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This paper introduces a novel dual-air-gap surface permanent magnet synchronous motor (DAG-SPMSM) and investigates its electromagnetic characteristics. The motor comprises two stators (inner and outer) and a single rotor with permanent magnets on both sides (inner and outer). The magnets are arranged in a zig-zag configuration to isolate the inner and outer stator and rotor magnets. Additionally, a magnetic shield is incorporated into the rotor to further isolate the rotor magnetic flux, which results in a localized short flux path that can reduce core loss. The insertion of the magnetic shield also affects the flux path, reducing torque ripple. The electromagnetic characteristics, such as flux path, flux density, and torque, are analyzed using finite element analysis. The effectiveness of using a magnetic screen is also evaluated, and simple experiments are conducted to validate the observed characteristics of the proposed motor.

Ischemia-induced hypoxia is a common complication associated with numerous diseases and is the most important prognostic factor in retinal vein occlusions (RVOs). Early detection and long-term visualization of retinal tissue hypoxia is essential to understand the pathophysiology and treatment of ischemic retinopathies. However, no effective solution exists to evaluate extravascular retinal tissue oxygen tension. Here, we demonstrate a lipid-polymer hybrid organic room-temperature phosphorescence (RTP) nanoparticle (NP) platform that optically detects tissue hypoxia in real-time with high signal-to-noise ratio. The fabricated NPs exhibit long-lived bright RTP, high sensitivity toward oxygen quenching, and desirable colloidal and optical stability. When tested as a hypoxia imaging probe in vivo using rabbit RVO and choroidal vascular occlusion (CVO) models via intravitreal and intravenous (IV) injections, respectively, its RTP signal is exclusively turned on where tissue hypoxia is present with a signal-to-noise ratio of 12.5. The RTP NP platform is compatible with multimodal imaging. No ocular or systemic complications are observed with either administration route. The developed organic RTP NPs present a novel platform approach that allows for biocompatible, nondestructive detection of tissue hypoxia and holds promise as a sensitive imaging tool to monitor longitudinal tissue oxygen levels and evaluate various hypoxia-driven vascular diseases.

The visual response is one of the most intuitive principles of sensors. Therefore, emission and change of the colors are widely studied for development of chemical, thermal and mechanical sensors. And it is still a challenging issue to fabricate them with a simple working mechanism, high sensitivity, good reliability, and a cost-effective fabrication process. In this study, we propose a mechanical strain sensor, which has 2D photonic crystal structures in nanoscale on stretchable polydimethylsiloxane (PDMS) substrate. Due to the periodic nanostructures, the surface of the sensor produces structural colors. And when it is stretched, the periodicity of the nanostructures changes, which results in the shift of the colors. Multiple nanostructures with different periodicities are integrated on the sensor in order to extend the working range up to 150% with high sensitivity. In addition, reusable and robust molds, which are fabricated by self-assembly of nanoparticles, are used for multiple replications of sensor substrates. Thus, the fabrication process of this study is believed to be potential for possible industrial manufacturing. This study is expected to contribute to strain sensors in the future for the applications of health care, infrastructure monitoring, soft robotics, and wearable devices.

The necessity of a simple measurement of platelet activation has been increasing in clinical medicine to regulate the proper dose of the antiplatelet drugs for patients having clinical outcomes in acute situations such as angina pectoris, stroke, or peripheral vascular disease or procedures involving angioplasty or coronary thrombolysis. We developed a self-signaling polydiacetylene (PDA) liposome microarray to detect activated platelets from whole blood samples in a single step. A specific antibody, 9F9 antibody, to platelet-bound fibrinogen was selected and conjugated to the PDA liposome microarray to quantify the fibrinogen-bound platelets. The developed PDA liposome-9F9 microarray generated an intense fluorescence signal when activated platelets in whole blood were introduced and also successfully distinguished the reduced platelet activation in the presence of Tirofiban, a model antiplatelet drug. The results of this single-step benchtop assay incorporates simple, sensitive, and rapid attributes that can detect the extent of platelet activation prior to needed clinical procedures.

