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Despite the high lethality of colorectal cancers (CRCs), only a limited number of genetic risk factors are identified. The mammalian ssDNA-binding protein complex CTC1-STN1-TEN1 protects genome stability, yet its role in tumorigenesis is unknown. Here, we show that attenuated CTC1/STN1 expression is common in CRCs. We generated an inducible STN1 knockout mouse model and found that STN1 deficiency in young adult mice increased CRC incidence, tumor size, and tumor load. CRC tumors exhibited enhanced proliferation, reduced apoptosis, and elevated DNA damage and replication stress. We found that STN1 deficiency down-regulated multiple DNA glycosylases, resulting in defective base excision repair (BER) and accumulation of oxidative damage. Collectively, this study identifies STN1 deficiency as a risk factor for CRC and implicates the previously unknown STN1-BER axis in protecting colon tissues from oxidative damage, therefore providing insights into the CRC tumor–suppressing mechanism.

Mixed metal oxide composites have been widely used as adsorbents for the removal of heavy metal ions from wastewater. In this work, Fe2O3/TiO2 composite was sustainably prepared via the treatment of titanium slag with a low-concentration sulfuric acid solution (20%) and used for the removal of As(V) from aqueous solutions. The resulting products were characterized by X-ray diffraction (XRD), N2 adsorption−desorption, Fourier-transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), and energy-dispersive X-ray spectroscopy (EDX). The batch adsorption was employed to investigate the removal efficiency of the Fe2O3/TiO2 adsorbent toward As(V). The Langmuir and Freundlich isotherms were plotted in order to study the adsorption process. The adsorption of As(V) on FeO3/TiO2 fitted well with the Freundlich isotherm model, suggesting a multilayer adsorption process with an adsorption capacity of 68.26 mg·g−1. The adsorption kinetics study demonstrated that the adsorption behavior of the Fe2O3/TiO2 composite for the As(V) was pseudo-second-order. With low-cost preparation and high adsorption capacity, the prepared Fe2O3/TiO2 adsorbent could be used as an effective adsorbent for As(V) removal from contaminated water sources. The approach utilized in this research is viewed as a sustainable route for creating a proficient adsorbent for the purification of water.

Colon tissues and tumors were collected at 34 weeks and embedded in paraffin, and genomic DNA was extracted and sent for WES sequencing. Results are from 3 WT and 3 STN1F/F mice, with normal and tumor tissue adjacent.

Replication stress causes replication fork stalling, resulting in an accumulation of single-stranded DNA (ssDNA). Replication protein A (RPA) and CTC1-STN1-TEN1 (CST) complex bind ssDNA and are found at stalled forks, where they regulate RAD51 recruitment and foci formation in vivo. Here, we investigate crosstalk between RPA, CST, and RAD51. We show that CST and RPA localize in close proximity in cells. Although CST stably binds to ssDNA with a high affinity at low ionic strength, the interaction becomes more dynamic and enables facilitated dissociation at high ionic strength. CST can coexist with RPA on the same ssDNA and target RAD51 to RPA-coated ssDNA. Notably, whereas RPA-coated ssDNA inhibits RAD51 activity, RAD51 can assemble a functional filament and exhibit strand-exchange activity on CST-coated ssDNA at high ionic strength. Our findings provide mechanistic insights into how CST targets and tethers RAD51 to RPA-coated ssDNA in response to replication stress., During replication stress, the RPA protein complex coats single-stranded DNA to preclude RAD51 loading. Here, the authors show how RPA and CST crosstalk to regulate RAD51 activity.

