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Aims: Zoonotic arboviruses (arthropod‐borne) of the Orthoflavivirus genus, such as West Nile virus (WNV), Usutu virus (USUV) and Tick‐borne encephalitis virus (TBEV), are emerging in Northwestern Europe and pose a threat to both human and animal health. In the Netherlands, passive symptomatic surveillance (notification of clinical cases) in horses is one of the main pillars for the early detection of WNV. For such passive surveillance to work properly, horse owners and veterinarians need to recognize symptoms and report suspected cases to the authorities. Currently, little is known about the seroprevalence of orthoflaviviruses in domestic animals in the Netherlands. Therefore, this study aims at identifying the seroprevalence of WNV and USUV in horses and dogs in the Netherlands. Additionally, this study seeks to evaluate the knowledge and perceptions of Dutch horse owners towards mosquito‐borne viruses. Methods and Results: A cross‐sectional serosurvey in horses and dogs was conducted between May 2021 and May 2022. Serum samples were screened using an ELISA and doubtful and positive samples were confirmed by Virus Neutralization Tests for WNV, USUV and TBEV. A validated questionnaire, the MosquitoWise survey, was used to assess the knowledge and perceptions of Dutch horse owners towards mosquito‐borne viruses between July and October 2022. The serosurvey revealed a low seroprevalence for WNV in horses and no WNV‐positive dogs were found. Similarly, a low USUV seroprevalence was found in dogs. The MosquitoWise survey revealed a high knowledge level for horse owners and high awareness of WNV vaccination but a more limited intent to vaccinate. Conclusions: The low seroprevalences of WNV and USUV indicate many dogs and horses remain susceptible, offering opportunities for trend analysis and surveillance. However, despite multiple recent detections of WNV, USUV, and TBEV in humans, the role of dogs and horses in early detection of human cases is debatable. High awareness among horse owners and the absence of detected equine WNV cases highlight this uncertainty. Continued surveillance is crucial for detecting increased virus circulation and protecting both animal and human health. [ABSTRACT FROM AUTHOR]

The Caspian pond turtle, Mauremys caspica, is an important reptile species in the southern basin of Caspian Sea. Haematological indices and serum biochemistry can be utilised as valuable screening tools for the health status of many domestic and wild animals. In this study, the blood parameters were measured in male and female turtles to establish physiologic reference ranges of selected haematological and serum biochemical parameters in M. caspica. For this purpose, blood samples were collected from 60 Caspian pond turtles (30 males and 30 females) from the southern basin of Caspian Sea. Significant differences were observed in some haematological indices (i.e. haemoglobin and haematocrit) and serum biochemical indices (i.e. alkaline phosphatase, aspartate aminotransferase, and creatine kinase) between the male and female turtles (P<0.05). However, further research is necessary to elucidate the influence of size, sex, season, and habitat variations on haemato-biochemical parameters in the Caspian pond turtle. [ABSTRACT FROM AUTHOR]

Dicrocoelium dendriticum is a trematode that infects both domestic and wild animals, with transmission potentially occurring at the wildlife-domestic interface. This study aims to investigate liver fluke infection in roe deer (Capreolus capreolus) and its epidemiological role in central Italy, where the species coexists with domestic ruminants. A total of 265 roe deer livers were examined between 2021 and 2022 and animal data such as collection area, sex, age class, and seasonality were recorded. The livers were macroscopically examined to determine a Liver Lesion Score, and adult parasites were collected and counted. Overall, 52 of the 265 animals (19.6%) were infected by D. dendriticum flukes, identified through morphological and molecular methods. The total mean intensity of infection was 17.1 flukes per animal (ranging from 1 to 354). This study reports, for the first time, the presence of D. dendriticum in roe deer in Italy. The lower intensity of infection observed in roe deer compared to domestic animals in the literature may be related to the different dietary habits of these species. However, further studies are recommended to access (i) the potential role of roe deer as an animal in "refugia" and (ii) whether interactions between wild and domestic animals contribute to anthelmintic resistance in the latter. [ABSTRACT FROM AUTHOR]

Acari is a diverse group of arthropods that include well-known parasites of animals. Rodents, particularly, serve as common hosts of mites and ticks, transmitting pathogens to domestic animals and humans. Understanding the ecological dynamics between parasites and rodent hosts is crucial for ecosystem management. Due to limited knowledge about the life history of ectoparasites in wild mouse nests, we collected four nests of Apodemus speciosus, the most common rodent species in the wild areas of Japan, along with soil samples near the nests to study arthropod communities. Mites overwhelmingly populated the mouse nests, comprising approximately 90% of all arthropods, while both mites and collembolans were prevalent in soil. Various species identified in our study, such as those from the families Laelapidae, Pygmephoridae, Cheyletidae, Trombiculidae, Glycyphagidae, and Thyrisomidae align with known ectoparasites or species found in the nests of other rodent species, but most parasitic species were never collected in the surrounding soil except for trombiculids. The dominance of mites in mouse nests suggests selective preference for inhabiting these host environments, although the exact reasons driving this dominance remain unclear. Further investigations into the food web within mouse nests will aid in characterising faunal composition and understanding the ecological interaction among rodents, mites, and other nest symbionts. [ABSTRACT FROM AUTHOR]

Although vocal communication between humans and domestic animals is common, there has been limited research on how humans perceive the emotional content of animal vocalizations. This study investigated the relationship between pet ownership and sensitivity to negative emotion in dog and cat distress vocalizations (n = 237; 57% pet owners). Empathy toward animals and empathy toward humans were explored as possible mediators. The links between pet owners’ levels of sensitivity to animal distress vocalizations, empathy toward animals, and pet attachment and the care they provided to their pets were also examined. The findings revealed that current pet owners perceived both dog and cat distress vocalizations to be sadder than current non-pet owners. The link between pet ownership and sensitivity to dog (but not cat) distress vocalizations was fully mediated by empathy toward animals, but not mediated by empathy toward humans. Sensitivity to negative emotion in distress vocalizations was not a strong indicator of the level of care provided to dogs and cats. On the other hand, higher levels of empathy toward animals and pet attachment might be better indicators of humans providing more stimulating and enriched lives for their pets. [ABSTRACT FROM AUTHOR]

Empty but suitable habitat exists for many of the world's terrestrial large carnivores, yet reintroductions are often considered difficult. In the Russian Far East, orphaned Amur tiger (Panthera tigris altaica) cubs were brought into captivity but prepared for re‐release into the wild. We addressed 2 questions after reintroduction: 1) were individuals raised in captivity capable of killing prey at a rate sufficient to survive, and 2) did individuals avoid use of domestic animals as a primary source of food? We collected data on hunting behavior of 6 orphaned tigers re‐released into their indigenous range, and compared kill composition, kill rate, and consumption rate to individuals studied within the existing range (Sikhote‐Alin) of Amur tigers. Prey composition of rehabilitated tigers varied from that of the Sikhote‐Alin tigers, but composition of major food groups was nearly identical. Kill rate of rehabilitated tigers was higher and prey size was smaller than that of Sikhote‐Alin tigers, but consumption rates were nearly identical. One young male tiger depredated domestic animals, but other individuals only rarely preyed on dogs or cattle they encountered in forests. We documented high survival, reproduction, and recruitment of re‐released individuals. These results indicate that tigers held in captivity during the majority of their early lives can survive in the wild, so long as exposure to humans is kept to a minimum and individuals learn to hunt wild prey before release. Results provide a potential framework for reintroductions of tigers and other large felids across the globe. [ABSTRACT FROM AUTHOR]