In this study, the operation characteristics of an interior permanent magnet synchronous motor (IPMSM) for an electric oil pump (EOP) are analyzed, based on the irreversible demagnetization of the permanent magnet due to temperature saturation. EOP motors are targeted to operate at low-speed high-torque and high-speed low-torque under low and high temperatures, respectively. Hence, the operating temperature is essential for determining the performance characteristics of these motors. Moreover, as a NdFeB type permanent magnet (PM) is utilized, the motor is vulnerable to high temperatures in terms of the irreversible demagnetization of the PM. In this study, the output performance and PM demagnetization ratio of an IPMSM is analyzed at a target ambient temperature. Subsequently, the saturation temperature for each operating point is derived using MotorCAD, which is based on a precise analysis of the thermal equivalent circuit. Finally, the IPMSM for an EOP is analyzed using the derived saturation temperature and compared to the that derived using the ambient temperature.

This paper proposed the transformer characteristic analysis method for the wireless power transfer (WPT) device and linear motor (LM) integration system that can be applied to industrial cleanroom transfer systems. A cable is required to supply the power in conventional systems. In comparison, the proposed system utilizes a WPT device that can simplify power transfers and make a better space utilization. The shape of the wireless power transmission system is proposed along with the discussion of the 2D FEA analysis method about the inductance analyzing method, which are important parameters in magnetic coupling. In addition, ferrite iron loss was calculated based on the analysis results, and applied to the entire modeling circuit to verify the validity of the measured and analyzed values. Finally, the proposed analysis method for the transformer coupling characteristics of the wireless power transfer combined with the transfer system is verified by experiments and simulations.

In this study, we report a method to fabricate molds and flexible stamps with 2D photonic crystal structures. This includes self-assembly of polystyrene particles into monolayer, oxygen reactive ion etching, thin film (chromium (Cr)) deposition, and polydimethylsiloxane replication. By tuning the thickness of Cr layer, reusable master molds with nano bumps or nano concaves could be prepared selectively. We showed that the replicated flexible stamps out of these molds exhibited structural colors. Characteristics of the colors depended on viewing angle, brightness of background and light source. And the colors even faded out when the background is white or when the stamp was bent. By using this feature, possible strategies for anti-counterfeiting applications have been suggested in this study. Since the molds are reusable and the fabrication method is simple and cost-effective, this study is expected to contribute to nano devices for industries in future.

Retinal vein occlusion (RVO) is a common cause of vision impairment and blindness. In RVO, thrombosis in a retinal vein and reduction in blood flow leads to the development of retinal hypoxia. Retinal hypoxia stimulates vascular endothelial cell growth factor (VEGF) production, resulting in the formation of neovascularization and vision loss. Currently, non-invasive imaging modalities are not available to track and quantify the tissue-level hypoxia in living animals. In this study, a novel organic room-temperature phosphorescence nanoparticle (PNPs) was developed and characterized for identification of retinal hypoxia in real-time in living rabbits. The ability of PNPs for hypoxia imaging was examined in rabbits with laser-induced RVO models (n=3). The location of retinal hypoxia was imaged before and after the intravitreal administration of PNPs at a concentration of 2.5 mg/mL and an injection dose of 50 µL. Phosphorescence imaging was acquired at different time points over 7 days alo

Permanent magnet Vernier machine (PMVM) is a strong candidate for direct-drive applications in low-speed region because its power characteristic is higher than conventional permanent magnet machine (PMM). In this paper, the design of a dual-stator PMVM (DSPMVM) with spoke-type rotor is introduced. As a radial motor with dual-stator configuration, one on the outer and inner side, the rotor is equipped with support bars for practical and simple manufacturing, which is the aim of this study. The characteristics and performance of the proposed machine with and without the support bar are examined with finite element analysis (FEA). The DSPMVM and the support were manufactured and tested through experiments to verify proposed structure. Both simulation and experiment results show that there is little to no difference in performance when the support bar is equipped. Furthermore, the average deviation between simulation and experiment results is approximately 7% which is within the acceptable range.