Synthesizing novel photocatalysts that can effectively harvest photon energy over a wide range of the solar spectrum for practical applications is vital. Porphyrin-derived nanostructures with properties similar to those of chlorophyll have emerged as promising candidates to meet this requirement. In this study, tetrakis(4-carboxyphenyl) porphyrin (TCPP) nanofibers were formed on the surface of ZnO nanoparticles using a simple self-assembly approach. The obtained ZnO/TCPP nanofiber composites were characterized via scanning electron microscopy, X-ray diffraction analysis, and ultraviolet–visible absorbance and reflectance measurements. The results demonstrated that the ZnO nanoparticles with an average size of approximately 37 nm were well integrated in the TCPP nanofiber matrix. The resultant composite showed photocatalytic activity of ZnO and TCPP nanofibers concomitantly, with band gap energies of 3.12 and 2.43 eV, respectively. The ZnO/TCPP photocatalyst exhibited remarkable photocatalytic performance for RhB degradation with a removal percentage of 97% after 180 min of irradiation under simulated sunlight because of the synergetic activity of ZnO and TCPP nanofibers. The dominant active species participating in the photocatalytic reaction were •O2– and OH•, resulting in enhanced charge separation by exciton-coupled charge-transfer processes between the hybrid materials.

Sewage sludge (SS) is increasingly used as an environment functional material to reduce or control pollution and improve plant growth because of the large amounts of carbon and essential plant nutrients in it. To achieve the best application results, it is essential to comprehensively review recent progress in SS utilization. This review aims to fill the gaps in knowledge by describing the properties of SS, and its usage as adsorbents, catalysts and fertilizers, and certain application mechanisms. Although SS generates several benefits for the environment and humans, many challenges still exist to limit the application, including the risks posed by potentially toxic substances (e.g., heavy metals) in SS. Therefore, future research directions are discussed and how to make SS applications more feasible in terms of technology and economy.

Three types of biomass of invasive plants and agrowastes, namely, the wattle bark of Acacia auriculiformis (BA), mimosa (BM), and coffee husks (BC), were converted into biochars through slow pyrolysis and investigated for their ability to remove dyes in water. The properties of the materials were characterized using Fourier transform infrared (FTIR) spectroscopy, scanning electron microscopy (SEM), and Brunauer–Emmett–Teller (BET) analysis. The BET surface area (total pore volume) of BC was 2.62 m2/g (0.007 cm3/g), far below those of BA and BM with 393.15 cm2/g (0.195 m3/g) and 285.53 cm2/g (0.153 m3/g), respectively. The optimal adsorption doses for the removal of methylene blue (MB) were found to be 2, 5, and 5 g/L for BC, BA, and BM, respectively. The suitable pH ranges for MB removal were 6–12 for BA, 7–12 for BC, and 2–10 for BM. The majority of MB (over 83%) was removed in the initial 30 min, followed by a more quasisteady state condition after the removal rate exceeded 90%. The experimental data were fitted with the kinetic models (PFO, PSO, Bangham, IDP), indicating that physicochemical adsorption, pore diffusion process, and multiple stages are the dominant mechanisms for the MB adsorption onto biochars. Finally, BA and BM showed similar adsorption efficiencies, while BC may not be favorable for use as an adsorbent due to its low surface area and low pore volume.

This present research investigates, simulates, and models the transport ethane fluid in different phases. The applied technical, operational, and technological facilities, limitations, and economic estimation have been in various PVT thermodynamic conditions according to several states of fluid that will directly affect the transport volume. In these plans, different limitations of loading, storing, transporting, and unloading have been evaluated to transport the gaseous or liquid ethane. The simulators such as Aspen HYSY, Refprop, Aspen Capital Cost Estimator and Pipesim have been used for the assessment of processing, the thermodynamic, economical, loading, and unloading conditions. The results indicated that the mixture of fluid transport in the gaseous and compressed phase will be less costly. The best condition of transporting is almost 80 bar pressure and the temperature of 40 °C. The cost of loading, storing, transporting, and unloading has been 135 dollars/ton.