Wildlife that use anthropogenic resources often come into conflict with humans, e.g. due to damaged property, habituation or transmission of pathogens, amongst them Sarcoptes scabiei, the aetiological agent of sarcoptic mange, an emerging panzootic skin disease. This study examines the use of haystacks intended for supplementary feeding of European bison (Bison bonasus) by wolves (Canis lupus) with sarcoptic mange and the potential role of this behaviour in skin parasite transmission and human-wolf conflict. Hay samples from the beds used by wolves were found to harbour S. scabiei mites, even several days after the last use. Our data demonstrate an unforeseen link between wild ungulate supplementary feeding and wolf behaviour that may lead to conflict, namely approaching human settlements. However, no negative interactions were observed between wolves and humans or domestic animals. The presence of S. scabiei mites in haystacks provides a potential for its human-facilitated environmental transmission among wildlife and to domestic animals. [ABSTRACT FROM AUTHOR]

The article presents evidence of antibodies against Crimean-Congo haemorrhagic fever virus in various livestock species in Nigeria. The study screened 467 serum samples from cattle, goats, sheep, and camels collected between 2019-2020 in four Nigerian states. The results showed an overall seroprevalence of 37.7%, with the highest prevalence observed in camels from Kano State. The findings suggest that domestic animals in Nigeria may play a role in the epidemiology of the disease, highlighting the potential risk of human infection. [Extracted from the article]

Attacks by wild and domestic animals represent a significant public health and social issue with implications for both mortality and morbidity. These incidents contribute to many deaths and hospitalizations each year, with statistical variations depending on the native fauna and cultural practices unique to different countries. The ongoing expansion of urban areas, with the reduction of natural habitats and the increasing frequency of human recreational activities in wild environments, is exacerbating human-wildlife interactions, increasing of such incidents.In forensic pathology, when encountering a body with lesions of animal origin, it is crucial first to exclude any potential homicidal dynamics. Following this, it is equally important to accurately differentiate between antemortem injuries and post-mortem scavenging to ensure a correct interpretation of the cause and manner of death. In this paper, three autopsy cases involving animal attacks are described and analysed in detail. Of particular significance is the final case, where the injuries were attributed to predation by red foxes (Vulpes vulpes). Such cases are rare and thus represent an underexplored area within forensic literature, making this case especially noteworthy for the scientific community. Discussing these cases provides valuable insights that can enhance diagnostic approaches in similar forensic scenarios. [ABSTRACT FROM AUTHOR]

The article "Whole genome sequencing and de novo genome assembly of the Kazakh native horse Zhabe" published in Frontiers in Genetics discusses the genetic characteristics of the Kazakh horse breed, specifically the Zhabe type. The study presents high-quality genome assemblies for six Kazakh horses of the Zhabe type, highlighting the importance of genetic research and conservation of genetic diversity in economically important animal varieties. The research utilized Oxford Nanopore Technology for sequencing and assembly, providing valuable insights into the genetic makeup of the Kazakh horse breed. The findings suggest that the Kazakh horse breed has a mixed origin in the maternal lineage, indicating high levels of genetic variability and serving as an important reservoir of genetic biodiversity. [Extracted from the article]

Culicoides are vectors that can transmit many different pathogens to mammals — including humans, and domestic and wild animals — and birds. In order to take preventive measures against any vector-borne disease, it is important to gather information on both the host and vector species. Culicoides species are mainly mammalophilic, ornithophilic or ornithophilic/mammalophilic, but females have also been found to occasionally feed on engorged insects. A recent systematic review based on three groups of key words investigated Culicoides on farms, and asserted that 92 species (including four not present species) have been reported among cattle in mainland France and Corsica. We have re-evaluated the presence of Culicoides species in cattle in France using the same data of the review. Our data show that only 18 species are reported among cattle. Furthermore, our research used molecular and indirect investigations to analyse Culicoides species that had been feeding on cattle. Our results demonstrate that 45 species feed on cattle out of 92 species present in France. The paper discusses the relevance of data in the literature when investigating hosts of Culicoides species. [ABSTRACT FROM AUTHOR]

Background: Vector-borne pathogens (VBPs) are increasing in significance in veterinary medicine and public health settings, with wildlife playing a potentially crucial role in their transmission. Eurasian badgers (Meles meles) are widely distributed across Europe. However, information currently available on the prevalence of VBPs in badgers is limited. The objective of the current study was to investigate the occurrence of Anaplasmataceae, Bartonella spp., Mycoplasma spp., Rickettsia spp., Piroplasmida, Trypanosomatida and Filarioidea in badgers and subsequently, based on the results, assess the potential risk to domestic animals, other wildlife and humans. Methods: Between 2017 and 2021, blood or spleen samples from 220 badgers were collected in nine continental European countries: Austria (n = 7), Bosnia and Herzegovina (n = 2), Croatia (n = 22), France (n = 44), Germany (n = 16), Hungary (n = 7), Italy (n = 16), Romania (n = 80) and Serbia (n = 26). VBPs were identified by performing PCR analysis on the samples, followed by Sanger sequencing. Additionally, to distinguish between different Babesia lineages we performed restriction fragment length polymorphism (RFLP) analysis on piroplasm-positive samples, using HinfI as restriction enzyme. A phylogenetic analysis was performed on Mycoplasma spp. Results: The pathogens identified were Babesia sp. badger type A (54%), B (23%), and C (37%); Trypanosoma pestanai (56%); Mycoplasma sp. (34%); Candidatus Mycoplasma haematomelis (8%); Candidatus Mycoplasma haematominutum (0.5%); and Ehrlichia spp. (2%). Rickettsia spp., Bartonella spp. and filarioid nematodes were not detected among the tested samples. Conclusions: The large sample size and diverse study populations in this study provide valuable insights into the distribution and epidemiology of the analyzed pathogens. Some of the VBPs identified in our study show high similarity to those found in domestic animals, such as dogs. This finding suggests that badgers, as potential reservoirs for these pathogens, may pose a threat not only to other wildlife but also to domestic animals in close vicinity. Continuous surveillance is essential to monitor VBPs in wildlife as a means to enable the assessment of their impact on other wildlife species, domestic animals and human health. [ABSTRACT FROM AUTHOR]

OBJECTIVE To provide comprehensive epidemiological information about the distribution and occurrence of rabies during 2022 in the US, Canada, and Mexico. METHODS The US National Rabies Surveillance System collected 2022 animal rabies data from US state and territorial public health departments and USDA Wildlife Services. Temporal and geographic analyses were conducted to evaluate trends in animal rabies cases. RESULTS During 2022, 54 US jurisdictions reported 3,579 animal rabies cases, reflecting a 2.3% decline from 3,663 cases reported in 2021. Six states collectively reported > 50% of animal rabies cases: Texas (395 [11.0%]), Virginia (337 [9.4%]), Pennsylvania (329 [9.2%]), New York (267 [7.5%]), North Carolina (264 [7.4%]), and California (241 [6.7%]). Out of the total reported rabies animal cases, 3,234 (90.4%) were attributed to wildlife, with bats (1,218 [34.0%]), raccoons (1,014 [28.3%]), skunks (660 [18.4%]), and foxes (269 [7.5%]) representing the primary hosts confirmed with rabies. Rabid cats (222 [6.2%]), cattle (42 [1.2%]), and dogs (50 [1.4%]) constituted > 90% of reported domestic animal rabies cases. CONCLUSIONS In 2022, there was an increase in the number of animal samples submitted for rabies testing in the US and Canada. A notable geographic expansion of gray fox rabies virus variant was detected in the US. Three human rabies deaths due to vampire bat rabies infection occurred in Mexico; none were reported from the US and Canada. CLINICAL RELEVANCE Laboratory diagnosis of rabies in animals is critical to ensure judicious use of human rabies postexposure prophylaxis. [ABSTRACT FROM AUTHOR]

