412 results on '"Disorders of the Nervous System"'
Search Results
2. Jean-René Cruchet (1875–1959)
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Walusinski, Olivier
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- 2024
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3. Jean-Martin Charcot (1825–1893)
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Walusinski, Olivier
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- 2024
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4. BDNF-Induced Plasticity of Spinal Circuits Underlying Pain and Learning
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Garraway, Sandra M.
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- 2023
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5. Nicotinic Acetylcholine Receptors and Affective Responses
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Schulz, Kalynn, Chavez, Marcia, and Castaneda, Arthur
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- 2022
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6. Nociceptors and Chronic Pain
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Walters, Edgar T.
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- 2021
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7. The Role of Neuroinflammation in the Response to Spinal Cord Injury
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Bodart, Olivia H., Glaser, Ethan P., MacLean, Steven M., Chen, Meifan A., and Gensel, John C.
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- 2021
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8. Regulating Systems in Neuroimmunology
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Walker II, William H. and DeVries, A. Courtney
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- 2021
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9. Phantom Limbs and Brain Plasticity in Amputees
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Makin, Tamar and Plasticity Lab, London
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- 2020
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10. Aging and Olfaction
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Doty, Richard L.
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- 2020
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11. Somatosensory System Organization in Mammals and Response to Spinal Injury
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Darian-Smith, Corinna and Fisher, Karen
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- 2019
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12. Achieving Adaptive Plasticity in the Spinal Cord
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Grau, James W.
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- 2019
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13. Role of Sex Hormones on Pain
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Averitt, Dayna L., Hornung, Rebecca S., and Murphy, Anne Z.
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- 2019
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14. Gut Dysbiosis and Recovery of Function After Spinal Cord Injury
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Kigerl, Kristina A. and Popovich, Phillip G.
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- 2019
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15. The Hypocretin Arousal Network
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Borniger, Jeremy C. and de Lecea, Luis
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- 2019
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16. Enhancing the Regeneration of Neurons in the Central Nervous System
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Cartoni, Romain, Bradke, Frank, and He, Zhigang
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- 2019
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17. Mechanisms of Behavioral Changes After Spinal Cord Injury
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Fouad, Karim, Torres-Espín, Abel, and Fenrich, Keith K.
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- 2019
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18. Neuroscience Insights
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nervous system structure ,disorders of the nervous system ,neuropharmacology ,cognition ,Neurosciences. Biological psychiatry. Neuropsychiatry ,RC321-571 - Published
- 2020
19. Using Neural Stem Cells to Enhance Repair and Recovery of Spinal Circuits After Injury
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Fischer, Itzhak and Hou, Shaoping
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- 2018
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20. Neuroimmunology: Behavioral Effects
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Neigh, Gretchen N., Bekhbat, Mandakh, and Rowson, Sydney A.
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- 2018
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21. Developmental Neurobiology of Anxiety and Related Disorders
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Cohodes, Emily M. and Gee, Dylan G.
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- 2017
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22. Relevancia de componentes del eje hipotálamo-hipófisis-gonadal en la fisiopatología de proteinopatías del sistema nervioso.
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Hechavarría Barzaga, Kenia, Aguilera Rodríguez, Raúl, Almaguer Gotay, Dennis, Álvarez Sosa, Amarilis, and Almaguer Mederos, Luis Enrique
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Copyright of Revista Habanera de Ciencias Médicas is the property of Universidad de Ciencias Medicas de La Habana and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
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- 2019
23. Metformin Reduces Repeat Mild Concussive Injury Pathophysiology
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Erica L. Underwood, John B. Redell, Mark E. Maynard, Nobuhide Kobori, Michael J. Hylin, Kimberly N. Hood, Rebecca K. West, Jing Zhao, Anthony N. Moore, and Pramod K. Dash
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Male ,General Neuroscience ,Brain ,General Medicine ,Metformin ,axonal injury ,Mice, Inbred C57BL ,Disease Models, Animal ,Mice ,cognitive dysfunction ,oxygen consumption rate ,mild traumatic brain injury ,Head Injuries, Closed ,repeat concussion ,tissue respiration ,Animals ,Disorders of the Nervous System ,Research Article: New Research ,Brain Concussion - Abstract
Mild traumatic brain injury (mTBI) can initiate complex pathophysiological changes in the brain. Numerous cellular and molecular mechanisms underlying these pathologic changes are altered after injury, including those involved in energy utilization, excitotoxicity, ionic disturbances, and inflammation. It is thought that targeting multiple mechanisms may be necessary to produce meaningful reductions in brain pathology and to improve outcome. Previous studies have reported that the anti-diabetic drug metformin can also affect inflammatory, cell survival, and metabolic outcomes, possibly by acting on multiple targets and/or pathways. We therefore questioned whether metformin treatment can reduce pathology after repeat mild closed head injury (rmCHI) in male C57Bl/6 mice. We found that metformin, administered acutely after each head impact, resulted in markedly reduced white matter damage, astrogliosis, loss of hippocampal parvalbumin neurons, and improved mitochondrial function. In addition, both motor and cognitive functions were significantly improved when tested after discontinuation of the treatment. These studies suggest that metformin may be beneficial as a treatment for repeat concussion.
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- 2022
24. Protein Profiling of RGS6, a Pleiotropic Gene Implicated in Numerous Neuropsychiatric Disorders, Reveals Multi-Isoformic Expression and a Novel Brain-Specific Isoform
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K. E. Ahlers-Dannen, J. Yang, M. M. Spicer, B. Maity, A. Stewart, J. G. Koland, and R. A. Fisher
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pleiotropic ,General Neuroscience ,isoforms ,Brain ,Genetic Pleiotropy ,General Medicine ,splicing ,Mice ,GTP-Binding Proteins ,neuropsychiatric ,expression ,Animals ,Protein Isoforms ,Disorders of the Nervous System ,RGS6 ,Research Article: New Research ,RGS Proteins - Abstract
A metanalysis identified regulator of G-protein signaling 6 (RGS6) as one of 23 loci with pleiotropic effects on four or more human psychiatric disorders. This finding is significant as it confirms/extends the findings of numerous other studies implicating RGS6 in CNS function and pathology. RGS6 is a highly conserved member of the RGS protein family whose cellular roles are likely affected by mRNA splicing and alternative domain inclusion/exclusion. Indeed, we previously identified multiple RGS6 splice variants predicted to produce 36 distinct protein isoforms containing either long (RGS6L) or short (RGS6S) N-terminal domains, an incomplete or intact GGL domain, and nine alternative C termini. Unfortunately, sequence similarities between the isoforms have made it difficult to confirm their individual existence and/or to determine their unique functions. Here, we developed three RGS6-specific antibodies that recognize all RGS6 protein isoforms (RGS6-fl), the N-terminus of RGS6L isoforms (RGS6-L), and an 18-amino acid alternate C-terminal sequence (RGS6-18). Using these antibodies, we demonstrate that RGS6L(+GGL) isoforms, predominating in both mouse (both sexes) CNS and peripheral tissues, are most highly expressed in the CNS. We further identify three novel RGS6 protein bands that are larger (61, 65, and 69-kDa) than the ubiquitously expressed 53- to 57-kDa RGS6L(+GGL) proteins. Importantly, we show that the 69-kDa protein is a brain-specific dephospho form of the 65-kDa band, the first identified phosphorylated RGS6 isoform. Together, these data begin to define the functional significance behind the complexity ofRGS6gene processing and further clarifies RGS6’s physiological roles by resolving tissue-specific RGS6 protein expression.
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- 2022
25. Disruption of VGLUT1 in Cholinergic Medial Habenula Projections Increases Nicotine Self-Administration
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Elizabeth A. Souter, Yen-Chu Chen, Vivien Zell, Valeria Lallai, Thomas Steinkellner, William S. Conrad, William Wisden, Kenneth D. Harris, Christie D. Fowler, and Thomas S. Hnasko
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Habenula ,Nicotine ,Tobacco Smoke and Health ,General Neuroscience ,Interpeduncular Nucleus ,Substance Abuse ,Neurosciences ,glutamate ,medial habenula ,General Medicine ,Fluorescence ,acetylcholine ,Brain Disorders ,Mice ,Tobacco ,Animals ,Disorders of the Nervous System ,Nicotinic Agonists ,corelease ,In Situ Hybridization ,Research Article: New Research ,In Situ Hybridization, Fluorescence - Abstract
Cholinergic projections from the medial habenula (MHb) to the interpeduncular nucleus (IPN) have been studied for their complex contributions to nicotine addiction and have been implicated in nicotine reinforcement, aversion, and withdrawal. While it has been established that MHb cholinergic projections corelease glutamate, no direct evidence has demonstrated a role for this glutamate projection in nicotine consumption. In the present study, a novel floxedSlc17a7[vesicular glutamate transporter 1 (VGLUT1)] mouse was generated and used to create conditional knock-out (cKO) mice that lack VGLUT1 in MHb cholinergic neurons. Loss ofSlc17a7expression in ventral MHb cholinergic neurons was validated using fluorescentin situhybridization, and immunohistochemistry was used to demonstrate a corresponding reduction of VGLUT1 protein in cholinergic terminals in the IPN. We also used optogenetics-assisted electrophysiology to evoke EPSCs in IPN and observed a reduction of glutamatergic currents in the cKO, supporting the functional disruption of VGLUT1 in MHb to IPN synapses. cKO mice exhibited no gross phenotypic abnormalities and displayed normal thigmotaxis and locomotor behavior in the open-field assay. When trained to lever press for food, there was no difference between control and cKO. However, when tested in a nicotine self-administration procedure, we found that the loss of VGLUT1-mediated glutamate corelease led to increased responding for nicotine. These findings indicate that glutamate corelease from ventral MHb cholinergic neurons opposes nicotine self-administration, and provide additional support for targeting this synapse to develop potential treatments for nicotine addiction.