The demethanizer tower of Kavian olefin plant had a problem in more than 77% of the nominal capacity. Its performance was reduced by flooding with considering the inside pressure drop and fluctuation of the bottom liquid level increase. For this reason, a study was performed and the simulation results (with PRO II and SULCOL) were successfully implemented. Finally, troubleshooting and practical solutions were compared with simulation results. In order to tower modifications, an instruction had been issued so that all 40 trays had to be completely discharged and then the tower was refilled with 16 ton packing. According to this solution, the volume of ethylene as final product was increases about 23% and reaches the designed nominal capacity.

The CO2 methanation reaction of reduced and unreduced Ni based CeO2, Al2O3, TiO2 and Y2O3 supported catalysts was investigated. The Ni/CeO2 and Ni/Y2O3 catalysts exhibited similar CO2 conversions at all reaction temperatures. The catalysts were studied by X-ray diffraction (XRD), H2 chemisorption, H2 temperature-programmed reduction (TPR), and in situ diffuse reflection infrared Fourier transform spectroscopy (DRIFTS); the results suggested that the reducibility of both metal and support at low temperature, strong metal support interaction and small Ni particle size are important factors for low-temperature CO2 methanation. Based on the DRIFT studies, the difference in the CO2 adsorption properties and reaction pathway depending on the reduced and unreduced Ni based supported catalysts was discussed.

The main objective of this study was to banana waste-derived activated carbon (BWAC) make a high pore surface area was prepared and composited with Fe

Clean energy like hydrogen can be a promising strategy to solve problems of global warming. Photo-fermentation (PF) is an attractive technology for producing biohydrogen from various biowastes cost-effectively and environmentally friendly. However, challenges of low light conversion efficiency and small yields of biohydrogen production still limit its application. Thus, advanced strategies have been investigated to enhance photo-fermentative biohydrogen production. This review discusses advanced technologies that show positive outcomes in improving biohydrogen production by PF, including the following. Firstly, genetic engineering enhances light transfer efficiency, change the activity of enzymes, and improves the content of ATP, ammonium and antibiotic tolerance of photosynthetic bacteria. Secondly, immobilization technology is refined. Thirdly, nanotechnology makes great strides as a scientific technique and fourthly, integration of dark and photo-fermentation technology is possible. Some suggestions for further studies to achieve high levels of efficiency of photo-fermentative biohydrogen production are mentioned in this paper.

The present study aimed to evaluate the effects of chrysoeriol from Cardiospermum halicacabum in streptozotocin induced Wistar rats. Thirty rats were categorized as control, diabetic control supplemented with 0, 20 mg/kg chrysoeriol and 600 μg/kg BW of glibenclamide for 45-day trial period. Our results indicated that the inclusion of chrysoeriol (20 mg/kg), showed a significant reduction in plasma glucose, hemoglobin and glycosylated hemoglobin level with a rising of plasma insulin sensitivity. Further, downregulated enzymes including glucose 6-phosphatase, fructose 1,6-bisphosphatase, and glycogen phosphorylase as well upregulated enzymes such as hexokinase, glucose-6-phosphate dehydrogenase, pyruvate kinase, and hepatic glycogen content. There was a diminish action found in liver glycogen synthase of tested rat with a rise in gamma-glutamyl transpeptidase, towards normal levels upon treatment with chrysoeriol. The histopathological study confirmed that renewal of the beta cells of pancreatic of chrysoeriol and glibenclamide treated rats. In addition, the molecular docking of chrysoeriol against glycolytic enzymes including hexokinase, glucose-6-phosphate dehydrogenase, pyruvate kinase, using Argus software shows chrysoeriol had greatest ligand binding energy as equivalent to glibenclamide, as a standard drug. Thus, chrysoeriol found to be non-toxic with potential regulation on glycemic control and upregulation of the carbohydrate metabolic enzymes.