The study experimentally tested an intervention that debunks epistemically suspect beliefs about vaccines. After answering questions about pre‐existing epistemically suspect beliefs (irrational health beliefs and conspiracy mentality), 565 participants were randomly assigned into one of three conditions and exposed either to neutral information about domestic animals, salient epistemically suspect content about vaccination or an intervention that debunks epistemically suspect beliefs about vaccination. Afterwards, the participants answered questions about vaccination‐related conspiracy narratives (manipulation check), vaccination attitudes, intentions to vaccinate against HPV, support for an HPV vaccination programme and intentions to seek health guidance. Although the intervention demonstrated the potential to inhibit the endorsement of conspiracy narratives, we found no differences in the other outcome variables. Nevertheless, across the conditions, pre‐existing epistemically suspect beliefs were associated with less favourable attitudes towards vaccination, lower intentions to vaccinate against HPV, less support for the vaccination programme and lower intentions to seek health guidance. The results indicate that debunking may be futile in curbing long‐term negative impacts associated with epistemically suspect beliefs, and they contribute to the debate about assessing the effectiveness of interventions related to highly controversial topics such as vaccination. The study enhances understanding of persistent adverse impacts that epistemically suspect beliefs may have on public health outcomes. [ABSTRACT FROM AUTHOR]

This study examines farmers' perceptions of agrochemical use in maize production and their harmful environmental effects. A convergent parallel mixed design was used to collect quantitative data from 254 respondents via structured interviews and qualitative data through in-depth interviews and Focus Group Discussions. The findings revealed that among those who were using agrochemicals, 98.9%, 59.2% and 49.7% used inorganic fertilizer to enhance soil fertility, pesticides to control pests and herbicides to kill weeds, respectively. Those who did not use agrochemicals were limited by many factors, including adverse effects of agrochemicals on the environment and lack of access to and the high cost of agrochemicals. Also, more than half of the respondents (51.2%) had limited knowledge about the harmful effects of agrochemicals on the environment. The perceived harmful effects of agrochemicals on the environment included being harmful to domestic animals and non-targeted insects and causing air pollution. The study provides insights for the government on how it could design and enforce educational programmes that would increase farmers' knowledge of the proper use of agrochemicals to protect the environment while maximizing maize production. This study contributes to the existing literature on how unawareness of farmers on the use of agrochemicals can adversely affect the environment. [ABSTRACT FROM AUTHOR]

The interspecies interactions between farmers and livestock have provided an important conceptual and empirical focus in expanding our understanding of animal geographies, with a burgeoning literature exploring the intricate material and discursive (re)positioning of animals across different socio-spatial contexts. This paper offers a new direction for understanding these human-animal encounters by exploring the ways in which farmer and animal identities are mediated by social media. By drawing on a qualitative analysis of UK farmers' tweets about their livestock and semi-structured interviews about farmers' use of social media, this paper examines how social media allows a reworking of the spatiotemporalities of animal-human relations and offers farmers the opportunity to co-construct their identities with their livestock and farming practices. The paper identifies three ways in which farmer–livestock relations are mediated by social media. Firstly, that animals are made visible as actors within farming cultures to those beyond the farm gate. Secondly, that the role of farm animals as subjects and objects is negotiated throughout temporal moments online and thirdly that representations on social media of interactions with animals plays a role in the (re)construction of farmers' identities. [ABSTRACT FROM AUTHOR]

Background: Lyme disease is a tick‐borne zoonotic disease caused by Borrelia burgdorferi sensu lato and is prevalent in northeastern Asia, particularly in the forested area of Northeastern China. However, a lack of systematic data on the spatial distribution of B. burgdorferi in this region hinders the prediction of its transmission risk across the landscape. Methods: To provide an updated overview and establish a comprehensive spatial distribution database, we conducted a systematic review of literature published between 2000 and 2022. We collected and compiled relevant data on B. burgdorferi in Northeastern China and its neighbouring regions, outlining its distribution in ticks, wild animals, livestock and humans. Spatial analysis was performed to identify spatial clusters of tick positivity and host infection rates. Results: From a total of 1823 literature, we selected 110 references to compile 626 detection records of B. burgdorferi, including 288 in ticks, 109 in wildlife, 111 in livestock and domestic animals and 100 in humans. The average detection rate of B. burgdorferi in ticks was approximately 20%, with wildlife, livestock and domestic animal host positivity rates below 50% and human seroprevalence rates varying from 0.94% to 44.18%. Conclusions: The study identified the presence of 17 tick species and ten genotypes of B. burgdorferi in the region, indicating a broad distribution. Notably, B. burgdorferi exhibited notable clustering, particularly in the central and eastern areas of Jilin Province, warranting further investigation. [ABSTRACT FROM AUTHOR]

Aims: Rabies virus (RV) is endemic in some Arabian countries. However, it is difficult to control RV without understanding the epidemiological evolution of endemic RV isolates. The current study aimed to characterize RV from domestic and wild animal clinical cases in Oman. Methods and Results: Twelve brain samples from domestic (Five camels, three goats and one cattle) and wild animals (Two foxes and one honey badger) were investigated from different locations in Oman between 2017 and 2020. All samples were confirmed by RV nucleoprotein (N) gene‐specific primers. Seven out of the 12 amplified samples were successfully sequenced and subjected to sequence and phylogenetic analysis. The detected RVs shared an in‐between 96.8%–98.7% and 96.9%–99% nucleotide and amino acid identities, respectively. However, the wild animal RVs shared only 92.6%–93.9% and 95.9% nucleotide and amino acid identities with the domestic animal RVs, respectively. Negri bodies were detected histologically in six brain samples from camels (n = 3), goats (n = 1) and foxes (n = 2). The RVs from domestic animals shared 97%–98.7% and 98%–100% nucleotide and amino acid identities with the previously published fox RVs from Oman and Gulf countries. Phylogenetic analysis suggested that all RV sequences belong to a distinct clade confined to the previously reported clade V within the Middle Eastern Cluster. Conclusions: As indicated by the analysis of RVs from different locations between 2017 and 2020, a genetic variant isolated to the Gulf region may exist within the Middle East clade. Moreover, it appears that new RV lineages are emerging rapidly within this region. Therefore, a comprehensive genomic and phylogenetic analysis of the circulating RV is important for the development of future prevention and control strategies. [ABSTRACT FROM AUTHOR]

A 6-month-old intact male Dalmatian mix puppy was presented for the evaluation of left maxillary swelling due to a suspected cyst and an unerupted left maxillary canine tooth. Removal of the unerupted left maxillary canine tooth (204) and enucleation of the cyst was performed, followed by histological analysis, which identified the maxillary swelling to be a cystic ameloblastic fibroma. Ameloblastic fibromas are rare in companion animals, and to the best of the authors' knowledge, this is the first cystic variant reported in dogs. The clinical, radiographic, cone beam computed tomography, and histological findings of this case are discussed and compared with the findings of previously documented human and domestic animal cases. [ABSTRACT FROM AUTHOR]

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Copyright of Veterinarska Stanica is the property of Croatian Veterinary Institute and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Objectives: This scoping review is designed to understand the role of pet ownership in the lives of people living in the community with dementia. Method: A five-stage framework for conducting a scoping review guided the review. Two research questions framed the study. Nine databases were searched, with six papers meeting the criteria for detailed review. Results: Pets can play a central role in the lives of people living with dementia. These relationships can be profound and can provide companionship and a sense of purpose. The activities associated with pet ownership and possible benefits for the person living with dementia have been explored to varying degrees and some benefits have been shown regarding the impact on physical and mental well-being. However, little is known about the challenges that may be faced when caring for a pet. Conclusion: Despite the importance of pet ownership, experiences of ownership documented among people living with dementia is limited. Still, the studies indicate how pet ownership can support people to remain socially engaged. Future studies should seek to gain a broader understanding of pet ownership across environments such as care homes and hospitals and in the context of social citizenship, active participation and living well. Creative research methods should be adopted to support the inclusion of people living with dementia in research. [ABSTRACT FROM AUTHOR]