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- 2022
26. Dysregulated mRNA Translation in the G2019S LRRK2 and LRRK2 Knock-Out Mouse Brains
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Jungwoo Wren Kim, Yulan Xiong, Stephen M. Eacker, Valina L. Dawson, Xiling Yin, Ted M. Dawson, Ian Martin, and Nicholas T. Ingolia
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Untranslated region ,Induced Pluripotent Stem Cells ,RPS15 ,translation ,Substantia nigra ,Mice, Transgenic ,Biology ,Leucine-Rich Repeat Serine-Threonine Protein Kinase-2 ,Mice ,Eukaryotic translation ,Translational regulation ,Animals ,Humans ,Ribosome profiling ,Kinase activity ,Mice, Knockout ,General Neuroscience ,Brain ,Translation (biology) ,LRRK2 ,Parkinson Disease ,General Medicine ,nervous system diseases ,Cell biology ,Protein Biosynthesis ,Disorders of the Nervous System ,Research Article: New Research - Abstract
Visual Abstract, The G2019S mutation in leucine-rich repeat kinase 2 (LRRK2) causes familial Parkinson’s disease (PD) and is also found in a subset of idiopathic cases. Prior studies in Drosophila and human induced pluripotent stem cell (iPSC)-derived dopamine neurons uncovered a pronounced effect of G2019S LRRK2 on mRNA translation. It was previously reported that G2019S LRRK2 promotes translation of mRNAs with complex 5′ untranslated region (UTR) secondary structure, resulting in increased expression of calcium channels and dysregulated calcium homeostasis in human dopamine neurons. Here, we show that dysregulated translation occurs in the brains of mammalian LRRK2 models in vivo. Through ribosome profiling studies of global translation, we observe that mRNAs with complex 5′UTR structure are also preferentially translated in the G2019S LRRK2-expressing mouse brain. Reporter assays suggest that this 5′UTR preference is independent of translation initiation factors. Conversely, translation of mRNAs with complex 5′UTR secondary structure is downregulated in LRRK2 knock-out (KO) mouse brain, indicating a robust link between LRRK2 kinase activity and translation of mRNA with complex 5′UTR structure. Further, substantia nigra pars compacta (SNpc) dopamine neurons in the G2019S LRRK2-expressing brain exhibit increased calcium influx, which is consistent with the previous report from human dopamine neurons. These results collectively suggest that LRRK2 plays a mechanistic role in translational regulation, and the G2019S mutation in LRRK2 causes translational defects leading to calcium dysregulation in the mammalian brain.
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- 2021
27. Timing of Splenectomy after Acute Spinal Cord Injury
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Feng Wu, Xiao-Hui Li, Min-Jie Gong, Jia-Qi An, Xiao-Yan Ding, and Sheng-Li Huang
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Tumor Necrosis Factor-alpha ,General Neuroscience ,acute ,General Medicine ,Recovery of Function ,spinal cord injury ,splenectomy ,Rats ,Rats, Sprague-Dawley ,Spinal Cord ,timing ,Animals ,Disorders of the Nervous System ,Spinal Cord Injuries ,Research Article: New Research - Abstract
Spinal cord injury (SCI) is a devastating condition. Splenectomy may play a protective role in the development of SCI. However, little is known about whether the timing of splenectomy affects the outcome after SCI. Investigation into splenectomy after SCI would provide insight into how the timing can be selected following SCI to improve neurologic outcomes. Rats were randomized into a sham group, a nonsplenectomized group (NonSPX), four splenectomized groups with the surgery performed immediately, 6 h, 12 h, and 24 h after SCI (SPX0, SPX6, SPX12, and SPX24, respectively). Rats were subjected to severe contusive SCI at the level of the third thoracic vertebra. At different time points following SCI, Basso, Beattie, and Bresnahan (BBB) score was used to assess the recovery of injury. The animals in each group were randomly selected for tissue collection at days 3, 14, and 28 after surgery. Then, immunohistochemistry of immunologic cells was performed and inflammatory mediators were determined. Our study showed that splenectomy within 6 h after SCI improved BBB scores as compared with splenectomy more than 12 h after SCI, and decrease the immune cell responses to SCI. Protein levels of interleukin (IL)-1β and tumor necrosis factor (TNF)-α were significantly elevated in nonsplenectomized group compared with sham group. No difference was observed in IL-10 at the lesion site between splenectomized and nonsplenectomized groups at 3 d post-SCI. The study demonstrates that splenectomy within 6 h after SCI would lessen the development of SCI and improve outcome.
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- 2021
28. Intrauterine Growth Restriction Causes Abnormal Embryonic Dentate Gyrus Neurogenesis in Mouse Offspring That Leads to Adult Learning and Memory Deficits
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Jill Chang, Raul Chavez-Valdez, Ashley S. Brown, Camille Fung, Maria L.V. Dizon, Shelby Murdock, Richard I. Dorsky, Matthew Wieben, and Mark St. Pierre
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Male ,intrauterine growth restriction ,Neurogenesis ,hypertensive disease of pregnancy ,Hippocampal formation ,Hippocampus ,fetal growth restriction ,neural stem cell ,SOX2 ,Neural Stem Cells ,Pregnancy ,medicine ,Humans ,reproductive and urinary physiology ,embryonic dentate gyrus neurogenesis ,Memory Disorders ,Fetal Growth Retardation ,biology ,General Neuroscience ,Dentate gyrus ,Wnt signaling pathway ,General Medicine ,Neural stem cell ,Doublecortin ,medicine.anatomical_structure ,Dentate Gyrus ,biology.protein ,Disorders of the Nervous System ,Female ,Neuron ,learning and memory ,Neuroscience ,Research Article: New Research - Abstract
Human infants who suffer from intrauterine growth restriction (IUGR), which is a failure to attain their genetically pre-determined weight, are at increased risk for postnatal learning and memory deficits. Hippocampal dentate gyrus (DG) granule neurons play an important role in memory formation, however it is unknown whether IUGR affects embryonic DG neurogenesis, which could provide a potential mechanism underlying abnormal postnatal learning and memory function. Using a mouse model of the most common cause of IUGR, induced by hypertensive disease of pregnancy, we first assessed adult learning and memory function. We quantified the percentages of embryonic hippocampal DG neural stem and progenitor cells and developing glutamatergic granule neurons, as well as hippocampal volumes and neuron cell count and morphology 18 and 40 days after delivery. We characterized the differential embryonic hippocampal transcriptomic pathways between appropriately-grown and IUGR mouse offspring. We found that IUGR offspring of both sexes had short-term adult learning and memory deficits. Prenatally, we found that IUGR caused accelerated embryonic DG neurogenesis and Sox2+ neural stem cell depletion. IUGR mice were marked by decreased hippocampal volumes and decreased doublecortin+ neuronal progenitors with increased mean dendritic lengths at postnatal day (P) 18. Consistent with its known molecular role in embryonic DG neurogenesis, we also found evidence for decreased Wnt pathway activity during IUGR. In conclusion, we have discovered that postnatal memory deficits are associated with accelerated NSC differentiation and maturation into glutamatergic granule neurons following IUGR, a phenotype that could be explained by decreased embryonic Wnt signaling.Significance StatementPostnatal learning and memory deficits are common in infants born with IUGR. However, the embryonic cellular and molecular hippocampal changes are elusive compared to the many postnatal studies in the literature. Using a translationally relevant mouse model with short-term memory deficits, we discovered that IUGR accelerated embryonic hippocampal DG neurogenesis along with NSC depletion. Decreased postnatal hippocampal volumes with altered neuronal progenitor development were noted. Transcriptomic analysis revealed decreased Wnt signaling as a strong candidate for embryonic cellular changes after IUGR. To our knowledge, this is the first investigation linking IUGR-induced embryonic hippocampal DG alterations with postnatal neuronal developmental aberrations. This model provides a framework to investigate other hippocampal domains and cell types that modulate neuronal maturation and memory function.
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- 2021
29. Altered Cerebellar Response to Somatosensory Stimuli in the Cntnap2 Mouse Model of Autism
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Javier Márquez-Ruiz, Marta Fernández, Javier Llorente, Teresa Sierra-Arregui, Olga Peñagarikano, Carlos A. Sánchez-León, Shira Knafo, Ministerio de Ciencia, Innovación y Universidades (España), Agencia Estatal de Investigación (España), European Commission, Universidad del País Vasco, Israel Science Foundation, and Ministerio de Economía y Competitividad (España)
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CNTNAP2 ,Cerebellum ,Sensory processing ,cerebellum ,medicine.medical_treatment ,autism ,Sensory system ,Stimulation ,Nerve Tissue Proteins ,Biology ,Somatosensory system ,Mice ,Purkinje Cells ,sensory stimuli ,cntnap2 ,medicine ,Animals ,complex spike ,Autistic Disorder ,Mice, Knockout ,Purkinje ,Cerebellar ataxia ,General Neuroscience ,Membrane Proteins ,General Medicine ,medicine.disease ,medicine.anatomical_structure ,Vibrissae ,Autism ,Disorders of the Nervous System ,medicine.symptom ,Neuroscience ,Research Article: New Research - Abstract
Atypical sensory processing is currently included within the diagnostic criteria of autism. The cerebellum is known to integrate sensory inputs of different modalities through its connectivity to the cerebral cortex. Interestingly, cerebellar malformations are among the most replicated features found in postmortem brain of individuals with autism. We studied sensory processing in the cerebellum in a mouse model of autism, knock-out (KO) for the Cntnap2 gene. Cntnap2 is widely expressed in Purkinje cells (PCs) and has been recently reported to regulate their morphology. Further, individuals with CNTNAP2 mutations display cerebellar malformations and CNTNAP2 antibodies are associated with a mild form of cerebellar ataxia. Previous studies in the Cntnap2 mouse model show an altered cerebellar sensory learning. However, a physiological analysis of cerebellar function has not been performed yet. We studied sensory evoked potentials in cerebellar Crus I/II region on electrical stimulation of the whisker pad in alert mice and found striking differences between wild-type and Cntnap2 KO mice. In addition, single-cell recordings identified alterations in both sensory-evoked and spontaneous firing patterns of PCs. These changes were accompanied by altered intrinsic properties and morphologic features of these neurons. Together, these results indicate that the Cntnap2 mouse model could provide novel insight into the pathophysiological mechanisms of autism core sensory deficits., This work was supported by the Spanish Ministry of Science (MCIU/AEl/FEDER) Grant RTI2018-101427-B-I00 (to O.P.), the ERANET-NEURON Grant nEUrotalk (to O.P.), the University of the Basque Country (UPV/EHU) Grant GIU18/094 (to O.P.), the Israel Science Foundation Grant 536/19 (to S.K.), the Spanish Ministry of Science Grant SAF2016-78071-R (to S.K.), and the Spanish Ministry of Economy (MINECO-FEDER) Grant BFU2017-89615-P (to J.M.-R.). M.F. holds the MINECO Predoctoral Fellowship BES-2016–078420, and T.S.-A. is a Basque Government predoctoral fellow (PRE-2020–2-0109).