This study examines the use of international publishing (i.e. publishing in journals/books indexed by international bibliometrics databases) within academia in Vietnam since Doi Moi (the Renovation) in 1986. Document analysis and in‐depth interviews with 20 Vietnamese scholars were undertaken to address the research objective. The findings revealed three important milestones in academic development in Vietnam: 1986–2008, 2008–2017, and 2017 to the present. Between 1986 and 2008, international publishing was almost non‐existent in actual practice in academia, except among the mathematics community. Since 2008, with the launch of the Foundation for Science and Technology Development, international publishing in Vietnam has become more visible on a broader scope (i.e. at ministerial level). Increasing awareness in the academic community of the role of international publishing contributed to a major increase in research output with an international outlook between 2008 and 2017. The third milestone was the promulgation of two new regulations, one relating to doctoral education in 2017 and another to professorship appointments in 2018, in which international publishing databases have been placed at the centre of these policy agendas. Looking ahead, policymakers and academic leaders in Vietnam are recommended to further use international publishing metrics to enhance international research outputs and quality.

The failure of the centralized water supply system forced XY community to become more dependent on uncertain and unstable water sources. The results of surveying 50 households showed that 89.18% of total households depended on water collected from rivers, which contributed 58.3% of the total water volume used for the domestic demands. The average water volume consumed was 19.5 liters/person/day (l/p/d), and 86.5% of households used more than one source; 13.5% of households collected water only from rivers, and 45.94% of families had rainwater harvesting (RWH) for their activities (domestic water demand); however, RWH only provided 9.9% of total water consumption. In this study, basic methods were applied to calculate the storage tanks necessary to balance the water deficit created by drought months. Three levels of water demand (14, 20, and 30 l/p/d) can be the best choices for RWH; for a higher demand (40 and 60 l/p/d), small roof area (30-40 m2), and many people (six to seven) per family, RWH might be impractical because of unsuitable rainfall or excessively large storage tanks.

The primary goal of the current study is thermodynamic and environmental modeling and multi-objective optimization of a new hybrid energy system. The suggested ocean-based energy conversion system consists of an organic Rankine cycle (ORC), a solar flat plate collector, a proton exchange membrane (PEM) electrolyzer boosted with thermoelectric generator (TEG) module. Exergy and exergo-environmental as two powerful tools are employed to the precise assessment of the suggested system. To achieve the best performance of the integrated system, multi-criteria optimization with different objective functions is carried out. As the results of the parametric analysis indicate, defined objective goals have an appropriate conflict with changing decision variables, which is necessary in multi-criteria optimization. Four decision variables namely solar flat plate collector area ( A p ), solar radiation intensity (I), collector temperature ( T col ), and condenser temperature ( T cond ) are chosen as decision variables. In all optimization scenarios, the net output power is common, which is based on the selected optimum points value changing between 201.02 kW and 268.17 kW. Additionally, in various optimization scenarios, the area of solar flat plate collectors tends to get a higher value in the allowable domain. The provided information in multi-objective optimization can provide good insight to engineers and system designers to select the best system configuration.

The current work is about analysis and multi-objective optimization (MOO) of weir-type solar still systems equipped with phase change material (PCM) regarding the exergetic and economic performance. To do so, the energetic and exergetic modeling of the suggested system is conducted then the substantial economic factors is applied to obtain the total cost rate of the considered SSDS. The total exergetic efficiency and total annual cost (TAC) is considered objective functions. Four parameters include mass of the PCM (mPCM), inlet brine water flow rate ( $$ {\dot{m}}_{\mathrm{f}} $$ ), gap distance (d), and insulation width (xins) is chosen as decision variables. Moreover, a genetic algorithm–based MOO was applied to find the optimum states of evaluated solar still unit. The outputs represented that increasing the brine feed water mass flow rate does not affect the TAC while decreasing distilled water production rate. The scattered distribution of optimum states infers that the optimum value of PCM mass is about 1 kg. In addition, applied MOO reveals that with optimization of the studied system, the exergy efficiency increases about 1.47% and the annual distilled water increases 4.35% compared with the non-optimized system. The suggested system is capable to produce fresh water in remote areas without any pollution as well as in a low cost rate.