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225 results on '"Ding, Xueli"'

5. PROTECTIVE EFFECT OF SODIUM BUTYRATE ON INTESTINAL BARRIER IN HYPERURICEMIA MOUSE MODEL

Author

LI Yukun, LIU Xin, WANG Rong, SHEN Xi, DING Xueli, TIAN Zibin

Subjects
hyperuricemia, intestines, osmosis, butyric acid, tight junction proteins, disease models, animal, mice, Medicine
Abstract

Objective To investigate the protective effect of sodium butyrate on intestinal barrier in mice with hyperuricemia. Methods Male C57BL/6 mice were randomly divided into control group, model group, and sodium butyrate group. Mice in the model group were intraperitoneally injected with potassium oxonate (250 mg/kg) every day, and fed with high yeast diet freely. Mice in the sodium butyrate group were given 200 mg/kg sodium butyrate by gavage every day on the basis of the treatment in the model group. Mice in the control group were intraperitoneally injected and intragastrically administered with the same dose of normal saline every day. The intervention lasted for 21 d. The expression levels of ZO-1 and Occludin protein and mRNA in small intestine tissues of mice in each group were measured by Western blot, real-time quantitative polymerase chain reaction (RT-qPCR), and immunohistochemical staining. The pathological morphology of small intestine tissues in each group was observed by hematoxylin-eosin (HE) staining. The content of inflammatory factors in small intestine tissues was measured by enzyme-linked immunosorbent assay (ELISA). Results There was a significant difference in body mass among the three groups (F=4.70,P

Published
2023
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35. Wuling San Based on Network Pharmacology and in vivo Evidence Against Hyperuricemia via Improving Oxidative Stress and Inhibiting Inflammation

Author

Huang,Jing, Lin,Zhijian, Wang,Yu, Ding,Xueli, Zhang,Bing, Huang,Jing, Lin,Zhijian, Wang,Yu, Ding,Xueli, and Zhang,Bing

Abstract

Jing Huang, Zhijian Lin, Yu Wang, Xueli Ding, Bing Zhang School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing, People’s Republic of ChinaCorrespondence: Bing Zhang, School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing, People’s Republic of China, Email zhangb@bucm.edu.cnBackground: Hyperuricemia (HUA) is a major public health issue with a high prevalence worldwide. Wuling San (WLS) is an effective treatment for HUA. However, the active compounds and the related mechanism are unclear. In this study, we aimed to explore the active compounds and the underlying pharmacological mechanisms of WLS against HUA.Methods: First, a network pharmacology approach was used to detect active compounds of WLS, and potential targets and signaling pathways involved in the treatment of HUA were predicted. Then, a molecular docking strategy was used to predict the affinity between active compounds and key targets. Finally, to verify the prediction, the HUA rat model was established.Results: 49 active compounds with 108 common targets were obtained. Besides, cerevisterol, luteolin, ergosterol peroxide, beta-sitosterol, and sitosterol were identified as key active compounds. In PPI analysis, TNF, IL6, CASP3, PPARG, STAT3, and other 12 core targets were obtained. GO enrichment analysis indicated that WLS was likely to interfere with oxidative stress in the treatment of HUA, and KEGG enrichment analysis indicated multiple inflammation-related signaling pathways possibly involved in the treatment of HUA by WLS, including TNF, and NOD-like receptor, HIF-1, PI3K-Akt, and IL-17 signaling pathways. The results of molecular docking indicated that the active compounds had good binding properties to their key targets. In the validation experiments, WLS significantly reduced the levels of serum uric acid (SUA) and serum malondialdehyde (MDA). Moreover, WLS not only significantly increased the levels of total antioxidant capacity (T-AOC) an

Published
2023

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