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- 2021
30. The Type 2 Diabetes Factor Methylglyoxal Mediates Axon Initial Segment Shortening and Alters Neuronal Function at the Cellular and Network Levels
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Ryan B. Griggs, John A. Miller, Josef K. Steinbrunner, Leonid M. Yermakov, Carlos Gonzalez-Islas, Keiichiro Susuki, Peter Wenner, Jennae N. Shelby, Jeneane M. Jaber, and Duc V. M. Nguyen
- Subjects
Male ,medicine.medical_specialty ,multielectrode array ,medicine.medical_treatment ,axon initial segment ,Diabetes Mellitus, Experimental ,chemistry.chemical_compound ,Mice ,depolarization ,Internal medicine ,medicine ,methylglyoxal ,Animals ,Axon ,Membrane potential ,Neurons ,Chemistry ,General Neuroscience ,Insulin ,Methylglyoxal ,Depolarization ,General Medicine ,network activity ,Pyruvaldehyde ,Axon initial segment ,Endocrinology ,medicine.anatomical_structure ,Diabetes Mellitus, Type 2 ,Tetrodotoxin ,Excitatory postsynaptic potential ,Disorders of the Nervous System ,Female ,type 2 diabetes ,Research Article: New Research - Abstract
Recent evidence suggests that alteration of axon initial segment (AIS) geometry (i.e., length or location along the axon) contributes to CNS dysfunction in neurological diseases. For example, AIS length is shorter in the prefrontal cortex of type 2 diabetic mice with cognitive impairment. To determine the key type 2 diabetes-related factor that produces AIS shortening we modified levels of insulin, glucose, or the reactive glucose metabolite methylglyoxal in cultures of dissociated cortices from male and female mice and quantified AIS geometry using immunofluorescent imaging of the AIS proteins AnkyrinG and βIV spectrin. Neither insulin nor glucose modification altered AIS length. Exposure to 100 but not 1 or 10 μmmethylglyoxal for 24 h resulted in accumulation of the methylglyoxal-derived advanced glycation end-product hydroimidazolone and produced reversible AIS shortening without cell death. Methylglyoxal-evoked AIS shortening occurred in both excitatory and putative inhibitory neuron populations and in the presence of tetrodotoxin (TTX). In single-cell recordings resting membrane potential was depolarized at 0.5–3 h and returned to normal at 24 h. In multielectrode array (MEA) recordings methylglyoxal produced an immediate ∼300% increase in spiking and bursting rates that returned to normal within 2 min, followed by a ∼20% reduction of network activity at 0.5–3 h and restoration of activity to baseline levels at 24 h. AIS length was unchanged at 0.5–3 h despite the presence of depolarization and network activity reduction. Nevertheless, these results suggest that methylglyoxal could be a key mediator of AIS shortening and disruptor of neuronal function during type 2 diabetes.
- Published
- 2021
31. Long-Term Potentiation of Mossy Fiber Feedforward Inhibition of CA3 Pyramidal Cells Maintains E/I Balance in Epilepsy Model
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Enhui Pan, Ram S. Puranam, and James O. McNamara
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status epilepticus ,Epilepsy ,CA3 pyramidal cells ,General Neuroscience ,Pyramidal Cells ,Long-Term Potentiation ,homeostatic ,General Medicine ,Mice ,nervous system ,Epilepsy, Temporal Lobe ,mossy fiber ,Mossy Fibers, Hippocampal ,Animals ,Disorders of the Nervous System ,feedforward inhibition ,Research Article: New Research - Abstract
Insight into the cellular and circuit mechanisms underlying development of temporal lobe epilepsy (TLE) will provide a foundation for improved therapies. We studied a model in which an episode of prolonged seizures is followed by recovery lasting two weeks before emergence of spontaneous recurrent seizures. We focused on the interval between the prolonged seizures and the late onset recurrent seizures. We investigated the hippocampal mossy fiber CA3 pyramidal cell microcircuit in models spanningin vitro,in vivo, andex vivopreparations. Expression of channelrhodopsin-2 in the dentate granule cells ofDGC ChRmice enabled the selective activation of mossy fiber axons.In vivostudies revealed marked potentiation of mossy fiber evoked field potentials in hippocampal CA3 beginning within hours following seizures, a potentiation which persisted at least 7 d. Stimulation of mossy fibers in hippocampal slicesin vitrousing patterns of activity mimicking seizures induced LTP not only of the monosynaptic EPSC but also of the disynaptic IPSC of CA3 pyramidal cells.Ex vivostudies of slices isolated following seizures revealed evidence of LTP of mossy fiber evoked EPSC and disynaptic IPSC of CA3 pyramidal cells. We suggest that activation of dentate granule cells during seizures induces these plasticitiesin vivoand the retained balance of synaptic excitation and inhibition limits excessive activation of CA3 pyramidal cells, thereby protecting animals from spontaneous recurrent seizures at this interval following status epilepticus.
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- 2021
32. Modeling Neurodevelopmental Disorders and Epilepsy Caused by Loss of Function of kif2a in Zebrafish
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Michèle Partoens, Hoi-Khoanh Giong, Jeong-Soo Lee, Ann-Sofie De Meulemeester, Aleksandra Siekierska, Peter de Witte, and Duc-Hung Pham
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Microcephaly ,Kinesins ,Epilepsy ,Mice ,drug-resistant epilepsy ,Tubulin ,Intellectual Disability ,Intellectual disability ,medicine ,Animals ,Habituation ,Zebrafish ,Pathological ,Loss function ,seizures ,biology ,General Neuroscience ,General Medicine ,malformations of cortical development ,biology.organism_classification ,medicine.disease ,zebrafish ,Phenotype ,Repressor Proteins ,Disorders of the Nervous System ,Neuroscience ,Research Article: New Research ,KIF2A - Abstract
Visual Abstract, In recent years there has been extensive research on malformations of cortical development (MCDs) that result in clinical features like developmental delay, intellectual disability, and drug-resistant epilepsy (DRE). Various studies highlighted the contribution of microtubule-associated genes (including tubulin and kinesin encoding genes) in MCD development. It has been reported that de novo mutations in KIF2A, a member of the kinesin-13 family, are linked to brain malformations and DRE. Although it is known that KIF2A functions by regulating microtubule depolymerization via an ATP-driven process, in vivo implications of KIF2A loss of function remain partly unclear. Here, we present a novel kif2a knock-out zebrafish model, showing hypoactivity, habituation deficits, pentylenetetrazole-induced seizure susceptibility and microcephaly, as well as neuronal cell proliferation defects and increased apoptosis. Interestingly, kif2a−/− larvae survived until adulthood and were fertile. Notably, our kif2a zebrafish knock-out model demonstrated many phenotypic similarities to KIF2A mouse models. This study provides valuable insights into the functional importance of kif2a in zebrafish and phenotypical hallmarks related to KIF2A mutations. Ultimately, this model could be used in a future search for more effective therapies that alleviate the clinical symptoms typically associated with MCDs.
- Published
- 2021
33. IgM Immunoglobulin Influences Recovery after Cervical Spinal Cord Injury by Modulating the IgG Autoantibody Response
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Antigona Ulndreaj, Nicole Forgione, Pia M. Vidal, Michael G. Fehlings, and James Hong
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functional recovery ,Lesion ,White matter ,Myelin ,Mice ,medicine ,Animals ,IgG-autoantibody response ,Spinal cord injury ,Spinal Cord Injuries ,Autoantibodies ,Microglia ,biology ,business.industry ,General Neuroscience ,IgM immunoglobulin ,Autoantibody ,Cervical Cord ,General Medicine ,Recovery of Function ,Spinal cord ,medicine.disease ,spinal cord injury ,medicine.anatomical_structure ,Immunoglobulin M ,Spinal Cord ,Immunoglobulin G ,Immunology ,biology.protein ,Disorders of the Nervous System ,medicine.symptom ,Antibody ,business ,Research Article: New Research - Abstract
Spinal cord injury (SCI) results in the development of detrimental autoantibodies against the lesioned spinal cord. IgM immunoglobulin maintains homeostasis against IgG-autoantibody responses, but its effect on SCI recovery remains unknown. In the present study we investigated the role of IgM immunoglobulin in influencing recovery after SCI. To this end, we induced cervical SCI at the C6/C7 level in mice that lacked secreted IgM immunoglobulin (IgM-KO) and their wild type (WT) littermate controls. Overall, the absence of secretory IgM resulted in worse outcomes as compared to WT mice with SCI. At two weeks post injury, IgM knock-out (KO) mice had significantly more IgG antibodies, which fixed the complement system, in the injured spinal cord parenchyma. In addition to these findings, IgM-KO mice had more parenchymal T-lymphocytes as well as CD11b+ microglia/macrophages, which co-localized with myelin. At 10 weeks post-injury, IgM-KO mice showed significant impairment in neurobehavioral recovery, such as deteriorated coordination, reduced hindlimb swing speed and print area. These neurobehavioral detriments were coupled with increased lesional tissue and myelin loss. Taken together, this study provides the first evidence for the importance of IgM immunoglobulin in modulating recovery after SCI and suggests that modulating IgM could be a novel therapeutic approach to enhance recovery after SCI. Significance statement The present study provides novel evidence for the protective role of IgM immunoglobulin in SCI. Using a clinically relevant mouse model of SCI at the cervical level (C6/C7), we show that deficiency of IgM immunoglobulin results in impaired neurobehavioral recovery, coupled with increased lesion size, less white matter sparing, and enhanced deposition of complement-fixing IgG antibodies in the spinal cord. These data provide evidence for the necessary role of IgM immunoglobulin in spontaneous recovery during cervical SCI and warrant more research into the therapeutic effect of IgM administration after SCI.