A versatile organic room-temperature phosphorescence (RTP)-based "turn on" biosensor platform has been devised with high sensitivity by combining oxygen-sensitive lipid-polymer hybrid RTP nanoparticles with a signal-amplifying enzymatic oxygen scavenging reaction in aqueous solutions. When integrated with a sandwich-DNA hybridization assay on 96-well plates, our phosphorimetric sensor demonstrates sequence-specific detection of a cell-free cancer biomarker, a TP53 gene fragment, with a sub-picomolar (0.5 p.m.) detection limit. This assay is compatible with detecting cell-free nucleic acids in human urine samples. Simply by re-programming the detection probe, our unique methodology can be adapted to a broad range of biosensor applications for biomarkers of great clinical importance but difficult to detect due to their low abundance in vivo.

Direct fabrication of a three-dimensional (3D) structure using soft materials has been challenging. The hybrid bilayer is a promising approach to address this challenge because of its programable shape-transformation ability when responding to various stimuli. The goals of this study are to experimentally and theoretically establish a rational design principle of a hydrogel/elastomer bilayer system and further optimize the programed 3D structures that can serve as substrates for multi-electrode arrays. The hydrogel/elastomer bilayer consists of a hygroscopic polyacrylamide (PAAm) layer cofacially laminated with a water-insensitive polydimethylsiloxane (PDMS) layer. The asymmetric volume change in the PAAm hydrogel can bend the bilayer into a curvature. We manipulate the initial monomer concentrations of the pre-gel solutions of PAAm to experimentally and theoretically investigate the effect of intrinsic mechanical properties of the hydrogel on the resulting curvature. By using the obtained results as a design guideline, we demonstrated stimuli-responsive transformation of a PAAm/PDMS flower-shaped bilayer from a flat bilayer film to a curved 3D structure that can serve as a substrate for a wide-field retinal electrode array.

We developed a paper-based analytical device (μPAD) combined with self-signaling polydiacetylene (PDA) liposomes for convenient visual neomycin detection. The simple dot array type of μPAD was fabricated by the wax printing technique, and the PDA liposomes in the aqueous solution were facilely immobilized onto the hydrophilic dot region of the paper substrate. We found that, when the PDA liposomes were inserted to the paper matrix, the stability of the PDA liposomes can be significantly enhanced by adding a hydrophilic reagent such as polyvinyl alcohol and glycerol to the liposome solution. In particular, polyvinyl alcohol (PVA) provides the best stabilization among the various hydrophilic reagents tested in this contribution, and the enhanced stability sharply increased the sensitivity of the PDA liposomes in the paper matrix. Based on the above results, we successfully detected neomycin through both naked-eye observation and fluorescence measurement of PDA signals. The detection limit was 1 ppm and was selective to non-aminoglycoside antibiotics.

Oxidative stress plays a critical role in cardiac injury during ischemia/reperfusion (I/R). Despite a potent cardioprotective activity of KR-33889, a novel poly (ADP-ribose) polymerase inhibitor, its underlying mechanism remains unresolved. This study was designed to investigate the protective effects of KR-33889 against oxidative stress-induced apoptosis in rat cardiomyocytes H9c2 cells and isolated rat hearts. H2O2 caused severe injury to H9c2 cells, mainly due to apoptosis, as revealed by TUNEL assay. However, KR-33889 pretreatment significantly attenuated H2O2-induced apoptosis of H9c2 cells, which was accompanied by decrease in expression of both cleaved caspase-3 and Bax and increase in Bcl-2 expression and the ratio of Bcl-2/Bax. KR-33889 also significantly enhanced the expression of anti-oxidant enzymes including heme oxygenase-1, Cu/Zn-superoxide dismutase (SOD), Mn-SOD, and catalase, thereby inhibiting production of intracellular ROS. Furthermore, KR-33889 reversed H2O2-induced decrease in phosphorylation of Akt, GSK-3β, ERK1/2, p38 MAPK, and SAPK/JNK during most H2O2 exposure time. In globally ischemic rat hearts, KR-33889 inhibited both I/R-induced decrease in cardiac contractility and apoptosis by increasing Bcl-2, decreasing both cleaved caspase-3 and Bax expression, and enhancing expression of anti-oxidant enzymes. Taken together, these results suggest that KR-33889 may have therapeutic potential to prevent I/R-induced heart injury in ischemic heart diseases mainly by reducing oxidative stress-mediated myocardial apoptosis.

