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148 results on '"Ding, Zhaozhao"'

4. Biomimetic Vascular Grafts with Circumferentially and Axially Oriented Microporous Structures for Native Blood Vessel Regeneration.

Author

Ding, Zhaozhao, Wang, Yuanyuan, Chen, Fangfang, Hu, Xiao, Cheng, Weinan, Lu, Qiang, and Kaplan, David L.

Subjects
*VASCULAR grafts, *BLOOD vessels, *REGENERATION (Biology), *VASCULAR remodeling, *BIOMIMETIC materials, *SMOOTH muscle
Abstract

Porous grafts facilitate constructive remodeling of blood vessels. Incorporating multiple biomimetic cues to porous grafts can promote vascular regeneration. However, the fabrication of such medical devices remains challenging. Here, beta‐sheet rich silk nanofibers (BSN) are added to poly(vinyl alcohol) (PVA) solution and aggregated under a cylindric electric field to form circumferentially and axially oriented tubular structures, to simulate the endothelial and media layers of blood vessels. PVA in the aligned tubes is then crystallized through repeat freezing–thawing process to offer mechanical performances. Through tuning the ratio of BSN and PVA, the composite tubes with dual anisotropic microstructures exhibit better mechanical properties than pure PVA vascular grafts. Significantly improved cell adhesion, spreading, proliferation, and alignment are achieved. Both endothelial and smooth muscle cells show improved biological activity on the grafts due to the regulatory roles of the aligned structures. In vivo studies reveal the formation of endothelial layers within four weeks of implantation, ensuring long‐term patency. The endothelial and smooth muscle double layers are regenerated after eight months postimplantation, forming hierarchical microstructures and compositions similar to native vessels. The porous composite grafts with multiple aligned structures guide vascular remodeling to regenerate blood vessels, demonstrating potential for clinical translation. [ABSTRACT FROM AUTHOR]

Published
2024
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14. Blastocyst-Inspired Hydrogels to Maintain Undifferentiation of Mouse Embryonic Stem Cells

Author

Hang, Yingjie, primary, Ma, Xiaoliang, additional, Liu, Chunxiao, additional, Li, Siyuan, additional, Zhang, Sixuan, additional, Feng, Ruyan, additional, Shang, Qianwen, additional, Liu, Qi, additional, Ding, Zhaozhao, additional, Zhang, Xiaoyi, additional, Yu, Liyin, additional, Lu, Qiang, additional, Shao, Changshun, additional, Chen, Hong, additional, Shi, Yufang, additional, He, Jiuyang, additional, and Kaplan, David L., additional

Published
2021
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34. Silk Nanocarrier Size Optimization for Enhanced Tumor Cell Penetration and Cytotoxicity In Vitro

Author

Xiao, Liying, Ding, Zhaozhao, Zhang, Xiaoyi, Wang, Xue, Lu, Qiang, and Kaplan, David L.

Abstract

Silk nanofibers are versatile carriers for hydrophobic and hydrophilic drugs, but fall short in terms of effective delivery to cells, which is essential for therapeutic benefits. Here, the size of silk nanofibers was tuned by ultrasonic treatment to improve the cell penetration features without impacting the structural features. The gradual decrease in silk nanofiber length from 1700 to 40 nm resulted in improved cell uptake. The internalized silk nanofiber carriers evaded lysosomes, which facilitated retention in cancer cells in vitro. The smaller sizes also facilitated enhanced penetration of tumor spheroids for improved delivery in vitro. The cytotoxicity of paclitaxel (PTX)-laden nanocarriers increased when the length of the silk nanocarriers decreased. Both the drug loading capacity and delivery of silk nanocarriers with optimized sizes suggest potential utility in cell treatments.