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- 2021
34. Knock-Down of Heterogeneous Nuclear Ribonucleoprotein A1 Results in Neurite Damage, Altered Stress Granule Biology, and Cellular Toxicity in Differentiated Neuronal Cells
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Catherine Hutchinson, Patricia A. Thibault, Hannah E. Salapa, S Austin Hammond, Amber Anees, and Michael C. Levin
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Small interfering RNA ,Neurite ,viruses ,Heterogeneous Nuclear Ribonucleoprotein A1 ,genetic processes ,RNA-binding protein ,Biology ,environment and public health ,neuronal cell damage ,Cell Line ,Mice ,Stress granule ,neurodegenerative disease ,Gene expression ,Neurites ,Animals ,Neurons ,Neuro-2a cell line ,Gene knockdown ,General Neuroscience ,RNA ,General Medicine ,RNA binding protein ,small interfering RNA ,Stress Granules ,Cell biology ,Gene Knockdown Techniques ,health occupations ,Disorders of the Nervous System ,Research Article: New Research - Abstract
Heterogeneous nuclear ribonucleoprotein A1 (hnRNP A1) is an RNA binding protein (RBP) that is localized within neurons and plays crucial roles in RNA metabolism. Its importance in neuronal functioning is underscored from the study of its pathogenic features in many neurodegenerative diseases where neuronal hnRNP A1 is mislocalized from the nucleus to the cytoplasm resulting in loss of hnRNP A1 function. Here, we model hnRNP A1 loss-of-function by siRNA mediated knockdown in differentiated Neuro-2a cells. Through RNA sequencing (RNA-seq) followed by gene ontology (GO) analyses, we show that hnRNP A1 is involved in important biological processes, including RNA metabolism, neuronal function, neuronal morphology, neuronal viability, and stress granule (SG) formation. We further confirmed several of these roles by showing that hnRNP A1 knockdown results in a reduction of neurite outgrowth, increase in cell cytotoxicity and changes in SG formation. In summary, these findings indicate that hnRNP A1 loss-of-function contributes to neuronal dysfunction and cell death and implicates hnRNP A1 dysfunction in the pathogenesis of neurodegenerative diseases.Significance StatementHnRNP A1 plays a biologically important role in controlling gene expression and maintaining proper cellular functioning in neurons. Previous research has shown that many neurodegenerative diseases exhibit pathogenic features of hnRNP A1 dysfunction, whereby it is mislocalized from its homeostatic nuclear location to the cytoplasm resulting in loss of proper functioning. Here, we model hnRNP A1 loss-of-function in differentiated neuronal cells and show that it contributes to neuronal dysfunction and cell death. These data are important because it underscores the importance of loss-of-function models and implicates hnRNP A1 dysfunction in the pathogenesis of neurodegenerative diseases.
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- 2021
35. Beneficial Effects of Transplanted Human Bone Marrow Endothelial Progenitors on Functional and Cellular Components of Blood-Spinal Cord Barrier in ALS Mice
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Cesario V. Borlongan, Surafuale Hailu, Robert Shell, Jared Ehrhart, Paul R. Sanberg, Hilmi Mustafa, Svitlana Garbuzova-Davis, Kayla J. Boccio, Stanley H. Appel, and Alexander Llauget
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Pathology ,medicine.medical_specialty ,Endothelium ,CD34 ,Mice, Transgenic ,Mice ,G93A SOD1 mice ,Bone Marrow ,medicine ,human bone marrow-derived stem cells ,Animals ,Humans ,Progenitor cell ,Basement membrane ,Tight junction ,business.industry ,Superoxide Dismutase ,General Neuroscience ,Amyotrophic Lateral Sclerosis ,Endothelial Cells ,General Medicine ,Transplantation ,Disease Models, Animal ,medicine.anatomical_structure ,Spinal Cord ,blood-spinal cord barrier ,repair ,Disorders of the Nervous System ,Pericyte ,Stem cell ,ALS ,business ,Research Article: New Research ,transplantation - Abstract
Convincing evidence of blood-spinal cord barrier (BSCB) alterations has been demonstrated in amyotrophic lateral sclerosis (ALS) and barrier repair is imperative to prevent motor neuron dysfunction. We showed benefits of human bone marrow-derived CD34+ cells (hBM34+) and endothelial progenitor cells (hBM-EPCs) intravenous transplantation into symptomatic G93A SOD1 mutant mice on barrier reparative processes. These gains likely occurred by replacement of damaged endothelial cells, prolonging motor neuron survival. However, additional investigations are needed to confirm the effects of administered cells on integrity of the microvascular endothelium. The aim of this study was to determine tight junction protein levels, capillary pericyte coverage, microvascular basement membrane, and endothelial F-actin status in spinal cord capillaries of G93A SOD1 mutant mice treated with human bone marrow-derived stem cells. Tight junction proteins were detected in the spinal cords of cell-treated vs. non-treated mice via western blotting at four weeks post-transplant. Capillary pericyte, basement membrane laminin, and endothelial F-actin magnitudes were determined in cervical/lumbar spinal cord tissues in ALS mice, including controls, by immunohistochemistry and fluorescent staining. Results showed that cell-treated vs. media-treated ALS mice substantially increased tight junction protein levels, capillary pericyte coverage, basement membrane laminin immunoexpressions, and endothelial cytoskeletal F-actin fluorescent expressions. The greatest benefits were detected in mice receiving hBM-EPCs vs. hBM34+ cells. These study results support treatment with a specific cell type derived from human bone marrow towards BSCB repair in ALS. Thus, hBM-EPCs may be advanced for clinical applications as a cell-specific approach for ALS therapy through restored barrier integrity. Significance Statement Repairing the disease-altered blood-spinal cord barrier (BSCB) in ALS via cell transplantation may be a feasible therapeutic approach. We showed benefits of intravenously transplanted human bone marrow-derived stem cells into symptomatic G93A SOD1 mutant mice on barrier reparative processes, likely by replacing damaged endothelial cells. However, effects of administered cells on endothelium integrity in mouse CNS capillaries was not fully determined. Here we showed that ALS mice receiving human bone marrow endothelial progenitor cells (hBM-EPCs) vs. hBM34+ cells substantially increased tight junction protein levels, capillary pericyte coverage, basement membrane laminin immunoexpressions, and endothelial cytoskeletal F-actin fluorescent expressions. These results provide evidence that hBM-EPCs effectively maintained capillary endothelium integrity in ALS and these cells may be advanced for clinical applications restoring BSCB integrity.
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- 2021
36. Lack of Hyperinhibition of Oriens Lacunosum-Moleculare Cells by Vasoactive Intestinal Peptide-Expressing Cells in a Model of Temporal Lobe Epilepsy
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Paul S. Buckmaster and Megan Wyeth
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Interneuron ,OLM ,Postsynaptic Current ,hippocampus ,Vasoactive intestinal peptide ,interneuron ,Biology ,Miniature Postsynaptic Potentials ,Inhibitory postsynaptic potential ,CA1 ,Temporal lobe ,Epilepsy ,Mice ,Interneurons ,medicine ,Hippocampus (mythology) ,Animals ,General Neuroscience ,Pyramidal Cells ,CCK ,General Medicine ,medicine.disease ,Rats ,VIP ,medicine.anatomical_structure ,nervous system ,Epilepsy, Temporal Lobe ,Disorders of the Nervous System ,Neuroscience ,Research Article: New Research ,Vasoactive Intestinal Peptide - Abstract
Temporal lobe epilepsy remains a common disorder with no cure and inadequate treatments, potentially due to an incomplete understanding of how seizures start. CA1 pyramidal cells and many inhibitory interneurons increase their firing rate in the seconds-minutes before a spontaneous seizure in epileptic rats. However, some interneurons fail to do so, including those identified as putative interneurons with somata in oriens and axons targeting lacunosum-moleculare (OLM cells). Somatostatin-containing cells, including OLM cells, are the primary target of inhibitory vasoactive intestinal polypeptide and calretinin-expressing (VIP/CR) bipolar interneuron-selective interneurons, type 3 (ISI-3). The objective of this study was to test the hypothesis that in epilepsy inhibition of OLM cells by ISI-3 is abnormally increased, potentially explaining the failure of OLM recruitment when needed most during the ramp up of activity preceding a seizure. Stereological quantification of VIP/CR cells in a model of temporal lobe epilepsy demonstrated that they survive in epileptic mice, despite a reduction in their somatostatin-expressing cell targets. Paired recordings of unitary inhibitory postsynaptic currents from ISI-3 to OLM cells did not show increased connection probability or increased connection strength, and failure rate was unchanged. When miniature postsynaptic currents in ISI-3 were compared, only mIPSC frequency was increased in epileptic hippocampi. Nevertheless, spontaneous and miniature postsynaptic potentials were unchanged in OLM cells of epileptic mice. These results are not consistent with the hypothesis of hyper-inhibition from VIP/CR bipolar cells impeding recruitment of OLM cells in advance of a seizure. Significance Inadequate recruitment of inhibitory cells in general, and OLM cells in particular, may be a mechanism of seizure initiation, making it important to determine why OLM cells do not fire faster and provide preictal feedback inhibition when presynaptic CA1 pyramidal activity is ramping up. This study excludes aberrantly increased inhibition of OLM cells by VIP bipolar cells as the cause, pointing to other possibilities for investigation.