In this paper, finite element analysis demonstrates the difference between dual-permanent-magnet-excited machines (DPMM) and surface-mounted permanent magnet machines (SPM) in terms of tangential force at the same air gap, diameter, stacking length, and input current. Different from most conventional machines, a novel DPMM has two sets of permanent magnets employed on both stator and rotor. To make a fair comparison, the novel DPMM, based on an original design, is specified to have the same dimensions as a conventional SPM. With the aid of 2D finite element analysis, tangential force generated from the novel DPMM is 167.65% higher than the conventional SPM. To verify the validity of the analyses, a prototype was fabricated and tested. Experiments showed that average deviation was only approximately 1.85%.

The actual trend in high speed electromechanical drives technology is to use permanent magnet (PM) brushless motors, solid rotor induction motors or switched reluctance motors. The highest efficiency and highest power density is achieved with PM brushless motors. The following issues, which are essential in electromagnetic, mechanical and thermal design of high speed PM brushless motors have been discussed: 1) main dimensions and sizing procedure, 2) mechanical requirements, 3) stator design guidelines including bad practices, 4) rotor design guidelines.

The stress-responsive bioprocess concept has been developed into an environmentally friendly biosensor with low energy consumption and high recovery yield. To improve chitosanase production during Streptomyces griseus cultivation, heat-stress was applied to the bioprocess with monitoring using an on-line gas sensing system. When exposed to heat-stress with liposomes, the chitosanase productivity was 2.6 times greater than for conventional cultivation. The mixed gas components could be distinguished using a principal component analysis during chitosanase production. The online-monitoring system reflected basic changes in growth conditions and metabolite formation in cells. The array type gas-sensing system was capable of detecting bacterial infection faster than conventional sensor systems. The gas sensor system can play a key role in monitoring and controlling stress levels in a stress-responsive bioprocess.

Alginate-based nanoparticles were generated by mixing two types of stock solutions through a microfluidic device. In our microfluidic mixing device, aqueous Ca-alginate pre-gels and cationic poly-l-lysine (PLL) solutions were mixed, and the polyelectrolyte complexation that subsequently occurred between the pre-gel and PLL resulted in precipitates, yielding alginate nanoparticles. Due to the faster microfluidic mixing achieved compared with that obtained with the conventional bulk mixing method, the alginate nanoparticles exhibited enhanced aggregation stability. The control of the size of the nanoparticles through the tuning of the flow rates in the microfluidic device was also achieved.

Immobilization of bioanalytes (e.g., protein, lipid membrane, and cells) within a microfludic channel is a useful strategy in diverse biological analysis including biosensor, diagnosis, and biochemical reactions. Such microfluidic systems can offer miniaturized platforms with distinct advantages such as reduced use of samples or reagents and increased resolution. In particular, polymeric microfluidic devices (e.g., polydimethylsiloxane (PDMS)) bound to a substrate have been widely used. However the non-specific adsorption of bioanalytes is serious problem for microfluidic biological assays, especially for dilute samples. To overcome such limitations, a simple and widely applicable microfluidic channels combined with polyethylene glycol (PEG) microwells have been developed. In this review, we summarize the methods and application of PEG microwells with a particular emphasis on integrated microfluidic systems. The assembly between PEG microwell and microchannel enables precise delivery and manipulation of the biosamples, leading to development of miniaturized diagnostic assays, microreactors, and multiple screening platforms for tissue engineering and cell biology.