Background: The mammalian Natural Killer Complex (NKC) harbors genes and gene families encoding a variety of C-type lectin-like proteins expressed on various immune cells. The NKC is a complex genomic region well-characterized in mice, humans and domestic animals. The major limitations of automatic annotation of the NKC in non-model animals include short-read based sequencing, methods of assembling highly homologous and repetitive sequences, orthologues missing from reference databases and weak expression. In this situation, manual annotations of complex genomic regions are necessary. Methods: This study presents a manual annotation of the genomic structure of the NKC region in a high-quality reference genome of the domestic cat and compares it with other felid species and with representatives of other carnivore families. Reference genomes of Carnivora, irrespective of sequencing and assembly methods, were screened by BLAST to retrieve information on their killer cell lectin-like receptor (KLR) gene content. Phylogenetic analysis of in silico translated proteins of expanded subfamilies was carried out. Results: The overall genomic structure of the NKC in Carnivora is rather conservative in terms of its C-type lectin receptor gene content. A novel KLRH-like gene subfamily (KLRL) was identified in all Carnivora and a novel KLRJ-like gene was annotated in the Mustelidae. In all six families studied, one subfamily (KLRC) expanded and experienced pseudogenization. The KLRH gene subfamily expanded in all carnivore families except the Canidae. The KLRL gene subfamily expanded in carnivore families except the Felidae and Canidae, and in the Canidae it eroded to fragments. Conclusions: Knowledge of the genomic structure and gene content of the NKC region is a prerequisite for accurate annotations of newly sequenced genomes, especially of endangered wildlife species. Identification of expressed genes, pseudogenes and gene fragments in the context of expanded gene families would allow the assessment of functionally important variability in particular species. [ABSTRACT FROM AUTHOR]

BACKGROUND: Rat eradication from islands is a very effective tool that can free entire ecosystems from the pressure of alien predators. In this study we present the case study of Ventotene (Ponziane Archipelago, central Italy), which to date is the island with by far the greatest number of human inhabitants ever freed from the negative implications of rats. Rat eradication was carried out in the framework of the Life PonDerat project, co‐financed by the European Union. Besides considering the conservation benefits due to the removal of rats, we also considered the socio‐economic and pathogenic impacts from introduced rats. RESULTS: The overall economic cost of the rats was quantified to be at least €18 500 per year to the residents of the island. Several zoonotic pathogens were detected in the rat population prior to eradication. A reduction in the rodenticide distributed over time on the island was also estimated. Identifying the origin of the rat population allowed for the development of more targeted and effective biosecurity measures. The eradication effort was challenged by the presence of domestic animals and variability in support for baiting in urbanised areas. CONCLUSIONS: The results of this study open up new perspectives on island restoration projects. We demonstrated the cost‐effectiveness of the action, including ecosystem restoration, reduction of rat impacts in agricultural systems, and improving overall health and food safety. Our findings will have implications for similar interventions on other islands, potentially bringing significant benefits. © 2023 Society of Chemical Industry. [ABSTRACT FROM AUTHOR]

Introduction: Mammalian reproductive and somatic development is regulated by steroid hormones, growth hormone (GH), and insulin-like growth factor-1 (IGF-1). Based largely on information from humans, model organisms, and domesticated animals, testosterone (T) and the GH/IGF-1 system activate sexually differentiated development, promoting male-biased growth, often at a cost to health and survivorship. To test if augmented prenatal androgen exposure in females produces similar developmental patterns and trade-offs, we examine maternal effects in wild meerkats (Suricata suricatta), a non-model species in which adult females naturally, albeit differentially by status, express exceptionally high androgen concentrations, particularly during pregnancy. In this cooperative breeder, the early growth of daughters predicts future breeding status and reproductive success. Methods: We examine effects of normative and experimentally induced variation in maternal androgens on the ontogenetic patterns in offspring reproductive hormones (androstenedione, A4; T; estradiol, E2), IGF-1, growth from pup emergence at 1 month to puberty at 1 year, and survivorship. Specifically, we compare the male and female offspring of dominant control (DC or high-T), subordinate control (SC or lower-T), and dominant treated (DT or blocked-T) dams, the latter having experienced antiandrogen treatment in late gestation. Results: Meerkat offspring showed sex differences in absolute T and IGF-1 concentrations, developmental rates of A4 and E2 expression, and survivorship -- effects that were sometimes socially or environmentally modulated. Atypical for mammals were the early male bias in T that disappeared by puberty, the absence of sex differences in A4 and E2, and the female bias in IGF-1. Food availability was linked to steroid concentrations in females and to IGF-1, potentially growth, and survival in both sexes. Maternal treatment significantly affected rates of T, E2, and IGF-1 expression, and weight, with marginal effects on survivorship; offspring of DT dams showed peak IGF-1 concentrations and the best survivorship. Discussion: Maternal effects thus impact offspring development in meerkats, with associated trade-offs: Whereas prenatal androgens modify postnatal reproductive and somatic physiology, benefits associated with enhanced competitiveness in DC lineages may have initial costs of reduced IGF-1, delay in weight gain, and decreased survivorship. These novel data further confirm the different evolutionary and mechanistic pathways to cooperative breeding and call for greater consideration of natural endocrine variation in both sexes. [ABSTRACT FROM AUTHOR]

Early life experiences can have lasting impacts on an animal's development. Extensive research evidence aligns across both human and non-human rodent and primate laboratory animals showing negative impacts of early life adversity such as impairments in neurological and behavioural development. Farmed animals experience a range of adversities across their production lifetimes, often early in life, including species atypical social groupings, invasive husbandry procedures, and transport. Correspondingly, farmed animals also demonstrate a wide range of impairments such as stereotypic, injurious, and other abnormal behaviours. An individual, however, needs to experience adversity to be able to develop resilience and coping mechanisms that facilitate dealing with challenges later in life. Not all individuals will experience stress vulnerability following adversity, with some individuals instead developing stress resilience. This mini review collates evidence on the positive effects of early life adversity on improving adaptability in farmed species, both terrestrial and aquatic. While evidence across farmed animal species is currently much less than for humans, laboratory rodents or non-human primates, similar patterns emerge where mild adversity early in life, can improve the adaptability of the animal in the face of future stressors. Many views of optimised welfare posit that farmed animals should be housed in as natural environments as possible to limit many of the typical adversities they face. However, strategic mild exposure to early life adversity may facilitate improved animal welfare under intensive commercial farming conditions. Future research into this area could provide management tools to better predict and promote stress resilience over stress vulnerability. [ABSTRACT FROM AUTHOR]

Background: Leptospirosis is one of the most common zoonoses in the world which is associated with a severe febrile disease in humans causing a variety of syndromes including meningitis, interstitial nephritis, hepatitis, and sometimes death. Leptospirosis is caused by different pathogenic Leptospira species divided into almost 30 serogroups and more than 300 serovars which are carried by some animal asymptomatic chronic infections. Humans can become infected through direct contact with animal urine or indirectly by coming into contact with fresh water or mud contaminated with urine. Methodology/Principal findings: In this research, we looked for leptospiral DNA in urine samples from dogs living in a rural, low-income and highly endemic community in the coast of Ecuador. We used molecular biology and next-generation sequencing for the detection. Our results showed evidence of two Leptospira species, L interrogans and L. santarosai, genomes in three dogs. Conclusions/Significance: It has been widely known that animal carriers are typically infected with a single leptospiral strain. However, recent reports, including the present one, indicate that carrier animals may be coinfected with two or more leptospiral species. [ABSTRACT FROM AUTHOR]

Over the past two decades, farm animal-assisted therapies have become popular. However, the effects of farm animals on healthy people's mental states have not yet been investigated. In Study 1, we aimed to explore whether positive effects of human-animal interaction (HAI) can be detected in healthy farm volunteers even after short-term (2–3 hours) work with goats and goatlings on a goat farm. We found that the participants' state anxiety decreased (p < 0.001) while their trust levels increased (p < 0.001) after interacting with goats. Nevertheless, it is possible that time spent in nature alone can have a beneficial effect on well-being. Therefore, in Study 2, we compared the results of Study 1 with those of a similar short-term outdoor treatment, walking in a botanical garden as a visitor. Similar but smaller effects were found for garden visitors. Decreases in anxiety scores and increases in trust scores were more pronounced in farm volunteers (anxiety: 25% vs 13%, p < 0.001; trust: 13% vs 3%, p = 0.002) after the treatments. Overall, the results suggest that the novel experience of interacting with goats and goatlings has a more positive effect on the mental state of healthy humans than walking in a botanical garden. This finding offer a strong foundation for developing animal assisted therapy methods for individuals with short or long term mental problems, but they can also enhance the wellbeing of mentally healthy people. [ABSTRACT FROM AUTHOR]