Published
2022
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35. Green process to prepare water-insoluble silk scaffolds with silk I structure

Author

Zhang Zhengshi, Qin Jianzhong, Shen Yixin, Zuo Bao-qi, Huang Ji-wei, Zhang Feng, and Ding Zhaozhao

Subjects
Materials science, Biocompatibility, Nanofibers, Silk, Nanotechnology, Biocompatible Materials, 02 engineering and technology, Water insoluble, 010402 general chemistry, 01 natural sciences, Biochemistry, X-Ray Diffraction, Structural Biology, Materials Testing, Spectroscopy, Fourier Transform Infrared, Molecular Biology, Mechanical Phenomena, Calorimetry, Differential Scanning, Tissue Scaffolds, fungi, technology, industry, and agriculture, General Medicine, 021001 nanoscience & nanotechnology, Porous scaffold, 0104 chemical sciences, SILK, Solubility, Nanofiber, 0210 nano-technology, Porosity
Abstract

Silk porous scaffolds have shown promising applications in tissue regenerations as cellular scaffolds to incorporate cells in vitro and in vivo, and facilitate cell proliferation and production of extracellular matrix. It remains strong needs to optimize the microstructures and performances of silk scaffolds for better biocompatibility. Here, a green process was developed to form water-insoluble scaffolds. Repeated freezing-dissolving procedures and silk nanofibers were introduced to tune the performances of the scaffolds, resulting in amorphous conformations and nanofibrous structures. Controllable degradation and mechanical properties as well as improved cell compatibility were then achieved for these scaffolds, suggesting their promising future in tissue regenerations. Our present results confirmed the possibility of actively designing silk scaffolds with preferable properties used in various tissue regenerations.

Published
2018

43. Tough Anisotropic Silk Nanofiber Hydrogels with Osteoinductive Capacity

Author

Ding, Zhaozhao, Lu, Guozhong, Cheng, Weinan, Xu, Gang, Zuo, Baoqi, Lu, Qiang, and Kaplan, David L.

Abstract

Multiple physical cues such as hierarchical microstructures, topography, and stiffness influence cell fate during tissue regeneration. Yet, introducing multiple physical cues to the same biomaterial remains a challenge. Here, a synergistic cross-linking strategy was developed to fabricate protein hydrogels with multiple physical cues based on combinations of two types of silk nanofibers. β-sheet-rich silk nanofibers (BSNFs) were blended with amorphous silk nanofibers (ASNFs) to form composite nanofiber systems. The composites were transformed into tough hydrogels through horseradish peroxidase (HRP) cross-linking in an electric field, where ASNFs were cross-linked with HRP, while BSNFs were aligned by the electrical field. Anisotropic morphologies and higher stiffness of 120 kPa were achieved. These anisotropic hydrogels induced osteogenic differentiation and the aligned aggregation of stem cells in vitro while also exhibiting osteoinductive capacity in vivo. Improved tissue outcomes with the hydrogels suggest promising applications in bone tissue engineering, as the processing strategy described here provides options to form hydrogels with multiple physical cues.

Published
2024
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44. Water‐insoluble amorphous silk fibroin scaffolds from aqueous solutions.

Author

Fan, Zhihai, Xiao, Liying, Lu, Guozhong, Ding, Zhaozhao, and Lu, Qiang

Subjects
SILK fibroin, AQUEOUS solutions, DRUG delivery devices, TEMPERATURE control, TISSUE scaffolds
Abstract

Regenerated silk fibroin (RSF) is emerging as promising biomaterial for regeneration, drug delivery and optical devices, with continued demand for mild, all‐aqueous processes to control microstructure and the performance. Here, temperature control of assembly kinetics was introduced to prepare the water‐insoluble scaffolds from neutral aqueous solutions of RSF protein. Higher temperatures were used to accelerate the assembly rate of the silk fibroin protein chains in aqueous solution and during the lyophilization process, resulting in water‐insoluble scaffold formation. The scaffolds were mainly composed of amorphous states of the silk fibroin chains, endowing softer mechanical properties. These scaffolds also showed nanofibrous structures, improved cell proliferation in vitro and enhanced neovascularization and tissue regeneration in vivo than previously reported silk fibroin scaffolds. These results suggest utility of silk scaffolds in soft tissue regeneration. [ABSTRACT FROM AUTHOR]

Published
2020
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