Most solid-state biosensor platforms require a specific immobilization chemistry and a bioconjugation strategy separately to tether sensory molecules to a substrate and attach specific receptors to the sensory unit, respectively. We developed a mussel-inspired universal conjugation method that enables both surface immobilization and bioconjugation at the same time. By incorporating dopamine or catechol moiety into self-signaling polydiacetylene (PDA) liposomes, we demonstrated efficient immobilization of the PDA liposomes to a wide range of substrates, without any substrate modification. Moreover, receptor molecules having a specificity toward a target molecule can also be attached to the immobilized PDA liposome layer without any chemical modification. We applied our mussel-inspired conjugation method to a droplet-array biosensor by exploiting the hydrophilic nature of PDA liposomes coated on a hydrophobic polytetrafluoroethylene surface and demonstrated selective and sensitive detection of vascular endothelial growth factor down to 10 nM.

Dept. of Medical History, College Korean Medicine, KyungHee UniversityObjectives : King Hyojong (1619-1659) was the 17th king of the Joseon Dynasty. He was on the throne from 1649 to 1659. The king was a center of state rule in ancient times. This meant that the physical and mental status of a king was very confidential information. In the royal court of Joseon Korea, the treatment process for the king had to be performed under the surveillance of close associates, and this whole process had to be recorded by the official secretary. This is the reason that I referred to the Daily Records of Royal Secretariat of Joseon Dynasty. Methods : Relevant articles were extracted from Daily Records of Royal Secretariat of Joseon Dynasty and analyzed according to the main arguments of this paper. Some other related sources were referred in terms of King Hyojong’s ailments and treatments.Results & Conclusions : King Hyojong suffered from various diseases during his time on the throne. Fever was the most frequent ailment; he also often had diarrhea followed by poor appetite. I assume that he must have suffered from diabetes considering his ailments. Additionally, his final treatment records clearly show that his ailments were similar to diabetic complications.Key Words : King Hyojong, Korean Medicine, Royal Secretariat of Joseon Dynasty, Joseon Medicine, Medical Records.

Janus-compartmental alginate microbeads having two divided phases of sensory polydiacetylene (PDA) liposomes and magnetic nanoparticles were fabricated for facile sensory applications. The sensory liposomes are composed of PDA for label-free signal generation and 1,2-dipalmitoyl-sn-glycero-3-galloyl (DPGG) lipids whose galloyl headgroup has specific interactions with lead(II). The second phase having magnetic nanoparticles is designed for convenient handling of the microbeads, such as washing, solvent exchange, stirring, and detection, by applying magnetic field. Selective and convenient colorimetric detection of lead(II) and efficient removal of lead(II) by alginate matrix at the same time are demonstrated.

Cordycepin (3'-deoxyadenosine) isolated from Cordyceps militaris, a species of the fungal genus Cordyceps, has been shown to exhibit many pharmacological functions, such as anticancer, anti-inflammatory, and antioxidant activities. In this study, we investigated the preventive role of cordycepin in ischemic/reperfusion (I/R) injury of isolated rat hearts and anesthetized rats. After Sprague-Dawley rats received cordycepin (3, 10, and 30 mg/kg) or control (0.5 % carboxyl methylcellulose) orally once a day for a week, hearts were isolated and mounted on Langendorff heart perfusion system. Isolated hearts were perfused with Krebs-Henseleit buffer for 15-min pre-ischemic stabilization period and subjected to 30-min global ischemia and 30-min reperfusion. Cordycepin administration (10 mg/kg, p.o.) significantly increased left ventricular developed pressure during the reperfusion period compared to that in the control group, but without any effect on coronary flow. Cordycepin (10 mg/kg, p.o.) significantly increased the phosphorylation of Akt/GSK-3β/p70S6K pathways, which are known to modulate multiple survival pathways. In addition, cordycepin decreased Bax and cleaved caspase-3 expression while increasing Bcl-2 expression, Bcl-2/Bax ratio, and heme oxygenase (HO-1) expression in isolated rat hearts. In anesthetized rats subjected to 30 min occlusion of left anterior descending coronary artery/2.5-h reperfusion, cordycepin (1, 3, and 10 mg/kg, i.v.) administered 15 min before the onset of ischemia dose-dependently decreased the infarct size in left ventricle. In conclusion, cordycepin could be an attractive therapeutic candidate with oral activity against I/R-associated heart diseases such as myocardial infarction.