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- 2021
37. Deciphering Spinal Endogenous Dopaminergic Mechanisms That Modulate Micturition Reflexes in Rats with Spinal Cord Injury
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Chuanxi Tang, William C. de Groat, Jaclyn H DeFinis, Shaoping Hou, Stephanie L. Daugherty, and Jeremy Weinberger
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Agonist ,medicine.drug_class ,media_common.quotation_subject ,Urinary Bladder ,Urination ,Pharmacology ,Rats, Sprague-Dawley ,Quinpirole ,medicine ,detrusor-sphincter dyssynergia ,dopamine receptor ,Tonic (music) ,Animals ,Spinal cord injury ,Spinal Cord Injuries ,media_common ,business.industry ,Electromyography ,General Neuroscience ,Urethral sphincter ,General Medicine ,Spinal cord ,medicine.disease ,tonic activity ,Rats ,Apomorphine ,Urodynamics ,medicine.anatomical_structure ,Spinal Cord ,Disorders of the Nervous System ,Female ,bladder overactivity ,bursting ,business ,Research Article: New Research ,medicine.drug - Abstract
Visual Abstract, Spinal neuronal mechanisms regulate recovered involuntary micturition after spinal cord injury (SCI). It was recently discovered that dopamine (DA) is synthesized in the rat injured spinal cord and is involved in lower urinary tract (LUT) activity. To fully understand the role of spinal DAergic machinery in micturition, we examined urodynamic responses in female rats during pharmacological modulation of the DA pathway. Three to four weeks after complete thoracic SCI, the DA precursor L-DOPA administered intravenously during bladder cystometrogram (CMG) and external urethral sphincter (EUS) electromyography (EMG) reduced bladder overactivity and increased the duration of EUS bursting, leading to remarkably improved voiding efficiency. Apomorphine (APO), a non-selective DA receptor (DR) agonist, or quinpirole, a selective DR2 agonist, induced similar responses, whereas a specific DR2 antagonist remoxipride alone had only minimal effects. Meanwhile, administration of SCH 23390, a DR1 antagonist, reduced voiding efficiency by increasing tonic EUS activity and shortening the EUS bursting period. Unexpectedly, SKF 38393, a selective DR1 agonist, increased EUS tonic activity, implying a complicated role of DR1 in LUT function. In metabolic cage assays, subcutaneous administration of quinpirole decreased spontaneous voiding frequency and increased voiding volume; L-DOPA and APO were inactive possibly because of slow entry into the CNS. Collectively, tonically active DR1 in SCI rats inhibit urine storage and enhance voiding by differentially modulating EUS tonic and bursting patterns, respectively, while pharmacologic activation of DR2, which are normally silent, improves voiding by enhancing EUS bursting. Thus, enhancing DA signaling achieves better detrusor-sphincter coordination to facilitate micturition function in SCI rats.
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- 2021
38. Enhanced Synaptic Transmission in the Extended Amygdala and Altered Excitability in an Extended Amygdala to Brainstem Circuit in a Dravet Syndrome Mouse Model
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Alyssa Levitt, Maya Xia, Dane M. Chetkovich, Jennifer A. Kearney, William P. Nobis, Wen Wei Yan, Nicole A. Hawkins, Jeremy Chiang, and Geoffrey T. Swanson
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Epilepsies, Myoclonic ,AMPA receptor ,Biology ,Neurotransmission ,Sudden death ,Synaptic Transmission ,Mice ,extended amygdala ,Extended amygdala ,Neurotransmitter receptor ,bed nucleus of the stria terminalis ,excitability ,Animals ,parabrachial nucleus ,General Neuroscience ,General Medicine ,Amygdala ,Dravet syndrome ,Mice, Inbred C57BL ,NAV1.1 Voltage-Gated Sodium Channel ,Electrophysiology ,Stria terminalis ,Disorders of the Nervous System ,Brainstem ,Neuroscience ,Research Article: New Research - Abstract
Visual Abstract, Dravet syndrome (DS) is a developmental and epileptic encephalopathy with an increased incidence of sudden death. Evidence of interictal breathing deficits in DS suggests that alterations in subcortical projections to brainstem nuclei may exist, which might be driving comorbidities in DS. The aim of this study was to determine whether a subcortical structure, the bed nucleus of the stria terminalis (BNST) in the extended amygdala, is activated by seizures, exhibits changes in excitability, and expresses any alterations in neurons projecting to a brainstem nucleus associated with respiration, stress response, and homeostasis. Experiments were conducted using F1 mice generated by breeding 129.Scn1a+/− mice with wild-type C57BL/6J mice. Immunohistochemistry was performed to quantify neuronal c-fos activation in DS mice after observed spontaneous seizures. Whole-cell patch-clamp and current-clamp electrophysiology recordings were conducted to evaluate changes in intrinsic and synaptic excitability in the BNST. Spontaneous seizures in DS mice significantly enhanced neuronal c-fos expression in the BNST. Further, the BNST had altered AMPA/NMDA postsynaptic receptor composition and showed changes in spontaneous neurotransmission, with greater excitation and decreased inhibition. BNST to parabrachial nucleus (PBN) projection neurons exhibited intrinsic excitability in wild-type mice, while these projection neurons were hypoexcitable in DS mice. The findings suggest that there is altered excitability in neurons of the BNST, including BNST-to-PBN projection neurons, in DS mice. These alterations could potentially be driving comorbid aspects of DS outside of seizures, including respiratory dysfunction and sudden death.
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- 2021
39. Stress Controllability Modulates Basal Activity of Dopamine Neurons in the Substantia Nigra Compacta
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Zhijun Diao, Yongfeng Li, Qiaohua Zheng, Chunling Wei, Yuanyuan Di, Meilin Wu, Wei Ren, Li Yao, Zhiqiang Liu, Juan Fan, Jing Han, Yingfang Tian, and Zhaoqiang Qian
- Subjects
Dopamine ,Stress ,controllability ,Stress (mechanics) ,chemistry.chemical_compound ,Basal (phylogenetics) ,Mice ,Reward ,Corticosterone ,substantia nigra compacta ,medicine ,Animals ,Chronic stress ,Pars Compacta ,Pars compacta ,General Neuroscience ,Dopaminergic Neurons ,General Medicine ,Controllability ,basal activity ,chemistry ,nervous system ,Shock (circulatory) ,Disorders of the Nervous System ,medicine.symptom ,Neuroscience ,Research Article: New Research ,medicine.drug - Abstract
Prolonged stress induces neural maladaptations in mesolimbic dopamine (DA) system and produces emotional and behavioral disorders. However, the effects of stress on activity of DA neurons are diverse and complex that hinge on the type, duration, intensity, and controllability of stressors. Here, controlling the duration, intensity, and type of the stressors to be identical, we observed effects of stressor controllability on the activity of substantia nigra compacta (SNc) DA neurons in mice. We found that both lack and loss of control over shock enhance the basal activity and intrinsic excitability of SNc DA neurons via modulation of Ih current, but not via corticosterone serum level. Moreover, loss of control over shock produces more significant enhancement in the basal activity of SNc DA neurons than that produced shock per se, and therefore attenuates the response to natural reward. This attenuation can be reversed by control over shock. These results indicate that although chronic stress per se tends to enhance the basal activity of SNc DA neurons, loss of control over the stressor is able to induce a larger enhancement in basal activity of SNc DA neurons and produce more severe behavioral deficits. However, control over stress ameliorates the deleterious effects of stress, highlighting the role of stress controllability.Significance statementThe impact of stress on the DA system significantly modifies immediate and guide future behaviors. Stress does not have unitary effects on VTA DA neurons, but the effects of stress controllability on SNc DA neurons are unclear. The present work studied the effects of controllability on the activity of SNc DA neurons by controlling the duration, intensity, and pattern of footshocks to be identical. The results show that loss of control over shock produces larger enhancement in basal activity of SNc DA neurons and worse behavioral deficits than what caused by uncontrollable shock per se. The results demonstrate the critical role of stress controllability in modulating activity of SNc DA neurons and inducing behavioral deficits.