Background: Potentially zoonotic pathogens have been previously detected in bat-associated ticks, but their role in disease transmission and their frequency of feeding on non-bat hosts is poorly known. Methodology/Principal findings: We used molecular blood meal analysis to reveal feeding patterns of the bat-associated tick species Ixodes ariadnae, I. simplex, and I. vespertilionis collected from cave and mine walls in Central and Southeastern Europe. Vertebrate DNA, predominantly from bats, was detected in 43.5% of the samples (70 of 161 ticks) but in these ticks we also detected the DNA of non-chiropteran hosts, such as dog, Canis lupus familiaris, wild boar, Sus scrofa, and horse, Equus caballus, suggesting that bat-associated ticks may exhibit a much broader host range than previously thought, including domestic and wild mammals. Furthermore, we detected the zoonotic bacteria Neoehrlichia mikurensis in bat ticks for the first time, and other bacteria, such as Bartonella and Wolbachia. Conclusions/Significance: In the light of these findings, the role of bat ticks as disease vectors should be urgently re-evaluated in more diverse host systems, as they may contribute to pathogen transmission between bats and non-chiropteran hosts. Author summary: Ticks found on bats might carry diseases that can be important for both animal and human health. In this study we collected three species of bat ticks and used molecular methods to demonstrate their feeding habits and to explore the pathogens they carry. We found that most of the ticks had been feeding on bats, but we also found evidence of blood meals from domestic and wild animals, such as dogs, wild boars, and horses. We also found several bacteria in these ticks that can infect or potentially infect humans and animals. These results indicate that bat ticks could be a potential link between bats and non-bat hosts and may help spread diseases between them. Learning about how ticks interact with different host species is critical to understanding the ecology and spread of wildlife disease. [ABSTRACT FROM AUTHOR]

ABSTRACT Abomasal ulcers are a challenge in animal farming, affecting health, welfare, and productivity. Omeprazole's (OPZ) efficacy in treating these ulcers is known, but data on its pharmacokinetics (PK) in adult goats and sheep are lacking. The purpose of this research was to investigate and contrast OPZ's PK in these animals following a single intravenous (IV, 1 mg/kg) and subcutaneous (SC, 2 mg/kg) doses. Sheep and goats had similar exposure levels for all administration routes, with no significant AUC(0‐∞)D variations. Half‐life was short in both species (sheep: 0.20 h; goats: 0.31 h). Goats had a higher volume of distribution after IV administration. Clearance was rapid, and extraction ratio values were high for both goats and sheep (43% and 30%, respectively). SC administration showed similarities in Cmax and Tmax values between species. Both goats and sheep had high bioavailability (about 80%) levels and comparable mean absorption times (MAT). Despite some PK parameters' variances, systemic exposure to OPZ is similar in sheep and goats. SC administration's high bioavailability suggests it as a convenient field application route. Further investigations are needed to understand OPZ's effectiveness in small ruminants with abomasal ulcers and improve dosing regimens for clinical use. [ABSTRACT FROM AUTHOR]

Central European forests have been shaped by complex human interactions throughout the Holocene, with significant changes following the introduction of domesticated animals in the Neolithic (∼7.5–6.0 ka before present [BP]). However, understanding early pastoral practices and their impact on forests is limited by methods for detecting animal movement across past landscapes. Here, we examine ancient sedimentary DNA (seda DNA) preserved at the Velký Mamuťák rock shelter in northern Bohemia (Czech Republic), which has been a forested enclave since the early Holocene. We find that domesticated animals, their associated microbiomes, and plants potentially gathered for fodder have clear representation by the Late Neolithic, around 6.0 ka BP, and persist throughout the Bronze Age into recent times. We identify a change in dominant grazing species from sheep to pigs in the Bronze Age (∼4.1–3.0 ka BP) and interpret the impact this had in the mid-Holocene retrogressions that still define the structure of Central European forests today. This study highlights the ability of ancient metagenomics to bridge archaeological and paleoecological methods and provide an enhanced perspective on the roots of the "Anthropocene." • We analyze metagenomic data from a rock shelter using sedimentary ancient DNA • Ancient DNA reveals domesticated animals and their associated microbiomes • Phylogenetic analyses detect mitochondrial haplogroups in domestic sheep and cattle • seda DNA can help improve the understanding of our herding practices and forest management Sedimentary ancient DNA buried underneath a rock shelter reveals shifts in the presence of domesticated animals, associated microbiomes, and plants gathered for fodder from the Late Neolithic to the Bronze Age. Zampirolo et al. highlight the potential of using ancient DNA from rock shelter deposits to infer past human-environment interactions. [ABSTRACT FROM AUTHOR]

A gene bank for farm animal genetic resources (FAGR) is an important facility for the diversity conservation of FAGR. The primary purpose of gene banks for FAGR is the reconstruction of those breeds. With the advent of the post-genomic era, gene banks for FAGR have increasingly become an infrastructure for the support of livestock research and animal breeding, and a platform for international research collaboration. China is one of the richest countries in the world in terms of FAGR. Chinese National Gene Banks for FAGR (CNGBFs) have an important legal status and play an important function in the Chinese FAGR protection system. In this paper, we reviewed the current situation of CNGBFs construction, and systematically collected and analyzed legal rules related to CNGBFs. As results, those legal rules were categorized into two types: (a) organization and management, (b) activities. We summarized problems existing in the current legal rules for CNGBFs from three levels: institution, practical operation, and digital development. Improvement directions of legal rules regarding CNGBFs are proposed. They include clarifying the utilization function of CNGBFs and the ownership of FAGR in CNGBFs. Moreover, improving the mechanism of administrative management, rules on domestic and international access and benefit-sharing, and the system of digital sequence information management are also suggested. The improvements in those legal rules will contribute to the appropriate utilization of Chinese FAGR and international collaborations. [ABSTRACT FROM AUTHOR]

Brucellosis is a zoonotic disease that can result in symptoms including infertility, abortion, testicular inflammation, and arthritis in affected sheep and cattle. The disease can spread through a range of mechanisms, making outbreaks difficult to control such that affected farms often suffer severe economic losses. In addition, humans can be affected by brucellosis, and the number of cases continues to rise annually. As rates of brucellosis in Xinjiang, China have been increasing substantially in recent years, this study was developed to assess brucellosis seroprevalence among herds of cattle, sheep, and camels in Xinjiang through the use of common diagnostic procedures like the Rose Bengal test (RBT) and PCR. A cross-sectional approach was used to analyze the seroprevalence of brucellosis across 720 total farm animals (320 camels, 250 sheep, and 150 cattle). RBT incidence rate for brucellosis was detected in 60 serum samples, 14 (4.38%) from camels, 45 (18%) from sheep, and 1 (0.67%) from cattle. PCR was performed as a confirmatory approach for these RBT-positive samples, with 55 ultimately being confirmed to be positive 13 (4.06%), 41 (16.4%), and 1 (0.67%) samples from camels, sheep, and cattle, respectively. In this survey, sheep exhibited the highest brucellosis seroprevalence using these two analytical techniques, while cattle exhibited the lowest seroprevalence and camels exhibited an intermediate rate. AMOS-PCR analyses identified Brucella melitensis as the unknown bacterium in some of these samples. These results offer new insight regarding brucellosis seroprevalence among farm animals that can be used to formulate more appropriate prevention and control policies, while also improving awareness of epidemic prevention efforts, the need for routine quarantine and disinfection, the benefits of scientific breeding and management, and approaches to improving breeding efficiency for the camel, cattle, and sheep industries. [ABSTRACT FROM AUTHOR]