This paper presents a characteristic analysis method of a permanent magnet type transverse flux linear motor (TFLM) with spiral cores. The spiral cores are used as the mover cores in order to make 3-dimensional (3-D) magnetic flux paths at the TFLM which have 3-D magnetic flux flows. The 3-D Equivalent Magnetic Circuit Network Method is used to analyse the magnetic characteristics of the machine, and an imaginary part, ‘flux barrier,’ is introduced to consider the spiral core characteristic. Magnetic parameters such as flux, inductance, and thrust are calculated from the analysis results. The computed thrust forces are compared to measured values to confirm the accuracy of the analysis.

Carbon-supported Pt (Pt/C) catalyst was selectively functionalized with thermally responsive poly(N-isopropylacrylamide) (PNIPAM) to improve water transport in the cathode of proton exchange membrane fuel cell (PEMFC). Amine-terminated PNIPAM selectively reacted with the functional group of −COOH on carbon surfaces of Pt/C via the amide reaction by 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide (EDC) as a catalyst. Pt surfaces of Pt/C were intact throughout the carbon surface functionalization, and the carbon surface property could be thermally changed. The PNIPAM-functionalized Pt/C was well-dispersed, because of its hydrophilic surface property at room temperature during the catalyst ink preparation. In sharp contrast, when PEMFC was operated at 70 °C, PNIPAM-coated carbon surface of Pt/C became hydrophobic, which resulted in a decrease in water flooding in the cathode electrode. Because of the switched wetting property of the carbon surface, PEMFC with PNIPAM-functionalized Pt/C catalyst in the cathode ...

This paper deals with the optimum design focused on an LHS (Latin hypercube sampling) experiment with the many design variables of a developed motor and comparison of the optimum algorithm. The effective design variables are selected by screening and analysis of means (ANOM). In this paper, the optimum design is achieved by using the comparison response surface methodology (RSM) with the genetic algorithm (GA) method as the multiobjective optimum design. The torque ripple ratio is satisfied with a design goal within 3%. Therefore, multiobjective optimum design is considered between average torque and efficiency. The simulation results are compared with the experiment and show a deviation below 5%.

Transverse flux machine (TFM) has been proved to be very suitable for high-torque, lowspeed, and direct-drive situation in industry. But the complex structures and costly permanent magnets (PMs) are two key limitations of its wide range of applications. This paper proposes a new claw pole TFM (ACPTFM) which features an assembled claw pole stator and using the lamination steels material to overcome the complex structures. By combining response surface methodology (RSM) with design of experiment, an optimum design method is put forward to improve the PM’s contribution to the torque in order to save the PM’s amount. The optimum design results demonstrate the validity of the proposed optimum design method and the optimized model. Eventually, the finiteelement analysis (FEA) calculation method, which is used in the optimization process, is verified by the experiments in a prototype.

This paper presents an eddy current loss analysis for a transverse flux rotary machine (TFRM) with laminated stator cores, which consist of inner and outer cores whose laminated directions are perpendicular to each other. Although the TFRM is laminated to reduce eddy current losses, it still exhibits rapidly increasing core losses as the frequency increases. To solve this problem, slits are introduced to the stator outer core. 3-dimensional finite element analysis (3D FEA) based on the T- Ω formulation is used to solve the eddy-current problem for a various numbers of slits in the nonlinear lamination core. The effects of the slits are confirmed using experiment data and 3D FEA results.

Polymers provide a versatile platform for mimicking various aspects of physiological extracellular matrix properties such as chemical composition, rigidity, and topography for use in cell and tissue engineering applications. In this review, we provide a brief overview of patterning methods of various polymers with a particular focus on biocompatibility and processability. The materials highlighted here are widely used polymers including thermally curable polydimethyl siloxane, ultraviolet-curable polyurethane acrylate and polyethylene glycol, thermo-sensitive poly(N-isopropylacrylamide) and thermoplastic and conductive polymers. We also discuss how micro- and nanofabricated polymeric substrates of tunable elastic modulus can be used to engineer cell and tissue structure and function. Such synergistic effect of topography and rigidity of polymers may be able to contribute to constructing more physiologically relevant microenvironment.