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- 2021
40. Intranasal Administration of Oxytocin Attenuates Social Recognition Deficits and Increases Prefrontal Cortex Inhibitory Postsynaptic Currents following Traumatic Brain Injury
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Ramesh Raghupathi, Avery Runyan, Jessica R. Barson, Jimmy W. Huh, and Dana Lengel
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Adult ,Male ,Traumatic brain injury ,Postsynaptic Current ,Prefrontal Cortex ,Inhibitory postsynaptic potential ,Oxytocin ,intranasal administration ,social behavior ,excitability ,Brain Injuries, Traumatic ,Medicine ,pediatric TBI ,Animals ,Humans ,GABAergic neurotransmission ,Prefrontal cortex ,Child ,Administration, Intranasal ,business.industry ,General Neuroscience ,Novelty ,General Medicine ,medicine.disease ,Oxytocin receptor ,Rats ,nervous system ,Inhibitory Postsynaptic Potentials ,Hypothalamus ,Disorders of the Nervous System ,Female ,business ,Neuroscience ,Research Article: New Research ,medicine.drug - Abstract
Pediatric traumatic brain injury (TBI) results in heightened risk for social deficits that can emerge during adolescence and adulthood. A moderate TBI in male and female rats on postnatal day 11 (equivalent to children below the age of 4) resulted in impairments in social novelty recognition, defined as the preference for interacting with a novel rat compared with a familiar rat, but not sociability, defined as the preference for interacting with a rat compared with an object in the three-chamber test when tested at four weeks (adolescence) and eight weeks (adulthood) postinjury. The deficits in social recognition were not accompanied by deficits in novel object recognition memory and were associated with a decrease in the frequency of spontaneous inhibitory postsynaptic currents (IPSCs) recorded from pyramidal neurons within Layer II/III of the medial prefrontal cortex (mPFC). Whereas TBI did not affect the expression of oxytocin (OXT) or the OXT receptor (OXTR) mRNAs in the hypothalamus and mPFC, respectively, intranasal administration of OXT before behavioral testing was found to reduce impairments in social novelty recognition and increase IPSC frequency in the mPFC in brain-injured animals. These results suggest that TBI-induced deficits in social behavior may be linked to increased excitability of neurons in the mPFC and suggests that the regulation of GABAergic neurotransmission in this region as a potential mechanism underlying these deficits.
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- 2021
41. Isoform-Specific Reduction of the Basic Helix-Loop-Helix Transcription Factor TCF4 Levels in Huntington's Disease
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Jürgen Tuvikene, Mari Sepp, Kaja Nurm, Hanna Vihma, Tõnis Timmusk, Jordi Creus-Muncunill, Alex Sirp, Carla Castany-Pladevall, Derek J. Blake, and Esther Pérez-Navarro
- Subjects
Gene isoform ,Adult ,Male ,basic helix-loop-helix transcription factor ,Hippocampus ,Mice, Transgenic ,Biology ,Huntington's chorea ,Mice ,Transcripció genètica ,Transcription Factor 4 ,neurodegenerative disease ,Huntington's disease ,Corea de Huntington ,medicine ,Animals ,Humans ,Protein Isoforms ,transcriptional regulation ,Transcription factor ,TCF4 ,Neurons ,Genetic transcription ,General Neuroscience ,Neurogenesis ,General Medicine ,medicine.disease ,ASCL1 ,Disease Models, Animal ,medicine.anatomical_structure ,Huntington Disease ,Cerebral cortex ,Disorders of the Nervous System ,Neuroscience ,Research Article: New Research ,Huntington’s disease - Abstract
Huntington’s disease (HD) is an inherited neurodegenerative disorder with onset of characteristic motor symptoms at midlife, preceded by subtle cognitive and behavioral disturbances. Transcriptional dysregulation emerges early in the disease course and is considered central to HD pathogenesis. Using wild-type and HD knock-in mouse striatal cell lines we observed a HD genotype-dependent reduction in the protein levels of transcription factor 4 (TCF4), a member of the basic helix-loop-helix family with critical roles in brain development and function. We characterized mouse Tcf4 gene structure and expression of alternative mRNAs and protein isoforms in cell-based models of HD, and in four different brain regions of male transgenic HD mice (R6/1) from young to mature adulthood. The largest decrease in the levels of TCF4 at mRNA and specific protein isoforms were detected in the R6/1 mouse hippocampus. Translating this finding to human disease, we found reduced expression of long TCF4 isoforms in the post-mortem hippocampal CA1 area and in the cerebral cortex of HD patients. Additionally, TCF4 protein isoforms showed differential synergism with the proneural transcription factor ASCL1 in activating reporter gene transcription in hippocampal and cortical cultured neurons. Induction of neuronal activity increased these synergistic effects in hippocampal but not in cortical neurons, suggesting brain region-dependent differences in TCF4 functions. Collectively, this study demonstrates isoform-specific changes in TCF4 expression in HD that could contribute to the progressive impairment of transcriptional regulation and neuronal function in this disease. Significance Statement Historically, HD has been considered a neurodegenerative disease. However, research of the last decade has revealed disrupted neurogenesis and cognitive dysfunction preceding pathological neuronal cell death, suggesting that HD is also a neurodevelopmental disease. One of the major molecular mechanisms of HD is dysregulation of transcription. Studying transcription factors with functions in neurogenesis and neural plasticity is of interest for their potential participation in the cognitive impairment in HD etiology. Here we show reduced expression of the transcription factor TCF4, previously linked with neurodevelopmental and neuropsychiatric diseases, in hippocampus and cerebral cortex of R6/1 mouse and HD patients. Our results shed light on the potential neurodevelopmental aspect of HD, and could be applicable for developing alleviating therapies for HD.
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- 2021
42. Single Dose of Amphetamine Induces Delayed Subregional Attenuation of Cholinergic Interneuron Activity in the Striatum
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Sixtine Fleury, Nao Chuhma, Samira Ztaou, Miriam Matamales, Stephen Rayport, Sophia Tepler, Jesus Bertran-Gonzalez, and Soo Jung Oh
- Subjects
Interneuron ,Dopamine ,Cholinergic Agents ,psychostimulant ,Striatum ,Nucleus accumbens ,Nucleus Accumbens ,Mice ,fluorescence imaging ,Interneurons ,medicine ,Animals ,Amphetamine ,Chemistry ,General Neuroscience ,Ventral striatum ,General Medicine ,acetylcholine ,phosphorylated ribosomal protein S6 ,medicine.anatomical_structure ,nervous system ,Cholinergic ,Disorders of the Nervous System ,Neuron ,Neuroscience ,Research Article: New Research ,medicine.drug - Abstract
Psychostimulants such as amphetamine target dopamine neuron synapses to engender drug-induced plasticity. While dopamine neurons modulate the activity of striatal cholinergic interneurons (ChIs) with regional heterogeneity, how amphetamine affects ChI activity has not been elucidated. Here, we applied quantitative fluorescence imaging approaches to map the dose-dependent effects of a single dose of amphetamine on ChI activity at 2.5 and 24 hours after injection across the mouse striatum using the activity-dependent marker phosphorylated ribosomal protein S6 (p-rpS6240/244). Amphetamine did not affect the distribution or morphology of ChIs in any striatal subregion. While amphetamine at either dose had no effect on ChI activity after 2.5 hours, ChI activity was dose-dependently reduced after 24 hours specifically in the ventral striatum/nucleus accumbens, a critical site of psychostimulant action. Amphetamine at either dose did not affect the spontaneous firing of ChIs. Altogether this work demonstrates that a single dose of amphetamine has delayed regionally heterogeneous effects on ChI activity, which most likely involves extra-striatal synaptic input. Significance statement Using the activity dependent marker phosphorylated ribosomal protein S6 (p-rpS6240/244), we mapped amphetamine effects on the activity of cholinergic interneurons (ChIs) across the striatum. Amphetamine reduced ChI activity in dose-dependent manner in the ventral striatum/nucleus accumbens, a critical site of psychostimulant action.
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- 2021
43. CaMKII Phosphorylation Regulates Synaptic Enrichment of Shank3
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Jaehoon Jeong, Katherine W. Roche, and Yan Li
- Subjects
Scaffold protein ,inorganic chemicals ,Autism Spectrum Disorder ,autism ,Nerve Tissue Proteins ,environment and public health ,Serine ,Ca2+/calmodulin-dependent protein kinase ,Humans ,posttranslational modification ,CaMKII ,Chemistry ,phosphorylation ,General Neuroscience ,Alternative splicing ,Colocalization ,General Medicine ,Cell biology ,enzymes and coenzymes (carbohydrates) ,nervous system ,Shank3 ,Synapses ,Excitatory postsynaptic potential ,Phosphorylation ,bacteria ,Disorders of the Nervous System ,Calcium-Calmodulin-Dependent Protein Kinase Type 2 ,Postsynaptic density ,Research Article: New Research - Abstract
SHANK3 is a large scaffolding protein in the postsynaptic density (PSD) that organizes protein networks, which are critical for synaptic structure and function. The strong genetic association ofSHANK3with autism spectrum disorder (ASD) emphasizes the importance of SHANK3 in neuronal development. SHANK3 has a critical role in organizing excitatory synapses and is tightly regulated by alternative splicing and posttranslational modifications. In this study, we examined basal and activity-dependent phosphorylation of Shank3 using mass spectrometry (MS) analysis fromin vitrophosphorylation assays,in situexperiments, and studies with cultured neurons. We found that Shank3 is highly phosphorylated, and we identified serine 782 (S782) as a potent CaMKII phosphorylation site. Using a phosphorylation state-specific antibody, we demonstrate that CaMKII can phosphorylate Shank3 S782in vitroand in heterologous cells on cotransfection with CaMKII. We also observed an effect of a nearby ASD-associated variant (Shank3 S685I), which increased S782 phosphorylation. Notably, eliminating phosphorylation of Shank3 with a S782A mutation increased Shank3 and PSD-95 synaptic puncta size without affecting Shank3 colocalization with PSD-95 in cultured hippocampal neurons. Taken together, our study revealed that CaMKII phosphorylates Shank3 S782 and that the phosphorylation affects Shank3 synaptic properties.