Since the 1960s, more than 350,000 new chemicals have been introduced into the lives of humans and domestic animals. Many of them have become part of modern life and some are affecting nature as pollutants. Yet, our comprehension of their potential health risks for both humans and animals remains partial. The “epithelial barrier theory” suggests that genetic predisposition and exposure to diverse factors damaging the epithelial barriers contribute to the emergence of allergic and autoimmune conditions. Impaired epithelial barriers, microbial dysbiosis, and tissue inflammation have been observed in a high number of mucosal inflammatory, autoimmune and neuropsychiatric diseases, many of which showed increased prevalence in the last decades. Pets, especially cats and dogs, share living spaces with humans and are exposed to household cleaners, personal care products, air pollutants, and microplastics. The utilisation of cosmetic products and food additives for pets is on the rise, unfortunately, accompanied by less rigorous safety regulations than those governing human products. In this review, we explore the implications of disruptions in epithelial barriers on the well‐being of companion animals, drawing comparisons with humans, and endeavour to elucidate the spectrum of diseases that afflict them. In addition, future research areas with the interconnectedness of human, animal, and environmental well‐being are highlighted in line with the “One Health” concept. [ABSTRACT FROM AUTHOR]

Background: The mammalian Natural Killer Complex (NKC) harbors genes and gene families encoding a variety of C-type lectin-like proteins expressed on various immune cells. The NKC is a complex genomic region well-characterized in mice, humans and domestic animals. The major limitations of automatic annotation of the NKC in non-model animals include short-read based sequencing, methods of assembling highly homologous and repetitive sequences, orthologues missing from reference databases and weak expression. In this situation, manual annotations of complex genomic regions are necessary. Methods: This study presents a manual annotation of the genomic structure of the NKC region in a high-quality reference genome of the domestic cat and compares it with other felid species and with representatives of other carnivore families. Reference genomes of Carnivora, irrespective of sequencing and assembly methods, were screened by BLAST to retrieve information on their killer cell lectin-like receptor (KLR) gene content. Phylogenetic analysis of in silico translated proteins of expanded subfamilies was carried out. Results: The overall genomic structure of the NKC in Carnivora is rather conservative in terms of its C-type lectin receptor gene content. A novel KLRH-like gene subfamily (KLRL) was identified in all Carnivora and a novel KLRJ-like gene was annotated in the Mustelidae. In all six families studied, one subfamily (KLRC) expanded and experienced pseudogenization. The KLRH gene subfamily expanded in all carnivore families except the Canidae. The KLRL gene subfamily expanded in carnivore families except the Felidae and Canidae, and in the Canidae it eroded to fragments. Conclusions: Knowledge of the genomic structure and gene content of the NKC region is a prerequisite for accurate annotations of newly sequenced genomes, especially of endangered wildlife species. Identification of expressed genes, pseudogenes and gene fragments in the context of expanded gene families would allow the assessment of functionally important variability in particular species. [ABSTRACT FROM AUTHOR]

Simple Summary: Cryopreserved semen, widely utilized in the artificial insemination of domestic animals, encounters challenges due to oxidative damage. This study investigated the effects of the antioxidant PQQ on the semen quality of Holstein bulls during cryopreservation and elucidated the associated mechanisms. Our findings revealed that PQQ significantly enhanced sperm motility, membrane integrity, acrosome integrity, and ATP levels, while simultaneously reducing MDA and ROS levels. Additionally, increased PGAM2, CAPZB, CAT, SOD1, and GPX1 protein expressions were also observed in the PQQ-treated group. Based on these results, PQQ not only improved semen quality but also mitigated oxidative stress, thereby enhancing the efficacy of sperm cryopreservation in bulls. Cryopreserved semen is extensively utilized in the artificial insemination (AI) of domestic animals; however, suboptimal conception rates due to oxidative damage following AI continue to pose a challenge. The present study investigated the effects of Pyrroroquinoline Quinone (PQQ), a novel antioxidant, on the semen quality of Holstein bulls during cryopreservation, as well as its potential molecular mechanisms. Semen samples were diluted with varying concentrations of PQQ (0, 50 μmol/L, 100 μmol/L, 150 μmol/L) prior to cryopreservation. Following the freeze–thaw process, a comprehensive evaluation was conducted to assess sperm motility, plasma membrane integrity, acrosome integrity, and the levels of reactive oxygen species (ROS), malondialdehyde (MDA), and adenosine triphosphate (ATP). Western blot analysis was employed to examine the levels of proteins including PGAM2, CAPZB, CAT, SOD1, and GPX1. Notably, the inclusion of 100 μmol/L PQQ significantly enhanced sperm motility, membrane integrity, and acrosome integrity post freeze–thawing (p < 0.05). Furthermore, the group treated with 100 μmol/L PQQ exhibited reduced levels of MDA and ROS (p < 0.05), while ATP levels were significantly elevated (p < 0.05). Interestingly, treatment with 100 μmol/L PQQ resulted in decreased consumption of PGAM2, CAPZB, CAT, SOD1, and GPX1 proteins in sperm after freeze–thawing, compared to the control group (p < 0.05). These findings indicate that PQQ treatment enhances the quality of bull semen, mitigates oxidative stress damage, and ultimately improves the efficacy of sperm cryopreservation. [ABSTRACT FROM AUTHOR]

Simple Summary: The Jhalana Reserve Forest (JRF) is located in the heart of Jaipur, the capital of Rajasthan in India. The JRF was declared a reserve forest in 2017 by the Rajasthan Forest Department. However, the authorities needed baseline data to identify and understand the biodiversity in the JRF. It is paramount to have this data collated to understand the ecosystem and changes that occur in subsequent years. The human population from the fringe villages was observed to intrude into the reserve. The authorities constructed a wall of 3 m height to curtail this intrusion. We studied and recorded the mammal and bird species in the JRF over 2 years, 2017–2018 and 2018–2019. In the first of its studies in the JRF, camera traps were placed at 17 artificial waterholes. We recorded 39 species (bird species—18, mammal species—14, domestic species—6, and human activity). The Indian Leopard is the apex predator in the JRF, and we identified 25 individuals during our study period. The human and domestic animal activity was reduced considerably after the wall was built. Studies involving the documentation of species are critical for the authorities to implement conservation strategies. We utilized camera traps to evaluate animal diversity, relative abundance, and the extent of anthropogenic activities in the Jhalana Reserve Forest (JRF), located in Jaipur, with a population of 3.9 million people. Between November 2017 and November 2019, camera traps were strategically deployed in the tourist zone and peripheral areas, capturing 16,328 photos. This study represents the first comprehensive baseline assessment of animal diversity in the JRF, documenting 39 species, including 18 bird species, 14 mammals, and 6 domestic species, alongside human activity. Among the 14 mammal species, 7 were carnivores. Notably, we identified 25 individual Indian leopards (Panthera pardus fusca) during 2017–2018, comprising 8 males and 17 females, highlighting the leopard as the apex predator in the JRF. Concurrently, domestic animals accompanied by humans were observed within the JRF. However, rigorous conservation efforts and patrols by the Rajasthan Forest Department resulted in a notable decline in human activity, from 28.04% in 2017–2018 to 3.92% in 2018–2019, with domestic animal activity reaching zero in the latter period. Consequently, the relative abundance of wildlife species increased during the study period of 2018–2019, underscoring the positive impact of conservation strategies implemented by authorities. Our findings establish that camera-trapping methodology collates definitive baseline data, assesses mammal diversity, and evaluates relative abundance in reserve forests within human-dominated landscapes. We strongly recommend a further study to assess the avifauna diversity. This study provides critical insights to inform the development and implementation of conservation strategies in similar protected areas. [ABSTRACT FROM AUTHOR]

