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Objective To study the clinical and prognostic significance of the preferentially expressed antigen of melanoma (PRAME) gene in the absence of specific fusion gene expression in children with B-lineage acute lymphoblastic leukemia (B-ALL). Methods A total of 167 children newly diagnosed with B-ALL were enrolled, among whom 70 were positive for the PRAME gene and 97 were negative. None of the children were positive for MLL-r, BCR/ABL, E2A/PBX1, or ETV6/RUNX1. The PRAME positive and negative groups were analyzed in terms of clinical features, prognosis, and related prognostic factors. Results Compared with the PRAME negative group, the PRAME positive group had a significantly higher proportion of children with the liver extending >6 cm below the costal margin (P<0.05). There was a significant reduction in the PRAME copy number after induction chemotherapy (P<0.05). In the minimal residual disease (MRD) positive group after induction chemotherapy, the PRAME copy number was not correlated with the MRD level (P >0.05). In the MRD negative group, there was also no correlation between them (P>0.05). The PRAME positive group had a significantly higher 4-year event-free survival rate than the PRAME negative group (87.5%±4.6% vs 73.5%±4.6%, P <0.05), while there was no significant difference between the two groups in the 4-year overall survival rate (88.0%±4.4% vs 85.3%±3.8%, P>0.05). The Cox proportional-hazards regression model analysis showed that positive PRAME expression was a protective factor for event-free survival rate in children with B-ALL (P<0.05). Conclusions Although the PRAME gene cannot be monitored as MRD, overexpression of PRAME suggests a good prognosis in B-ALL. [ABSTRACT FROM AUTHOR]

Two new dammarane-type glycosides, phlomisumbroside A (1) and phlomisumbroside B (2), together with 15 known compounds (3–17) were isolated from the leaves of Phlomis umbrosa Turcz. Their structures were established by the spectroscopic methods including 2D NMR techniques. [ABSTRACT FROM AUTHOR]

Objectives: To study the clinical and prognostic significance of the preferentially expressed antigen of melanoma ( PRAME ) gene in the absence of specific fusion gene expression in children with B-lineage acute lymphoblastic leukemia (B-ALL)., Methods: A total of 167 children newly diagnosed with B-ALL were enrolled, among whom 70 were positive for the PRAME gene and 97 were negative. None of the children were positive for MLL - r , BCR / ABL , E2A/PBX1 , or ETV6/RUNX1 . The PRAME positive and negative groups were analyzed in terms of clinical features, prognosis, and related prognostic factors., Results: Compared with the PRAME negative group, the PRAME positive group had a significantly higher proportion of children with the liver extending >6 cm below the costal margin ( P <0.05). There was a significant reduction in the PRAME copy number after induction chemotherapy ( P <0.05). In the minimal residual disease (MRD) positive group after induction chemotherapy, the PRAME copy number was not correlated with the MRD level ( P >0.05). In the MRD negative group, there was also no correlation between them ( P >0.05). The PRAME positive group had a significantly higher 4-year event-free survival rate than the PRAME negative group (87.5%±4.6% vs 73.5%±4.6%, P <0.05), while there was no significant difference between the two groups in the 4-year overall survival rate (88.0%±4.4% vs 85.3%±3.8%, P >0.05). The Cox proportional-hazards regression model analysis showed that positive PRAME expression was a protective factor for event-free survival rate in children with B-ALL ( P <0.05)., Conclusions: Although the PRAME gene cannot be monitored as MRD, overexpression of PRAME suggests a good prognosis in B-ALL.

In this study, 115 Populus nigra L. collected from Europe were used as materials. They were detected for SNPs of genes linked with lignin and holocellulose (4CL, PAL and CesA2) by TaqMan technology and correlations between SNPs and wood properties (the wood speciely gravity, fiber length, fiber width, microfibre angle, lignin, holocellulose content and a-Cellulose content) were also studied. results showed that (1) 27 SNPs were obtained in the genes, such as 4CL, PAL and CesA2 associated with lignin synthesis. Among them, 17 SNPs were transition (A-G, C-T), 10 SNPs were transversion (A-C, G-C, G-T, and A-T). (2) Three of the SNPs were discriminated, a significant negative correlation between holocellulose content of four-year old P. nigra and SNP1 was detected, and contribution ratio was 11.11%. SNP2 and SNP3 had no significant correlation with wood properties. (3) Wood properties of various genotypes of SNP1 were significantly different. CC and CT genotype relative to TT genotype had larger holocellulose content. SNP1 would be an efficient marker to choose P. nigra gene resources that have larger holocellulose content.