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- 2021
44. Phenotypic Differences between the Alzheimer’s Disease-Related hAPP-J20 Model and Heterozygous Zbtb20 Knock-Out Mice
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Xinxing Yu, Gui-Qiu Yu, Daniel Kim, Melanie Das, Eric Shao, Kaitlyn Ho, Weiping J. Zhang, Lennart Mucke, Daniel R. Gulbranson, Reuben Thomas, and Krishna Choudhary
- Subjects
Genetically modified mouse ,Aging ,Knockout ,mouse model ,Mice, Transgenic ,amyloid precursor protein ,Biology ,Neurodegenerative ,Alzheimer's Disease ,Transgenic ,Pathogenesis ,Amyloid beta-Protein Precursor ,Mice ,Alzheimer Disease ,Amyloid precursor protein ,Genetics ,Acquired Cognitive Impairment ,2.1 Biological and endogenous factors ,Animals ,Allele ,Gene ,Mice, Knockout ,Amyloid beta-Peptides ,Animal ,behavior ,General Neuroscience ,Neurosciences ,Alzheimer's Disease including Alzheimer's Disease Related Dementias (AD/ADRD) ,General Medicine ,Phenotype ,Brain Disorders ,Disease Models, Animal ,Knockout mouse ,Disease Models ,Neurological ,biology.protein ,epilepsy ,Dementia ,Disorders of the Nervous System ,Zbtb20 ,Neural development ,Alzheimer’s disease ,Research Article: New Research ,Biotechnology ,Transcription Factors - Abstract
Diverse gene products contribute to the pathogenesis of Alzheimer’s disease (AD). Experimental models have helped elucidate their mechanisms and impact on brain functions. Human amyloid precursor protein (hAPP) transgenic mice from line J20 (hAPP-J20 mice) are widely used to simulate key aspects of AD. However, they also carry an insertional mutation in noncoding sequence of one Zbtb20 allele, a gene involved in neural development. We demonstrate that heterozygous hAPP-J20 mice have reduced Zbtb20 expression in some AD-relevant brain regions, but not others, and that Zbtb20 levels are higher in hAPP-J20 mice than heterozygous Zbtb20 knockout (Zbtb20+/–) mice. Whereas hAPP-J20 mice have premature mortality, severe deficits in learning and memory, other behavioral alterations, and prominent nonconvulsive epileptiform activity, Zbtb20+/– mice do not. Thus, the insertional mutation in hAPP-J20 mice does not ablate the affected Zbtb20 allele and is unlikely to account for the AD-like phenotype of this model. Significance Statement Genetically modified mice can help unravel complex disorders such as Alzheimer’s disease (AD) by revealing effects of pathogenic drivers on neural networks and behaviors. Inadvertent genome modifications can occur during the generation of such models but their consequences are rarely explored in depth, even though they could confound the interpretation of phenotypes and therapeutic interventions. hAPP-J20 mice simulate multiple aspects of AD but also carry an insertional mutation in one Zbtb20 allele. Our study differentiates specific from nonspecific Zbtb20 antibodies and provides evidence that the functional AD-like alterations of hAPP-J20 mice are not caused by hypofunction of Zbtb20. We further demonstrate in Zbtb20+/– mice that neural development and brain functions are well preserved when Zbtb20 levels are reduced in half.
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- 2021
45. HIV-1 Tat and Morphine Differentially Disrupt Pyramidal Cell Structure and Function and Spatial Learning in Hippocampal Area CA1: Continuous versus Interrupted Morphine Exposure
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Virginia D. McLane, Viktor Yarotskyy, Sara R. Nass, Pamela E. Knapp, Jingli Zhang, Aaron J. Barbour, A. Rory McQuiston, Kurt F. Hauser, Jean Moon, William D. Marks, Jason J. Paris, Arianna R.S. Lark, and Valerie J. Carpenter
- Subjects
Genetically modified mouse ,Spatial Learning ,Hippocampus ,Striatum ,Hippocampal formation ,hippocampal CA1 pyramidal neurons ,medicine ,neuroadaptation to opioid exposure ,CA1 Region, Hippocampal ,drug abuse ,neuro-acquired human immunodeficiency syndrome (neuroHIV) ,Morphine ,Chemistry ,synaptodendritic degeneration and dysfunction ,General Neuroscience ,Pyramidal Cells ,General Medicine ,medicine.anatomical_structure ,nervous system ,Opioid ,HIV-1 ,Trans-Activators ,Disorders of the Nervous System ,tat Gene Products, Human Immunodeficiency Virus ,Pyramidal cell ,learning and memory ,Neuroscience ,Ex vivo ,Research Article: New Research ,medicine.drug - Abstract
About half the people infected with HIV have neurocognitive deficits that often include memory impairment and hippocampal deficits, which can be exacerbated by opioid abuse. To explore the effects of opioids and HIV on hippocampal CA1 pyramidal neuron structure and function, we induced HIV-1 Tat expression in transgenic mice for 14 d and co-administered time-release morphine or vehicle subcutaneous implants during the final 5 d (days 9-14) to establish steady-state morphine levels. Morphine was withheld from some ex vivo slices during recordings to begin to assess the initial pharmacokinetic consequences of opioid withdrawal. Tat expression reduced hippocampal CA1 pyramidal neuronal excitability at lower stimulating currents. Pyramidal cell firing rates were unaffected by continuous morphine exposure. Behaviorally, exposure to Tat or high dosages of morphine impaired spatial memory Exposure to Tat and steady-state levels of morphine appeared to have largely independent effects on pyramidal neuron structure and function, a response that is distinct from other vulnerable brain regions such as the striatum. By contrast, acutely withholding morphine (from morphine-tolerant ex vivo slices) revealed unique and selective neuroadaptive shifts in CA1 pyramidal neuronal excitability and dendritic plasticity, including some interactions with Tat. Collectively, the results show that opioid-HIV interactions in hippocampal area CA1 are more nuanced than previously assumed, and appear to vary depending on the outcome assessed and on the pharmacokinetics of morphine exposure. Significance Statement HIV-1 transgenic mice were co-exposed to Tat and morphine to explore opioid-HIV interactions in hippocampal area CA1. Spatial memory was impaired by both Tat and morphine. Tat expression reduced the firing rate of hippocampal CA1 pyramidal neurons at lower stimulating currents irrespective of morphine exposure. Exposure to Tat and steady-state levels of morphine acted in a largely independent manner to alter pyramidal neuron structure, function, and associated behavior. This makes CA1 distinct from other regions such as the striatum. Alternatively, withholding morphine (from morphine-tolerant ex vivo slices) revealed unique, but subtle, neuroadaptive shifts in pyramidal neuronal excitability and dendritic plasticity, suggesting that opioid-HIV interactions in the hippocampus are markedly influenced by the pharmacokinetics of opioid exposure.
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- 2021
46. Hyperexcitability and Loss of Feedforward Inhibition Contribute to Aberrant Plasticity in the Fmr1KO Amygdala
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J. Keenan Kushner, Christian A. Cea-Del Rio, Diego Restrepo, Molly M. Huntsman, Serapio M. Baca, E Mae Guthman, and Matthew N. Svalina
- Subjects
congenital, hereditary, and neonatal diseases and abnormalities ,Biology ,Neurotransmission ,Inhibitory postsynaptic potential ,Amygdala ,feed-forward inhibition ,Synaptic Transmission ,lateral amygdala ,Fragile X Mental Retardation Protein ,Mice ,Neurodevelopmental disorder ,E/I balance ,medicine ,Animals ,Mice, Knockout ,synaptic plasticity ,General Neuroscience ,General Medicine ,medicine.disease ,FMR1 ,Fragile X syndrome ,Disease Models, Animal ,medicine.anatomical_structure ,Fragile X Syndrome ,Synaptic plasticity ,Synapses ,Disorders of the Nervous System ,Neuroscience ,Research Article: New Research ,Basolateral amygdala - Abstract
Fragile X syndrome (FXS) is a neurodevelopmental disorder (NDD) characterized by intellectual disability, autism spectrum disorders (ASDs), and anxiety disorders. The disruption in the function of the FMR1 gene results in a range of alterations in cellular and synaptic function. Previous studies have identified dynamic alterations in inhibitory neurotransmission in early postnatal development in the amygdala of the mouse model of FXS. However, little is known about how these changes alter microcircuit development and plasticity in the lateral amygdala (LA). Using whole-cell patch clamp electrophysiology, we demonstrate that principal neurons (PNs) in the LA exhibit hyperexcitability with a concomitant increase in the synaptic strength of excitatory synapses in the BLA. Further, reduced feed-forward inhibition appears to enhance synaptic plasticity in the FXS amygdala. These results demonstrate that plasticity is enhanced in the amygdala of the juvenile Fmr1 knock-out (KO) mouse and that E/I imbalance may underpin anxiety disorders commonly seen in FXS and ASDs.Significance StatementThese studies identify significant cellular and synaptic defects in a behaviorally-relevant brain to the pathology of fragile X syndrome (FXS). We find that principal neurons (PNs) in the FXS basolateral amygdala (BLA) exhibit marked hyperexcitability as early as P21. Further, we show that feed-forward inhibition is reduced in the Fmr1 knock-out (KO) LA. This contributes to enhanced synaptic plasticity in LA of the Fmr1KO mouse.