Simple Summary: The demand for animal-assisted services (AAS) has increased in the past decades. The participation of supporting animals in complementary therapy for humans is a developing area. While dogs and horses are the most widely utilized species in AAS, several unconventional species have already been involved, although there is a lack of information about the effects of the stress they may experience. During the interventions, animals may experience stress that can potentially violate aspects of animal welfare. This review seeks to identify criteria for selecting unconventional animal species, such as guinea pigs, rabbits, farm animals, alpacas, donkeys, reptiles, aquarium fishes, and dolphins, for AAS, considering factors such as temperament, trainability, human–animal bond potential, stress measurement, and stress mitigation strategies. Despite the growing interest in AAS, our literature review underscores the scarcity of research exploring their effects on unconventional animal species, especially in stress measurement; therefore, more extensive studies should be conducted, for instance, measuring biochemical parameters, such as cortisol. The participation of animals during complementary therapy for humans is a developing area. Dogs and horses are the most frequent partner species in animal-assisted services, but several unconventional species have also been involved, although there is a lack of information about the stress they experience caused by AAS. We conducted a comprehensive literature search, analyzing 135 articles with the purpose of investigating the effects of AAS on unconventional species such as guinea pigs, rabbits, farm animals, alpacas, donkeys, reptiles, aquarium fishes, and dolphins. We found that the relevant articles emphasize investigating the impact of animal-assisted interventions on humans, and they generally report positive outcomes. Limited data is available concerning the potential consequences the interventions may have on the animals. Therefore, it is our conclusion that more extensive studies should be conducted to get adequate information on stress experienced by animals during AAS, such as the measurement of biochemical parameters such as cortisol. Hence, meeting animal welfare considerations in addition to human interests could serve as a basis for the recommended methodology for therapies. [ABSTRACT FROM AUTHOR]

Environmental enrichment could lower stress in farm animals; however, inadequate data is offered on the level of productivity and welfare indicators in rabbits reared with or without environmental enrichment. 84 young White New Zealand rabbits were used to identify two examples of environmental enrichment (gnawing sticks and sibling) influences on the behavior, production, and stress level of growing rabbits. Two experiments were done; In experiment one; 36 young rabbits were used to test the effect of sex and sibling relationship on the behaviour and weight gain of the growing rabbits, four groups G1 male sibling, G2 male unsibling, G3 female sibling, and G4 female unsibling. In experiment two; 48 young rabbits were used in two steps; in the first step of the trial the preference test of gnawing sticks tree species was performed by using visual evaluation and the second step was to test the influence of the prefered gnawing sticks tree species in the 1st step on the behaviour, performance, and stress level of growing rabbits. They were classified in to two groups G (1) with the prefered G.S and G (2) rabbits without any gnawing sticks. The behaviour of the rabbit groups was recorded at 4 week old till 11 weeks old using focal sampling technique. Weekly body weight and serum cortisole level at 6 and 10 weeks in both experiment was recorded. Gnawing sticks and sibling housing during the growing period result in improvement in growing rabbits behavior (feeding, self grooming, and social smelling), lowering biting wire and feeder, and decrease cortisole hormone level. So that this study recommends to use them for growing rabbit cages during the growing periods to override the barren environment adverse effects. [ABSTRACT FROM AUTHOR]

The Hengduan Mountains are located on the southeastern edge of the Qinghai‐Tibet Plateau, where farmers began to settle 6000 years ago. Animal husbandry plays a significant role in the sustenance economies of agricultural civilizations. It is unclear how Neolithic people acquired animal resources in the Hengduan Mountains. We explore animal geographical origins and feeding practices using the strontium, carbon, and oxygen isotope composition of tooth enamel at the Guijiabao and Yingpanshan sites during the Neolithic Age in the Hengduan Mountains. Multi‐isotopic evidence demonstrates that animals originate from several different regions with diverse foods. Guijiabao domestic dogs and pigs with a mixed C3/C4 diet share a similarly broad dietary spectrum with humans as enclosed animals, but the other pigs are wild boars with a C3 diet, likely free‐range animals or directly captured as meat resources. Yingpanshan dogs and pigs are both domestic animals with a mixed C3/C4 or C4‐based diet, but pigs with different 87Sr/86Sr ratios are likely raised by dispersed feeding modes. The inhabitants had diverse approaches for obtaining animal resources, including husbandry, hunting, and exchange. Diverse animal subsistence patterns are closely related to the complex geographical environment, reflecting the adaptation of farmers living in the high mountain valley regions. [ABSTRACT FROM AUTHOR]

Visual arts have witnessed the emergence of various roles and themes throughout history, resulting in the development of diverse methods and platforms within the industry. In the realm of urban music, specifically in Iran, musical instruments are adorned with various images, becoming integral accessories to this art form. In the 6th century AH, along the banks of the Aras River in the region historically known as the Kingdom of Sharvan, Khaghani Sharvani, a poet and musicologist, highlighted the unique role of Daf, a percussion instrument adorned with depictions of domestic and wild animals, especially in hunting situations. This article employed a historical-analytical method and relied on library studies to explore this topic. Multiple factors contribute to the significance of Daf in this context, making it difficult to pinpoint a single definitive reason. However, considering the historical connection between similar practices among the shamans of Siberia and the Caucasus, as well as Khaghani Sharvani's emphasis on the association between Daf and the hunting grounds of the kings of Sharvan, this explanation emerges as the most compelling and informative among the various reasons that can be discussed. [ABSTRACT FROM AUTHOR]

Low levels of essential mineral elements such as cobalt, copper, and iron, in organisms reduce immune function, increasing the chances of parasitic infection. This phenomenon has been demonstrated widely in domestic animals but rarely in wildlife. In this study, we used data from 7‐ to 9‐month‐old roe deer (Capreolus capreolus), living in two different populations facing contrasting environmental conditions (Trois‐Fontaines and Chizé), to investigate whether the parasitic and immunological statuses could be related to essential element status. Between 2016 and 2019, we collected feces to measure parasite burdens (gastrointestinal and pulmonary nematodes), blood to measure immunological parameters (globulins and white blood cells), and hair to assess the concentration of 11 essential elements (calcium [Ca], chromium [Cr], cobalt [Co], copper [Cu], iron [Fe], magnesium [Mg], manganese [Mn], potassium [K], molybdenum [Mo], selenium [Se], and zinc [Zn]). The results showed first heterogeneity in the individual phenotypes of the two populations. Roe deer with low body mass had high concentrations of all the essential elements (in particular, Ca, Fe, Cu, K, and Mn), a high parasitic burden, and high concentrations of globulins. An association between high concentrations of essential elements and a high parasite burden was found at the two study sites despite markedly different environmental conditions. A relationship between essential element concentrations and immune parameters was also detected, with more basophils and globulins being associated with high concentrations of essential trace elements (i.e., Ca, Fe, Cu, and, to a lesser extent, Se, Cr, and Zn). These results suggest that young individuals with low body mass and high parasitism may select feeding resources rich in mineral elements, which may improve their ability to control the infestation and/or mitigate the negative consequences of parasites by maintaining immune system functions. [ABSTRACT FROM AUTHOR]

Simple Summary: The media supporting selected mammalian preimplantation embryos in vitro have played an essential role in the advancement of assisted reproductive technology (ART). ART has in turn transformed biomedical research with genetically modified, disease-specific animal models, agriculture with genetically modified farm animals and human reproductive health with infertility treatment. However, the current culture systems still cannot replicate the in vivo environment, and embryos derived from in vitro systems are poor in quality compared to their in vivo counterparts. The objective of this review is to discuss the lineage of chemically defined media from simple salt solutions to modern media commonly used in embryo cultures for laboratory animals (mice and rats) and farm animals (pigs and cattle) and provide some insights for future improvements. This discussion provides readers with knowledge regarding the evolution of media used to support the development of preimplantation embryos in vitro and aims to inspire interest in the development of media to better support embryo development in vitro. Assisted reproductive technology has revolutionized our ability to genetically manipulate, maintain and rederive laboratory animals of biomedical importance; manipulate animal reproduction or genetics to boost production of farm animals; and improve human reproductive health. The media for in vitro manipulation and the culture of embryos play a critical role in the development of assisted reproductive technology. In this review, the evolution of culture media supporting embryo development in vitro from selected animal species, laboratory animals (mice and rats) and farm animals (pigs and cattle), will be discussed with a focus on the development of chemically defined media. [ABSTRACT FROM AUTHOR]

