROI). The MMMIC achieves these goals by acquiring and multiplexing data from an array of imaging modules thereby enabling a larger FOV, and at the same time allowing high resolution ROI imaging through selection of a subset of modules in the array. MMMIC also supports different binning modes. This paper describes how a specific two stage configuration connecting three identical MMMICs is used to acquire and multiplex data from a 2×2 array of EMCCD based detectors. The first stage contains two MMMICs wherein each MMMIC is getting data from two EMCCD detectors. The multiplexed data from these MMMICs is then forwarded to the second stage MMMIC in the similar fashion. The second stage that has only one MMMIC gives the final 12 bit multiplexed data from four modules. This data is then sent over a high speed Camera Link interface to the image processing computer. X-ray images taken through the 2×2 array of EMCCD based detectors using this two stage configuration of MMMICs are shown successfully demonstrating the concept.

1. Multiplex PCR is a valuable tool in many biological studies but it is a multifaceted procedure that has to be planned and optimised thoroughly to achieve robust and meaningful results. In particular, primer concentrations have to be adjusted to assure an even amplification of all targeted DNA fragments. Until now, total DNA extracts were used for balancing primer efficiencies; however, the applicability for comparisons between taxa or different multiple-copy genes was limited owing to the unknown number of template molecules present per total DNA. 2. Based on a multiplex system developed to track trophic interactions in high Alpine arthropods, we demonstrate a fast and easy way of generating standardised DNA templates. These were then used to balance the amplification success for the different targets and to subsequently determine the sensitivity of each primer pair in the multiplex PCR. 3. In the current multiplex assay, this approach led to an even amplification success for all seven targeted DNA fragments. Using this balanced multiplex PCR, methodological bias owing to variation in primer efficiency will be avoided when analysing field-derived samples. 4. The approach outlined here allows comparing multiplex PCR sensitivity, independent of the investigated species, genome size or the targeted genes. The application of standardised DNA templates not only makes it possible to optimise primer efficiency within a given multiplex PCR, but it also offers to adjust and/or to compare the sensitivity between different assays. Along with other factors that influence the success of multiplex reactions, and which we discuss here in relation to the presented detection system, the adoption of this approach will allow for direct comparison of multiplex PCR data between systems and studies, enhancing the utility of this assay type.

This paper presents a power-efficient system architecture for the design of multiple-channel neural recording interface. A new multiple-channel SAR ADC is proposed to facilitate multiplexing among channels, thus eliminates the need for analog multiplexer and associated buffers. The proposed ADC is verified through a 16-channel recording chip fabricated in a standard 0.13-μm CMOS technology with an active area of 1.17 mm2. The chip consumes 18 μW from a 0.5-V supply and attains a noise efficiency factor of 3.09. The average per channel power and area are 1.13 μW and 0.073 mm2, respectively, which are the lowest among existing multiple-channel designs.

The SAE J1850 protocol has been defined by SAE to a class-B protocol whose definition is given as ‘A system whereby data, e.g., parametric data, is transferred between nodes to eliminate redundant sensors and other system elements. The nodes in this form of a multiplex system typically already existed as a stand-alone module in a conventionally wired automotive vehicle’ [Wiegand 1998].