- Published
- 2021
47. No detectable effect on visual responses using functional mri in a rodent model of a-synuclein expression
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Freja Gam Østergaard, Christian Stald Skoven, Hartwig R. Siebner, Tim B. Dyrby, Alex R. Wade, Bettina Laursen, and Kenneth Vielsted Christensen
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vision ,DISORDERS ,Vision ,Substantia nigra ,Rodentia ,WAXHOLM SPACE ATLAS ,Striatum ,superior colliculus ,chemistry.chemical_compound ,α-synuclein ,SDG 3 - Good Health and Well-being ,HUMAN ALPHA-SYNUCLEIN ,MAGNETIC-RESONANCE ,alpha-Synuclein/genetics ,Medicine ,Animals ,rat ,Superior colliculus ,Dependovirus/genetics ,Alpha-synuclein ,ANESTHESIA ,Blood-oxygen-level dependent ,medicine.diagnostic_test ,Pars compacta ,business.industry ,General Neuroscience ,fMRI ,Neurodegenerative Diseases ,A-synuclein ,General Medicine ,Dependovirus ,Magnetic Resonance Imaging ,Rats ,nervous system diseases ,Disease Models, Animal ,chemistry ,nervous system ,FMRI ,Research Article: Negative Results ,alpha-Synuclein ,Biomarker (medicine) ,Rat ,Disorders of the Nervous System ,business ,Functional magnetic resonance imaging ,Neuroscience - Abstract
Parkinson’s disease (PD) is a progressive neurodegenerative disease that is typically diagnosed late in its progression. There is a need for biomarkers suitable for monitoring the disease progression at earlier stages to guide the development of novel neuroprotective therapies. One potential biomarker, α-synuclein, has been found in both the familial cases of PD, as well as the sporadic cases and is considered a key feature of PD. α-synuclein is naturally present in the retina, and it has been suggested that early symptoms of the visual system may be used as a biomarker for PD. Here, we use a viral vector to induce a unilateral expression of human wildtype α-synuclein in rats as a mechanistic model of protein aggregation in PD. We employed functional magnetic resonance imaging (fMRI) to investigate whether adeno-associated virus (AAV) mediated expression of human wildtype α-synuclein alter functional activity in the visual system. 16 rats were injected with either AAV-α-synuclein (n=7) or AAV-null (n=9) in the substantia nigra pars compacta of the left hemisphere. The expression of α-synuclein was validated by a motor assay and post-mortem immunohistochemistry. Five months after the introduction of the AAV-vector, fMRI showed robust blood oxygen level dependent (BOLD) responses to light stimulation in the visual systems of both control and AAV-α-synuclein animals. However, our results demonstrate that the expression of AAV-α-synuclein does not affect functional activation of the visual system. This negative finding suggests that fMRI-based read-outs of visual responses may not be a sensitive biomarker for PD. Significance statement We injected an adeno-associated virus (AAV) vector in rats to induce unilateral expression of human wildtype α-synuclein in the substantia nigra, and in the ipsilateral striatum and superior colliculus (SC). This did not affect functional activation of SC as probed with functional MRI. This negative finding discourages the use of functional brain mapping of visually evoked activity as an indicator of regional expression of human α-synuclein.
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- 2021
48. SCD Inhibition Protects from α-Synuclein-Induced Neurotoxicity But Is Toxic to Early Neuron Cultures
- Author
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Melissa Bennion, Justin W. Nicholatos, Andreas Weihofen, Joost Groot, Thomas M. Carlile, Isin Dalkilic-Liddle, Lori Hrdlicka, David T. Tran, Shekhar Dhokai, and Warren D. Hirst
- Subjects
congenital, hereditary, and neonatal diseases and abnormalities ,Parkinson's disease ,cell-based assays ,Cell ,lipids ,Neuroblastoma ,synuclein ,hemic and lymphatic diseases ,neurotoxicity ,medicine ,Animals ,Humans ,Neurons ,Gene knockdown ,business.industry ,General Neuroscience ,Neurodegeneration ,Neurotoxicity ,Parkinson Disease ,General Medicine ,medicine.disease ,Rats ,SCD ,medicine.anatomical_structure ,Toxicity ,Cancer research ,Synuclein ,alpha-Synuclein ,Parkinson’s disease ,Disorders of the Nervous System ,Neuron ,business ,Stearoyl-CoA Desaturase ,Research Article: New Research - Abstract
Here we report the independent discovery and validation of Stearoyl-CoA desaturase (SCD) as a modulator of alpha-synuclein (αSyn) induced pathology and toxicity in cell-based Parkinson’s Disease models (PD). We identified SCD as top altered gene from transcriptional profiling in primary neurons exogenously expressing αSyn with the amplified familial PD mutation 3K. Thus, we sought to further explore SCD as a therapeutic target in neurodegeneration. We report that SCD inhibitors are toxic to early human and rat neuron cultures while displaying minimal toxicity to late cultures. The fatty acid product of SCD, oleic acid, fully rescues this toxicity in early cultures, suggesting on-target toxicity. Furthermore, SCD inhibition rescues αSyn 3K induced toxicity in late primary neurons. We also confirm that SCD inhibitors reduce formation of αSyn accumulations while oleic acid increases these accumulations in an αSyn 3K neuroblastoma model. However, we identify a caveat with this model where αSyn 3K levels can be suppressed by high SCD inhibitor concentrations, obscuring true effect size. Further, we show that both SCD1 or SCD5 knockdown reduce αSyn 3K accumulations and toxicity, making both a putative drug target. Overall, we confirm key findings of published data on SCD inhibition and its benefits in αSyn accumulation and stress models. The differential neurotoxicity induced by SCD inhibition based on neuron culture age must be accounted for when researching SCD in neuron models and has potential clinical implications. Lastly, our gene profiling studies also revealed novel putative genes connected to αSyn neurotoxicity that are worth further study. Significance Statement There is no disease-modifying therapeutic for those suffering from Parkinson’s Disease (PD). Recent research has shown stearoyl-CoA desaturase (SCD) inhibition to ameliorate alpha-synuclein (αSyn) related pathology and neurotoxicity in pre-clinical PD models. The use of neuronal cell models to study PD related pathology is critical for developing putative therapeutics. In this work we demonstrate important caveats in cellular PD models when studying SCD inhibition. We also independently identified SCD and other genes as potential targets for PD. Overall, this work supports SCD as a clinical target and adds important considerations for studying SCD in in vitro models.
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- 2021
49. Characterization of Seizure Induction Methods in
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Jurga, Mituzaite, Rasmus, Petersen, Adam, Claridge-Chang, and Richard A, Baines
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Epilepsy ,model ,fungi ,seizure induction ,antiepileptic ,Drosophila melanogaster ,Seizures ,Animals ,Drosophila Proteins ,Humans ,Anticonvulsants ,Drosophila ,Disorders of the Nervous System ,insect ,Research Article: New Research - Abstract
Epilepsy is one of the most common neurologic disorders. Around one third of patients do not respond to current medications. This lack of treatment indicates a need for better understanding of the underlying mechanisms and, importantly, the identification of novel targets for drug manipulation. The fruit fly Drosophila melanogaster has a fast reproduction time, powerful genetics, and facilitates large sample sizes, making it a strong model of seizure mechanisms. To better understand behavioral and physiological phenotypes across major fly seizure genotypes we systematically measured seizure severity and secondary behavioral phenotypes at both the larval and adult stage. Comparison of several seizure-induction methods; specifically electrical, mechanical and heat induction, show that larval electroshock is the most effective at inducing seizures across a wide range of seizure-prone mutants tested. Locomotion in adults and larvae was found to be non-predictive of seizure susceptibility. Recording activity in identified larval motor neurons revealed variations in action potential (AP) patterns, across different genotypes, but these patterns did not correlate with seizure susceptibility. To conclude, while there is wide variation in mechanical induction, heat induction, and secondary phenotypes, electroshock is the most consistent method of seizure induction across known major seizure genotypes in Drosophila.
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- 2021
50. Novel Influences of Sex and APOE Genotype on Spinal Plasticity and Recovery of Function after Spinal Cord Injury
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Rachel S.J. Maggard, Lydia E. Strattan, Chris M. Calulot, Lance A. Johnson, Warren J. Alilain, Daimen R. S. Britsch, and Erin L. Abner
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Apolipoprotein E ,Male ,breathing ,Genotype ,Population ,Disease ,Mice ,Apolipoproteins E ,Sex Factors ,Neuroplasticity ,Medicine ,Animals ,genetics ,education ,Spinal cord injury ,Spinal Cord Injuries ,apolipoprotein E ,education.field_of_study ,Neuronal Plasticity ,business.industry ,General Neuroscience ,Intermittent hypoxia ,General Medicine ,Recovery of Function ,medicine.disease ,Spinal cord ,spinal cord injury ,medicine.anatomical_structure ,Spinal Cord ,plasticity ,Synaptic plasticity ,Disorders of the Nervous System ,Female ,business ,Neuroscience ,Research Article: New Research - Abstract
Spinal cord injuries can abolish both motor and sensory function throughout the body. Spontaneous recovery after injury is limited and can vary substantially between individuals. Despite an abundance of therapeutic approaches that have shown promise in preclinical models, there is currently a lack of effective treatment strategies that have been translated to restore function after SCI in the human population. We hypothesized that sex and genetic background of injured individuals could impact how they respond to treatment strategies, presenting a barrier to translating therapies that are not tailored to the individual. One gene of particular interest is APOE, which has been extensively studied in the brain due to its allele-specific influences on synaptic plasticity, metabolism, inflammation, and neurodegeneration. Despite its prominence as a therapeutic target in brain injury and disease, little is known about how it influences neural plasticity and repair processes in the spinal cord. Utilizing humanized mice, we examined how the e3 and e4 alleles of APOE influence the efficacy of therapeutic intermittent hypoxia (IH) in inducing spinally-mediated plasticity after cervical SCI. IH is sufficient to enhance plasticity and restore motor function after experimental SCI in genetically similar rodent populations, but its effect in human subjects is more variable (Golder, 2005; Hayes et al., 2014). Our results demonstrate that both sex and APOE genotype determine the extent of respiratory motor plasticity that is elicited by IH, highlighting the importance of considering these clinically relevant variables when translating therapeutic approaches for the SCI community. Significance Statement There is currently a critical need for therapeutics that restore motor and sensory function effectively after cervical spinal cord injury. Although many therapeutic approaches, including intermittent hypoxia, are being investigated for their potential to enhance spinal plasticity and improve motor outcomes after SCI, it is unknown whether the efficacy of these treatment strategies is influenced by individuals’ genetic background. Here we show that APOE genotype and sex both play a role in determining the propensity for motor plasticity in humanized mice after cervical SCI. These results indicate that sex and genetic background dictate how individuals respond to therapeutic approaches, thereby emphasizing the importance of developing personalized medicine for the diverse SCI population.
- Published
- 2021
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