Background In the last decade, veterinary antimicrobial usage (AMU) and antimicrobial resistance (AMR) among indicator bacteria in livestock have decreased substantially in the Netherlands. The extent to which this decrease has affected AMR levels among human infections remains unclear. Objectives To assess the association between AMU in livestock and AMR in Escherichia coli isolates from human urinary tract infections (UTIs). Methods Data on AMR and AMU between 2009 and 2020 from Dutch national surveillance programmes for humans and livestock were used. Associations between AMU in four major livestock sectors and AMR in humans were assessed for 10 antimicrobial classes and the ESBL resistance profile, using logistic regression analysis. Associations between AMU and AMR in livestock, between AMR in livestock and in humans, and between AMU and AMR in humans were also assessed. Results Statistical significance was reached for 16/31 of the tested associations between AMU in livestock and AMR in human E. coli UTIs. Of the significant associations, 11 were positive (OR 1.01–1.24), whereas 5 were negative (OR 0.96–0.99). All associations between human AMU and AMR in E. coli isolates from UTIs were positive and statistically significant. Weak but significant positive correlations were also observed between livestock AMR and human AMR. Conclusions Although several significant associations between AMU in livestock and AMR in human UTIs caused by E. coli were observed, the associations between AMU and AMR were generally stronger within the human and animal populations. This indicates that potential zoonotic spread of AMR in E. coli causing human UTIs from livestock sources is limited. [ABSTRACT FROM AUTHOR]

Simple Summary: Peccaries are distributed from the southern United States to southern South America. Although they were initially described as being from the pig genus Sus, they are very different. This species is currently being used on commercial farms as a source of animal protein; however, there are no studies about their salivary glands, which are important for digestion and in developing feeding strategies. Thus, this work aims to study the major salivary gland morphology of peccaries during their growth. During growth, the parotid enlarges and the mandibular gland loses weight. Histologically, the parotid has serous production, and the sublingual has mucus production, like most species; however, the mandibular gland produces mucus, unlike other animals, including pigs, which produce seromucous secretion. Histochemically, the parotid produces more acidic mucins than pigs and it undergoes maturation during development; the mandibular, especially the sublingual gland, produces more acidic and basic mucopolysaccharides than pigs. The major salivary glands reacted positively to different lecithins and showed a stronger reaction than in pigs. We conclude that peccaries have a salivary secretion that facilitates the digestion of carbohydrates, improving digestibility and performance. Their biometric characteristics and positive reactions to lecithins facilitate adaptation to foods with antinutritional factors, making them a promising production animal. This work aims to study the major salivary gland morphology of peccaries during their growth. The glands were analyzed using macroscopic description, light microscopy, electron microscopy, histochemistry, and immunohistochemistry. Topographically, the salivary glands resemble other animals, including domestic animals and pigs. During growth, the parotid enlarges and mandibular gland loses weight. Histologically, the parotid has serous production, and sublingual has mucous production, resembles most species, however, mandibular gland produces mucous, unlike other animals, including pigs, which produce seromucous secretion. Histochemically, parotid produces more acidic mucins than pigs and it undergoes maturation during development; mandibular, and especially the sublingual gland, produce more acidic and basic mucopolysaccharides than pigs. The results found with transmission and scanning electron microscopy techniques corroborate the histological and histochemistry findings. The major salivary glands were positive to different lecithins (Com-A, BSA-I-B4, WGA and PNA), which were also more positive than in pigs and sheep. We conclude that collared peccaries have a salivary secretion that facilitates the digestion of carbohydrates, and biometric characteristics and positivity to lecithins that facilitate adaptation to foods with antinutritional factors. [ABSTRACT FROM AUTHOR]

Simple Summary: Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), the virus responsible for COVID-19, primarily spreads among humans, but there have been cases of transmission between humans and animals, as well as some instances of animal-to-animal transmission. Several zoos have reported cases of large cats such as tigers and lions, gorillas, and other animal species testing positive for SARS-CoV-2, presumably due to contact with humans. White-tailed deer (WTD) are highly susceptible to SARS-CoV-2 and are prevalent throughout the United States with a high population density. Separately, there is limited research and reporting on the susceptibility of bison, elk, and other wildlife species to SARS-CoV-2. This study determines the SARS-CoV-2 seroprevalence for various captive wildlife, elk, and bison, across different regions in the United States, using several serological tests. The presence of neutralizing antibodies to the virus in cheetahs, gorillas, lions, hippopotamuses, elk, and bison indicates that they are susceptible to SARS-CoV-2 infection. This highlights the importance of continuous monitoring of the prevalence of SARS-CoV-2 antibodies in various species that come into close contact with humans. Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), the virus responsible for COVID-19, has been found to infect various domestic and wild animal species. In this study, convenience serum samples from 575 bison, 180 elk, and 147 samples from various wildlife species collected between 2020 and 2023 from several regions in the United States were analyzed for the presence of SARS-CoV-2-specific antibodies. Two commercial ELISA assays based on the inhibition of the SARS-CoV-2 receptor-binding domain (sVNT) or the nucleocapsid protein (N-ELISA) of SARS-CoV-2 were used. Positive samples from the sVNT were additionally evaluated using a conventional virus neutralization test (VNT). Our results indicated that 1.2% of bison, 2.2% of elk, and 4.1% of the other wildlife species serum samples were seropositive in the sVNT, whereas 4.2% of bison, 3.3% of elk, and 1.4% of the other captive wildlife species serum samples tested positive by the N-ELISA. Among the sVNT serum samples, two samples from bison, one sample from elk, and five serum samples from other wildlife species (one cheetah, one gorilla, two lions, and one hippopotamus) had neutralizing antibody titers in the VNT, indicating these species are susceptible to SARS-CoV-2 infection. These findings highlight the importance of broad surveillance efforts for the effective monitoring of SARS-CoV-2 in non-human hosts. [ABSTRACT FROM AUTHOR]

As one of the most important economic traits for domestic animal husbandry, skeletal muscle is regulated by an intricate molecular network. Adenosine deaminase acting on RNA (ADAR1) involves various physiological processes and diseases, such as innate immunity and the development of lung adenocarcinoma, breast cancer, gastric cancer, etc. However, its role in skeletal muscle growth requires further clarification. Here, we explored the functions of ADAR1 in the myogenic process of goat skeletal muscle satellite cells (MuSCs). The ADAR1 transcripts were noticeably enriched in goat visceral tissues compared to skeletal muscle. Additionally, its levels in slow oxidative muscles like the psoas major and minor muscles were higher than in the fast oxidative glycolytic and fast glycolytic muscles. Among the two common isoforms from ADAR1, p110 is more abundant than p150. Moreover, overexpressing ADAR1 enhanced the proliferation and myogenic differentiation of MuSCs. The mRNA-seq performed on MuSCs' knockdown of ADAR1 obtained 146 differentially expressed genes (DEGs), 87 upregulated and 59 downregulated. These DEGs were concentrated in muscle development and process pathways, such as the MAPK and cAMP signaling pathways. Furthermore, many DEGs as the key nodes defined by protein–protein interaction networks (PPI), including STAT3, MYH3/8, TGFβ2, and ACTN4, were closely related to the myogenic process. Finally, RNA immunoprecipitation combined with qPCR (RIP-qPCR) showed that ADAR1 binds to PAX7 and MyoD mRNA. This study indicates that ADAR1 promotes the myogenic development of goat MuSCs, which provides a useful scientific reference for further exploring the ADAR1-related regulatory networks underlying mammal skeletal muscle growth. [ABSTRACT FROM AUTHOR]